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Zhang Y  Mao B 《遗传学报》2010,37(9):637-645
The secreted Wnt signaling inhibitor Dickkopf1(Dkk1)plays key role in vertebrate head induction.Its receptor Kremen synergizes with Dkkl in Wnt inhibition.Here we have carried out expression and functional studies of the Dkk and Kremen genes in amphioxus(Branchiostoma belcheri).During embryonic and larval development,BbDkk1/2/4 is expressed in the posterior mesoendoderm,anterior somatic mesoderm and the pharyngeal regions.Its expression becomes restricted to the pharyngeal region on the left side at larval stages.In 45 h larvae,BbDkk1/2/4 is expressed specifically in the cerebral vesicle.BbDkk3 was only detected at larval stages in the mid-intestine region.Seven Kremen related genes were identified in the genome of the Florida amphioxus(Branchiostoma floridae),clustered in 4scaffolds,and are designated Kremen1-4 and Kremen-like 1-3,respectively.In B.belcheri,Kremenl is strongly expressed in the mesoendoderm during early development and Kremen3 is expressed asymmetrically in spots in the larval pharyngeal region.In luciferase reporter assays,BbDkk1/2/4 can strongly inhibit Writ signaling,while BbDkk3,BbKremen1 and BbKremen3 can not.No co-operative effect was observed between amphioxus Dkk1/2/4 and Kremens,suggesting that the interaction between Dkk and Kremen likely originated later during evolution.  相似文献   

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Dopamine receptors function to control many aspects of motor control and other forms of behaviour in both vertebrates and invertebrates. They can be divided into two main groups (D1 and D2) based on sequence similarity, ligand affinity and effector coupling. However, little is known about the pharmacology and functionality of dopamine receptors in the deuterostomian invertebrates, such as the cephalochordate amphioxus ( Branchiostoma floridae) which has recently been placed as the most basal of all the chordates. A bioinformatic study shows that amphioxus has at least three dopamine D1-like receptor sequences. One of these receptors, AmphiD1/β, was found to have high levels of sequence similarity to both vertebrate D1 receptors and to β-adrenergic receptors. Here, we report on the cloning of AmphiD1/β from an adult amphioxus cDNA library, and its pharmacological characterization subsequent to its expression in both mammalian cell lines and Xenopus oocytes. It was found that AmphiD1/β has a similar pharmacology to vertebrate D1 receptors, including responding to benzodiazepine ligands. The pharmacology of the receptor exhibits 'agonist-specific coupling' depending upon the second messenger pathway to which it is linked. Moreover, no pharmacological characteristics were observed to suggest that AmphiD1/β may be an amphioxus orthologue of vertebrate β-adrenergic receptors.  相似文献   

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李建伟  林浴霜  陈冬艳  张红卫 《遗传》2009,31(12):1233-1240
Hedgehog信号通路在胚胎发育过程中发挥着重要作用, 同时与多种肿瘤的发生密切相关。Rab23蛋白在Hedgehog信号通路中扮演着十分重要的角色。目前关于文昌鱼Rab23同源基因的研究仅局限于佛罗里达文昌鱼(Branchiostoma floridae)基因组中的注释。文章首次克隆了中国文昌鱼(Branchiostoma belcheri) Rab23b基因 (AmphiRab23b)cDNA全序列 , 对其演译的蛋白序列进行了序列比对、进化树分析以及基因时空表达分析。研究结果显示, 文昌鱼AmphiRab23b基因的 cDNA总长为2 062 bp (包括UTR区), 其中开放阅读框 (Open reading frame, ORF) 714 bp, 编码237个氨基酸; 虽然在进化树中不属于脊椎动物Rab23进化支, 但是AmphiRab23具有保守的Rab23_lke结构域, 暗示该基因在进化过程中可能在功能上是保守的。时空表达的研究结果进一步显示, AmphiRab23b基因在胚胎发育中的神经板和消化道中表达, 与其脊椎动物同源基因的表达模式相似, 这说明该基因可能对文昌鱼神经系统和消化道的发育有重要作用。  相似文献   

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Bone morphogenetic proteins (BMPs) are responsible for regulating embryo development and tissue homeostasis beyond osteogenesis. However, the precise biological roles of BMP3 and BMP3b remain obscure to a certain extent. In the present study, we cloned an orthologous gene (AmphiBMP3/3b) from amphioxus (Branchiostoma japonicum) and found its exon/intron organization is highly conserved. Further, in situ hybridization revealed that the gene was strongly expressed in the dorsal neural plate of the embryos. The gene also appeared in Hatschek’s left diverticulum, neural tube, preoral ciliated pit and gill slit of larvae, and adult tissues including ovary, neural tube and notochordal sheath. Additionally, real‐time quantitative polymerase chain reaction (RTqPCR) analysis revealed that the expression displayed two peaks at gastrula and juvenile stages. These results indicated that AmphiBMP3/3b, a sole orthologue of vertebrate BMP3 and BMP3b, might antagonize ventralizing BMP2 orthologous signaling in embryonic development, play a role in the evolutionary precursors of adenohypophysis, as well as act in female ovary physiology in adult.  相似文献   

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An amphioxus full-length cDNA, AmphiCalbin, encoding a novel EF-hand calcium-binding protein (EFCaBP), was isolated from the gut cDNA library of amphioxus Branchiostoma belcheri. It consists of 1321 bp with a 636 bp open reading frame encoding a protein of 211 amino acids with a molecular mass of approximately 24.5 kDa. The phylogenetic analysis offers two interesting inferences. First, AmphiCalbin clusters with a group of unnamed EFCaBPs that are differentiated from other identified EFCaBPs. Second, AmphiCalbin falls at the base of the vertebrate unnamed EFCaBPs clade, probably representing their prototype. This is also corroborated by the fact that AmphiCalbin has an exon-intron organization identical to that of vertebrate unnamed EFCaBP genes. Both tissue-section in situ hybridization and whole-mount in situ hybridization prove a tissue-specific expression pattern of AmphiCalbin, with high levels of expression in the digestive system and gonads. It is proposed that AmphiCalbin might play a role in the digestive system and gonads. These observations lay the foundation for further understanding of the function of the unnamed EFCaBPs.  相似文献   

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我国文昌鱼研究50年   总被引:1,自引:0,他引:1  
文昌鱼是研究脊椎动物起源与进化十分珍贵的模式动物。近50年来,我国在文昌鱼生物学研究方面取得很大进展。本文系统介绍了我国学者在文昌鱼发育、免疫和进化方面所取得的研究成果。  相似文献   

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A full-length amphioxus (Branchiostoma belcheri tsingtauense) NM23-Bbt2, NM23-H2 homologue, cDNA was isolated from the cDNA library and sequenced. The obtained amphioxus NM23-Bbt2 cDNA contains an open reading frame coding for 171 amino acids. Sequence analysis showed that the amphioxus NM23-Bbt2 was highly conserved with that of other species, and all of them contained highly conserved motifs that play important roles in the function of NM23. RT-PCR revealed that NM23-Bbt2 is expressed in the neuronal tissues and is expressed in all stages during the embryogenesis. Nucleoside kinases are thought to have a critical role in regulatory processes such as signal transduction, proliferation, and differentiation. Taken together, these results suggest that nucleoside diphosphate kinases have an important role to play in embryogenic development in amphioxus. Phylogenetic analysis showed that the amphioxus group 1 NDPKs (Bf1-4) may be precursors of the human group 1 NDPKs, NM23-Bf5, NM23-Bf6, NM23-Bf7 and NM23-Bf8 may be precursors of NM23-H5, NM23-H6, NM23-H7 and M23-H8, respectively. Our finding of nine NM23 genes in Branchiostoma floride, the precursor of vertebrates, strongly suggests that the ancestral gene corresponding to each of vertebrates NM23 genes generated before the appearance of vertebrates. Comparison of the gene structures of NM23-H2 homologue from invertebrates to vertebrates suggests that the locations of three of the four introns are conserved in amphioxus and vertebrates.  相似文献   

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Y. Zhang    L. Wang    M. Shao    J. Li    B. Li  † H. Zhang   《Journal of fish biology》2008,72(5):1215-1222
An orthologue of hadh2 (hydroxyacyl-coenzyme A dehydrogenase type II) has been isolated from amphioxus. At the amino acid level, hadh2 exhibits high sequence similarity between amphioxus and vertebrates, including zebrafish. Similarities also exist in the developmental expression patterns of amphioxus and zebrafish hadh2 , which may provide information on the molecular mechanisms responsible for some human disease phenotypes caused by hadh2 mutation.  相似文献   

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The expression of four AmphiPax genes in 16 developmental stages and different organs in amphioxus (Branchiostoma belcheri) was investigated, finding those genes expressed throughout amphioxus life with temporal-specific (especially during embryogenesis and metamorphosis) and spatial-specific patterns. This study suggests that duplicated Pax genes in vertebrates might maintain most of their ancestral functions and also expand their expression patterns after the divergence of protochordates and vertebrates.  相似文献   

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Yu Y  Yuan S  Yu Y  Huang H  Feng K  Pan M  Huang S  Dong M  Chen S  Xu A 《Glycobiology》2007,17(7):774-783
A novel F4-carbohydrate recognition domain (CRD)-linker-F3-CRD-type bi-CRD Branchiostoma belcheri tsingtauense galectin (BbtGal)-L together with its alternatively spliced mono-CRD isoform BbtGal-S from amphioxus intestine was encoded by a 9488-bp unique gene with eight exons and seven introns. The recombinant proteins of BbtGal were found to have beta-galactoside-binding activity, indicating that BbtGal was a member of the galectin family. Phylogenetic analysis of this gene along with its splicing form and genome structure suggested that the BbtGal gene was the primitive form of the chordate galectin family. Real-time polymerase chain reaction analyses (PCR) indicated that BbtGal mRNA was expressed during all stages of embryonic development. In terms of tissue distribution, BbtGal-L mRNA was mainly expressed in the immunity-related organs, such as hepatic diverticulum, intestine, and gill, but BbtGal-S was ubiquitously expressed in all tissues. The expression of BbtGal-L mRNA was elevated after acute challenge with various microorganisms, but BbtGal-L only bound to specific bacteria. The immune function of BbtGal was consistent with its localization both outside and inside the cell. Our study on amphioxus galectin may help further understanding of the evolution of chordate galectin in terms of host-pathogen interaction in the immune system.  相似文献   

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