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1.
Modulatory Action of Taurine on the Release of GABA in Cerebellar Slices of the Guinea Pig 总被引:6,自引:4,他引:2
Abstract: For the purpose of demonstrating the action of taurine as a neuromodulator in addition to its suggested neurotransmitter function, the effects of taurine and muscimol on the depolarization-induced Ca-dependent release of [3 H]γ-aminobutyric acid (pH]GABA) and l -[3 H]glutamate in cerebellar slices from guinea pigs were investigated. The release of [3 H]GABA was found to be greatly decreased by a GABA agonist, muscimol, and by taurine, but not by glycine. The release of l -[3 H]glutamate was little affected by taurine. The release of [3 H]GABA was enhanced by bicuculline and strychnine, but not by picrotoxin, and the suppressive action of muscimol on the GABA release was antagonized by bicuculline, picrotoxin, and strychnine, suggesting the possible existence of presynaptic autoreceptors for GABA in the cerebellum. The suppressive action of taurine on the release of [3 H]GABA, on the other hand, was blocked only by bicuculline. These results suggest that taurine reduced the release of [3 H]GABA from cerebellar slices by acting on the GABA autoreceptors or, more likely, on other types of receptors that are sensitive to bicuculline. As a possible mechanism for this modulatory action of taurine, the blockade by this amino acid of the influx of Ca2+ into cerebellar tissues was tentatively suggested. 相似文献
2.
Hoau-Yan Wang †Tian-Li Yue †‡Giora Feuerstein ‡ Eitan Friedman 《Journal of neurochemistry》1994,63(5):1720-1725
Abstract: The effect of platelet-activating factor (PAF) on neurotransmitter release from rat brain slices prelabeled with [3 H]acetylcholine ([3 H]ACh), [3 H]norepinephrine ([3 H]NE), or [3 H]serotonin ([3 H]5-HT) was studied. PAF inhibited K+ depolarization-induced [3 H]ACh release in slices of brain cortex and hippocampus by up to 59% at 10 n M but did not inhibit [3 H]ACh release in striatal slices. PAF did not affect 5-HT or NE release from cortical brain slices. The inhibition of K+ -evoked [3 H]ACh release induced by PAF was prevented by pretreating tissues with several structurally different PAF receptor antagonists. The effect of PAF was reversible and was not affected by pretreating brain slices with tetrodotoxin. PAF-induced inhibition of [3 H]ACh release was blocked 90 ± 3 and 86 ± 2% by pertussis toxin and by anti-Gαi1/2 antiserum incorporated into cortical synaptosomes, respectively. The results suggest that PAF inhibits depolarization-induced ACh release in brain slices via a Gαi1/2 protein-mediated action and that PAF may serve as a neuromodulator of brain cholinergic system. 相似文献
3.
Abstract: Slices of rabbit caudate and hypothalamus take up and accumulate [3 H]imipramine. In superfused slices of both structures electrical stimulation or exposure to tyramine failed to release recently taken up [3 H]imipramine. De-polarization by exposure to 30–60 mm-potassium caused only a small release of [3 H]imipramine that was not concentration-dependent. The release of [3 H]imipramine by high potassium was independent of the presence of calcium ions in the superfusion medium. These results contrasted with those obtained for the release of [3 H]dopamine from the caudate and [3 H]noradrenaline from the hypothalamus, where tyramine, electrical stimulation, and high potassium caused a significant release of the labeled neurotransmitters. The release of [3 H]dopamine from the caudate and [3 H]noradrenaline from the hypothalamus elicited by electrical stimulation or high potassium was entirely calcium-dependent. It is concluded that [3 H]imipramine is taken up into the two brain regions and is accumulated in a nonvesicular site from which it is not released by calcium-dependent depolarizing stimuli. 相似文献
4.
Effects of Arachidonic Acid on Dopamine Synthesis, Spontaneous Release, and Uptake in Striatal Synaptosomes from the Rat 总被引:1,自引:0,他引:1
Abstract: Arachidonic acid (AA) markedly stimulated, in a dose-dependent manner, the spontaneous release of [3 H]dopamine ([3 H]DA) continuously synthesized from [3 H]tyrosine in purified synaptosomes from the rat striatum. As estimated by simultaneous measurement of the rate of [3 H]H2 O formation (an index of [3 H]tyrosine conversion into [3 H]DOPA), the AA response was associated with a progressive and dose-dependent reduction of [3 H]DA synthesis. In contrast to AA, arachidic acid, oleic acid, and the methyl ester of AA (all at 10−4 M ) did not modify [3 H]DA release. The AA (3 × 10−5 M )-evoked release of [3 H]DA was not affected by inhibiting AA metabolism, with either 5,8,11,14-eicosatetraynoic acid or metyrapone, suggesting that AA acts directly and not through one of its metabolites. AA also inhibited in a dose-dependent manner [3 H]DA uptake into synaptosomes, with a complete blockade observed at 10−4 M . However, AA (10−4 M ) still stimulated [3 H]DA spontaneous release in the presence of either nomifensine or other DA uptake inhibitors, indicating that AA both inhibits DA reuptake and facilitates its release process. Finally, the AA (10−4 M )-evoked release of [3 H]DA was not affected by protein kinase A inhibitors (H-89 or Rp -8-Br-cAMPS) but was markedly reduced in the presence of protein kinase C inhibitors (Ro 31-7549 or chelerythrine). 相似文献
5.
Seitaro Ohkuma Hidehiko Narihara Masashi Katsura Takeshi Hasegawa Kinya Kuriyama 《Journal of neurochemistry》1995,65(3):1109-1114
Abstract: The functional significance of peroxynitrite in the release of [3 H]GABA induced by nitric oxide (NO) liberated from NO generators was investigated using cerebral cortical neurons in primary culture. NO generators such as sodium nitroprusside (SNP) and S -nitroso- N -acetylpenicillamine (SNAP) increased [3 H]GABA release in a dose-dependent manner. These increases in [3 H]GABA release were significantly inhibited by hemoglobin, indicating that those NO generators evoke the release of [3 H]GABA by the formation of NO. Two types of superoxide scavengers, Cu2+ /Zn2+ superoxide dismutase and ceruloplasmin, significantly reduced the increase in [3 H]GABA release induced by both SNP and SNAP, which assumes that NO requires superoxide to induce [3 H]GABA release from the neurons. In addition, synthesized peroxynitrite induced a dose-dependent increase in [3 H]GABA release from the neurons. These results indicate that NO-induced [3 H]GABA release is mediated by peroxynitrite formed by the reaction of NO with superoxide. 相似文献
6.
The Effect of Colchicine and Cytochalasin B on the Release of Taurine from the Chick Retina 总被引:1,自引:1,他引:0
Abstract: The effect of colchicine (0.5 mM) and of cytochalasin B (10−4 M) on the release of [35 S]taurine from the isolated chick retina, upon stimulation by 68.5 mM-KCl, 10−5 M-veratridine and 10 mM-glutamate, was studied. Cytochalasin and colchicine effects on taurine release were compared with those on K+ -stimulated release of [3 H]dopamine and [3 H]GABA. Colchicine caused a marked decrease of the [35 S]taurine release evoked by the three stimulatory agents; it also decreased [3 H]dopamine release without affecting that of [3 H]GABA. Cytochalasin B significantly decreased the efflux of [35 S]taurine stimulated by glutamate or veratridine without altering that evoked by 68.5 mM-KCl. Cytochalasin practically suppressed the [3 H]dopamine-stimulated release and slightly decreased that of [3 H]GABA. This drug produced a high increase in the spontaneous release of labeled GABA and taurine. These results suggest that the release of taurine and GABA from the chick retina probably occurs through different mechanisms. It is suggested that taurine release may be related to a process involving contractile proteins. 相似文献
7.
Shiaw-Der Yang Jau-Song Yu Shine-Gwo Shiah Jun-Jae Huang 《Journal of neurochemistry》1994,63(4):1416-1425
In this work, we tested the effect of ion channel blockers and of phorbol ester treatments on [3 H]dopamine ([3 H]DA) release and neurotensin (NT)-induced facilitation of [3 H]DA release from cultures of rat fetal mesencephalic cells. The potassium channel blockers tetraethylammonium and 4-aminopyridine increased basal [3 H]DA release and decreased K+ -evoked [3 H]DA release, whereas apamin was without effect. K+ -evoked [3 H]DA release was decreased by ω-conotoxin and nifedipine, totally suppressed by cadmium, and unaffected by amiloride. These results show the differential sensitivity of [3 H]DA release to blockade of various ion channels and suggest the involvement of N-type, L-type, and non-L-non-N-type, but not T-type, voltage-sensitive calcium channels in K+ -evoked release. Phorbol 12-myristate 13-acetate increased both spontaneous and K+ -evoked [3 H]DA release, suggesting a modulatory action of protein kinase C on DA release in this system. Unexpectedly, however, the effects of the phorbol ester were not counteracted by the protein kinase C inhibitors H7, staurosporine, or polymyxin B. NT-induced facilitation of K+ -evoked [3 H]DA release was insensitive to most of the ion channel blockers, except cadmium (64% decrease in NT effect), suggesting that the corresponding potassium' and calcium channels were not involved in the effect of NT on [3 H]DA release in this system. The NT effect was totally suppressed by phorbol ester treatments, indicating a possible desensitization of the corresponding transduction mechanisms after protein kinase C activation. 相似文献
8.
Potassium-Stimulated Release of Radiolabelled Taurine and Glycine from the Isolated Rat Retina 总被引:4,自引:3,他引:1
Abstract: The release of preloaded [3 H]glycine and [3 H]taurine in response to a depolarising stimulus (12.5-50 m M KCl) has been studied in the superfused rat retina. High external potassium concentration immediately increased the spontaneous efflux of [3H]glycine, the effect of 50 m M K+ apparently being abolished by omitting calcium from the superfusing medium. In contrast, although high potassium concentrations increased the spontaneous emux of [3 H]taurine from the superfused rat retina, this release was not evident until the depolarising stimulus was removed from the superfusing medium. The magnitude of this "late" release of [3 H]taurine was dependent on external K+ concentrations, and appeared immediately after cessation of the stimulus irrespective of whether it was applied for 4, 8, or 12 min. Potassium (50 m M )-induced release of taurine appeared partially calcium-dependent, being significantly reduced (p < 0.01) but not abolished by replacing calcium with 1 mM EDTA in the superfusate. High-affinity uptake systems for both [3 H]glycine and [3 H]taurine were demonstrated in the rat retina in vitro ( K m values, 1.67 μ M and 2.97 μ M ; Vmax values, 19.3 and 23.1 nmol/g wet weight tissue/h, respectively). The results are discussed with respect to the possible neuro-transmitter roles of both amino acids in the rat retina. 相似文献
9.
THE EFFECT OF ELECTRICAL STIMULATION AND HIGH POTASSIUM CONCENTRATIONS ON THE EFFLUX OF [3 H] γ-AMINOBUTYRIC ACID FROM BRAIN SLICES 总被引:10,自引:0,他引:10
Abstract— Brain slices were incubated with [3 H]GABA in a medium containing aminooxyacetic acid to prevent metabolism of [3 H]GABA by GABA-glutamate transaminase. The slices, which rapidly accumulated radioactivity, were then continuously perfused and the efflux of [3 H]GABA from the tissue was measured. The spontaneous efflux of [3 H]GABA consisted of an initial rapid phase followed by a much slower release of [3 [H]GABA. After 40 min perfusion 90 per cent of the radioactivity remained in the tissue.
The slices were depolarized by electrical stimulation or by perfusion with a medium containing a high potassium concentration (40 mM). These procedures caused a striking increase in the efflux of [3 H]GABA. The increased efflux produced by potassium, but not that produced by electrical stimulation, was dependent on calcium ions in the medium. The effect of electrical stimulation on [3 H]GABA release was considerably reduced by a raised concentration (10 mM) of magnesium in the medium.
High potassium concentrations and electrical stimulation did not cause an increase in the efflux of [14 C]urea, L-[3 H]leucine or [14 C]α-amino-isobutyric acid from brain slices. These results are consistent with the suggestion that GABA may be an inhibitory transmitter in the cerebral cortex. 相似文献
The slices were depolarized by electrical stimulation or by perfusion with a medium containing a high potassium concentration (40 mM). These procedures caused a striking increase in the efflux of [
High potassium concentrations and electrical stimulation did not cause an increase in the efflux of [
10.
Abstract: Serotonin (5-hydroxytryptamine, 5-HT; 0.5 μM and above) stimulated the release of [3 H]dopamine ([3 H]DA) from particulate fractions of the carp ( Cyprinus carpio ) retina. The 5-HT effect was dose- and Ca2+ -dependent, and was structurally specific. A similar response was not elicited by the other indoles (5,6-dihydroxytryptamine, 5,7-dihydroxytryptamine, 5-hydroxytrypto-phan, or 5-hydroxyindoleacetic acid) examined. An increase in [3 H]DA release was elicited by addition of 5-HT agonists (5-methoxytryptamine, 5-methoxy- N,N- dimethyltryptamine, and tryptamine), but not antagonized by three 5-HT antagonists (metergolin, methysergide, and spiperone). Either DA alone or noradrenaline (0.5 m M ) produced a large increase in [3 H]DA release from the particulate fractions, but this action was Ca2+ -independent. Further, no significant release of [3 H]γ-aminobutyric acid could be evoked by 5-HT (0.5 mM) under similar experimental conditions. Taken together, the present data suggest that 5-HT stimulates [3 H]DA release from the fish retina through a specific receptor-mediated mechanism on dopaminergic terminals, but not through an exchange or nonspecific phenomenon. 相似文献
11.
Massimo Grilli Anna Pittaluga† Emilio Merlo-Pich‡ Mario Marchi† 《Journal of neurochemistry》2009,108(2):408-416
In this study, we investigate the effects of chronic administration of (−)nicotine on the function of the NMDA-mediated modulation of [3 H]dopamine (DA) release in rat prefrontal cortex (PFC) and nucleus accumbens (NAc). In the PFC synaptosomes NMDA in a concentration-dependent manner evoked [3 H]DA release in rats chronically treated with vehicle (14 days) with an EC50 of 13.1 ± 2.0 μM. The NMDA-evoked overflow of the [3 H]DA in PFC nerve endings of rats treated with (−)nicotine was significantly lower (−43%) than in vehicle treated rats. The EC50 was 9.0 ± 1.4 μM. Exposure of NAc synaptosomes of rats treated with vehicle to NMDA produced an increase in [3 H]DA overflow with an EC50 of 14.5 ± 5.5 μM. This effect was significantly enhanced in chronically treated animals. The EC50 was 10.5 ± 0.5 μM. The K+ -evoked release of [3 H]DA was not modified by the (−)nicotine administration. Both the changes of the NMDA-evoked [3 H]DA overflow in the NAc and PFC disappeared after 14 days withdrawal. The results show that chronic (−)nicotine differentially affects the NMDA-mediated [3 H]DA release in the PFC and NAc of the rat. 相似文献
12.
María P. Faillace María I. Keller Sarmiento Ruth E. Rosenstein 《Journal of neurochemistry》1996,67(2):623-628
Abstract: The effect of melatonin on [3 H]glutamate uptake and release in the golden hamster retina was studied. In retinas excised in the middle of the dark phase, i.e., at 2400 h, melatonin (0.1 and 10 n M ) significantly increased [3 H]glutamate uptake, and this effect persisted in a Ca2+ -free medium. On the other hand, melatonin significantly increased [3 H]glutamate release in retinas excised at 2400 h, but this effect was Ca2+ sensitive. Melatonin significantly increased 45 Ca2+ uptake by a crude synaptosomal fraction from retinas of hamsters killed at 2400 h. In retinas excised at 1200 h, melatonin had no effect on [3 H]glutamate uptake, [3 H]glutamate release, or 45 Ca2+ uptake at any concentration tested. Cyclic GMP analogues, i.e., 8-bromoguanosine 3',5'-cyclic monophosphate and 2'- O -dibutyrylguanosine 3',5'-cyclic monophosphate, significantly increased [3 H]glutamate uptake, [3 H]glutamate release, and 45 Ca2+ uptake by tissue removed at 1200 and 2400 h, suggesting that the effects of melatonin could correlate with a previously described effect of melatonin on cyclic GMP levels in the golden hamster retina. Taking into account the key role of glutamate in visual mechanisms, the results suggest the participation of melatonin in retinal physiology. 相似文献
13.
David A. Greenberg Edward C. Cooper Adrienne Gordon Ivan Diamond 《Journal of neurochemistry》1984,42(4):1062-1068
Abstract: Ethanol appears to enhance γ-aminobutyric acid (GABA)-mediated synaptic transmission. Using radioligand binding techniques, we investigated the possibility that the GABA-benzodiazepine receptor complex is the site responsible for this effect. Ethanol at concentrations up to 100 m M failed to alter binding of [3 H]flunitrazepam (FNZ), [3 H]Ro 15-1788, or [3 H]methyl-γ-carboline-3-carboxylate (MBCC) to benzodiazepine receptors, or of [3 H]muscimol to GABA receptors in rat brain membranes. Scatchard analyses of the binding of these radioligands at 4°C and 37°C revealed no significant effects of 100 m M ethanol on receptor affinity or number. A variety of drugs as well as chloride ion increased binding of [3 H]FNZ and/or [3 H]muscimol, but these influences were not modified by ethanol. These findings indicate that ethanol probably potentiates GABAergic neurotransmission at a signal transduction site beyond the GABA-benzodiazepine receptor complex. 相似文献
14.
Effect of Tetanus Toxin on Transmitter Release from the Substantia Nigra and Striatum In Vitro 总被引:4,自引:4,他引:0
G. L. Collingridge G. G. S. Collins J. Davies T. A. James M. J. Neal P. Tongroach 《Journal of neurochemistry》1980,34(3):540-547
Abstract: The effect of tetanus toxin on the uptake and release of radiolabelled transmitters from slices prepared from substantia nigra (SN) and striatum of rats has been investigated. Tetanus toxin-500–750 mouse lethal doses (MLD)-injected into the SN 6 h before preparing the slices significantly reduced the calcium-dependent, potassium-evoked release of [3 H]GABA. Endogenous GABA levels in the SN and [3 H]GABA uptake by nigral slices were unaffected by pretreatment with the toxin. Injections of tetanus toxin (1000–2000 MLD) into the striatum significantly reduced the calcium-dependent, potassium-evoked release of [14 C]GABA and also [3 H]dopamine, but had no effect on the K+ -evoked release of [3 H]5-hydroxytryptamine or [14 C]acetylcholine. It is concluded that tetanus toxin inhibits GABA release directly and not by interference with synthesis or inactivation processes. 相似文献
15.
Abstract: Addition of several polyamines, including spermidine and spermine, was effective in inhibiting binding of the antagonist ligand [3 H] 5, 7-dichlorokynurenic acid ([3 H]- DCKA) but not of the agonist ligand [3 H] glycine ([3 H] Gly) to a Gly recognition domain on the N -methyl-D-aspartic acid (NMDA) receptor ionophore complex in rat brain synaptic membranes. In contrast, [3 H] DCKA binding was significantly potentiated by addition of proposed polyamine antagonists, such as ifenprodil and (±)-α-(4-chlorophenyl)-4- [(4-fluorophenyl)methyl]-1-piperidine ethanol, with [3 H] Gly binding being unchanged. The inhibition by spermidine was significantly prevented by inclusion of ifenprodil. In addition, spermidine significantly attenuated the abilities of four different antagonists at the Gly domain to displace [3 H] DCKA binding virtually without affecting those of four different agonists. Phospholipases A2 and C and p -chloromercuribenzosulfonic acid were invariably effective in significantly inhibiting [3 H] DCKA binding with [3 H] Gly binding being unaltered. Moreover, the densities of [3 H] DCKA binding were not significantly different from those of [3 H]- Gly binding in the hippocampus and cerebral cortex, whereas the cerebellum had more than a fourfold higher density of [3 H] Gly binding than of [3 H] DCKA binding. These results suggest that the Gly domain may have at least two different forms based on the preference to agonists and antagonists in the rodent brain. 相似文献
16.
Abstract: Stores of methionine-enkephalin were labelled on the N -terminal by incubation of whole brain slices with [3 H]tyrosine (10 °Ci/ml). The 3 H radioactivity corresponding to the position of authentic Met-enkephalin after extraction on Amberlite XAD2 and separation by thin-layer chromatography was taken as an index of synthesis. Maximal incorporation of the labelled tyrosine into Met-enkephalin was attained after 4 h of incubation at 37°C and was inhibited in the presence of 10 μ M cycloheximide. Isolated nerve terminals failed to incorporate any [3 H]tyrosine. The labelled compound had opiatelike activity and consisted of the same five amino acids as an authentic standard. Incubations with leucine aminopeptidase indicated that the labelled tyrosine was on the N -terminus and removal of this tyrosine resulted in loss of opiate-like activity. The incorporation of [14 C]glycine, selected as an alternative precursor, was consistent with de novo synthesis and not N -terminal exchange. A radioimmunoassay was also used to quantify the amount of labelled Met-enkephalin. KCl (50 m M ) elicited a Ca2+ -dependent release of the synthesised [3 H]Met-enkephalin from whole brain slices and also from isolated nerve terminals. The release of Met-enkephalin radioimmunoactivity paralleled that of [3 H]met-enkephalin. Preliminary investigations have suggested that carbamyl choline inhibited this release and its effect was partially reversed by atropine. 相似文献
17.
DEMONSTRATION OF ACETYLCHOLINE RELEASE BY MEASURING EFFLUX OF LABELLED CHOLINE FROM CEREBRAL CORTICAL SLICES 总被引:4,自引:2,他引:2
Abstract— To demonstrate release of ACh in the absence of inhibition of cholinesterase, slices of cerebral cortex were incubated with [3 H]choline, after which they were placed in a tissue bath for superfusion. Hemicholinium (HC-3) increased the spontaneous efflux of [3 H]choline. Electrical stimulation at 4/s increased the efflux of [3 H]choline to the same extent whether the slices were stimulated early or late during superfusion. The effect of stimulation on efflux of [3 H]choline was abolished by tetrodotoxin and by the absence of calcium. The extent of choline efflux resulting from stimulation, as calculated from the specific radioactivity of the incubation medium, was the same when the slices were incubated with 0.1 or 1.0mM choline, but was less with lower concentrations of choline. We conclude that the increased efflux of [3 H]choline evoked by stimulation probably originates from stores of [3 H]ACh synthetized during incubation. 相似文献
18.
Abstract: The effects of AMPA and kainate on [3 H]dopamine release from fetal (embryonic day 15) rat mesencephalic neurons in primary culture were enhanced markedly in a dose-dependent fashion by cyclothiazide, a recently described inhibitor of AMPA receptor desensitization. The EC50 value for cyclothiazide was 2.2 ± 0.8 µ M . The release of [3 H]dopamine induced by both AMPA (or kainic acid) and the combination of AMPA (or kainic acid) with cyclothiazide was antagonized by specific antagonists like 6-cyano-7-nitroquinoxaline-2,3-dione or the noncompetitive benzodiazepine GYKI 52466. Unlike cyclothiazide, the lectin concanavalin A did not stimulate [3 H]dopamine release. These results established the involvement of AMPA-preferring receptors on [3 H]dopamine release from rat mesencephalic neurons in primary culture and provided further evidence for the existence of regulatory allosteric sites on AMPA receptor subunits. 相似文献
19.
Neurochemical Studies of the Mesolimbic Dopaminergic Pathway: Somatodendritic Mechanisms and GABAergic Neurones in the Rat Ventral Tegmentum 总被引:6,自引:4,他引:2
Abstract: The rat ventral tegmentum (containing dendrites and somata of mesolimbic neurones) contained 1.3 μg/g of dopamine, which was reduced to 40% of the control level by reserpine. Slices of ventral tegmentum were able to accumulate and release (elevated potassium or protoveratrine A) exogenous [3 H]dopamine. In parallel studies the uptake mechanism in ventral tegmentum was shown to be virtually identical to the nerve terminal uptake of [3 H]dopamine by slices of nucleus accumbens. The release of [3 H]dopamine was indistinguishable from that observed in substantia nigra, where there is substantial evidence for dendritic mechanisms. Basal adenylate cyclase activity was present, but dopamine-stimulated activity was not detected. A high GABA concentration (7.7 μmol/g) was present in ventral tegmentum, in conjunction with an uptake and a release mechanism for [3 H]GABA. GABA and muscimol elicited a small, reproducible efflux of [3 H]dopamine, but an interaction between dopamine and [3 H]GABA efflux was not observed. The results are in accord with transmitter roles for dopamine and GABA in the somatoden-dritic area of mesolimbic dopaminergic neurons. 相似文献
20.
Paulo F. Santos Maria S. Santos Arsélio P. Carvalho Carlos B. Duarte 《Journal of neurochemistry》1998,71(3):1086-1094
Abstract: We have investigated the effect of endogenous adenosine on the release of [3 H]acetylcholine ([3 H]ACh) in cultured chick amacrine-like neurons. The release of [3 H]ACh evoked by 50 m M KCl was mostly Ca2+ dependent, and it was increased in the presence of adenosine deaminase and in the presence of 1,3-dipropyl-8-cyclopentylxanthine (DPCPX), an adenosine A1 receptor antagonist. The effect of adenosine on [3 H]ACh release was sensitive to pertussis toxin (PTX) and was due to a selective inhibition of N-type Ca2+ channels. Ligand binding studies using [3 H]DPCPX confirmed the presence of adenosine A1 receptors in the preparation. Using specific inhibitors of the plasma membrane adenosine carriers and of the ectonucleotidases, we found that the extracellular accumulation of adenosine in response to KCl depolarization was due to the release of endogenous adenosine per se and to the extracellular conversion of released nucleotides into adenosine. Activation of adenosine A1 receptors was without effect on the intracellular levels of cyclic AMP under depolarizing conditions, but it inhibited the accumulation of inositol phosphates. Our results indicate that in cultured amacrine-like neurons, the Ca2+ -dependent release of [3 H]ACh evoked by KCl is under tonic inhibition by adenosine, which activates A1 receptors. The effect of adenosine on the [3 H]ACh release may be due to a direct inhibition of N-type Ca2+ channels and/or secondary to the inhibition of phospholipase C and involves the activation of PTX-sensitive G proteins. 相似文献