A Reptilian Terrarium

The 1/4-inch mesh eliminates the raw nose caused by specimens rubbing their noses on fine screening. The fine screening keeps new-born garter snakes, crickets, and other surprises from escaping. A light fixture and a 15-watt sign bulb will keep your specimens more active, feeding more often, and growing faster. Even a 40-watt bulb can be used with comparative safety. Colored gravels or carpet tiles give a splash of color and are easily cleaned. The double-strength glass adds weight and strength to the top. Extra weight may be added to ensure that there will be no escapees. Cleaning is accomplished by removing both pieces of glass. The terrarium may also be used as a greenhouse.     

Growth factors and tooth development

The effects of various growth factors on tooth development were studied in organ cultures of mouse embryonic tooth germs. Transferrin was shown to be a necessary growth factor for early tooth morphogenesis. Transferrin was required for the development of bud- and early cap-staged teeth, and it was shown to be the only serum protein that was needed by early cap-staged teeth in organ culture. Promotion of tooth morphogenesis and dental cell differentiation was shown to be based on the stimulation of cell proliferation. The roles of polypeptide growth factors in tooth development were studied by adding these factors to the transferrin-containing chemically-defined culture medium which supports early tooth morphogenesis and cell differentiation. Fibroblast growth factor or platelet-derived growth factor did not affect cell proliferation or morphogenesis of tooth germs in culture. On the contrary, epidermal growth factor (EGF) stimulated cell proliferation in tooth explants, but at the same time inhibited tooth morphogenesis and dental cell differentiation. Autoradiographic localization of proliferating cells revealed that dental tissues responded to EGF with different proliferation rates. The responsiveness to EGF was stage-dependent, early cap-staged teeth were sensitive to EGF but late cap-staged and bell-staged teeth developed normally in the presence of EGF in the culture medium. The presence and distribution of receptors for both transferrin and EGF were studied in mouse embryonic teeth at various developmental stages by incubating freshly-separated tooth germs with 125Iodine-labeled transferrin or EGF, and then processing the tissues for autoradiography.(ABSTRACT TRUNCATED AT 250 WORDS)     

TNF signalling in tooth development

Mammalian tooth development has served as an excellent model system to investigate the intricate, interactive mechanisms of patterning, morphogenesis and cytodifferentiation during organogenesis. Teeth develop from interactions between epithelium and neural crest-derived (ecto)mesenchyme that are largely mediated by ligand-receptor signalling. It is well-established that signalling molecules of the Bmp, Fgf, Wnt and Hedgehog families, are involved at multiple stages of tooth development. Recently, however, a specific role for molecules belonging to the TNF-family of ligands in tooth morphogenesis has been identified, suggesting that this pathway, acting to activate NF-kappaB, has played an important role in the development and evolution of tooth number and shape.     

The Control of the Reptilian Gonad

In reptiles, there is adequate evidence to indicate environmentalcontrol of the ovarian cycle through hvpothalamic pathways andthe subsequent release of tropic hormone (s) from the anteriorpituitary. The role of the pineal-parietal complex still remainsto be elucidated. In the hypothalamus there appear to be steroidsensitive areas and both progesterone and estrogen appear tohave important feedback influences upon gonadal growth ovulation,and ovarian steroid production. Cytological studies of the reptilianpituitary indicate similar cell types to those observed in mammalianpituitaries, but thus far, two gonadotrophs cannot be identifiedwith any certainty. Chemical and biological studies of the actionof mammalian gonadotropuis suggest that in reptiles hormoneswhich are FSH-like in mammals are able to stimulate gonadaldevelopment, ovulation, and steroid biosynthesis under certainconditions. Preliminary studies of the chemistry of turtle gonadotropuishave so far demonstrated only one active principle, which ischemically similar to mammalian LH, but is far more active thanthe latter hormone in reptilian systems. Further, the hormonealso has FSH-like activity in the reptile. Thus reptilian gonadaldevelopment can be stimulated by treatment with a variety ofmammalian gonadotropins. Recent studies have indicited an importantrole for growth hormone, acting in concert with gonadotropinand estrogen in the regulation of vitellogenesis and ovariangrowth. Piolactin appears to be an antigonadal agent in reptiles,as does progesterone. The exact manner in which these hormonesexert their antigonadal action remains to be clarified, butat least one site of action is the central nervous system; othersites maybe the liver and the fat depot. Ovarian tissue from reptiles is able to synthesize and secretesteioid hormones by pathways similar to those present in mammalianovaries. Circulating levels of estrogen have not been measured,but progesterone levels in the blood show distinct changes assocntedwith pregnancy and the presence of corpora lutea in the ovary.     

The Evolution of Reptilian Social Behavior

Reptiles display a diversity of behavior that is reflectiveof their evolutionary heritage from fish and amphibians andtheir ancestral contribution to the diversity found in birdsand mammals. Much of the behavior observed in reptiles seemsspecific to the ecological setting within which they live. Asa result, a diversity of behavior is found in each of the groupsof modern reptiles. Recent studies on the social behavior oflizards have proved them capable of a variety of behavioralpostures, sequences, and sociality that exceeds that found insome mammals and birds. While many species of lizards are territorial,others are hierarchial and some have harems. For all those territorialspecies studied, crowding results in increased social interaction,increased aggression, and a switch to hierarchial behavior. While smell and sound may be important stimuli for social behaviorin some reptiles, posture, actions, and especially color appearto be most important in diurnal lizards. Temperature and energystudies suggest that the large extinct dinosaurs probably foughtconsiderably less than commonly portrayed in movies and stories,but were also probably much more brightly colored than commonlyshown in reconstructions.     

ALK5 is essential for tooth germ differentiation during tooth development

ABSTRACT

The TGFβ superfamily of proteins participates in tooth development. TGFβ1 and TGFβ3 regulate odontoblast differentiation and dentin extracellular matrix synthesis. Although the expression of TGFβ family member ligands is well-characterized during mammalian tooth development, less is known about the TGFβ receptor, which is a heteromeric complex consisting of a type I and type II receptors. The molecular mechanism of ALK5 (TGFβR1) in the dental mesenchyme is not clear. We investigated the role of ALK5 in tooth germ mesenchymal cells (TGMCs) from the lower first molar tooth germs of day 15.5 embryonic mice. Human recombinant TGFβ3 protein or an ALK5 inhibitor (SD208) was added to the cells. Cell proliferation was inhibited by SD208 and promoted by TGFβ3. We found that SD208 inhibited TGMCs osteogenesis and dentinogenesis. Both canonical and noncanonical TGFβ signaling pathways participated in the process. TAK1, P-TAK1, p38 and P-p38 showed greater expression and SMAD4 showed less expression when ALK5 was inhibited. Our findings contribute to understanding the role of TGFβ signaling for the differentiation of mesenchymal stem cells derived from dental germ and suggest possible targets for optimizing the use of stem cells of dental origin for tissue regeneration.     

Discussion of the Reptilian Ear

Evidence presented at this symposium lends support to the conceptthat the reptilian auditory apparatus shows major modificationswhich correlate with large taxonomic groups, and that lesseranatomical differences suggestive of adaptive change can beseen at subfamilial levels. Structures concerned with transmission,reception, and dampening of sonic vibratory energy are mostfrequently involved in modifications noted; thus, anatomicaldifferences may account for diverse results of acoustic testingin reptiles. Preliminary investigation of the histology andcytology of certain features of the reptilian cochlear ductindicates considerable specialization at the cellular leveland the need for continuing anatomical and physiological study.     

Growth factors in human tooth development

Our research concerns the immunohistochemical localization of EGF and IGF-I receptors in the tooth germ, using monoclonal antibodies. The results show that in the early phases of human tooth development EGF and IGF-I receptors are present. At bud stage both receptors are localized at dental laminae level, in some epithelial cells of the tooth bud and in some mesenchymal cells. At cap stage the receptors are present in the outer and inner enamel epithelium, and in some cells of stellate reticulum. As far as concerns the mesenchymal cells, some cells of dental papilla in contact with enamel organ, are intensely positive. The immunopositivity is present also in some mesenchymal cells at follicular level. At late cap stage and at early bell stage receptors are not present at inner enamel epithelium level but they can be detectable in the mesenchyma of dental papilla and in some cells of the follicle. On the basis of these results it may be hypothesized that EGF and IGF-I can act as growth factors in the modulation of cellular proliferation and differentiation during the human tooth morphogenesis. Moreover, it is possible that these substances can play a role in the mesenchymal-epithelial interaction in the developing human tooth.     

Form and Function in Reptilian Circulations

Consistent with the great variation in their circulatory morphology,there are distinct variations in the cardiovascular physiologyof extant reptiles. The chelonian and squamate reptiles havea complexly structured heart that includes three partially separatedventricular cava. In most species (under most conditions), theventricle acts as a single pressure pump perfusing both thepulmonary and systemic circuits. However, the varanid lizardsprovide a striking exception. Subtle evolutionary changes incardiac morphology allow the ventricle of the varanid lizardto divide functionally during systole into a low pressure, pulmonarypump and a high pressure, systemic pump. The crocodilians representyet another anatomical and physiological pattern. The ventricleis completely divided into left and right chambers as in homeotherms,but the systemic and pulmonary circuits may still communicatethrough the left aorta that arises from the right ventricle. A fundamental feature of all reptilian circulations is the abilityto regulate the distribution of cardiac output between systemicand pulmonary circuits via central vascular blood shunts.Regardlessof species, mechanisms for regulating intracardiac shuntinginvolve changes in the balance between peripheral resistanceof the pulmonary and systemic circulations, and adjustmentsin cardiac performance per se. Several hypotheses are presentedthat suggest selective advantages for central vascular shuntingin intermittent breathing reptiles with variable body temperatureand metabolic rate.     

Fate of HERS during tooth root development

Tooth root development begins after the completion of crown formation in mammals. Previous studies have shown that Hertwig's epithelial root sheath (HERS) plays an important role in root development, but the fate of HERS has remained unknown. In order to investigate the morphological fate and analyze the dynamic movement of HERS cells in vivo, we generated K14-Cre;R26R mice. HERS cells are detectable on the surface of the root throughout root formation and do not disappear. Most of the HERS cells are attached to the surface of the cementum, and others separate to become the epithelial rest of Malassez. HERS cells secrete extracellular matrix components onto the surface of the dentin before dental follicle cells penetrate the HERS network to contact dentin. HERS cells also participate in the cementum development and may differentiate into cementocytes. During root development, the HERS is not interrupted, and instead the HERS cells continue to communicate with each other through the network structure. Furthermore, HERS cells interact with cranial neural crest derived mesenchyme to guide root development. Taken together, the network of HERS cells is crucial for tooth root development.     

Possible involvement of maspin in tooth development

Maspin is a 42 kDa serine protease inhibitor that possesses tumor suppressive and anti-angiogenic activities. Despite of a huge amount of data concerning the expression pattern of maspin in various tissues and its relevance to the biological properties of a variety of human cancer cells, little is known on the maspin expression in skeletal and tooth tissues. Recently, we reported that maspin may play an important role in extracellular matrix formation in bone by enhancing the accumulation of latent TGF-β in the extracellular matrix. This study was performed to elucidate the possible role of maspin in tooth development. First, an immunohistochemical analysis for human tooth germs at the late bell stage showed the expression of maspin by active ameloblasts and odontoblasts that were forming enamel and dentin, respectively. During rat tooth development, maspin expression was observed for the first time in inner and outer enamel epithelial cells and dental papilla cells at early bell stage. The neutralizing anti-maspin antibody inhibited the proper dental tissue formation in organ cultures of mandibular first molars obtained from 21-day-old rat embryos. In addition, the proliferation of HAT-7 cells, a rat odontogenic epithelial cell line, and human dental papilla cells were suppressed in a dose-dependent manner with anti-maspin antibody. Moreover, RT-PCR analysis showed that the expression of mRNA for tooth-related genes including dentin matrix protein 1, dentin sialophosphoprotein and osteopontin in human dental papilla cells was inhibited when treated with anti-maspin antibody. These findings suggest that maspin expressed in ameloblasts and odontoblasts plays an important physiological role in tooth development through the regulation of matrix formation in dental tissues.     

Fgf signaling is required for zebrafish tooth development

We have investigated fibroblast growth factor (FGF) signaling during the development of the zebrafish pharyngeal dentition with the goal of uncovering novel roles for FGFs in tooth development as well as phylogenetic and topographic diversity in the tooth developmental pathway. We found that the tooth-related expression of several zebrafish genes is similar to that of their mouse orthologs, including both epithelial and mesenchymal markers. Additionally, significant differences in gene expression between zebrafish and mouse teeth are indicated by the apparent lack of fgf8 and pax9 expression in zebrafish tooth germs. FGF receptor inhibition with SU5402 at 32 h blocked dental epithelial morphogenesis and tooth mineralization. While the pharyngeal epithelium remained intact as judged by normal pitx2 expression, not only was the mesenchymal expression of lhx6 and lhx7 eliminated as expected from mouse studies, but the epithelial expression of dlx2a, dlx2b, fgf3, and fgf4 was as well. This latter result provides novel evidence that the dental epithelium is a target of FGF signaling. However, the failure of SU5402 to block localized expression of pitx2 suggests that the earliest steps of tooth initiation are FGF-independent. Investigations of specific FGF ligands with morpholino antisense oligonucleotides revealed only a mild tooth shape phenotype following fgf4 knockdown, while fgf8 inhibition revealed only a subtle down-regulation of dental dlx2b expression with no apparent effect on tooth morphology. Our results suggest redundant FGF signals target the dental epithelium and together are required for dental morphogenesis. Further work will be required to elucidate the nature of these signals, particularly with respect to their origins and whether they act through the mesenchyme.     

Cell fate determination during tooth development and regeneration

Teeth arise from sequential and reciprocal interactions between the oral epithelium and the underlying cranial neural crest‐derived mesenchyme. Their formation involves a precisely orchestrated series of molecular and morphogenetic events, and gives us the opportunity to discover and understand the nature of the signals that direct cell fates and patterning. For that reason, it is important to elucidate how signaling factors work together in a defined number of cells to generate the diverse and precise patterned structures of the mature functional teeth. Over the last decade, substantial research efforts have been directed toward elucidating the molecular mechanisms that control cell fate decisions during tooth development. These efforts have contributed toward the increased knowledge on dental stem cells, and observation of themolecular similarities that exist between tooth development andregeneration. Birth Defects Research (Part C) 87:199–211, 2009. © 2009 Wiley‐Liss, Inc.     

Cellular expression of neurotrophin mRNAs during tooth development

. Target-derived neurotrophins support and sustain peripheral sensory neurons during development. In addition, it has been suggested that these growth factors could have developmental functions in non-neuronal tissues. To further elucidate the possible roles of neurotrophins in tooth morphogenesis and innervation, we have used in-situ hybridization to determine the specific sites of neurotrophin gene activity in pre- and postnatal rat jaws from E16 to P7. All four neurotrophins were expressed during tooth development with specific temporospatial patterns. Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) mRNAs were mainly detected in the dental papilla/pulp in postnatal animals, and the pattern of expression correlated with the onset of dental innervation. In contrast, neurotrophin 3 (NT3) and neurotrophin 4 (NT4) mRNA expression patterns were predominantly epithelial and were strongest during early developmental stages when teeth are not yet innervated. Dental papilla NGF-mRNA expression was first seen in both epithelium and mesenchyme and later shifted to the odontoblast layer and the subodontoblast zone. BDNF-mRNA labeling was present in low levels in the early dental organ, but increased in the pulp and in the odontoblast cell layer of the developing teeth at later developmental stages. Both NT3 and NT4 mRNA were observed in the prenatal oral epithelium and the inner dental epithelium. NT3-mRNA labeling was seen mainly in the cervical loop region, fissure system depressions and cuspal tops, while NT4 mRNA was more evenly distributed in the dental epithelium. At P7, NT3-mRNA labeling was below detection level and NT4 mRNA expression was lower than at prior stages. Complementary to reports on the presence of low-affinity neurotrophin receptor (LANR), trkB and trkC mRNA in the developing teeth, our results suggest that neurotrophins may have multiple functions during tooth morphogenesis. Neurotrophins might participate in epithelial-mesenchymal interactions in early tooth morphogenetic events such as proliferation and differentiation of epithelial and mesenchymal cells. In addition, based on mRNA localization in postnatal animals, we also suggest that NGF and BDNF (beside glial cell line-derived neurotrophic factor) might participate in establishing and maintaining the innervation of the teeth, thus acting as classical neurotrophic factors.     

Molar tooth development in caspase-3 deficient mice

Tooth morphogenesis is accompanied by apoptotic events which show restricted temporospatial patterns suggesting multiple roles in odontogenesis. Dental apoptosis seems to be caspase dependent and caspase-3 has been shown to be activated during dental apoptosis.Caspase-3 mutant mice on different genetic backgrounds were used to investigate alterations in dental apoptosis and molar tooth morphogenesis. Mouse embryos at E15.5 were analyzed to reveal any changes in enamel knots, which are transient structures eliminated by apoptosis. In caspase-3(-/-) mice on the B57BL/6 background, disorganization of the epithelium was found in the original primary enamel knot area and confirmed by altered expression of Shh. Despite this early defect in molar tooth development, these mutants showed correct formation of secondary enamel knots as indicated by Fgf-4 expression. Analyses of adult molar teeth did not reveal any major alterations in tooth shape, enamel structure or pattern when compared to heterozygote littermates. In caspase-3(-/-) mice on the 129X1/SvJ background, no defects in tooth development were found except the position of the upper molars which developed more posteriorly in the oral cavity. This is likely, however, to be a secondary defect caused by a physical squashing of the face by the malformed brain. The results suggest that although caspase-3 becomes activated and may be essential for dental apoptosis, it does not seem fundamental for formation of normal mineralised molar teeth.     

福建省爬行动物区系及地理区划

福建省爬行动物有123种和亚种,隶属2目17科69属.除5种海产龟鳖类及9种海蛇外,其余109种陆栖与半水栖(淡水)的种类中,属东洋界的种类有101种,占92.66%;遍布于古北界和东洋界的种类共有8种,占7.34%.福建省陆栖与半水栖的爬行动物区系可划分为闽北山区丘陵省、闽东丘陵沿海省、闽西低山丘陵省、闽中丘陵平原省和闽南低丘平原省5个地理分布区,对每个地理分布区的自然环境和动物区系的组成和特点分别进行了描述和分析.