New insights into Late Bronze Age settlement and farming activity in the southern Burren,western Ireland

Vegetation History and Archaeobotany - This paper is a palaeoenvironmental investigation into prehistoric farming activity in the southern Burren, a distinctive karst landscape in western...     

Plant Resources from the Bronze Age and the first Iron Age in the northwestern arc of the Mediterranean Basin

This paper updates the question of plant resources during the Bronze Age and First Iron Age in the northwestern Mediterranean Basin. Among the cereals, six-row hulled barley is dominant throughout the territory, whereas naked and hulled wheats take on greater or lesser roles from region to region. Millet cultivation developed during the Bronze Age and became widespread in the First Iron Age. Apart from cereals, pulses, oil species and fruit appear to be secondary. Results from the study of archaeobotanical remains on wetland sites, however, lead us to question this finding, as oil plants and fruits are much better represented in waterlogged conditions. The cultivation of vine began in the First Iron Age. In spite of a number of characteristics common to plants throughout the study area, regional differences, evident in the Bronze Age, seem to dissipate in the First Iron Age.     

Mortality changes in Central European populations from Bronze Age to Iron Age: Comparative analysis

The influence of cultural factors on the biological state, of prehistorical populations inhabiting Central Europe in the Bronze and Iron Ages have been analyzed. To achieve this goal the author studied changes in mortality structure of human groups said to belong to two various archeological cultures.     

Dendrochronological analyses of wood samples from a Late Bronze to early Iron Age site at Lake Luokesa,Lithuania

Tree rings of 184 archaeological wood samples from two Late Bronze to early Iron Age lake sites at Lake Luokesa (Luokesai e?eras), Lithuania, Moletai district, were analyzed. Despite severe difficulties with synchronization, Pinus (pine), Quercus (oak) and Alnus (alder) yielded some cross-datable series. The general picture is that the settlers chose small trees as timber, which they used in their natural round shape. The trees did not grow in homogeneous even-aged stands, but show very different ages and growth levels. Despite the generally low numbers of tree rings in the individual samples, the strong archaeological framework allowed cross-dating of some series and the building of chronologies for single structures. Based on these attempts, a 90 year long first floating chronology of the settlement structures is presented. Luokesa Site 2 (L2) was mainly built within the relative year 53. Luokesa Site 1 (L1) was certainly in use from the relative year 74 onwards. All fences at L1 show their main building activity in the relative year 81, four years after the main building activities in the village itself. It can be concluded that the settlement L1 was in use for at least 16 years. Because of the lack of a standard dendrochronological curve for the Baltic region, wiggle-matching was applied to obtain an absolute date for both settlements. The data clearly show that all samples relate to the Late Bronze–early Iron Age. The period where all wiggle matching results overlap is the period between 625 and 535 bc (the 2σ ranges are given). Based on the dating, duration and timber characteristics of the occupation, comparisons with Polish early Iron Age sites are made, which indicate a close resemblance in terms of wood use and settlement concept.     

Genetic Continuity of Bronze Age Ancestry with Increased Steppe-Related Ancestry in Late Iron Age Uzbekistan

Although Uzbekistan and Central Asia are known for the well-studied Bronze Age civilization of the Bactria–Margiana Archaeological Complex (BMAC), the lesser-known Iron Age was also a dynamic period that resulted in increased interaction and admixture among different cultures from this region. To broaden our understanding of events that impacted the demography and population structure of this region, we generated 27 genome-wide single-nucleotide polymorphism capture data sets of Late Iron Age individuals around the Historical Kushan time period (∼2100–1500 BP) from three sites in South Uzbekistan. Overall, Bronze Age ancestry persists into the Iron Age in Uzbekistan, with no major replacements of populations with Steppe-related ancestry. However, these individuals suggest diverse ancestries related to Iranian farmers, Anatolian farmers, and Steppe herders, with a small amount of West European Hunter Gatherer, East Asian, and South Asian Hunter Gatherer ancestry as well. Genetic affinity toward the Late Bronze Age Steppe herders and a higher Steppe-related ancestry than that found in BMAC populations suggest an increased mobility and interaction of individuals from the Northern Steppe in a Southward direction. In addition, a decrease of Iranian and an increase of Anatolian farmer-like ancestry in Uzbekistan Iron Age individuals were observed compared with the BMAC populations from Uzbekistan. Thus, despite continuity from the Bronze Age, increased admixture played a major role in the shift from the Bronze to the Iron Age in southern Uzbekistan. This mixed ancestry is also observed in other parts of the Steppe and Central Asia, suggesting more widespread admixture among local populations.     

Food storage in two Late Bronze Age caves of Southern France: palaeoethnobotanical and social implications

This paper presents new archaeobotanical results from two previously studied Late Bronze Age caves situated in Southern France, Balme Gontran and Baume Layrou. At each site a thick black layer, characterised by a very high density of charred seeds, is shown to be composed of the remains of burnt crop stores. In Baume Layrou a small proportion of desiccated plant remains was preserved in addition to the bulk of carbonised material. In Balme Gontran, Triticum spelta and Panicum miliaceum predominated and were independently stored. Lens culinaris, Vicia faba and Setaria italica were secondary species of some importance and could have been stored as well. Storage at Baume Layrou was above all composed of hulled Hordeum vulgare, Triticum spelta and P. miliaceum. Other possibly stored species were Triticum aestivum/durum/turgidum, T. dicoccon and V. faba. It seems that most of the crops were grown in pure stands, with the exception of S. italica and Triticum monococcum which may have been mixed in small proportions with common millet and emmer respectively. Crops were stored in ceramic vessels, probably also in bags and wooden containers like baskets. Millet grains were stored in their husks while glume wheats were dehusked. Dehusking before storage does not seem to have been the common practice at the time. It seems moreover rather unsuitable for grain storage in caves. In Baume Layrou a small proportion of cereal kernels had started to germinate, presumably due to the humidity of the cave. We are making the assumption that the caves were not used for usual long term storage but to store food supplies for a small group of people who intended to live here for a short period, perhaps taking refuge during disturbed times. Crops could have been dehusked to reduce the weight and volume of the load to transport to the caves on steep and difficult paths.     

Environment and agriculture of the transitional period from the Late Bronze to early Iron Age in the eastern Baltic: an archaeobotanical case study of the lakeshore settlement Luokesa 1, Lithuania

Prehistoric agriculture and vegetation in Lithuania have so far been reconstructed largely using palynological data. This paper reports the archaeobotanical investigation of macroremains from the Late Bronze-early Iron Age (LBA–EIA) lakeside settlement Luokesa 1 (L1) in eastern Lithuania, with the aim of elucidating the settlement’s history and crop diversity. The single phase settlement was fortified with an onshore palisade and is dated between 625 and 535 cal. bc. Samples were taken along a land to lake transect, and in the centre of L1. The stratigraphy consisted of three distinct layers: lake marl, cultural deposit and sandy limnic sediment on top. The plant spectrum shows that L1 was constructed on an exposed morainic shoal. It was surrounded by woodland, meadows, fields/gardens, ruderal habitats and riverine vegetation. Accumulated cultural deposits consisted mainly of manure (litter, fodder and dung of sheep/goat), with rubbish, sweepings from the houses or remains of the on-site vegetation. The crops were Panicum miliaceum, Triticum spelta, T. dicoccon, Hordeum vulgare s.l., Pisum sativum and Camelina sativa, the latter being the first prehistoric evidence in Lithuania. The trophic state of the lake increased during the occupation period. After the abandonment of the settlement the ruins decayed until the lake flooded the site. The results are discussed in a broader context of the LBA/EIA cultures in northern Central Europe.     

Echoes from the past: New insights into the early hominin cochlea from a phylo-morphometric approach

We investigate cochlear variation, an indirect evidence of auditory capacities among early hominins and extant catarrhine species, in order to assess (i) the phylogenetic signal of relative external cochlear length (RECL) and oval window area (OWA), (ii) the evolutionary model with the highest probability of explaining our observed data, (iii) some hominin ancestral nodes for RECL and OWA. RECL has a high phylogenetic signal under a Brownian motion model, and is closely correlated with body mass. Our model-based method has the advantage over parsimony-based methods of incorporating branch lengths in a phylo-morphospace, and this shows RECL shifted towards significantly higher values at the Homo erectus-Homo sapiens node. We also observe that the StW 53 and KB 6067 fossil specimens from Sterkfontein and Kromdraai likely represent one or two distinct, smaller-bodied and less derived hominin form(s) compared to Paranthropus specimens represented at Swartkrans.     

New insights into the allergenicity of tropomyosin: a bioinformatics approach

The invertebrate panallergen tropomyosin is a protein with an extremely simple folding. This makes it a perfect target for investigating structural differences between invertebrate and vertebrate tropomyosins, which are not considered allergenic. Phylogenetic and sequence analyses were conducted in order to explore the differences in primary structure between several tropomyosins and to promote an experimental development in the field of food allergy, based on the study of tropomyosin. The phylogenetic analyses showed that tropomyosin is a useful evolutionary marker. The phylogenetic trees obtained with tropomyosin were not always phylogenetically correct, but they might be useful for allergen avoidance by tropomyosin allergic individuals. Sequence analyses revealed that the probability of alpha helix folding in invertebrate tropomyosins was lower than in all the studied vertebrate ones, except for the Atlantic bluefin tuna Thunnus thynnus tropomyosin. This suggested that the lack of alpha helix folding may be involved in the immunogenicity of tropomyosins. More specifically, the regions adjacent to the positions 133–135 and 201 of the invertebrate tropomyosins, presented lower probability of alpha helix folding than those of vertebrates and are candidates to be responsible for their allergenicity.     

Future benefits from biological nitrogen fixation: An ecological approach to agriculture

Strategies for the enhancement and exploitation of biological nitrogen fixation are assessed with attention to the likely timescales for realization of benefits in agriculture. Benefits arising from breeding of legumes for N₂-fixation and rhizobial strain selection have less potential to increase inputs of fixed N than alleviation of environmental stresses or changes in farming systems to include more legumes. Genetic engineering may result in substantial enhancement of N₂-fixation, particularly if the ability to fix N₂ is transferred to other crops but these are long-term goals. Immediate dramatic enhancements in inputs from N₂-fixation are possible simply by implementation of existing technical knowledge. Apart from the unfortunate political and economic barriers to the use of agricultural inputs, better communication between researchers and farmers is required to ensure proper focus of research and development of appropriate technologies. Legumes must be considered within the context of the farming systems within which they are grown and not in isolation. Proper integration of legumes requires a good understanding of the role of the legume within the system and a better understanding of the relative contributions of N sources and of the fates of fixed N.     

Land-use patterns and cultural change in the Middle to Late Bronze Age in Ireland: inferences from pollen records

In Ireland, the Middle to Late Bronze Age (1500–600 cal b.c.) is characterised by alternating phases of prolific metalwork production (the Bishopsland and Dowris Phases) and apparent recessions (the Roscommon Phase and the Late Bronze Age-Iron Age transition). In this paper, these changes in material culture are placed in a socio-economic context by examining contemporary settlement and land-use patterns reconstructed from the pollen record. The vegetation histories of six tephrochronologically linked sites are presented, which provide high-resolution and chronologically well-resolved insights into changes in landscape use over the Middle to Late Bronze Age. The records are compared with published pollen records in an attempt to discern if there are trends in woodland clearance and abandonment from which changes in settlement patterns can be inferred. The results suggest that prolific metalworking industries correlate chronologically with expansion of farming activity, which indicates that they were supported by a productive subsistence economy. Conversely, declines in metalwork production occur during periods when farming activity was generally less extensive and perhaps more centralised, and it is proposed that disparate socio-economic or political factors, rather than a collapse of the subsistence economy, lie behind the demise of metalworking industries.     

Living in wetlands in the southeastern Baltic region during the Late Bronze to early Iron Age: the archaeological context of the Luokesa lake settlements

The prehistoric lake settlement tradition is spread far beyond the region of the Alps, and it has been known for a long time that lake settlements are not a characteristic of one particular area. The present paper presents the results of the first comprehensive, interdisciplinary investigations of such types of sites in Lithuania, the two lake settlements at Lake Luokesa (Luokesai e?eras). They were excavated with underwater archaeology techniques between 2000 and 2011. They are Late Bronze to early Iron Age transition (LBA–EIA) in date and were probably built and inhabited during a short period between 625 and 535 cal bc. The excavated archaeological material contains a wealth of well-preserved wooden architectural details and other organic materials. Therefore, the importance and unique character of these sites is beyond question. This paper gives an overview of the history of research on lake settlements in northeastern Europe. In addition, the archaeological material of the LBA–EIA settlements at Lake Luokesa is evaluated in the context of the other lake settlements in the southeastern Baltic region. Existing hypotheses and interpretations of the origins, development and use of the lake settlements of this region are discussed. All the investigations which have been done, including archaeobotany, palynology, dendrochronology and geoarchaeology, provide data for a well-grounded interpretation and reconstruction of the Luokesa lake settlements.     

Decoupling of local and regional dominance in trilobite assemblages from northwestern Argentina: new insights into Cambro‐Ordovician ecological changes

Components of biodiversity are strongly scale dependent, but the relative importance of the patterns that operate at different scales and the links between them have been overlooked. To disentangle the ecological structure of Cambro‐Ordovician trilobite assemblages from the Argentine Cordillera Oriental at different scales, we explore patterns of abundance, dominance and occupancy across the onshore–offshore profile, and through three time intervals: Furongian, earliest Late Tremadocian (Tr2), latest Middle Floian–earliest Late Floian (Fl2–Fl3). At the regional scale, single taxa are overwhelming dominant in the Furongian (Parabolina) and in the earliest Late Tremadocian (Leptoplastides). Several dominants occur in the Floian, but just one (Famatinolithus) attains high occupancy and, rarely, high dominance. In contrast, only the Furongian records highly dominated local assemblages, whereas dominance distinctly decreases among Tr2 and Fl2–Fl3 ones. Thus, when both scales of analysis are combined, an unexpected scenario becomes evident: Tr2 assemblages resemble those of the Furongian at the regional scale, but mirror those of the Floian at the local scale. These results highlight a decoupling in local versus regional structures triggered by an earlier switch in dominance in local communities and a delayed change at the regional scale. Interestingly, this decrease in local dominance matches previous analyses accounting for a coeval step‐up in local evenness, suggesting that the Tr2 appears as a pivotal interval in the reorganization of communities in the Cordillera Oriental. This scenario emphasizes that biogeographical regions witnessed different regional‐scale processes, and suggests that scaling local and regional patterns provides new insights to unravel the history of biodiversity among benthic communities.     

New insights into the paleobiogeography of the Early Ordovician graptolite fauna of northwestern Argentina

A multivariate analysis was used to determine the faunistic relationships of the northwestern Argentinean graptolite faunas of Floian age to faunas from Baltica, Avalonia, Laurentia and SW China. A statistical analysis at the species level of the five geographic regions for the Lower Floian was performed with the classic Jaccard's index. The resulting affinity dendrogram shows stronger relationships between Early Floian graptolite faunas of northwestern Argentina and those from Baltica, with less obvious similarities to Great Britain (Avalonia) faunas and only weak affinities to North American (Laurentia) and Chinese (SW China) graptolite faunas. The statistical analysis confirms the paleobiogeographic relationships previously observed in other areas of the Cordillera Oriental, and supports the hypothesis that during the Early Ordovician, northwestern Argentina was located at middle to high latitudes, corresponding to the Atlantic Faunal Realm of cold water graptolite biofacies. The studied graptolite material from the Quinilicán and Agua Chica sections is preserved in shales and siltstones interbedded with fine to medium-grained sandstones corresponding to the lower part of the Acoite Formation. The biostratigraphic implications of the associated graptolites are briefly discussed, and Trichograptus dilaceratus (Herrmann), Acrograptus gracilis (Törnquist), Expansograptus latus (T.S. Hall) and Corymbograptus v-fractus tullbergi (Monsen) are described from the Argentine Cordillera Oriental for the first time.     

New insights into autophagic cell death in the gypsy moth Lymantria dispar: a proteomic approach

Autophagy is an evolutionary ancient process based on the activity of genes conserved from yeast to metazoan taxa. Whereas its role as a mechanism to provide energy during cell starvation is commonly accepted, debate continues about the occurrence of autophagy as a means specifically activated to achieve cell death. The IPLB-LdFB insect cell line, derived from the larval fat body of the lepidoptera Lymantria dispar, represents a suitable model to address this question, as both autophagic and apoptotic cell death can be induced by various stimuli. Using morphological and functional approaches, we have observed that the culture medium conditioned by IPLB-LdFB cells committed to death by the ATPase inhibitor oligomycin A stimulates autophagic cell death in untreated IPLB-LdFB cells. Moreover, proteomic analysis of the conditioned media suggests that, in IPLB-LdFB cells, oligomycin A promotes a shift towards lipid metabolism, increases oxidative stress and specifically directs the cells towards autophagic activity. Electronic Supplementary Material  The online version of this article (doi:) contains supplementary material, which is available to authorized users. This work was supported by an F.A.R. grant from the University of Modena and Reggio Emilia (D.M. and E.O.) and by an “Experimental approaches to the study of evolution” grant from the Department of Animal Biology of the University of Modena and Reggio Emilia (D.M.).     

New insights into fragile X syndrome: from molecules to neurobehaviors

Fragile X syndrome - a common form of inherited mental retardation - is caused by the loss of the fragile X mental retardation 1 protein (FMRP). FMRP is an RNA-binding protein which forms a messenger ribonucleoprotein (mRNP) complex that associates with translating polyribosomes. It has been proposed that FMRP is involved in synaptic plasticity through the regulation of mRNA transportation and translation. Recent advances in the identification of the mRNA ligands that are bound by FMRP, the RNA sequence and structure required for FMRP-RNA interaction, and the physiological consequences of FMRP deficiency in the brain are important steps towards understanding the molecular pathogenesis of fragile X syndrome, and learning and memory in general.     

New insights into fish swimming: a proteomic and isotopic approach in gilthead sea bream

Moderate exercise enhances fish growth, although underlying physiological mechanisms are not fully known. Here we performed a proteomic and metabolic study in white (WM) and red (RM) muscle of gilthead sea bream juveniles swimming at 1.5 body lengths per second. Continuous swimming for four weeks enhanced fish growth without increasing food intake. Exercise affected muscle energy stores by decreasing lipid and glycogen contents in WM and RM, respectively. Protein synthesis capacity (RNA/protein), energy use (estimated by lipid-δ(13)C and glycogen-δ(13)C), and enzymatic aerobic capacity increased in WM, while protein turnover (expressed by δ(15)N-fractionation) did not change. RM showed no changes in any of these parameters. 2D-PAGE analysis showed that almost 15% of sarcoplasmic protein spots from WM and RM differed in response to exercise, most being over-expressed in WM and under-expressed in RM. Protein identification by MALDI-TOF/TOF-MS and LC-MS/MS revealed exercise-induced enhancement of several pathways in WM (carbohydrate catabolism, protein synthesis, muscle contraction, and detoxification) and under-expression of others in RM (energy production, muscle contraction, and homeostatic processes). The mechanism underpinning the phenotypic response to exercise sheds light on the adaptive processes of fish muscles, being the sustained-moderate swimming induced in gilthead sea bream achieved mainly by WM, thus reducing the work load of RM and improving swimming performance and food conversion efficiency.     

Vegetation change during the Bronze Age studied in a multi-proxy approach: use of wood linked to charcoal analysis

During recent archaeological excavations in the alpine valley of Montafon, western Austria, a Bronze and early Iron Age settlement cluster located at about 1,000 m a.s.l. was excavated. The human impact on the woodland resulting from these prehistoric settlement activities has been evaluated by the analysis of charred plant macro remains from cultural layers from a hilltop settlement site and two other close-by settlements, all of them encompassing the Early and Middle Bronze Age (19th to 15th century cal. b.c.) and early Iron Age (6th/5th century cal. b.c.). Charred seeds and fruits have provided information on the supply of foodstuff while charcoal (anthracological) analyses of firewood have revealed the use of wood and consequently the changes in local woods. The latter analyses suggest that the spruce-fir woodland (Piceeto-Abietetum) was gradually cleared from the Early Bronze Age. During the Middle Bronze Age large amounts of Pinus sylvestris (pine), Betula (birch), Corylus avellana (hazel) and Sorbus (rowan) with some Picea abies (spruce) characterized the woods, and early succession stages indicate clearings. These anthracological studies are corroborated by pollen studies disclosing clearings in the woods since the Early Bronze Age, which gradually expanded during the Middle Bronze Age. Furthermore, several charcoals from a Middle Bronze Age hearth seem to be of the same age, and the pattern of their annual growth-rings suggests the pollarding of broadleaved trees.