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1.
We examined the influence of aluminum and calcium (and certain other cations) on hormone transport in corn roots. When aluminum was applied unilaterally to the caps of 15 mm apical root sections the roots curved strongly away from the aluminum. When aluminum was applied unilaterally to the cap and 3H-indole-3-acetic acid was applied to the basal cut surface twice as much radioactivity (assumed to be IAA) accumulated on the concave side of the curved root as on the convex side. Auxin transport in the apical region of intact roots was preferentially basipetal, with a polarity (basipetal transport divided by acropetal transport) of 6.3. In decapped 5 mm apical root segments, auxin transport was acropetally polar (polarity = 0.63). Application of aluminum to the root cap strongly promoted acropetal transport of auxin reducing polarity from 6.3 to 2.1. Application of calcium to the root cap enhanced basipetal movement of auxin, increasing polarity from 6.3 to 7.6. Application of the calcium chelator, ethylene-glycol-bis-(β-aminoethylether)- N, N, N′, N′-tetraacetic acid, greatly decreased basipetal auxin movement, reducing polarity from 6.3 to 3.7. Transport of label after application of tritiated abscisic acid showed no polarity and was not affected by calcium or aluminum. The results indicate that the root cap is particularly important in maintaining basipetal polarity of auxin transport in primary roots of corn. The induction of root curvature by unilateral application of aluminum or calcium to root caps is likely to result from localized effects of these ions on auxin transport. The findings are discussed relative to the possible role of calcium redistribution in the gravitropic curvature of roots and the possibility of calmodulin involvement in the action of calcium and aluminum on auxin transport. 相似文献
2.
Transport and metabolism of IAA-1- 14C in Coleus blumei Benth. was studied by means of a combination of liquid scintillation counting, autoradiography and thin-layer chromatography. Transport of IAA in petiole segments of increasing age (No. 2-8) was strictly polar in a basipetal direction. No acropetal movement occurred in either young or old tissues. The greatest amount, expressed as a percentage of the radioactivity lost from the donor block, was found in basal receivers on petiole number 2. There was gradually less transport in older segments. The recovery as a percentage of the radioactivity not accounted for by donor and receiver blocks, measured by counting the radioactivity in an acetonitrile-extract of petiole segments, was low: 25 to 50%. In this acetonitrile-soluble fraction evidence for different radioactive compounds was found, depending on the age of the tissue. A possible relationship between the amounts of auxin transported in the tissue and its corresponding metabolism is discussed. 相似文献
3.
Several differences in the translocation pattern of radioactive kinetin in plant petioles were determined. Radioactivity from kinetin-8- 14C (Kn*) moved from donor agar blocks through petioles of bean and cocklebur but not of cotton. There was no difference in basipetal or acropetal movement of radioactivity from Kn* in cocklebnr petioles, but there was in bean petioles. In bean petioles this movement was preferentially basipetal, but it was influenced by the age of the petiole and by the presence of added indoleactic acid. The combination treatment accelerated the basipetal movement of radioactivity from Kn* in young bean petioles and not in old ones. All data is based on radioactivity translocated into receiver agar blocks which were assayed individually in a liquid scintillation spectrometer. The results show that plant species, direction of transport, age of tissue, and presence of IAA can all influence the translocation of Kn* in petioles. 相似文献
4.
Summary Movement of both [ 3H]GA 1 and [ 14C]GA 3 through root segments from P. coccineus seedlings was basipetally polarised. The basipetal/acropetal ratio of radioactivity from [ 3H]GA 1 in agar receiver blocks was 9.2 for apical, elongating segments, and 4.0 for more basal, non-elongating segments. Polarity of gibberellin transport was restricted to the stele, and absent from cortical tissues. Transport of [ 14C]IAA through root segments to agar receivers was preferentially acropetal, particularly so in the stele. Despite the existence of basipetal polarity of gibberellin transport in the root, [ 3H]GA 1 injected into cotyledons moved into and acropetally along the seedling root. 相似文献
5.
Young internodes of Coleus blumei Benth. have long been known for their sizable amount of acropetal indoleacetic acid movement. However, plants of the same clone, under improved growing conditions, now show almost absolute basipetal polarity of 14C-indoleacetic acid, as measured by liquid scintillation counting of 14C in the receiver cylinders of agar. The ratio of basipetal to acropetal movement is now as much as 85:1, instead of the 3:1 ratio found years ago under conditions providing slower growth. 相似文献
6.
14C-Indoleacetic acid was applied to coleoptiles of corn ( Zea mays) and oat ( Avena sativa). The coleoptiles were detached from the endosperms at 6-minute intervals after indoleacetic acid application, and the radioactivity was determined in successive 2-millimeter regions. The rate (per cent per minute) of basipetal transport of indoleacetic acid is periodic in various regions of the coleoptile, with a period of about 20 minutes. The possible relation of this cyclic phenomenon to other rhythmic processes of similar periodicities is discussed. A distinct acropetal transport (against the concentration gradient) from the subapical region to the apical 2-millimeter region of the coleoptile was detected. 相似文献
7.
Polar transport of kinetin-8- 14C occurred in segments of petioles, hypocotyls, and roots of radish ( Raphanus sativus L.). The polarity was basipetal in petioles and hypocotyls and acropetal in roots. In segments excised from seedlings with fully expanded cotyledons, indole-3-acetic acid was required for polarity to develop. In hypocotyl segments isolated at this stage, basipetal and acropetal movements were equal during the first 12 hours of auxin treatment after which time acropetal movement declined. Pretreatment with auxin eliminated this delay in the appearance of polarity. In hypocotyl segments excised from seedlings with expanding cotyledons, exogenous auxin was unnecessary for polarity. Potassium cyanide abolished polarity at both stages of growth by allowing increased acropetal movement. The rate of accumulation of kinetin in receiver blocks was greater than the in vivo increase in cytokinin content of developing radish roots. 相似文献
8.
The effect of a 180° displacement from the normal vertical orientation on longitudinal growth and on the acropetal and basipetal movement of 14C-IAA was investigated in Avena sativa L. and Zea mays L. coleoptile sections. Inversion inhibits growth in intact sections (apex not removed) and in decapitated sections supplied apically with donor blocks containing auxin. Under aerobic conditions, inversion inhibits basipetal auxin movement and promotes acropetal auxin movement, whereas under anaerobic conditions, it does not influence the movement of auxin in either direction. Inversion retards the basipetal movement of the peak of a 30-minute pulse of auxin in corn. The inversion-induced inhibition of basipetal auxin movement is not explained by an effect of gravity on production, uptake, destruction, exit from sections, retention in tissue, or purely physical movement of auxin. It is concluded that inversion (a) inhibits basipetal transport, the component of auxin movement that is metabolically dependent, and as a result (b) inhibits growth and (c) promotes acropetal auxin movement. 相似文献
9.
Summary The movement of 14C from indole-3-acetic acid (IAA) 14C has been examined in 5 mm root segments of dark-grown seedlings of Helianthus annuus and Brassica oleracea. Contaminants from distilled water, phosphate buffer and the razor-blade cutter increase the decarboxylation of IAA- 14C, and cutting of root segments results in an activation of IAA-destroying enzymes at the cut surfaces. When these sources of errors were eliminated the following was shown: a) Both in sunflower and cabbage there is a slight acropetal flux of 14C through the root segments into the agar receiver blocks. The amount of 14C found in the receiver blocks increases with the lenght of the transport period. b) When the root segments, after the transport period, are cut in two equal parts and these assayed separately, the amounts of 14C in the two parts indicate a greater acropetal than basipetal transport. c) The total radioactivity of the receiver blocks is in part due to IAA- 14C and in part to 14CO 2, the latter being a result of enzymatic destruction of auxin. d) Addition of ferulic acid, an inhibitor of IAA oxidases, to the receiver blocks markedly inhibits the decarboxylation of IAA- 14C and thus increases the amount transported. This effect is more pronounced after a 20 hr than after a 6 hr transport period. 相似文献
10.
A mathematical model simulating spatial pattern formation (positioning) of floral organs is proposed. Computer experiment with this model demonstrated the following sequence of spatial pattern formation in a typical cruciferous flower: medial sepals, carpels, lateral sepals, long stamens, petals, and short stamens. The positioning was acropetal for the perianth organs and basipetal for the stamens and carpels. Organ type specification and positioning proceed non-simultaneously in different floral parts and organ type specification goes ahead of organ spatial pattern formation. Computer simulation of flower development in several mutants demonstrated that the AG and AP2 genes determine both organ type specification and formation of the zones for future organ development. The function of the AG gene is to determine the basipetal patterning zones for the development of the reproductive organs, while the AP2 gene maintains proliferative activity of the meristem establishing the acropetal patterning zone for the development of the perianth organs. 相似文献
11.
Indoleacetic acid (IAA)-5- 3H (2 × 10 −9M) was applied to intact roots of Phaseolus coccineus seedlings, at the apex or 2 cm above the apex, at various pHs and in the presence of Cu 2+ and NaCl. The transport of label in the roots was then examined after 6 h by cutting the roots into 1 mm sections above and below the zone of treatment. Basipetal movement from 2 cm above the apex was unafected by pH, Cu 2+ or NaCl. Acropetal movement from the same area decreased with increasing pH from 5.4 to 8.0, probably due to an effect of pH on the entry of IAA into the cells. pH had no effect on sucrose transport. Cu 2+ also inhibited acropetal movement but NaCl had no effect. Basipetal movement of label from the apex was reduced by Cu 2+ and increasing pH, but not as much as with acropetal movement, and increased by the presence of NaCl. These facts are interpreted as showing 3 different systems of IAA movement in intact roots: basipetal from 2 cm up the root in some extracellular physical system; acropetal from 2 cm up the root, and basipetal from the apex, in a metabolically dependent intracellular system, but in different tissues of the root. It is proposed that endogenous IAA not only moves into the root from the stem but is also synthesized in the root apex, and moves basipetally for a short distance to the root growing zone in a separate system from the IAA descending from the stem. 相似文献
12.
3H-IAA transport in excised sections of carnation cuttings was studied by using two receiver systems for recovery of transported
radioactivity: agar blocks (A) and wells containing a buffer solution (B). When receivers were periodically renewed, transport
continued for up to 8 h and ceased before 24 h. If receivers were not renewed, IAA transport decreased drastically due to
immobilization in the base of the sections. TIBA was as effective as NPA in inhibiting the basipetal transport irrespective
of the application site (the basal or the apical side of sections). The polarity of IAA transport was determined by measuring
the polar ratio (basipetal/acropetal) and the inhibition caused by TIBA or NPA. The polar ratio varied with receiver, whereas
the inhibition by TIBA or NPA was similar. Distribution of immobilized radioactivity along the sections after a transport
period of 24 h showed that the application of TIBA to the apical side or NPA to the basal side of sections, increased the
radioactivity in zones further from the application site, which agrees with a basipetal and acropetal movement of TIBA and
NPA, respectively. The existence of a slow acropetal movement of the inhibitor was confirmed by using 3H-NPA. From the results obtained, a methodological approach is proposed to measure the variations in polar auxin transport.
This method was used to investigate whether the variations in rooting observed during the cold storage of cuttings might be
related to changes in polar auxin transport. As the storage period increased, a decrease in intensity and polarity of auxin
transport occurred, which was accompanied by a delay in the formation and growth of adventitious roots, confirming the involvement
of polar auxin transport in supplying the auxin for rooting.
Received April 19, 1999; accepted December 2, 1999 相似文献
13.
Auxin transport is required for important growth and developmental processes in plants, including gravity response and lateral root growth. Several lines of evidence suggest that reversible protein phosphorylation regulates auxin transport. Arabidopsis rcn1 mutant seedlings exhibit reduced protein phosphatase 2A activity and defects in differential cell elongation. Here we report that reduced phosphatase activity alters auxin transport and dependent physiological processes in the seedling root. Root basipetal transport was increased in rcn1 or phosphatase inhibitor-treated seedlings but showed normal sensitivity to the auxin transport inhibitor naphthylphthalamic acid (NPA). Phosphatase inhibition reduced root gravity response and delayed the establishment of differential auxin-induced gene expression across a gravity-stimulated root tip. An NPA treatment that reduced basipetal transport in rcn1 and cantharidin-treated wild-type plants also restored a normal gravity response and asymmetric auxin-induced gene expression, indicating that increased basipetal auxin transport impedes gravitropism. Increased auxin transport in rcn1 or phosphatase inhibitor-treated seedlings did not require the AGR1/EIR1/PIN2/WAV6 or AUX1 gene products. In contrast to basipetal transport, root acropetal transport was normal in phosphatase-inhibited seedlings in the absence of NPA, although it showed reduced NPA sensitivity. Lateral root growth also exhibited reduced NPA sensitivity in rcn1 seedlings, consistent with acropetal transport controlling lateral root growth. These results support the role of protein phosphorylation in regulating auxin transport and suggest that the acropetal and basipetal auxin transport streams are differentially regulated. 相似文献
14.
Summary Indoleacetic acid (IAA)-5- 3H (2×10 -9) was applied to intact roots of Phaseolus coccineus seedlings at the apex or 2 cm above the apex, and the movement of IAA- 3H and its metabolites traced by sectioning and chromatography. Basipetal movement of label occurred for 2 cm or less, declining exponentially, and the amount increased with time. Acropetal transport from above the apex showed quantitatively less movement of radioactivity. After a 6h treatment period a decline of label occurred in the first 0.5cm, below which there was a long distance movement of small amounts of label, mainly in IAA, towards the apex where the label concentrated by a factor of approximately 2. Short-distance basipetal movement consisted of about equal amounts of IAA and metabolites, and only metabolites were found in areas more basipetal than 2cm. Label from solutions of sucrose- 14C and 3H 2O followed the same general pattern of movement as label from IAA- 3H, except that acropetal movement of water showed a steady decrease in the amount of label as the distance from the area of application increased. The short distance basipetal transport of label with the breakdown of IAA- 3H indicates that the extent of basipetal movement was limited by catabolic processes. The acropetal pattern of IAA- 3H movement with the concentration of the transported material close to the apex, is possibly the result of transport in the phloem. 相似文献
15.
In an effort to investigate possible involvement of abscisic acid (ABA) in foliar abscission processes, its movement and endogenous levels were examined in cotyledons taken from cotton seedlings ( Gossypium hirsutum L.) subjected to varying degrees of water deficit, a condition which initiates leaf abscission. Using a pulse-labeling technique to avoid complications of uptake and exit from the tissue, ABA-1- 14C movement was observed in both basipetal and acropetal directions in cotyledonary petioles taken from well watered, stressed, and rewatered plants. The label distribution patterns obtained after 1 and 3 hours of transport under all situations of water supply were diffusive in nature and did not change when tested under anaerobic conditions. The transport capacity of the petioles ranged from 3.6 to 14.4% ABA-1- 14C transported per hour at estimated velocities of 0 to 2 millimeters per hour. Comparison of basipetal and acropetal movement indicated a lack of polarity under all conditions tested. These low transport capacities and slow velocities of movement, when compared to the active transport systems associated with auxin movement, as well as the lack of anaerobic effects and polarity, suggest that ABA movement in cotton cotyledonary petiole sections is facilitated by passive diffusion. Increases in free and bound ABA in the lamina with increased water stress did not correlate with patterns of cotyledonary abscission. Thus, no evidence was found to suggest that ABA is directly involved in stress-induced abscission processes. 相似文献
16.
A critical review of the few papers on IAA- 14C movement in roots revealed apparent contradictions, as well as flaws in experimental design that would be apt to cause artifacts. The movement of 14C from IAA- 14C was studied in sections of Lens and Phaseolus roots, using a system 20 or more times as sensitive as any previously used. To make sure that our results with roots could be compared validly with published work on petioles and stems, we used the same techniques as we had earlier used for shoot structures. The results with Lens were similar in many ways to those for shoots: net movement into receiver blocks was very strongly polar, followed a linear course for several hours, and showed a velocity of the same order of magnitude as in shoots (and, in fact, very close in absolute value to that found in Coleus stem cylinders). Also, as with shoots, all the radioactivity in receiver blocks ran to the R F of IAA. The time-course of loss of counts from donor blocks was similar to that found in shoots. The 2 most striking differences from shoots were 1) the very low percentage of added 14C that was moved into the receivers (about one-tenth of the values for bean petioles), and 2) the fact that the polar movement was acropetal in roots, rather than basipetal as in shoots. Results with Phaseolus roots were similar to those for Lens, although an additional complication with Phaseolus roots was the indication of a transitory stage of weak basipetal polarity in the first few hours after excising the section. This stage was followed in a few hours by a stronger acropetal polarity. 相似文献
17.
In stem sections of lentil seedlings, there is a typical polar movement of IAA labelled with 14C. The degree of polarity, expressed as the ratio of basipetal to acropetal transport, was (25°C) 7.6. A decrease (from 25° to 15°C) and an increase (from 25° to 30°C) of temperature cause a reduction of the IAA uptake by the sections and a decrease of both the basipetal and the acropetal translocation of IAA. Results suggest that the basipetal as well as the acropetal movement of auxin, are dependent of a metabolical component which is discussed. 相似文献
18.
α-Naphthaleneacetic acid-C 14, labeled in the carboxyl group, was applied in blocks of agar to the distal and to the proximal (either apical or basal) ends of explants of Coleus. The radioactivity in receiver blocks at the opposite ends was measured. Acropetal transport was slight, only 4% of the basipetal transport. Translocation of NAA-C14 was polar in basipetal direction. Only 1.4% of the radioactivity lost from donor blocks at the apical position reached the receiver blocks; the greatest part remained in the tissue and was immobilized there. All activity found in receiver blocks at the basal end appeared to be still in the form of NAA. There were no differences between petiole tissue and stem tissue, so far as the transport of NAA is concerned. 相似文献
19.
The polar, basipetal transport of indoleacetic acid helps to maintain polarity of auxin movement in coleoptiles of Avena sativa L. by opposing acropetal diffusion. This conclusion is supported by 3 different kinds of experiments. In all 3 experiments, sections took up 14C carboxyl-labeled indole-3-acetic acid anaerobically, and the distribution of auxin within all sections was similar at the end of uptake. [List: see text] 相似文献
20.
The spatial pattern of acropetal and basipetal cytoplasmic streaming velocities has been studied by laser-Doppler-velocimetry
(LDV) in the positively gravitropic (downward growing) rhizoids of Chara globularis Thuill. and for the first time in the negatively gravitropic (upward growing) protonemata. The LDV method proved to be precise
and yielded reproducible results even when tiny differences in velocities were measured. In the apical parts of the streaming
regions of both cell types, acropetal streaming was faster than basipetal streaming. Starting at the apical reversal point
of streaming, the velocity increased basipetally with the distance from that point and became fairly constant close to the
basal reversal point; subsequently, the velocity decreased slightly acropetally as the apical reversal point was again approached.
There was no change in velocity at the basal reversal point. However, at the apical reversal point there was an abrupt decrease
in velocity. The pattern of the ratio of acropetal to basipetal streaming velocity (VR) was a function of the relative distance
of the site of measurement from the apical reversal point rather than a function of the absolute distance. Upon inversion
of the rhizoids, the VR decreased on average by 3.8% (±0.4%), indicating that the effect of gravity on the streaming velocity
was merely physical and without a physiological amplification. Rhizoids that had developed on the slowly rotating horizontal
axis of a clinostat, and had never experienced a constant gravity vector, were similar to normally grown rhizoids with respect
to VR pattern. In protonemata, the VR pattern was not significantly different from that in rhizoids although the direction
of growth was inverse. In rhizoids, oryzalin caused the polar organization of the cell to disappear and nullified the differences
in streaming velocities, and cytochalasin D decreased the velocity of basipetal streaming slightly more than that of acropetal
streaming. Cyclopiazonic acid, known as an inhibitor of the Ca 2+-ATPase of the endoplasmic reticulum, also reduced the streaming velocities in rhizoids, but had slightly more effect on the
acropetal stream. It is possible that the endogenous difference in streaming velocities in both rhizoids and protonemata is
caused by differences in the cytoskeletal organization of the opposing streams and/or loading of inhibitors (like Ca 2+) from the apical/subapical zone into the basipetally streaming endoplasm.
Received: 4 October 1999 / Accepted: 4 November 1999 相似文献
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