Utilization of the hydrolytic activity in taxonomy of hyphal yeast ascomycetes

Species producing pseudohyphae and true hyphae and forming asci with endospores were included in the studied group of yeast-like microorganisms. These species belong taxonomically to various genera,i.e. Saccharomycopsis, Endomycopsis, Guilliermondiella, Arthroascus, Hormoascus etc. The groupCandida lipolytica was treated separately (Blagodarskaja and Kocková-Kratochvílová 1973). The verified phenotypes were compared using a numerical method and according to their hydrolytic activity.     

OnEndomyces,Endomycopsis and related yeast-like fungi

Strains in the culture collection of the Centraalbureau voor Schimmelcultures under the generic namesEndomyces andEndomycopsis were compared with related genera of filamentous and yeast-like fungi. The genusEndomyces is accepted for ascigerous states ofGeotrichum; 6 species are keyed out and discussed. The genus nameEndomycopsis is rejected as it is an obligate synonym ofSaccharomycopsis. ForEndomyces javanensis, Endomycopsis platypodis andSaccharomycopsis synnaedendra the new generaArthroascus, Hormoascus andBotryoascus are proposed; the genusGuilliermondella is reintroduced. Briefly discussed are the generaSchizosaccharomyces, Eremascus, Dipodascus, Ascoidea, Cephaloascus, Ambrosiozyma andCrebrothecium. A key is given for the genera of yeast-like “ascomycetes” with septate hyphae. Some species described asGeotrichum are transferred to other genera.     

中国筇竹属植物志资料

筇竹属是近年来新建立的一个属。本属原发表时仅有3种,标本採自四川、云南、贵州及湖北西部。以后又陆续发表了4种。作者在编写中国植物志筇竹属的过程中,对本属又做了初步整理,其中包括一新种及一新变型也在此一并发表。本文引证的标本均藏于本院标本室(代号SWFC)。     

基于RAPD技术探讨黄精属部分药用植物系统位置

目的：从分子水平探讨黄精属部分药用植物间的系统位置。方法：采用RAPD分子标记方法,以同科万寿竹属植物宝铎草（Disporum sessile D．Don）为外类群,对百合科黄精属19批（6种）药用植物进行基因组DNA的多态性分析,通过聚类分析探讨黄精种属间亲缘关系。结果：筛选出7条随机引物,聚类结果表明,黄精属植物在属级分类上特征明显,但属内分类有交叉。结论：RAPD技术可以为黄精属的分类和种的鉴别提供一定的理论依据。     

Identification of dimethyl disulfide-forming bacteria isolated from activated sludge

Twenty-four strains with high dimethyl disulfide (DMDS)-forming ability were isolated from activated sludge and identified to the genus level. These bacteria were classified into four groups (A, B, C, and D) by the API ZYM System (API System S.A., Montalieu, France). Group A (three strains) was identified as genus Lactobacillus by the API 20B System, by the method of Cowan and Steel, and by production of lactic acid as confirmed by gas-liquid chromatography. Group B (eight strains) was identified as genus Corynebacterium by API 20B and the Cowan and Steel method. Group C (one strain) was suggested to belong to genus Corynebacterium by the API 20B System. Group D (12 strains) was identified as genus Pseudomonas or Alcaligenes by the API 20B System, as genus Alcaligenes by the Cowan and Steel method, and as Achromobacter group Vd by the API 20NE System. However, on the basis of guanine-plus-cytosine contents in DNA and form of flagella, these strains were identified as genus Pseudomonas. Formation of DMDS from DL-methionine and S-methyl-L-cysteine was tested. DMDS-forming bacteria isolated from activated sludge formed DMDS from both precursors. In genus Pseudomonas, P. aeruginosa could not form DMDS from either precursor, but P. acidovorans, P. alcaligenes, P. pseudoalcaligenes, and P. testosteroni formed DMDS. In genus Alcaligenes, A. denitrificans subsp. xylosoxydans, A. denitrificans subsp. denitrificans, A. faecalis, and A. odorans formed DMDS from both precursors. Achromobacter group Vd formed DMDS from S-methyl-L-cysteine, but could not from DL-methionine.     

进口竹藤中竹长蠹五近似种的鉴别(鞘翅目:长蠹科)

介绍了近年从进口竹藤检疫中多次截获的5种竹长蠹:双窝竹长蠹 Dinoderus bifoveolatus (Wollaston)、小竹长蠹D. brevis Horn、日本竹长蠹D. japonicus Lesne、竹长蠹D. minutus Fabricius及小点竹长蠹D. ocellari Stephens,分别介绍了头、前胸背板、鞘翅、腹面及足的主要鉴别特征,并编制了这5个近似种的检索表.     

Complete genome sequence of Deinococcus maricopensis type strain (LB-34)

Deinococcus maricopensis (Rainey and da Costa 2005) is a member of the genus Deinococcus, which is comprised of 44 validly named species and is located within the deeply branching bacterial phylum Deinococcus-Thermus. Strain LB-34(T) was isolated from a soil sample from the Sonoran Desert in Arizona. Various species of the genus Deinococcus are characterized by extreme radiation resistance, with D. maricopensis being resistant in excess of 10 kGy. Even though the genomes of three Deinococcus species, D. radiodurans, D. geothermalis and D. deserti, have already been published, no special physiological characteristic is currently known that is unique to this group. It is therefore of special interest to analyze the genomes of additional species of the genus Deinococcus to better understand how these species adapted to gamma- or UV ionizing-radiation. The 3,498,530 bp long genome of D. maricopensis with its 3,301 protein-coding and 66 RNA genes consists of one circular chromosome and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.     

The systematics of Australian Daphnia (Cladocera: Daphniidae). Multivariate morphometrics

Forty nine characters were measured in each of 76 male and 200 female Daphnia including specimens from all eleven taxa described from the genus in Australia, except D. jollyi. Separate multivariate analyses (cluster, principal components and discriminant) of males and females each revealed only five morphological groups in the genus corresponding to the species D. occidentalis, D. lumholtzi, D. cephalata, D. nivalis, and a conglomerate of all other taxa described from the D. carinata complex, D. carinata (sensu lato). Interpretation of the male results was straightforward, but that for females was not because of the occurrence of an environmentally induced seasonal change in morphology (cyclomorphosis) in females. Log transformations of the female data successfully isolated the effects of cyclomorphosis. The seasonal morphs formed two groups which were further differentiated into taxonomic groups.     

Phylogenetic position of the Dalmatian genus Phoxinellus and description of the newly proposed genus Delminichthys (Teleostei: Cyprinidae)

The Dalmatian cyprinid genus Phoxinellus is characterized by reductive characters most likely associated with the environmental conditions of small karstic streams, where all species of this genus occur. Based on 33 morphological traits, nuclear and mtDNA sequences Phoxinellus was found to be paraphyletic and included three not closely related monophyletic units. The scientific name Phoxinellus should therefore be restricted to species having plain coloration, small or absent postcleithrum, no genital papilla and an almost entirely naked body such as P. alepidotus, P. dalmaticus, and P. pseudalepidotus. Species that also have a small or absent postcleithrum and no genital papilla but display a dark stripe from the head to the caudal peduncle, and are entirely covered by distinct, not overlapping scales should be positioned closely to Telestes. Thus, we suggest inclusion of Phoxinellus croaticus, P. fontinalis and Paraphoxinus metohiensis in the genus Telestes. The Phoxinellus species that have a irregularly spotted color pattern, a large postcleithrum, an increased number of precaudal anal-fin pterygiophores, and a large genital papilla in females represent its own evolutionary line closely related to the Balkan species of Pseudophoxinus. For this monophyletic group, we propose to introduce a new genus: Delminichthys. This genus includes the species D. adspersus, D. ghetaldii, D. krbavensis and D. jadovensis.     

Use of nuclear and mitochondrial DNA PCR and sequencing for molecular identification of Diphyllobothrium isolates potentially infective for humans

Tapeworms of the genus Diphyllobothrium (Cobold, 1858) are widely distributed all around the world and some of them are agents of human diphyllobothriasis. Approximately 50 species have been described within the Diphyllobothrium genus but only 13 are human pathogens. Species identification by using morphological criteria is very difficult. We determined the value of 18S ribosomal RNA gene, internal transcribed spacer (ITS) and cytochrome c oxidase subunit 1 gene (COI) sequences to differentiate between Diphyllobothrium isolates. Sequences from 18 isolates (larvae or adults) of D. latum, D. nihonkaiense, D. ditremum, D. dentriticum and D. stemmacephalum species were obtained. COI region sequences analysis was clearly more discriminative than those of the ITS1 and 18S rRNA and was a useful tool for identifying specimens.     

中国云南臭蚁属二新种记述 (膜翅目:蚁科)

描述了中国云南省南部热带雨林臭蚁属Dolichoderus Lund 2新种.目前为止已在中国记载该属昆虫10种:西伯利亚臭蚁D.sibiricus Emery、鞍背臭蚁D.sagmanotus Xu,sp.nov.、费氏臭蚁D.feae Emery、黑腹臭蚁D.taprobanae(Smith)、平背臭蚁D.flatidorsus Zhou、毛臭蚁D.pilosus Zhou、邻臭蚁D.affinis Emery、凹头臭蚁D.incisus Xu、黑可可臭蚁D.thoracicus(Smith)、鳞结臭蚁D.squamanodus Xu,sp.nov..提供了10个中国已知种的工蚁分种检索表.     

T-specific D17Leh66 DNA elements in family Muridae

Blot-hybridization analysis with the use of the t-specific probe D17Leh66 has been used to study DNA of various representatives of family Muridae. Hamsters from genus Phodopus have no homologs of this probe, whereas African rats from genus Lophuromys have some homologous elements. This indicates that sequence Dl7Leh66 is ancient and was probably present in the common ancestor of family Muridae.     

Description of two new species of Diaphanosoma Fischer, 1850 (Crustacea, Branchiopoda, Sididae) from the United States and Canada and species richness of the genus in North America

Investigation of a small number of samples with representatives of the genus Diaphanosoma from the cladoceran collection of the National Museum of Natural History, Smithsonian Institution (Washington, D.C., U.S.A.) has revealed two new species, D. freyi and D. heberti, from Louisiana–Missouri and Newfoundland, respectively. The former looks like one of the most primitive species of the genus. Two other species, D. fluviatile and D. brevireme, are recorded from the southern United States for the first time. Members of the D. brachyurum and D. birgei species groups, which are morphologically variable and need further detailed investigations, were represented in the bulk of the material studied. The species richness of the genus in North America appears to be high and its composition is obviously complex but remains little studied.     

An ustilaginomycetous yeast,Pseudozyma graminicola sp. nov., isolated from the leaves of pasture plants

Two strains belonging to a novel anamorphic species, Pseudozyma graminicola, were isolated from the leaves of herbaceous plants in the Moscow region (Russia). This species was genetically distinct from all known Pseudozyma species, based on sequence divergence in the D1/D2 domains of the large subunit rDNA and the ITS region. It is related phylogenetically to species of the genus Sporisorium (Ustilaginaceae, Ustilaginales). Physiological characteristics distinguishing this novel species from the other species of the genus Pseudozyma are presented.     

中国管伪叶甲属研究(鞘翅目:伪叶甲科)(英文)

把管伪叶甲属DonaciolagriaPic从伪叶甲亚科Lagriinae移至突伪叶甲亚科Statirinae，首次记载了该属的雌虫，重新描述了该属及腿管伪叶甲D．femoralis（Borchmann），描述了金绿管伪叶甲D．viridimetallica和黄胫管伪叶甲D．flavitibialis两新种，列出了中国种类检索表，绘制了22个原始图。     

Three new species of the leafhopper genus Dayus Mahmood from China (Hemiptera,Cicadellidae,?Typhlocybinae,Empoascini)

Three new species of the Oriental empoascine leafhopper genus Dayus Mahmood are described from China: D. bifurcatus sp. n., D. trifurcatus sp. n. and D. serratus sp. n. A key to distinguish all Chinese species of the genus is provided.     

Systematic position and review of Deinopteroloma Jansson, 1946, with descriptions of four new species (Coleoptera, Silphidae and Staphylinidae (Omaliinae))

Abstract. The genus Deinopteroloma Jansson 1946 is transferred from the family Silphidae to the family Staphylinidae and assigned to the subfamily Omaliinae, tribe Anthophagini. The genus Mathrilaeum Moore 1966 is shown to be congeneric with Deinopteroloma and considered a junior synonym. The genus Deinopteroloma is redescribed and its position in the Anthobium group of genera is discussed. Nine species are recognized, five in the Nepal Himalayas, two in northeastern Burma and two on the Pacific coast of North America. A key for identification is given. Each species is described and illustrated and all available bionomic and distributional data are presented. Deinopteroloma crenatum, D.spectabile, D.egregium and D.insigne , all from the Nepal Himalayas, are described as new and Lathrimaeum subcostatum Maklin, L.pictum Fauvel and L.notabile Cameron are transferred in Deinopteroloma (comb.nov.). Lectotypes are designated for D.subcostatum (Mäklin), D.humerale (Casey), D.pictum (Fauvel) and D.notabile (Cameron).     

中国西南六种广义拂子茅属(禾本科)植物的染色体数目

广义拂子茅属（Calamagrostis）是一个世界温带广布的大属,有些作者又分为拂子茅属和野青茅属,但近期的研究表明处理为一个属较为合适。中国共有37种广义拂子茅属植物,但至今没有任何染色体的研究。本文报道了其中产于中国西南6种野青茅的染色体数目,其中Deyeuxia petelotii 4个居群,D．diffusa,D．moupinemis,D．nivicola和D．flvens各一个居群都是四倍体（2n=4x=28）,D．neglecta为六倍体（2n=6x=42）。根据广义拂子茅属植物染色体倍性特征,该属植物中至今未发现二倍体,四倍体是该属中倍性最低和最普遍的,广义拂子茅属的演化很可能是在四倍体的水平上进行的。由于以上几个四倍体种均是狭域分布的类群,所以可能是由四倍体的祖先隔离分化形成的。