共查询到20条相似文献,搜索用时 15 毫秒
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Mapping of the SV40 specific sequences transcribed in vitro from chromatin of SV40 transformed cells. 总被引:4,自引:0,他引:4
S M Astrin 《Biochemistry》1975,14(12):2700-2704
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S Saragosti B Lescure M Yaniv 《Biochemical and biophysical research communications》1979,88(3):1077-1084
Pure wheat germ RNA polymerase II but not calf thymus RNA polymerase II forms relatively stable binary complexes (half life time of 30 minutes at 0°C) with superhelical SV 40 DNA. On the contrary, the addition of a specific dinucleotide and a single ribotriphosphate permits the formation of highly stable complexes between both enzymes and SV 40 DNA. The elongation of RNA chains with preinitiated wheat germ enzyme only is stimulated by sarkosyl. These observations suggest that the wheat germ enzyme, as compared to that isolated from calf thymus, may contain a protein factor, a more native structure or both that permit efficient initiation and elongation of RNA chains on double stranded DNA. 相似文献
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Preferential in vitro assembly of nucleosome cores on some AT-rich regions of SV40 DNA. 总被引:16,自引:7,他引:9 下载免费PDF全文
We have found that nucleosomes reconstituted from histone octamers and SV40 DNA Form I by progressively decreasing the salt concentration from 2 M NaCl are formed preferentially around 0.27, 0.37, 0.50 and 0.85 on SV40 DNA (relative to the EcoRI site). When SV40 DNA Form III is used, the nucleosomes form mainly at 0.28, 0.38, 0.61 and 0.83. These sites are very close to both the sites of RNA chain initiation by calf thymus RNA polymerase B on SV40 DNA Form I (0.25, 0.35, 0.42 and 0.88) and the regions of the supercoiled DNA which are readily denaturable by T4 gene 32 protein (0.25, 0.47 and 0.88), and correspond to AT-rich regions as deduced from the nucleotide sequence of SV40 DNA. The physiologically important region around 0.67 is an unfavourable site for all three types of proteins, and corresponds to a GC-rich region surrounding a 17 base pair AT cluster. 相似文献
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Preferred Site for Initiation of RNA Transcription by Escherichia coli RNA Polymerase Within the Simian Virus 40 DNA Segment of the Nondefective Adenovirus-Simian Virus 40 Hybrid Viruses Ad2+ND1 and Ad2+ND3 总被引:12,自引:6,他引:6 下载免费PDF全文
B. Sayeeda Zain Ravi Dhar Sherman M. Weissman Paul Lebowitz Andrew M. Lewis Jr. 《Journal of virology》1973,11(5):682-693
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A mammalian DNA polymerase alpha holoenzyme functioning on defined in vivo-like templates. 总被引:7,自引:1,他引:6 下载免费PDF全文
In analogy to the Escherichia coli replicative DNA polymerase III we define two forms of DNA polymerase alpha: the core enzyme and the holoenzyme. The core enzyme is not able to elongate efficiently primed single-stranded DNA templates, in contrast to the holoenzyme which functions well on in vivo-like template. Using these criteria, we have identified and partially purified DNA polymerase alpha holoenzyme from calf thymus and have compared it to the corresponding homogeneous DNA polymerase alpha (defined as the core enzyme) from the same tissue. The holoenzyme is able to use single-stranded parvoviral DNA and M13 DNA with a single RNA primer as template. The core enzyme, on the other hand, although active on DNAs treated with deoxyribonuclease to create random gaps, is unable to act on these two long, single-stranded DNAs. E. coli DNA polymerase III holoenzyme also copies the two in vivo-like templates, while the core enzyme is virtually inactive. The homologous single-stranded DNA-binding proteins from calf thymus and from E. coli stimulate the respective holoenzymes and inhibit the core enzymes. These results suggest a cooperation between a DNA polymerase holoenzyme and its homologous single-stranded DNA-binding protein. The prokaryotic and the mammalian holoenzyme behave similarly in several chromatographic systems. 相似文献
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Transcription of Simian virus 40 DNA in a HeLa whole cell extract 总被引:22,自引:0,他引:22
H Handa R J Kaufman J Manley M Gefter P A Sharp 《The Journal of biological chemistry》1981,256(1):478-482
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