共查询到20条相似文献,搜索用时 500 毫秒
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Uddin S Lekmine F Sharma N Majchrzak B Mayer I Young PR Bokoch GM Fish EN Platanias LC 《The Journal of biological chemistry》2000,275(36):27634-27640
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The Streptococcus pyogenes orphan protein tyrosine phosphatase,SP‐PTP,possesses dual specificity and essential virulence regulatory functions
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Sashi Kant Shivani Agarwal Preeti Pancholi Vijay Pancholi 《Molecular microbiology》2015,97(3):515-540
Group A Streptococcus (GAS) is a human pathogen that causes high morbidity and mortality. GAS lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP‐PTP). Thus, GAS is thought to lack tyrosine phosphorylation, and the physiological significance of SP‐PTP is, therefore, questionable. Here, we demonstrate that SP‐PTP possesses dual phosphatase specificity for Tyr‐ and Ser/Thr‐phosphorylated GAS proteins, such as Ser/Thr kinase (SP‐STK) and the SP‐STK‐phosphorylated CovR and WalR proteins. Phenotypic analysis of GAS mutants lacking SP‐PTP revealed that the phosphatase activity per se positively regulates growth, cell division and the ability to adhere to and invade host cells. Furthermore, A549 human lung cells infected with GAS mutants lacking SP‐PTP displayed increased Ser‐/Thr‐/Tyr‐phosphorylation. SP‐PTP also differentially regulates the expression of ~50% of the total GAS genes, including several virulence genes potentially through the two‐component regulators, CovR, WalR and PTS/HPr regulation of Mga. Although these mutants exhibit attenuated virulence, a GAS mutant overexpressing SP‐PTP is hypervirulent. Our study provides the first definitive evidence for the presence and importance of Tyr‐phosphorylation in GAS and the relevance of SP‐PTP as an important therapeutic target. 相似文献
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Srivastava KK Batra S Sassano A Li Y Majchrzak B Kiyokawa H Altman A Fish EN Platanias LC 《The Journal of biological chemistry》2004,279(29):29911-29920
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Requirement for Ras/Rac1-mediated p38 and c-Jun N-terminal kinase signaling in Stat3 transcriptional activity induced by the Src oncoprotein. 总被引:9,自引:0,他引:9
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James Turkson Tammy Bowman Jalila Adnane Yi Zhang Julie Y. Djeu Madhavi Sekharam David A. Frank Lawrence B. Holzman Jie Wu Said Sebti Richard Jove 《Molecular and cellular biology》1999,19(11):7519-7528