共查询到20条相似文献,搜索用时 31 毫秒
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Eric Guisbert Daniel M. Czyz Klaus Richter Patrick D. McMullen Richard I. Morimoto 《PLoS genetics》2013,9(4)
The heat shock response (HSR) is essential to survive acute proteotoxic stress and has been studied extensively in unicellular organisms and tissue culture cells, but to a lesser extent in intact metazoan animals. To identify the regulatory pathways that control the HSR in Caenorhabditis elegans, we performed a genome-wide RNAi screen and identified 59 genes corresponding to 7 positive activators required for the HSR and 52 negative regulators whose knockdown leads to constitutive activation of the HSR. These modifiers function in specific steps of gene expression, protein synthesis, protein folding, trafficking, and protein clearance, and comprise the metazoan heat shock regulatory network (HSN). Whereas the positive regulators function in all tissues of C. elegans, nearly all of the negative regulators exhibited tissue-selective effects. Knockdown of the subunits of the proteasome strongly induces HS reporter expression only in the intestine and spermatheca but not in muscle cells, while knockdown of subunits of the TRiC/CCT chaperonin induces HS reporter expression only in muscle cells. Yet, both the proteasome and TRiC/CCT chaperonin are ubiquitously expressed and are required for clearance and folding in all tissues. We propose that the HSN identifies a key subset of the proteostasis machinery that regulates the HSR according to the unique functional requirements of each tissue. 相似文献
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昆虫的热休克反应和热休克蛋白 总被引:5,自引:1,他引:5
热休克(热激heatshock)是指短暂、迅速地向高温转换所诱导出的一种固定的应激反应。诱导该反应的温度在种与种之间有所不同。热休克反应最明显的特征是:伴随着正常蛋白质合成的抑制,一部分特殊蛋白质的诱导和表达增加,即为热休克蛋白(heatshockproteins,HSPs)。尽管热休克蛋白的合成也能被其它形式的应激反应所诱导,将它们认为是应激蛋白可能更恰当,但人们习惯上仍将这类蛋白质称为热休克蛋白。由于热休克反应和热休克蛋白是在果蝇(Drosophiliamelanogaster)中最初发现的,故在昆虫中,特别是果蝇等双翅目昆虫中研究得较深入… 相似文献
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Design of an optimal promoter involved in the heat‐induced transcriptional pathway in Arabidopsis,soybean, rice and maize
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Kyonoshin Maruyama Takuya Ogata Norihito Kanamori Kyouko Yoshiwara Shingo Goto Yoshiharu Y. Yamamoto Yuko Tokoro Chihiro Noda Yuta Takaki Hiroko Urawa Satoshi Iuchi Kaoru Urano Takuhiro Yoshida Tetsuya Sakurai Mikiko Kojima Hitoshi Sakakibara Kazuo Shinozaki Kazuko Yamaguchi‐Shinozaki 《The Plant journal : for cell and molecular biology》2017,89(4):671-680
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Annalisa Paradiso Guido Domingo Emanuela Blanco Alessio Buscaglia Stefania Fortunato Milena Marsoni Pasquale Scarcia Sofia Caretto Candida Vannini Maria Concetta de Pinto 《Plant, cell & environment》2020,43(11):2727-2742
Heat stress (HS), causing impairment in several physiological processes, is one of the most damaging environmental cues for plants. To counteract the harmful effects of high temperatures, plants activate complex signalling networks, indicated as HS response (HSR). Expression of heat shock proteins (HSPs) and adjustment of redox homeostasis are crucial events of HSR, required for thermotolerance. By pharmacological approaches, the involvement of cAMP in triggering plant HSR has been recently proposed. In this study, to investigate the role of cAMP in HSR signalling, tobacco BY-2 cells overexpressing the ‘cAMP-sponge’, a genetic tool that reduces intracellular cAMP levels, have been used. in vivo cAMP dampening increased HS susceptibility in a HSPs-independent way. The failure in cAMP elevation during HS caused a high accumulation of reactive oxygen species, due to increased levels of respiratory burst oxidase homolog D, decreased activities of catalase and ascorbate peroxidase, as well as down-accumulation of proteins involved in the control of redox homeostasis. In addition, cAMP deficiency impaired proteasome activity and prevented the accumulation of many proteins of ubiquitin-proteasome system (UPS). By a large-scale proteomic approach together with in silico analyses, these UPS proteins were identified in a specific cAMP-dependent network of HSR. 相似文献
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Marie-Odile Parat Marie-Jeanne Richard Alain Favier Jean-Claude Béani 《Biological trace element research》1998,65(3):261-270
Heat shock protein (HSP) synthesis results from various types of injury, including heat shock (HS) and some oxidants. The
intracellular signals leading to HSP synthesis are not yet fully elucidated. We have studied the influence ofNNN’N’-tetrakis(2-pyridylmethyl)ethylene diamine (TPEN), a metal chelator known to induce cellular zinc and copper deprivation,
on resistance to heat and on hsp70 synthesis in HaCaT keratinocytes. TPEN was shown to sensitize HaCaT cells to heat shock.
The effect of TPEN was neutralized by equimolar Zn2+. By the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and Western blotting characterization of
hsp70, it was shown that cultured HaCaT cells constitutively express the inducible form of hsp70. The application of TPEN
alone slightly increases the level of hsp70 but inhibits its induction by HS. This inhibitory effect is related to metal deprivation,
because it is eliminated when Cu2+ or Zn2+ ions are supplied together with TPEN. These results suggest that these metals are involved in the expression by keratinocytes
of a stress protein which has a protective action against environmental stress. 相似文献
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Changes in chlorophyll content, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) binding protein (RBP), Rubisco activase (RA), Rubisco large (LS) and small (SS) subunits, and electrolyte leakage were investigated in wheat leaf segments during heat stress (HS) for 1 h and for 24 h at 40 °C in darkness or in light, as well as after recovery from heat stress (HSR) for 24 h at 25 °C in light. The 24-h HS treatment in darkness decreased irreversibly photosynthetic pigments, soluble proteins, RBP, RA, Rubisco LS and SS. An increase in RA and RBP protein contents was observed under 24-h HS and HSR in light. This increase was in accordance with their role as chaperones and the function of RBP as a heat shock protein.This work was partially supported by Swiss National Science Foundation (Project 31-55289.98). 相似文献
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