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1.
An analysis was made of the size maturation process of nascent DNA intermediates in macronuclear DNA replication of Tetrahymena pyriformis. The first discrete size class of nascent intermediates larger than Okazaki fragments were replicon-sized DNA (about 2 X 10(7) D single-stranded (ss) DNA) and accumulated in cells treated with cycloheximide. On removal of cycloheximide, the replicon-sized intermediates were converted to middle-sized intermediates (about 10 X 10(7) D ssDNA) and then merged into chromosomal-sized DNA. As indicated by either aphidicolin inhibition or the technique of the photolysis of bromodeoxyuridine (BrdU)-substituted DNA with long-wave ultraviolet light, four to eight replicon-sized intermediates were joined together to form a middle-sized intermediate after rapid sealing by DNA synthesis of the late-replicating regions located between adjacent replicon-sized intermediates. The late-replicating regions may represent the short gaps or terminal regions where DNA synthesis was retarded by cycloheximide, since the size of late-replicating regions was suggested to be shorter than the replicon size by DNA fiber autoradiography. Therefore, it is probable that four to eight completed replicons are joined as a group such as a replicon cluster, as has been reported in DNA replication of other eukaryotic cells.  相似文献   

2.
The influence of 5-amino uracil (5-AU) was investigated on the cell cycle of log growth and division-synchronized Tetrahymena pyriformis GL. The division index of log growth phase Tetrahymena was suppressed by 50% after 40 min in 8 mM 5-AU. Cells division-synthronized by one heat shock per generation were also treated with 5-AU. Cells treated either prior to the first synchronous division (80 min EH) or up to 25 min prior to the second synchronous division (after 160 min EH) were not delayed in their progress through the cell cycle. Cells treated during the S phase of the first free running cell cycle, however, were delayed 5-30 min from reaching the second synchronous division. The effect of 5-AU on DNA and RNA synthesis was also examined. Incorporation of [3H]thymidine into acid-precipitable material was reduced in the presence of 5-AU; the rate of DNA synthesis was also reduced. The depression in the rate of DNA synthesis was greater at the beginning of S than at the end of S. The size of the thymidine pool (nucleosides + nucleotides) did not change during 5-AU treatment; however, an accumulation of thymidine tri-phosphate and a decrease in the amount of thymidine nucleoside was observed. A suppression of [14C]uridine incorporation resulting from 5-AU treatment was observed throughout the cell cycle. The rate of RNA synthesis as monitored by [14C]uridine incorporation into acid precipitable material was also reduced during 5-AU treatment. No change in either the size or the composition of the pool of uridine (nucleoside + nucleotide) was detected in 5-AU treated cells as compared to controls.  相似文献   

3.
Summary Isolabeling observed during sister chromatid differentiation (SCD) was studied from human skin fibroblasts by the fluorescence-plus-Giemsa (FPG) technique. Bromodeoxyuridine (BrdU) was fed to exponentially dividing cells for 52 h to enable completion of two consecutive cycles of DNA replication. During this period, the late-replicating regions of some chromosomes were able to go through three replication cycles. These chromosome regions had evidently incorporated BrdU bifiliarly in both chromatids and hence, on staining with FPG, appeared isostained (isolabeled). Thus, incubation of exponentially dividing cells with BrdU for a period longer than that required for two cell cycles appears to be a suitable method for revealing the late-replicating regions of the genome, such as the X chromosome in a human female, as isolated.In another experiment with Indian muntjac chromosomes, isolabeled segments were darkly stained, which suggested unifilar incorporation of BrdU. In this case, unequal crossing-over or an unequal distribution of thymine residues probably is responsible for the isolabel.  相似文献   

4.
Summary Barr body staining procedures were applied directly to the chamber slide cultures of female amniotic cells, WI38 fibroblasts, normal female kidney cells, and a human breast carcinoma cell line, Elco. A high frequency of Barr bodies was found in all the normal female control cells; however, no Barr bodies were observed in the Elco cells. By trypsin G-banding analysis, two normal X chromosomes were identified in all Elco cells. The late DNA replication pattern of the cell line was then studied with the terminal BrdU pulse method. Both X chromosomes in the Elco cell line were found to be euchromatic with a characteristic R-banding pattern; no late-replicating X chromosome was observed. The absence of both a Barr body and a late-replicating heterocyclic X chromosome provides strong cytogenetic evidence that an inactivated X chromosome is absent in the human breast carcinoma cells bearing two X chromosomes.  相似文献   

5.
Treatment of Vicia faba lateral roots with a range of concentrations of 5-aminouracil (5-AU) indicate that cells are stopped at a particular point in interphase. The timing of the fall in mitotic index suggests that cells are held at the S - G2 transition. When cells are held at this point, treatments with 5-AU can be used to estimate the duration of G2 + mitosis/2 of proliferating cells. Treatment with 5-AU can also be used to demonstrate the presence of subpopulations of dividing cells that differ in their G2 duration. Using this method, 5-AU-induced inhibition, we have confirmed that in V. faba lateral roots there are two populations of dividing cells: (a) a fast-dividing population, which makes up ~85% of the proliferating cell population and has a G2 + mitosis/2 duration of 3.3 hr, and (b) a slow-dividing population, which makes up ~15% of dividing cells and has a G2 duration in excess of 12 hr. These estimates are similar to those obtained from percentage labeled mitosis (PLM) curves after incorporation of thymidine-3H.  相似文献   

6.
Combining higher resolution chromosome analysis and bromodeoxyuridine (BrdU) incorporation, our study demonstrates that: (1) Human chromosomes synthesize DNA in a segmental but highly coordinated fashion. Each chromosome replicates according to its innate pattern of chromosome structure (banding). (2) R-positive bands are demonstrated as the initiation sites of DNA synthesis in all human chromosomes, including late-replicating chromosomes such as the LX and Y. (3) Replication is clearly biphasic in the sense that late-replicating elements, such as G-bands, the Yh, C-bands, and the entire LX, initiate replication after it has been completed in the autosomal R-bands (euchromatin) with minimal or no overlap. The chronological priority of R-band replication followed by G-bands is also retained in the facultative heterochromatin or late-replicating X chromosome (LX). Therefore, the inclusion of G-bands as a truly late-replicating chromatin type or G(Q)-heterochromatin is suggested. (4) Lateral asymmetry (LA) in the Y chromosome can be detected after less than half-cycle in 5-bromodeoxyuridine (BrdUrd), and the presence of at least two regions of LA in this chromosome is confirmed. (5) Finally, the replicational map of human chromosomes is presented, and a model of replication chronology is suggested. Based on this model, a system of nomenclature is proposed to place individual mitoses (or chromosomes) within S-phase, according to their pattern of replication banding. Potential applications of this methodology in clinical and theoretical cytogenetics are suggested.  相似文献   

7.
The DNA lesions responsible for the formation of sister chromatid exchanges (SCEs) have been the object of research for a long time. SCEs can be visualized by growing cells for either two rounds of replication in the presence of 5-bromo-2'-deoxyuridine (BrdU) or for one round with BrdU and the next without. If BrdU is added after cells were treated with a DNA-damaging agent, the effect on SCEs can only be analyzed in the second post-treatment mitosis. If one wishes to analyze the first post-treatment mitosis, cells unifilarily labeled with BrdU must be treated. Due to the highly reactive bromine atom, BrdU interacts with such agents like ionizing and UV radiation enhancing the frequency of SCEs. However, its precise role in this process was difficult to assess for a long time, because no alternative technique existed that allowed differential staining of chromatids. We have recently developed a method to differentially label sister chromatids with biotin-16-2'-deoxyuridine-5'-triphosphate (biotin-dUTP) circumventing the disadvantage of BrdU. This technique was applied to study the SCEs induced by ionizing and UV radiation as well as by mitomycin C, DNaseI and AluI. This article is a review of the results and conclusions of our previous studies.  相似文献   

8.
Developing lateral roots of V. faba were treated with 5-aminouracil for up to 6 hours using the 5-AU inhibition method discussed in this paper; the duration of G2+mitosis/2 and the percentages of slow dividing cells were estimated from the fall in MI observed in just emerged meristems, very large primordia and large primordia. The results indicate that during the period of development studied here there are two subpopulations of dividing cells: 1) fast dividing population which makes up about 84 % of the dividing cells and which has a G2+mitosis/2 duration of about 3.3 hours, and 2) a slow dividing population which constitutes about 16 % of the dividing cells and which has a G2 duration in excess of 12 hours. This heterogeneity is discussed in relationship to the behaviour of different populations of proliferating cells during root morphogenesis.  相似文献   

9.
These studies were undertaken to examine the relationship between the inhibition by 5-bromodeoxyuridine (BrdU) of erythroid differentiation in Friend erythroleukemia cells and the incorporation of BrdU into DNA. Experiments were carried out in which the incorporation of BrdU into DNA and the concentration of BrdU to which the cells were exposed were varied independently of each other. In addition, the ability of deoxycytidine (dC) to reverse the effects of BrdU on hemoglobin production and to reduce the amount of BrdU in DNA was analyzed. Under all the conditions tested, the effects of BrdU were correlated with the amount of BrdU incorporated into nuclear DNA. These results differ from those of recent studies on the inhibition of pigmentation and the induction of mutations by BrdU in Syrian hamster melanoma cells. The results suggest that BrdU may be producing its biological effects by a variety of different mechanisms.  相似文献   

10.
The purpose of this study was to investigate the cell cycle perturbation of cultured C6 rat glioma cells induced by 1-(4-amino-2-methyl-5-pyrimidyl)methyl-3-(2-chloroethyl)3-nitrosourea hydrochloride (ACNU) using simultaneous flow cytometric measurements of DNA and bromodeoxyuridine (BrdU) content. A new graphic computer program permitted the quantification of cell density in hexagonal subareas and allowed the fraction of BrdU-labeled cells with mid-S phase DNA content (FLS) to be defined in a narrow window. The cell kinetic parameters such as cell cycle time (Tc) and S phase time (Ts) were estimated from a manually plotted FLS curve at 18 and 6 hr, respectively. The major effect of ACNU on the cell cycle was an accumulation of the cells in the G2M phase 12 to 24 hr posttreatment when compared to G2M traverse of untreated cells. For the two-dimensional analysis, cells were labeled with BrdU and then treated with ACNU, or treated with ACNU and then labeled with BrdU. It was concluded that the cells in the S and G2M phases at the time of ACNU administration progressed to mitosis but that the G1 phase cells accumulated in the subsequent G2M phase. Two-dimensional FCM analysis using BrdU provided a useful tool in studying cell cycle perturbation.  相似文献   

11.
Summary Lateral roots ofVicia faba were treated with a solution of 5-aminouracil (3.93 × 10–3 M). They were either treated for 6 hours and allowed to recover for up to 10 hours, or were treated continuously for up to 24 hours. Mitotic index decreased as the duration of treatment increased,e.g., it was < 0.5 after 6 hours treatment and 4 hours recovery and 0.23 after 12 hours continuous treatment. During this period of low mitotic activity nuclei and cells increased in size: mean nuclear volume, for example, was 1505±651 m3 8 hours after the end of a 6 hours treatment. In roots treated continuously, nuclear volume increased from 559±204 m3 at 0 hour to 1272±636 m3 at 12 hours. In the first 3 hours it was the larger nuclei that grew,i.e., nuclei that would have proceeded into mitosis if they had not been blocked by 5-AU. But between 3 and 12 hours of continuous exposure to 5-AU all nuclei increased in volume. Cells, on the other hand, showed no response during the first 6 hours of treatment; their areas did not increase till 6–12 hours had elapsed. It appears that in cells blocked by 5-AU growth continues for about 12 hours. Initially, nuclei grow disproportionately large, suggesting that synthesis of nuclear components is favoured at the expense of cytoplasmic constituents, at least during the first 6 hours of treatment; there is an internal imbalance between nuclear and cell growth and a temporary change in the nuclear cytoplasmic ratio. When cells recover from the 5-AU block and enter mitosis their prophase nuclei are also much larger than those of untreated cells. The response to 5-AU is discussed in terms of internal restrictions on cell growth, due to the presence of cell walls, and the heterogeneity in nuclear volumes.  相似文献   

12.
Summary Utilizing the 5-bromo-deoxyuridine (BrdU) incorporation technique, we have recently studied the DNA replication kinetics in a dicentric X chromosome, formed by long arm-to-long arm fusion at band q23, from a 16-year-old black female with primary amenorrhea. The patient has a karyotype 45,X/46,X,dic(X)(q23).In the buccal smear the presence of X chromatin was found in 33% of the cells examined. The Barr bodies are large and 21% of them are bipartite. DNA replication studies were performed on the patient's lymphocytes by the thymidine pulse (T-pulse) method and confirmed comparatively by the BrdU pulse (B-pulse) method. The results indicate that the dicentric X chromosome is always late-replicating. The replication pattern is symmetric on both sides of the breakpoint and the replication sequence is, in order, p11, p22, q1(1–3), q22, q23, p21, and q21. This finding is comparable to those of other investigators and supports the theory that there exist two inactivation centers in the dicentric X chromosome, located on or near the q21 band.  相似文献   

13.
On the influence of 5-Amino uracil on the cell cycle of root tip meristems   总被引:1,自引:0,他引:1  
To study the influence of 5-AU on the cell cycle of meristematic root tip tissue the following quantities, among others, have been investigated during and after the 5-AU treatment: mitotic index, labeling index, rate of DNA synthesis, and alteration of the frequency of cells with nuclei of certain DNA content. The rate of DNA synthesis was determined by measuring the rate of 3H-TdR incorporation, after a 5-AU/TdR antagonism could be excluded. Following from these investigations, 5-AU reduces the rate of DNA synthesis; it does not block it completely, as FUdR does. The reduction of the rate of DNA synthesis prolongs the S phase, which results in an accumulation of cells in this state, in fact favourably in the last 1/3 of the S phase. In this connection the possibility of a favoured reduction of DNA synthesis in heterochromatic areas is discussed. Although 5-AU also influences G2, no accumulation of cells in this state has been observed. On the basis of the present results we have to deny the assumption made by several authors that 5-AU acts in the same way as FUdR.  相似文献   

14.
Poly-D-lysine has been reported to induce a triggering of mitosis in plant cells due to a selective stimulatory effect on cells arrested in G2. Root-tip cells of Allium cepa L. were first exposed to maleic hydrazide (MH) early in the cell cycle and posttreated with different concentrations of the polycationic agent while in G2. The result was a dose-dependent potentiation of chromosome damage observed at metaphase without any apparent effect induced by poly-D-lysine itself. The enhancement of the yield of chromosomal aberrations was concomitant with an increase in the frequency of mitosis. In order to test further the stimulatory effect of poly-D-lysine on mitosis, as well as the consequences of a shortening of the time available for repair, cells synchronized by protracted treatment with 5-aminouracil (5-AU), which also induces chromosome damage, were allowed to recover in the presence of the polycationic compound. Our data show that a premature arrival at mitosis resulted in an increase in the frequency of damaged cells observed.  相似文献   

15.
The immortal strand hypothesis proposes that asymmetrically dividing stem cells (SCs) selectively segregate chromosomes that bear the oldest DNA templates. We investigated cosegregation in neural stem cells (NSCs). After exposure to the thymidine analogue 5-bromo-2-deoxyuridine (BrdU), which labels newly synthesized DNA, a subset of neural precursor cells were shown to retain BrdU signal. It was confirmed that some BrdU-retaining cells divided actively, and that these cells exhibited some characteristics of SCs. This asymmetric partitioning of DNA then was demonstrated during mitosis, and these results were further supported by real time imaging of SC clones, in which older and newly synthesized DNA templates were distributed asymmetrically after DNA synthesis. We demonstrate that NSCs are unique among precursor cells in the uneven partitioning of genetic material during cell divisions.  相似文献   

16.
The mechanism of mitotic synchronization induced by 5-aminouracil (5-AU) in onion root meristems has been investigated. We studied the effect of the chemical upon asynchronous meristematic cells, as well as upon a synchronous fraction of them the position of which in the cell cycle was known. The kinetic studies have shown a fairly linear relationship between the treatment time and the induced mitotic wave. Therefore, cell accumulation in a particular zone of interphase appears to be the cause of mitotic synchronization. This zone seems to be located at the end of S period. 5-AU induces maximal mitotic delay on the cells located in the accumulation zone and severely depresses the S-G2 transit while the passage through G 2 remains relatively unaffected. The possibility that preferential inhibition affects the late replicating DNA synthesis is discussed.  相似文献   

17.
The sequential organisation of replication foci during S phase in onion ( Allium cepa) and their relationship to the nuclear matrix were investigated. To discern their structural features and temporal firing sequence, immunodetection of 5-bromo-2'-deoxyuridine (BrdU) was carried out after in vivo feeding in synchronised cells released from a 14-h-long hydroxyurea block. Replication foci consisted of small replication granules, called replisomes, which clustered together. Analysis of synchronous binucleate cells that maintained in their two nuclei the specular symmetry of distribution of sister chromosomes in anaphase, showed that replication starts in small replication foci at the telomeric pole (pattern I), though the telomeres themselves formed large foci that were late-replicating. The rDNA replication foci (pattern II) also become replicated in early S phase. Replication of large foci, including the heterochromatin (IV), occurred in late S phase and finished at the centromeric nuclear pole (pattern V). Labelling of proliferating cell nuclear antigen (PCNA) in nuclear matrices, prepared from S-phase nuclei after extensive DNase digestion, demonstrated that replication foci were always stably anchored to the nuclear matrix. Thus, association with the nucleoskeleton is not exclusively mediated by the replicating or nascent DNA. The overlapping of patterns I, II and III in the nuclear matrix, in contrast to the results of BrdU localisation in nuclei, suggests that PCNA becomes associated with the nuclear matrix before the replication foci are operative, and remains bound during replication.  相似文献   

18.
In Allium cepa meristematic cells, BrdU incorporation into late-replicating DNA and its detection by FPG technique has shown to allow the visualization of characteristic late replicated segments (LRS) in metaphase chromosomes not detected till now. Since these LRS have very similar characteristics with the 'dot-like' exchanges reported previously in this system, they seem to represent a new support for the idea of a regular organization of chromatin in metaphase.  相似文献   

19.
Although 5-bromodeoxyuridine (BrdU) does not interfere with the rate of proliferation, or with total protein, DNA, or RNA synthesis in normal mouse spleen B cells exposed to specific antigenic excitation or to polyclonal activators, this agent has the capacity to block the appearance of IgM antibody-forming cells (AFC) in response to antigenic challenge in vitro with sheep red blood cells (sRBC), as well as the generation of anti-sRBC or 2,4,6-trinitrobenzene sulfonate-AFC in the course of polyclonal expansion. The effect of BrdU can be blocked competitively by a 10-fold excess of thymidine (TdR). CsCl density equilibrium analysis indicates that B cells with 55% BrdU substitution of TdR in a single DNA strand could participate in at least one additional division cycle, resulting in a double DNA strand substitution. BrdU substitution in a single DNA strand was sufficient, however, to block antibody secretion in B cells (unifilar dominance). When cells with single-strand BrdU substitution were allowed to proliferate in the presence of a 10-fold excess of TdR, the cells regained the capacity to synthesize and secrete specific antibody, demonstrating the reversibility of the inhibitory effect of BrdU upon this specialized B-cell function. Cells stimulated with LPS in the presence of BrdU exhibited higher rates of synthesis of total RNA as well as of the RNA species of the translation machinery (28, 18, and 5S and tRNA), measured by the rate of incorporation of radioactive precursors at the time of peak proliferation. Exposure to BrdU blocked the secretion of μ and light chains, although such cells had high levels of membrane IgM (mIgM) and were able to regenerate mIgM after enzymatic stripping. The regeneration of mIgM could be suppressed with α-amanitine and by actinomycin D, suggesting that the incorporation of BrdU into DNA does not affect the transcripition of nucleotide sequences for mIgM. MOPC 315 and 70 plasmocytoma cells cultured in the presence of BrdU for 60 hr regularly incorporated BrdU into one and two DNA strands. There was a 55–60% substitution of BrdU in single DNA strands. This situation produces complete inhibition of the production of IgM, μ, or light chains in normal cells. In contrast, plasmocytoma cells treated with BrdU secreted as many α, γ, and light chains as the corresponding control cells grown in the presence of TdR. This result points to possibly profound differences in the regulation of transcripition of Ig nucleotide sequences in lymphocytes which have undergone malignant transformation.  相似文献   

20.
Abstract— Cells of an adrenergic clone M1 of the mouse neuroblastoma C 1300 show morphological differentiation when treated with BrdU (10−5 m ). The involvement in this phenomenon of an action of BrdU at the membrane level has been suggested (see References). Taking into account the alterations observed after addition of BrdU in culture cell lines it was interesting to investigate the incorporation of BrdU into DNA of neuroblastoma cells. Our results showed an active BrdU incorporation into the DNA of treated Ml cells (an average of 25% of all thymidine residues for the 10−5 m -BrdU concentration present in our experimental conditions). Thymidine substitution by BrdU did not occur randomly, indicating the existence of selective parts in the genome sensitive toward BrdU. In mitomycin–treated cells (at high doses) BrdU incorporation occurred significantly at a very low level when related to the total thymidines (2%). Our findings suggest that since DNA synthesis is not inhibited BrdU may act by being incorporated into DNA and thus altering gene expression in addition to a site of action on the cell membranes.  相似文献   

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