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1.
Cauliflower (Brassica oleracea L. botrytis) and broccoli (Brassicaoleracea L. italica) plants were grown in large pots in growthchambers for a range of temperatures (mean air temperaturesfrom 7.0-25.3 C) and irradi-ances (from 9.3-50.8 mol m2d1 or 4.7-25.4 MJ m2 d1). The extinctioncoefficient for PAR decreased with plant size reaching a valueof 0.55 in cauliflower and 0.45 in broccoli at plant leaf areasof 0.235 m2 and 0.227 m2, respectively. The leaf area expansionrate was unaffected by irradiance when compared at identicalleaf surface temperatures. The response of expansion rate tosurface temperature was fitted to a broken stick model witha base temperature of 0.7C and an optimum temperatureof 21.0C. The radiation conversion coefficient increased withair temperature below 13.8C and remained constant above this.The estimated radiation conversion coefficient above 13.8Cand for a PPFD of 20 mol m2 d1 was 0.77 g mol1in cauliflower and 0.87 g mol1 in broccoli. The radiationconversion coefficient declined with increasing irradiance levelfrom a maximum of 1.89 g mol1 at near nil irradiancein cauliflower. Key words: Leaf area, dry matter, radiation use efficiency, extinction coefficient 相似文献
2.
The marine diatoms Lauderia annulata and Synedra planctonicaharvested during exponential growth were exposed to differentdoses of u.v.-B (286, 439 and 710 J m2 d1) for2 days. Uptake of 15N-nitrate was estimated before, during andafter u.v.-B radiation over 2 days. Exposure to high levelsof u.v.-B (710 J m2 d1) caused irreversible damageat the second daily irradiance. Lauderia cells were less affectedby u.v.-B stress than Synedra cells. 15N-nitrate uptake wasreduced under u.v.-B irradiance but could be reactivated within1 day following exposure to a low dose (286 J m2 d1).Higher levels of u.v.-B radiation (710 J m2 d1)led to irreversible damage. The pattern of 15N-incorporationinto several amino acids of Lauderia varied after 2 days ofu.v.-B radiation. 15N enrichment of glutamine increased markedlyafter u.v.-B stress (717 J m2 d1) whereas I5Nexcess of aspartic acid was significantly reduced. Results arediscussed with reference to the u.v.-B damage of the nitratetransport system. 相似文献
3.
Zoea I larvae of the brown shrimp Crangon crangon (Decapoda)were exposed to varying levels of UV radiation in a sunshinesimulator. Short-term exposures (08 h)were used to determine the highest UV dose with no significanteffect (NOEC; defined by limit of detection) and the lethaldose of 10 and 50% mortality (LD10 and LD50). Crangon crangonshowed a relatively high sensitivity to UVB radiation (NOEC= 10 kJ m2, LD10 = 15 kJ m2, LD50 = 24 kJ m2)compared to other crust-acean species. LD values (19971998)showed no adaptation to seasonal light regimes. Long-termexposures (010 days) were carried out to assessthe range where the law of reciprocity is valid.The larvae were exposed to UV levels of 0.2, 0.4 and 0.7 J m2for appropriate time intervals, always cumulating in a sublethaldose of 5 kJ m2 day1. Results reflect a possiblethreshold (0.20.4 J m2 UVB) in the effect of thedifferent UVB doses used; thus, a proportional relationshipof intensity and exposure time can only be shown at UVB levelsabove this threshold intensity. 相似文献
4.
The Response of Bean Plants to UV-B Radiation Under Different Irradiances of Background Visible Light 总被引:14,自引:1,他引:13
Plants of Phaseolus vulgaris L. (cv. Stella) were grown in controlledconditions under three different irradiances of visible lightwith or without UV-B (280320nm) radiation. The biologicallyeffective UV-B radiation (UV-BBE) was 6.17 kJ m2 d1,and simulated a c. 5% decrease in stratospheric ozone at 55.7?N,13.4?E. The photon flux densities of the photosyntheticallyactive radiation (PAR, 400700 nm) were either 700 µmolm21 (HL), 500, µmol m2 s1(ML) or 230 µmol m2 s1 PAR (LL). Under highlight (HL) conditions plus UV-B radiation, bean plants appearedmost resistant to the enhanced levels of UV-B radiation, andresponded only by increasing leaf thickness by c. 18%. A smallincrease in UV screening pigments was also observed. Both thelower irradiances (ML and LL) increased the sensitivity of theplants to UV-B radiation. Changes in leaf structure were alsoobserved. Photosystem II was inhibited under ML and LL togetherwith UV-B radiation, as determined by Chi fluorescence inductionand calculation of the fluorescence half-rise times. Leaf reflectivitymeasurements showed that the amount of PAR able to penetrateleaves of UV-B treated plants was reduced, and that a possiblecorrelation may exist between the reduced PAR levels, loss ofChi and lowered photosynthetic activity, especially for LL +UV-Bgrown plants, where surface reflection from leaves was highest.Changes in leaf chlorophyll content were mostly confined toplants grown under LL + UV-B, where a decrease of c. 20% wasfound. With regard to protective pigments (the carotenoids andUV screening pigments) plants subjected to different visiblelight conditions responded differently. Among the growth parametersmeasured, there was a substantial decrease in leaf area, particularlyunder LL + UV-B (c. 47% relative to controls), where leaf dryweight was also reduced by c. 25%. Key words: Chlorophyll fluorescence induction, bean, flavonoids, Phaseolus vulgaris, reflectance, UV-B radiation 相似文献
5.
LARSON KIRK D.; SCHAFFER BRUCE; DAVIES FREDERICK S. 《Journal of experimental botany》1993,44(3):665-671
A system was developed to test the effects of floodwater O2concentration on ethylene evolution and stem lenticel hypertrophy,and the effects of exogenous ethylene on stem lenticel hypertrophyin mango (Mangifera indica L.) trees. Dissolved O2 concentrationsof 17x109 m3 m3 generally resulted in hypertrophyof stem lenticels within about 6 d of flooding, whereas floodwaterO2 concentrations of 1315 x 109 m3 m3 delayedhypertrophy until about day 9. After 14d of flooding, therewere more than twice the number of hypertrophied lenticels pertree with floodwater O2 concentrations of 17 x 109m3 m3 than with floodwater O2 concentrations of 15 x109 m3 m3. Ethylene evolution from stem tissueimmediately above the floodline increased 4- to 8-fold in treesexposed to floodwater O2 concentrations of 12 x 109m3 m3, increased 2-fold for trees exposed to floodwaterO2 concentrations of 67 x 109 m3 m3, butremained constant with floodwater O2 concentrations of 1315x 109 m3 m3. Plants maintained in highly oxygenatedfloodwater (1315 x 109 m3 m3), and givenexogenous ethylene developed many hypertrophied lenticels, whereasplants in highly oxygenated water and not given ethylene developedfewer or nohypertrophied lenticels. These data suggest thatethylene plays a role in promotion of stem lenticel hypertrophyin flooded mango trees, and that floodwater dissolved oxygenconcentration can regulate stem lenticel hypertrophy and ethyleneevolution in this species. Key words: Flooding, hypoxia, hypertrophic cell swelling 相似文献
6.
The rates of CO2 assimilation by potted spray carnation plants(cv. Cerise Royalette) were determined over a wide range oflight intensities (45450 W m2 PAR), CO2 concentrations(2003100 vpm), and leaf temperatures (535 °C).Assimilation rates varied with these factors in a way similarto the response of single leaves of other temperate crops, althoughthe absolute values were lower. The optimal temperature forCO2 assimilation was between 5 and 10 °C at 45 W m2PAR but it increased progressively with increasing light intensityand CO2 concentration up to 27 °C at 450 W m2 PARand 3100 vpm CO2 as expressed by the equation TOpt = 6.47-h 2.336 In G + 0.031951 where C is CO2 concentration in vpmand I is photo-synthetically active radiation in W m2.CO2 enrichment also increased stomatal resistance, especiallyat high light intensities. The influence of these results on optimalization of temperaturesand CO2 concentrations for carnation crops subjected to dailylight variation, and the discrepancy between optimal temperaturesfor growth and net photosynthesis, are discussed briefly 相似文献
7.
Loliun perenne L. (cv.S. 23) was grown on vermiculite in winterin a heated greenhouse for 8 weeks under factorial combinationsof two potassium regimes (nominally 6 parts/106 and 156 parts/106in Hewitt's solution) and three densities of artificially supplementedvisible radiation flux (36.1, 7.3, and 2.2 W m2). Growthand potassium uptake were studied through the calculation ofvarious growth functions from fitted curves. There was little effect of potassium treatment but the experimentalmaterial responded markedly to light. Leaf-area ratio in thethree treatments showed extreme plasticity in increasing from23 x 102 through 6 x 102 to 89 x102 m2 g1 as light intensity decreased. Correspondingdecreases in unit leaf rate, however, caused over-all reductionsin relative growth rate. Specific absorption rates for potassium (AK, dry-weight basis)were strongly reduced at the lower light intensities but alsodisplayed complex ontogenetic drifts. Values of the allometricconstant, k (the ratio of root and shoot relative growth rates),decreased from c. 0.7 at 36.1 W m2 through c. 0.3 at7.3 W m2 to a value not significantly different fromzero (P < 0.05) at 2.2 W m2. In material grown under the two higher light intensities a constantinverse relationship was found between the mass ratio of rootand shoot and the corresponding activity ratio. The resultsconform to this model: Mass ratio = 0.001+45.0 (1/activityratio) where activity ratio is expressed as specific absorptionrate for potassium (in µg g root1 h1)/unitshoot rate (rate of increase of whole-plant dry weight per unitshoot dry weight, in mg g shoot1 h1). The implicationsof this relationship are discussed. 相似文献
8.
Growth and Nitrogen Fixation of Phaseolus vulgaris L. at Two Irradiances II. Nitrogen Fixation 总被引:1,自引:0,他引:1
Phaseolus vulgaris cv. Glamis plants grown at 7 and 28 W m228 W m2 in controlled environment cabinets showed copiousnodulation and high levels of acetylene reducing activity. Earlydifferences in nodulation were apparent before differences inphotosynthesis and were attributed to an effect of far-red lighton nodule development. Total plant nitrogen content was greater at 28 W m2 thanat 7 W 2 but nitrogen content as a percentage of d. wtwas greater at the lower irradiance level. Total acetylene reducing activity (nmol. min1 root1)was greater at 28 W m2 than at 7 W 2, but therewas no irradiance effect on specific activity (nmol. min1g d. wt of pink nodules1 or nmol. min1 pink nodule1). Transfer of 40-day-old plants from 7 W m2 to 28 W m2resulted in increased nodulesize(due toincreased size of infectedcells), accompanied by increased total, but not specific, acetylenereducing activity. Transfer of plants from 28 W m2 to 7 W m2 resultedin a fall of total acetylene reducing activity within 24 h,and senescence of large nodules. Specific acetylene reducingactivity was unaffected The results are interpreted as an effect of light on the productionof nitrogen fixing tissue, rather than on nitrogenase activity. 相似文献
9.
The Relationship between Sucrose and Starch during 'Dark' Export from Leaves of Uniculm Barley 总被引:7,自引:0,他引:7
Uniculm barley plants were grown in 8 h photoperiods at a quantumflux density of 655 µE m2 s1. Groups ofplants were transferred to four different light environmentsfor one 8 h photoperiod (106, 270, 665, and 975 µE m2s1) and harvested at intervals throughout the succeedingdark period for subsequent carbohydrate analysis of the youngestmature leaf. Sucrose was the predominant carbohydrate in the leaves (attaininga level of c. 100 mg dm2 after 8 h at 975 µE m2s1) but starch was also of significance (20 mg dm2after 8 h at 975 µE m2 s1). During the dark period, following a photoperiod at the threehighest light levels (270, 665, and 975 µE m2 s1),sucrose was exported first while the starch level remained fairlyconstant. When the-sucrose level fell to 1520 mg dm2starch degradation began. This critical sucrose level was reachedearlier in those plants subjected to lower quantum flux densitiesduring the preceding photoperiod. The delay in the remobilizationof starch suggests an important regulatory mechanism which maybe dependent upon the sucrose level. At 106 µE m2s1 the sucrose level rose to only 10 mg dm2. Herethere was no discernible delay in the depletion of sucrose orstarch. 相似文献
10.
CLEMENT C. R.; HOPPER M. J.; JONES L. H. P.; LEAFE E. L. 《Journal of experimental botany》1978,29(5):1173-1183
Experiments with simulated swards of perennial ryegrass (Loliumperenne L.) grown in flowing nutrient solution with NO3- heldat 0.1 mg N I1 show that the rate of NO3- uptake wasrelated to diurnal, day-to-day, and seasonal changes in radiation.In summer the diurnal variation in NO3-uptake ranged from 25to 50 mg N m2 h1 and the day-to-day variationranged from 500 to 1500 mg N m2 d1. Mean dailyrates of uptake over 12 d periods in summer and in winter averaged908 and 44 mg N m2, respectively. The pattern of NO3-uptake followed that of CO2 flux with the maximum rate of theformer occurring 5 or 6 h after the maximum CO2 influx. Afterdefoliation, NO3- uptake was severely curtailed for 2 d concomitantwith a very small influx of CO2. Analysis of the changes thatoccurred in the rate of NO3- uptake immediately after the switchingon or off of artificial light suggests that two reversible processesmay be involved in the relation between NO3-uptake and radiation,one with a longer and the other with a shorter time constant. 相似文献
11.
Effects of photon flux density on carbon partitioning and rhizosphere carbon flow of Lolium perenne 总被引:1,自引:0,他引:1
Hodge Angela; Paterson Eric; Thornton Barry; Millard Peter; Killham Ken 《Journal of experimental botany》1997,48(10):1797-1805
The distribution and partitioning of dry matter and photoassimilateof Lolium perenne was investigated under two light regimes providingphotosynthetically active radiation of 350 µmol m2s1 (low light treatment) or 1000 µmol m2s1 (high light treatment). Plants were grown at specificgrowth conditions in either soil or sand microcosm units tofollow the subsequent release of carbon into the rhizosphereand its consequent incorporation into the microbial biomass(soil system) or recovery as exudates (sand system). The distributionof recent assimilate between the plant and root released carbonpools was determined using 14CO2 pulse-chase methodology atboth light treatments and for both sand- and soil-grown seedlings.A significant (P 相似文献
12.
Hogan K.P.; Fleck I.; Bungard R.; Cheeseman J.M.; Whitehead D. 《Journal of experimental botany》1997,48(6):1289-1297
Red beech (Nothofagus fusca (Hook. F.) Oerst.; Fagaceae) andradiata pine (Pinus radiata D. Don; Pinaceae) were grown for16 months in large open-top chambers at ambient (37 Pa) andelevated (66 Pa) atmospheric partial pressure of CO2, and incontrol plots (no chamber). Summer-time measurements showedthat photosynthetic capacity was similar at elevated CO2 (lightand CO2-saturated value of 17.2 µmol m2 s1for beech, 13.5 µmol m2 s1 for pine), plantsgrown at ambient CO2 (beech 21.0 µmol2 s1,pine 14.9 µmol m2s1) or control plants grownwithout chambers (beech 23.2 µmol m2 s1,pine 12.9 µmol m2 s1). However, the higherCO2 partial pressure had a direct effect on photosynthetic rate,such that under their respective growth conditions, photosynthesisfor the elevated CO2 treatment (measured at 70 Pa CO2 partialpressure: beech 14.1 µmol m2 s1 pine 10.3)was greater than in ambient (measured at 35 Pa CO2: beech 9.7µmol m2 s1, pine 7.0 µmol m2s1) or control plants (beech 10.8 µmol m2s1, pine 7.2 µmol m2 s1). Measurementsof chlorophyll fluorescence revealed no evidence of photodamagein any treatment for either species. The quantity of the photoprotectivexanthophyll cycle pigments and their degree of de-epoxidationat midday did not differ among treatments for either species.The photochemical efficiency of photosystem II (yield) was lowerin control plants than in chamber-grown plants, and was higherin chamber plants at ambient than at elevated CO2. These resultssuggest that at lower (ambient) CO2 partial pressure, beechplants may have dissipated excess energy by a mechanism thatdoes not involve the xanthophyll cycle pigments. Key words: Carotenoids, chlorophyll fluorescence, photosynthesis, photoinhibition, photoprotection, xanthophyll cycle 相似文献
13.
The Relationship Between Shoot Weight, Plant Density and Time During the Propagation of Four Vegetable Species 总被引:3,自引:1,他引:2
Lettuce, cauliflower, leeks and celery were grown from seedin a glasshouse at four widely-differing densities on severaloccasions over a 15-month period, under conditions of amplewater and nutrients. For each sowing there were four early harvests.The reciprocal of mean shoot dry weight was found to be linearlyrelated to plant density at any particular harvest as in theShinozakiKira equation. The reciprocal of the slope ofthis relationship, representing the ceiling yield m2was roughly proportional to mean daily radiation during theperiod of 14 d before harvest: the reciprocal of the interceptwas logarithmically related to accumulated heat units, modifiedfor daily radiation. Hence mean plant weight could be expressedas the following function of environmental variables and plantdensity: w2 = w0pep. 相似文献
14.
Effect of Photoperiod on Growth of Sugar Beet 总被引:1,自引:0,他引:1
Sugar beet grown in controlled environments were given similardaily amounts of visible radiation during three different photoperiodtreatments. Plants were given (a) 115 W m2 visible irradiancefrom fluorescent and tungsten lamps for 12 h; (b) 88 W m2of the same light for 16 h or (c) 115 W m2 from fluorescentand tungsten lamps for 12 h extended to 16 h with low intensity(3 W m2) incandescent light from the tungsten lamps only.Plant growth was increased similarly in both long day treatments[(b) and (c)] and dry weights were 25 per cent greater thanin the 12 h photoperiod (a) after 6 weeks. Leaf area was increasedby 18 per cent and net assimilation rate by 10 per cent in the16 h photoperiod at 88 W m2 (b). By contrast, extendingthe photoperiod with 4 h of incandescent light (c) triggereda photomorphogenic increase in leaf expansion which increasedleaf area per plant by 47 per cent and leaf-area ratio by 12per cent. 相似文献
15.
An existing system of flowing solution culture, in which pHand the concentration of several nutrient ions are automaticallymonitored and controlled, has been extended to include at 10 mmol N m3. A brief account is givenof the use of the equipment with a simulated sward of perennialryegrass (Lolium perenne L.). Uptake of measured over three successive days in June, varied with dailysolar radiation and exceeded 1000 mg N m2 d1.The uptake of showed a pattern of diurnal variation similar to the variation in solar radiation, but witha lag period for uptake of 5 h. Hourly uptake rates ranged from32 to 67 mg N m2h1 and solar radiation from 0to 2.8 MJ m2 h1. During a 24 h period, additionalmeasurements were made of K+ uptake and net H+ efflux, bothof which showed patterns of diurnal variation with lag periodsof 6 h hand 7 h, respectively. The stoichiometric ratio of thesum of and K+ to the net efflux of H+ was1.02: 1. Key words: Ion uptake, diurnal variation, Lolium perenne L. 相似文献
16.
Knight, S. L. and Mitchell, C. A. 1988. Effects of CO2 and photosyntheticphoton flux on yield, gas exchange and growth rate of Lactucasativa L. Waldmanns Green'.J. exp. Bot.39: 317328. Enrichment of CO2 to 46 mmol m3 (1 000 mm3 dm3)at a moderate photosynthetic photon flux (PPF) of 450 µmolm2 s1 stimulated fresh and dry weight gain oflettuce leaves 39% to 75% relative to plants at 16 mmol m3CO2 (350 mm3 dm3). Relative growth rate (RGR) was stimulatedonly during the first several days of exponential growth. ElevatingCO2 above 46 mmol m3 at moderate PPF had no further benefit.However, high PPF of 880900 µmol m2 s1gave further, substantial increases in growth, RGR, net assimilationrate (NAR) and photosynthetic rate (Pn), but a decrease in leafarea ratio (LAR), at 46 or 69 mmol m3 (1000 or 1500 mm3dm3) CO2, the differences being greater at the higherCO2 level. Enrichment of CO2 to a supraoptimal level of 92 mmolm3 (2000 mm3 dm3) at high PPF increased leaf areaand LAR, decreased specific leaf weight, NAR and Pn and hadno effect on leaf, stem and root dry weight or RGR relativeto plants grown at 69 mmol m3 CO2 after 8 d of treatment.The results of the study indicate that leaf lettuce growth ismost responsive to a combination of high PPF and CO2 enrichmentto 69 mmol m3 for several days at the onset of exponentialgrowth, after which optimizing resources might be conserved. Key words: Photosynthesis, relative growth rate, CO2 enrichment 相似文献
17.
Density and distribution of bacterioplankton and planktonic ciliates in the Bering Sea and North Pacific 总被引:3,自引:0,他引:3
The total number of planktonic bacteria in the upper mixed layerof the Bering Sea during the late spring-early summer periodranged between 1 and {small tilde}4 x 106 ml1 (biomass1040mg C m3). In the northern Pacific, along 47526N,the corresponding characteristics of the bacterioplankton densityin the upper mixed water layer were: total number 12x 106 cells ml1 and biomass 1546mg C m3Below the thermocline at 50100 m, the density of bacterioplanktonrapidly decreased. At 300 m depth, it stabilized at 0.10.2x 106 cells ml1. The integrated biomass of bacterioplanktonin the open Bering Sea ranged between 1.2 and 3.6 g C m2(wet biomass 618 g m2) Its production per dayvaried from 2 to 23 mg C m3 days1 in the upper0100 m. The numerical abundance of planktonic ciliatesin this layer was estimated to be from 3 to l0 x 103 cells l1,and in the northern Pacific from 0.4 to 4.5 x 103 l2.Their populations were dominated by naked forms of Strombidium,Strombilidium and Tontonia. In some shelf areas, up to 40% ofthe total ciliate population was represented by the symbioticciliate Mesodinium rubrum. The data on the integrated biomassof basic groups of planktonic microheterotrophs are also presented,and their importance in the trophic relationships in pelagiccommunities of subarctic seas is discussed. 相似文献
18.
KEYS A. J.; SAMPAIO E. V. S. B.; CORNELIUS M. J.; BIRD I. F. 《Journal of experimental botany》1977,28(3):525-533
Wheat plants were grown in a controlled environment with daytemperatures of 18 ?C and with 500 µ Einsteins m281 of photosynthetically active radiation for 16 h. Beforeanthesis and 2 to 3 weeks after, rates of net photosynthesiswere measured for leaves in 2 or 21% O2 containing 350 vpm CO2at 13, 18, 23, and 28 ?C and with 500 µEinsteins m2s1 of photosynthetically active radiation. Also, underthe same conditions of light intensity and temperature, therates of efflux of CO2 into CO2-free air were measured and,for mature flag leaves 3 to 4 weeks after anthesis, gross andnet photosynthesis from air containing 320 vpm 14CO2 of specificactivity 39?7 nCi µmol1. When the O2 concentration was decreased from 21 to 2% (v/v)the rate of net photosynthesis increased by 32 per cent at thelowest temperature and 54 per cent at the highest temperature.Efflux of CO2 into CO2-free air ranged from 38 per cent of netphotosynthesis at 13 ?C to 86 per cent at 28 ?C. Gross photosynthesis,measured by the 14C assimilated during 40 s, was greater thannet photosynthesis by some 10 per cent at 13 ?C and 17 per centat 28 ?C. These data indicate that photorespiration was relativelygreater at higher temperatures. 相似文献
19.
Summer nutrient dynamics in the Middle Atlantic Bight: primary production and utilization of phytoplankton carbon 总被引:2,自引:0,他引:2
Falkowski P.G.; Vidal J.; Hopkins T.S.; Rowe G.T.; Whitledge T.E.; Harrison W.G. 《Journal of plankton research》1983,5(4):515-537
In late summer, production and utilization of carbon in thestratified water of the Middle Atlantic Bight appears to approachsteady-state conditions. In the euphotic zone there is a paniculateorganic carbon (POC) pool of {small tilde}6000 mg Cm2.Primary production adds {small tilde}350 mg C m2 d1,while zooplankton ingestion removes {small tilde}450 mg C m2d1, of which {small tilde}135 mg C m2 d1are returned to the POC pool as feces. Sinking of POC averages{small tilde}240 mg C m 2 d1. Thus, there is anet loss from the euphotic zone of {small tilde}200 mg C m2d1, which represents a removal rate from the POC poolof {small tilde}3%d1. However, sinking losses of phytoplanktoncarbon from the POC pool were small ({small tilde}12 mg C m2d1), which suggests that most of the primary productionenters the pelagic food chain. This is in sharp contrast tothe conditions in early spring, when the POC pool increasessubstantially, and the main loss is sinking rather than heterotrophicconsumption.
*This research was supported by the U.S. Department of Energyunder Contract No. DE-AC02-76CH000I6. 相似文献
20.
Proline and Polyamine Involvement in Chilling Tolerance of Maize Suspension Cultures 总被引:9,自引:0,他引:9
We have assessed the effect of various medium supplements inpromoting the ability of maize (Zea mays L.) inbred FR27rhmsuspension cultures to grow following a period of 4 °C chillingstress. Following a 4 week exposure to 4 °C in culture mediumwithout proline, no cell growth occurred upon subsequent incubationat 28°C for 2 weeks. This inhibition was reversed when 3to 48 mol m3 proline or 0.1 mol m3 putrescineor 0.01 mol m3 spermidine were present in the mediumduring the chilling stress. On the other hand, suspensions weremade more sensitive to 4°C by blocking polyamine biosynthesiswith 1.0 mol m3 methylglyoxal bis (guanylhydrazone) (MGBG)or a combination of 1.0 mol m3 difluoromethylornithine(DFMO) and 1.0 mol m3 difluoromethylarginine (DFMA).The addition of 10 mol m3 putrescine to the suspensioncontaining DFMO and DFMA prevented the increased chilling sensitivity.Electrolyte leakage studies conducted to assess membrane integrityafter 4 weeks at 4°C and a 2 week regrowth period showedthat cells treated with no polyamines (control), 0.01 mol m3spermidine, 1.0 mol m3 putrescine, or 1.0 mol m3MGBG lost 43, 32, 14, and 100% of the total electrolyte pool,respectively. These results suggest that proline and polyaminesare beneficial for inducing chilling tolerance in FR27rhm suspension. Key words: Proline, polyamine, chilling stress 相似文献