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Sex determination 总被引:1,自引:0,他引:1
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Quantitative determination of prostaglandins 总被引:2,自引:0,他引:2
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MYSZKOWSKI L 《Acta physiologica Polonica》1957,8(3-3A):476-478
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VISWANATHAN R 《The Biochemical journal》1951,48(2):239-240
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The determination of hydroxyproline 总被引:111,自引:0,他引:111
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Quantitative determination of methylindoles 总被引:2,自引:0,他引:2
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Fluorometric methods are described for the determination of the enzymes hexokinase and β-glucosidase, and the carbohydrates glucose, fructose, maltose, cellobiose, lactose, glycogen, and salicin Glucose and fructose are determined fluorometrically using hexokinase and the resazurin-resorufin indicator reaction. Maltose, cellobiose, lactose, glycogen, and salicin are enzymically hydrolyzed to glucose, which is then determined fluorometrically using glucose oxidase, p-hydroxyphenylacetic acid, and peroxidase. All the carbohydrates were assayed in the range 0.01–50 μg/ml and the enzymes hexokinase and β-glucosidase in the range 10?3 to 10?1 unit/ml with an accuracy and precision of about 1.5%. 相似文献