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1.
The classic compartment analysis of ion efflux from roots is often applied with the assumption that there is a system of 3 compartments in series. However, complex ion transport across the root tissues, as well as influences from the shoot, may complicate the picture. The present experiments were performed to study the immediate effects that excision of the shoot before the experiment exerts on the efflux of Rb+(86Rb+) and of K+(86Rb+) from 9-day-old roots of plants of barley (Hordeum vulgare L. cv. Salve). The efflux from high K+ and low K+ roots of intact and detopped plants were compared. After excision of the shoot of high K+ plants, a marked increase in efflux was observed after 2.5 h with a maximum at about 7 h. The increase in efflux was seen as a peak in plots of efflux versus time. Excision of the shoot from low K+ roots did not give rise to a consistent increase in efflux. Regular K+ ion efflux curves were observed from roots of intact plants of high or low K+ status. Furthermore, after a pulse treatment of 9-day-old roots of intact plants of high or low K+ status with a solution containing Rb+(86Rb+), the Rb+(86Rb+) transport to the shoots was not reduced during the following 3 h in unlabelled solution. It is suggested that both the peak appearing in the efflux plots and the maintained tracer transport to the shoots after transfer of the roots to an unlabelled solution indicate the existence of a K+/Rb+ transport system in the symplasm of the roots that has only a slow exchange with the bulk cytoplasm and vacuoles. 相似文献
2.
Transport of Rb+ from the roots to the pulvinus and its diurnal movement in the pulvinus were investigated. Rb+ was given to the roots as its chloride or nitrate. The results showed that (1) Rb+ was transported from the roots to the pulvinus through the hypocotyl, epicotyl and petiole, (2) Rb+ was absorbed into the pulvinus in exchange for K+, (3) the absorbed Rb+ moved diurnally in the same phase as the remaining K+. Namely, Rb+ moved in the pulvinus diurnally just like K+. 相似文献
3.
Frozen aqueous suspensions of partially purified membrane-bound renal (Na+ + K+)-ATPase have been irradiated at –135°C with high-energy electrons. (Na+ + K+)-ATPase and K+-phosphatase activities are inactivated exponentially with apparent target sizes of , respectively. These values are significantly lower then found previously from irradiation of lyophilized membranes. After reconstitution of irradiated (Na+ + K+)-ATPase into phospholipid vesicles the following transport functions have been measured and target sizes calculated from the exponential inactivation curves: ATP-dependent Na+?K+ exchange, ; (ATP + Pi)-activated Rb+?Rb+ exchange, and ATP-independent Rb+?Rb+ exchange, . The apparent size of the α-chain, judged by disappearance of Coomassie stain on SDS-gels, lies between 115 and 141 kDa. That for the β-glycoprotein, though clearly smaller, could not be estimated. We draw the following conclusions: (1) The simplest interpretation of the results is that the minimal functional unit for (Na+ + K+)-ATPase is αβ. (2) The inactivation target size for (Na+ + K+)-dependent ATP hydrolysis is the same as for ATP-dependent pumping of Na+ and K+. (3) The target sizes, for K+-phosphatase (125 kDa) and ATP-independent Rb+?Rb+ exchange (117 kDa) are indistinguishable from that of the α-chain itself, suggesting that cation binding sites and transport pathways, and the phosphate binding site are located exclusively on the α-chain. (4) ATP-dependent activities appear to depend on the integrity of an αβ complex. 相似文献
4.
Emilia Ballesteros Eduardo Blumwald Juan Pedro Donaire rés Belver 《Physiologia plantarum》1997,99(2):328-334
Na+ transport across the tonoplast and its accumulation in the vacuoles is of crucial importance for plant adaptation to salinity. Mild and severe salt stress increased both ATP- and PPi-dependent H+ transport in tonoplast vesicles from sunflower seedling roots, suggesting the possibility that a Na+/H+ antiport system could be operating in such vesicles under salt conditions (E. Ballesteros et al. 1996. Physiol. Plant. 97: 259–268). During a mild salt stress, Na+ was mainly accumulated in the roots. Under a more severe salt treatment, Na+ was equally distributed in shoots and roots. In contrast to what was observed with Na+, all the salt treatments reduced the shoot K+ content. Dissipation by Na+ of the H+ gradient generated by the tonoplast H+-ATPase, monitored as fluorescence quenching of acridine orange, was used to measure Na+/H+ exchange across tonoplast-enriched vesicles isolated by sucrose gradient centrifugation from sunflower (Helianthus annuus L.) roots treated for 3 days with different NaCl regimes. Salt treatments induced a Na+/H+ exchange activity, which displayed saturation kinetics for Na+ added to the assay medium. This activity was partially inhibited by 125 μM amiloride, a competitive inhibitor of Na+/H+ antiports. No Na+/H+ exchange was detected in vesicles from control roots. The activity was specific for Na+. since K+ added to the assay medium slightly dissipated H+ gradients and displayed non-saturating kinetics for all salt treatments. Apparent Km for Na+/H+ exchange in tonoplast vesicles from 150 mM NaCl-treated roots was lower than that of 75 mM NaCl-treated roots, Vmax remaining unchanged. The results suggest that the existence of a specific Na+/H+ exchange activity in tonoplast-enriched vesicle fractions, induced by salt stress, could represent an adaptative response in sunflower plants, moderately tolerant to salinity. 相似文献
5.
《Plant Science Letters》1984,33(1):103-114
The effects of monovalent cations, inhibitors of metabolism dinitrophenol (DNP), carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP), and KCN and temperature variations upon Ca2+ fluxes in intact roots of barley (Hordeum vulgare L. cv. Fergus and Herta) seedlings were investigated. 45Ca2+ influx was depressed in CaSO4-grown (low-salt) plants by the presence of NH4+, K+, or Na+ in the uptake medium. In contrast Ca2+ influx was slightly increased by Li+. In low-salt roots pretreated with KCN and in roots preloaded with K+ (high-K+ plants), the presence of K+ in the medium had no significant effect on Ca2+ influx, while in roots preloaded with Na+, the presence of K+ in the medium depressed Ca2+ influx. In absolute terms, Ca2+ influx was significantly greater in high-salt (both K+ or Na+ preloaded) than in low-salt roots.Patterns of 45Ca2+ efflux in the absence and in the presence of K+, NH4+, or Li+ in the external medium showed that these monovalent cations caused stimulation of 45Ca2+ efflux both from the cytoplasmic and vacuolar phases.It was noted that these modifications of Ca2+ fluxes by monovalent cations are transient and characteristic of a transitional stage of cation uptake by low-salt roots. We conclude that, together with stimulated active H+ efflux (another characteristic of this transitional stage), modifications of Ca2+ fluxes during monovalent cation uptake by low-salt roots is a response directed towards the maintenance of electrical neutrality.Determination of net fluxes revealed that the plants were close to Ca2+ flux equilibrium in the growth medium (0.5 mM CaSO4). Transfer of these plants to 0.5 mM CaSO4 + 0.25 mM K2SO4 caused a net release of CA2+ into the external medium. 相似文献
6.
W. D. Jeschke 《Plant and Soil》1983,72(2-3):197-212
Summary In this short survey differences between species and varieties in the four major mechanisms that affect selective uptake of potassium and sodium to the plant within the root are considered. These include influx selectivity, K+/Na+ exchange at the plasmalemma, and selectivity at the tonoplast as well as at the symplasm-xylem boundary. The affinity of various plants for potassium influx in system 1 is rather uniform although varietal differences in barley have been observed. Differences are much more pronounced for sodium influx, for which Helianthus showed rather high and Fagopyrum rather low affinity. There is substantial variation between species in the efficiency of K+/Na+ exchange at the plasmalemma of cortical root cells; the three cereals Hordeum, Triticum, and Secale were highly efficient while K+/Na+ exchange in Atriplex, Helianthus and Allium was poor, even if the cytoplasmic sodium content was accounted for. Apparently there was no direct relation between salt tolerance and K+/Na+ exchange. The observed differences in the efficiency of K+-dependent sodium extrusion or K+/Na+ exchange were not due to the use of excised roots, they were observed also when roots of whole seedlings were investigated. At the tonoplast a 11 exchange of vacuolar potassium for sodium has been observed in roots of Hordeum. By this exchange sodium ions are removed from the symplasm and potassium ions are recovered from vacuoles and thus made available for transport to the shoot. Indications for specific differences in this exchange have been observed; the exchange appears to be more efficient in Helianthus than in Hordeum roots. More comparative studies are needed here. At the boundary between symplasm and xylem vessels selectivity can be set up during xylem release of cations and there are reports that suggest a preference for sodium (Lycopersicum cheesemanii, Solanum pennellii, and Suaeda) and for varietal differences amongst tomatoes. Selectivity at this boundary, the plasmalemma of the xylem parenchyma cells was described in this paper by the selectivity ratio of transport that relates the rates of xylem transport to the cytoplasmic sodium and potassium concentrations. Based on this ratioAtriplex hortensis was shown to discriminate for sodium during xylem release while there was little selectivity in Hordeum and possibly some discrimination in favour of K+ in Allium roots. The data are shortly discussed in relation to salt tolerance and to the breeding of salt-tolerant crop varieties. 相似文献
7.
86Rb+ uptake by yeast was not only stimulated by Rb+ or K+ but also by Na+. The uptake of 22Na+ was enhanced by both Rb+ and K+, but not by Na+, which was inhibitory at all concentrations applied. Inhibition of 22Na+ uptake by inactive Na+ occurred in two phases: one phase refers to inhibition at low Na+ concentrations and the other to inhibition at high Na+ concentrations. Our results can be qualitatively described by a two-site transport mechanism, having two cation binding sites, which must be occupied with monovalent cations before transport can occur. 相似文献
8.
The fluxes of 22Na+ and 86Rb+ in Arbacia sperm and oocytes were studied in order to determine how these cells carry out cation exchange with the sea environment. The uptake of these ions by serum followed a pattern of early rapid influx (initial 0.5 min) and subsequent efflux (1–3 min) followed by a gradual uptake (after 3 min). Neither the uptake nor the efflux of these cations by Arbacia sperm were affected by ouabain, suggesting that influx and efflux of 22Na+ and 86Rb+ in Arbacia sperm occur predominantly by passive transport. The 22Na+ uptake by Arbacia oocytes showed a steady increase after an initial rapid uptake. A slight but significant inhibition of 22Na+ uptake was observed with ouabain. However, 86Rb+ uptake by the oocytes reached an early equilibrium and was not affected by ouabain. The uptake of Rb+ by Arbacia oocyte is by passive transport while that of Na+ is both by passive and active transport. 相似文献
9.
This work presents a detailed kinetic study that shows the coupling between the E2→E1 transition and Rb+ deocclusion stimulated by Na+ in pig-kidney purified Na,K-ATPase. Using rapid mixing techniques, we measured in parallel experiments the decrease in concentration of occluded Rb+ and the increase in eosin fluorescence (the formation of E1) as a function of time. The E2→E1 transition and Rb+ deocclusion are described by the sum of two exponential functions with equal amplitudes, whose rate coefficients decreased with increasing [Rb+]. The rate coefficient values of the E2→E1 transition were very similar to those of Rb+-deocclusion, indicating that both processes are simultaneous. Our results suggest that, when ATP is absent, the mechanism of Na+-stimulated Rb+ deocclusion would require the release of at least one Rb+ ion through the extracellular access prior to the E2→E1 transition. Using vanadate to stabilize E2, we measured occluded Rb+ in equilibrium conditions. Results show that, while Mg2 + decreases the affinity for Rb+, addition of vanadate offsets this effect, increasing the affinity for Rb+. In transient experiments, we investigated the exchange of Rb+ between the E2-vanadate complex and the medium. Results show that, in the absence of ATP, vanadate prevents the E2→E1 transition caused by Na+ without significantly affecting the rate of Rb+ deocclusion. On the other hand, we found the first evidence of a very low rate of Rb+ occlusion in the enzyme–vanadate complex, suggesting that this complex would require a change to an open conformation in order to bind and occlude Rb+. 相似文献
10.
Steady state kinetics were used to examine the influence of Cd2+ both on K+ stimulation of a membrane-bound ATPase from sugar beet roots (Beta vulgaris L. cv. Monohill) and on K+(86Rb+) uptake in intact or excised beet roots. The in vitro effect of Cd2+ was studied both on a 12000–25000 g root fraction of the (Na++K++Mg2+)ATPase and on the ATPase when further purified by an aqueous polymer two-phase system. The observed data can be summarized as follows: 1) Cd2+ at high concentrations (>100 μM) inhibits the MgATPase activity in a competitive way, probably by forming a complex with ATP. 2) Cd2+ at concentrations <100 μM inhibits the specific K+ activation at both high and low affinity sites for K+. The inhibition pattern appears to be the same in the two ATPase preparations of different purity. In the presence of the substrate MgATP, and at K+ <5 mM, the inhibition by Cd2+ with respect to K+ is uncompetitive. In the presence of MgATP and K+ >10 μM, the inhibition by Cd2+ is competitive. 3) At the low concentrations of K+, Cd2+ also inhibits the 2,4-dinitrophenol(DNP)-sensitive (metabolic) K+(86Rb+) uptake uncompetitively both in excised roots and in roots of intact plants. 4) The DNP-insensitive (non metabolic) K+(86Rb+) uptake is little influenced by Cd2+. As Cd2+ inhibits the metabolic uptake of K+(86Rb+) and the K+ activation of the ATPase in the same way at low concentrations of K+, the same binding site is probably involved. Therefore, under field conditions, when the concentration of K+ is low, the presence of Cd2+ could be disadvantageous. 相似文献
11.
Summary To study the physiological role of the bidirectionally operating, furosemide-sensitive Na+/K+ transport system of human erythrocytes, the effect of furosemide on red cell cation and hemoglobin content was determined in cells incubated for 24 hr with ouabain in 145mm NaCl media containing 0 to 10mm K+ or Rb+. In pure Na+ media, furosemide accelerated cell Na+ gain and retarded cellular K+ loss. External K+ (5mm) had an effect similar to furosemide and markedly reduced the action of the drug on cellular cation content. External Rb+ accelerated the Na+ gain like K+, but did not affect the K+ retention induced by furosemide. The data are interpreted to indicate that the furosemide-sensitive Na+/K+ transport system of human erythrocytes mediates an equimolar extrusion of Na+ and K+ in Na+ media (Na+/K+ cotransport), a 1:1 K+/K+ (K+/Rb+) and Na+/Na+ exchange progressively appearing upon increasing external K+ (Rb+) concentrations to 5mm. The effect of furosemide (or external K+/Rb+) on cation contents was associated with a prevention of the cell shrinkage seen in pure Na+ media, or with a cell swelling, indicating that the furosemide-sensitive Na+/K+ transport system is involved in the control of cell volume of human erythrocytes. The action of furosemide on cellular volume and cation content tended to disappear at 5mm external K+ or Rb+. Thein vivo red cell K+ content was negatively correlated to the rate of furosemide-sensitive K+ (Rb+) uptake, and a positive correlation was seen between mean cellular hemoglobin content and furosemide-sensitive transport activity. The transport system possibly functions as a K+ and waterextruding mechanism under physiological conditiosin vivo. The red cell Na+ content showed no correlation to the activity of the furosemide-sensitive transport system. 相似文献
12.
PAUL JENSÉN 《Physiologia plantarum》1980,49(3):291-295
The influx of Rb+ into the roots of two barley varieties (Hordeum vulgare L. cv. Salve and cv. Ingrid) from a K+-free 86Rb-labelled nutrient solution with 2.0 mM Rb+, was checked at intervals from day 6 to day 18. The control plants were continuously grown in complete nutrient solution containing 5.0 mM K+, while two other groups of plants were grown in K+-free nutrient solution starting on day 6 and between day 6 and day 9, respectively. The pattern of Rb+ influx was similar for both varieties, although their efficiencies in absorbing Rb+ were different. The relationship between Rb+ influx and K+ concentration of the root could be interpreted in terms of negative feedback through allosteric control of uptake across the plasmalemma of the root cells. Hill plots were bimodal, but in the opposite direction. The Hill coefficients, reflecting the minimum number of interacting allosteric binding sites for K+ (Rb+), were low (≤–3.0). It is discussed whether the threshold value, that is the breaking point in the Hill plot, is indicative of a changed efficiency of transporting units for K+ (Rb+) transport to the xylem. Moreover, feedback regulation might be involved in transport of K+ between root and shoot. The variation in K+ concentrations in the roots and shoots of control plants were cyclic but in phase opposition despite an exponential growth. The average K+ concentration varied only slightly with age. 相似文献
13.
J. Pácha 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1993,163(6):495-498
To evaluate the developmental changes in colonic Na+ transport, Na, K-ATPase activity and the sensitivity of the short-circuit current to amiloride were investigated. The amiloride-sensitive short-circuit current which represents the electrogenic, amiloride-sensitive Na+ transport through Na+ channels, was not present in chicken embryos but rose significantly after hatching in chicks which were kept on a low-salt diet. Amiloride-sensitive short-circuit current increased gradually but the plateau was not reached during the first 15 days of life. Drinking of 0.9% NaCl totally inhibited the induction of amiloride-sensitive Na+ transport. Na+, K+-ATPase activity increased during development but was not influenced by changes in salt intake. Na+ transport in chicken colon therefore undergoes profound developmental changes. The increase of Na+ transport refleets not only the adaptation of colonocytes to low salt intake but also the maturation of Na+ absorption in colon. The possible role of aldosterone in the adaptation to low-salt intake is discussed.Abbreviations LS
low-salt
- HS
high-salt
-
I
sc
short-circuit current 相似文献
14.
Jochen Duhm 《The Journal of membrane biology》1987,98(1):15-32
Summary The effect of extracellular and intracellular Na+ (Na
o
+
, Na
i
+
) on ouabain-resistant, furosemide-sensitive (FS) Rb+ transport was studied in human erythrocytes under varying experimental conditions. The results obtained are consistent with the view that a (1 Na++1 K++2 Cl–) cotransport system operates in two different modes: modei) promoting bidirectional 11 (Na+–K+) cotransport, and modeii) a Na
o
+
-independent 11 K
o
+
/K
i
+
exchange requiring Na
i
+
which, however, is not extruded. The activities of the two modes of operation vary strictly in parallel to each other among erythrocytes of different donors and in cell fractions of individual donors separated according to density. Rb+ uptake through Rb
o
+
/K
i
+
exchange contributes about 25% to total Rb+ uptake in 145mm NaCl media containing 5mm RbCl at normal Na
i
+
(pH 7.4). Na+–K+ cotransport into the cells occurs largely additive to K+/K+ exchange. Inward Na+–Rb+ cotransport exhibits a substrate inhibition at high Rb
o
+
. With increasing pH, the maximum rate of cotransport is accelerated at the expense of K+/K+ exchange (apparent pK close to pH 7.4). The apparentK
m
Rb
o
+
of Na+–K+ cotransport is low (2mm) and almost independent of pH, and high for K+/K+ exchange (10 to 15mm), the affinity increasing with pH. The two modes are discussed in terms of a partial reaction scheme of (1 Na++1 K++2 Cl–) cotransport with ordered binding and debinding, exhibiting a glide symmetry (first on outside = first off inside) as proposed by McManus for duck erythrocytes (McManus, T.J., 1987,Fed. Proc., in press). N-ethylmaleimide (NEM) chemically induces a Cl–-dependent K+ transport pathway that is independent of both Na
o
+
and Na
i
+
. This pathway differs in many properties from the basal, Na
o
+
-independent K+/K+ exchange active in untreated human erythrocytes at normal cell volume. Cell swelling accelerates a Na
o
+
-independent FS K+ transport pathway which most probably is not identical to basal K+/K+ exchange. K
o
+
o
+
o
+
o
2+
reduce furosemide-resistant Rb+ inward leakage relative to choline
o
+
. 相似文献
15.
Summary Measurements are described of fusicoccin (FC)-stimulated H+ efflux in barley (Hordeum vulgare L.) roots when K+ and Na+ concentrations were varied. In low-salt roots H+ efflux was stimulated in both 5 mM KCl and NaCl. In salt-saturated roots H+ efflux was stimulated more effectively in KCl than in NaCl solution. The stimulation of H+ efflux thus is parallel with the selectivity of these different root preparations for K+ and Na+ and with estimates of permeability ratios (P
Na/P
K) determined from electrical measurements. It is suggested that the results support electrogenic coupling between FC-stimulated H+ efflux and cation uptake. 相似文献
16.
Jingyu Sun Junjun Xie Mengliang Niu Zhixiong Liu Molin Fan Zhilong Bie 《Physiologia plantarum》2014,152(4):738-748
Grafting onto salt‐tolerant pumpkin rootstock can increase cucumber salt tolerance. Previous studies have suggested that this can be attributed to pumpkin roots with higher capacity to limit the transport of Na+ to the shoot than cucumber roots. However, the mechanism remains unclear. This study investigated the transport of Na+ in salt‐tolerant pumpkin and salt‐sensitive cucumber plants under high (200 mM) or moderate (90 mM) NaCl stress. Scanning ion‐selective electrode technique showed that pumpkin roots exhibited a higher capacity to extrude Na+, and a correspondingly increased H+ influx under 200 or 90 mM NaCl stress. The 200 mM NaCl induced Na+/H+ exchange in the root was inhibited by amiloride (a Na+/H+ antiporter inhibitor) or vanadate [a plasma membrane (PM) H+‐ATPase inhibitor], indicating that Na+ exclusion in salt stressed pumpkin and cucumber roots was the result of an active Na+/H+ antiporter across the PM, and the Na+/H+ antiporter system in salt stressed pumpkin roots was sufficient to exclude Na+. X‐ray microanalysis showed higher Na+ in the cortex, but lower Na+ in the stele of pumpkin roots than that in cucumber roots under 90 mM NaCl stress, suggesting that the highly vacuolated root cortical cells of pumpkin roots could sequester more Na+, limit the radial transport of Na+ to the stele and thus restrict the transport of Na+ to the shoot. These results provide direct evidence for pumpkin roots with higher capacity to limit the transport of Na+ to the shoot than cucumber roots. 相似文献
17.
- Accumulation of NaCl in soil causes osmotic stress in plants, and sodium (Na+) and chloride (Cl?) cause ion toxicity, but also reduce the potassium (K+) uptake by plant roots and stimulate the K+ efflux through the cell membrane. Thus, decreased K+/Na+ ratio in plant tissue lead us to hypothesise that elevated levels of K+ in nutrient medium enhance this ratio in plant tissue and cytosol to improve enzyme activation, osmoregulation and charge balance.
- In this study, wheat was cultivated at different concentrations of K+ (2.2, 4.4 or 8.8 mm ) with or without salinity (1, 60 or 120 mm NaCl) and the effects on growth, root and shoot Na+ and K+ distribution and grain yield were determined. Also, the cytosolic Na+ concentration was investigated, as well as photosynthesis rate and water potential.
- Salinity reduced fresh weight of both shoots and roots and dry weight of roots. The grain yield was significantly reduced under Na+ stress and improved with elevated K+ fertilisation. Elevated K+ level during cultivation prevented the accumulation of Na+ into the cytosol of both shoot and root protoplasts. Wheat growth at vegetative stage was transiently reduced at the highest K+ concentration, perhaps due to plants' efforts to overcome a high solute concentration in the plant tissue, nevertheless grain yield was increased at both K+ levels.
- In conclusion, a moderately elevated K+ application to wheat seedlings reduces tissue as well as cytosolic Na+ concentration and enhances wheat growth and grain yield by mitigating the deleterious effects of Na+ toxicity.
18.
Two ionophores, monensin and salinomycin, increased total cell Na+ and ouabain-sensitive 86Rb+ uptake in cultures of smooth muscle cells from rat aorta. Monensin was used to produced graded increases in cell Na+ in order to assess the Na+ dependence of the Na+/K+ pump in the intact cell. The relationship between internal Na+ and ouabain-sensitive 86Rb+ uptake was hyperbolic (K1Na = 3 mM). Monensin did not stimulate 86Rb+ uptake in the absence of external Na+. Loading the cells with Na+ by exposing cultures to a K+-free medium for 3 hr maximally increased cell Na+ and ouabain-sensitive 86Rb+ uptake to the same extent as monensin. Total cell Na+ and pump activity in monensin-treated cells returned to the initial values after removing the ionophore. Monensin was then able to increase total cell Na+ and ouabain-sensitive 86Rb+ uptake to the same extent as the initial treatment with the ionophore. 相似文献
19.
Alkali cation selectivity of the wheat root high-affinity potassium transporter HKT1 总被引:19,自引:0,他引:19
Walter Gassmann Francisco Rubio Julian I. Schroeder 《The Plant journal : for cell and molecular biology》1996,10(5):869-882
The wheat root high-affinity K+ transporter HKT1 functions as a sodium-coupled potassium co-uptake transporter. At toxic millimolar levels of sodium (Na+), HKT1 mediates low-affinity Na+ uptake while potassium (K+) uptake is blocked. In roots, low-affinity Na+ uptake and inhibition of K+ uptake contribute to Na+ toxicity. In the present study, the selectivity among alkali cations of HKT1 expressed in Xenopus oocytes and yeast was investigated under various ionic conditions at steady state. The data show that HKT1 is highly selective for uptake of the two physiologically significant alkali cations, K+ and Na+ over Rb+, Cs+ and Li+. In addition, Rb+ and Cs+, and an excess of extracellular K+ over Na+, are shown to partially reduce or block HKT1-mediated K+-Na+ uptake. Furthermore, K+, Rb+ and Cs+ also effectively reduce outward currents mediated by HKT1, thereby causing depolarizations. In yeast, HKT1 can produce high-affinity Rb+ uptake at approximately 15-fold lower rates than for K+. Rb+ influx in yeast can be mediated by the ability of the yeast plasma membrane proton pump to balance the 35-fold lower HKT1 conductance for Rb+. A model for HKT1 activity is presented involving a high-affinity K+ binding site and a high-affinity Na+ binding site, and competitive interactions of K+, Na+ and other alkali cations for binding to these two sites. Possible implications of the presented results for physiological K+ and Na+ uptake in plants are discussed. 相似文献
20.
Interactions among Ca2+, Na+ and K+ in salinity toxicity: quantitative resolution of multiple toxic and ameliorative effects 总被引:5,自引:0,他引:5
Root elongation by wheat seedlings (Triticum aestivum
L. cv. Scout 66) was not inhibited by NaCl or KCl up to 130 mM in culture
solutions or by high Na+ (2 mg
g-1 FW) or K+ (4 mg
g-1 FW) in the root tissue, provided that
[Ca2+]>2 mM in the rooting medium. At [NaCl],
[KCl], or [mannitol] >250 mOs, root elongation was progressively
inhibited, irrespective of high [Ca2+]. In contrast,
shoot elongation was sensitive to any diminution of water potential, and
Ca2+ alleviated the toxicity only weakly. At solute
concentrations <250 mOs, the following interactions were observed.
Ca2+ alleviated Na+ and
K+ toxicity to roots by at least three separate
mechanisms. K+ was more toxic to roots than
Na+, but Na+ was more toxic
to shoots. Low levels of K+ relieved
Na+ toxicity, but low levels of
Na+ enhanced K+ toxicity.
Tissue concentrations of Na+ were reduced by
Ca2+ and K+ in the rooting
medium, and tissue concentrations of K+ were
enhanced by Ca2+ and Na+.
Several hypotheses relating to salinity toxicity can be evaluated, at least
for wheat seedlings. The osmoticant hypotheses (salinity intoxication
occurs because of diminished water potential) is true for shoots at all
salinity levels, but is true for roots only at high salinity. The
Ca2+-displacement hypothesis
(Na+ is toxic because it displaced
Ca2+ from the cell surface) is correct, but often of
minor importance. The K+-depletion hypothesis
(Na+ is toxic because it causes a loss of
K+ from plant tissues) is false. The
Cl--toxicity hypothesis (the apparent toxicity of
Na+ is induced by associated
Cl-) is false. The results indicate that, apart from
osmotic effects, high levels of Na+ in the rooting
medium and in the tissues are not toxic unless Ca2+
is also deficient, a condition probably leading to inadequate
compartmentation and excessive cytoplasmic accumulation. This study related
growth to ion activities at plasma-membrane surfaces. These activities were
computed by a Gouy-Chapman-Stern model then incorporated into non-linear
growth models for growth versus toxicants and ameliorants.Key
words: Calcium, potassium, salinity, sodium, toxicity
相似文献