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Inhibition by adenine derivatives of the cyanide-insensitive electron transport pathway of plant mitochondria 总被引:3,自引:2,他引:1
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The effect of benzylaminopurine was studied on cyanide-resistant mitochondria isolated from aged slices of potato tuber (Solanum tuberosum L. var. Bintje). Benzylaminopurine specifically acted on the cyanide-resistant alternative pathway. In the case of succinate oxidation, it mimicked the action of salicylhydroxamic acid and restored a good oxidative phosphorylation. Kinetic analyses showed that inhibitions by benzylaminopurine, salicylhydroxamic acid, and disulfiram occurred at mutually exclusive sites on the alternative pathway. Cyanide-resistant malate oxidation was only partially inhibited by benzylaminopurine and this inhibition occurred for low concentrations of this compound. On the other hand, the oxidation of exogenous NADH remained unaffected.
The effects of several adenine derivatives with or without cytokinin activity and that of a purine analog with anticytokinin activity were also studied. The variation in effectiveness to inhibit cyanide-resistant electron transport was: benzylaminopurine and 7-pentylamino-3-methylpyrazolo (4,3 d) pyrimidine (anticytokinin) > α-α′ dimethyl-allyl-adenine > 6-benzoylamino-9-benzylpurine > kinetin > adenine. No correlation was observed between the ability to inhibit the alternative pathway and the biological activity of these compounds. Liposolubility appeared as a major factor for potential inhibitory effect on the alternative pathway.
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Energy conservation associated with cyanide-insensitive respiration in plant mitochondria 总被引:12,自引:0,他引:12
S B Wilson 《Biochimica et biophysica acta》1970,223(2):383-387
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NADH oxidation and cytochrome c reduction rates in the electrontransport chain were determined for mitochondria isolated from leaves of matureEuropean black alder (Alnus glutinosa L.) and exposed to arange of concentrations of the growth retardants flurprimidol andpaclobutrazol.NADH oxidation and cytochrome c reduction were enhanced by low concentrationsofboth compounds whereas higher concentrations reduced electron transport. Thisisthe first report of gibberellin synthesis inhibitors affecting electrontransport in plant mitochondria and provides evidence for another potentialmodeof action of this type of growth retardant. 相似文献
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Trifluoperazine inhibition of electron transport and adenosine triphosphatase in plant mitochondria 总被引:4,自引:0,他引:4
P P Dunn A R Slabas I R Cottingham A L Moore 《Archives of biochemistry and biophysics》1984,229(1):287-294
Trifluoperazine inhibits ADP-stimulated respiration in mung bean (Phaseolus aureus) mitochondria when either NADH, malate, or succinate serve as substrates (IC50 values of 56, 59, and 55 microM, respectively). Succinate:ferricyanide oxidoreductase activity of these mitochondria was inhibited to a similar extent. The oxidation of ascorbate/TMPD was also sensitive to the phenothiazine (IC50 = 65 microM). Oxidation of exogenous NADH was inhibited by trifluoperazine even in the presence of excess EGTA [ethylene glycol bis(beta-aminoethyl ether)-N,N'-tetraacetic acid] (IC50 = 60 microM), indicating an interaction with the electron transport chain rather than with the dehydrogenase itself. In contrast, substrate oxidation in Voodoo lily (Sauromatum guttatum) mitochondria was relatively insensitive to the phenothiazine. The results suggest the bc1 complex to be a major site of inhibition. The membrane potential of energized mung bean mitochondria was depressed by micromolar concentrations of trifluoperazine, suggesting an effect on the proton-pumping capability of these mitochondria. Membrane-bound and soluble ATPases were equally sensitive to trifluoperazine (IC50 of 28 microM for both), implying the site of inhibition to be on the F1. Inhibition of the soluble ATPase was not affected by EGTA, CaCl2, or exogenous calmodulin. Trifluoperazine inhibition of electron transport and phosphorylation in plant mitochondria appears to be due to an interaction with a protein of the organelle that is not calmodulin. 相似文献
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The determination of the proton-motive force during cyanide-insensitive respiration in plant mitochondria 总被引:5,自引:0,他引:5
The magnitude of the components of the proton-motive force (Δp) generated in the presence of antimycin A has been determined for potato, mung bean, skunk cabbage, and Arum spadix mitochondria, Δp was calculated from the distribution of rubidium, methylamine, and 5,5′-dimethyl-2,4-oxazolodine-dione. In the presence of antimycin A, the oxidation of succinate generates a Δp of 40–50 mV, and this value is independent of the degree of antimycin A insensitivity of the various mitochondria. Under such conditions, the addition of ADP failed to either stimulate the respiratory rate or reduce Δp. Although oxygen consumption via the alternative pathway was sensitive to hydroxamic acids, no change in the components of the proton motive force was detected. The addition of an uncoupler in the presence of antimycin A and succinate reduced Δp to zero while respiration remained unaltered. The oxidation of malate in the presence of antimycin A generates a Δp of 150 mV, which was reduced to 144 mV under State 3 conditions. The addition of salicylhydroxamic acid inhibited oxygen uptake and reduced Δp to 40 mV. It is concluded that the oxidation of succinate by the alternative respiratory pathway does not generate a proton-motive force and is not coupled to ATP synthesis. The oxidation of malate by the alternative pathway, however, can conserve energy as ATP presumably via coupling Site I of the main respiratory chain. 相似文献
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Role of nonohmicity in the regulation of electron transport in plant mitochondria 总被引:2,自引:2,他引:0
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The relationship between the respiratory rate and the membrane ionic current on the protonmotive force has been investigated in percoll purified potato mitochondria. The dependence of the membrane ionic current on the membrane potential was monitored using a methyltriphenylphosphonium-sensitive electrode and determining the maximal net rate of depolarization following the addition of a respiratory inhibitor. We have confirmed that a nonohmic relationship exists between the ionic conductance and membrane potential. Addition of ATPase inhibitors markedly increased the initial rate of dissipation suggesting that in their absence the dissipation rate induced by respiratory inhibitors is partially offset by H+-efflux due to the hydrolysis of endogenous ATP. This was corroborated by direct measurement of endogenous ATP levels which decreased significantly following dissipation of the membrane potential. Results are discussed in terms of the regulation of electron transport in plant mitochondria in vivo. 相似文献
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O. I. Grabel’nykh A. V. Kolesnichenko T. P. Pobezhimova V. V. Zykova V. K. Voinikov 《Russian Journal of Plant Physiology》2006,53(3):418-429
Data are raeviewed on mitochondrial systems whose functioning in plants diminishes the efficiency of oxidative phosphorylation. The involvement in this process of alternative oxidase, thermogenin-like uncoupling proteins, a 310 kD stress protein, free fatty acids, and the ADP/ATP antiporter is considered. The role of these systems is discussed with regard to thermogenesis, controlled production of reactive oxygen species, and regulation of bioenergetics and metabolism. 相似文献
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Anthony L. Moore Paul E. Linnett R. Brian Beechey 《Journal of bioenergetics and biomembranes》1980,12(3-4):309-323
Dibutylchloromethyl tin chloride (DBCT) inhibits coupled and uncoupled respiration of mitochondria from potato tubers, cauliflower florets and etiolated mung bean hypocotyls with succinate andl-malate but not with external NADH or TMPD/ascorbate as substrates. Using potato and cauliflower mitochondria, DBCT at 200 pmole/mg of protein gives complete inhibition only in KCl-based media and at pH 6.8. DBCT has no effect on the internal pH of mung bean mitochondria, but does cause a decrease in the membrane potential. Electron transport through the alternative oxidase is not inhibited, neither is the ATP-synthase system. DBCT appears to interact with the functionally-distinct pool of ubiquinone associated with the oxidation of succinate andl-malate. 相似文献
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Kravets' VS 《Ukrainski? biokhimicheski? zhurnal》1999,71(5):23-28
The etiolated 2.5-day winter wheat sprouts were chilled at 3 degrees C during 24 to 144 hours. After 24 h cooling, shoot intact mitochondria showed a high degree of activation of the alternative oxidase, which was measured as sodium azide and benzohydroxamate sensitivity of the organelles respiration with succinate as a substrate. The role of the alternative oxidase in limiting the level of reactive oxygen species produced in the stressed plant tissues is discussed. 相似文献
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The multiplicity of dehydrogenases in the electron transport chain of plant mitochondria 总被引:2,自引:0,他引:2
The electron transport chain in mitochondria of different organisms contains a mixture of common and specialised components. The specialised enzymes form branches to the universal electron path, especially at the level of ubiquinone, and allow the chain to adjust to different cellular and metabolic requirements. In plants, specialised components have been known for a long time. However, recently, the known number of plant respiratory chain dehydrogenases has increased, including both components specific to plants and those with mammalian counterparts. This review will highlight the novel branches and their consequences for the understanding of electron transport and redundancy of electron paths. 相似文献
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Ca 2+ transport activity in mitochondria from some plant tissues 总被引:8,自引:0,他引:8
Mitochondria isolated from some 14 different higher plants and fungi were examined for their capacity to carry out respiration-dependent accumulation of Ca2+. Additions of Ca2+ give little or no stimulation of state 4 respiration of plant mitochondria, although the added Ca2+ was largely accumulated. Accumulation of Ca2+ required phosphate and, in most cases, was stimulated by Mg2+ and ADP or ATP. Ca2+ uptake was abolished by respiratory inhibitors and uncoupling agents. The ratio of Ca2+ ions taken up per pair of electrons per energy-conserving site was normal at about 2.0 for mitochondria from sweet potato and white potato; mitochondria from other plants showed somewhat lower ratios. Accumulated Ca2+ was only very slowly released from previously loaded plant mitochondria. Respiration-inhibited sweet potato mitochondria show both high-affinity and low-affinity Ca2+ binding sites sensitive to uncouplers, La3+, and ruthenium red and thus resemble animal mitochondria. Most other plant mitochondria lack high affinity sites. In general, mitochondria from sweet potato and white potato tubers resemble those from animal tissues, but mitochondria from carrots, beets, turnips, onions, cabbage, artichokes, cauliflower, avocados, mung bean and corn seedlings, and mushrooms show rather low affinity and activity in accumulation of Ca2+, probably due to lack of a specific Ca2+ carrier. 相似文献
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The properties of oxaloacetate (OA) transport into mitochondria from potato (Solanum tuberosum) tuber and pea (Pisum sativum) leaves were studied by measuring the uptake of 14C-labeled OA into liposomes with incorporated mitochondrial membrane proteins preloaded with various dicarboxylates or citrate. OA was found to be transported in an obligatory counterexchange with malate, 2-oxoglutarate, succinate, citrate, or aspartate. Phtalonate inhibited all of these countertransports. OA-malate countertransport was inhibited by 4,4′-dithiocyanostilbene-2,2′-disulfonate and pyridoxal phosphate, and also by p-chloromercuribenzene sulfonate and mersalyl, indicating that a lysine and a cysteine residue of the translocator protein are involved in the transport. From these and other inhibition studies, we concluded that plant mitochondria contain an OA translocator that differs from all other known mitochondrial translocators. Major functions of this translocator are the export of reducing equivalents from the mitochondria via the malate-OA shuttle and the export of citrate via the citrate-OA shuttle. In the cytosol, citrate can then be converted either into 2-oxoglutarate for use as a carbon skeleton for nitrate assimilation or into acetyl-coenzyme A for use as a precursor for fatty acid elongation or isoprenoid biosynthesis. 相似文献
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Comparison of the cyanide-resistant duroquinol oxidase activity of sub-mitochondrial particles from Arum maculatum L. with their ability to carry out a cyanide-resistant oxidation of NADH and succinate shows that heat-inactivation of the duroquinol oxidase activity does not proportionally affect NADH and succinate oxidation. Moreover, 1 microM antimycin inhibits duroquinol oxidase activity by 50% while not decreasing the rates of NADH and succinate oxidation. Therefore, the cyanide-resistant electron transport does not appear to be mediated by a "duroquinol oxidase", and a convincing proof of the existence of a specific protein acting as a cyanide-resistant oxidase in plant mitochondria is still lacking. 相似文献