共查询到20条相似文献,搜索用时 31 毫秒
1.
Xue‐Qin Zeng Wah Soon Chow Li‐Juan Su Xin‐Xiang Peng Chang‐Lian Peng 《Physiologia plantarum》2010,138(2):215-225
Ten anthocyanin components have been detected in roots of purple sweet potato (Ipomoea batatas Lam.) by high‐performance liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry. All the anthocyanins were exclusively cyanidins or peonidin 3‐sophoroside‐5‐glucosides and their acylated derivatives. The total anthocyanin content in purple sweet potato powder obtained by solid‐phase extraction was 66 mg g?1. A strong capacity of purple sweet potato anthocyanins (PSPA) to scavenge reactive oxygen species (superoxide, hydroxyl radical) and the stable 1,1‐diphenyl‐2‐picrylhydrazyl organic free radical was found in vitro using the electron spin resonance technique. To determine the functional roles of anthocyanins in leaves in vivo, for the first time, supplemental anthocyanins were infiltrated into leaves of Arabidopsis thaliana double mutant of the ecotype Landsberg erecta (tt3tt4) deficient in anthocyanin biosynthesis. Chlorophyll fluorescence imaging showed that anthocyanins significantly ameliorated the inactivation of photosystems II during prolonged high‐light (1300 µmol m?2 s?1) exposure. Comet assay of DNA revealed an obvious role of supplemental PSPA in alleviating DNA damage by high light in leaves. Our results suggest that anthocyanins could function in vitro and in vivo to alleviate the direct or indirect oxidative damage of the photosynthetic apparatus and DNA in plants caused by high‐light stress. 相似文献
2.
Overexpression of the IbMYB1 gene in an orange‐fleshed sweet potato cultivar produces a dual‐pigmented transgenic sweet potato with improved antioxidant activity
下载免费PDF全文
![点击此处可从《Physiologia plantarum》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Sung‐Chul Park Yun‐Hee Kim Sun Ha Kim Yu Jeong Jeong Cha Young Kim Joon Seol Lee Ji‐Yeong Bae Mi‐Jeong Ahn Jae Cheol Jeong Haeng‐Soon Lee Sang‐Soo Kwak 《Physiologia plantarum》2015,153(4):525-537
3.
4.
Ying Zhou Dong Guo Jing Li Jun Cheng Hui Zhou Chao Gu Sue Gardiner Yue-Peng Han 《Tree Genetics & Genomes》2013,9(1):265-278
The usual red color of young leaves of peach (Prunus persica f. atropurpurea) is due to the accumulation of anthocyanin. Real-time PCR analysis revealed a strong correlation between the expression levels of anthocyanin biosynthetic genes and anthocyanin content in leaves at different developmental stages. The expression profiles of both anthocyanin biosynthetic genes and photorespiratory genes showed significant changes in leaves held in the dark or exposed to heat stress, compared with controls. The expression of anthocyanin biosynthetic genes dramatically decreased in peach red leaves following dark or heat treatments, resulting in a significant decrease of anthocyanin accumulation. However, the photorespiration-related genes GDCH and GOX exhibited increased expression in peach leaves after dark or heat treatment. Moreover, the expression levels of GDCH and GOX in the Arabidopsis chi/f3′h mutant that does not accumulate anthocyanins were higher than in the wild type. Overall, these results support the hypothesis that photorespiration-related genes might be involved in the regulation of anthocyanin biosynthesis. This finding provides a new insight into our understanding of the mechanism underlying the control of anthocyanin biosynthesis in plants. 相似文献
5.
Yasuhiro Shimizu Kazuhiro Maeda Mika Kato Koichiro Shimomura 《In vitro cellular & developmental biology. Plant》2010,46(5):460-465
Gynura bicolor DC., a traditional vegetable in Japan, is cultivated as Kinjisou and Suizenjina in Ishikawa and Kumamoto prefectures, respectively.
The adaxial side of the leaves of G. bicolor grown in a field is green, and the abaxial side is reddish purple. It has been reported that these reddish purple pigments
are anthocyanins. Although we established a culture system of G. bicolor, the leaves of G. bicolor plants grown under our culture conditions showed green color on both sides of all leaves. We investigated the effects of
phytohormones and chemical treatments on anthocyanin accumulation in cultured plants. Although anthocyanin accumulation in
the leaves was slightly stimulated, anthocyanins accumulation in the roots of the cultured plant was induced remarkably by
25–50 μM methyl jasmonate (MJ) treatment. This induction was affected by light irradiation and sucrose concentration in the
culture medium. However, salicylic acid (SA) and 1-aminocyclopropane-1-carboxylic acid did not induce anthocyanin accumulation
in roots. And then, combinations of MJ and SA or MJ and AgNO3 did not stimulate the anthocyanin accumulation in the root as found in the case of treatment by MJ solely. 相似文献
6.
7.
8.
9.
10.
11.
Prof. Carlo Cappelletti 《Plant biosystems》2013,147(2):436-457
Abstract Anthocyanins are secondary metabolites, which play important roles in the physiology of plants. In tomato (Solanum lycopersicum L.), anthocyanins are normally synthesized only in vegetative tissues. M375 is a mutant unable to produce anthocyanins in leaves and stems. In this study, we investigated the anthocyanin biosynthetic pathway in M375 and in its genetic background, Alice, in order to find out where the anthocyanin biosynthesis is blocked, along the pathway, in the mutant. Anthocyanins accumulation was enhanced by sucrose only in the wild type, even though the expression of several genes involved in anthocyanin biosynthesis was normal in both the genotypes. Genes coding for the final steps along the anthocyanin biosynthetic pathway were, however, less expressed in the M375 when compared to the wild type. 相似文献
12.
Marian Saniewski Marcin Horbowicz Jerzy Puchalski Junichi Ueda 《Acta Physiologiae Plantarum》2003,25(2):143-149
Methyl jasmonate (JA-Me) at concentrations of 0.1, 0.5 and 1.0 % (w/w) greatly stimulated anthocyanins accumulation in shoots
of young plants of Kalanchoe blossfeldiana when it was applied around the stem as a lanolin paste. Stimulatory effect of JA-Me was evidently observed as early as two
days after treatment. Anthocyanins were formed in the main and lateral shoots, including petioles, both below and above portions
of the treatment. When leaves were removed from the plant, almost no anthocyanin formation was observed. It should be mentioned
that leaves are necessary for the anthocyanin accumulation in stems induced by JA-Me. 相似文献
13.
14.
15.
Abscisic acid (ABA) at 3.8 µM suppressed both in vivoand in vitro nitrate reductase activity in roots, stems andleaves of potato plants grown in solution culture. Suppressionwas maximal between 24 and 48 h, followed by recovery of activityat 72 h in roots and leaves and at 96 h in stems. Removal from ABA after 24 h resulted in complete recovery ofnitrate reductase activity in roots by 24 h and partial recoveryin leaves. ABA treatment enhanced nitrate accumulation in roots,decreased that of leaves, but had no effect on stem nitratecontent. ABA enhanced decay of the enzyme following nitrate removal;by 7 h activity in roots was 22.5% of the initial value comparedto 55% in the control. ABA showed a less drastic effect on lossof activity in leaves and stems. These results indicate thatABA suppression of nitrate reductase activity is not dependenton nitrate uptake, and although it reduced leaf nitrate contentthere was no clear relationship between tissue nitrate levelsand the ABA response. (Received September 13, 1984; Accepted July 1, 1985) 相似文献
16.
17.
Abe Y Tera M Sasaki N Okamura M Umemoto N Momose M Kawahara N Kamakura H Goda Y Nagasawa K Ozeki Y 《Biochemical and biophysical research communications》2008,373(4):473-477
Carnations have anthocyanins acylated with malate. Although anthocyanin acyltransferases have been reported in several plant species, anthocyanin malyltransferase (AMalT) activity in carnation has not been identified. Here, an acyl donor substance of AMalT, 1-O-β-d-malylglucose, was extracted and partially purified from the petals of carnation. This was synthesized chemically to analyze AMalT activity in a crude extract from carnation. Changes in the AMalT activity showed close correlation to the accumulation of pelargonidin 3-malylglucoside (Pel 3-malGlc) during the development of red petals of carnation, but neither AMalT activity nor Pel 3-malGlc accumulation was detectable in roots, stems and leaves. 相似文献
18.
Not all anthocyanins are born equal: distinct patterns induced by stress in Arabidopsis 总被引:1,自引:0,他引:1
Nik Kovinich Gilbert Kayanja Alexandra Chanoca Ken Riedl Marisa S. Otegui Erich Grotewold 《Planta》2014,240(5):931-940
Main Conclusion
Different abiotic stress conditions induce distinct sets of anthocyanins, indicating that anthocyanins have different biological functions, or that decoration patterns of each anthocyanin are used for unique purposes during stress. The induction of anthocyanin accumulation in vegetative tissues is often considered to be a response of plants to biotic or abiotic stress conditions. Arabidopsis thaliana (Arabidopsis) accumulates over 20 anthocyanins derived from the anthocyanidin cyanidin in an organ-specific manner during development, but the anthocyanin chemical diversity for their alleged stress protective functions remains unclear. We show here that, when grown in various abiotic stress conditions, Arabidopsis not only often accumulates significantly higher levels of total anthocyanins, but different stress conditions also favor the accumulation of different sets of anthocyanins. For example, the anthocyanin patterns of seedlings grown at pH 3.3 or in media lacking phosphate are very similar and characterized by relatively high levels of the anthocyanins A8 and A11. In contrast, anthocyanin inductive conditions (AIC) provided by high sucrose media are characterized by high accumulation of A9* and A5 relative to other stress conditions. The modifications present in each condition correlate reasonably well with the induction of the respective anthocyanin modification enzymes. Taken together, our results suggest that Arabidopsis anthocyanin profiles provide ‘fingerprints’ that reflect the stress status of the plants. 相似文献19.
A Major Jasmonate-Inducible Protein of Sweet Potato, Ipomoelin, is an ABA-Independent Wound-Inducible Protein 总被引:3,自引:0,他引:3
Imanishi Shunsuke; Kito-Nakamura Kyoko; Matsuoka Ken; Morikami Atsushi; Nakamura Kenzo 《Plant & cell physiology》1997,38(6):643-652
Treatment of sweet potato plants cultured in vitro with a vaporof methyl jasmonate (MeJA) induced an accumulation in leavesof a large amount of protein with an apparent molecular massof 18 kDa. This protein, designated ipomoelin, was purified,and the amino acid sequences of proteolytic fragments were determined.Screening a cDNA library of MeJA-treated leaves by oligonucleotideprobes designed from the peptide sequences identified a clonethat could code for a polypeptide with 154 amino acids. Thededuced amino acid sequence of ipomoelin showed an overall aminoacid identity of 25% with the salt-inducible SalT protein ofrice. In addition, the C-terminal 70 amino acid sequence ofipomoelin showed about 50% identity with the C-terminal aminoacid sequences of seed lectins from Moraceae. The gene for ipomoelinwas present in a few copies in the genome of sweet potato. ThemRNA for ipomoelin was detected in leaves and petioles, butnot in stems and tuberous roots, of sweet potato plants grownin the field. Mechanical wounding of leaves induced ipomoelinmRNA both locally and systemically, while treatment of leaveswith ABA, salt, or a high level of sucrose did not induce ipomoelinmRNA. By contrast, ABA-inducible mRNA for sporamin was not inducedby MeJA. These results suggest that ipomoelin is involved indefensive reactions of leaves in response to wounding and thatJA-mediated wound-induction of ipomoelin occurs independentlyof ABA. (Received January 6, 1997; Accepted March 13, 1997) 相似文献
20.
Sweet potato NAC transcription factor,IbNAC1, upregulates sporamin gene expression by binding the SWRE motif against mechanical wounding and herbivore attack
下载免费PDF全文
![点击此处可从《The Plant journal : for cell and molecular biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Shi‐Peng Chen I Winnie Lin Xuanyang Chen Yin‐Hao Huang Shiao‐Chi Chang Hui‐Shan Lo Hseuh‐Han Lu Kai‐Wun Yeh 《The Plant journal : for cell and molecular biology》2016,86(3):234-248