Patterns of Knowledge and Use of Medicinal Plants in Santiago Camotlán,Oaxaca, Mexico

Medicinal plants continue to play an important role in healthcare, both in Mexico and around the world. We investigated the relationship between various sociodemographic factors, such as age, economic activity, years of schooling, socioeconomic levels, gender, and language proficiency, and the knowledge and frequency of medicinal plant use in Santiago Camotlán, Sierra Norte of Oaxaca, with a mainly Zapotec population. In a first stage, we interviewed specialists in depth and collected and identified plants for a catalogue of the medicinal plant flora (90 species). With this catalogue, we then interviewed a sample of the general population and the specialists on knowledge and frequency of use. The relationship between the sociodemographic variables and knowledge was analyzed by multiple regression. To explore differences of knowledge within the population, we identified three groups with a two-step cluster analysis; the results were compared statistically with a Kruskal-Wallis non-parametric test and then a post hoc Dunn’s test to see if all the differences between groups were significant. Age and occupation explained 54% of the variation of knowledge. Medicinal plant knowledge in the region persists mainly because of the necessity to treat the diseases that the “doctor does not cure,” the culture-bound syndromes, and the most common ailments, malfunctions of the digestive system.     

Does Forest Scarcity Affect the Collection and Use of Firewood by Rural Communities? A Case Study in the Atlantic Forest of Northeastern Brazil

Many rural communities no longer have abundant forest areas for firewood collection. Based on this scenario, we evaluated how the scarcity of forest environments influences the collection and use of this resource in rural areas. A community located in the Atlantic Forest region of northeastern Brazil, which already lacks forest areas for firewood, was selected for field research. We observed that despite this context of forest scarcity, firewood remains the main source of fuel energy in the community. In total, 27 species of wood were cited, with an emphasis on exotic species in terms of number of citations. The perceived wood quality explained the frequency of plant use, but not the number of consumers. This can be explained by the difficulty of access to some species considered of better quality, because in the studied region, the plants are usually collected in private areas. Efforts in conservation strategies are recommended to encourage an increase in the frequency of use of the available exotic species in order to safeguard the native ones.     

The Inclusion and Selection of Medicinal Plants in Traditional Pharmacopoeias—Evidence in Support of the Diversification Hypothesis

The Inclusion and Selection of Medicinal Plants in Traditional Pharmacopoeias—Evidence in Support of the Diversification Hypothesis. An ethnobotanical study with phytochemical analyses was undertaken to examine the medicinal plants used by residents of a small rural community in northeastern Brazil. The present work tested two ideas that attempt to explain the inclusion and selection of medicinal plants in a given culture: the diversification hypothesis and the concept of versatility. The study involved 101 people and used semistructured interviews. A total of 61 plants were selected, including 25 exotic and 36 native species. Plants were classified according to their habit and analyzed for their phytochemical components. In addition, the relative importance (RI) of these plants was calculated, and a chemical diversity index (CDI) was created and applied to each of the species. Exotic and native plants were found to have significantly different occurrences of certain classes of compounds; this result supports the diversification hypothesis. It was therefore concluded that exotic plants are included in traditional pharmacopoeias to fill therapeutic vacancies that native plants cannot satisfy.     

Does ABA in the Xylem Control the Rate of Leaf Growth in Soil-Dried Maize and Sunflower Plants?

Maize (Zea mays L.) and sunflower (Helianthus annuus L.) plantswere grown in large volumes of soil and leaf growth rate wasmonitored on a daily basis. Half the plants were given a soildrying treatment and when they showed a significant restrictionof growth rate (compared to both their daily growth rate beforedrying and the average growth rate of well-watered plants onthe same day), leaf water relations were measured and xylemsap was extracted using several techniques. There was a significant negative log-linear relationship betweenthe rate of leaf growth and the concentration of ABA in thexylem for both species. There was no clear relationship betweenleaf growth rate and leaf water potential or turgor for eitherspecies. Assessment of different methods for sampling xylemsap suggests that exudates collected from stem stumps or samplescollected by pressurizing the whole root system are suitablefor estimating ABA concentration in xylem, at least with largeplants of maize or sunflower, provided the first few hundredcubic millimetres of collected sap are used for the assay. Centrifugationof sections of stems resulted in dilution of ABA in the xylemsap with sap squeezed from parenchyma tissue. This is because,at least in plants subjected to mild soil drying, the concentrationof the ABA in the xylem is far higher than that in the cellsap of stem tissue. Results support the proposal that ABA plays a major role asa chemical signal involved in the root-to-shoot communicationof the effects of soil drying. The non-hydraulic restrictionof leaf growth by a chemical signal can be explained by theextra root-sourced ABA in the xylem and may be an importantcomponent of the modification of growth and development whichresults from prolonged soil drought. Key words: Soil drying, ABA, leaf growth, Zea mays L., Helianthus annuus L.     

Does Landscape Fragmentation Influence Sex Ratio of Dioecious Plants? A Case Study of Pistacia chinensis in the Thousand-Island Lake Region of China

The Thousand-Island Lake region in Zhejiang Province, China is a highly fragmented landscape with a clear point-in-time of fragmentation as a result of flooding to form the reservoir. Islands in the artificial lake were surveyed to examine how population sex ratio of a dioecious plant specie Pistacia chinensis B. was affected by landscape fragmentation. A natural population on the mainland near the lake was also surveyed for comparison. Population size, sex ratio and diameter at breast height (DBH) of individuals were measured over 2 years. More than 1,500 individuals, distributed in 31 populations, were studied. Soil nitrogen in the different populations was measured to identify the relationship between sex ratio and micro-environmental conditions. In accordance with the results of many other reports on biased sex ratio in relation to environmental gradient, we found that poor soil nitrogen areas fostered male-biased populations. In addition, the degree of sex ratio bias increased with decreasing population size and population connectivity. The biased sex ratios were only found in younger individuals (less than 50 years old) in small populations, while a stable 1∶1 sex ratio was found in the large population on the mainland. We concluded that the effects of landscape fragmentation on the dioecious population sex ratio were mainly achieved in relation to changing soil nitrogen conditions in patches and pollen limitation within and among populations. Large populations could maintain a more suitable environment in terms of nutrient conditions and pollen flow, subsequently maintaining a stable sex ratio in dioecious plant populations. Both micro-environmental factors and spatial structure should be considered in fragmented landscape for the conservation of dioecious plant species.     

Are You HIV Invincible? A Probabilistic Study of Discordant Couples in the Context of HIV Transmission

A number of factors have been identified that are related to sexual and injecting HIV transmission. We developed a probabilistic mathematical model to put these factors together and interpret risks in the context of individual behavior among injecting drug-using (IDU) couples in St. Petersburg, Russia. Some HIV-discordant couples have unprotected sex and sometimes inject drugs together but stay discordant for a long time, while some individuals acquire HIV on the first encounter. We considered existing estimates of HIV transmission risks through injecting and sexual contacts to develop a predictive survival model for an individual who is exposed to HIV through intimate relationships. We computed simulated survival curves for a number of behavioral scenarios and discussed sources of simulated uncertainty. We then applied the model to a longitudinal study of HIV-discordant couples and validated the model’s forecast. Although individual prediction of seroconversion time appeared impossible, the ability to rank behavioral patterns in terms of HIV risk and to estimate the probability of survival HIV-free will be important to educators and counselors.     

How Does Environmental Variation Affect the Distribution of Dragonfly Larvae (Odonata) in the Amazon-Cerrado Transition Zone in Central Brazil?

This study investigated the effects of environmental variation on assemblages of dragonfly larvae (Odonata). We hypothesize that there is a significant correlation between species richness, species composition, and abundance of Odonata individuals, and habitat integrity and abiotic variables. To test this hypothesis, we sampled odonate larvae at 12 streams in the Suiá-Miçú River basin in Mato Grosso, Brazil, during three different periods of the year. Local physical and chemical variables (temperature, pH, turbidity, electrical conductivity (EC), dissolved oxygen (DO), total dissolved solids (TDS), and oxidation reduction potential (ORP)) were measured at each site using a multi-parameter probe, and integrity was assessed using the Habitat Integrity Index (HII). The variation in richness, abundance, and composition of Odonata species was related to the environmental variables analyzed, primarily by the abiotic factors pH, electrical conductivity, dissolved oxygen, total dissolved solids, and oxidation reduction potential. Our hypothesis was corroborated for the suborder Anisoptera, which showed a significant relationship with these variables, whereas Zygoptera was only related to pH. Our results show the importance of physical and chemical conditions in ecological studies using Odonata larvae as tools for the management and conservation of freshwater ecosystems.     

Does Invasion of Exotic Plants Promote Invasion of Exotic Flower Visitors? A Case Study from the Temperate Forests of the Southern Andes

Habitat disturbance, particularly of human origin, promotes the invasion of exotic plants, which in turn might foster the invasion of alien-interacting animals. Here we assess whether the invasion of exotic plants – mostly mediated by habitat disturbance – facilitates the invasion of exotic flower visitors in temperate forests of the southern Andes, Argentina. We recorded visit frequencies and the identity of visitors to the flowers of 15 native and 15 exotic plant species occurring in different highly disturbed and less disturbed habitats. We identified three alien flower visitors, the hymenopterans Apis mellifera, Bombus ruderatus, and Vespula germanica. We found significantly more visitation by exotic insects in disturbed habitats. This pattern was explained, at least in part, by the association between alien flower visitors and flowers of exotic plants, which occurred more frequently in disturbed habitats. However, this general pattern masked different responses between the two main alien flower visitors. Apis mellifera exploited almost exclusively the flowers of a subset of herbaceous exotic plants that thrive under disturbance, whereas B. ruderatus visited equally flowers of both exotic and native plants in both disturbed and undisturbed habitats. We did not find any strong evidence that flowers of exotic plants were more generalist than those of native plants, or that exotic flower visitors were more generalist than their native counterparts. Our results suggest that alien plant species could facilitate the invasion of at least some exotic flower visitors to disturbed habitats. Because flowering plants as well as flower visitors benefit from this mutualism, this association may enhance, through a positive feedback, successful establishment of both exotic partners.     

Factors Contributing to the Delay in Diagnosis and Continued Transmission of Leprosy in Brazil – An Explorative,Quantitative, Questionnaire Based Study

BackgroundLeprosy is a leading cause of preventable disability worldwide. Delay in diagnosis of patients augments the transmission of infection, and allows progression of disease and more severe disability. Delays in diagnosis greater than ten years have been reported in Brazil. To reduce this delay, it is important to identify factors that hinder patients from presenting to doctors, and those that delay doctors from diagnosing patients once they have presented. This study aimed to explore factors associated with the delayed diagnosis of leprosy in Brazil.

Methodology/ Principal Findings

This is an exploratory study using a self-constructed questionnaire delivered to patients attending three leprosy referral clinics across three states in Brazil. Data were analysed to determine associations between variables and the time taken for participants to present to the health-service, and between variables and the time taken for doctors to diagnose participants once they had presented. Participants who suspected they had leprosy but feared community isolation were 10 times more likely to wait longer before consulting a doctor for their symptoms (OR 10.37, 95% CI 2.18–49.45, p = 0.003). Participants who thought their symptoms were not serious had a threefold greater chance of waiting longer before consulting than those who did (OR 3.114, 95% CI 1.235–7.856, p = 0.016). Forty-two point six per cent of participants reported initially receiving a diagnosis besides leprosy. These had a three times greater chance of receiving a later diagnosis of leprosy compared to those not misdiagnosed or not given a diagnosis (OR 2.867, 95% CI 1.288–6.384, p = 0.010).

Conclusions/ Significance

This study implies a need for patient education regarding leprosy symptoms and the reduction of stigma to encourage patients to present. The high rate of misdiagnosis reported suggests a need to increase clinician suspicion of leprosy. Further education regarding disease symptoms in medical school curriculums may be advisable.     

Sustaining Plants and People: Traditional Q’eqchi’ Maya Botanical Knowledge and Interactive Spatial Modeling in Prioritizing Conservation of Medicinal Plants for Culturally Relative Holistic Health Promotion

Ethnobotanical surveys were conducted to locate culturally important, regionally scarce, and disappearing medicinal plants via a novel participatory methodology which involves healer-expert knowledge in interactive spatial modeling to prioritize conservation efforts and thus facilitate health promotion via medicinal plant resource sustained availability. These surveys, conducted in the Maya Mountains, Belize, generate ethnobotanical, ecological, and geospatial data on species which are used by Q’eqchi’ Maya healers in practice. Several of these mountainous species are regionally scarce and the healers are expressing difficulties in finding them for use in promotion of community health and wellness. Based on healers’ input, zones of highest probability for locating regionally scarce, disappearing, and culturally important plants in their ecosystem niches can be facilitated by interactive modeling. In the present study, this is begun by choosing three representative species to train an interactive predictive model. Model accuracy was then assessed statistically by testing for independence between predicted occurrence and actual occurrence of medicinal plants. A high level of accuracy was achieved using a small set of exemplar data. This work demonstrates the potential of combining ethnobotany and botanical spatial information with indigenous ecosystems concepts and Q’eqchi’ Maya healing knowledge via predictive modeling. Through this approach, we may identify regions where species are located and accordingly promote for prioritization and application of in situ and ex situ conservation strategies to protect them. This represents a significant step toward facilitating sustained culturally relative health promotion as well as overall enhanced ecological integrity to the region and the earth.     

The Disconnect Between Knowledge and Perceptions: A Study of Fishermen’s Local Ecological Knowledge and Their Perception of the State of Fisheries and How These Are Managed in the Dominican Republic

Human Ecology - Understanding what fishers know about the ecology of the fish they catch, and how they perceive the state and management of their fisheries can guide efforts towards more...     

Exploring the Diversity and Distribution of Neotropical Avian Malaria Parasites – A Molecular Survey from Southeast Brazil

Southeast Brazil is a neotropical region composed of a mosaic of different tropical habitats and mountain chains, which allowed for the formation of bird-rich communities with distinct ecological niches. Although this region has the potential to harbor a remarkable variety of avian parasites, there is a lack of information about the diversity of malarial parasites. We used molecular approaches to characterize the lineage diversity of Plasmodium and Haemoproteus in bird communities from three different habitats in southeast Brazil based on the prevalence, richness and composition of lineages. We observed an overall prevalence of 35.3%, with a local prevalence ranging from 17.2% to 54.8%. Moreover, no significant association between prevalence and habitat type could be verified (p>0.05). We identified 89 Plasmodium and 22 Haemoproteus lineages, with 86% of them described for the first time here, including an unusual infection of a non-columbiform host by a Haemoproteus (Haemoproteus) parasite. The composition analyses of the parasite communities showed that the lineage composition from Brazilian savannah and tropical dry forest was similar, but it was different from the lineage composition of Atlantic rainforest, reflecting the greater likeness of the former habitats with respect to seasonality and forest density. No significant effects of habitat type on lineage richness were observed based on GLM analyses. We also found that sites whose samples had a greater diversity of bird species showed a greater diversity of parasite lineages, providing evidence that areas with high bird richness also have high parasite richness. Our findings point to the importance of the neotropical region (southeast Brazil) as a major reservoir of new haemosporidian lineages.     

Gibberellin Biosynthesis in Plants and Fungi: A Case of Convergent Evolution?

As well as being phytohormones, gibberellins (GAs) are present in some fungi and bacteria. Indeed, GAs were first discovered in the fungus Gibberella fujikuroi, from which gibberellic acid (GA3) and other GAs are produced commercially. Although higher plants and the fungus produce structurally identical GAs, there are important differences in the pathways and enzymes involved. This has become particularly apparent with the identification of almost all of the genes for GA-biosynthesis in Arabidopsis thaliana and G. fujikuroi, following the sequencing of the Arabidopsis genome and the detection of a GA-biosynthesis gene cluster in the fungus. For example, 3b-hydroxylation occurs early in the pathway in G. fujikuroi and is catalyzed by a cytochrome P450 monooxygenase, whereas it is usually the final step in plants and is catalyzed by 2-oxoglutarate-dependent dioxygenases. Similarly, 20-oxidation is catalyzed by dioxygenases in plants and a cytochrome P450 in the fungus. Even where cytochrome P450s have equivalent functions in plants and Gibberella, they are unrelated in terms of amino acid sequence. These profound differences indicate that higher plants and fungi have evolved their complex biosynthetic pathways to GAs independently and not by horizontal gene transfer.     

The Dynamics of Transmission and Spatial Distribution of Malaria in Riverside Areas of Porto Velho,Rond?nia,in the Amazon Region of Brazil

The study area in Rondônia was the site of extensive malaria epidemic outbreaks in the 19^th and 20^th centuries related to environmental impacts, with large immigration flows. The present work analyzes the transmission dynamics of malaria in these areas to propose measures for avoiding epidemic outbreaks due to the construction of two Hydroelectric Power Plants. A population based baseline demographic census and a malaria prevalence follow up were performed in two river side localities in the suburbs of Porto Velho city and in its rural vicinity. The quantification and nature of malaria parasites in clinical patients and asymptomatic parasite carriers were performed using microscopic and Real Time PCR methodologies. Anopheles densities and their seasonal variation were done by monthly captures for defining HBR (hourly biting rate) values. Main results: (i) malaria among residents show the riverside profile, with population at risk represented by children and young adults; (ii) asymptomatic vivax and falciparum malaria parasite carriers correspond to around 15% of adults living in the area; (iii) vivax malaria relapses were responsible for 30% of clinical cases; (iv) malaria risk for the residents was evaluated as 20–25% for vivax and 5–7% for falciparum malaria; (v) anopheline densities shown outdoors HBR values 5 to 10 fold higher than indoors and reach 10.000 bites/person/year; (vi) very high incidence observed in one of the surveyed localities was explained by a micro epidemic outbreak affecting visitors and temporary residents. Temporary residents living in tents or shacks are accessible to outdoors transmission. Seasonal fishermen were the main group at risk in the study and were responsible for a 2.6 fold increase in the malaria incidence in the locality. This situation illustrates the danger of extensive epidemic outbreaks when thousands of workers and secondary immigrant population will arrive attracted by opportunities opened by the Hydroelectric Power Plants constructions.     

Can the Introduction of Modern Crop Varieties in their Centre of Origin Affect Local Ecological Knowledge? A Case Study of Papaya in the Yucatan Peninsula

The effect on local ecological knowledge (LEK) of introducing modern crop varieties in their centre of origin has been generally overlooked. LEK of the reproductive ecology of cultivated and wild papaya was assessed in a Mayan community using questionnaires, in-depth interviews, and participant observation. Although the Maya have managed the wild variety of papaya for several centuries, there was more LEK of the recently introduced maradol variety. Most informants were unable to differentiate male and female plants, likely because the most common variety (maradol) in contemporary home gardens is typically hermaphroditic. Informants also mentioned that, in the past, sexual expression was manipulated. We conclude that the introduction of the maradol variety has contributed to the erosion of LEK of the reproductive ecology of papaya.     

Environmental Influence on Planktonic Cladocerans and Copepods in the Floodplain of the Upper River Paraná, Brazil

The abundance of microcrustacean species recorded in plankton samples from the littoral and pelagic zones of the Upper Paran River, State of Mato Grosso do Sul, Brazil, was evaluated in relation to abiotic and biotic environmental factors. The cladocerans Bosminopsis deitersi and Bosmina hagmann i were most abundant. The presence or absence of aquatic macrophytes also influenced abundance and species composition. The true zooplankton species were most abundant at the pelagic stations, while species of Chydoridae and Macrothricidae contributed markedly to microcrustacean abundance and species richness at littoral stations. Species abundance was also strongly affected by water level variation. Microcrustaceans, mainly bosminids, were most abundant during low water. During high water, bosminids decreased in abundance while most other species increased. The higher variation of the environmental factors during high water probably explains the shift in abundance patterns. Changes in water level increased mixing of littoral and pelagic microcrustaceans between sampling stations.     

Does the Production of an Airbag Injure more People than the Airbag Saves in Traffic?

Social life cycle assessment (S‐LCA) has been discussed for some years in the LCA community. We raise two points of criticism against current S‐LCA approaches. First, the development of S‐LCA methodology has not, to date, been based on experience with actual case studies. Second, for social impacts to be meaningfully assessed in a life cycle perspective, social indicators need to be unambiguously interpreted in all social contexts along the life cycle. We here discuss an empirically based approach to S‐LCA, illustrated by a case study of an automobile airbag system. The aim of the case study is to compare the injuries and lives lost during the product life cycle of the airbag system (excluding waste handling impacts) with the injuries prevented and lives saved during its use. The indicator used for assessing social impacts in this study is disability‐adjusted life years (DALY). The results from this study indicate that the purpose of an airbag system, which is to save lives and prevent injuries, is justified also in a life cycle perspective.     

Does Consideration and Assessment of Effects on Health Equity Affect the Conclusions of Systematic Reviews? A Methodology Study

Introduction

Tackling health inequities both within and between countries remains high on the agenda of international organizations including the World Health Organization and local, regional and national governments. Systematic reviews can be a useful tool to assess effects on equity in health status because they include studies conducted in a variety of settings and populations. This study aims to describe the extent to which the impacts of health interventions on equity in health status are considered in systematic reviews, describe methods used, and assess the implications of their equity related findings for policy, practice and research.

Methods

We conducted a methodology study of equity assessment in systematic reviews. Two independent reviewers extracted information on the reporting and analysis of impacts of health interventions on equity in health status in a group of 300 systematic reviews collected from all systematic reviews indexed in one month of MEDLINE, using a pre-tested data collection form. Any differences in data extraction were resolved by discussion.

Results

Of the 300 systematic reviews, 224 assessed the effectiveness of interventions on health outcomes. Of these 224 reviews, 29 systematic reviews assessed effects on equity in health status using subgroup analysis or targeted analyses of vulnerable populations. Of these, seven conducted subgroup analyses related to health equity which were reported in insufficient detail to judge their credibility. Of these 29 reviews, 18 described implications for policy and practice based on assessment of effects on health equity.

Conclusion

The quality and completeness of reporting should be enhanced as a priority, because without this policymakers and practitioners will continue lack the evidence base they need to inform decision-making about health inequity. Furthermore, there is a need to develop methods to systematically consider impacts on equity in health status that is currently lacking in systematic reviews.     

How Does Dog-Walking Influence Perceptions of Health and Wellbeing in Healthy Adults? A Qualitative Dog-Walk-Along Study

The aim of this study was to explore perceptions of health and wellbeing related to dog-walking in healthy adults. Ten self-reported healthy adult dog-walkers took part in one dog-walk-along interview, and nine of the 10 participants also attended one follow-up participatory analysis session. All interviews took place within the metropolitan area of Dunedin, Aotearoa/New Zealand. Dog-walk-along interviews were participant-led, audio and video recorded, and transcribed. Participants’ input into the inductive generation of themes was sought during the follow-up participatory analysis session. Three themes were identified: participants had an “Emotional Connection” with their dog(s); dog-walking led to “Healthy Interactions” (social and environmental interactions); and participants had come to value dog-walking for its aggregate benefits for Psychological Wellbeing. Our dog-walk-along interviews have revealed novel insights about how the emotional connection between human and dog strengthens intrinsic motivation to exercise (through dog-walking) whilst concurrently enhancing human psychological wellbeing through the act of giving something that gives pleasure to the dog. Through the combined methods of dog-walk-along interviews and participatory analysis sessions, we were able to identify negative influences on human psychological well-being that included anxiety about both dog behaviors and social encounters with other humans. Positive and negative aspects of dog-walking that we identified can be used by health promotion advocates to refine dog-walking related advice, programs, and initiatives.