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1.
T. R. Sharma P. Shanker B. K. Singh T. K. Jana M. S. Madhav K. Gaikwad N. K. Singh P. Plaha R. Rathour 《Journal of plant biochemistry and biotechnology.》2005,14(2):127-133
We have used rice line Tetep as a resistant donor with the aim of mapping a durable blast resistance gene Pi-k h using RAPD and AFLP techniques in conjunction with bulk segregant analysis. An F2 mapping population consisting of 205 plants was generated by crossing Tetep with HP2216, a highly susceptible cultivar. Inoculation with specific isolate (PLP-1) of Magnaporthe grisea at seeding stage showed that the Pi-k h gene inherited as a single dominant gene in F2 population. RAPD analysis was performed with 240 primers to detect polymorphism between resistant and susceptible parents. Of these, 48 primers produced polymorphic banding pattern between resistant and susceptible parents. Bulk segregant analysis was performed with 48 primers of which 5 showed polymorphism between resistant and susceptible bulks. A 700 bp DNA band was obtained in resistant F2 plants with primer 5-129 indicating its linkage to the resistance gene. Out of 64 AFLP primer combinations used for polymorphism survey between HP 2216 and Tetep, 11 AFLP primer combinations were able to distinguish the resistant and susceptible bulks. An AFLP band of 75 bp obtained with primer combination, E-TAlM-CTC co-segregated with the resistance gene. The RAPD marker 5-129700 and AFLP75 were placed on the linkage map at a distance of 2.1 eM and 15.1 eM flanking to Pi-k hgene, respectively. The RAPD band closely linked to Pi-k h gene was sequenced and used for the development of CAPs markers which also co-segregated with resistant phenotype in the mapping population. On sequence analysis and homology search of RAPD fragment with whole rice genome sequence database and the information available on physical, genetic and sequence maps of rice, the co-segregating CAPs marker was placed at long arm of rice chromosome 11. CAPs marker developed in this study showed polymorphism in different rice cultivars grown in North-Western Himalayan region and is being used for the pyramiding of Pi-k h gene along with other blast resistance genes using marker-assisted selection. 相似文献
2.
M. Srinivas Prasad B. Aruna Kanthi S. M. Balachandran M. Seshumadhav K. Madhan Mohan B. C. Viraktamath 《World journal of microbiology & biotechnology》2009,25(10):1765-1769
Samba mahsuri (BPT 5204) is a cultivar of the medium slender grain indica variety of Oryza
sativa grown across India for its high yield and quality. However, this cultivar is susceptible to several diseases and pests including
rice blast. The analysis of near isogenic lines indicated the presence of a resistance gene, Pi-1(t) in the donor cultivar C101LAC which is highly resistant to the rice blast fungus Magnaporthe grisea (M. grisea). C101LAC was crossed with susceptible indica rice cultivar (BPT 5204) to generate the mapping population. A mendelian segregation
ratio of 3:1 for resistant to susceptible F2 plants using bulk segregation analysis confirmed the presence of a major gene pi-1(t) by simple sequence repeats marker RM224 to the highly virulent blast isolate DRR 001. 相似文献
3.
Araújo L.G. Prabhu A.S. Filippi M.C. Chaves L.J. 《Plant Cell, Tissue and Organ Culture》2001,67(2):165-172
Seventeen somaclones of upland rice cultivar IAC 47 showing different plant types, and either resistance or susceptibility to leaf blast, were utilized for random amplified polymorphic DNA (RAPD) analysis. Somaclones exhibited differences in reaction to isolates of Pyricularia grisea. Two somaclones (SC02 and SC04) were resistant to all three field isolates of somaclones, while the cultivar IAC 47 was susceptible. The inheritance study of two distinct plant types, one with erect bright green leaves and the other with droopy yellow green leaves, showed that a single possibly different, dominant gene governs each plant type. Of 32 random decamer primers utilized, OPA02 and OPD02 detected polymorphisms between somaclones showing erect bright green leaves and droopy yellow green leaves. Reliable grouping exhibiting 80% similarity was achieved with 17 primers. Leaf blast resistance to race IC-2 of P. grisea was associated with the plant type of erect bright green leaves. 相似文献
4.
Kuraparthy Vasu Harjit Singh S. Singh Parveen Chhuneja H. S. Dhaliwal 《Journal of plant biochemistry and biotechnology.》2001,10(2):127-132
Triticum monococcum L, a diploid wheat species closely related to the A genome of cultivated wheats, is highly resistant to leaf rust. A synthetic amphiploid, T. monococcum — T. durum was crossed with T. aestivum cv WL711, highly susceptible to leaf rust. Leaf rust resistant derivatives were selected among backcross generations with the recurrent parent WL711 and cytologically analysed. Chromosome number of the leaf rust resistant BC1F3 progenies varied from 39 to 44. Six leaf rust resistant and susceptible bulks from different BC1F3 progenies were analysed using 29 wheat microsatellite(WMS) markers already mapped on A genome of bread wheat and found polymorphic among parents. One T. monococcum specific allele of WMS gwm136 locus was found to be closely linked to the leaf rust resistance gene in all the resistant bulks. Differential chromosome number, frequency of univalents and multivalents, however, indicated that the critical T. monococcum chromosome might be present in addition to the A genome chromosomes of wheat, substituted either for the B or D genome chromosome of wheat or translocated to chromosome 1A of wheat in one or the other bulks. The association of the T. monococcum specific allele of WMS gwm136 locus to leaf rust resistance was further confirmed from bulked segregant analysis in BC2F1 generation. 相似文献
5.
Sanju Kumari Jennifer M. Sheba Maheshwaran Marappan Shanmugasunderam Ponnuswamy Suresh Seetharaman Nagarajan Pothi Mohankumar Subbarayalu Raveendran Muthurajan Senthil Natesan 《Molecular biotechnology》2010,46(1):63-71
Brown planthopper (Nilaparvata lugens St?l) is one of the major insect pests of rice. A Sri Lankan indica rice cultivar Rathu Heenati was found to be resistant to all biotypes of the brown planthopper. In the present study, a total
of 268 F7 RILs of IR50 and Rathu Heenati were phenotyped for their level of resistance against BPH by the standard seedbox screening
test (SSST) in the greenhouse. A total of 53 SSR primers mapped on the chromosome 3 were used to screen the polymorphism between
the parents IR50 and Rathu Heenati, out of which eleven were found to be polymorphic between IR50 and Rathu Heenati. The eleven
primers that have shown polymorphism between the IR50 and Rathu Heenati parents were genotyped in a set of five resistant
RILs and five susceptible RILs along with the parents for co-segregation analysis. Among the eleven primers, two primers namely
RM3180 (18.22 Mb) and RM2453 (20.19 Mb) showed complete co-segregation with resistance. The identification of SSR markers
linked with BPH resistant could be used for the maker assisted selection (MAS) program in rice breeding and to map the resistant
genes on rice chromosomes for further gene cloning. 相似文献
6.
Molecular mapping of leaf rust resistance gene LrFun in Romanian wheat line Fundulea 900 总被引:1,自引:0,他引:1
Lifang Xing Cuifen Wang Xianchun Xia Zhonghu He Wanquan Chen Taiguo Liu Zaifeng Li Daqun Liu 《Molecular breeding : new strategies in plant improvement》2014,33(4):931-937
Leaf rust, caused by Puccinia triticina, is one of the major wheat diseases worldwide and poses a constant threat to common wheat (Triticum aestivum L.) production and food security. Results from the F2 and F2:3 populations derived from a cross between resistant line Fundulea 900 and susceptible cultivar Thatcher indicated that a single dominant gene, tentatively designated LrFun, conferred resistance to leaf rust. In order to identify other possible genes in Fundulea 900, nine P. triticina pathotypes avirulent on Fundulea 900 were used to inoculate F2:3 families. The results showed that at least two leaf rust resistance genes were present in Fundulea 900. A total of 1,706 pairs of simple sequence repeat (SSR) primers were used to test the parents and resistant and susceptible bulks. Eight polymorphic markers from chromosome 7BL were used for genotyping the F2 and F2:3 populations. LrFun was linked to eight SSR loci on chromosome 7BL. The two closest flanking SSR loci were Xgwm344 and Xwmc70, with genetic distances of 4.4 and 5.7 cM, respectively. At present four leaf rust resistance genes, Lr14a, Lr14b, Lr68 and LrBi16, are located on chromosome 7BL. In a seedling test with 12 P. triticina isolates, the reaction patterns of LrFun were different from those of lines carrying Lr14a, Lr14b and LrBi16. Lr68 is an adult plant resistance gene, and it is different from the seedling resistance gene LrFun. Therefore, we concluded that LrFun is a new leaf rust resistance gene. 相似文献
7.
Identification of a STS marker linked to the Aegilops speltoides-derived leaf rust resistance gene Lr28 in wheat 总被引:7,自引:0,他引:7
S. Naik K. S. Gill V. S. Prakasa Rao V. S. Gupta S. A. Tamhankar S. Pujar B. S. Gill P. K. Ranjekar 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(4):535-540
A sequence-tagged-site (STS) marker is reported linked to Lr28, a leaf rust resistance gene in wheat. RAPD (random amplified polymorphic DNA) analysis of near-isogenic lines (NILs) of
Lr28 in eight varietal backgrounds was carried out using random primers. Genomic DNA enriched for low-copy sequences was used
for RAPD analysis to overcome the lack of reproducibility due to the highly repetitive DNA sequences present in wheat. Of
80 random primers tested on the enriched DNA, one RAPD marker distinguished the NILs and the donor parent from the susceptible
recurrent parents. The additional band present in resistant lines was cloned, sequenced, and STS primers specific for Lr28 were designed. The STS marker (Indian patent pending: 380 Del98) was further confirmed by bulk segregation analysis of F3 families. It was consistently present in the NILs, the resistant F3 bulk and the resistant F3 lines, but was absent in recurrent parents, the susceptible F3 bulk and the susceptible F3 lines.
Received: 20 February 1998 / Accepted: 4 March 1998 相似文献
8.
Magnaporthe grisea, the blast fungus is one of the main pathological threats to finger millet crop worldwide. A systematic search for the blast
resistance gene analogs was carried out, using functional molecular markers. Three-fourths of the recognition-dependent disease
resistance genes (R-genes) identified in plants encodes nucleotide binding site (NBS) leucine-rich repeat (LRR) proteins. NBS-LRR homologs have
only been isolated on a limited scale from Eleusine coracana. Genomic DNA sequences sharing homology with NBS region of resistance gene analogs were isolated and characterized from resistant
genotypes of finger millet using PCR based approach with primers designed from conserved regions of NBS domain. Attempts were
made to identify molecular markers linked to the resistance gene and to differentiate the resistant bulk from the susceptible
bulk. A total of 9 NBS-LRR and 11 EST-SSR markers generated 75.6 and 73.5% polymorphism respectively amongst 73 finger millet
genotypes. NBS-5, NBS-9, NBS-3 and EST-SSR-04 markers showed a clear polymorphism which differentiated resistant genotypes
from susceptible genotypes. By comparing the banding pattern of different resistant and susceptible genotypes, five DNA amplifications
of NBS and EST-SSR primers (NBS-05504, NBS-09711, NBS-07688, NBS-03509 and EST-SSR-04241) were identified as markers for the blast resistance in resistant genotypes. Principal coordinate plot and UPGMA analysis
formed similar groups of the genotypes and placed most of the resistant genotypes together showing a high level of genetic
relatedness and the susceptible genotypes were placed in different groups on the basis of differential disease score. Our
results provided a clue for the cloning of finger millet blast resistance gene analogs which not only facilitate the process
of plant breeding but also molecular characterization of blast resistance gene analogs from Eleusine coracana. 相似文献
9.
F. SENTURK AKFIRAT F. ERTUGRUL S. HASANCEBI Y. AYDIN K. AKAN Z. MERT M. CAKIR A. ALTINKUT UNCUOGLU 《Journal of genetics》2013,92(2):233-240
We have previously reported Xgwm382 as a diagnostic marker for disease resistance against yellow rust in Izgi2001 × ES14 F2 population. Among the same earlier tested 230 primers, one SSR marker (Xgwm311) also amplified a fragment which is present in the resistant parent and in the resistant bulks, but absent in the susceptible parent and in the susceptible bulks. To understand the chromosome group location of these diagnostic markers, Xgwm382 and Xgwm311, in the same population, we selected 16 SSR markers mapped only in one genome of chromosome group 2 around 1–21 cM distance to these diagnostic markers based on the SSR consensus map of wheat. Out of 16 SSRs, Xwmc658 identified resistant F2 individuals as a diagnostic marker for yellow rust disease and provided the location of Xgwm382 and Xgwm311 on chromosome 2AL in our plant material. 相似文献
10.
PCR-based DNA markers linked to a gall midge resistance gene, Gm4t, has potential for marker-aided selection in rice 总被引:3,自引:0,他引:3
S. Nair A. Kumar M. N. Srivastava M. Mohan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(6):660-665
Rice DNAs from a gall midge resistant variety, Abhaya, a susceptible variety, Tulsi and their F3 progeny were screened using 500 random primers in conjunction with bulked-segregant analysis in a polymerase chain reaction (PCR) with a view to detecting random amplified polymorphic DNAs (RAPDs) linked to the gene, Gm4t, which confers resistance to gall midge, a dipteran insect pest of rice. A total of 454 primers were able to produce a distinct amplification pattern, and 3695 bands/loci were amplified between the phenotypically different parents. Of these, 304 bands were polymorphic between the parents, with 19 being phenotypespecific. One of these primers, E20, amplified 2 bands, E20570 and E20583, which are tightly linked to resistance and susceptibility, respectively. These specific bands were cloned and sequenced, and a 94% sequence homology was found between the two fragments. Two specific 20-mer oligonucleotides were synthesized, based on the sequence information of E20583, for use in PCR amplification directly from genomic DNAs. These PCR primers were able to amplify phenotype-specific bands, a 583-bp fragment in susceptible F3 lines and a 570-bp fragment in resistant F3 lines that had been derived from a cross between the parents, indicating their potential and utility for marker-aided selection of the Gm4t gene in rice. Its use would facilitate the early and efficient selection of resistant genes in plant breeding programmes and even in those areas where the insect is not known to occur. These phenotype-specific bands are single-copy sequences and are being mapped to ascertain their chromosomal location in rice. 相似文献
11.
Chen S Liu X Zeng L Ouyang D Yang J Zhu X 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(7):1331-1338
A new bacterial blight recessive resistance gene xa34(t) was identified from the descendant of somatic hybridization between an aus rice cultivar (cv.) BG1222 and susceptible cv. IR24 against Chinese race V (isolate 5226). The isolate was used to test the resistance or susceptibility of F1 progenies and reciprocal crosses of the parents. The results showed that F1 progenies appeared susceptibility there were 128R (resistant):378S (susceptible) and 119R:375S plants in F2 populations derived from two crosses of BG1222/IR24 and IR24/BG1222, respectively, which both calculates into a 1R:3S ratio. 320 pairs of stochastically selected SSR primers were used for genes?? initial mapping. The screened results showed that two SSR markers, RM493 and RM446, found on rice chromosome 1 linked to xa34(t). Linkage analysis showed that these two markers were on both sides of xa34(t) with the genetic distances 4.29 and 3.05?cM, respectively. The other 50 SSR markers in this region were used for genes?? fine mapping. The further results indicated that xa34(t) was mapped to a 1.42?cM genetic region between RM10927 and RM10591. In order to further narrow down the genomic region of xa34(t), 43 of insertion/deletion (Indel) markers (BGID1-43) were designed according to the sequences comparison between japonica and indica rice. Parents?? polymorphic detection and linkage assay showed that the Indel marker BGID25 came closer to the target gene with a 0.4?cM genetic distance. A contig map corresponding to the locus was constructed based on the reference sequences aligned by the xa34(t) linked markers. Consequently, the locus of xa34(t) was defined to a 204?kb interval flanked by markers RM10929 and BGID25. 相似文献
12.
Molecular mapping of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26 总被引:15,自引:0,他引:15
Li GQ Li ZF Yang WY Zhang Y He ZH Xu SC Singh RP Qu YY Xia XC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(8):1434-1440
Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most devastating diseases in common wheat (Triticum aestivum L.) worldwide. The objectives of this study were to map a stripe rust resistance gene in Chinese wheat cultivar Chuanmai 42 using molecular markers and to investigate its allelism with Yr24 and Yr26. A total of 787 F2 plants and 186 F3 lines derived from a cross between resistant cultivar Chuanmai 42 and susceptible line Taichung 29 were used for resistance gene tagging. Also 197 F2 plants from the cross Chuanmai 42×Yr24/3*Avocet S and 726 F2 plants from Chuanmai 42×Yr26/3*Avocet S were employed for allelic test of the resistance genes. In all, 819 pairs of wheat SSR primers were used to test the two parents, as well as resistant and susceptible bulks. Subsequently, nine polymorphic markers were employed for genotyping the F2 and F3 populations. Results indicated that the stripe rust resistance in Chuanmai 42 was conferred by a single dominant gene, temporarily designated YrCH42, located close to the centromere of chromosome 1B and flanked by nine SSR markers Xwmc626, Xgwm273, Xgwm11, Xgwm18, Xbarc137, Xbarc187, Xgwm498, Xbarc240 and Xwmc216. The resistance gene was closely linked to Xgwm498 and Xbarc187 with genetic distances of 1.6 and 2.3 cM, respectively. The seedling tests with 26 PST isolates and allelic tests indicated that YrCH42, Yr24 and Yr26 are likely to be the same gene.G.Q. Li and Z.F. Li contributed equally to the work. 相似文献
13.
A major locus for submergence tolerance mapped on rice chromosome 9 总被引:18,自引:0,他引:18
Kenong Xu David J. Mackill 《Molecular breeding : new strategies in plant improvement》1996,2(3):219-224
Submergence stress is a widespread problem in rice-growing environments where drainage is impeded. A few cultivars can tolerate more than 10 days of submergence, but the genes conferring this tolerance have not been identified. We used randon-amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers to map submergence tolerance in 169 F2 plants and the resulting F3 families of a cross between a tolerant indica rice line, IR40931-26, and a susceptible japonica line, PI543851. IR40931-26 inherited strong submergence tolerance from the unimproved cultivar FR13A. Eight-day old F3 seedlings were submerged for 14–16 days in 55-cm deep tanks, and tolerance was scored after 7 days recovery on a scale of 1 (tolerant) to 9 (susceptible). The tolerant and susceptible parents scored 1.5 and 8.4, respectively, and the F3 means ranged from 1.6 to 8.9. Two bulks were formed with DNA from F2 plants corresponding to the nine most tolerant and the nine most susceptible F3 families. Of 624 RAPD primers used to screen the bulks, five produced bands associated with either tolerance or susceptibility. These markers were mapped to a region of chromosome 9 by linkage to RFLP markers. A submergence tolerance quantitative trait locus (QTL), here designatedSub1, was located ca. 4 cM from the RFLP marker C1232 and accounted for 69% of the phenotypic variance for the trait. 相似文献
14.
Blast disease caused by the pathogen Pyricularia oryzae is a serious threat to rice production. Six generations viz., P1, P2, F1, F2, B1 and B2 of a cross between blast susceptible high-yielding rice cultivar ADT 43 and resistant near isogenic line (NIL) CT13432-3R, carrying four blast resistance genes Pi1, Pi2, Pi33 and Pi54 in combination were used to study the nature and magnitude of gene action for disease resistance and yield attributes. The epistatic interaction model was found adequate to explain the gene action in most of the traits. The interaction was complementary for number of productive tillers, economic yield, lesion number, infected leaf area and potential disease incidence but duplicate epistasis was observed for the remaining traits. Among the genotypes tested under epiphytotic conditions, gene pyramided lines were highly resistant to blast compared to individuals with single genes indicating that the nonallelic genes have a complementary effect when present together. The information on genetics of various contributing traits of resistance will further aid plant breeders in choosing appropriate breeding strategy for blast resistance and yield enhancement in rice. 相似文献
15.
A molecular marker that segregates with sorghum leaf blight resistance in one cross is maternally inherited in another 总被引:4,自引:0,他引:4
Leaf blight-resistant sorghum accession SC326-6 was crossed to the susceptible cultivar BTx623 to analyze the genetic basis
for resistance. Field scoring of inoculated F2 progeny revealed that resistance was transmitted as a dominant single-gene trait. By combining the random amplified polymorphic
DNA (RAPD) technique with bulked-segregant analysis, it was possible to identify PCR amplification products that␣segregated
with disease response. Primer OPD12 amplified a 323-bp band (D12R) that segregated with resistance. Creation of longer primers,
or SCARs (sequence characterized amplified regions) for D12R resulted in the amplification of a single major band of the predicted
size from all the resistant F2 progeny and the resistant parent SC326-6, but not from BTx623 or 24 of 29 susceptible F2 progeny. The SCAR primers also amplified a single band with DNA from IS3620C, the female parent in a cross with BTx623 that
has been used to produce a recombinant inbred population for RFLP mapping. An equivalent band was amplified from all 137 recombinant
inbred progeny, indicating that organelle DNA is the amplification target in this cross.
Received: 31 July 1998 / Accepted: 23 November 1998 相似文献
16.
QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice 总被引:14,自引:0,他引:14
S. Fukuoka K. Okuno 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(2-3):185-190
Field resistance is defined as the resistance that allows effective control of a parasite under natural field conditions and
is durable when exposed to new races of that parasite. To identify the genes for field resistance to rice blast, quantitative
trait loci (QTLs) conferring field resistance to rice blast in Japanese upland rice were detected and mapped using RFLP and
SSR markers. QTL analysis was carried out in F4 progeny lines from the cross between Nipponbare (moderately susceptible, lowland) and Owarihatamochi (resistant, upland).
Two QTLs were detected on chromosome 4 and one QTL was detected on each of chromosomes 9 and 12. The phenotypic variation
explained by each QTL ranged from 7.9 to 45.7% and the four QTLs explained 66.3% of the total phenotypic variation. Backcrossed
progeny lines were developed to transfer the QTL with largest effect using the susceptible cultivar Aichiasahi as a recurrent
parent. Among 82 F3 lines derived from the backcross, resistance segregated in the expected ratio of resistant 1 : heterozygous 2 : susceptible
1. The average score for blast resistance measured in the field was 4.2 ± 0.67, 7.5 ± 0.51and 8.2 ± 0.66, for resistant, heterozygous
and susceptible groups, respectively. The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively. The relationship to previously reported major genes and QTLs conferring
resistance to blasts, and the significance of marker-assisted selection to improve field resistance, are discussed.
Received: 8 June 2000 / Accepted: 24 November 2000 相似文献
17.
Singh M Chaudhary K Boora KS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,114(1):187-192
Anthracnose, caused by Colletotrichum graminicola, infects all aerial parts of sorghum, Sorghum bicolor (L.) Moench, plants and causes loss of as much as 70%. F1 and F2 plants inoculated with local isolates of C. graminicola indicated that resistance to anthracnose in sorghum accession G 73 segregated as a recessive trait in a cross with susceptible cultivar HC 136. To facilitate the use of marker-assisted selection in sorghum breeding programs, a PCR-based specific sequence characterized amplified region (SCAR) marker was developed. A total of 29 resistant and 20 susceptible recombinant inbred lines (RILs) derived from a HC 136 × G 73 cross was used for bulked segregant analysis to identify a RAPD marker closely linked to a gene for resistance to anthracnose. The polymorphism between the parents HC 136 and G 73 was evaluated using 84 random sequence decamer primers. Among these, only 24 primers generated polymorphism. On bulked segregant analysis, primer OPA 12 amplified a unique band of 383 bp only in the resistant parent G 73 and resistant bulk. Segregation analysis of individual RILs showed the marker OPA 12383 was 6.03 cM from the locus governing resistance to anthracnose. The marker OPA 12383 was cloned and sequenced. Based on the sequence of cloned RAPD product, a pair of SCAR markers SCA 12-1 and SCA 12-2 was designed using the MacVector program, which specifically amplified this RAPD fragment in resistant parent G 73, resistant bulk and respective RILs. Therefore, it was confirmed that SCAR marker SCA 12 is at the same locus as RAPD marker OPA 12383 and hence, is linked to the gene for resistance to anthracnose. 相似文献
18.
Stable resistance to infection with Wheat streak mosaic virus (WSMV) can be evolved de novo in selfing bread wheat lines subjected to cycles of WSMV inoculation and selection of best-performing plants or tillers. To learn whether this phenomenon might be applied to evolve resistance de novo to pathogens unrelated to WSMV, we examined the responses to leaf rust of succeeding generations of the rust- and WSMV-susceptible cultivar ‘Lakin’ following WSMV inoculation and derived rust-resistant sublines. After three cycles of the iterative protocol five plants, in contrast to all others, expressed resistance to leaf and stripe rust. A subset of descendant sublines of one of these, ‘R1’, heritably and uniformly expressed the new trait of resistance to leaf rust. Such sublines, into which no genes from a known source of resistance had been introgressed, conferred resistance to progeny of crosses with susceptible parents. The F1 populations produced from crosses between, respectively, susceptible and resistant ‘Lakin’ sublines 4-3-3 and 4-12-3 were not all uniform in their response to seedling inoculation with race TDBG. In seedling tests against TDBG and MKPS races the F2s from F1 populations that were uniformly resistant had 3∶1 ratios of resistant to susceptible individuals but the F2s from susceptible F1 progenitors were uniformly susceptible. True-breeding lines derived from resistant individuals in F2 populations were resistant to natural stripe and leaf rust inoculum in the field, while the ‘Lakin’ progenitor was susceptible. The next generation of six of the ‘Lakin’-derived lines exhibited moderate to strong de novo resistance to stem rust races TPMK, QFCS and RKQQ in seedling tests while the ‘Lakin’ progenitor was susceptible. These apparently epigenetic effects in response to virus infection may help researchers fashion a new tool that expands the range of genetic resources already available in adapted germplasm. 相似文献
19.
W. G. Xu C. X. Li L. Hu L. Zhang J. Z. Zhang H. B. Dong G. S. Wang 《Molecular breeding : new strategies in plant improvement》2010,26(1):31-38
The Chinese winter wheat cultivar Zhoumai 22 is highly resistant to powdery mildew. The objectives of this study were to map
a powdery mildew resistance gene in Zhoumai 22 using molecular markers and investigate its allelism with Pm13. A total of 278 F2 and 30 BC1 plants, and 143 F3 lines derived from the cross between resistant cultivar Zhoumai 22 and susceptible cultivar Chinese Spring were used for
resistance gene tagging. The 137 F2 plants from the cross Zhoumai 22/2761-5 (Pm13) were employed for the allelic test of the resistance genes. Two hundred and ten simple sequence repeat (SSR) markers were
used to test the two parents, and resistant and susceptible bulks. Subsequently, seven polymorphic markers were used for genotyping
the F2 and F3 populations. The results indicated that the powdery mildew resistance in Zhoumai 22 was conferred by a single dominant gene,
designated PmHNK tentatively, flanked by seven SSR markers Xgwm299, Xgwm108, Xbarc77, Xbarc84, Xwmc326, Xwmc291 and Xwmc687 on chromosome 3BL. The resistance gene was closely linked to Xwmc291 and Xgwm108, with genetic distances of 3.8 and 10.3 cM, respectively, and located on the chromosome bin 3BL-7-0.63-1.0 in the test with
a set of deletion lines. Seedling tests with seven isolates of Blumeria
graminis f. sp. tritici (Bgt) and allellic test indicated that PmHNK is different from Pm13, and Pm41 seems also to be different from PmHNK due to its origin from T. dicoccoides and molecular evidence. These results indicate that PmHNK is likely to be a novel powdery mildew resistance gene in wheat. 相似文献
20.
Hai Zhang Xianchun Xia Zhonghu He Xing Li Zaifeng Li Daqun Liu 《Molecular breeding : new strategies in plant improvement》2011,28(4):527-534
Leaf rust, caused by Puccinia triticina, is one of the most widespread diseases in common wheat (Triticum aestivum L.) globally. With the objective of identifying and mapping new genes for resistance to leaf rust, F1, F2 plants and F3 lines from a cross between resistant cultivar Bimai 16 and susceptible cultivar Thatcher were inoculated with Chinese Puccinia triticina pathotypes FHTT and PHTS in the greenhouse. In the first seedling test, Bimai 16, Thatcher, 20 F1 plants, 359 F2 plants and 298 F3 lines were inoculated with pathotype FHTT. A set of 1,255 simple sequence repeat (SSR) primer pairs were used to test the
parents, and resistant and susceptible bulks. Seven polymorphic markers on chromosome 7BL were used for genotyping the F2 and F3 populations. The results indicated that Bimai 16 carried a single dominant resistance gene, temporarily designated LrBi16, closely linked to SSR markers Xcfa2257 and Xgwm344, with genetic distances of 2.8 and 2.9 cM, respectively. In the second seedling test, two dominant resistance genes were
identified in Bimai 16 based on seedling reactions of 254 F2 plants inoculated with pathotype PHTS. One of the genes was LrBi16, and the other was likely to be LrZH84, which is located in chromosome 1BL. The seedling reaction pattern of plants with LrBi16 was different from that of the Thatcher lines, with Lr14a and Lr14b located on chromosome 7BL. It was concluded that LrBi16 is likely to be a new leaf rust resistance gene. 相似文献