共查询到20条相似文献,搜索用时 15 毫秒
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Sequences within the spacer region of yeast rRNA cistrons that stimulate 35S rRNA synthesis in vivo mediate RNA polymerase I-dependent promoter and terminator activities. 总被引:13,自引:8,他引:5
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R Mestel M Yip J P Holland E Wang J Kang M J Holland 《Molecular and cellular biology》1989,9(3):1243-1254
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S J Sulakhe 《FEBS letters》1986,204(2):302-306
gamma-Glutamyltranspeptidase is expressed at low levels in the liver of the male Fischer 344 rat where it exhibits 15-fold purification and 33% recovery in isolated plasma membranes. While the activity of the enzyme is unaltered in regenerating liver 24 h after partial hepatectomy, it increases steadily thereafter over a period of one week. Seven days after partial hepatectomy the enzyme is maximally activated: 5.6-fold in liver homogenates and 5.3-fold in isolated liver plasma membranes. The enzyme declines in activity over the next fourteen days and is expressed at normal levels three weeks after partial hepatectomy. These results demonstrate that the activity of gamma-glutamyltranspeptidase increases in regenerating liver but that the increase is out of phase with the proliferative response. 相似文献
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Messenger RNA in regenerating liver: implications for the understanding of regulated growth 总被引:1,自引:0,他引:1
Nelson Fausto 《Molecular and cellular biochemistry》1984,59(1-2):131-147
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B G Nair L Steinke Y M Yu H M Rashed J M Seyer T B Patel 《The Journal of biological chemistry》1991,266(1):567-573
Forty-eight hours after partial (approximately 67%) hepatectomy the activity of the particulate guanylate cyclase was increased by 2-fold in the regenerating rat liver. This increase was not an artifact of membrane isolation procedures, and as determined by 125I-labeled Tyr-28 atrial natriuretic hormone-(1-28) ANF binding, was accompanied by a 2-fold increase in the number of ANF receptors. The Kd of the receptors in membranes of regenerating livers was not significantly different from the Kd of the receptors in livers of sham-operated rats. The linear synthetic descysteine analog of ANF, analog I, which binds only to the 66-kDa receptors, displaced approximately 40% of the specifically bound 125I-ANF in liver membranes from both hepatectomized and sham-operated (control) animals. Affinity cross-linking studies with 125I-ANF confirmed the increase in the 116-kDa ANF receptor in membranes of regenerating livers. In perfused livers derived from control and hepatectomized animals, the basal rates of cGMP production were not significantly different. However, atriopeptin II-stimulated cGMP production was twice as great in regenerating livers as compared with controls. These data demonstrate that the increase in particulate guanylate cyclase activity observed during liver regeneration is due to an increase in the 116-kDa ANF receptor-associated activity. Additionally, our data demonstrate that the regenerating rat liver may be a valuable model with which to study the role of the hepatic ANF receptor/particulate guanylate cyclase. 相似文献
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