共查询到20条相似文献,搜索用时 15 毫秒
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c-Jun/激活蛋白-1活性调节研究进展 总被引:3,自引:0,他引:3
转录因子激活蛋白-1(AP-1)对细胞增殖、细胞存活与细胞凋亡等重要生理过程具有调控作用,其核心组成成分是c-Jun.c-Jun活性从转录调控、翻译后调控(主要是磷酸化调节)和相互作用蛋白质调节等三个水平受到正负向调控.其分子内8个位点可被JNK1、GSK3、CKII、Abl等激酶磷酸化.通过N端的转录激活结构域和C端的碱性亮氨酸拉链区,c-Jun可与bZIP类转录因子、辅助激活因子和其他一些蛋白质直接相互作用而被调控.另外一些分子可通过CBP、JAB1等重要辅助激活因子的介导间接调控AP-1的活性,共同构成AP-1活性调节的复杂网络. 相似文献
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Insulin enhances the expression of the endothelial nitric oxide synthase in native endothelial cells: a dual role for Akt and AP-1 总被引:2,自引:0,他引:2
Insulin-induced vasodilatation in vivo has been attributed to the activation of the endothelial nitric oxide (NO) synthase (eNOS). The present study addressed the effects of insulin on the activity and expression of eNOS in native and cultured endothelial cells. Insulin applied to native porcine aortic endothelial cells elicited the tyrosine phosphorylation of the insulin receptor and receptor substrate, the subsequent activation of phosphatidylinositol 3-kinase (PI 3-K), Akt (protein kinase B), and ERK1/2. Insulin did not activate eNOS in cultured endothelial cells nor relax endothelium-intact arterial segments. However, 4h after application of insulin to native endothelial cells eNOS mRNA was increased 2-fold. A comparable increase in eNOS protein was detected after 18-24h and associated with an increase in intracellular cyclic GMP. In native endothelial cells, insulin enhanced the DNA-binding activity of Sp1 and AP-1, but not that of NF-kappaB. The insulin-induced increase in eNOS expression was prevented by wortmannin as well as by AP-1 decoy oligonucleotides. The MEK1 inhibitor, PD 98059, also enhanced eNOS expression in native and cultured endothelial cells, an effect which was independent of ERK1/2 and associated with an increase in the DNA-binding activity of AP-1 and Sp1. These results demonstrate that insulin activates multiple signalling pathways in endothelial cells but does not acutely activate eNOS. Insulin however enhances eNOS mRNA and protein by a mechanism involving the combined activation of a PI 3-K- and AP-1-dependent pathway. 相似文献
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Wiejak J Dunlop J Stoyle C Lappin G McIlroy A Pediani JD Gao S Yarwood SJ 《Cellular signalling》2012,24(8):1690-1699
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JNK and AP-1 mediate apoptosis induced by bortezomib in HepG2 cells via FasL/caspase-8 and mitochondria-dependent pathways 总被引:3,自引:0,他引:3
Lauricella M Emanuele S D'Anneo A Calvaruso G Vassallo B Carlisi D Portanova P Vento R Tesoriere G 《Apoptosis : an international journal on programmed cell death》2006,11(4):607-625
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