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1.
The fungus Gnomonia comari, causal agent of strawberry leaf blotch, was inoculated at the crown of young axenized strawberry plants growing in sterilized sand. Only the roots were colonized, and the infection was symptomless. When the fungus colonized the roots in the presence of the root lesion nematode Pratylenchus penetrans, the plants were extremely stunted and their root system was necrotic. Fungal conidiospores were found attached to the cuticle of nematodes extracted from soil inoculated with the two pathogens. These findings indicate that P. penetrans could transport conidiospores through soil. 相似文献
2.
The pathogenicity and interactions of Meloidogyne naasi, Pratylenchus penetrans, and Tylenchorhynchus agri on ''Toronto C-15'' creeping bentgrass, Agrostis palustris, was studied in a long-term greenhouse experiment. Based on dry weights of roots and clippings, M. naasi alone and in all combinations with P. penetrans and T. agri was highly pathogenic to creeping bentgrass. P. penetrans and T. agri alone and in combination inhibited root growth but adversely affected top growth only when the two were co-inoculated. In combination, the effects of each species on top growth were additive, with M. naasi the dominant pathogen. Creeping bentgrass was an excellent host for M. naasi and T. agri, but a poor host for P. penetrans. T. agri inhibited population increase of M. naasi, indicating nematode-nematode competition, but neither T. agr/ nor P. penetrans was affected by any of the combinations. 相似文献
3.
We evaluated the ability of the nematode-pathogenic fungus Hirsutella rhossiliensis (Deuteromycotina: Hyphomycetes) to reduce root penetration and population increase of Pratylenchus penetrans on potato. Experiments were conducted at 24 C in a growth chamber. When nematodes were placed on the soil surface 8 cm from a 14-day-old potato cutting, the fungus decreased the number entering roots by 25%. To determine the effect of the fungus on population increase after the nematodes entered roots, we transplanted potato cuttings infected with P. penetrans into Hirsutella-infested and uninfested soil. After 60 days, the total number of nematodes (roots and soil) was 20 ± 4% lower in Hirsutella-infested than in uninfested soil. 相似文献
4.
G. M. GURR 《The Annals of applied biology》1992,121(1):167-173
Seven trials conducted over four years on sites naturally infested with the white potato cyst nematode established that potato clones bred for resistance to Globodera pallida allowed significantly less nematode multiplication than conventional cultivars under field conditions. Nematode multiplication was inversely related to initial infestation level. The nematicide, aldicarb, significantly reduced nematode multiplication. However, nematode multiplication on nematicide treated susceptible cultivars was greater than on untreated partially resistant clones, indicating that resistance may offer more effective control of G. pallida than chemical treatment. Integration of host plant resistance and nematicide treatment is discussed. 相似文献
5.
J. A. LaMondia 《Journal of nematology》1999,31(4):418-423
Microplot and small field-plot experiments were conducted to determine the effects of Pratylenchus penetrans on strawberry yield over several seasons and to evaluate the effects of nematode control on strawberry vigor and yield. Pratylenchus penetrans alone or in combination with the black root rot pathogen, Rhizoctonia fragariae, reduced strawberry yield in microplots over time. There were no differences in effects on yield among R. fragariae anastomosis groups A, G, or I. The interaction of the two pathogens appeared to be additive rather than synergistic. In field plots infested with P. penetrans alone, plant vigor and yield were increased by the application of carbofuran and fenamiphos nematicides. Nematode control was transitory, as P. penetrans populations were initially suppressed but were not different in samples taken 10 months after treatment. These data highlight the error in associating causality between plant damage and nematode populations based on a correlation of root disease with nematode diagnostic assays from severely diseased plants. These findings may help to explain how nematode numbers can sometimes be higher in healthy plants than in severely diseased plants that lack sufficient roots to maintain nematode populations. Because nematode populations from up to a year before harvest are better correlated with berry yield, preplant nematode diagnostic assays taken a year in advance of harvest may be superior in predicting damage to perennial strawberry yield. 相似文献
6.
Pratylenchus vulnus suppressed the growth of sour orange seedlings in greenhouse experiments. Growth retardation (in height, in trunk diameter, and in dry top and root weights) was observed in inoculated plants growing in two soil types. Population density, 13 months after inoculation, averaged more than 1,000 specimens/gm of fresh root. Anatomical studies showed that P. vulnus prefers to attack the cortex and causes cavities among the cortical cells. 相似文献
7.
C. B. Willis 《Journal of nematology》1972,4(4):291-295
''Vernal'' alfalfa was grown for 30 weeks in nematode-free soil and in soil infested with Pratylenchus penetrans. Charlottetown fine sandy loam soil was used at its pH of 4.4 and at adjusted reactions of 5.2, 6.4 and 7.3. Nematode reproduction was significantly greater at pit 5.2 and 6.4 and was not related to alfalfa root production over the full pH range studied. A significant nematode infestation X soil pit interaction on forage yield was recorded. Nematode infestation significantly decreased forage yields at ptt 5.2 and 6.4 but not at pH 4.4 and 7.3. 相似文献
8.
利用变性梯度凝胶电泳(PCR-DGGE)技术,研究了放牧对贝加尔针茅(Stipa baicalensis)草原土壤细菌群落结构的影响。结果表明,放牧条件下,贝加尔针茅草原0~20cm土壤中细菌类群包括变形菌门(Proteobacteria)的α、β、γ、δ变形菌纲,放线菌门(Actinobacteria)、芽单胞菌门(Gemmatimonadetes)、绿弯菌门(Chloroflexi)、厚壁菌门(Firmicutes)、酸杆菌门(Acidobacteria)、疣微菌门(Verrucomicrobia)、拟杆菌门(Bacteroidetes)、硝化螺旋菌门(Nitrospirae)等,其中变形菌门、放线菌门属优势类群,疣微菌门细菌是中度放牧样地仅有的类群;典型对应分析表明,土壤细菌群落与放牧强度、土壤理化性状密切相关。轻度放牧样地土壤细菌群落与有机碳、C/N比有较为密切的关系,中度放牧样地土壤细菌群落则与全N有较密切的关系,重度放牧样地土壤细菌群落与土壤pH有关。 相似文献
9.
Forge Thomas Muehlchen Andrea Hackenberg Clemens Neilsen Gerry Vrain Thierry 《Plant and Soil》2001,236(2):185-196
Six species of arbuscular mycorrhizal (AM) fungi (Glomus aggregatum, G. clarum, G. etunicatum, G. intraradices, G. mosseae and G. versiforme) were evaluated, in three greenhouse experiments, for their effects on reproduction of the root-lesion nematode, Pratylenchus penetrans, and growth of Ottawa 3 apple rootstock. Glomus mosseae increased total dry weights of nematode-inoculated and non-inoculated rootstock in all three greenhouse experiments, and G. intraradices increased dry weights in two of three greenhouse experiments. Plants inoculated with G. mosseae generally supported fewer P. penetrans per gram of root than plants inoculated with other AM fungi, but did not differ significantly from the controls in any greenhouse experiment. Colonization of roots by AM fungi was reduced by P. penetrans at initial inoculum densities greater than 250 nematodes/L soil. In field trials, preplant inoculation with either G. intraradices or G. mosseae increased rootstock growth and leaf concentrations of P, Mg, Zn and Cu in fumigated plots but not in non-fumigated plots, indicating that colonization by native AM fungi in non-fumigated plots may have been sufficient for adequate nutrient acquisition. The abundance of vesicles and arbuscules was greater in roots of plants inoculated with AM fungi before planting than in roots of non-inoculated plants, in both fumigated and non-fumigated plots. P. penetrans per gram of root and per 50 ml soil were significantly lower for G. mosseae- inoculated plants than for non-inoculated plants in fumigated soil but not in non-fumigated soil. 相似文献
10.
Haddish Melakeberhan 《Journal of nematology》1998,30(1):93-99
The pathogenicity of Heterodera glycines, Meloidogyne incognita, and Pratylenchus penetrans on H. glycines-resistant ''Bryan,'' tolerant-susceptible ''G88-20092,'' and intolerant-susceptible ''Tracy M'' soybean cultivars was tested using plants grown in 800 cm³ of soil in 15-cm-diam. clay pots in three greenhouse experiments. Plants were inoculated with 0, 1,000, 3,000, or 9,000 H. glycines race 3 or M. incognita eggs, or vermiform stages of P. penetrans/pot. Forty days after inoculation, nmnbers of all three nematodes, except H. glycines on Bryan, generally increased with increasing inoculum levels in Experiment I. Heterodera glycines and M. incognita significantly decreased growth only of Tracy M. At 45 and 57 days after inoculation with 6,000 individuals/pot in experiments II and III, respectively, significantly more P. penetrans and M. incognita than H. glycines were found on Bryan. However, H. glycines and M. incognita population densities were greater than P. penetrans on G88-20092 and Tracy M. Growth of Tracy M infected by H. glycines and M. incognita and growth of G88-20092 infected by M. incognita decreased in Experiment III. Pratylenchus penetrans did not affect plant growth. Reduction in plant growth differed according to the particular nematode species and cultivar, indicating that nematodes other than the species for which resistance is targeted can have different effects on cultivars of the same crop species. 相似文献
11.
Ulrich Zunke 《Journal of nematology》1990,22(3):309-320
The endoparasitic behavior of Pratylenchus penetrans was examined using video-enhanced contrast microscopy to observe living nematodes inside root tissue. Feeding behavior could be separated into phases of probing, cell penetration by the stytet, salivation, and food ingestion for brief and extended periods. After cell penetration, a small "salivation zone" was formed around the stylet tip. No feeding tubes were observed. Feeding and migration were interrupted by rest phases when a nematode became characteristically coiled inside a cell. Tissue damage was caused primarily by migration and extended feeding periods. Aspects of egg laying and molting are also described. 相似文献
12.
Th. H. A. Olthof 《Journal of nematology》1987,19(4):424-430
Replicated trials were conducted near Alliston, Ontario, in 1983 and 1984 to evaluate the efficacy of five chemical treatments in controlling the root-lesion nematode Pratylenchus penetrans on potato, Solanum tuberosum cv. Russet Burbank. The fumigants Vorlex, at 55 liters product/ha, and Telone II B, at 75 liters product/ha, were more effective in suppressing high initial population densities of 18,320 and 50,880 P. penetrans/kg soil in 1983 and 1984, respectively, than single applications of the systemic pesticides Temik 10 G at 22 kg product/ha, Vydate L at 18 and 9 liters product/ha, and Furadan 10 G at 33 kg product/ha. The combination of Vorlex + Temik resulted in greatest nematode suppression and lowest populations at harvest. In 1983, marketable tuber yield (> 7 cm) in the Vorlex + Temik plots was 20.7 t/ha, compared to 4.7 t for the untreated check. Vorlex alone and Telone II B plots yielded 17.3 and 15.9 t/ha, respectively; Temik with 7.5 t also yielded better than the check. Vydate and Furadan did not influence yields significantly. Total yields differed from the check in all treatments except with Furadan. In 1984, marketable yields ranged from 15.5 t/ha for the Vorlex + Temik treatment to 11.2 for the untreated check, but the differences were not statistically significant. Total yields, however, were significantly increased by the fumigants. The difference in response to chemical treatment in the 2 years was attributed to greater heat stress and lack of supplementary irrigation in 1983. 相似文献
13.
土壤是生态系统中物质循环和能量转化的重要场所,转基因作物外源基因对土壤非靶标生物的影响已经引起人们的广泛关注.我国转植酸酶基因玉米(即自交系BVLA430101)于2009年9月27日获得了生物安全证书,该转植酸酶基因玉米可提高饲料利用效率,减少动物粪便造成的环境污染.本文以转植酸酶基因玉米(简称转基因玉米)和常规对照亲本玉米(简称对照玉米)为试验材料,通过大田试验研究了转基因玉米和对照玉米种植对土壤线虫群落组成与生态指标的影响.结果表明: 转基因玉米和对照玉米大田土壤分别分离出29个属和26个属的线虫.与对照玉米相比,转基因玉米种植田食细菌线虫相对多度与数量、捕/杂食线虫数量和土壤线虫总数,以及群落多样性指数等都有升高的趋势,而植食线虫相对多度与线虫总成熟度指数呈降低趋势.重复测量方差分析表明,整个生长季节内转基因玉米与对照玉米田间不同营养类群土壤线虫相对多度与数量及生态指标均无显著差异;而T检验分析表明,玉米乳熟期转基因玉米田食细菌线虫、捕/杂食线虫数量和土壤线虫总数显著高于对照玉米田,这可能与乳熟期转基因玉米田土壤总氮含量显著升高有关. 相似文献
14.
Plants of potato (Solanum tuberosum) cultivars Katahdin and Superior were inoculated with 0, 1,500, or 15,000 Pratylenchus penetrans. Transpiration, measured in the greenhouse with a porometer after 56 days of growth, was not significantly different among nematode inoculum levels or between cultivars. The rate of xylem exudation from decapitated root systems of Katahdin plants inoculated with 1,500 or 15,000 P. penetrans and Superior plants inoculated with 15,000 P. penetrans was lower than from noninoculated plants. Root weight of Katahdin and Superior was not affected by P. penetrans inoculum level. Transpiration of plants inoculated with 0, 500, 5,000 or 50,000 P. penetrans was recorded weekly from 14 to 56 days after planting. No consistent effects of nematode inoculum density on transpiration rate were observed. Root hydraulic conductivity was lower in Katahdin plants inoculated with 266 P. penetrans per plant and in Chippewa with 5,081 per plant than in noninoculated plants. Nematodes reduced leaf area of Superior, Chippewa, and Katahdin and root dry weight of Chippewa but had no effect on growth of Hudson, Onaway, or Russet Burbank plants. Assessing nematode effects on root hydraulic conductivity may provide a measure of the tolerance of potato cultivars to nematodes. 相似文献
15.
A greater percentage of females than juveniles or males of P. penetrans penetrated celery roots grown in infested soil at 5, 18, or 30 C; the difference was greatest at 5 C. The time of initial penetration of alfalfa seedlings inoculated with single nematodes on water agar varied with temperature. Females penetrated the seedlings earlier and over a wider range of temperatures than did males or juveniles. The rate of penetration was highest for females. After initial penetration, the penetration rate decreased with time. At 13-28 C, approximately 80% of roots were penetrated by females and only 25-30% by males and juveniles by the end of the experiment. 相似文献
16.
Four similar growth chamber experiments were conducted to test the hypothesis that the initial population density (Pi) of Pratylenchus penetrans influences the severity of interactive effects of P. penetrans and Verticillium dahliae on shoot growth, photosynthesis, and tuber yield of Russet Burbank potato. In each experiment, three population densities of P. penetrans with and without concomitant inoculation with V. dahliae were compared with nematode-free controls. The three specific Pi of JR penetrans tested varied from experiment to experiment but fell in the ranges 0.8-2.5, 1.8-3.9, 2.1-8.8, and 7.5-32.4 nematodes/cm³ soil. Inoculum of V. dahliaewas mixed into soil, and the assayed density was 5.4 propagules/gram dry soil. Plants were grown 60 to 80 days in a controlled environment. Plant growth parameters in two experiments indicated significant interactions between P. penetrans and V. dahliae. In the absence of V. dahliae, P. penetrans did not reduce plant growth and tuber yield below that of the nematode-free control or did so only at the highest one or two population densities tested. In the presence of K dahliae, the lowest population density significantly reduced shoot weight and photosynthesis in three and four experiments, respectively. Higher densities had no additional effect on shoot weight and caused additional reductions in photosynthesis in only one experiment. Population densities of 0.8 and 7.5 nematodes/cm³ soil reduced tuber yield by 51% and 45%, whereas higher densities had no effect or a 15% additional effect, respectively. These data indicate that interactive effects between P. penetrans and V. dahliae on Russet Burbank potato are manifested at P. penetrans population densities less than 1 nematode/cm³ soil and that the nematode population density must be substantially higher before additional effects are apparent. 相似文献
17.
紫色土区不同秸秆还田量对土壤线虫群落的影响 总被引:2,自引:0,他引:2
为了研究不同秸秆还田量对紫色土区土壤线虫群落的影响, 作者于2011年5月和9月对川中丘陵区的100% (RMW1)、50%(RMW2)、30%(RMW3)和空白对照(CK)4种秸秆还田量的农田土壤线虫进行了调查。结果发现, 随着秸秆还田量的下降, 土壤线虫的群落结构有一定变化。总体上, 群落密度、食真菌类群和捕食-杂食类群的密度呈波动增加; 食细菌类群的密度持续增加; 植物寄生类群密度、Shannon-Wiener多样性指数(H')、植物寄生成熟指数(PPI)和瓦斯乐斯卡指数(WI)呈先增加后下降的变化趋势, 且多样性指数在秸秆还田量为30-50%之间最高; 类群数、自由生活成熟指数(MI)、线虫通路比值(CR)则先下降后增加; 所有变化均不显著(P > 0.05)。土壤速效钾含量随秸秆还田量的增加显著提高(P < 0.05), 且与线虫群落多样性指数呈显著负相关(P < 0.05)。研究结果表明, 在紫色土区秸秆还田主要是通过改变土壤钾含量对土壤线虫群落产生影响, 且秸秆还田量在30-50%之间时有利于维持线虫群落多样性。 相似文献
18.
The effect of slow-release urea on soil nematode community structure was investigated in a soybean field in northeast China.Three treatments,no urea (CK),conventional urea (U) and slow-release urea (SRU),were arranged in a completely random design.The results show that the abundance of total nematodes was significantly higher in SRU than in CK and U.Significant differences in the abundance of bacterivores with colonizer-persister (cp) values 2-3,fungivores with cp 2 and herbivores with cp 3 were found among different treatments.Forty-one genera were identified,of which Acrobeloides,Aphelenchus and Heterodera were dominant.Soil nematode guilds and genera exhibited different responses to slow-release urea.The most trophic groups and genera had greater abundances in SRU than in CK and U.Slow-release urea had a positive effect on soil nematode community structure. 相似文献
19.
The effect of slow-release urea on soil nematode community structure was investigated in a soybean field in northeast China.
Three treatments, no urea (CK), conventional urea (U) and slow-release urea (SRU), were arranged in a completely random design.
The results show that the abundance of total nematodes was significantly higher in SRU than in CK and U. Significant differences
in the abundance of bacterivores with colonizer-persister (cp) values 2–3, fungivores with cp 2 and herbivores with cp 3 were
found among different treatments. Forty-one genera were identified, of which Acrobeloides, Aphelenchus and Heterodera were dominant. Soil nematode guilds and genera exhibited different responses to slow-release urea. The most trophic groups
and genera had greater abundances in SRU than in CK and U. Slow-release urea had a positive effect on soil nematode community
structure. 相似文献
20.
In a petri-dish study, development of the nematode Pratylenchus neglectus was observed every 4 days, and stage-specific development times were estimated, using a parameter estimation algorithm for a distributed-delay population model. The lower threshold temperature for development of a population of P. neglectus was 7.75 C. Temperatures above 25 C were unfavorable for this population on barley. Total numbers of P. neglectus in barley roots and associated soil in pots were greatest at 25 C and lower at temperatures above and below that level. There was no change in nematode numbers per gram of root as temperature increased between 24 C and 32 C because root weights decreased at higher temperatures. Restricted root mass may contribute to the lower total nematode population levels at higher temperature. Maximum number of nematodes moved through a 2-cm layer of sand on a Baermann funnel at about 20 C; lowest number of nematodes moved at 10 C and 30 C. 相似文献