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1.
Armelle Baeza-Squiban Sylvie Romet Anne Moreau Francelyne Marano 《In vitro cellular & developmental biology. Animal》1991,27(6):453-460
Summary Primary cultures of rabbit tracheal cells were obtained as outgrowths from explants of tracheal mucosa. A 30% collagen substratum
containing serum and minimal essential medium was required for obtaining an outgrowth of epithelial cells keeping their differentiated
characteristics. The tracheal epithelial cells obtained near the explant in the first days of culture presented morphologic
similarities with normal tracheal epithelium. Cultures contained basal cells and epithelial polarized cells that exhibited
apical tight junctions and desmosomes. Ciliated cells stayed functional during all time culture. Their number slightly increased
at the beginning of the culture and then stayed constant when the total number of cells increased. Development of the outgrowth
was rapid and significant inasmuch as the outgrowth surface reached 30 times that of the explant after less than 8 days. This
was linked to cellular proliferation, as demonstrated by the incorporation of bromodeoxyuridine (BrdU) in phase-S nuclei and
the revelation of BrdU using an immunofluorescence technique. The epithelial nature of the outgrowth cells and the absence
of contamination with fibroblasts were established by positive staining with anti-keratin antibody and by negative staining
with anti-vimentin antibody, respectively.
This work was supported by DRET and by CIFRE grant awarded to S. R. 相似文献
2.
Human ovarian surface epithelium in primary culture 总被引:6,自引:0,他引:6
The ovarian surface epithelium (OSE) represents a minute fraction of the cell mass of the ovary but gives rise to over 80% of human ovarian carcinomas. No experimental models for the study of human OSE exist. To characterize OSE cells in culture, explants of ovarian surface from normal ovary of premenopausal women were grown on plastic, glass, and collagen gel in 25% fetal bovine serum/Waymouth's medium 752/1. About 25% of explants produced epithelial outgrowths. Morphologically, these outgrowths resembled OSE in vivo and endothelial and mesothelial cells in culture, but they differed from cultured ovarian stromal, granulosa, and luteal cells. Only OSE among ovarian cell types were intensely keratin positive by immunofluorescence. Keratin also distinguished OSE cells from the keratin-negative endothelial cells. Most but not all OSE colonies tested showed 17 beta-hydroxysteroid dehydrogenase (HSD) activity, which was absent in peritoneal mesothelial cells. Colonies from most patients were limited to a few millimetres and became stationary within a few weeks. Changes that accompanied cessation of growth included senescence, increased keratin content, or the formation of multicellular papillary aggregates. With time, OSE cells tended to assume a fibroblast-like morphology but remained keratin positive and continued to resemble OSE by scanning electron microscopy (SEM). Subcultured OSE cells persisted in a stationary keratin-positive form for many weeks. Throughout this study, all pavementlike epithelial outgrowths that were contiguous with an explant stained for keratin; thus, such colonies can be assumed to be OSE. Conversely, fibroblast-shaped cells may represent OSE as indicated by keratin content and SEM appearance. The methods presented here permit culture of normal human OSE under conditions in which the cells exhibit morphologic plasticity, variable 17 beta-HSD activity, and presence of keratin. 相似文献
3.
Floyd M. Price Richard F. Camalier Raymond Gantt William G. Taylor Gilbert H. Smith Katherine K. Sanford 《In vitro cellular & developmental biology. Plant》1980,16(2):147-158
Summary A new culture medium, NCTC 168, has been designed for human skin epithelial cells. This medium formulation was developed,
by combining and testing at various concentrations, components of media NCTC 135 and 163, since a 1∶1 mixture of these two
media with 10% horse serum supplement was found to promote epithelial cell outgrowth from human skin explants. The buffer
system in NCTC 168 maintains the pH of the medium between 7.0 and 7.2. In contrast to other media tested, NCTC 168 with 10%
horse serum is capable of initiating and sustaining larger epithelial cell outgrowths. Explants in serum-supplemented NCTC
168 in the absence of feeder cells reproducibly yield confluent epithelial cell sheets apparently free of fibroblasts after
only 19 to 28 days as compared with 5 weeks or longer for the other media tested. NCTC 168 also supports passage of human
epithelial cells to the sixth subculture generation without feeder cells. Electron microscopy has shown the presence of desmosomes
and tonofilaments in the passaged cells indicating the epithelial nature of the cells. The addition of epithelial growth factor,
hydrocortisone and insulin at 5 ng per ml, 4 μg per ml and 5 μg per ml, respectively did not appreciably enhance the growth
of the epithelial cells. 相似文献
4.
N. Auersperg C. H. Siemens S. E. Myrdal 《In vitro cellular & developmental biology. Plant》1984,20(10):743-755
Summary The ovarian surface epithelium (OSE) represents a minute fraction of the cell mass of the ovary but gives rise to over 80%
of human ovarian carcinomas. No experimental models for the study of human OSE exist. To characterize OSE cells in culture,
explants of ovarian surface from normal ovary of premenopausal women were grown on plastic, glass, and collagen gel in 25%
fetal bovine serum/Waymouth's medium 752/1. About 25% of explants produced epithelial outgrowths. Morphologically, these outgrowths
resembled OSE in vivo and endothelial and mesothelial cells in culture, but they differed from cultured ovarian stromal, granulosa,
and luteal cells. Only OSE among ovarian cell types were intensely keratin positive by immunofluorescence. Keratin also distinguished
OSE cells from the keratin-negative endothelial cells. Most but not all OSE colonies tested showed 17β-hydroxysteroid dehydrogenase
(HSD) activity, which was absent in peritoneal mesothelial cells. Colonies from most patients were limited to a few millimetres
and became stationary within a few weeks. Changes that accompanied cessation of growth included senescence, increased keratin
content, or the formation of multicellular papillary aggregates. With time, OSE cells tended to assume a fibroblast-like morphology
but remained keratin positive and continued to resemble OSE by scanning electron microscopy (SEM). Subcultured OSE cells persisted
in a stationary keratin-positive form for many weeks. Throughout this study, all pavementlike epithelial outgrowths that were
contiguous with an explant stained for keratin; thus, such colonies can be assumed to be OSE. Conversely, fibroblast-shaped
cells may represent OSE as indicated by keratin content and SEM appearance. The methods presented here permit culture of normal
human OSE under conditions in which the cells exhibit morphologic plasticity, variable 17β-HSD activity, and presence of keratin.
Supported by a grant and a research associateship to N. A. by the National Cancer Institute of Canada. 相似文献
5.
Pei XF Noble MS Davoli MA Rosfjord E Tilli MT Furth PA Russell R Johnson MD Dickson RB 《In vitro cellular & developmental biology. Animal》2004,40(1-2):14-21
We have established an explant-cell culture system for mammary gland tumors from c-myc oncogene-expressing transgenic mice and potentially other transgenic strains. By coating culture dish surfaces with fetal bovine serum and using culture media supplemented with low serum and growth factors, the mammary tumor specimens could be maintained in culture for over 3 mo. Throughout the culture period, the explants produced abundant outgrowths of epithelial cells. As the outgrowths of epithelial cells filled the dishes, the explants were serially transferred from one dish to another-a process that could be repeated at least six times, thus providing a continuous supply of primary tumor cells. This culture system provides a useful tool for studying the biology of mouse mammary gland tumors and possibly tumors from other organ sites. 相似文献
6.
J. M. Zahm E. Lamiot D. Pierrot M. Chevillard J. Hinnrasky E. Puchelle 《In vitro cellular & developmental biology. Plant》1990,26(11):1063-1067
Summary Ciliated cell cultures can be produced in outgrowths from explants of human respiratory epithelium. An image analysis technique
was develope to quantify the percentage of active ciliated cells present in these cultures. The subtraction 2 by 2 of five
successive video images of the cultures, followed by the addition of the resulting images, allowed the determinaton of the
culture surface covered by ciliated cells. The percentage of this surface varied according to the regions of the explant (27.7%
in the outgrowth near the explant and 4.1% at the periphery of the outgrowth). High variations were observed within the same
region of an outgrowth, as well as from one outgrowth to another. However, maximal differentiation was observed after 4 d
of culture. The quantitation techniques described in the present work might be useful for studying in vitro the respiratory
epithelial injury and the subsequent repair processes.
This work was supported by CEB-INSERM and SYNTHELABO-INSERM grants. 相似文献
7.
Rebecca J. Morris Amy C. Haynes Susan M. Fischer Thomas J. Slaga 《In vitro cellular & developmental biology. Animal》1991,27(11):886-895
Summary A chemically defined medium containing 1.2 mM Ca2+ has been developed for the culture of primary epidermal keratinocytes from untreated adult mice such that proliferation is
accompanied by the formation of desmosomes and stratification. Cultured cutaneous explants of 1 mm2 from the backs of untreated, control, and carcinogen-exposed mice all demonstrated epithelial outgrowth within 1 wk, and
by 5 wk approached confluence with characteristics of terminal differentiation such as desmosomes and stratification. Addition
of 12-O-tetradecanoylphorbol-13-acetate (TPA) to the medium in concentrations of 0.001, 0.01, and 0.1 μg/ml resulted in a delay of
approximately 1 wk in the outgrowth of the explants compared with the acetone controls and in a 30% decrease in the diameter
of the epithelial outgrowth at 3 wk. The inhibition in outgrowth was overcome at higher concentrations (0.5, 1.0, and 10 μg/ml
TPA). No obvious differences in morphology or in the rate of epidermal outgrowth within a 5-wk interval among explants from
normal untreated epidermis, epidermis from mice treated with acetone, or epidermis from mice treated with an initiating application
of 7,12-dimethylbenz[a]anthracene were observed. The defined composition of this medium and its ability to support reproducibly and conveniently
both proliferation and differentiation of normal as well as treated primary adult murine epidermal cells suggest that it should
be useful for a number of studies not previously possible that are relevent to the biology of the skin, to toxicology, and
to carcinogenesis in the murine model system. 相似文献
8.
J M Zahm D Pierrot J Hinnrasky C Fuchey M Chevillard D Gaillard E Puchelle 《Biorheology》1990,27(3-4):559-565
Primary cultures of respiratory epithelium were produced as outgrowths from human fetal and adult tracheal and nasal polyp explants. Video recordings of the epithelial cell outgrowths were carried out after 5 days of culture and the ciliary beating frequency was analyzed by using a video technique. Uniform fields of differentiated ciliated cells were observed near the edge of the explant. In the transition region of the outgrowth from the explant to the outgrowth periphery, isolated ciliated cells were present, as well as cells with fused cilia. The ciliary beating frequency of the outgrowth of well-differentiated ciliated cells (13.5 +/- 1.4 Hz) was significantly higher (p less than 0.001) than the beating frequency of both the explant (11.9 +/- 0.7 Hz) and the ciliated cells with fused cilia (9.8 +/- 1.7 Hz). The same differentiation stages and functional activities were observed in the outgrowth cultures, whatever their origin. These in vitro models are comparable with each other and therefore could be useful for studying the ciliogenesis and functional activity of the human respiratory epithelium. 相似文献
9.
Pretransplantation cultivation of adult human skin has been optimized for rapid and prolonged outgrowth of epidermal cells from tissue explants using autonomic-perfusion, thin-layer culture technology (steady-state). This system fostered growth of autologous mesenchymal elements via critical control of the culture environment. The resulting cellular outgrowth maintained a balanced epithelial-dermal relationship, contained keratinocytes as well as minority epidermal cells, melanocytes and possibly Langerhans cells. Critical control of culture pH and osmolarity was found to enhance epithelial cell proliferation. 相似文献
10.
Summary Radiation is known to be carcinogenic to humans but attempts to demonstrate the process using human tissue culture models have met with little success. In the present study explants were established from urothelium and exposed to radiation and a range of chemical carcinogens, suspected promotor or metabolic agents. The resulting outgrowth was monitored for growth rate, proliferating epithelial fraction and development and differentiation of endothelial cells in culture. The results indicate that enhanced growth of epithelial cells can be seen when cultures are irradiated in the presence of various nitrosamines, benzo(a)pyrene or aniline. Radiation alone reduced the overall growth area measured but several proliferative foci developed on the resulting outgrowth. Their ultrastructural appearance reveals that they carry severe mitochondrial damage and exposure of treated cultures to metabolic inhibitors confirms that their respiration is defective. Endothelial cells proliferated over the surface of the epithelial monolayer and both the number and the degree of differentiation of the endothelial cells increased with increasing dose up to 10 Gy. While the cultures are not immortalised by the treatment, it appears that the epithelial cells have an extended lifespan (division capacity) and that a subpopulation has undergone a number of premalignant changes. Changes in endothelial cell proliferation also occur. 相似文献
11.
Nikolay K. Popnikolov Jason Yang Raphael C. Guzman Steven M. Swanson Gudmundur Thordarson Gail Collins Frank Talamantes Satyabrata Nandi 《Journal of cellular physiology》1995,163(1):51-60
A new system for studying growth of normal human mammary epithelial cells in an in vivo environment using athymic nude mice is described. Human mammary epithelial cells dissociated from reduction mammoplasty specimens were embedded within collagen gels and subsequently transplanted subcutaneously into nude mice. Histological sections of recovered collagen gels showed epithelial cells arranged as short tubules with some branching. Proliferation of mammary epithelial cells was quantitated in vivo by 3 days' continuous infusion with 5 bromo-2′-deoxy-uridine followed by immunostaining of sections from recovered gels. Ovarian steroids administered to the host animals, resulting in blood serum levels normally found in the human female, had little or no effect on the proliferation of human mammary epithelial cells. Collagen gel embedded mouse mammary epithelial cells, mouse mammary explants, and host mammary glands all responded similarly to ovarian steroids, suggesting that the unresponsiveness of the human mammary epithelial cells under these conditions was not due to dissociation per se. However, an increased dose of 17β-estradiol or a growth factor combination containing epidermal growth factor, cholera toxin, and cortisol significantly stimulated the proliferation of human outgrowths. The growth factor response was dependent on the location of the cells, with the greatest response seen in the part of the gel proximal to the osmotic pump delivering the growth factors and the effect gradually waning in area more distal to the pump. The effect was especially striking since the mitotic figures could be easily identified and the labeling index was as high as 75%. The host mouse mammary gland also responded to growth factors, resulting in ductal hyperplasia. The proliferative and morphogenetic effects of various agents on normal human mammary epithelial cells embedded in collagen gel can be studied in vivo in nude mice. © 1995 Wiley-Liss, Inc. 相似文献
12.
James H. Resau Patricia C. Phelps Si-min. Zhu Duane Smoot Hsiang-Kuang Lee John R. Cottrell Eric A. Hudson Kathryn A. Elliget 《Cytotechnology》1990,3(1):61-73
Human esophageal epithelium obtained from intermediate autopsies (<12 h) was maintained as cell and explant cultures. In order to develop a serum-free, defined media culture model, several medias and additives were evaluated. The viability and differentiation of the epithelial cells cultured with serum-free, Keratinocyte Growth Media (KGM, Clonetics Co., San Diego, CA) was improved over that of esophageal cells and explants cultured in either serum-supplemented CMRL 1066 (OCM), serum-free additive-supplemented CMRL 1066, or cimetidine-supplemented CMRL 1066. The KGM component EGF was determined to be trophic for esophagus cells on the basis of findings of increased 3H-TdR tabelling in KGM cultures when compared to control cells grown in KGM without EGF (KBM). The morphologic pattern of the cytoskeletal proteins actin, keratin, and vimentin were characterized in isolated cell populations. The intermediate filaments, keratin, and vimentin were co-expressed in these epithelial cells. Esophageal explant viability, differentiation, and outgrowth from 15 cases were also evaluated in dishes coated with basement membrane associated proteins. Explants cultured in these dishes were equally well-preserved and differentiated. There were no significant differences in the explant histology when there was protein coating of the culture dishes, although one case showed improved outgrowth with laminin coating. A main advantage for using this culture system is that the same medium (KGM) can be used for both the culture of explants and isolated epithelial cells. Future applications of this model include determining: (1) the effect different concentrations of EGF and calcium in the media will have on esophageal proliferation and differentiation, and (2) the role of different basement membrane associated proteins on the plating efficiency of either isolated or outgrowth epithelial esophageal cells.This is publication #2544 from the Pathobiology Laboratory. 相似文献
13.
Mammary tumours in female BR6/Icrf mice and the corresponding contralateral normal mammary glands were disaggregated with collagenase and the epithelial structures released ('organoids') separated from other cellular components by filtration. The organoids were established in primary culture in a collagen matrix and the outgrowths obtained were studied by light microscopy and time-lapse cinemicroscopy. The pattern of three-dimensional outgrowths produced was found to be specific to the original tissue. Organoids from normal tissue formed a characteristic outgrowth designated Pattern A. Normal tissue from pregnant mice formed an additional characteristic outgrowth (Pattern A') which has not been described previously. Pregnancy-dependent tumours produced a distinctive phenotypic outgrowth designated Pattern D, whereas pregnancy-independent tumours gave a different distinctive Pattern B as well as a unique specific outgrowth designated Pattern C. Outgrowths of Pattern D from a pregnancy-dependent tumour were removed from culture and implanted into a syngeneic female mouse. Tumours arising in the host were found to be pregnancy-independent and showed phenotypic outgrowths in subsequent culture of pregnancy-independent Patterns B and C. The results show that the type of outgrowths in these cultures correlates with the biology of the tissue in vivo and that changes in tumour progression in vivo are accompanied by alterations in phenotypic outgrowths in culture. 相似文献
14.
Terminally differentiating epithelial tissues in primary explant culture: A model of growth and development 总被引:2,自引:0,他引:2
Donald J. Merchant 《In vitro cellular & developmental biology. Plant》1990,26(6):543-553
Summary Many epithelial tissues are characterized by the presence of basal cells which serve the dual roles of self-renewal and of
progression through terminal differentiation, to a functional state. Such tissues, when grownin vitro as primary explants, exhibit a characteristic pattern of outgrowth and development which includes both renewal and efforts
toward normal differentiation. The degree of differentiation achieved depends upon conditions of culture and may be modulated
in a variety of ways. The human prostate constitutes such a system and offers numerous possibilities for investigating basic
control mechanisms in growth and development. Information on a variety of epithelial tissues is reviewed and experimental
results using human prostate tissue are presented.
The work was supported by grant R26 CA 2365, National Cancer Institute. 相似文献
15.
Yukio Kondo Ian Rose Graeme P. Young Robert H. Whitehead 《Experimental cell research》1984,153(1):121-134
Explants of small intestinal tissue have been cultured from fetal and young rats (from 13-day fetuses to 3-week-old rats). Growth of morphologically typical epithelial cells was obtained from explants of tissue from 14–20 day fetuses. Optimal growth was obtained using tissue from 17-day fetuses with outgrowth from the explant being observed 1-day after explant. Eighty per cent of explants developed epithelial growth by 11 days in culture. Initially, the epithelial outgrowth showed no morphological evidence of differentiation but after 5–10 days in culture differentiation into goblet or elongated cells with alkaline phosphatase activity occurred. Cells with brush borders and goblet cells were identified using electron microscopy. No differentiation occurred if the explant was removed even though growth continued.It was very difficult to culture tissue from fetuses older than 20 days' gestation, and when small intestine of 18–20-day fetuses was divided into two parts (proximal and distal) and cultured separately, growth of epithelial cells from explants of the proximal segment was less successful than that of the distal segment, indicating that the growth ability of these epithelial cells in vitro was closely related to tissue maturation in vivo. In contrast to the apparent relationship between fetal age and successful growth of intestinal epithelial cells, squamous epithelial cells of the esophagus could be grown from explants of 14-day fetus through newborn and 3-week-old rats. 相似文献
16.
Most human ovarian cancers are thought to arise in the ovarian surface epithelium (OSE). The precise role of OSE in carcinogenesis has not been defined because no appropriate animal models for the study of this tissue exist and culture of human OSE has been limited to primary outgrowths. In this report, we describe conditions for serial cultivation of normal human OSE. Premenopausal ovarian tissue was obtained at surgery. OSE growth was compared in media MCDB 202, 199 and Waymouth's 752/1 (WM) supplemented with 5, 15, or 25% fetal bovine serum (FBS), with/without 20 ng/ml epidermal growth factor (EGF) and 0.4 micrograms/ml hydrocortisone (HC). The rate and extent of OSE outgrowths from explants in primary culture were greatest in either WM or 199/202 (1:1), supplemented with 15% FBS/EGF/HC. In early passage cultures, cell proliferation was most rapid and extensive in 199/202 with 15% FBS, EGF, and HC. In this medium, OSE cells were subcultured up to 10 times and underwent 20-25 population doublings over 5 weeks. The population doubling time during rapid growth was approximately 48 h. Seeding efficiencies of up to 53% and cloning efficiencies of up to 13% were obtained. Early passage OSE cells reversibly modulated from a slow growing, epithelial, intensely keratin-positive form in 199/202 medium lacking EGF/HC, to a rapidly proliferating, elongate, less keratin-positive form in medium with EGF/HC. OSE cells grown in WM/5-15% FBS were epithelial and near-stationary. Thus, culture conditions have been defined for ovarian carcinogen assays requiring either proliferating or stationary cell populations, and for further studies of the role of OSE in ovarian biology. 相似文献
17.
Enhancement of Neurite Outgrowth and Aspartate-Glutamate Uptake Systems in Retinal Explants Cultured with Chick Gizzard Extract 总被引:3,自引:2,他引:1
Satoru Kato Koroku Negishi Yokichi Hayashi Naomasa Miki 《Journal of neurochemistry》1983,40(4):929-938
Chicken gizzard extract promoted a long and radially directed neurite outgrowth from retinal explants of 8-day-old chick embryo in cultures of 2–3 days. The neurite outgrowth from retinal explants cultured in the absence of gizzard extract was short and restricted to the explant perimeter. The neurite outgrowth promoted by gizzard extract depended strictly on several factors. (a) Fetal calf serum and polycationic substratum were required in this culture system, (b) Pretreatment of the polyornithine-coated substratum with gizzard extract allowed the retinal explants to extend neurites even in the absence of gizzard extract in the medium. (c) Maximal neurite outgrowth was observed in retinal explants dissected from 8-day embryos, but thereafter the explants’response to gizzard extract rapidly declined and was almost lost at the 12th day. As a biochemical parameter of differentiation of cultured neuroretina, uptake systems for neurotransmitter candidates were examined in homogenates of retinal explants cultured in the absence or presence of gizzard extract. After 3 days in culture with gizzard extract, the uptake increased for aspartate and glutamate 1.6- to 1.8-fold and for γ-aminobutyric acid to a lesser degree when examined at a concentration for high-affinity uptake (10-6M). In contrast, the uptake capacity for glycine, choline, and dopamine was not altered in explants cultured with or without gizzard extract. Kinetic analysis showed that the enhanced capacity to accumulate aspartate was not due to an alteration of Km, but to an increase of Vmax. The results suggest that one or several factors in chick gizzard muscle promote not only neurite outgrowth but also the aspartate-glutamate uptake systems in the developing neuroretina, probably related to ganglion cells. 相似文献
18.
Mark A. LaBarge James C. Garbe Martha R. Stampfer 《Journal of visualized experiments : JoVE》2013,(71)
Experimental examination of normal human mammary epithelial cell (HMEC) behavior, and how normal cells acquire abnormal properties, can be facilitated by in vitro culture systems that more accurately model in vivo biology. The use of human derived material for studying cellular differentiation, aging, senescence, and immortalization is particularly advantageous given the many significant molecular differences in these properties between human and commonly utilized rodent cells1-2. Mammary cells present a convenient model system because large quantities of normal and abnormal tissues are available due to the frequency of reduction mammoplasty and mastectomy surgeries.The mammary gland consists of a complex admixture of many distinct cell types, e.g., epithelial, adipose, mesenchymal, endothelial. The epithelial cells are responsible for the differentiated mammary function of lactation, and are also the origin of the vast majority of human breast cancers. We have developed methods to process mammary gland surgical discard tissues into pure epithelial components as well as mesenchymal cells3. The processed material can be stored frozen indefinitely, or initiated into primary culture. Surgical discard material is transported to the laboratory and manually dissected to enrich for epithelial containing tissue. Subsequent digestion of the dissected tissue using collagenase and hyaluronidase strips stromal material from the epithelia at the basement membrane. The resulting small pieces of the epithelial tree (organoids) can be separated from the digested stroma by sequential filtration on membranes of fixed pore size. Depending upon pore size, fractions can be obtained consisting of larger ductal/alveolar pieces, smaller alveolar clusters, or stromal cells. We have observed superior growth when cultures are initiated as organoids rather than as dissociated single cells. Placement of organoids in culture using low-stress inducing media supports long-term growth of normal HMEC with markers of multiple lineage types (myoepithelial, luminal, progenitor)4-5. Sufficient numbers of cells can be obtained from one individual''s tissue to allow extensive experimental examination using standardized cell batches, as well as interrogation using high throughput modalities.Cultured HMEC have been employed in a wide variety of studies examining the normal processes governing growth, differentiation, aging, and senescence, and how these normal processes are altered during immortal and malignant transformation4-15,16. The effects of growth in the presence of extracellular matrix material, other cell types, and/or 3D culture can be compared with growth on plastic5,15. Cultured HMEC, starting with normal cells, provide an experimentally tractable system to examine factors that may propel or prevent human aging and carcinogenesis. 相似文献
19.
Sung-Goo Chang Károly Tóth Jennifer D. Black Harry K. Slocum Scott D. Perrapato Robert P. Huben Youcef M. Rustum 《In vitro cellular & developmental biology. Animal》1992,28(2):128-135
Summary A model system for 3-dimensional “native-state” culture of tissues on collagen gels (Proc. Natl. Acad. Sci. USA 86:2013–2017;
1989) has been applied in this study to histologically normal human renal cortical tissue from 11 patients undergoing nephrectomy
for renal cell carcinoma elsewhere in the kidney. Microbial contamination occurred in 12/90 cultures, the rest (78) were studied
by visual inspection, histology, immunohistochemical analysis for pankeratin (epithelial cell origin), vimentin (mesenchymal
cell origin), andp-glycoprotein (associated with proximal tubules), transmission electron microscopy (EM), incorporation of tritiated thymidine
(3HTdR). In the first 10 days, explants showed3HTdR-labeled cells in tubule structures. The surrounding gel was invaded by cells forming tubule structures, sometimes with
basement membrane. Some of these cells showed labeling by3HTdR and immunostaining positive for pankeratin andp-glycoprotein. EM showed well-polarized epithelial cells in tubule structures with tight junctions, interdigitating lateral
processes, and microvilli characteristic of proximal and distal convoluted tubules.3HTdR-labeled cells in tubule structures were observed even 2 mo. after Passage 1, 6 mo. after the initial explantation. Tubule
growth was most active and fibroblast proliferation was negligible from 2 to 4 wk postexplantation. The proliferation of tubulelike
cells and formation of tubulelike structures in this system represents an opportunity to study human renal cortical tissue
in vitro, under conditions more closely resembling in vivo circumstances than are present in other in vitro systems suitable
for long-term study. This model has potential use for in vitro toxicology studies and studies of renal physiology. 相似文献
20.
Kristina Sundqvist Yun Liu Kristina Arvidson Kari Ormstad Lennart Nilsson Rune Toftgård Roland C. Grafström 《In vitro cellular & developmental biology. Animal》1991,27(7):562-568
Summary Human buccal epithelial cells have been reared from explants maintained in supplemented MCDB 153 medium. Primary epithelial
outgrowths show typical structural features and uniformly express keratins; subunit analyses demonstrate expression of keratins
5, 6, 14, 16/17, and 19. The cells exhibit up to 6% colony forming efficiency and divide at about 0.8 population doublings
per day on fibronectin/collagen-coated dishes at clonal density. Studies of markers of proliferation and differentiation in
buccal epithelial cells indicate that epidermal growth factor, cholera toxin, retinoic acid, and pituitary extract each exhibit
a distinctive ability to enhance growth and variably affect cell migration and cell surface area. Transforming growth factorβ-1 inhibits growth and increases surface area without affecting migration, involucrin expression, and cross-linked envelope
formation. Moreover, exposure of cells to fetal bovine serum, the tumor promoting agent 12-O-tetradecanoylphorbol-13-acetate or an elevated Ca2+ concentration (from 0.1 to 1 mM) inhibits growth and induces squamous differentiation as indicated by inhibition of migration, increases in surface area,
involucrin expression, or formation of cross-linked envelopes. The results show that epithelial cells can be reproducibly
derived from explant cultures of human buccal mucosa specimens and the cells transferred under serum-free conditions. Buccal
epithelial cells in culture undergo a pattern of growth and differentiation that mimics parakeratinization in vivo and variably
respond to several agents shown to modulate growth of cells that originate from other types of epithelia.
This work was supported in part by grants from the Swedish National Board of Laboratory Animals, the Swedish Medical Research
Council, the Swedish Natural Science Research Council, the Swedish Fund for Scientific Research Without Animal Experiments,
the Swedish Cancer Society, the Swedish Tobacco Company, and Lions Club International, Djurg?rden, Stockholm, Sweden. 相似文献