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Spatial analysis of lipid metabolites and expressed genes reveals tissue‐specific heterogeneity of lipid metabolism in high‐ and low‐oil Brassica napus L. seeds 下载免费PDF全文
Shaoping Lu Drew Sturtevant Mina Aziz Cheng Jin Qing Li Kent D. Chapman Liang Guo 《The Plant journal : for cell and molecular biology》2018,94(6):915-932
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Carly D. Kenkel Aurelie Moya Julia Strahl Craig Humphrey Line K. Bay 《Global Change Biology》2018,24(1):158-171
Little is known about the potential for acclimatization or adaptation of corals to ocean acidification and even less about the molecular mechanisms underpinning these processes. Here, we examine global gene expression patterns in corals and their intracellular algal symbionts from two replicate population pairs in Papua New Guinea that have undergone long‐term acclimatization to natural variation in pCO2. In the coral host, only 61 genes were differentially expressed in response to pCO2 environment, but the pattern of change was highly consistent between replicate populations, likely reflecting the core expression homeostasis response to ocean acidification. Functional annotations highlight lipid metabolism and a change in the stress response capacity of corals as key parts of this process. Specifically, constitutive downregulation of molecular chaperones was observed, which may impact response to combined climate change‐related stressors. Elevated CO2 has been hypothesized to benefit photosynthetic organisms but expression changes of in hospite Symbiodinium in response to acidification were greater and less consistent among reef populations. This population‐specific response suggests hosts may need to adapt not only to an acidified environment, but also to changes in their Symbiodinium populations that may not be consistent among environments, adding another challenging dimension to the physiological process of coping with climate change. 相似文献
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Susceptibility to disinfectants in antimicrobial‐resistant and ‐susceptible isolates of Escherichia coli,Enterococcus faecalis and Enterococcus faecium from poultry–ESBL/AmpC‐phenotype of E. coli is not associated with resistance to a quaternary ammonium compound,DDAC 下载免费PDF全文
N. Wieland J. Boss S. Lettmann B. Fritz K. Schwaiger J. Bauer C.S. Hölzel 《Journal of applied microbiology》2017,122(6):1508-1517
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Abeer Hammoudi Fei Song Karen R. Reed Rosalind E. Jenkins Valerie S. Meniel Alastair J.M. Watson D. Mark Pritchard Alan R. Clarke John R. Jenkins 《Biochemical and biophysical research communications》2013
Colorectal cancer (CRC) is the fourth most common cause of cancer-related death worldwide. Accurate non-invasive screening for CRC would greatly enhance a population’s health. Adenomatous polyposis coli (Apc) gene mutations commonly occur in human colorectal adenomas and carcinomas, leading to Wnt signalling pathway activation. Acute conditional transgenic deletion of Apc in murine intestinal epithelium (AhCre+Apcfl/fl) causes phenotypic changes similar to those found during colorectal tumourigenesis. This study comprised a proteomic analysis of murine small intestinal epithelial cells following acute Apc deletion to identify proteins that show altered expression during human colorectal carcinogenesis, thus identifying proteins that may prove clinically useful as blood/serum biomarkers of colorectal neoplasia. Eighty-one proteins showed significantly increased expression following iTRAQ analysis, and validation of nine of these by Ingenuity Pathaway Analysis showed they could be detected in blood or serum. Expression was assessed in AhCre+Apcfl/fl small intestinal epithelium by immunohistochemistry, western blot and quantitative real-time PCR; increased nucelolin concentrations were also detected in the serum of AhCre+Apcfl/fl and ApcMin/+ mice by ELISA. Six proteins; heat shock 60 kDa protein 1, Nucleolin, Prohibitin, Cytokeratin 18, Ribosomal protein L6 and DEAD (Asp-Glu-Ala-Asp) box polypeptide 5,were selected for further investigation. Increased expression of 4 of these was confirmed in human CRC by qPCR. In conclusion, several novel candidate biomarkers have been identified from analysis of transgenic mice in which the Apc gene was deleted in the intestinal epithelium that also showed increased expression in human CRC. Some of these warrant further investigation as potential serum-based biomarkers of human CRC. 相似文献