蜘蛛对农药敏感性的测定方法

介绍了用点滴法和浸渍法测定蜘蛛对农药敏感性的具体操作过程。溴氰菊酯作用于三突花蛛的半致死量LD50和半致死浓度LC50分别为0．842μg/g,和2．1937mg/L。     

杀虫剂啶虫脒和毒死蜱对捕食蜘蛛血细胞DNA的损伤作用

应用蜘蛛血细胞微核试验和单细胞凝胶电泳试验研究了两种杀虫剂啶虫脒和毒死蜱对蜘蛛头胸部和腹部血细胞DNA的损伤作用。结果表明：在啶虫脒和毒死蜱各供试浓度作用下,对蜘蛛血细胞微核率有明显的影响,与对照组相比有显著性差异(p<0.05,p<0.01);且随着两种农药浓度升高,血细胞微核率显著增加,存在明显的剂量-效应关系(啶虫脒浓度与星豹蛛头胸部血细胞微核率相关系数r=0.9284,腹部为r=0.9071;毒死蜱与星豹蛛头胸部血细胞微核率相关系数r=0.9841,腹部为r=0.9793);啶虫脒和毒死蜱对星豹蛛血细胞DNA损伤有明显的剂量-效应关系(啶虫脒浓度与星豹蛛头胸部血细胞DNA损伤相关系数r=0.9838,腹部为r=0.9834;毒死蜱与星豹蛛头胸部血细胞DNA损伤相关系数r=0.9807,腹部为r=0.9659);且两种农药在同种农药同一浓度作用下,对星豹蛛腹部血细胞微核率和DNA损伤程度要明显大于头胸部。     

蜘蛛抗逆能力的研究

本文对隐蛛科的白斑隐蛛;狼蛛科的拟环纹豹蛛、星豹蛛、真水狼蛛、细毛水狼蛛;卷叶蛛科的猫卷叶蛛;栅蛛科的栓栅蛛;球蛛科的温室希蛛;皿蛛科的草间钻头蛛、难波小黑蛛、隆背微蛛、花腹盖蛛;园蛛科的黄斑园蛛;管巢蛛科的棕管巢蛛;蟹蛛科的三突花蛛;跳蛛科的吉蚁蛛等10科共16种蜘蛛做了在30 ℃恒温条件下的抗逆能力测定.结果表明,蜘蛛是一类抗逆能力较强的动物,只要供给水分,其雌蛛的平均寿命在25 d以上;水在蜘蛛生命活动中具有非常重要的作用,在相同的条件下,如果断食断水,蜘蛛的平均寿命显著缩短,只有6 d左右;由于蜘蛛种类和生活环境的不同,其耐饥饿的能力也表现出很大的差异,一般地面活动的蜘蛛或靠水边活动的蜘蛛耐饥、耐水能力较差,而游猎性蜘蛛,尤其是结网的蜘蛛抗逆能力较强;雌蛛的抗逆能力大于雄蛛;三突花蛛的耐饥能力随着龄期的增加而加强.     

贵州省茶园蜘蛛区系分布特征

经10多年考查和鉴定,记述了贵州省茶区蜘蛛26科204种,其中优势类群园蛛科50种、跳蛛科25种、肖蛸科20种、蟹蛛科14种、平腹蛛科13种,占总种数60%.全省性种类角类肥蛛、黄褐新园蛛、草间小黑蛛、食虫瘤胸蛛、三突花蛛、鞍形花蟹蛛、机敏蝇豹、纵条蝇狮、悦目金蛛、棒络新妇蛛、八斑鞘腹蛛、星豹蛛、棕管巢蛛、斜纹猫蛛、机敏漏斗蛛、浙江豹蛛、兰翠蛛、黑色蝇虎、茶色新园蛛和锥腹肖蛸等64种,占总种数31%.在我国动物区划中,贵州大部属于华中区的西部山地高原亚区,西部少数地区属于西南区的西南山地亚区.结合茶树适生格局,把茶园蜘蛛区系划分为:黔中山地丘陵区(Ⅰ)、黔东低山丘陵盆地区(Ⅱ)、黔南低山河谷区(Ⅲ)、黔北中山峡谷区(Ⅳ)、黔西高原中山区(Ⅴ).Ⅰ区发现蜘蛛21科137种,占全省总种数67%;常见的优势种有角类肥蛛、黄褐新园蛛、灌木新园蛛、青新园蛛和悦目金蛛等37种,占该区蜘蛛总种数27%;特有种为山地艾蛛、畸形艾蛛、对称曲腹蛛、西山新园蛛、小青新园蛛、四点亮腹蛛、桐庐指蛛、锯胸微蛛、黑底狼蛛、中华刺足蛛、裂额银斑蛛、贵州花蟹蛛和三斑花蟹蛛等13种.Ⅱ区发现蜘蛛26科185种,占全省总种数91%;优势种有角类肥蛛、棕管巢蛛、毁坏管巢蛛、斜纹猫蛛和三突花蛛等31种,占该区蜘蛛总种数17%;特有种有中华蟱蛛、棒络新妇蛛、四斑粗螯蛛、风雅丽蛛和江安七纺蛛等58种.Ⅲ区发现蜘蛛15科92种,占总种数45%;优势种为角类肥蛛、草间小黑蛛、食虫瘤胸蛛、驼背额角蛛和八斑鞘腹蛛等18种,占该区蜘蛛总种数20%.特有种为圆腹艾蛛、椭圆新园蛛、横带隆背蛛和凤振粗螯蛛等4种.Ⅳ区发现蜘蛛15科76种,占全省总种数37%;优势种为角类肥蛛、机敏漏斗蛛、机敏蝇豹、纵条蝇狮和黑色蝇虎等18种,占该区蜘蛛总种数24%.Ⅴ区发现蜘蛛14科64种,占总种数31%;优势种为角类肥蛛、黄褐新园蛛、机敏漏斗蛛、机敏蝇豹、黄斑菲蛛等15种,占该区蜘蛛总种数23%;Ⅳ和Ⅴ区无特有种类.Ⅲ区雨、热和光照条件较优越,但其为新辟茶区,蜘蛛种数少于Ⅰ和Ⅱ区.从西北部高寒山区向东部低丘,海拔明显降低,气候从北亚热带过渡到中亚热带,雨量、光照和积温加大,茶树等农林作物增多,茶园蜘蛛种类增多.     

三突花蛛捕食速度的初步研究

本文研究了三突花蛛Misumenops tricuspidatus在不同条件下对果蝇的捕食速度.结果表明,蜘蛛的捕食速度与蜘蛛的步足长度、身体长度无关,而与性别、温度、饥饿程度有显著关系.25 ℃为三突花蛛捕食的最适温度,14 ℃和34 ℃即温度过低或过高时,它的捕食速度显著减慢;三突花蛛在饥饿3～4 d时的捕食速度最快;雄蛛的捕食速度显著大于雌蛛.蜘蛛的共存,如雌蛛-雌蛛、雄蛛-雄蛛、雌蛛-雄蛛共存,对捕食的速度也存在着一定的影响:2雄蛛共存时,各自的捕食速度显著减慢;2雌蛛共存时,各自的捕食速度变化不显著;1雌蛛1雄蛛共存时,雌蛛的捕食速度降低不显著,而雄蛛的捕食速度显著降低.     

弹尾虫单克隆抗体的制备及其在捕食研究中的应用

应用杂交瘤技术制备了针对弹尾虫的单克隆抗体2F10。该抗体的效价为1.024×108,只与灰橄榄长角跳虫、球角跳虫和钩圆跳虫等弹尾虫发生强烈反应而不与稻田常见的其它昆虫和蜘蛛发生交叉反应,具有高度特异性。建立了2F10、HRP-2F10和蜘蛛样品分别稀释4000倍(34.193ng/L)、1500倍(2.4624ng/L)和50倍(50ml/individual)的抗体夹心ELISA检测系统用于检测稻田常见蜘蛛对弹尾虫的捕食作用。其检测灵敏度为1/2头灰橄榄长角跳虫(4.49μg),拟环纹豹蛛捕食1头灰橄榄长角跳虫成虫后,在25℃下猎物的可测定时间为4.5h。应用该检测系统研究了不同稻区常见蜘蛛对2F10的阳性反应率。其中狡蛛、拟环纹豹蛛、纵条蝇狮和纵条蝇虎的阳性反应率显著高于食虫瘤胸蛛、八斑球腹蛛和锥腹肖蛸。     

12种杀虫剂对柑橘大实蝇选择行为的影响及击倒作用

柑橘大实蝇是柑橘上的重要害虫。本文分别利用Y型嗅觉仪和饲喂法测定了柑橘大实蝇成虫对12种杀虫剂的选择行为和击倒作用,以期筛选出田间防治柑橘大实蝇的高效农药,并为研发其诱杀剂提供理论依据。结果表明:12种杀虫剂中,吡虫啉、阿维菌素对雌成虫具有极显著的驱避作用;多杀霉素、丁硫克百威、阿维菌素、氟铃脲对雄成虫具有极显著的驱避作用;多杀霉素、毒死蜱、噻嗪酮对雌成虫具有显著的驱避作用;啶虫脒、噻虫嗪、乙酰甲胺磷、高效氯氟氰菊酯和高效氯氰菊酯对柑橘大实蝇雌雄成虫既无驱避作用也无引诱作用。同种药剂对柑橘大实蝇雌雄成虫的击倒时间无显著差异,不同药剂间对柑橘大实蝇成虫的击倒时间有显著差异,其中啶虫脒的击倒时间(KD₅₀<1 min)最短。综合考虑杀虫剂对柑橘大实蝇选择行为的影响及击倒作用,认为啶虫脒更适宜作为柑橘大实蝇诱杀剂中的杀虫剂。     

除草剂丁草胺对常见稻田蜘蛛毒性的测定及对其生长发育的影响

采用浸渍法测定了除草剂丁草胺对室内饲养的4种稻田蜘蛛拟水狼蛛(Pirata subpiraticus)、拟环纹豹蛛(Pardosa pseudoannulata)、锥腹肖蛸(Tetragnatha maxillosa)和食虫沟瘤蛛(Ummeliata insecticeps)的毒力,以及对拟水狼蛛生长发育的影响.结果表明,高剂量的丁草胺对稻田蜘蛛有较强的杀伤力,丁草胺对4种蜘蛛半致死浓度由高到低依次为:拟环纹豹蛛>拟水狼蛛>锥腹肖蛸>食虫沟瘤蛛,食虫沟瘤蛛对丁草胺最敏感,LC50为9.61605 mg/L.用2mg/L丁草胺溶液作为水源喂养的拟水狼蛛体重、雌雄性比(3:2)、幼蛛成活率(43.4%)均远小于对照组,其3～6龄总历期延长了10d,经统计检验与对照组差异显著(P<0.05),可见丁草胺对蜘蛛生长发育有显著影响.     

稻田拟环纹狼蛛的生态观察

在自然界中,蜘蛛是各种农作物害虫的天敌之一。据不完全统计,在稻、棉田中有40种以上。我县稻、棉田的优势种有拟环纹狼蛛、水狼蛛、草间小黑蛛、管巢蛛、沟渠豹蛛等,它们常守于水稻、棉花的上中下部,捕食量大,每天能捕食数只到十多只害虫,常捕食飞虱、叶蝉、稻纵卷叶螟、稻螟铃等十多种害虫。它吃活虫,不为害水稻。每年从四月上旬到十月下旬均能捕虫,即在早稻、晚稻整个生育期都可利用蜘蛛治虫。我们对     

不同冬季气候条件下的麦田蜘蛛群落结构和优势种繁殖情况

1998～1999年、1999～2000年在武汉地区使用陷阱法系统采样,研究了大麦田蜘蛛群落结构,比较了不同的冬天气候因素对麦田蜘蛛群落结构的影响.使用Berger-Parker优势度指数分析了调查时间段大麦田的地面优势种蜘蛛.结果显示,1998～1999年的优势种蜘蛛为皿蛛科蜘蛛,隆背微蛛Erigone prominens Boes. et Str.在大麦的全生长期均为优势种.1999～2000年,主要的优势种蜘蛛为狼蛛科的类水狼株Pirata piratoides Boes.et Str.、沟渠豹蛛Pardosa laura Karsch和拟环纹豹蛛Pardosa pseudoannulata (Boes.et Str.).另外,通过各优势种蜘蛛雄蛛的个体数变化,探讨了各优势种蜘蛛在田间的交配繁殖情况.结果表明,冬天气候对蜘蛛的交配时期有明显影响.在暖冬天气,大多数种类的交配比冷冬提前.一般情况下,各优势种蜘蛛能在麦田繁殖1代.另外,还对平腹蛛科的2种蜘蛛的雄蛛在田间的活动情况进行了描述.     

Aged pesticide residues are detrimental to agrobiont spiders (Araneae)

Spiders are among the most abundant arthropods in agroecosystems, playing an important role as natural enemies of various pests. In this study we evaluated residual activity of selected pesticides on the mortality and behaviour of four spider species (Dictyna uncinata, Pardosa palustris, Philodromus cespitum and Theridion impressum). We used three pesticides: a herbicide Command (clomazone), and insecticides Decis (deltamethrin) and Nurelle (chlorpyrifos and cypermethrin). Mortality was recorded after exposure of spiders to fresh (2-h), 5, 10, 15 and 20-day-old residues. For each residue mortality was evaluated after 24–72 h. Residual effect differed between preparations and, in some cases, between spiders. All of the Nurelle residues (fresh to 20-day-old) caused 100% mortality in all spider species. Residues of Command were rather harmless (causing <20% mortality) to all spider species as the herbicide activity declined with age. Residues of Decis had species-specific effects as the mortality varied between 0 and 90%. In Dictyna the mortality gradually declined with the age of residues, while in Pardosa the mortality increased. In Philodromus and Theridion the mortality declined up to 10-day-old residues and then increased so that 20-day-old residues caused almost as high mortality as the new ones. Exposure to pesticide residues immediately affected the movement of Pardosa spiders. Residues of Decis and Nurelle decreased spider locomotion, while those of Command increased locomotion in comparison with the control. In another experiment, we studied repellence of fresh pesticide residues to Pardosa spiders. In comparison with the control, spiders stayed a similar time on the surface treated with Command, but four times less on Decis and nine times less on Nurelle-treated surfaces, respectively. In conclusion, aged insecticide residues possess a high activity and can cause long-term decline in the abundance and prolonged behavioural disturbance of spiders in agroecosystems.     

The toxicity of aphicide residues to beneficial invertebrates in cereal crops

The toxicity of dimethoate, deltamethrin and pirimicarb residues to Bembidion lampros and Coccinella septempunctata was evaluated by confining groups of insects to winter wheat foliage and soil for 24 h at different times after treatment in the field. Flag leaf residues were found to be more toxic than first leaf residues: soil residues were the least toxic with pirimicarb showing virtually no soil toxicity. In general, dimethoate and deltamethrin showed similar levels of foliar toxicity with flag leaf toxicity on the first day after treatment being in the range 60–80% for B. lampros; deltamethrin was however, less toxic than dimethoate at ground level. Both of these products were more toxic than pirimicarb. The long-term exposure of insects, surviving the 24 h bioassays, to treated soil at different times following application resulted in further mortality and provided estimates of the maximum levels of mortality that populations of predators might suffer migrating into the crop at different times following application. Dimethoate was shown to be particularly harmful at the current recommended field application rate and reduced doses were proposed to limit the severity of the initial effects.     

Effect of tolerance to insecticides on substrate penetration by Formosan subterranean termites (Isoptera: Rhinotermitidae)

Seven selected insecticides were applied to three substrates and bioassayed for barrier efficacy and toxicity against the Formosan subterranean termite, Coptotermes formosanus Shiraki, originating from colonies that differed in their tolerance to the insecticides. A profound substrate effect was seen with all insecticides tested. Sand was the most difficult substrate to penetrate and caused faster and greater mortality of C. formosanus. Increased tolerance in C. formosanus was accompanied by a decrease in penetration of treated substrata with chlordane, methoxychlor, chlorpyrifos, and deltamethrin. The opposite was true in the case of permethrin and bendiocarb. More tolerance C. formosanus displayed decreased mortality in the presence of chlorpyrifos, deltamethrin, bendiocarb, and fipronil. The opposite was true for permethrin.     

四种生物农药及其与二种化学农药混配对稻飞虱混合种群的防治效果

试验以喷雾法研究4种生物农药及其与2种化学农药组合混用对水稻褐飞虱Nilaparvata lugens(Stal)和白背飞虱Sogatella furcifera(Horvath)混合种群的防治效果。结果表明:0.36%苦参碱AS、0.5%藜芦碱WP、1.8%阿维菌素EC、400亿孢子/克白僵菌WP,分别以1500mL/hm2、3000g/hm2、900mL/hm2、1800g/hm2的制剂用量单独使用,只有阿维菌素效果较好,药后7、14d的防效分别达到了73.3%和82.3%,但与对照药剂48%毒死蜱EC1500mL/hm2和25%噻嗪酮WP750g/hm2的防效差异极显著。而4种生物农药与2种化学农药分别以单用剂量减半混合使用的8个处理中,①苦参碱+毒死蜱、②藜芦碱+毒死蜱、③阿维菌素+毒死蜱、④球孢白僵菌+毒死蜱、⑤苦参碱+噻嗪酮、⑥藜芦碱+噻嗪酮6种组配,药后1d的校正防效为81.8%～89.2%,3d为88.2%～96.5%,7d为95.6%～98.1%,14d为86.0%～97.8%。因此,以上生物农药与化学农药的6种组合混用可以有效防治稻飞虱低龄若虫。     

Human metabolic interactions of environmental chemicals

Investigations utilizing recombinant human xenobiotic-metabolizing enzymes as well as human hepatocytes have revealed a number of interactions not only between different environmental chemicals (ECs) but also between ECs and endogenous metabolites. Organophosphorus insecticides (OPs) are potent inhibitors of the human metabolism of carbaryl, carbofuran, DEET and fipronil, as well as the jet fuel components, nonane and naphthalene. OPs are potent irreversible inhibitors of testosterone metabolism by cytochrome P450 (CYP) 3A4 and of estradiol metabolism by CYP3A4 and CYP1A2. All of these CYP inhibitions are believed to be due to the release of reactive sulfur during CYP-catalyzed oxidative desulfuration. It has also been shown that the esterase(s) responsible for the initial step in permethrin metabolism in human liver is inhibited by both chlorpyrifos oxon and carbaryl. A number of pesticides, including chlorpyrifos, fipronil and permethrin, and the repellent, DEET, have been shown to be inducers of CYP isoforms in human hepatocytes, with fipronil being the most potent. Several agrochemicals, including fipronil and the pyrethroids, permethrin and deltamethrin, show toxicity toward human hepatocytes with fipronil being the most potent in this regard. Endosulfan-alpha, which has shown promise as a model substrate for phenotyping CYP3A4 and CYP2B6 in human liver microsomes, is also an inducer of CYP2B6, acting through the PXR receptor.     

四种基质表面上常规杀虫剂对四纹豆象的药效评价（英文）

在害虫治理中, 在消费或贮藏粮食加工产品的建筑设施或场所进行害虫防治需要将杀虫剂施用在各种基质表面上。为了测定不同基质表面上杀虫剂的药效, 将四纹豆象Callosobruchus maculatus (F.)成虫接触田间推荐剂量的阿维菌素、 溴氰菊酯和毒死蜱。结果表明： 施用在玻璃、 瓷砖、 塑料和纸盘表面上, 阿维菌素对四纹豆象成虫的致死率分别为63.33%, 22.41%, 12.9%和11.9%, 而溴氰菊酯在这4种基质表面上对四纹豆象成虫的致死率分别为 55%, 44.2%, 41.3%和 37.4%。在所有基质表面上接触毒死蜱, 四纹豆象成虫的死亡率均为100%。对数据进行的Probit 分析表明, 毒死蜱制剂在玻璃、 瓷砖、 塑料和纸盘上对四纹豆象成虫 的LC₅₀ 值分别为 8.66, 13.6, 29.16和 56.5 μg/mL, 阿维菌素制剂的相应数值分别为119.4, 446.2, 774.2 和 836.4 μg/mL, 溴氰菊酯制剂的相应数值分别为 1 008, 1 131, 1 210和 1 336 μg/mL。据此推断, 毒死蜱对四纹豆象的毒性最强, 且在玻璃、 瓷砖、 塑料和纸盘表面上的毒性依次降低。     

Biochemical effects of chlorpyrifos and deltamethrin on altered antioxidative defense mechanisms and lipid peroxidation in rat liver

Pesticides such as organophosphorus and organochlorine compounds commonly used in agriculture for achieving better quality products are toxic substances and lead to generation of reactive oxygen species (ROS) which have harmful effects on human health. While pyrethroid pesticides are used in preference to organophosphates and organochlorines due to their high effectiveness, low toxicity to non-target organisms and easy biodegradability, they may also produce oxidative stress. Thus, we investigated the effects of chlorpyrifos (CP, an organophosphate) and deltamethrin (DM, a pyrethroid pesticide) treatments at low and high doses on lipid peroxidation (LPO) and antioxidant enzyme activities such as SOD, GSH-Px and CAT in rat liver following 16 weeks exposure. Antioxidative defence mechanisms and lipid peroxidation in rat liver tissues display different responses depending on different pesticide treatments and doses. Biochemical analysis showed that administrations of the chlorpyrifos and deltamethrin cause liver damage. In the present study, we observed that lipid peroxidation levels are higher at high doses than at low doses, but DM caused more pronounced increase than CP. Experimentally, we have also observed that oxidant-antioxidant balance is more affected by deltamethrin treatment than by chlorpyrifos.     

Toxicity of pesticides to <Emphasis Type="Italic">Tamarixia radiata</Emphasis>, a parasitoid of the Asian citrus psyllid

Sixteen pesticides including two fungicides were evaluated for toxicity to adult Tamarixia radiata (Waterston) (Hymenoptera: Eulophidae), a parasitoid of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae). Percentage mortality data were evaluated to generally assess IPM-compatibility of the pesticides with adult parasitoids. The following were found to be least compatible with (most toxic to) adult T. radiata based on the toxicity of direct sprays and potential long residual life on leaves: carbaryl, chlorpyrifos, and fenpropathrin. Although highly toxic to the parasitoid as direct sprays or freshly dried residues, each of the following was more compatible with T. radiata because the toxicity of residues of these pesticides was either low at one to three days after application or relatively non-persistent: abamectin, chenopodium oil, fenpyroximate, and spirotetramat. Depending on environmental conditions, imidacloprid (foliar-applied), phosmet, pyridaben, sulfur and 435 spray oil might also be somewhat more compatible for the same reasons. The pesticides that consistently appeared to be most compatible with T. radiata were aluminum tris, copper hydroxide, diflubenzuron, and kaolin clay (Surround WP).     

湖北潜江农田景观中步甲和蜘蛛的群落结构

1995年6～10月在湖北省潜江市郊用陷阱法研究了不同农田景观元素中步甲和蜘蛛的群落结构,结果表明：10类农田景观元素中的五类景观元素,即稻田边缘,农林间作地,不打药的滩田,少打药的旱田和植被丰富的庭院菜地分别在步甲和蜘蛛的贮存上有重要意义。1)稻田边缘步甲和蜘蛛物种的多样性指数和物种数均最高。2)农林间作地步甲和蜘蛛的均匀度居首位,多样性指数位居第二。3)不打药的滩田步甲和蜘蛛的发生数量多,步甲的主要物种为爪哇屁步甲Pheropsophus javanus和耶屁步甲.Jessoensis。蜘蛛的主要物种为星豹蛛Pardosa astrigera。4)少打药的旱田发生的步甲数量多,主要物种为大劫步甲Lesticus magnus。5)植被丰富的庭园菜地发生的蜘蛛数量多,主要蜘蛛物种为沟渠豹蛛Pardosa laura。步甲或蜘蛛物种和景观元素的对应分析表明了优势步甲大劫步甲,爪哇屁步甲,耶屁步甲和优势蜘蛛沟渠豹蛛,星豹蛛,雾豹蛛Pardosa nebulosa,拟环纹豹蛛 P.Pesudoannulata和类水狼蛛Pirata piratoides各自的适生环境,同时也表明了这些景观元 素与常规管理农田(如水稻田,商业性菜地和棉田)之间在步甲、蜘蛛物种上的联系,这为农田景观的合理设计,保证农业的持续发展提供了参考。     

Toxicity and efficacy of selected pesticides and new acaricides to stored product mites (Acari: Acaridida)

Stored product mites can often infest stored products, but currently there is little information regarding the efficacy of pesticides that can be used for control. In this study we evaluated several common pesticides formulated from single active ingredients (a.i.) or commercially available mixtures (chlorpyrifos, deltamethrin, beta-cyfluthrin, and a combination of deltamethrin and S-bioallethrin), plus an acaricide composed of permethrin, pyriproxyfen and benzyl benzolate, for efficacy against Acarus siro, Tyrophagus putrescentiae, and Aleuroglyphus ovatus. The pesticides were incorporated into the mite diets in a dose range of 10–1000 μg a.i. g⁻¹ diet. Concentrations for suppression of 50 and 90% population growth and eradication (rC₀) of mites were fit to linear regression models. None of the tested pesticides gave complete eradication of A. siro, which was the most tolerant of the three mite species tested. The most effective pesticide Allergoff 175 CS was a combination product (a nano-capsule suspension of permethrin, pyriproxyfen and benzyl benzolate) labeled for dust mites, with rC₀ range of 463–2453 μg a.i. (permethrin) g⁻¹ diet depending on the species. Least effective were chlorpyrifos and deltamethrin.