共查询到20条相似文献,搜索用时 546 毫秒
1.
A. Setiawan G. Koch S. R. Barnes C. Jung 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(8):1176-1182
The breeding of sugar beet varieties that combine resistance to Cercospora and high yield under non-diseased conditions is a major challenge to the breeder. The understanding of the quantitative trait
loci (QTLs) contributing to Cercospora resistance offers one route to solving this problem. A QTL analysis of Cercospora resistance in sugar beet was carried out using a linkage map based on AFLP and RFLP markers. Two different screening methods
for Cercospora resistance (a field test at Copparo, Italy, under natural infection, and a newly-developed leaf disc test) were used to estimate
the level of Cercospora resistance; the correlation between scores from the field (at 162 days after sowing) and the leaf disc test was significant.
QTL analysis was based on F2 and F3 (half-sib family) generations derived from crosses between diploid single plants of 93164P (resistant to Cercospora leaf spot disease) and 95098P (susceptible). Four QTLs associated with Cercospora resistance (based on Lsmean data of the leaf disc test) on chromosomes III, IV, VII and IX were revealed using Composite
interval mapping. To produce populations segregating for leaf spot resistance as a single Mendelian factor, we selected for
plants heterozygous for only one of the QTLs (on chromosome IV or IX) but homozygous for the others.
Received: 1 September 1999 / Accepted 7 October 1999 相似文献
2.
Taguchi K Ogata N Kubo T Kawasaki S Mikami T 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(2):227-234
Aphanomyces root rot, caused by Aphanomyces cochlioides Drechs., is one of the most serious diseases of sugar beet (Beta vulgaris L.). Identification and characterization of resistance genes is a major task in sugar beet breeding. To ensure the effectiveness
of marker-assisted screening for Aphanomyces root rot resistance, genetic analysis of mature plants’ phenotypic and molecular
markers’ segregation was carried out. At a highly infested field site, some 187 F2 and 66 F3 individuals, derived from a cross between lines ‘NK-310mm-O’ (highly resistant) and ‘NK-184mm-O’ (susceptible), were tested,
over two seasons, for their level of resistance to Aphanomyces root rot. This resistance was classified into six categories
according to the extent and intensity of whole plant symptoms. Simultaneously, two selected RAPD and 159 ‘NK-310mm-O’-coupled
AFLP were used in the construction of a linkage map of 695.7 cM. Each of nine resultant linkage groups was successfully anchored
to one of nine sugar beet chromosomes by incorporating 16 STS markers. Combining data for phenotype and molecular marker segregation,
a single QTL was identified on chromosome III. This QTL explained 20% of the variance in F2 population (in the year 2002) and 65% in F3 lines (2003), indicating that this QTL plays a major role in the Aphanomyces root rot resistance. This is the first report
of the genetic mapping of resistance to Aphanomyces-caused diseases in sugar beet. 相似文献
3.
A genetic map of Maritime pine based on AFLP, RAPD and protein markers 总被引:12,自引:0,他引:12
P. Costa D. Pot C. Dubos J. M. Frigerio C. Pionneau C. Bodenes E. Bertocchi M. -T. Cervera D. L. Remington C. Plomion 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(1):39-48
TheAFLP (amplified fragment length polymorphism) technique was adapted to carry out genetic analysis in maritime pine, a species
characterized by a large genome size (24 pg/C). A genetic linkage map was constructed for one F1 individual based on 239 AFLP and 127 RAPD (randomly amplified polymorphic DNA) markers. Markers were scored on megagametophytes
(1n) from 200 germinated F2 seedlings. Polymorphism rate, labour time and cost of both AFLP and RAPD techniques were compared. The AFLP technique was
found to be twice as fast and three-times less costly per marker than the RAPD technique. Thirteen linkage groups were identified
with a LOD score ≥6 covering 1873 cM, which provided 93.4% of genome coverage. Proteins were extracted from needles (2n) of
the F2 progeny and revealed by 2-DE (two-dimensional electrophoresis). Thirty one segregating proteins were mapped using a QTL detection
strategy based on the quantification of protein accumulation. Two framework maps of the same F1 individual are now available. The first map (Plomion et al. 1996) uses RAPD markers and the second map, presented in this
study, uses mostly AFLP markers. Although the total genetic length of both maps was almost identical, differences among homologous
groups were observed.
Received: 11 February 1999 / Accepted: 29 April 1999 相似文献
4.
K. Pillen G. Steinrücken G. Wricke R. G. Herrmann C. Jung 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,84(1-2):129-135
Summary We have established a first linkage map for beets based on RFLP, isozyme and morphological markers. The population studied consisted of 96 F2 individuals derived from an intraspecific cross. As was expected for outbreeding species, a relatively high degree of polymorphism was found within sugar beet; 47% of the DNA markers were polymorphic for the chosen population. The map consists of 115 independent chromosomal loci designated by 108 genomic DNA probes, 6 isozyme and one morphological marker. The loci cover 789 cM with an average spacing of 6.9 cM. They are dispersed over nine linkage groups corresponding to the haploid chromosome number of Beta species. Eighteen markers (15.4%) showed distorted segregation which, in most instances, can be explained by gametic selection of linked lethal loci. The application of the linkage map in sugar beet breeding is discussed. 相似文献
5.
R. Heller J. Schondelmaier G. Steinrücken C. Jung 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,92(8):991-997
Sugar beet (Beta vulgaris L.) is highly susceptible to the beet cyst nematode (Heterodera schachtii Schm.). Three resistance genes originating from the wild beets B. procumbens (Hs1
pro-1) and B. webbiana (Hs1
web-1, Hs2
web-7) have been transferred to sugar beet via species hybridization. We describe the genetic localization of the nematode resistance genes in four different sugar beet lines using segregating F2 populations and RFLP markers from our current sugar beet linkage map. The mapping studies yielded a surprising result. Although the four parental lines carrying the wild beet translocations were not related to each other, the four genes mapped to the same locus in sugar beet independent of the original translocation event. Close linkage (0–4.6 cM) was found with marker loci at one end of linkage group IV. In two populations, RFLP loci showed segregation distortion due to gametic selection. For the first time, the non-randomness of the translocation process promoting gene transfer from the wild beet to the sugar beet is demonstrated. The data suggest that the resistance genes were incorporated into the sugar beet chromosomes by non-allelic homologous recombination. The finding that the different resistance genes are allelic will have major implications on future attempts to breed sugar beet combining the different resistance genes. 相似文献
6.
Pi35(t), a new gene conferring partial resistance to leaf blast in the rice cultivar Hokkai 188 总被引:1,自引:0,他引:1
Nguyen TT Koizumi S La TN Zenbayashi KS Ashizawa T Yasuda N Imazaki I Miyasaka A 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(4):697-704
The japonica rice cultivar Hokkai 188 shows a high level of partial resistance to leaf blast. For mapping genes conferring the resistance, a set of 190 F2 progeny/F3 families was developed from the cross between the indica rice cultivar Danghang-Shali, with a low level of partial resistance, and Hokkai 188. Partial resistance to leaf blast in the F3 families was assessed in upland nurseries. From a primary microsatellite (SSR) linkage map and QTL analysis using a subset of 126 F2 progeny/F3 families randomly selected from the above set, one major QTL located on chromosome 1 was detected in the vicinity of SSR marker RM1216. This QTL was responsible for 69.4% of the phenotypic variation, and Hokkai 188 contributed the resistance allele. Segregation analysis in the F3 families for partial resistance to leaf blast was in agreement with the existence of a major gene, and the gene was designated as Pi35(t). Another QTL detected on chromosome 8 was minor, explained 13.4% of the phenotypic variation, and an allele of Danghang-Shali increased the level of resistance in this QTL. Additional SSR markers of the targeted Pi35(t) region were further surveyed in the 190 F2 plants, and Pi35(t) was placed in a 3.5-cM interval flanked by markers RM1216 and RM1003. 相似文献
7.
Application of AFLP, RAPD and ISSR markers to genetic mapping of European and Japanese larch 总被引:25,自引:0,他引:25
A. Arcade F. Anselin P. Faivre Rampant M. C. Lesage L. E. Pâques D. Prat 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(2):299-307
Genetic linkage maps have been increasingly developed for a wide variety of plants, using segregating populations such as
F2s or backcrosses between inbred lines. These pedigrees are rarely available in outbred species like forest trees which have
long generation times. Thus genetic mapping studies have to use peculiar pedigrees and markers in appropriate configurations.
We constructed single-tree genetic linkage maps of European larch (Larix decidua Mill.) and Japanese larch [Larix kaempferi (Lamb.) Carr.] using segregation data from 112 progeny individuals of an hybrid family. A total of 266 markers (114 AFLP,
149 RAPD and 3 ISSR loci) showing a testcross configuration, i.e.heterozygous in one parent and null in the other parent,
were grouped at LOD 4.0, θ=0.3. The maternal parent map (L. decidua)consisted of 117 markers partitioned within 17 linkage groups (1152 cM) and the paternal parent map (L. kaempferi) had 125 markers assembled into 21 linkage groups (1206 cM). The map distance covered by markers was determined by adding
a 34.7-cM independence distance at the end of each group and unlinked marker. It reached 2537 cM and 2997 cM respectively
for European larch and Japanese larch, and represented respectively a 79.6% and 80.8% coverage of the overall genome. A few
3:1 segregating markers were used to identify homologous linkage groups between the European larch and the Japanese larch
genetic maps. The PCR-based molecular markers allowed the construction of genetic maps, thus ensuring a good coverage of the
larch genome for further QTL detection and mapping studies.
Received: 15 March 1999 / Accepted: 29 March 1999 相似文献
8.
A. Lehmensiek A.M. Esterhuizen D. van Staden S. W. Nelson A. E. Retief 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(5):797-803
Bulked segregant analysis was used to identify amplified fragment length polymorphism markers (AFLPs) linked to quantitative
trait loci (QTLs) involved in the resistance to gray leaf spot (GLS) in maize. By using ten AFLP primer combinations 11 polymorphic
markers were identified and converted to sequence- specific PCR markers. Five of the 11 converted AFLPs were linked to three
GLS resistance QTLs. The markers were mapped to maize chromosomes 1, 3 and 5 using existing linkage maps of two commercially
available recombinant inbred-line populations. Converted restriction fragment length polymorphism markers and microsatellite
markers were used to obtain a more-precise localization for the detected QTLs. The QTL on chromosome 1 was localized in bin
1.05/06 and had a LOD score of 21. A variance of 37% was explained by the QTL. Two peaks were visible on chromosome 5, one
was localized in bin 5.03/04 and the other in bin 5.05/06. Both peaks had a LOD score of 5, and 11% of the variance was explained
by the QTLs. A variance of 8–10% was explained by the QTL on chromosome 3 (bin 3.04). The consistency of the QTLs was tested
across two F2 populations planted in consecutive years.
Received: 10.10.00 / Accepted: 26.01.01 相似文献
9.
Stich B Melchinger AE Heckenberger M Möhring J Schechert A Piepho HP 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(7):1167-1179
Association mapping in multiple segregating populations (AMMSP) combines high power to detect QTL in genome-wide approaches
of linkage mapping with high mapping resolution of association mapping. The main objectives of this study were to (1) examine
the applicability of AMMSP in a plant breeding context based on segregating populations of various size of sugar beet (Beta vulgaris L.), (2) compare different biometric approaches for AMMSP, and (3) detect markers with significant main effect across locations
for nine traits in sugar beet. We used 768 F
n
(n = 2, 3, 4) sugar beet genotypes which were randomly derived from 19 crosses among diploid elite sugar beet clones. For all
nine traits, the genotypic and genotype × location interaction variances were highly significant (P < 0.01). Using a one-step AMMSP approach, the total number of significant (P < 0.05) marker-phenotype associations was 44. The identification of genome regions associated with the traits under consideration
indicated that not only segregating populations derived from crosses of parental genotypes in a systematic manner could be
used for AMMSP but also populations routinely derived in plant breeding programs from multiple, related crosses. Furthermore,
our results suggest that data sets, whose size does not permit analysis by the one-step AMMSP approach, might be analyzed
using the two-step approach based on adjusted entry means for each location without losing too much power for detection of
marker-phenotype associations. 相似文献
10.
Holger Budahn Herbert Peterka Magdi Ali Ahmed Mousa Yunhua Ding Shaosong Zhang Jinbin Li 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,118(4):775-782
The beet cyst nematode (Heterodera schachtii Schmidt) can be controlled biologically in highly infected soils of sugar beet rotations using resistant varieties of oil
radish (Raphanus sativus L. ssp. oleiferus DC.) as a green crop. Resistant plants stimulate infective juveniles to invade roots, but prevent them after their penetration
to complete the life cycle. The resistance trait has been transferred successfully to susceptible rapeseed by the addition
of a complete radish chromosome. The aim of the study was to construct a genetic map for radish and to develop resistance-associated
markers. The map with 545 RAPD, dpRAPD, AFLP and SSR markers had a length of 1,517 cM, a mean distance of 2.8 cM and consisted
of nine linkage groups having sizes between 120 and 232 cM. Chromosome-specific markers for the resistance-bearing chromosome
d and the other eight radish chromosomes, developed previously from a series of rapeseed-radish addition lines, were enclosed
as anchor markers. Each of the extra chromosomes in the addition lines could be unambiguously assigned to one of the radish
linkage groups. The QTL analysis of nematode resistance was realized in the intraspecific F2 mapping population derived from a cross between varieties ‘Pegletta’ (nematode resistant) x ‘Siletta Nova’ (susceptible).
A dominant major QTL Hs1
Rph
explaining 46.4% of the phenotypic variability was detected in a proximal position of chromosome d. Radish chromosome-specific anchor markers with known map positions were made available for future recombination experiments
to incorporate segments carrying desired genes as Hs1
Rph
from radish into rapeseed by means of chromosome addition lines. 相似文献
11.
A. W. van Heusden M. Koornneef R. E. Voorrips W. Brüggemann G. Pet R. Vrielink-van Ginkel X. Chen P. Lindhout 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(6):1068-1074
Lycopersicon peruvianum LA2157 originates from 1650 m above sea level and harbours several beneficial traits for cultivated tomatoes such as cold
tolerance, nematode resistance and resistance to bacterial canker (Clavibacter michiganensis ssp. michiganensis). In order to identify quantitative trait loci (QTLs) for bacterial canker resistance, a QTL mapping approach was carried
out in an F2 population derived from the interspecific F1 between Lycopersicon esculentum cv Solentos and L.
peruvianum LA2157. Three QTLs for resistance mapped to chromosomes 5, 7 and 9 respectively. The resistance loci were additive and co-dominant
with the QTL on chromosome 7 explaining the largest part of the variation for resistance in the F2 population. The combination of this QTL with either of the other two QTLs conferred a resistance similar to the level in
the resistant parent L. peruvianum. Some RFLP markers flanking this QTL on chromosome 7 were converted into SCAR markers allowing efficient marker-assisted selection
of plants with high resistance to bacterial canker.
Received: 26 February 1999 / Accepted: 12 March 1999 相似文献
12.
Three interspecific crosses were developed using Cicer arietinum (ICC 4918) as the female parent and wild Cicer species [C. reticulatum - JM 2100, JM 2106 and C. echinospermum - ICCW 44] as the male parent. Cicer arietinum (ICC 4918) × C. reticulatum (JM 2100) cross produced the largest number of F2 plants and was chosen for linkage mapping using Random Amplified Polymorphic DNA (RAPD) primers. A partial linkage map was constructed based upon the segregation of 36 RAPD markers obtained by amplification using 35 primers. The linkage map consists of two linkage groups with 17 linked markers covering a total of 464.9 cM. Analyses also revealed association of three morphological traits with linked RAPD markers. Out of seven morphological traits tested for association with linked markers in the segregating plants, four Quantitative trait loci (QTL) were detected for the trait leaf length and three QTLs each for the traits leaf width and erect plant habit. 相似文献
13.
Development and incorporation of microsatellite markers into the linkage map of sugar beet (Beta vulgaris spp.) 总被引:1,自引:0,他引:1
S. J. Rae C. Aldam I. Dominguez M. Hoebrechts S. R. Barnes K. J. Edwards 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(8):1240-1248
A set of informative simple sequence repeat markers has been identified for use in the marker-assisted breeding of Beta vulgaris. Highly enriched small insert genomic libraries were constructed, consisting of 1536 clones (with inserts of between 250–900
bp). Screening the clones with CA, CT, CAA, CATA and GATA nucleotide-repeat probes revealed positive hybridisation to over
50% of the clones. Of these 340 were sequenced. Primer pairs were designed for sequences flanking the repeats and, of these,
57 pairs revealed length polymorphism with 12 Beta accessions. Heterozygosity levels of the SSR loci ranged from 0.069 to 0.809. Heterozygosity levels were found to be similar
to those detected employing RFLP probes with the same accessions. Phenetic analysis using the markers, indicated relationships
in accordance with known pedigrees. Twenty three of the SSR markers were polymorphic in one or both of two F2 mapping populations, and were placed relative to a framework of RFLP probes. The markers are distributed over all nine linkage
groups of sugar beet.
Received: 14 July 1999 / Accepted: 27 October 1999 相似文献
14.
A. Kaga T. Ishii K. Tsukimoto E. Tokoro O. Kamijima 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(2):207-213
A genetic linkage map was developed with 86 F2 plants derived from an interspecific cross between azuki bean (Vigna angularis, 2n=2x=22) and rice bean (V. umbellata, 2n=2x=22). In total, 14 linkage groups, each containing more than 4 markers, were constructed with one phenotypic, 114 RFLP
and 74 RAPD markers. The total map size was 1702 cM, and the average distance between markers was 9.7 cM. The loci showing
significant deviation from the expected ratio clustered in several linkage groups. Most of the skewed loci were due to the
predominance of rice bean alleles. The azuki-rice bean linkage map was compared with other available maps of Vigna species in subgenus Ceratotropis. Based on the lineage of the common mapped markers, 7 and 16 conserved linkage blocks were found in the interspecific map
of azuki bean ×V. nakashimae and mungbean map, respectively. Although the present map is not fully saturated, it may facilitate gene tagging, QTL mapping
and further useful gene transfer for azuki bean breeding.
Received: 20 March 1999 / Accepted: 29 April 1999 相似文献
15.
Sompong Chankaew Prakit Somta Worawit Sorajjapinun Peerasak Srinives 《Molecular breeding : new strategies in plant improvement》2011,28(2):255-264
Cercospora leaf spot (CLS) caused by the fungus Cercospora canescens Illis & Martin is a serious disease in mungbean (Vigna radiata (L.) Wilczek), and disease can reduce seed yield by up to 50%. We report here for the first time quantitative trait loci
(QTL) mapping for CLS resistance in mungbean. The QTL analysis was conducted using F2 (KPS1 × V4718) and BC1F1 [(KPS1 × V4718) × KPS1] populations developed from crosses between the CLS-resistant mungbean V4718 and CLS-susceptible cultivar
Kamphaeng Saen 1 (KPS1). CLS resistance in F2 populations was evaluated under field conditions during the wet seasons of 2008 and 2009, and resistance in BC1F1 was evaluated under field conditions during the wet season in 2008. Seven hundred and fifty-three simple sequence repeat
(SSR) markers from various legumes were used to assess polymorphism between KPS1 and V4718. Subsequently, 69 polymorphic markers
were analyzed in the F2 and BC1F1 populations. The results of segregation analysis indicated that resistance to CLS is controlled by a single dominant gene,
while composite interval mapping consistently identified one major QTL (qCLS) for CLS resistance on linkage group 3 in both F2 and BC1F1 populations. qCLS was located between markers CEDG117 and VR393, and accounted for 65.5–80.53% of the disease score variation depending on
seasons and populations. An allele from V4718 increased the resistance. The SSR markers flanking qCLS will facilitate transferral of the CLS resistance allele from V4718 into elite mungbean cultivars. 相似文献
16.
Genetic mapping and QTL analysis of horticultural traits in cucumber (<Emphasis Type="Italic">Cucumis sativus</Emphasis> L.) using recombinant inbred lines 总被引:18,自引:0,他引:18
Fazio G Staub JE Stevens MR 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,107(5):864-874
A set of 171 recombinant inbred lines (RIL) were developed from a narrow cross in cucumber (Cucumis sativus L.; 2n = 2x = 14) using the determinate (de), gynoecious (F), standard-sized leaf line G421 and the indeterminate, monoecious, little-leaf (ll) line H-19. A 131-point genetic map was constructed using these RILs and 216 F2 individuals to include 14 SSRs, 24 SCARs, 27 AFLPs, 62 RAPDs, 1 SNP, and three economically important morphological [F (gynoecy), de (determinate habit), ll (little leaf)] markers. Seven linkage groups spanned 706 cM with a mean marker interval of 5.6 cM. The location of F and de was defined by genetic linkage and quantitative trait locus (QTL) analysis to be associated with SSR loci CSWCT28 and CSWCTT14 at 5.0 cM and 0.8 cM, respectively. RIL-based QTL analysis of the number of lateral branches in three environments revealed four location-independent factors that cumulatively explained 42% of the observed phenotypic variation. QTLs conditioning lateral branching (mlb1.1), fruit length/diameter ratio (ldr1.2) and sex expression (sex1.2) were associated with de. Sex expression was influenced by three genomic regions corresponding to F and de both on linkage Group 1, and a third locus (sex6.1) on linkage Group 6. QTLs conditioning the number of fruit per plant (fpl1.2), the number of lateral branches (mlb1.4) and fruit length/diameter ratio (ldr1.3) were associated with ll. The potential value of these marker-trait associations (i.e., yield components) for plant improvement is portended by the relatively high LOD scores (2.6 to 13.0) and associated R2 values (1.5% to 32.4%) that are affiliated with comparatively few genetic factors (perhaps 3 to 10).Communicated by H.C. BeckerMention of trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA and does not imply its approval to the exclusion of other products that may be suitable 相似文献
17.
George T. Cicila Carl Choi Howard Dene Soon Jin Lee John P. Rapp 《Mammalian genome》1999,10(2):112-116
Interval mapping was used to identify putative quantitative trait loci (QTL) for blood pressure and cardiac mass on Chromosome
(Chr) 3 in F1(S × R) × S population of 150 rats raised on an 8% NaCl diet. Two genetic markers 95.7 cM apart, D3Wox3 and D3Mco5 (tightly linked to Edn3), showed ``suggestive' linkage to blood pressure (LOD = 2.0 and 1.8 respectively). In addition, D3Wox3 showed ``suggestive' linkage to heart weight (LOD = 2.5), and D3Mco5 showed ``suggestive' linkage to body weight–adjusted heart weight (LOD = 2.1). Congenic rats (designated S.R-Edn3) were
constructed by introgressing the R-rat Edn3 allele (and flanking loci) into the S strain. On a 2% NaCl diet, S.R-Edn3 rats had lower blood pressure (21.4 mm Hg, P= 0.0005) and heart weight (59 mg, P= 0.0038) compared with S rats, confirming the existence of a blood pressure QTL on Chr 3 near Edn3 even though QTL linkage analysis of blood pressure did not achieve stringent statistical criteria for significance. The results
of the congenic experiment and the large distance between the two putative QTL suggest the presence of at least two independent
blood pressure/cardiac mass QTL detectable on Chr 3 in the Dahl rat model of genetic hypertension.
Received: 24 April 1998 / Accepted: 29 September 1998 相似文献
18.
H. Udagawa Y. Ishimaru F. Li Y. Sato H. Kitashiba T. Nishio 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(4):689-696
In interspecific pollination of Brassica rapa stigmas with Brassica oleracea pollen grains, pollen tubes cannot penetrate stigma tissues. This trait, called interspecific incompatibility, is similar
to self-incompatibility in pollen tube behaviors of rejected pollen grains. Since some B. rapa lines have no interspecific incompatibility, genetic analysis of interspecific incompatibility was performed using two F2 populations. Analysis with an F2 population between an interspecific-incompatible line and a self-compatible cultivar ‘Yellow sarson’ having non-functional
alleles of S-locus genes and MLPK, the stigmas of which are compatible with B. oleracea pollen grains, revealed no involvement of the S locus and MLPK in the difference of their interspecific incompatibility phenotypes. In QTL analysis of the strength of interspecific incompatibility,
three peaks of LOD scores were found, but their LOD scores were as high as the threshold value, and the variance explained
by each QTL was small. QTL analysis using another F2 population derived from selected parents having the highest and lowest levels of interspecific incompatibility revealed five
QTLs with high LOD scores, which did not correspond to those found in the former population. The QTL having the highest LOD
score was found in linkage group A02. The effect of this QTL on interspecific incompatibility was confirmed by analyzing backcrossed
progeny. Based on synteny of this QTL region with Arabidopsis thaliana chromosome 5, a possible candidate gene, which might be involved in interspecific incompatibility, is discussed. 相似文献
19.
Cornelia Lange Lukas Mittermayr Juliane C. Dohm Daniela Holtgräwe Bernd Weisshaar Heinz Himmelbauer 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,121(3):549-565
We describe a novel approach for high-throughput development of genetic markers using representational oligonucleotide microarray analysis. We test the performance of the method in sugar beet (Beta vulgaris L.) as a model for crop plants with little sequence information available. Genomic representations of both parents of a mapping population were hybridized on microarrays containing in total 146,554 custom made oligonucleotides based on sugar beet bacterial artificial chromosome (BAC) end sequences and expressed sequence tags (ESTs). Oligonucleotides showing a signal with one parental line only, were selected as potential marker candidates and placed onto an array, designed for genotyping of 184 F2 individuals from the mapping population. Utilizing known co-dominant anchor markers we obtained 511 new dominant markers (392 derived from BAC end sequences, and 119 from ESTs) distributed over all nine sugar beet linkage groups and calculated genetic maps. Further improvements for large-scale application of the approach are discussed and its feasibility for the cost-effective and flexible generation of genetic markers is presented. 相似文献
20.
Sebastian RL Kearsey MJ King GJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(4):601-609
A segregating population of F1-derived doubled haploid (DH) lines of Brassica oleracea was used to detect and locate QTLs controlling 27 morphological and developmental traits, including leaf, flowering, axillary
bud and stem characters. The population resulted from a cross between two very different B. oleracea crop types, an annual cauliflower and a biennial Brussels sprout. A principal component analysis (PCA), based on line means,
allowed all the traits to be grouped into distinct categories according to the first five Principal Components. These were:
leaf traits (PC1), flowering traits (PC2), axillary bud traits (PC3 and 5) and stem traits (PC4). Between zero and four putative
QTL were located per trait, which individually explained between 6% and 43% of the additive genetic variation, using the multiple-marker
regression approach to QTL mapping. For lamina width, bare petiole length and stem length two QTL with opposite effects were
detected on the same linkage groups. Intra- and inter-specific comparative mapping using RFLP markers identified a QTL on
linkage group O8 accounting for variation in vernalisation, which is probably synonymous with a QTL detected on linkage group
N19 of Brassica napus. In addition, a QTL for petiole length detected on O3 of this study appeared to be homologous to a QTL detected on another
B. oleracea genetic map (Camargo et al. 1995).
Received: 28 March 2001 / Accepted: 25 June 2001 相似文献