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1.
The effect of methyl jasmonate (JA-Me) applied in concentration 1.0 % in lanolin paste to detached tomato fruits at the mature
green, advanced mature green and light red stages on the ethylene production and l-aminocyclopropane-l-carboxylic acid (ACC)
content was investigated at different times after treatment. JA-Me stimulated ethylene production in all stages of ripening,
but the level of ACC increased or decreased in comparison with control depending on the stage of ripening. Higher level of
ACC in JA-Me treated tissue was found in mature green stage and fully ripened tomatoes-treated at advanced green stage; lower
one in light red stage — treated at advanced green stage and fully ripened stage - treated at light red stage. 相似文献
2.
Aminooxyacetic acid (AOA), an inhibitor of ACC biosynthesis, applied together with methyl jasmonate (JA-Me) to mature green
and light-green tomatoes cv. Venture greatly inhibited ethylene production stimulated by JA-Me, when analyzed in ripe and
overripe stages. AOA applied alone did not affect ethylene production in the same conditions of treatment and analysis. It
is suggested that after JA-Me treatment of tomatoes the turnover rate of ACC is higher (JA-Me stimulates EFE activity) in
comparison to control tissues, and, consequently, AOA inhibited ethylene production stimulated by methyl jasmonate. 相似文献
3.
Raad Mansour Alain Latché Virginie Vaillant Jean-Claude Pech Michael S. Reid 《Physiologia plantarum》1986,66(3):495-502
In preclimacteric apple fruits ( Malus × domestica Borkh. cv. Golden Delicious) ethylene production is controlled by the rates of 1-aminocyclopropane-1-carboxylic acid (ACC) synthesis, and by its metabolism to ethylene by the ethylene-forming enzyme and to 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC) by malonyl CoA-ACC transferase. The onset of the climacteric in ethylene production is associated with an increase in the activity of the ethylene-forming enzyme in the pulp and with a rise in the activity of ACC synthase. Malonyl transferase activity is very high in the skin of immature fruit, decreases sharply before the onset of the climacteric, and remains nearly constant thereafter. More than 40% of the ACC synthesized in the skin and around 5% in the flesh, are diverted to MACC at early climacteric. At the climacteric peak there are substantial gradients in ethylene production between different portions of the tissue, the inner cortical tissues producing up to twice as much as the external tissues. This increased production is associated with, and apparently due to, increased content of ACC synthase. Less than 1% of the synthesized ACC is diverted to MACC in the flesh of climacteric apples. In contrast, the skin contains high activity of malonyl transferase, and correspondingly high levels [1000 nmol (g dry weight)−1 ] of MACC. 相似文献
4.
Tomato fruits on stems immersed in phosphate solution 0.2 M K2HPO4 produced less ethylene than control fruits on stems immersed in water. Phosphate mediated inhibition of ethylene production
was found to be the highest in fruits in the pink stage of maturity, which produced ethylene at the highest rate. Phosphate
also inhibited ethylene production in slices prepared from maturing fruits, both apple and tomato. We suggest that phosphate
is an inhibitor of ethylene biosynthesis in sufficiently mature tomato and apple fruits in which the rise of ethylene production
is already very rapid.
Presented at the International Symposium “Plant Growth Regulators” held on June 18–22, 1984 at Liblice, Czechoslovakia. 相似文献
5.
Li Zhen-guo Liu Yu Dong Jian-guo Xu Rong-jiang Zhu Mei-zhen 《Journal of Plant Growth Regulation》1983,2(1-4):81-87
The association of the level of ACC and the ethylene concentration in ripening apple fruit (Malus sylvestris Mill, var. Ben Davis) was studied. Preclimacteric apple contained small amounts of ACC and ethylene. With the onset of the climacteric and a concomitant decrease in flesh firmness, the level of ACC and ethylene concentration both increased markedly. During the postclimacteric period, ethylene concentration started to decline, but the level of ACC continued to increase. Ethylene production and loss of flesh firmness of fruits during ripening were greatly suppressed by treatments with low O2 (O2 1–3%, CO2 O%) or high CO2 (CO2 20–30%, O2 15–20%) at the preclimacteric stage. However, after 4 weeks an accumulation of ACC was observed in treated fruits when control fruit was at the postclimacteric stage. Treatment of fruit with either low O2 or high CO2 at the climacteric stage resulted in a decrease of ethylene production. However, the ACC level in fruit treated with low O2 was much higher than both control and high CO2 treated fruit; it appears that low O2 inhibits only the conversion of ACC to ethylene, resulting in an accumulation of ACC. Since CO2 inhibits ethylene production but does not result in an accumulation of ACC, it appears that high CO2 inhibits both the conversion of ACC to ethylene and the formation of ACC. 相似文献
6.
The effect of light on the production of ethylene from 1-aminocyclopropane-1-carboxylic acid by leaves 总被引:1,自引:0,他引:1
White light inhibits the conversion of 1-amino-cyclopropane-1-carboxylic acid (ACC) in discs of green leaves of tobacco (Nicotiana tabacum L.) and segments of oat (Avena sativa L.) leaves by from 60 to 90%. Etiolated oat leaves do not show this effect. The general nature of the effect is shown by its presence in both a mono- and a dicotyledon. Since the leaves have been grown and pre-incubated in light, yet can produce from 2 to 9 times as much ethylene in the dark as in the light, it follows that the light inhibition is fully reversible. The inhibition by light is about equal to that exerted in the dark by CoCl2; it can be partly reversed by dithiothreitol and completely by mercaptoethanol. Thus the light is probably acting, via the photosynthetic system, on the SH group(s) of the enzyme system converting ACC to ethylene.Abbreviation ACC
1-aminocyclopropane-1-carboxylic acid 相似文献
7.
Excised wheat (Triticum aestivum L.) leaves, when subjected to drought stress, increased ethylene production as a result of an increased synthesis of 1-aminocyclopropane-1-carboxylic acid (ACC) and an increased activity of the ethyleneforming enzyme (EFE), which catalyzes the conversion of ACC to ethylene. The rise in EFE activity was maximal within 2 h after the stress period, while rehydration to relieve water stress reduced EFE activity within 3 h to levels similar to those in nonstressed tissue. Pretreatment of the leaves with benzyladenine or indole-3-acetic acid prior to water stress caused further increase in ethylene production and in endogenous ACC level. Conversely, pretreatment of wheat leaves with abscisic acid reduced ethylene production to levels produced by nonstressed leaves; this reduction in ethylene production was accompanied by a decrease in ACC content. However, none of these hormone pretreatments significantly affected the EFE level in stressed or nonstressed leaves. These data indicate that the plant hormones participate in regulation of water-stress ethylene production primarily by modulating the level of ACC.Abbreviations ABA
abscisic acid
- ACC
1-aminocyclopropane-1-carboxylic acid
- BA
N6-benzyladenine
- EFE
ethylene-forming enzyme
- IAA
indole-3-acetic acid 相似文献
8.
The conversion of 1-aminocyclopropane 1-carboxylic acid (ACC) to ethylene by hypocotyl segments of sunflower (Helianthus annuus L.) seedlings was inhibited by abscisic acid (ABA) and methyl jasmonate (Me-Ja), and this inhibitory effect increased with increasing concentration of both growth regulators. On the contrary, CaCl, enhanced ACC conversion to ethylene at the concentrations of 10-4 M and 5 x 10-4 M, however lower and higher concentrations had no significant action. CaCl, (5 x 10-4M) seemed to magnify the inhibition of the reaction induced by ABA, whereas it reduced (5 x 10-4M) and even abolished (10-3M) the inhibitory action of Me-Ja. The results obtained with a Ca2+ chelator (EGTA), a Ca2+ channel blocker (nifedipine) and calmodulin antagonists (W7 and TFP), given in association with ABA or Me-Ja, suggested that calcium was involved in the inhibition of ACC conversion to ethylene by ABA and Me-Ja through an interaction with calmodulin. However, the mechanism of action of the two growth regulators seemed to be different, since all treatments which resulted in a decrease in cytosolic Ca2+ concentration or in calmodulin action induced a decrease in the effect of ABA and an increase in the effect of Me-Ja.Abbreviations ABA
abscisic acid
- ACC
1-aminocyclopropane 1-carboxylic acid
- EFE
ethylene for enzyme
- EGTA
ethylene glycol-bis-2-aminoethyl tetraacetic acid
- Me-Ja
methyl jasmonate
- NIF
nifedipine
- TFP
trifluoperazine dihydrochloride
- W7
N-(6-aminohexyl)5-chloro-l-naphthalenesulfonamide hydrochloride 相似文献
9.
The effect of methyl jasmonate (JA-Me), applied to mature green tomato fruits cv. Modena, on the content of some fatty acids in ripe fruits was studied. Methyl jasmonate greatly increased content of linolenic acid and in the lesser degree decreased the amount of linoleic acid. The ratio of linolenic acid to linoleic acid content increased 4.5–7.7 times in methyl jasmonate treated samples in comparison to untreated-controls. JA-Me did not affect the contents of lauric, myristic, palmitic, stearic, palmitoleic and oleic acids. 相似文献
10.
Eve Dupille César Rombaldi Jean-Marc Lelièvre Jean-Claude Cleyet-Marel Jean-Claude Pech Alain Latché 《Planta》1993,190(1):65-70
The enzyme which converts 1-aminocyclo-propane-1-carboxylic acid (ACC) into ethylene, ACC oxidase, has been isolated from apple fruits (Malus x domestica Borkh. cv. Golden Delicious), and for the first time stabilized in vitro by 1,10-phenanthroline and purified 170-fold to homogeneity in a five-step procedure. The sodium dodecyl sulfate-denatured and native proteins have similar molecular weights (approx. 40 kDa) indicating that the enzyme is active in its monomeric form. Antibodies raised against a recombinant ACC oxidase over-produced in Escherichia coli from a tomato cDNA recognise the apple-fruit enzyme with high specificity in both crude extracts and purified form. Glycosylation appears to be absent because of (i) the lack of reactivity towards a mixture of seven different biotinylated lectins and (ii) the absence of N-linked substitution at a potential glycosylation site, in a sequenced peptide. Phenylhydrazine and 2-methyl-1-2-dipyridyl propane do not inhibit activity, indicating that ACC oxidase is not a prosthetic-heme iron protein. The partial amino-acid sequence of the native protein has strong homology to the predicted protein of a tomato fruit cDNA demonstrated to encode ACC oxidase. 相似文献
11.
R. Nichols G. Bufler Y. Mor D. W. Fujino M. S. Reid 《Journal of Plant Growth Regulation》1983,2(1-4):1-8
Pollination of flowers of standard carnation (Dianthus caryophyllus L. cv. White Sim) with pollen from flowers of miniature carnations (D. caryophyllus L. cv. Exquisite) caused them to wilt irreversibly within 1 to 2 days. Pollination stimulated a sequential increase in ethylene production by stigmas, ovaries, receptacles, and petals of the flowers. The ACC content of the stigmas increased rapidly in the first few hours after pollination. The possibility that subsequent production of ethylene by other parts of the flower is stimulated by translocated ACC is discussed. Ethylene production and ACC content of other parts of the flower reached their maximum 24 h after pollination. The petal tissues contributed the bulk of the ethylene productionper flower thereafter. There appears to be a qualitative difference between the enzyme in the stigmas converting ACC to ethylene and that in other parts of the flower. 相似文献
12.
Calcium effects on ethylene and ethane production and 1-aminocyclopropane-1-carboxylic acid content in potato disks 总被引:2,自引:0,他引:2
Kathleen B. Evensen 《Physiologia plantarum》1984,60(2):125-128
Potato ( Solanum tuberosum L. cv. Katahdin) disks produce ethyline in increasing amounts from 6 to 24 h incubation in buffer at pH 4.0. Ethylene production is increased 2–3 times in the presence of 50 m M CaCl2 . Levels of endogenous 1-amino-cyclopropane-1-carboxylic acid (ACC) increase in parallel with ethylene production, and ACC levels are 3–5 times higher in calcium-treated disks than in controls. Most of the calcium-induced stimulation of ethylene production can be accounted for by its effect on ACC production, indicating that the primary effect of calcium is on a step of ethylene biosynthesis preceeding ACC production. However, calcium may also have an effect on conversion of ACC to ethylene, since a consistent increase in ACC-de-pendent ethylene production was observed in the presence of calcium. Production of ethane, a marker of lipid peroxidation, was reduced by calcium, so it is possible that membrane stabilization by calcium could be involved in its effects on ethylene production. 相似文献
13.
At harvest, fruit from apple trees sprayed with daminozide (+daminozide) had lower levels of aminocyclopropane-1-carboxylic acid (ACC) and produced significantly lower amounts of ethylene than untreated (–daminozide) fruit. Flesh discs from the fruit of +daminozide and –daminozide trees were fed precursors of ethylene to determine how daminozide inhibits ethylene production. ACC was metabolized to ethylene regardless of treatment. Methionine (MET), however, was only converted to ethylene by –daminozide fruit, and only after the fruit had been maintained at 4 °C for 5 months. +Daminozide fruit failed to convert MET to ethylene at harvest, as well as after cold storage. When daminozide was added to the incubation media of flesh discs it did not inhibit ethylene production or the conversion of ACC to ethylene. The addition of daminozide did, however, inhibit the metabolism of exogenous MET to ethylene. Aminooxyacetate acid (AOA) blocked both the endogenous production of ethylene and that from MET feeds. Daminozide inhibits ethylene production by preventing the conversion of MET to ACC, but it does not appear to act as a simple competitive inhibitor of ACC synthase activity.Abbreviations ACC
aminocyclopropane-1-carboxylic acid
- AVG
aminoethoxyvinylglycine
- AOA
aminooxyacetic acid
- CH
cycloheximide
- MET
methionine
- PUT
putrescine
Author for correspondence 相似文献
14.
Journal of Plant Growth Regulation - Ca2+ stimulates 1-aminocyclopropane-1-carboxylic acid (ACC)- and indole-3-acetic acid (IAA)-dependent ethylene production in mung bean hypocotyls and senescing... 相似文献
15.
16.
Methyl jasmonate (JA-Me) at a concentration of 0.5% in lanolin paste totally inhibited bulblets formation induced by benzyladenine
in intactMuscari bulbs. Lower concentrations of JA-Me delayed development and growth of bulblets induced by benzyladenine. It seems that methyl
jasmonate acts as a powerful inhibitor of cell division induced by cytokinin in used test. In comparison with methyl jasmonate,
abscisic acid did not show an inhibitory effect on bulblets formation induced by benzyladenine, even in a higher concentration. 相似文献
17.
Synergistic effect of 1-aminocyclopropane-1-carboxylic acid and ethylene during senescence of isolated carnation petals 总被引:2,自引:0,他引:2
The effects of ethylene (C2 H4 ), (2-chloroethyl)phosphonic acid (ethefon) and 1-aminocyclopropane-1-carboxylic acid (ACC) on senescence of isolated intact petals and of upper petal parts of carnation flowers ( Dianthus caryophyllus L. cv. White Sim) were investigated.
Isolated upper petal parts did not respond to treatment with ethefon or ACC. These tissues did, however, show severe wilting in intact petals that were treated with ethefon or ACC. When isolated upper petal parts were simultaneously treated with ACC and ethefon or ACC and ethylene, a marked synergistic effect on senescence was found. Treatment of isolated petals with radiolabeled ACC led to the accumulation of radiolabeled ACC and N-malonyl-ACC (MACC) in the upper parts. The formation of ethylene and the malonylation of ACC were inhibited by pretreatment of the flower with the inhibitor of ethylene action, silver thiosulphate (STS), which indicates that both were induced by endogenously produced ethylene. Treatment of isolated upper parts with ACC slightly increased their ethylene production. However, when these petal parts were simultaneously treated with ethylene and ACC, the conversion of ACC to ethylene was markedly stimulated.
The results indicate that, in intact petals, ethylene may be translocated from the basal to the upper part where it stimulates the activity of the ethylene-forming enzyme (EFE), thereby making the tissue receptive to ACC.
In addition, it was found that upon incubation of petal portions in radiolabeled ACC, both the petal tissue and the incubation solutions produced radiolabeled carbon dioxide. This was shown to be due to microorganisms that were able to metabolize the carbon atoms in the 2 and 3 position of ACC into carbon dioxide. 相似文献
Isolated upper petal parts did not respond to treatment with ethefon or ACC. These tissues did, however, show severe wilting in intact petals that were treated with ethefon or ACC. When isolated upper petal parts were simultaneously treated with ACC and ethefon or ACC and ethylene, a marked synergistic effect on senescence was found. Treatment of isolated petals with radiolabeled ACC led to the accumulation of radiolabeled ACC and N-malonyl-ACC (MACC) in the upper parts. The formation of ethylene and the malonylation of ACC were inhibited by pretreatment of the flower with the inhibitor of ethylene action, silver thiosulphate (STS), which indicates that both were induced by endogenously produced ethylene. Treatment of isolated upper parts with ACC slightly increased their ethylene production. However, when these petal parts were simultaneously treated with ethylene and ACC, the conversion of ACC to ethylene was markedly stimulated.
The results indicate that, in intact petals, ethylene may be translocated from the basal to the upper part where it stimulates the activity of the ethylene-forming enzyme (EFE), thereby making the tissue receptive to ACC.
In addition, it was found that upon incubation of petal portions in radiolabeled ACC, both the petal tissue and the incubation solutions produced radiolabeled carbon dioxide. This was shown to be due to microorganisms that were able to metabolize the carbon atoms in the 2 and 3 position of ACC into carbon dioxide. 相似文献
18.
1-Aminocyclopropane-1-carboxylic acid (ACC) oxidase catalyzes the oxidation of ACC to the gaseous plant hormone, ethylene. Although the enzyme does not contain a typical N-terminal consensus sequence for the transportation across the endoplasmic reticulum (ER), it has recently been shown to locate extracellularly by immunolocalization study. It was of interest to examine whether the enzyme contains a signal peptide that is overlooked by structure prediction. We observed that the in vitro translated apple ACC oxidase was not co-processed or imported by the canine pancreatic rough microsomes, a system widely used to identify signal peptide for protein translocation across ER, suggesting that apple ACC oxidase does not contain a signal peptide for ER transport. A highly specific polyclonal antibody raised against the recombinant apple ACC oxidase was used to examine the subcellular localization of the enzyme in apple fruit (Malus domestica, var. Golden Delicious). The location of ACC oxidase appeared to be mainly in the cytosol of the apple fruit pericarp tissue as was demonstrated by electron microscopy using immunogold-labeled antibodies. The pre-immune serum or pre-climacteric fruit control gave essentially no positive signal. Based on these observations, we conclude that ACC oxidase is a cytosolic protein. 相似文献
19.
Cyclopropane-1,1-dicarboxylic acid (CDA) and trans-2-phenylcyclopropane-1-carboxylic acid (PCCA) are the main representatives of a group of compounds that are structural analogues of 1-aminocyclopropane-1-carboxylic acid (ACC) and have been proved to have an inhibitory effect on the wound ethylene produced by Lycopersicum esculentum fruit discs. During the experiments, that were carried out in this work the inhibition pattern of PCCA and CDA were studied when tested on partially purified apple ACO and their Ki values were determined. A mechanistic proposal was given, in order to explain the kinetic behaviour of the inhibitors. The common feature of these molecules is their cyclopropane ring, with different substitutes mainly at the positions C1 and C2. Two other compounds with similar structure where also tested as inhibitors, in order to clarify the relationship between structure and activity. These compounds are: 2-methyl cyclopropanecarboxylic acid (MCA), and cyclopropanecarboxylic acid (CCA). 相似文献
20.
Immunocytolocalization of 1-aminocyclopropane-1-carboxylic acid oxidase in tomato and apple fruit 总被引:4,自引:0,他引:4
Cesar Rombaldi Jean-Marc Lelièvre Alain Latché Michel Petitprez Mondher Bouzayen Jean-Claude Pech 《Planta》1994,192(4):453-460
The subcellular localization of 1-aminocyclopropane-1-carboxylic acid oxidase (ACC oxidase), an enzyme involved in the biosynthesis of ethylene, has been studied in ripening fruits of tomato (Lycopersicum esculentum Mill.). Two types of antibody have been raised against (i) a synthetic peptide derived from the reconstructed pTOM13 clone (pRC13), a tomato cDNA encoding ACC oxidase, and considered as a suitable epitope by secondary-structure predictions; and (ii) a fusion protein overproduced in Escherichia coli expressing the pRC13 cDNA. Immunoblot analysis showed that, when purified by antigen affinity chromatography, both types of antibody recognized a single band corresponding to ACC oxidase. Superimposition of Calcofluor white with immunofluorescence labeling, analysed by optical microscopy, indicated that ACC oxidase is located at the cell wall in the pericarp of breaker tomato and climacteric apple (Malus × domestica Borkh.) fruit. The apoplasmic location of the enzyme was also demonstrated by the observation of immunogold-labeled antibodies in this region by both optical and electron microscopy. Transgenic tomato fruits in which ACC-oxidase gene expression was inhibited by an antisense gene exhibited a considerable reduction of labeling. Immunocytological controls made with pre-immune serum or with antibodies pre-absorbed on their corresponding antigens gave no staining. The discrepancy between these findings and the targeting of the protein predicted from sequences of ACC-oxidase cDNA clones isolated so far is discussed. 相似文献