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1.
Nonreplication of heterochromatic chromosomes in a mealy bug,Planococcus citri (Coccoidea: Homoptera) 总被引:1,自引:1,他引:0
Uzi Nur 《Chromosoma》1966,19(4):439-448
In males of mealy bugs with the lecanoid chromosome system, the paternal set of chromosomes becomes heterochromatic in early embryogeny. In males of the mealy bug, Planococcus citri, the heterochromatic (H) set in testis sheath cells and in most of the oenocytes apparently did not replicate while the euchromatic (E) set was undergoing several cycles of endoreplication. In third instar males, testis sheath cells in endoanaphase and endotelophase exhibited 5H and either 40 or 80E chromosomes. The increase in the number of E chromosomes was attributed to the replication of only the E chromosomes. Oenocytes of third instar males had 0, 5, or 10H chromosomes and from 10 to 240E chromosomes. The oenocytes with 5H chromosomes had a mean of 50.8E chromosomes, and those with 10H chromosomes had a mean of 155.6E chromosomes. Nuclear and cell fusion was considered as a means of producing the various numbers of H and E chromosomes in oenocytes, and it was concluded that although nuclear fusion probably took place, the differences between the number of H and E chromosomes was at least in part due to replication of only the E chromosomes. The size of the H chromosomes was about the same in all the testis sheath cells and the oenocytes irrespective of the level of endopolyploidy for the E set. These H chromosomes apparently did not increase in polyteny, because they were only about half the size of the H chromosomes in prophase I of spermatogenesis. The significance of the nonreplication of the H set and the control of nonreplication are briefly discussed.This study was aided by a grant (GB-1585) from the National Science Foundation, Washington, D.C. 相似文献
2.
Dr. Walter A. Nelson-Rees 《Chromosoma》1963,14(1):1-17
Summary A reexamination of the second spermatogenic division of the mealy bug, Planococcus citri (Risso), a lecanoid coccid, has revealed hitherto unknown spindle activity of the euchromatic set of chromosomes during anaphase II. An initial large half spindle elaborated by the heterochromatic chromosomes in early metaphase, gives way to a less pronounced, but clearly visible bipolar spindle involving both sets of chromosomes at early anaphase. There is no lengthening of the spindle or cell, but the separation of the chromosomes occurs around the periphery of the cell with the aid of interzonal activity. The active participation of the euchromatic chromosome during the separation is furthermore inferred by the formation of bridges resulting from euchromatic-heterochromatic translocations. 相似文献
3.
Dr. H. Sharat Chandra 《Chromosoma》1963,14(3):310-329
Summary In the mealy bug, Planococcus citri, following high dosage paternal irradiation (60,000–120,000 rep), the survivors are mostly female (about 30–40% of the unirradiated control value) whereas very few males survive (about 5% of control value). After lower doses of paternal irradiation (P. I.), however, few or no females survive while the normal number of males (never less than the control value) survive.The females developing after high dosage P. I. are gynogenetic and are triploid or diploid or 3N/2N or 2N/N mosaics (Chandra 1963).The cytology of X1 embryos following 90,000 rep is described in this report, in comparison with data from embryos following lower doses (8,000 r) of P. I. and unirradiated controls, to illustrate the chromosomal mechanisms leading to the production of gynogenetic females and the probable reasons for lethality of X1 males after heavy P. I.It has been shown that triploid females stem from a fusion nucleus of the first and second polar bodies. This triploid polar nucleus, which normally participates in the formation of a polyploid sector in the young embryo, undertakes a successful embryogeny in many embryos when the zygote nucleus is unable to do so because of the heavily damaged paternal complement of chromosomes. Since the chromosomes are characterized by holokinetic activity, the irradiated paternal set manages to divide with the maternal complement but did not always segregate as successfully. Restitution divisions of the zygotic nuclei result in haploid, hyperhaploid, diploid and polyploid nuclei. Most of the diploid gynogenetic females probably originate from diploid nuclei of zygotic origin although it is possible that a few diploid females and the 2N/N mosaic females develop from polar bodies.From a dissertation submitted to the University of California, in partial satisfaction of the requirements for the degree of Doctor of Philosophy.Supported in part by a National Science Foundation Grant (No. G-9772) to Professor Spencer W. Brown.N. I. H. Predoctoral Trainee in Genetics 1961–1962. 相似文献
4.
Conventional types of cytogenetic studies with the mealybug, Planococcus citri (Risso), are possible with the use of genetic markers and meiotic analysis in the female. The loci of an eye-color mutant, salmon, and a wing-shape mutant, banjo, are linked with about 22 per cent recombination. These markers have been used in the identification and maintenance of lethals and rearrangements. All the cytologically identifiable rearrangements have proved to be reciprocal translocations, some symmetric, others, grossly asymmetric or otherwise complicated. No simple breakage products have been recovered. On the basis of their effects on crossing over, some of the lethals are believed to be associated with small rearrangements. The bivalents normally have one chiasma; only 1.2 per cent have two. Interference is decidedly decreased in chiasma formation in translocation heterozygotes, and in genetic recombination with suspected small rearrangements associated with lethals; it is also decreased, but less markedly, in genetic recombination with lethals in translocations. These various results are discussed in relationship to the holokinetic nature of the coccid chromosome, and natural increases in coccid chromosome number, as well as in regard to the effect of rearrangements on interference.Supported by grants from the National Science Foundation, currently GB 8196, and by a professorship (1968–69) for the senior author in the Miller Institute for Basic Research in Science.Dedicated to Dr. Sally Hughes-Schrader on the occasion of her seventy-fifth birthday. 相似文献
5.
Imré Foldi 《Cell and tissue research》1973,143(4):549-568
Résumé Le tube digestif des cochenilles se caractérise par la présence d'un dispositif intestinal appelé chambre filtrante. Celle-ci, formée par l'accolement des deux extrêmités de l'intestin moyen, est supposée intervenir dans le transit alimentaire en éliminant par «voie raccourcie» l'eau ingérée en excès, mais ce rôle est resté jusque là hypothétique.Chez Planococcus citri, l'étude cytologique et histochimique révèle des caractères propres aux parties de l'intestin moyen participant à la réalisation de ce dispositif. Ces caractères suggèrent une fonction particulière qui peut être liée à un mouvement de l'eau. Les cellules sont de taille très réduite. Par endroits, des différences ultrastructurales s'observent entre les deux anses accolées (présence de vacuoles et d'espaces extracellulaires développés). Les cellules non modifiées de l'anse de départ présentent un caractère morphologique particulier, le système d'espaces intercellulaires et un caractère histochimique dominant, l'importante quantité de glycogène. Les cellules non modifiées de l'anse de retour présentent une structure inhabituelle, les «expansions tubulaires» associées aux microvillosités. En absence du «plasmalemma mitochondrial complex» le passage de l'eau se ferait par osmose dans deux directions: d'une part à travers les épithéliums amincis, d'autre part par le réseau d'espaces intercellulaires.
Studies on the filter-chamber of Planococcus citri risso (Insecta, Homoptera)Histochemistry and ultrastructure
Summary The digestive tract of the coccids is characterized by the presence of a filter chamber formed by the apposition of the two extremities of the midgut. It is supposed that the filter chamber plays a role in the elimination of excess water by a short circuit but this assumption is still hypothetical.In Planococcus citri, cytological and histochemical studies have revealed structural peculiarities in those parts of the midgut which participate in the organization of the filter chamber, which suggest a particular function. The cells are highly reduced in size. In certain regions ultrastructural differences exist between the two apposed extremities of the midgut such as the presence of a vacuole and of highly developed extra-cellular spaces. These features may be related to water transport. The conventional cells of the proximal end of the midgut show the presence of a system of intercellular spaces and are very rich in glycogen. The conventional cells of the distal end of the midgut possess extracellular microtubules associated with the microvilli. In absence of a plasmalemma mitochondrial complex the transport of water could occur by osmosis across the thinned epithelium and the network of intercellular spaces.
Cet article recouvre en partie le travail d'une thèse de Docteur-Ingénieur soutenue à l'Université Paris VI en 1972. 相似文献
6.
Identification of Planococcus citri (Homoptera: Pseudococcidae) and the description of a new species
JENNIFER M. COX 《Systematic Entomology》1981,6(1):47-53
Planococcus pacificus sp.n. is described from the South Pacific, Australia and South East Asia. P.citricus is synonymized with P.citri. A key and illustrations are given to separate the species discussed from each other and from the similar P.ficus group. 相似文献
7.
Spermatogenesis is described in two eriococcid species and the observations are compared to those previously reported. In Gossyparia spuria the diploid chromosome number is 28 in both males and females. In the female all the chromosomes are euchromatic. In most male tissues 14 of the chromosomes are euchromatic (E) and 14 are heterochromatic (H). Prior to the first meiotic division in males the number of H chromosomes was reduced. During prophase I all the cells showed 14 E chromosomes and from 1 to over 9 H chromosomes. The range of chromosome numbers in metaphase I was similar to that in prophase I. All the chromosomes divided in anaphase I, and, following differential uncoiling at interkinesis, the E and H groups of chromosomes segregated from each other at anaphase II. Only the E groups formed sperm. The presence of a variable number of H chromosomes and a haploid number of E chromosomes in spermatogenesis suggested the presence of the multiple-D variant of the Comstockiella chromosome system. In this system some of the H chromosomes become euchromatic prior to prophase I of spermatogenesis and pair with their E homologues. All the remaining H chromosomes are thus univalents, while among the E elements, some are univalents and the rest are bivalents. The observed reduction in the number of H chromosomes in the first meiotic division which was previously attributed to pairing among the H chromosomes, is now interpreted to be the result of the return of some of the H chromosomes to a euchromatic state and to their subsequent pairing with their E homologues. Spermatogenesis in Eriococcus araucariae was similar to that of G. spuria except that the reduction in the number of H chromosomes was not as extensive. The chromosome systems of the two species are compared to those of other eriococcids and the differences are briefly discussed.Supported by grant GB1585 from the National Science Foundation, Washington, D. C. 相似文献
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9.
Molecular Evidence of Polyandry in the Citrus Mealybug,Planococcus citri (Hemiptera: Pseudococcidae)
Sofia G. Seabra Patricia G. Brás Vera Zina Elsa Borges da Silva Maria Teresa Rebelo Elisabete Figueiredo Zvi Mendel Octávio S. Paulo José Carlos Franco 《PloS one》2013,8(7)
The occurrence of polyandry in
Planococcus
citri
, presumed by earlier observations of mating behavior, was confirmed using microsatellite genotyping of pools of over 400 eggs resulting from controlled crosses of one female with two males. The genetic contribution of both mated males was confirmed in 13 out of 43 crosses. In three crosses it was possible to determine that only the first male fertilized the eggs, which may be due to sperm competition or unviable sperm supply. The microsatellite analysis also allowed the confirmation of aspects of the chromosomal inheritance detected previously in cytogenetic studies in
Planococcus
citri
, namely that only one of the alleles is transmitted by the male, indicating that the males are functionally haploid, supporting the observation of Paternal Genome Elimination (PGE) in these insects. 相似文献
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D.J. WILLIAMS 《Systematic Entomology》1974,42(2):217-220
Melanaspis samoana Lindinger the type-species of Lindingaspis is re-described and illustrated from syntype specimens and a lectotype is selected. 相似文献
13.
YAIR BEN-DOV 《Systematic Entomology》1988,13(4):387-392
Abstract. The mealybug Trabutina mannipara (Hemprich & Ehrenberg, 1829) is described and illustrated, and a neotype female selected from topotypic material collected in the Sinai Peninsula. A lectotype female is designated for Trabutina palestina Bodenheimer (1927) which is shown to be a junior synonym of T.mannipara. 相似文献
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15.
R.S. Sreerag S. Nishanth Kumar 《Archives Of Phytopathology And Plant Protection》2016,49(7-8):149-156
Rhizoecus amorphophalli is a polyphagous and very serious sucking insect pest on the root and tuber of plants. White powdery mealy substance associated with R. amorphophalli, gives protection to the insect. Mealy substance was isolated using a stereo zoom binocular microscope. Purity of the mealy substance was analysed using High-Performance Liquid Chromatography and Thin-layer chromatography. The structure of mealy substance was determined based on extensive spectroscopic analyses (FTIR, 1H NMR, 13C NMR). Based on the NMR data, the mealy substance was identified as a long-chained aliphatic hydrocarbon (ester) containing 30 carbon atoms. 相似文献
16.
Dr. Uzi Nur 《Chromosoma》1963,14(2):123-139
Summary Meiotic parthenogenesis of a type not previously described was found in Pulvinaria hydrangeae
Steinweden. During diakinesis 8 bivalents were formed. At prometaphase the spindle was tripolar but anaphase I was bipolar and normal. After completion of division of the primary oocyte, the following sequence occurred: 1. polar body I divided, usually into 3 products; 2. the secondary oocyte divided to yield the egg pronucleus and polar body II; 3. the egg pronucleus divided into its two haploid products; and 4. the second polar body divided. The products of the egg pronucleus fused while dividing to restore the diploid chromosome number; this division may be equated to the first cleavage division. The products of the polar bodies did not take part in the formation of the embryo proper or the mycetocytes.Among the embryos produced by females of two out of the three populations studied some of the embryos showed a heterochromatic chromosome set, characteristic of males in this and related families. The reproductive system of the females as well as the eggs did not contain any sperm; thus the male embryos were apparently produced parthenogenetically.The euchromatic and heterochromatic chromosome sets were genetically identical, since they both originated from the egg pronucleus by mitosis. The heterochromatization of one set but not the other might be due in part to a previous difference in their position in the cytoplasm.The females were completely homozygous yet they produced male and female embryos. Thus it appears that sex determination in the group does not depend on the segregation of genetic factors in either males or females.In addition to male and female embryos, three types of degenerating embryos were observed. It is believed that these embryos were formed by polyploid somatic cells which invaded abnormal eggs and embryos and took over development. 相似文献
17.
Uzi Nur 《Chromosoma》1969,28(3):280-297
The B-chromosomes (B's; supernumeraries) of the mealy bug, Pseudococcus obscurus Essig, segregate preferentially into the two functional products of male meiosis. This segregation thus serves as an accumulation mechanism. A cytological study of a population from Oakland, California, confirmed the results obtained earlier that the B's are harmful and are maintained only because of their accumulation mechanism. The wild females were studied directly. The number of B's in the males was determined by analyzing ten or more daughters of females without B's (0B females) after these were inseminated by wild males. The 0B females were exposed to the wild males in screen cages. The analysis of 4732 daughters of 231 caged females indicated that among the males which inseminated these females, there were 19.9% 0B males and the mean number of B's was 1.46 ± 0.07. Among 224 wild females which were collected at about the same time there were 12.5% 0B females, and the mean number of B's was 1.88 ± 0.09. Since the frequencies of the B's in the population changed only slightly from generation to generation, the expected zygotes of this generation were assumed to be similar to those from which both the males and the females developed. The expected zygotes were calculated from the observed frequencies of the B's among the sperm and the known rates of transmission in females. The zygotes were very similar to the females but quite different from the males. It was concluded, therefore, that the B's had little or no effect on the females carrying them, but reduced the fitness of the males. The fitness of the 0B, 1B, 2B, 3B and 4B males was calculated to be 1.00, 0.64, 0.56, 0.38 and 0.20 respectively. The rate of transmission of the B's decreased with the increase in the number of B's, from 0.84 in 1B males to 0.51 in 4B males. This decrease, and the decrease in male fitness with the increase in the number of B's are expected to help stabilize the number of B's in the population.This paper is dedicated to Professor Sally Hughes-Schrader on the occasion of her seventy-fifth birthday.Supported by grants GB 1585 and GB 6745 from the National Science Foundation, Washington, D. C. 相似文献
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Anegunda Shankar Dinesh 《Biocontrol Science and Technology》2011,21(5):523-533
The citrus mealybug, Planococcus citri (Risso) (Hemiptera: Pseudococcidae) is a serious pest of economically important crops worldwide. The apefly, Spalgis epius (Westwood) (Lepidoptera: Lycaenidae) is a potential predator of various species of mealybugs. Earlier investigation on its daily preying capacity and preference for prey stages on P. citri is incomplete. Hence, a study was conducted to find out the daily prey consumption ability and preference for prey stages by different larval instars of S. epius reared on P. citri in the laboratory. Through the 8-day developmental period with four larval instars, the daily prey consumption of S. epius increased from the first to the seventh day and decreased on the eighth day prior to the prepupal stage. Generally, there was a significant difference in the prey consumption on different days. When the prey stages were offered separately, the first to fourth instar larva of S. epius consumed, respectively, a mean of 199.6, 722.6, 1908.8, and 4625.6 eggs or 21.5, 77.0, 168.5, and 670.5 nymphs or 3.2, 7.2, 16.0, and 35.1 adults of P. citri. When an S. epius larva was fed on P. citri eggs, nymphs and adults separately, it consumed a mean of 7456.7 eggs, 937.6 nymphs, or 62.3 adults during its entire development. When the prey stages were offered all together, a single S. epius larva consumed 2618.4 eggs, 170.4 nymphs, and 39.7 adults of P. citri throughout its entire development. The study revealed that S. epius is a voracious predator of P. citri and thus could be utilized as a major biological control agent. 相似文献
20.
An experimental study of morphological variation in mealybugs (Homoptera: Coccoidea: Pseudococcidae)
JENNIFER M. COX 《Systematic Entomology》1983,8(4):361-382
Abstract Cultures of a number of species of mealybug were reared under a variety of controlled environments and the effects of factors such as temperature, relative humidity and host plant on the morphology of the adult females examined. It was found that temperature had the greatest effect. The lengths of the appendages and setae, and the numbers of wax producing pores and ducts, vary over a wide range within a single species when specimens are reared at different temperatures. The relationship with temperature is generally linear, with size and pore numbers increasing with decreased temperature. However, some characters, noticeably the numbers of tubular ducts, reach their highest values at an intermediate temperature. It is suggested that while variation in size is a direct response to temperature, other characters are influenced by size or by a combination of size and environmental factors acting independently. Rearing mealybugs under different environmental conditions in order to induce morphological variation is shown to be a useful technique for resolving difficult species complexes. 相似文献