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1.
Jennifer Häberle Josef Holzapfel Günther Schweizer Lorenz Hartl 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(2):325-332
We report on the verification of a resistance quantitative trait locus (QTL) on chromosome 1BL (now designated Qfhs.lfl-1BL) which had been previously identified in the winter wheat cultivar Cansas. For a more precise estimation of the QTL effect
and its influence on plant height and heading date lines with a more homogeneous genetic background were created and evaluated
in four environments after spray inoculation with Fusarium culmorum. Qfhs.lfl-1BL reduced FHB severity by 42% relative to lines without the resistance allele. This QTL did not influence plant height, but
significantly delayed heading date by one day. All of the most resistant genotypes of the verification population carried
this major QTL displaying its importance for disease resistance. This resistance QTL has not only been found in the cultivar
Cansas, but also in the three European winter wheat cultivars Biscay, History and Pirat. A subsequent meta-analysis confirmed
the presence of a single QTL on the long arm of chromosome 1B originating from the four mentioned cultivars. Altogether, the
results of the present study indicate that Qfhs.lfl-1BL is an important component of FHB resistance in European winter wheat and support the view that this QTL would be effective
and valuable in backcross breeding programmes.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
2.
Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat 总被引:2,自引:0,他引:2
Xunfen Chen Justin D. Faris Jinguo Hu Robert W. Stack Tika Adhikari Elias M. Elias Shahryar F. Kianian Xiwen Cai 《Molecular breeding : new strategies in plant improvement》2007,19(2):113-124
Fusarium head blight (FHB) is a devastating disease of cultivated wheat worldwide. Partial resistance to FHB has been identified
in common wheat (Triticum aestivum L.). However, sources of effective FHB resistance have not been found in durum wheat (T. turgidum L. var. durum). A major FHB resistance quantitative trait loci (QTL), Qfhs.ndsu-3AS, was identified on chromosome 3A of T.
dicoccoides, a wild relative of durum wheat. Here, we saturated the genomic region containing the QTL using EST-derived target region
amplified polymorphism (TRAP), sequence tagged site (STS), and simple sequence repeat (SSR) markers. A total of 45 new molecular
marker loci were detected on chromosome 3A and the resulting linkage map consisted of 55 markers spanning a genetic distance
of 277.2 cM. Qfhs.ndsu-3AS was positioned within a chromosomal interval of 11.5 cM and is flanked by the TRAP marker loci, Xfcp401 and Xfcp397.2. The average map distance between the marker loci within this QTL region was reduced from 4.9 cM in the previous study to
3.5 cM in the present study. Comparative mapping indicated that Qfhs.ndsu-3AS is not homoeologous to Qfhs.ndsu-3BS, a major FHB QTL derived from the common wheat cultivar Sumai 3. These results facilitate our efforts toward map-based cloning
of Qfhs.ndsu-3AS and utilization of this QTL in durum wheat breeding via marker-assisted selection. 相似文献
3.
The devastating effect of Fusarium head blight (FHB) caused by Fusarium graminearum has led to significant financial losses across the Upper Midwest of the USA. These losses have spurred the need for research in biological, chemical, and genetic control methods for this disease. To date, most of the research on FHB resistance has concentrated on hexaploid wheat (Triticum aestivum L.) lines originating from China. Other sources of resistance to FHB would be desirable. One other source of resistance for both hexaploid wheat and tetraploid durum wheat (T. turgidum L. var. durum) is the wild tetraploid, T. turgidum L. var. dicoccoides (T. dicoccoides). Previous analysis of the `Langdon'-T. dicoccoides chromosome substitution lines, LDN(Dic), indicated that the chromosome 3A substitution line expresses moderate levels of resistance to FHB. LDN(Dic-3A) recombinant inbred chromosome lines (RICL) were used to generate a linkage map of chromosome 3A with 19 molecular markers spanning a distance of 155.2 cM. The individual RICL and controls were screened for their FHB phenotype in two greenhouse seasons. Analysis of 83 RICL identified a single major quantitative trait locus, Qfhs.ndsu-3AS, that explains 37% of the phenotypic or 55% of the genetic variation for FHB resistance. A microsatellite locus, Xgwm2, is tightly linked to the highest point of the QTL peak. A region of the LDN (Dic-3A) chromosome associated with the QTL for FHB resistance encompasses a 29.3 cM region from Xmwg14 to Xbcd828. 相似文献
4.
A major QTL for resistance to Fusarium head blight (FHB) in wheat, Qfhs.ndsu-3BS, has been identified and verified by several research groups. The objective of this study was to increase the marker density in this QTL region using STS (sequence-tagged site) markers developed from wheat expressed sequence tags (ESTs) near Qfhs.ndsu-3BS. Because wheat chromosome 3BS and rice chromosome 1S are syntenous, the sequences of P1-derived artificial chromosome (PAC) and (or) bacterial artificial chromosome (BAC) clones covering the sub-distal portion of rice chromosome 1S were used as queries for a BLASTn search to identify wheat ESTs most likely near Qfhs.ndsu-3BS. Sixty-eight out of 79 STS primer pairs designed from wheat ESTs amplified PCR products from the genomic DNA of Triticum aestivum 'Chinese Spring'. Twenty-eight STS markers were localized on chromosome 3BS by aneuploid analysis. Six out of the nine STS markers that could be mapped in the T. aestivum 'Sumai 3'/T. aestivum 'Stoa' population had higher R2 and LOD values for this QTL than the most significant marker reported previously. Therefore, leveraging genome sequence information available in rice for wheat genetics is an effective strategy to develop DNA markers for Qfhs.ndsu-3BS, and this strategy may have broad applications for targeted mapping of other traits in cereal crops. 相似文献
5.
Molecular mapping of QTL for Fusarium head blight resistance introgressed into durum wheat 总被引:1,自引:0,他引:1
Mingxia Zhao Yueqiang Leng Shiaoman Chao Steven S. Xu Shaobin Zhong 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(9):1939-1951
Key message
The major QTL for FHB resistance from hexaploid wheat line PI 277012 was successfully introgressed into durum wheat and minor FHB resistance QTL were detected in local durum wheat cultivars. A combination of these QTL will enhance FHB resistance of durum wheat.Abstract
Fusarium head blight (FHB), caused by Fusarium graminearum, is a devastating disease of durum wheat. To combat the disease, great efforts have been devoted to introgress FHB resistance from its related tetraploid and hexaploid wheat species into adapted durum cultivars. However, most of the quantitative trait loci (QTL) for FHB resistance existing in the introgression lines are not well characterized or validated. In this study, we aimed to identify and map FHB resistance QTL in a population consisting of 205 recombinant inbred lines from the cross between Joppa (a durum wheat cultivar) and 10Ae564 (a durum wheat introgression line with FHB resistance derived from the hexaploid wheat line PI 277012). One QTL (Qfhb.ndwp-2A) from Joppa and two QTL (Qfhb.ndwp-5A and Qfhb.ndwp-7A) from 10Ae564 were identified through phenotyping of the mapping population for FHB severity and DON content in greenhouse and field and genotyping with 90K wheat Infinium iSelect SNP arrays. Qfhb.ndwp-2A explained 14, 15, and 9% of the phenotypic variation, respectively, for FHB severity in two greenhouse experiments and for mean DON content across the two greenhouse environments. Qfhb.ndwp-5A explained 19, 10, and 7% of phenotypic variation, respectively, for FHB severity in one greenhouse experiment, mean FHB severity across two field experiments, and mean DON content across the two greenhouse experiments. Qfhb.ndwp-7A was only detected for FHB severity in the two greenhouse experiments, explaining 9 and 11% of the phenotypic variation, respectively. This study confirms the existence of minor QTL in North Dakota durum cultivars and the successful transfer of the major QTL from PI 277012 into durum wheat.6.
QTL mapping of Fusarium head blight resistance in three related durum wheat populations 总被引:2,自引:0,他引:2
Noemie Prat Camille Guilbert Ursa Prah Elisabeth Wachter Barbara Steiner Thierry Langin Olivier Robert Hermann Buerstmayr 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(1):13-27
Key message
The QTL Fhb1 was successfully introgressed and validated in three durum wheat populations. The novel germplasm and the QTL detected will support improvement of Fusarium resistance in durum wheat.Abstract
Durum wheat (Triticum durum Desf.) is particularly susceptible to Fusarium head blight (FHB) and breeding for resistance is hampered by limited genetic variation within this species. To date, resistant sources are mainly available in a few wild relative tetraploid wheat accessions. In this study, the effect of the well-known hexaploid wheat (Triticum aestivum L.) quantitative trait locus (QTL) Fhb1 was assessed for the first time in durum wheat. Three F7-RIL mapping populations of about 100 lines were developed from crosses between the durum wheat experimental line DBC-480, which carries an Fhb1 introgression from Sumai-3, and the European T. durum cultivars Karur, Durobonus and SZD1029K. The RILs were evaluated in field experiments for FHB resistance in three seasons using spray inoculation and genotyped with SSR as well as genotyping-by-sequencing markers. QTL associated with FHB resistance were identified on chromosome arms 2BL, 3BS, 4AL, 4BS, 5AL and 6AS at which the resistant parent DBC-480 contributed the positive alleles. The QTL on 3BS was detected in all three populations centered at the Fhb1 interval. The Rht-B1 locus governing plant height was found to have a strong effect in modulating FHB severity in all populations. The negative effect of the semi-dwarf allele Rht-B1b on FHB resistance was compensated by combining with Fhb1 and additional resistance QTL. The successful deployment of Fhb1 in T. durum was further substantiated by assessing type 2 resistance in one population. The efficient introgression of Fhb1 represents a significant step forward for enhancing FHB resistance in durum wheat.7.
Detection of Fusarium head blight resistance QTL in a wheat population using bulked segregant analysis 总被引:10,自引:0,他引:10
Shen X Zhou M Lu W Ohm H 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,106(6):1041-1047
A population of 218 recombinant inbred lines (RILs) was developed from the cross of two wheat (Triticum aestivum L.) cultivars, 'Ning 894037' and 'Alondra'. Ning 894037 has resistance to Fusarium head blight (FHB) and Alondra is moderately susceptible. Response of the RILs and their parental lines to FHB infection was evaluated with point inoculation in four experiments both in greenhouse and in field conditions. Distribution of disease severity in the population is continuous, indicating quantitative inheritance of resistance to FHB. Bulked segregant analysis and QTL mapping based on simple sequence repeat (SSR) markers revealed three chromosome regions that are responsible for FHB resistance. A chromosome region on 3BS accounted for 42.5% of the phenotypic variation for FHB resistance. Additional QTLs were located on chromosomes 2D and 6B. These three QTLs jointly accounted for 51.6% of the phenotypic variation. SSR markers linked to the QTLs influencing resistance to FHB have potential for use in breeding programs. 相似文献
8.
QTL associated with Fusarium head blight resistance in the soft red winter wheat Ernie 总被引:2,自引:0,他引:2
Liu S Abate ZA Lu H Musket T Davis GL McKendry AL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(3):417-427
Fusarium head blight (FHB), mainly caused by Fusarium graminearum Schwabe [telomorph: Gibberella zeae Schw. (Petch)], is an increasingly important disease of wheat (Triticum aestivum L.). Host-plant resistance provides the best hope for reducing economic losses associated with FHB, but new sources of resistance
are limited. The moderately resistant winter wheat cultivar, Ernie, may provide a source of resistance that differs from Sumai
3 but these genes have not been mapped. Also hindering resistance breeding may be associations of resistance with agronomic
traits such as late maturity that may be undesirable in some production environments. This research was conducted to identify
QTL associated with type II FHB resistance (FHB severity, FHBS), and to determine if they are associated with days to anthesis
(DTA), number of spikelets (NOS), and the presence/absence of awns. Two hundred and forty-three F8 recombinant inbred lines from a cross between the resistant cultivar, Ernie and susceptible parent, MO 94-317 were phenotyped
for type II FHB resistance using point inoculation in the greenhouse during 2002 and 2003. Genetic linkage maps were constructed
using 94 simple sequence repeat (SSR) and 146 amplified fragment length polymorphic (AFLP) markers. Over years four QTL regions
on chromosomes 2B, 3B, 4BL and 5A were consistently associated with FHB resistance. These QTL explained 43.3% of the phenotypic
variation in FHBS. Major QTL conditioning DTA and NOS were identified on chromosome 2D. Neither the QTL associated with DTA
and NOS nor the presence/absence of awns were associated with FHB resistance in Ernie. Our results suggest that the FHB resistance
in Ernie appears to differ from that in Sumai 3, thus pyramiding the QTL in Ernie with those from Sumai 3 could result in
enhanced levels of FHB resistance in wheat. 相似文献
9.
A major quantitative trait locus (QTL) associated with resistance to Fusarium head blight (FHB) was identified on chromosome 3BS between simple sequence repeat (SSR) markers Xgwm389 and Xgwm493 in wheat “Ning 7840”, a derivative from “Sumai 3”. However, the marker density of SSR in the QTL region was much lower than that required for marker-assisted selection (MAS) and map-based cloning. The objective of this study was to exploit new markers to increase marker density in this QTL region by using single-strand conformational polymorphism (SSCP) markers developed from wheat-expressed sequence tags (ESTs) on 3BS bin 8-0.78-1.0. Sixty-nine out of 85 SSCP primer pairs amplified PCR (polymerase chain reaction) products from the genomic DNA of “Chinese Spring”. Thirty-four primer pairs amplified PCR products that could form clear ssDNA (single strand DNA) bands through denaturation treatment. Ten SSCP markers had polymorphisms between Ning 7840 and “Clark”. Five of the ten polymorphic SSCP markers were located on chromosome 3B by nullitetrasomic analysis. Three SSCP markers (Xsscp6, Xsscp20, and Xsscp21) were mapped into the region between Xgwm493 and Xgwm533 and possessed a higher coefficient of determination (R2) than Xgwm493 and Xgwm533. The SSCP markers, Xsscp6, Xsscp20, and Xsscp21, can be used for map-based cloning of the QTL and for marker-assisted selection in FHB resistance breeding. 相似文献
10.
A major quantitative trait locus (QTL) associated with resistance to Fusarium head blight (FHB) was identified on chromosome 3BS between simple sequence repeat (SSR) markers Xgwm389 and Xgwm493 in wheat 'Ning 7840', a derivative from 'Sumai 3'. However, the marker density of SSR in the QTL region was much lower than that required for marker-assisted selection (MAS) and map-based cloning. The objective of this study was to exploit new markers to increase marker density in this QTL region by using single-strand conformational polymorphism (SSCP) markers developed from wheat expressed sequence tags (ESTs) on 3BS bin 8-0.78-1.0. Sixty-nine out of 85 SSCP primer pairs amplified PCR (polymerase chain reaction) products from the genomic DNA of 'Chinese Spring'. Thirty-four primer pairs amplified PCR products that could form clear ssDNA (single strand DNA) bands through denaturation treatment. Ten SSCP markers had polymorphisms between 'Ning 7840' and 'Clark'. Five of the ten polymorphic SSCP markers were located on chromosome 3B by nulli-tetrasomic analysis. Three SSCP markers (Xsscp6, Xsscp20, and Xsscp21) were mapped into the region between Xgwm493 and Xgwm533, and possessed higher coefficient of determination (R2) than Xgwm493 and Xgwm533. The SSCP markers, Xsscp6, Xsscp20, and Xsscp21, can be used for map-based cloning of the QTL and for marker-assisted selection in FHB resistance breeding. 相似文献
11.
Novel quantitative trait loci (QTL) for Fusarium head blight resistance in wheat cultivar Chokwang 总被引:6,自引:0,他引:6
Yang J Bai G Shaner GE 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(8):1571-1579
Fusarium head blight (FHB) is one of the most destructive diseases in wheat. This study was to identify new quantitative trait
loci (QTL) for FHB resistance and the molecular markers closely linked to the QTL in wheat cultivar Chokwang. The primers
of 612 simple sequence repeats (SSRs) and 12 target-region-amplified polymorphism (TRAP) marker were analyzed between resistant
(Chokwang) and susceptible (Clark) parents. One hundred and seventy-two polymorphic markers were used to screen a population
of 79 recombinant inbred lines (RILs) derived from the cross of Chokwang and Clark. One major QTL, Qfhb.ksu-5DL1, was identified on chromosome 5DL. The SSR marker Xbarc 239 was mapped in the QTL region, and also physically located to the bin of 5DL1-0.60-0.74 by using Chinese Spring deletion
lines. Another QTL Qfhb.ksu-4BL1was linked to SSR Xbarc 1096 and tentatively mapped on 4BL. A QTL on 3BS, Qfhb.ksu-3BS1, was also detected with marginal significance in this population. Different marker alleles for these QTL were detected between
Chokwang and Sumai 3 and its derivatives. These results suggested that Chokwang contains new QTL for FHB resistance that are
different from those in Sumai 3. Pyramiding resistance QTL from various sources may enhance FHB resistance in wheat cultivars. 相似文献
12.
Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled-haploid spring wheat population. 总被引:12,自引:0,他引:12
Fusarium head blight (FHB) is one of the most important fungal wheat diseases worldwide. Understanding the genetics of FHB resistance is the key to facilitating the introgression of different FHB resistance genes into adapted wheat. The objectives of the present study were to detect and map quantitative trait loci (QTL) associated with FHB resistance genes and characterize the genetic components of the QTL in a doubled-haploid (DH) spring wheat population using both single-locus and two-locus analysis. A mapping population, consisting of 174 DH lines from the cross between DH181 (resistant) and AC Foremost (susceptible), was evaluated for type I resistance to initial infection during a 2-year period in spray-inoculated field trials, for Type II resistance to fungal spread within the spike in 3 greenhouse experiments using single-floret inoculation, and for resistance to kernel infection in a 2001 field trial. One-locus QTL analysis revealed 7 QTL for type I resistance on chromosome arms 2DS, 3AS, 3BS, 3BC (centromeric), 4DL, 5AS, and 6BS, 4 QTL for type II resistance on chromosomes 2DS, 3BS, 6BS, and 7BL, and 6 QTL for resistance to kernel infection on chromosomes 1DL, 2DS, 3BS, 3BC, 4DL, and 6BS. Two-locus QTL analysis detected 8 QTL with main effects and 4 additive by additive epistatic interactions for FHB resistance and identified novel FHB resistance genes for the first time on chromosomes 1DL, 4AL, and 4DL. Neither significant QTL by environment interactions nor epistatic QTL by environment interactions were found for either type I or type II resistance. The additive effects of QTL explained most of the phenotypic variance for FHB resistance. Marker-assisted selection for the favored alleles at multiple genomic regions appears to be a promising tool to accelerate the introgression and pyramiding of different FHB resistance genes into adapted wheat genetic backgrounds. 相似文献
13.
R. Prins J. Z. Groenewald G. F. Marais J. W. Snape R. M. D. Koebner 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(4):618-624
Amplified fragment length polymorphism (AFLP) markers were used to enrich the map of the wheat chromosomal region containing
the Thinopyrum-derived Lr19 leaf rust resistance gene. The region closest to Lr19 was targeted through the use of deletion and recombinant lines of the translocated segment. One of the AFLP bands thus identified
was converted into a sequence-tagged-site (STS) marker. This assay generated a 130-bp PCR fragment in all Lr19-carrying lines tested, except for one deletion mutant, while non-carrier template failed to amplify any product. This sequence
represents the first marker to map on the distal side of Lr19 on chromosome 7el1. The conversion process of AFLP fragments to STS markers was technically difficult, mainly because of the presence of contaminating
fragments. Various approaches were taken to reduce the frequency of false positives and to identify the correct clone. We
were able to formulate a general verification strategy prior to clone sequencing. Various other factors causing problems with
converting AFLP bands to an STS assays are also discussed.
Received: 15 September 2000 / Accepted: 5 January 2001 相似文献
14.
Liu S Zhang X Pumphrey MO Stack RW Gill BS Anderson JA 《Functional & integrative genomics》2006,6(2):83-89
A major quantitative trait locus (QTL), Qfhs.ndsu-3BS, for resistance to Fusarium head blight (FHB) in wheat has been identified and verified by several research groups. The objectives
of this study were to construct a fine genetic map of this QTL region and to examine microcolinearity in the QTL region among
wheat, rice, and barley. Two simple sequence repeat (SSR) markers (Xgwm533 and Xgwm493) flanking this QTL were used to screen for recombinants in a population of 3,156 plants derived from a single F7 plant heterozygous for the Qfhs.ndsu-3BS region. A total of 382 recombinants were identified, and they were genotyped with two more SSR markers and eight sequence-tagged
site (STS) markers. A fine genetic map of the Qfhs.ndsu-3BS region was constructed and spanned 6.3 cM. Based on replicated evaluations of homozygous recombinant lines for Type II FHB
resistance, Qfhs.ndsu-3BS, redesignated as Fhb1, was placed into a 1.2-cM marker interval flanked by STS3B-189 and STS3B-206. Primers of STS markers were designed from wheat
expressed sequence tags homologous to each of six barley genes expected to be located near this QTL region. A comparison of
the wheat fine genetic map and physical maps of rice and barley revealed inversions and insertions/deletions. This suggests
a complex microcolinearity among wheat, rice, and barley in this QTL region. 相似文献
15.
QTL analysis of Fusarium head blight resistance using a high-density linkage map in barley 总被引:2,自引:0,他引:2
Hori K Kobayashi T Sato K Takeda K 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(8):1661-1672
Fusarium head blight (FHB) resistance was evaluated in a set of recombinant inbred (RI) lines from a cross between Russia
6 (resistant) and H.E.S. 4 (susceptible), which had one of the widest differences of FHB resistance reactions among ca. 5,000
barley germplasm accessions in Okayama University. Field-grown spikes were sampled and inoculated by the ‘cut-spike test’.
Resistance reactions on the parents and RI lines were scored by eleven grades, from resistant (0) to susceptible (10). Quantitative
trait loci (QTL) analysis detected three QTL: two located on the long arm of chromosome 2H, and another on the short arm of
chromosome 5H. A QTL located on chromosome 2H was coincident with the vrs1 locus, which governs inflorescence row type. The other QTL on chromosome 2H was positioned in the vicinity of cleistogamy
locus (cly1 or Cly2) that determines inflorescence opening/closing. Resistant gene analog (RGA) and expressed sequence tag (EST) markers with
homology for disease resistance genes were integrated into the high-density linkage map. Most of these markers were not localized
near the identified resistance QTL, except for one RGA marker (FXLRRfor_XLRRrev170) localized in the vicinity of the cly1/Cly2 locus. Five AFLP markers localized in the vicinity of the identified QTL were sequenced to convert them into sequence tagged
site (STS) markers. Genotyping of each RI line using two AFLP–STS markers and the vrs1 locus indicated that the RI lines with three Russia 6 QTL alleles exhibited the same level of high FHB resistance reactions
as Russia 6. In contrast, RI lines with three susceptible alleles showed reactions close to H.E.S. 4. Therefore, the markers
closely linked to the QTL can be efficiently used for the selection of resistance. 相似文献
16.
Identification and molecular mapping of two QTLs with major effects for resistance to Fusarium head blight in wheat 总被引:1,自引:0,他引:1
Chu C Niu Z Zhong S Chao S Friesen TL Halley S Elias EM Dong Y Faris JD Xu SS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(7):1107-1119
Fusarium head blight (FHB) is a devastating disease of wheat worldwide. Novel sources of resistance are critical for improving
FHB resistance levels in wheat. From a large-scale evaluation of germplasm for reactions to FHB, we identified one wheat accession
(PI 277012) that consistently showed a high level of resistance in both greenhouse and field experiments. To characterize
the FHB resistance in this accession, we developed a doubled haploid (DH) mapping population consisting of 130 lines from
the cross between PI 277012 and the hard red spring wheat cultivar ‘Grandin’. The DH population was then evaluated for reactions
to FHB in three greenhouse seasons and five field environments. Based on a linkage map that consisted of 340 SSR markers spanning
2,703 cM of genetic distance, two major quantitative trait loci (QTLs) for FHB resistance were identified on chromosome arms
5AS and 5AL, with each explaining up to 20 and 32% of the variation in FHB severity, respectively. The two QTLs also showed
major effects on reducing the percentage of Fusarium damaged kernels (FDK) and deoxynivalenol (DON) accumulation in seeds.
FHB resistance has not previously been reported to be associated with this particular genomic region of chromosome arm 5AL,
thus indicating the novelty of FHB resistance in PI 277012. Plant maturity was not associated with FHB resistance and the
effects of plant height on FHB resistance were minor. Therefore, these results suggest that PI 277012 is an excellent source
for improving FHB resistance in wheat. The markers identified in this research are being used for marker-assisted introgression
of the QTLs into adapted durum and hard red spring wheat cultivars. 相似文献
17.
J. A. Anderson R. W. Stack S. Liu B. L. Waldron A. D. Fjeld C. Coyne B. Moreno-Sevilla J. Mitchell Fetch Q. J. Song P. B. Cregan R. C. Frohberg 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(8):1164-1168
Genetic resistance to Fusarium head blight (FHB), caused by Fusarium graminearum, is necessary to reduce the wheat grain yield and quality losses caused by this disease. Development of resistant cultivars
has been slowed by poorly adapted and incomplete resistance sources and confounding environmental effects that make screening
of germplasm difficult. DNA markers for FHB resistance QTLs have been identified and may be used to speed the introgression
of resistance genes into adapted germplasm. This study was conducted to identify and map additional DNA markers linked to
genes controlling FHB resistance in two spring wheat recombinant inbred populations, both segregating for genes from the widely
used resistance source ’Sumai 3’. The first population was from the cross of Sumai 3/Stoa in which we previously identified
five resistance QTLs. The second population was from the cross of ND2603 (Sumai 3/Wheaton) (resistant)/ Butte 86 (moderately
susceptible). Both populations were evaluated for reaction to inoculation with F. graminearum in two greenhouse experiments. A combination of 521 RFLP, AFLP, and SSR markers were mapped in the Sumai 3/Stoa population
and all DNA markers associated with resistance were screened on the ND2603/Butte 86 population. Two new QTL on chromosomes
3AL and 6AS wer found in the ND2603/Butte 86 population, and AFLP and SSR markers were identified that explained a greater
portion of the phenotypic variation compared to the previous RFLP markers. Both of the Sumai 3-derived QTL regions (on chromosomes
3BS, and 6BS) from the Sumai 3/Stoa population were associated with FHB resistance in the ND2603/Butte 86 population. Markers
in the 3BS QTL region (Qfhs.ndsu-3BS) alone explain 41.6 and 24.8% of the resistance to FHB in the Sumai 3/Stoa and ND2603/Butte 86 populations, respectively.
This region contains a major QTL for resistance to FHB and should be useful in marker-assisted selection.
Received: 17 August 2000 / Accepted: 16 October 2000 相似文献
18.
Association mapping for Fusarium head blight resistance in European soft winter wheat 总被引:1,自引:0,他引:1
Thomas Miedaner Tobias Würschum Hans P. Maurer Viktor Korzun Erhard Ebmeyer Jochen C. Reif 《Molecular breeding : new strategies in plant improvement》2011,28(4):647-655
Resistance to Fusarium head blight (FHB) is of great importance in wheat breeding programs in the northern hemisphere. In Europe, breeders prefer adapted germplasm as resistance donor because of high grain yield and quality demands. Our objective was to identify chromosomal regions affecting FHB resistance among 455 European soft winter wheat (Triticum aestivum L.) lines using a genome-wide association mapping approach and to analyze the importance of epistatic interactions. All entries were evaluated for FHB resistance by inoculation in two environments and several ratings. Wheat was genotyped by 115 simple sequence repeat markers randomly distributed across the genome and two allele-specific markers for Rht-B1 and Rht-D1 genes. The genome-wide scan revealed nine significant (P < 0.05) marker–phenotype associations on seven chromosomes including dwarfing gene Rht-D1. Using a Bonferroni–Holm correction, three significant associations remained on chromosomes 1B, 1D, and 2D. The proportion of the genotypic variance explained simultaneously by individual markers was 36% and increased to 50% when two digenic epistatic interactions were considered, one of them associated with Rht-B1. In conclusion, new genomic regions on chromosomes 1D and 3A could be found for FHB resistance in European wheat and the effect of epistatic interactions was substantial. 相似文献
19.
del Blanco IA Frohberg RC Stack RW Berzonsky WA Kianian SF 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,106(6):1027-1031
During the past decade Fusarium head blight (FHB) caused by Fusarium graminearum Schwabe has resulted in severe grain yield and quality losses of wheat (Triticum aestivum L.) in the Northern Great Plains of the U.S. Given the complexity of breeding for FHB resistance, molecular markers associated with this trait will be valuable in accelerating efforts to breed resistant cultivars. The objective of this study was to identify molecular markers linked to quantitative trait loci (QTL) for FHB resistance in wheat using a set of lines obtained by several cycles of crossing to North Dakota adapted genotypes, which derived their resistance from cv. Sumai 3. Microsatellite markers spanning the wheat genome were used to screen parents and derived lines. Polymorphisms for parental alleles were compared to disease scores for Type II resistance. The probability of linkage between markers and introgressed resistance genes was calculated using a binomial probability formula based on the assumption that a molecular marker at a specific distance from the introgressed gene, in a near-isogenic line (NIL), will carry the donor-parent allele as a function of the distance between marker and gene and the number of backcrosses/selfs performed in deriving the NIL. Microsatellite loci Xgwm533 and Xgwm274 were significantly associated with QTL for FHB resistance. 相似文献
20.
Yu JB Bai GH Cai SB Ban T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(2):308-320
The major quantitative trait locus (QTL) on 3BS from Sumai 3 and its derivatives has been used as a major source of resistance to Fusarium head blight (FHB) worldwide, but resistance genes from other sources are necessary to avoid complete dependence on a single source of resistance. Fifty-nine Asian wheat landraces and cultivars differing in the levels of FHB resistance were evaluated for type II FHB resistance and for genetic diversity on the basis of amplified fragment length polymorphism (AFLP) and simple sequence repeats (SSRs). Genetic relationships among these wheat accessions estimated by cluster analysis of molecular marker data were consistent with their geographic distribution and pedigrees. Chinese resistant landraces had broader genetic diversity than that of accessions from southwestern Japan. The haplotype pattern of the SSR markers that linked to FHB resistance quantitative trait loci (QTLs) on chromosomes 3BS, 5AS and 6BS of Sumai 3 suggested that only a few lines derived from Sumai 3 may carry all the putative QTLs from Sumai 3. About half of the accessions might have one or two FHB resistance QTLs from Sumai 3. Some accessions with a high level of resistance, may carry different FHB resistance loci or alleles from those in Sumai 3, and are worth further investigation. SSR data also clearly suggested that FHB resistance QTLs on 3BS, 5AS, and 6BS of Sumai 3 were derived from Chinese landrace Taiwan Xiaomai. 相似文献