共查询到20条相似文献,搜索用时 46 毫秒
1.
S. F. Perry C. J. Montpetit A. E. Julio K. Moore 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1999,169(4-5):335-343
The effect of long-term (7 day) anaemia on catecholamine release was examined in rainbow trout (Oncorhynchus mykiss) in vivo during acute exposure to hypoxia and in situ using a perfused post-cardinal vein preparation. The first goal was
to distinguish among reductions in blood O2 partial pressure, O2 concentration and haemoglobin percentage saturation as potential stimuli for, or correlates of, catecholamine secretion during
hypoxia. The second goal was to elucidate the role of these factors in promoting enhanced chromaffin cell responsiveness in
trout subjected to chronic hypoxia (Montpetit and Perry 1998). Anaemic fish (haematocrit lowered from 28.4±2.4% to 11.9±1.6%)
displayed a marked reduction in haemoglobin-O2 binding affinity [P
50 (P
aO2 at 50% Hb-O2 saturation) was increased from 14.7 mm Hg to 24.3 mm Hg]. Upon exposure to hypoxia, the anaemic fish released catecholamines
into their circulation at higher values of arterial O2 partial pressure (∼52 mm Hg versus ∼18 mm Hg) and haemoglobin O2 saturation (<70% versus <55%) than did control fish. In addition, anaemic fish achieved significantly greater circulating
levels of total catecholamines (noradrenaline plus adrenaline) during acute hypoxia (294.8±67.3 versus 107.0±35.6 nmol l−1). These results do not support the view that catecholamine release is triggered by a reduction in haemoglobin O2 saturation or arterial PO2, per se. Nor are they consistent with the idea that catecholamine release occurs at a threshold value of arterial PO2 corresponding to a critical reduction in blood O2 concentration. The effects of the non-selective cholinergic receptor carbachol on catecholamine secretion from chromaffin
tissue were assessed using perfused posterior cardinal vein preparations derived from control or anaemic fish. For adrenaline
secretion, there was no statistically significant change in the ED50 (dose eliciting 50% response). For noradrenaline secretion however, preparations originating from anaemic fish displayed
an enhanced responsiveness to carbachol as indicated by a significant 4.5-fold reduction in the carbachol ED50 value from 2.53 × 10−6 mol kg−1 to 5.67 × 10−7 mol kg−1. These results demonstrate that anaemia-induced hypoxaemia, in the absence of any lowering of PO2, is able to modulate the responsiveness of chromaffin cells to cholinergic stimulation.
Accepted: 21 April 1999 相似文献
2.
Juan Fuentes F. B. Eddy 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(6):438-443
Drinking in Atlantic salmon (Salmo salar) juveniles was investigated in fresh water and following transfer to sea water. There was a significant effect of fish size
on drinking, and smolts (20–30 g) imbibed about ten times less water than alevins of 0.2–0.3 g. Freshwater smolts drank at
a rate of 0.15 ± 0.03 ml · kg−1 · h−1 and administration of doses of 10 or 20 mg · kg−1 of papaverine (stimulator of the renin- angiotensin system RAS) or [Asn1, Val5]-Angiotensin II (0.4 μmol · kg−1) resulted in significant increases in drinking, while administration of the angiotensin converting enzyme inhibitor, enalapril
(50 mg · kg−1) had no effect on drinking. Transfer of Atlantic salmon smolts to 1/3, 2/3 and full strength sea water resulted in significant
increases in drinking to 1.06 ± 0.12, 1.24 ± 0.0.16 and 3.89 ± 0.28 ml · kg−1 · h−1, respectively. In sea water, stimulation of the endogenous RAS by administration of papaverine (20 mg · kg−1) resulted in a 20% increase in drinking, while administration of enalapril to doses of 50 and 200 mg · kg−1 lowered drinking to 1.99 ± 0.48 and 0.32 ± 0.06 ml · kg−1 · h−1, respectively. All treatments were without effect on blood plasma levels of Na+ and Cl− in fresh water, while in sea water smolts both stimulation and inhibition of drinking resulted in hemoconcentration of Na+ and Cl−. The role of the renin angiotensin system in control of drinking and hydromineral balance in Atlantic salmon is discussed.
Accepted: 27 February 1997 相似文献
3.
This study examined the thermoregulatory responses of men (group M) and women (group F) to uncompensable heat stress. In
total, 13 M [mean (SD) age 31.8 (4.7) years, mass 82.7 (12.5) kg, height␣1.79␣(0.06) m, surface area to mass ratio 2.46␣(0.18) m2 · kg−1 · 10−2, Dubois surface area 2.01 (0.16) m2, %body fatness 14.6 (3.9)%, V˙O2peak 49.0 (4.8) ml · kg−1 · min−1] and 17 F [23.2 (4.2) years, 62.4 (7.7) kg, 1.65 (0.07) m, 2.71 (0.14) m2 · kg−1 · 10−2, 1.68 (0.13) m2, 20.2 (4.8)%, 43.2 (6.6) ml · kg−1 · min−1, respectively] performed light intermittent exercise (repeated intervals of 15 min of walking at 4.0 km · h−1 followed by 15 min of seated rest) in the heat (40°C, 30% relative humidity) while wearing nuclear, biological, and chemical
protective clothing (0.29 m2 ·°C · W−1 or 1.88 clo, Woodcock vapour permeability coefficient 0.33 i
m). Group F consisted of eight non-users and nine users of oral contraceptives tested during the early follicular phase of
their menstrual cycle. Heart rates were higher for F throughout the session reaching 166.7 (15.9) beats · min−1 at 105 min (n = 13) compared with 145.1 (14.4) beats · min−1 for M. Sweat rates and evaporation rates from the clothing were lower and average skin temperature () was higher for F. The increase in rectal temperature (T
re) was significantly faster for the F, increasing 1.52 (0.29)°C after 105 min compared with an increase of 1.37 (0.29)°C for
M. Tolerance times were significantly longer for M [142.9 (24.5) min] than for F [119.3 (17.3) min]. Partitional calorimetric
estimates of heat storage (S) revealed that although the rate of S was similar between genders [42.1 (6.6) and 46.1 (9.7) W · m−2 for F and M, respectively], S expressed per unit of total mass was significantly lower for F [7.76 (1.44) kJ · kg−1] compared with M [9.45 (1.26) kJ · kg−1]. When subjects were matched for body fatness (n = 8 F and 8 M), tolerance times [124.5 (14.7) and 140.3 (27.4) min for F and M, respectively] and S [8.67 (1.44) and 9.39 (1.05) kJ · kg−1 for F and M, respectively] were not different between the genders. It was concluded that females are at a thermoregulatory
disadvantage compared with males when wearing protective clothing and exercising in a hot environment. This disadvantage can
be attributed to the lower specific heat of adipose versus non-adipose tissue and a higher percentage body fatness.
Accepted: 31 October 1997 相似文献
4.
Gerald S. Zavorsky David L. Montgomery David J. Pearsall 《European journal of applied physiology and occupational physiology》1998,77(3):224-230
The purposes of this study were to determine whether running economy (RE) is adversely affected following intense interval
bouts of 10 × 400-m running, and whether there is an interaction effect between RE and recovery duration during the workouts.
Twelve highly trained male endurance athletes [maximal oxygen consumption; V˙O2
max
=72.5 (4.3) ml·kg−1·min−1; mean (SD)] performed three interval running workouts of 10 × 400 m with a minimum of 4 days between runs. Recovery duration
between the repetitions was randomly assigned at 60, 120 or 180 s. The velocity for each 400-m run was determined from a treadmill
V˙O2
max
test. The average running velocity was 357.9 (9.0) m · min−1. Following the workout, the rating of perceived exertion (RPE) increased significantly (P < 0.01) as recovery duration between the 400-m repetitions decreased (14.4, 16.1, and 17.7 at 180s, 120s, and 60 s recovery,
respectively). Prior to and following each workout, RE was measured at speeds of 200 and 268 m · min−1. Changes in RE from pre- to post-workout, as well as heart rate (HR) and respiratory exchange ratio (R) were similar for the three recovery conditions. When averaged across conditions, oxygen consumption (V˙O2) increased significantly (P < 0.01) from pre- to post-test (from 38.5 to 40.5 ml · kg−1 · min−1 at 200 m · min−1, and from 53.1 to 54.5 ml · kg−1 · min−1 at 268 m · min−1, respectively). HR increased (from 124 to 138, and from 151 to 157 beats · min−1 respectively) and R decreased (from 0.90 to 0.78, and from 0.93 to 0.89, respectively) at 200 and 268 m · min−1, respectively (P < 0.01). This study showed that RE can be perturbed after a high-intensity interval workout and that the changes in V˙O2, HR and R were independent of the recovery duration between the repetitions.
Accepted: 23 June 1997 相似文献
5.
D. G. Butler R. Zandevakili G. Y. Oudit 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1998,168(3):213-224
The vertebrate renin-angiotensin system controls cardiovascular, renal and osmoregulatory functions. Angiotensin II (ANG
II) is the most potent hormone of the RAS but in some vertebrate animals angiotensin III (Val4-ANG III) may be a hormone. We studied the effects of some angiotensins and mammalian ANG II receptor antagonists on nasal
salt gland function and arterial blood pressure in conscious white Pekin ducks. Nasal salt gland fluid secretion (NFS) was
induced by a 10 ml · kg−1 bw i.v. injection of a NaCl solution (1000 mosmol · kg−1 H2O) and maintained by a continuous i.v. infusion of the same solution at a rate of 0.97 ml · min−1. There was a positive linear correlation between nasal fluid [Na+] and osmolality, between [Na+] and [K+], and also between the rate of NFS and [Na+] and [K+]. [Asp1,Val5]-ANG II (1 nmol · kg−1 i.v.) inhibited NFS but did not change ionic concentrations. Val4-ANG III (1 or 5 nmol · kg−1) and ANG I (1-7) (20 nmol · kg−1) had no effect on NFS. [Sar1, Ile8]-ANG II (SARILE) acted as an ANG II receptor agonist and resulted in a prolonged and complete inhibition of NFS. The AT1 receptor antagonist, losartan (DuP 753) and the AT2 receptor antagonist, PD 123319 both failed to block the inhibitory effect of [Asp1, Val5]-ANG II on the nasal salt glands.
[Asp1,Val5]-ANG II (2 nmol · kg−1 i.v.) increased mean arterial blood pressure (MABP), whereas the same dose of [Asn1,Val5]-ANG II (teleost) had only 30% of the pressor potency of the avian ANG II. Neither 1 nor 5 nmol · kg−1 of Val4-ANG III i.v. nor 20 nmol · kg−1 of ANG I (1-7) had any measurable effect on MABP. SARILE blocked completely the pressor response to [Asp1,Val5]-ANG II but the AT1 antagonists losartan and CGP 48933 and the AT2 antagonist PD 123319 all failed to block the pressor response to [Asp1,Val5]-ANG II. These results have substantiated an important role of the nasal salt gland in potassium regulation and highlighted
a pharmacological dimorphism of saralasin, namely agonist and antagonist to angiotensin II-mediated inhibition of nasal salt
gland function and pressor response, respectively. Using specific nonpeptidergic angiotensin II receptor antagonists, we have
confirmed the distinct pharmacology of the avian angiotensin II receptors in a nongallinaceous species and the absence of
significant angiotensin I (1-7) and angiotensin II effects on the cardiovascular system and nasal salt gland.
Accepted: 6 November 1997 相似文献
6.
R. Candau A. Belli G. Y. Millet D. Georges B. Barbier J. D. Rouillon 《European journal of applied physiology and occupational physiology》1998,77(6):479-485
The aim of the present study was to examine the physiological and mechanical factors which may be concerned in the increase
in energy cost during running in a fatigued state. A group of 15 trained triathletes ran on a treadmill at velocities corresponding
to their personal records over 3000m [mean 4.53 (SD 0.28) m · s−1] until they felt exhausted. The energy cost of running (C
R) was quantified from the net O2 uptake and the elevation of blood lactate concentration. Gas exchange was measured over 1 min firstly during the 3rd–4th min
and secondly during the last minute of the run. Blood samples were collected before and after the completion of the run. Mechanical
changes of the centre of mass were quantified using a kinematic arm. A significant mean increase [6.9 (SD 3.5)%, P < 0.001] in C
R from a mean of 4.4 (SD 0.4) J · kg−1 · m−1 to a mean of 4.7 (SD 0.4) J · kg−1 · m−1 was observed. The increase in the O2 demand of the respiratory muscles estimated from the increase in ventilation accounted for a considerable proportion [mean
25.2 (SD 10.4)%] of the increase in CR. A mean increase [17.0 (SD 26.0)%, P < 0.05] in the mechanical cost (C
M) from a mean of 2.36 (SD 0.23) J · kg−1 · m−1 to a mean of 2.74 (SD 0.55) J · kg−1 · m−1 was also noted. A significant correlation was found between C
R and C
M in the non-fatigued state (r = 0.68, P < 0.01), but not in the fatigued state (r = 0.25, NS). Furthermore, no correlations were found between the changes (from non-fatigued to fatigued state) in C
R and the changes in C
M suggesting that the increase in C
R is not solely dependent on the external work done per unit of distance. Since step frequency decreased slightly in the fatigued
state, the internal work would have tended to decrease slightly which would not be compatible with an increase in C
R. A stepwise regressions showed that the changes in C
R were linked (r = 0.77, P < 0.01) to the changes in the variability of step frequency and in the variability of potential cost suggesting that a large
proportion of the increase in C
R was due to an increase in the step variability. The underlying mechanisms of the relationship between C
R and step variability remains unclear.
Accepted: 15 September 1997 相似文献
7.
For the first time, the purification has been achieved of the type II polyhydroxyalkanoate (PHA) synthases PhaC1 and PhaC2
from Pseudomonas aeruginosa applying N-terminal His6-tag fusions and metal chelate affinity chromatography. In vivo His6-tagged PHA synthase activity was confirmed by functional expression of the corresponding genes in Escherichia coli, and PHA synthase activity could also be measured in vitro with the enzymes. The specific enzyme activity of PHA synthases PhaC1 and PhaC2 was 0.039 U mg−1 and 0.035 U mg−1 protein, respectively. Kinetic studies showed a lag phase for both PHA synthases using (R,S)-3-hydroxydecanoyl-CoA as substrate. Specific enzyme activity was increased to 0.055 U mg−1 when the phasin GA24 from Ralstonia eutropha was added to the assay. CoA inhibited PHA synthase activity, and a K
i of 85 μM was determined. A two-enzyme system was established, employing commercially available acyl-CoA synthetase and PHA
synthase, which allowed the in vitro de novo PHA granule formation and the in vitro synthesis of poly(3-hydroxydecanoate) exhibiting a weight average molar mass of 9.8 × 104 g mol−1, and which occurred independently of pre-existing PHA granules.
Received: 3 December 1999 / Revision received: 10 January 2000 / Accepted: 14 January 2000 相似文献
8.
H. Arabi H. Vandewalle P. Pitor J. de Lattre H. Monod 《European journal of applied physiology and occupational physiology》1997,76(2):122-127
The present experiment was designed to study the importance of strength and muscle mass as factors limiting maximal oxygen
uptake (V˙O2
max
) in wheelchair subjects. Thirteen paraplegic subjects [mean age 29.8 (8.7) years] were studied during continuous incremental
exercises until exhaustion on an arm-cranking ergometer (AC), a wheelchair ergometer (WE) and motor-driven treadmill (TM).
Lean arm volume (LAV) was estimated using an anthropometric method based upon the measurement of various circumferences of
the arm and forearm. Maximal strength (MVF) was measured while pushing on the rim of the wheelchair for three positions of
the hand on the rim (−30°, 0° and +30°). The results indicate that paraplegic subjects reached a similar V˙O2
max
[1.23 (0.34) l · min−1, 1.25 (0.38) l · min−1, 1.22 (0.18) l · min−1 for AC, TM and WE, respectively] and V˙O2
max
/body mass [19.7 (5.2) ml · min−1 · kg−1, 19.5 (6.14) ml · min−1 · kg−1, 19.18 (4.27) ml · min−1 · kg−1 for AC, TM and WE, respectively on the three ergometers. Maximal heart rate f
c
max
during the last minute of AC (173 (17) beats · min−1], TM [168 (14) beats · min−1], and WE [165 (16) beats · min−1], were correlated, but f
c
max
was significantly higher for AC than for TM (P<0.03). There were significant correlations between MVF and LAV (P<0.001) and between the MVF data obtained at different angles of the hand on the rim [311.9 (90.1) N, 313.2 (81.2) N, 257.1
(71) N, at −30°, 0° and +30°, respectively]. There was no correlation between V˙O2
max
and LAV or MVF. The relatively low values of f
c
max
suggest that V˙O2
max
was, at least in part, limited by local aerobic factors instead of central cardiovascular factors. On the other hand, the
lack of a significant correlation between V˙O2
max
and MVF or muscle mass was not in favour of muscle strength being the main factor limiting V˙O2
max
in our subjects.
Accepted: 31 January 1997 相似文献
9.
D. A. Gray S. K. Maloney 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(8):558-562
The relationship between body temperature (T
b) and the plasma concentrations of arginine vasotocin (AVT) and angiotensin II (AII) was examined in conscious, adult Pekin
ducks. Exposure of birds to an ambient temperature of 40 °C for 3 h increased T
b by about 1.5 °C and increased breathing rate five-fold. Plasma osmolality was elevated from the normothermic value of 294.9 ± 1.4
mosmol kg−1 by about 8 mosmol kg−1 Circulating AVT levels increased by about 2 pg ml−1 from a basal concentration of 4.98 ± 0.15 pg ml−1, a rise which could be accounted for by the change in osmotic status. Plasma AII concentrations were unchanged from the pre-heat
exposure value of 31.8 ± 3.4 pg ml−1. Time control birds, exposed only to an ambient temperature of 22 °C demonstrated no significant changes in any of the measured
variables. The results suggest that an increased T
b has no direct effect on the circulating concentrations of AVT or AII in ducks.
Accepted: 2 June 1997 相似文献
10.
Nigel J. Adams Berry Pinshow Leonard Z. Gannes 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1997,167(6):444-450
We used tritium-labeled water to measure total body water, water influx (which approximated oxidative water production) and
water efflux in free-flying tippler pigeons (Columba livia) during flights that lasted on average 4.2 h. At experimental air temperatures ranging from 18 to 27 °C, mean water efflux
by evaporation and excretion [6.3 ± 1.3 (SD) ml · h−1, n = 14] exceeded water influx from oxidative water and inspired air (1.4 ± 0.7 ml · h−1, n = 14), and the birds dehydrated at 4.9 ± 0.9 ml · h−1. This was not significantly different from gravimetrically measured mass loss of 6.2 ± 2.1 g · h−1 (t = 1.902, n = 14, P>0.05). This flight-induced dehydration resulted in an increase in plasma osmolality of 4.3 ± 3.0 mosmol · kg−1 · h−1 during flights of 3–4 h. At 27 °C, the increase in plasma osmolality above pre-flight levels (ΔP
osm = 7.6±4.29 mosmol · kg−1 · h−1, n = 6) was significantly higher than that at 18 °C (ΔP
osm = 0.83±2.23 mosmol · kg−1 · h−1, (t = 3.43, n = 6, P < 0.05). Post-flight haematocrit values were on average 1.1% lower than pre-flight levels, suggesting plasma expansion. Water
efflux values during free flight were within 9% of those in the one published field study (Gessaman et al. 1991), and within
the range of values for net water loss determined from mass balance during wind tunnel experiments (Biesel and Nachtigall
1987). Our net water loss rates were substantially higher than those estimated by a simulation model (Carmi et al. 1992) suggesting
some re-evaluation of the model assumptions is required.
Accepted: 8 April 1997 相似文献
11.
Aldegunde M Andrés MD Soengas JL 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2000,170(3):237-243
The influx of glucose into the brain and plasma glucose disappearance were estimated in rainbow trout (Oncorhynchus mykiss) intravenously injected (1 ml · kg−1 body weight) with a single dose (15 μCi · kg−1 body weight) of 3-O-methyl-D-[U-14C]glucose ([U-14C]-3-OMG) at different times (2–160 min), and after intravenous injection at 15 min of increased doses (10–60 μCi · kg−1 body weight) of [U-14C]-3-OMG. Brain and plasma radiotracer concentrations were measured, and several kinetic parameters were calculated. The apparent
brain glucose influx showed a maximum after 15–20 min of injection then decreased to a plateau after 80 min. Brain distribution
space of 3-OMG increased from 2 min to 20 min reaching equilibrium from that time onwards at a value of 0.14 ml · g−1. The unidirectional clearance of glucose from blood to brain (k1) and the fractional clearance of glucose from brain to blood (k2) were estimated to be 0.093 ml · min−1 · g−1, and 0.867 min−1, respectively. A linear increase was observed in brain and plasma radiotracer concentrations when increased doses of [U-14C]-3-OMG were used. All these findings support a facilitative transport of glucose through the blood-brain barrier of rainbow
trout with characteristics similar to those observed in mammals. The injection of different doses of melatonin (0.25–1.0 mg · kg−1) significantly increased brain glucose influx suggesting a possible role for melatonin in the regulation of glucose transport
into the brain.
Accepted: 26 January 2000 相似文献
12.
Ronald Heus Alexander H. Wertheim George Havenith 《European journal of applied physiology and occupational physiology》1998,77(4):388-394
The assumption that working on board ship is more strenuous than comparable work ashore was investigated in this study. Various
physiological parameters (V˙O2, V˙CO2, V˙
E and HR) have been measured to determine the energy expenditure of subjects walking slowly on a moving platform (ship motion
simulator). Twelve subjects (eight men and four women) walked either freely on the floor or on a treadmill at a speed of 1 m · s−1. Platform motion was either in a heave, pitch or roll mode. These three conditions were compared with a control condition
in which the platform remained stationary. The results showed that during pitch and roll movements of the platform, the energy
expenditure for the same walking task was about 30% higher than under the stationary control condition (3.6 J · kg−1 · m−1 vs 2.5 J · kg−1 · m−1, P < 0.05) for both walking on a treadmill and free walking. The heart rate data supported the higher energy expenditure results
with an elevation of the heart rate (112 beats · min−1 vs 103 beats · min−1, P < 0.05). The heave condition did not differ significantly from the stationary control condition. Pitch and roll were not
significantly different from each other. During all experimental conditions free walking resulted in a higher energy cost
of walking than treadmill walking (3.5 J · kg−1 · m−1 vs 2.7 J · kg−1 · m−1, P < 0.05) at the same average speed. The results of this experiment were interpreted as indicating that the muscular effort,
needed for maintaining balance when walking on a pitching or rolling platform, resulted in a significantly higher work load
than similar walking on a stable or a heaving floor, independent of the mode of walking. These results explain in part the
increased fatigue observed when a task is performed on a moving platform.
Accepted: 3 October 1997 相似文献
13.
Autotrophic and heterotrophic respiration in needle fir and Quercus-dominated stands in a cool-temperate forest, central Korea 总被引:3,自引:0,他引:3
Na-yeon Lee Jin-Woo Koo Nam Jin Noh Joon Kim Yowhan Son 《Journal of plant research》2010,123(4):485-495
To investigate annual variation in soil respiration (R
S) and its components [autotrophic (R
A) and heterotrophic (R
H)] in relation to seasonal changes in soil temperature (ST) and soil water content (SWC) in an Abies holophylla stand (stand A) and a Quercus-dominated stand (stand Q), we set up trenched plots and measured R
S, ST and SWC for 2 years. The mean annual rate of R
S was 436 mg CO2 m−2 h−1, ranging from 76 to 1,170 mg CO2 m−2 h−1, in stand A and 376 mg CO2 m−2 h−1, ranging from 82 to 1,133 mg CO2 m−2 h−1, in stand Q. A significant relationship between R
S and its components and ST was observed over the 2 years in both stands, whereas a significant correlation between R
A and SWC was detected only in stand Q. On average over the 2 years, R
A accounted for approximately 34% (range 17–67%) and 31% (15–82%) of the variation in R
S in stands A and Q, respectively. Our results suggested that vegetation type did not significantly affect the annual mean
contributions of R
A or R
H, but did affect the pattern of seasonal change in the contribution of R
A to R
S. 相似文献
14.
A. Pires S. L. Coon M. G. Hadfield 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1997,181(3):187-194
The content of catecholamines and dihydroxyphenylalanine in larvae of the nudibranch Phestilla sibogae was analyzed by high-performance liquid chromatography with electrochemical detection. Dihydroxyphenylalanine, norepinephrine
and dopamine were identified in larvae of all ages examined (5 through 12 days post-fertilization). Dihydroxyphenylalanine
could be accurately quantified only in larvae of ages 8 through 12 days, when its average concentration increased from 0.62
to 6.71 × 10−2 pmol μg protein−1. Between ages 5 and 12 days dopamine rose from 0.081 to 0.616 pmol μg protein−1, and norepinephrine from 0.45 to 2.17 × 10−2 pmol μg protein−1. Dihydroxyphenylalanine, dopamine and norepinephrine were also measured at different stages of metamorphic progress in 10-
to 12-day larvae. Dihydroxyphenylalanine increased by a factor of 2.4 between the onset and completion of metamorphosis, but
levels of dopamine and norepinephrine remained stable. One millimolar alpha-methyl-dl-m-tyrosine, an inhibitor of catecholamine synthesis, inhibited natural metamorphosis and depleted endogenous norepinephrine
and especially dopamine, respectively, to 75% and 35% of control values. The existence of unexpectedly high levels of catecholamines
in metamorphically competent larvae, and the association of catecholamine depletion with inhibition of metamorphosis, indicate
that these compounds may participate in the control of gastropod development.
Accepted: 18 April 1997 相似文献
15.
Greg J. Wilson Andrew D. Walshe Mark R. Fisher 《European journal of applied physiology and occupational physiology》1997,75(5):455-461
The aims of the present study were: (1) to assess aerobic metabolism in paraplegic (P) athletes (spinal lesion level, T4–L3)
by means of peak oxygen uptake (V˙O2peak) and ventilatory threshold (VT), and (2) to determine the nature of exercise limitation in these athletes by means of cardioventilatory
responses at peak exercise. Eight P athletes underwent conventional spirographic measurements and then performed an incremental
wheelchair exercise on an adapted treadmill. Ventilatory data were collected every minute using an automated metabolic system:
ventilation (l · min−1), oxygen uptake (V˙O2, l · min−1, ml · min−1 · kg−1), carbon dioxide production (V˙CO2, ml · min−1), respiratory exchange ratio, breathing frequency and tidal volume. Heart rate (HR, beats · min−1) was collected with the aid of a standard electrocardiogram. V˙O2peak was determined using conventional criteria. VT was determined by the breakpoint in the V˙CO2−V˙O2 relationship, and is expressed as the absolute VT (V˙O2, ml · min−1 · kg−1) and relative VT (percentage of V˙O2peak). Spirometric values and cardioventilatory responses at rest and at peak exercise allowed the measurement of ventilatory
reserve (VR), heart rate reserve (HRr), heart rate response (HRR), and O2 pulse (O2 P). Results showed a V˙O2peak value of 40.6 (2.5) ml · min−1 · kg−1, an absolute VT detected at 23.1 (1.5) ml · min−1 · kg−1
V˙O2 and a relative VT at 56.4 (2.2)% V˙O2peak. HRr [15.8 (3.2) beats · min−1], HRR [48.6 (4.3) beat · l−1], and O2 P [0.23 (0.02) ml · kg−1 · beat−1] were normal, whereas VR at peak exercise [42.7 (2.4)%] was increased. As wheelchair exercise excluded the use of an able-bodied
(AB) control group, we compared our V˙O2peak and VT results with those for other P subjects and AB controls reported in the literature, and we compared our cardioventilatory
responses with those for respiratory and cardiac patients. The low V˙O2peak values obtained compared with subject values obtained during an arm-crank exercise may be due to a reduced active muscle
mass. Absolute VT was somewhat comparable to that of AB subjects, mainly due to the similar muscle mass involved in wheelchair
and arm-crank exercise by P and AB subjects, respectively. The increased VR, as reported in patients with chronic heart failure,
suggested that P athletes exhibited cardiac limitation at peak exercise, and this contributed to the lower V˙O2peak measured in these subjects.
Accepted: 22 April 1997 相似文献
16.
D. P. Heil T. R. Derrick S. Whittlesey 《European journal of applied physiology and occupational physiology》1997,75(2):160-165
This study was designed to determine how changes in oxygen uptake (O2) and heart rate (HR) during submaximal cycle ergometry were determined by changes in cycle geometry and/or lower-limb kinematics.
Fourteen trained cyclists [Mean (SD): age, 25.5 (6.4) years; body mass 74.4 (8.8) kg; peak O2, 4.76 (0.79) l. min−1 peak] were tested at three seat-tube angles (70°, 80°, 90°) at each of three trunk angles (10°, 20°, 30°) using a modified
Monark cycle ergometer. All conditions were tested at a power output corresponding to 95% of the O2 at each subject's ventilatory threshold while pedalling at 90 rpm and using aerodynamic handlebars. Sagittal-view kinematics
for the hip, knee, and ankle joints were also recorded for all conditions and for the subjects' preferred positioning on their
own bicycles. No combination of seat-tube and trunk angle could be considered optimal since many of the nine conditions elicited
statistically similar mean O2 and HR values. Mean hip angle (HA) was the only kinematic variable that changed consistently across conditions. A regression
relationship was not observed between mean O2 or HR and mean hip angle values (P > 0.45). Significant curvilinear relationships were observed, however, between ΔO2 (O2 − minimum O2) and ΔHA (mean HA − preferred HA) using the data from all subjects (R = 0.45, SEE = 0.13 l . min−1) and using group mean values (R = 0.93, SEE = 0.03 l . min−1). In both cases ΔO2 minimized at ΔHA = 0, which corresponded to the subjects' preferred HA from their own bicycles. Thus, subjects optimized
their O2 cost at cycle geometries that elicited similar lower-limb kinematics as the preferred geometries from their own bicycles.
Accepted: 3 July 1996 相似文献
17.
C. E. Crocker J. J. Cech Jr. 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1998,168(1):50-60
The effect of environmental hypercapnia on respiratory and acid-base variables was studied in white sturgeon, Acipenser transmontanus. Blood PCO2, PO2, pH, hemoglobin concentration, and plasma lactate, glucose, catecholamines and cortisol were measured first under normocapnia
(water PCO2 < 0.5 Torr, 1 Torr = 133.32 Pa), then under hypercapnia (25–35 Torr) and a final return to normocapnia at 19 ± 0.5 °C. Acute
(≤ 2h) hypercapnia significantly increased arterial PCO2 (8-fold increase), ventilation frequency (2-fold increase), plasma HCO3
− (2.3-fold) and decreased arterial pH (to 7.15 ± 0.02). After 24 h, norepinephrine, epinephrine and cortisol, were significantly
increased, and arterial pH reached its nadir (7.10 ± 0.03). During the 72- and 96-h-periods, arterial PCO2 (24 ± 4.4 Torr) and ventilatory frequency (105 ± 5 breaths min−1) stabilized, HCO3
− reached its apparent maximum (23.6 ± 0.0 mmol−1), glucose decreased by 32%, and pH increased significantly to 7.31 + 0.03. The return to normocapnia completely restored
arterial PCO2 (2.5 ± 0.14 Torr), HCO3
− (7.4 ± 0.59 mmol · l−1), ventilation frequency (71 ± 7 breaths · min−1), and pH (7.75 ± 0.04). Overall, hypercapnia produced a respiratory acidosis, hyperventilation, a transient norepinephrine
“spike”, and increased plasma catecholamines, cortisol, and arterial PO2. The respiratory acidosis was only partially compensated (35% pH restoration) 96 h after the onset of hypercapnia and resulted
in a significantly decreased blood-O2 affinity (Bohr effect), as determined by construction of in vitro blood O2 equilibrium curves at 15 °C and 20 °C. Prolonged exposure to hypercapnia may lead to acid-base disturbances and negatively
affect growth of white sturgeon.
Accepted: 17 August 1997 相似文献
18.
Delorme M Thomas DW 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1999,169(2):123-132
Nitrogen (N) and energy (E) requirements of the phyllostomid fruit bat, Artibeus jamaicensis, and the pteropodid fruit bat Rousettus aegyptiacus, were measured in adults that were fed on four experimental diets. Mean daily food intake by A. jamaicensis and R. aegyptiacus ranged from 1.1–1.6 times body mass and 0.8–1.0 times body mass, respectively. Dry matter digestibility and metabolizable
E coefficient were high (81.1% and 82.4%, respectively) for A. jamaicensis and (77.5% and 78.0%, respectively) for R. aegyptiacus. Across the four diets, bats maintained constant body mass with mean metabolizable E intakes ranging from 1357.3 kJ · kg−0.75 · day−1 to 1767.3 kJ · kg−0.75 · day−1 for A. jamaicensis and 1282.6–1545.2 kJ · kg−0.75 · day−1 for R. aegyptiacus. Maintenance E costs were high, in the order of 3.6–5.4 times the basal metabolic rate (BMR). It is unlikely that the E intakes
that we observed represent a true measure of maintenance E requirements. All evidence seems to indicate that fruit bats are
E maximizers, ingesting more E than required and regulating storage by adjusting metabolic output. We suggest that true maintenance
E requirements are substantially lower than what we observed. If it follows the eutherian norm of two times the BMR, fruit
bats must necessarily over-ingest E on low-N fruit diet. Dietary E content did affect N metabolism of A. jamaicensis. On respective low- and high-E diets, metabolic fecal N were 0.492 mg N · g−1 and 0.756 mg N · g−1 dry matter intake and endogenous urinary N losses were 163.31 mg N · kg−0.75 · day−1 and 71.54 mg N · kg−0.75 · day−1. A. jamaicensis required 332.3 mg · kg−0.75 · day−1 and 885.3 mg · kg−0.75 · day−1 of total N on high- and low-E diets, respectively, and 213.7 mg · kg−0.75 · day−1 of truly digestible N to achieve N balance. True N digestibilities were low (29% and 49%) for low- and high-E diets, respectively.
For R. aegyptiacus, metabolic fecal N and endogenous urinary N losses were 1.27 mg N · g−1 dry matter intake and 96.0 mg N · kg−0.75 · day−1, respectively, and bats required 529.8 mg · kg−0.75 · day−1 (total N) or 284.0 mg · kg−0.75 · day−1 (truly digestible N). True N digestibility was relatively low (50%). Based on direct comparison, we found no evidence that
R. aegyptiacus exhibits a greater degree of specialization in digestive function and N retention than A. jamaicensis. When combined with results from previous studies, our results indicate that all fruit bats appear to be specialized in their
ability to retain N when faced with low N diet.
Accepted: 24 November 1998 相似文献
19.
Tree species and wood ash application in plantations of short-rotation woody crops (SRWC) may have important effects on the
soil productive capacity through their influence on soil organic matter (SOM) and exchangeable cations. An experiment was
conducted to assess changes in soil C and N contents and pH within the 0–50 cm depth, and exchangeable cation (Ca2+, Mg2+, K+, and Na+) and extractable acidity concentrations within the 0–10 cm depth. The effects of different species (European larch [Larix decidua P. Mill.], aspen [Populus tremula L. × Populus tremuloides Michx.], and four poplar [Populus spp.] clones) and wood ash applications (0, 9, and 18 Mg ha−1) on soil properties were evaluated, using a common garden experiment (N = 70 stands) over 7 years of management in Michigan’s Upper Peninsula. Soils were of the Onaway series (fine-loamy, mixed,
active, frigid Inceptic Hapludalfs). The NM-6 poplar clone had the greatest soil C and N contents in almost all ash treatment
levels. Soil C contents were 7.5, 19.4, and 10.7 Mg C ha−1 greater under the NM-6 poplar than under larch in the ash-free, medium-, and high-level plots, respectively. Within the surface
layer, ash application increased soil C and N contents (P < 0.05) through the addition of about 0.7 Mg C ha−1 and 3 kg N ha−1 with the 9 Mg ha−1 ash application (twofold greater C and N amounts were added with the 18 Mg ha−1 application). During a decadal time scale, tree species had no effects—except for K+—on the concentrations of the exchangeable cations, pH, and extractable acidity. In contrast, ash application increased soil
pH and the concentration of Ca2+ (P < 0.05), from 5.2 ± 0.4 cmolc kg−1 (ash-free plots) to 8.6 ± 0.4 cmolc kg−1 (high-level ash plots), and tended to increase the concentration of Mg2+ (P < 0.1), while extractable acidity was reduced (P < 0.05) from 5.6 ± 0.2 cmolc kg−1 (ash-free plots) to 3.7 ± 0.2 cmolc kg−1 (high-level plots). Wood ash application, within certain limits, not only had a beneficial effect on soil properties important
to the long-term productivity of fast-growing plantations but also enhanced long-term soil C sequestration. 相似文献
20.
Carlo Capelli Dave R. Pendergast B. Termin 《European journal of applied physiology and occupational physiology》1998,78(5):385-393
The energy cost per unit of distance (C
s, kilojoules per metre) of the front-crawl, back, breast and butterfly strokes was assessed in 20 elite swimmers. At sub-maximal
speeds (v), C
s was measured dividing steady-state oxygen consumption (V˙O2) by the speed (v, metres per second). At supra-maximal v, C
s was calculated by dividing the total metabolic energy (E, kilojoules) spent in covering 45.7, 91.4 and 182.9 m by the distance. E was obtained as: E = E
an+V˙O2max
t
p−V˙O2max(1−e−(
t
p/)), where E
an was the amount of energy (kilojoules) derived from anaerobic sources, V˙O2max litres per second was the maximal oxygen uptake, α (=20.9 kJ · l O2
−1) was the energy equivalent of O2, τ (24 s) was the time constant assumed for the attainment of V˙O2max at muscle level at the onset of exercise, and t
p (seconds) was the performance time. The lactic acid component was assumed to increase exponentially with t
p to an asymptotic value of 0.418 kJ · kg−1 of body mass for t
p ≥ 120 s. The lactic acid component of E
an was obtained from the net increase of lactate concentration after exercise (Δ[La]b) assuming that, when Δ[La]b = 1 mmol · l−1 the net amount of metabolic energy released by lactate formation was 0.069 kJ · kg−1. Over the entire range of v, front crawl was the least costly stroke. For example at 1 m · s−1, C
s amounted, on average, to 0.70, 0.84, 0.82 and 0.124 kJ · m−1 in front crawl, backstroke, butterfly and breaststroke, respectively; at 1.5 m · s−1, C
s was 1.23, 1.47, 1.55 and 1.87 kJ · m−1 in the four strokes, respectively. The C
s was a continuous function of the speed in all of the four strokes. It increased exponentially in crawl and backstroke, whereas
in butterfly C
s attained a minimum at the two lowest v to increase exponentially at higher v. The C
s in breaststroke was a linear function of the v, probably because of the considerable amount of energy spent in this stroke for accelerating the body during the pushing
phase so as to compensate for the loss of v occurring in the non-propulsive phase.
Accepted: 14 April 1998 相似文献