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1.
From studies on polyols in lens of galactose-fed guinea pigs, r-galactono-1,4-lactone was found, which proves the presence of galactonic acid as a product of galactose oxidation, by gas liquid chromatography and mass spectrometry. The content of this component was one tenth of that of galactitol. In vitro culture of rat lens in 30 mM galactose-loaded media demonstrated the formation of the lactone. The significance of the lactone was discussed with respect to the galactose metabolism in lens. 相似文献
2.
The metabolic fate of intravenously injected peptide-bound chondroitin sulphate in the rat.
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The degradation of intravenously administered chondroitin sulphate-peptide, obtained by trypsin digestion of rat cartilage preparations labelled in vitro with 35S (and, in some cases, with 3H), was studied in rats. As with free chains of chondroitin sulphate, the major site of accumulation and degradation in the body was the liver, although peptide-linked chains were taken up more rapidly than free chains. In the first 2h after intravenous injection of a chondroitin sulphate-peptide fraction, labelled macromolecular components were excreted in the urine. These were shown to be chondroitin sulphate-peptide of the same degree of sulphation but of smaller average size than the injected material. A similar observation was made when free chains of chondroitin sulphate from the same source were administered intravenously. An isolated perfused rat kidney failed to de-sulphate circulating chondroitin sulphate-peptide, but a component of lower average molecular weight was excreted in the urine. When a chondroitin sulphate-peptide fraction of relatively larger hydrodynamic volume was administered, very little chondroitin sulphate appeared in the urine in the first 2h. It was concluded that, depending on size and/or peptide content, the chondroitin sulphate-peptide released from connective tissues into the circulation would probably be subjected to one of two alternative fates. The smaller fragments are more likely to be excreted in the urine, whereas the larger ones are taken up by the liver and there degraded to inorganic sulphate and undefined carbohydrate components. 相似文献
3.
Inositol changes in nerve and lens of galactose fed rats 总被引:3,自引:2,他引:1
Abstract— —(1) Major organs of rats fed a 40 per cent galactose diet for one month were surveyed for changes in free myo-inositol. The levels were reduced only in lens and sciatic nerve. (2) In nerve and lens the low myo-inositol level was associated with high concentrations of galactitol and increased water content. (3) In nerve and lens myo-inositol levels returned towards normal during a period of 1–2 weeks in which animals were fed a regular diet again. The pattern of change showed (a) an initial lag period, and (b) return to normal after galactitol concentrations had fallen to a low level and at the same time that water content returned to normal. (4) In nerve there were changes in scyllo-inositol levels that paralleled those in myoinositol. (5) The evidence suggests that the inositol changes were related to the osmotic effects of galactitol accumulation rather than to a direct inhibition of myo-inositol synthesis or uptake. 相似文献
4.
When administered to rats, echitamine was absorbed rapidly from the tissues and was detected in circulation within 30 min.
The drug level reached a maximum value by 2 h and then decreased steadily. The drug had completely disappeared from the blood
in 6 h. The presence of echitamine was observed within 2 h in urine and the maximum amount of drug was excreted between 2
and 4 h. About 90% of the drug was excreted in urine in 24 h and the drug could not be detected in urine after 48 h. Along
with echitamine, its metabolites were also detected in the urine. Plumbagin was not detected in blood upto 24 h when administered
into rats. The drug was detected in urine within 4 h after administration; a major portion of the drug was excreted in urine
by 24 h and traces of the drug were observed in the urine even after 48 h. 相似文献
5.
Rats fed a high galactose diet (40%, ) for 72 h excreted more than 170 μmol of galactonic acid per day in the urine and accumulated galactonic acid in several tissues, especially liver, intestine, heart, and kidney. Rat liver microsomes incubated in the presence of 30 mm galactose produced galactonic acid. The product of the oxidation of d-galactose was identified as galactonic acid by combined gas-liquid chromatography- mass spectrometry analysis of the trimethylsilyl derivative. Optimal activity was observed at pH 8.0 and was inhibited by heavy metal ions, sulfhydryl reagents, cyanide in the absence of substrate, and various drugs. The apparent Km for galactose was 32.9 mm and V was 160 nmol galactonic acid/4 h/mg microsomal protein. The highest galactose oxidizing specific activity was found in the microsomal fraction; specific activity in the mitochondrial fraction was one half of that in the microsomal fraction, and the soluble fraction had none. The activity was specific for d-galactose, although unidentified oxidation products of d-altrose, d-talose, and 2-deoxy-d-galactose were detected by gas chromatography. Oxidation of galactose did not require oxygen. The addition of NAD+ and NADP+ to the incubation system had little effect on the galactose oxidation; FAD and FMN inhibited the activity. Galactose-dependent formation of hydrogen peroxide was demonstrated during the incubation period. Microsomes from rats and mice contained similar galactose-oxidizing activity whereas those from bovine and chicken liver sources contained 45.3 and 5.4%, respectively. The activity was not detectable in microsomes from guinea pig liver. A liver sample, obtained at autopsy of a galactosemia patient, contained 0.18 μmol of galactonate/g suggesting that an enzyme system similar to that described herein may be responsible for the production of galactonate in human galactosemics. 相似文献
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8.
Radioactive coenzyme Q(10) ([(3)H]CoQ) was synthesized in a way that the metabolites produced retained the radioactivity. Administration of the lipid to rats intraperitoneally resulted in an efficient uptake into the circulation, with high concentrations found in spleen, liver, and white blood cells; lower concentrations in adrenals, ovaries, thymus, and heart; and practically no uptake in kidney, muscle, and brain. In liver homogenate most [(3)H]CoQ appeared in the organelles, but it was also present in the cytosol and transport vesicles. Mitochondria, purified on a metrizamide gradient, had a very low concentration of [(3)H]CoQ, which was mainly present in the lysosomes. All organs that took up the labeled lipid also contained water-soluble metabolites. The majority of metabolites excreted through the kidney and appeared in the urine. Some metabolites were also present in the feces, which further contained nonmetabolized [(3)H]CoQ, excreted through the bile. The major metabolites were purified from the urine, and the mass spectrometric fragmentation showed that these compounds, containing the ring with a short side chain, are phosphorylated. Thus, the results demonstrate that CoQ is metabolized in all tissues, the metabolites are phosphorylated in the cells, transported in the blood to the kidney, and excreted into the urine. 相似文献
9.
15N-ammonia was intraportally infused for 6 hr into chickens fed 5% or 20% protein diet to examine the time course of urinary excretion of intraportal ammonia and dietary effects on it. Urinary ammonia increased linearly for the first hour to the same extent in both dietary groups and thereafter further in the low-protein group. Urinary uric acid derived from the intraportal ammonia adaptively increased and reached a steady state level within 1.5 hr. This level was four times higher in the high-protein group. The infused ammonia was excreted into urine as both ammonia and uric acid, in relatively high proportions in the chickens fed the low-protein diet but was almost all excreted as uric acid in those fed the high-protein diet. 相似文献
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11.
Natural vitamin E includes four tocopherols and four tocotrienols. RRR-alpha-tocopherol is the most abundant form in nature and has the highest biological activity. Although vitamin E is the main lipid-soluble antioxidant in the body, not all its properties can be assigned to this action. As antioxidant, vitamin E acts in cell membranes where prevents the propagation of free radical reactions, although it has been also shown to have pro-oxidant activity. Non-radical oxidation products are formed by the reaction between alpha-tocopheryl radical and other free radicals, which are conjugated to glucuronic acid and excreted through the bile or urine. Vitamin E is transported in plasma lipoproteins. After its intestinal absorption vitamin E is packaged into chylomicrons, which along the lymphatic pathway are secreted into the systemic circulation. By the action of lipoprotein lipase (LPL), part of the tocopherols transported in chylomicrons are taken up by extrahepatic tissues, and the remnant chylomicrons transport the remaining tocopherols to the liver. Here, by the action of the "alpha-tocopherol transfer protein", a major proportion of alpha-tocopherol is incorporated into nascent very low density lipoproteins (VLDL), whereas the excess of alpha-tocopherol plus the other forms of vitamin E are excreted in bile. Once secreted into the circulation, VLDL are converted into IDL and LDL by the action of LPL, and the excess of surface components, including alpha-tocopherol, are transferred to HDL. Besides the LPL action, the delivery of alpha-tocopherol to tissues takes place by the uptake of lipoproteins by different tissues throughout their corresponding receptors. Although we have already a substantial information on the action, effects and metabolism of vitamin E, there are still several questions open. The most intriguing is its interaction with other antioxidants that may explain how foods containing small amounts of vitamin E provide greater benefits than larger doses of vitamin E alone. 相似文献
12.
Lim CK Wong WP Lee SM Chew SF Ip YK 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》2004,174(7):555-564
This study aimed to elucidate the role of urea synthesis in the slender African lungfish Protopterus dolloi in detoxifying ammonia after feeding. There were significant increases in the rate of ammonia excretion in P. dolloi between hours 6 and 15 after feeding. Simultaneously, there were significant increases in urea excretion rates between hours 3 and 18. Consequently, the percentage of total nitrogen (N) excreted as urea N increased to ~60% between hours 12 and 21 post-feeding. Hence, after feeding, the normally ammonotelic P. dolloi became ureotelic. Approximately 41% of the N intake from food was excreted within 24 h by P. dolloi, 55% of which was in the form of urea N. At hour 12 post-feeding, the accumulation of urea N was greater than the accumulation of ammonia N in various tissues, which indicates that feeding led to an increase in the rate of urea synthesis. This is contrary to results reported previously on the infusion of ammonia into the peritoneal cavity of the marine dogfish shark, in which a significant portion of the exogenous ammonia was excreted as ammonia. In contrast, feeding is more likely to induce urea synthesis, which is energy intensive, because feeding provides an ample supply of energy resources and leads to the production of ammonia intracellularly in the liver. The capacity of P. dolloi to synthesize urea effectively prevented a postprandial surge in the plasma ammonia level as reported elsewhere for other non-ureogenic teleosts. However, there was a significant increase in the glutamine content in the brain at hour 24, indicating that the brain had to defend against ammonia toxicity after feeding.Communicated by I.D. Hume 相似文献
13.
B Ia Medovar 《Ukrainski? biokhimicheski? zhurnal》1978,50(4):450-454
The content of urea was studied in protein-free filtrates of the liver kidneys, skeletal muscles, myocardium, spleen, brain tissues and blood serum as well as in urine of 1, 3, 6, 12 and 24-month rats. It is shown that at the age of 6 months the content of urine in most tissues under study is significantly decreased (by 42-73%), at the age of 12 months in the spleen and at the age of 24 months in the brain tissues as compared to the one-month animals. The level of urine decrease in the liver and brain tissues of 24-month animals is less pronounced than in other tissues, that corresponds to age peculiarities of their protein metabolism. A decrease of blood consumption per weight unit and a relative increase in the amount of nitrogen excreted with urine are observed with ageing. The arginase activity in the liver decreases essentially only in 3-month animals. A conclusion is drawn that peculiarities of food consumption and the character of changes in the urea content in tissues and urine are adaptation manifestation of an age decrease in the intensity of nitrogen metabolism and protein demand of the organism. 相似文献
14.
The effect of in vivo lipid peroxidation on the excretion of immunoreactive prostaglandin E2 (PGE2) in the urine of rats was studied. Weanling, male Sprague-Dawley rats were fed a vitamin E-deficient diet containing 10% tocopherol-stripped corn oil (CO) or 5% cod liver oil (CLO) with or without 40 mg dl-alpha-tocopheryl acetate/kg. To induce a high, sustained level of lipid peroxidation, some rats were injected intraperitoneally with 100 mg of iron as iron dextran after 10 days of feeding. Iron overload stimulated in vivo lipid peroxidation in rats, as measured by the increase in expired ethane and pentane. Dietary vitamin E reversed this effect. Rats fed the CLO diet excreted 9.5-fold more urinary thiobarbituric acid-reactive substances (TBARS) than did rats fed the CO diet. Iron overload increased the excretion of TBARS in the urine of rats fed the CO diet, but not in urine of rats fed the CLO diet. Dietary vitamin E decreased TBARS in the urine of rats fed either the CO or the CLO diet. Iron overload decreased by 40% the urinary excretion of PGE2 by rats fed the CO diet, and dietary vitamin E did not reverse this effect. Iron overload had no statistically significant effect on urinary excretion of PGE2 by rats fed the CLO diet. A high level of lipid peroxidation occurred in iron-treated rats, as evidenced by an increase in alkane production and in TBARS in urine in this study, and by an increase in alkane production by slices of kidney from iron-treated rats in a previous study [V. C. Gavino, C. J. Dillard, and A. L. Tappel (1984) Arch. Biochem. Biophys. 233, 741-747]. Since PGE2 excretion in urine was not correlated with these effects, lipid peroxidation appears not to be a major factor in renal PGE2 flux. 相似文献
15.
Ichiro Koshiishi Yoshie Mamura Ji Liu Toshio Imanari 《Biochimica et Biophysica Acta (BBA)/General Subjects》1998,1425(1):209-214
In our previous paper (Biochim. Biophys. Acta 1379 (1998) 257–263), we demonstrated that bicarbonate promotes a cleavage of lactone ring of dehydroascorbate (DHA) on the basis of in vitro experiments. In the present study, we examined the degradation of DHA in blood circulation in vivo by using a high-performance liquid chromatographic method for the determination of ascorbate (AsA), DHA and 2,3-diketogulonate (2,3-DKG), which required no pretreatment of biological fluids. When DHA was intravenously administered to rats, a rapid disappearance of DHA (t1/2<1 min) and a concomitant appearance of 2,3-DKG in blood circulation were observed. Approximately 90% of the administered DHA were excreted into urine as resulting 2,3-DKG (55%) and AsA (31%), respectively. Furthermore, we elucidated that rat plasma lacks an enzyme having an aldonolactonase-like activity. The result of the present study suggests that this DHA disappearance is a function of both a chemical degradation to 2,3-DKG and a reduction to AsA. 相似文献
16.
Metabolism of orally administered rac-1-O-[1'-14C]dodecylglycerol and nutritional effects of dietary rac-1-O-dodecylglycerol in mice 总被引:2,自引:0,他引:2
N Weber 《Journal of lipid research》1985,26(12):1412-1420
The metabolism of orally administered rac-1-O-[1'-14C]dodecylglycerol was investigated in mice. The substrate was rapidly absorbed in the intestine and then incorporated into ether glycerolipids of various organs and tissues in high proportions. In intestine and liver, however, large amounts of rac-1-O-[1'-14C]dodecylglycerol were catabolized by oxidative cleavage of the ether bond followed by degradation of the radioactive cleavage product, i.e., lauric acid, to water-soluble metabolites that were excreted in the urine at a fast rate. The feeding of a rac-1-O-dodecylglycerol-containing diet (1 g rac-1-O-dodecylglycerol/kg body weight X day) given over a period of 4 weeks did not significantly alter body weights or organ weights of mice. Analysis of total lipids revealed that high proportions of the substrate were incorporated into ether lipids of all organs and tissues during the feeding period, generally accompanied by a remarkable increase in saturated acyl moieties and a concomitant decrease of linoleoyl moieties of total lipids. Yet, 4 weeks after removal of the rac-1-O-dodecylglycerol-containing diet, the lipids of murine organs and tissues showed a close resemblance to those of the control group. 相似文献
17.
The effect of oral administration of galactose, lactose, and sucrose and intravenous injection of galactose on the urinary excretion of blood-group-active oligosaccharides has been studied. Galactose given either as the free sugar, a glycoside (lactose) or a constituent of normal diet was an absolute requirement for the formation and excretion of A-trisaccharide, B-trisaccharide and 2'-fucosylgalactose in blood group A, B and O(H) secretors, respectively. Great individual variation was seen in the amounts of galactose-dependent oligosaccharides excreted. Injection of galactose resulted in excretion of 3-59% of the amount of oligosaccharide formed after oral administration to the same individual. The mean ratio A-trisaccharide/B-trisaccharide was 2.7 in four blood-group-A1B secretors and 0.22 in three A2B secretors and can thus serve as a parameter for chemical differentiation between the two blood groups. The excretion of larger blood-group-active oligosaccharides, including the A-pentasaccharide, the B-pentasaccharide and lactodifucotetraose, that are normal components in urine from, respectively, starved A, B, and H secretors, was about the same after oral administration of galactose or lactose. The B-trisaccharide was the only oligosaccharide detected in plasma after oral galactose administration to a blood-group-B secretor individual. The concentration was 0.38 mg/l of plasma. 相似文献
18.
A group of five children with kwashiorkor, seven with marasmic kwashiorkor and one underweight child were given an aflatoxin-free diet consisting of maize meal and milk powder. Blood specimens were collected on admission; on day 4 and 10, 24 hour urine and stool samples were collected for the first ten days. Serum, urine and stool samples were analysed for aflatoxins using high performance liquid chromatography with fluorescent detection, after various extraction and clean-up procedures. The children with kwashiorkor and marasmic kwashiorkor excreted aflatoxins in stools for up to 9 and 6 days after admission respectively. No aflatoxins were detected in the stools or urine of the underweight child. In kwashiorkor, urinary excretion ceased after 2 days, while in marasmic kwashiorkor urinary excretion persisted for 4 days. In stools, B1 was the type of aflatoxin detected most frequently in kwashiorkor and least frequently in marasmic kwashiorkor. Aflatoxin M2 was frequently detected in the stools of both groups of children.Estimates of the total amount of aflatoxin excreted by kwashiorkor and marasmic kwashiorkor indicate that these children were harbouring up to 4 g/kg body weight at the time of admission.These findings establish that aflatoxins accumulate in body fluids and tissues in kwashiorkor and marasmic kwashiorkor which is only slowly eliminated. 相似文献
19.
Ochratoxin A: an important western Canadian storage mycotoxin 总被引:2,自引:0,他引:2
R R Marquardt A Frohlich D Abramson 《Canadian journal of physiology and pharmacology》1990,68(7):991-999
Ochratoxin A (OA) is a mycotoxin produced by certain species of storage fungi of the Penicillium and Aspergillus genera. Toxin production by these fungi is influenced by species and even strain of fungi, time and temperature of incubation, moisture content of substrate, and type of substrate. OA has been shown to occur in various grains, cereals and other plant products, animal feeds, meats, and human tissues in countries throughout the world. Of interest is the discovery of OA in a high percentage of blood from humans in Germany. OA is acutely toxic to many different animals and in addition to being a nephrotoxin, it is a hepatotoxin, a teratogen, a very potent carcinogen, possibly a mutagen, and an immunosuppressive agent. OA is rapidly absorbed throughout the entire gastrointestinal tract and distributes itself in the body as a two-compartment open model and has a particular high affinity for serum albumin. OA is hydrolyzed by the intestinal microflora into nontoxic compounds (7-carboxy-5-chloro-8-hydroxy-3,4-dihydro-3R-methylisocoumarin (O alpha) and phenylalanine). It is excreted as either OA, hydroxylated OA, or O alpha in both the urine and feces. OA appears to exert its toxic effect by promoting an increased level of lipid peroxidation, by inhibition of an amino acylation reaction, and possibly by conversion into metabolites that are capable of binding DNA. These in turn cause other secondary effects associated with OA. It would appear that this compound presents a true potential hazard for humans as its occurrence is wide spread and it is highly carcinogenic. 相似文献
20.
In vivo stability and kinetics of absorption and disposition of 3'' phosphopropyl amine oligonucleotides. 总被引:7,自引:5,他引:2
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J G Zendegui K M Vasquez J H Tinsley D J Kessler M E Hogan 《Nucleic acids research》1992,20(2):307-314
Development of oligonucleotide derivatives as therapeutic agents requires an understanding of their pharmacokinetic behavior. The in vivo disposition and stability of a prototype of such compounds are reported here. The compound studied, a relatively G-rich 38 base 3' phosphopropyl amine oligonucleotide (TFO-1), was cleared from the circulation with a half-life of approximately 10 minutes, displaying distribution kinetics consistent with a two compartment model. TFO-1 was also readily absorbed into circulation from the peritoneal cavity. All tissues examined except brain accumulated the compound reaching concentrations calculated to be in the micromolar range. TFO-1 was found to be stable in circulation and in tissues in that a large fraction of intact material was detected 8 hours after injection, as assessed by gel electrophoresis. Approximately 20-30% of the injected dose was excreted in the urine over an 8 hour period. These results suggest that G-rich oligonucleotides, minimally modified at the 3' end, are relatively stable in vivo and have distribution kinetics favorable to use as therapeutic agents. 相似文献