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1.
Ravindra Patil Manoj Oak Anuja Deshpande Shubhada Tamhankar 《Molecular breeding : new strategies in plant improvement》2018,38(11):136
Phytoene synthase-1 (Psy-1) homoeologs are associated with yellow pigment content (YPC) in endosperm of durum and bread wheat. In the present study, microsatellite variation in promoter region of Psy-A1 was identified in durum wheat and marker Psy-1SSR, targeting the microsatellite variation was developed which amplifies variation in Psy-A1 and Psy-B1 loci simultaneously. Psy-A1SSR was mapped within QYp.macs-7A, a major QTL for YPC identified earlier in PDW 233/Bhalegaon 4 population. Marker Psy-A1SSR was further validated in two different RIL populations and a set of 222 tetraploid wheat accessions including less cultivated tetraploid wheat species. Eight alleles of Psy-A1SSR were identified in 222 wheat accessions, while seven alleles were observed for Psy-B1SSR. Variation at Psy-A1SSR showed significant association with YPC, whereas no association was observed with Psy-B1SSR. Marker-assisted introgression of Psy-A1SSRe allele from PDW 233, to durum wheat cultivars MACS 3125 and HI 8498 resulted in improvement of YPC. Backcrossed BC3F2:4 and BC2F2:3 lines selected using Psy-A1SSR showed 89 to 98% gain in YPC over recurrent parents indicating robustness of marker. The marker can thus be utilized in marker-assisted improvement of YPC in durum wheat cultivars. 相似文献
2.
Sang Heon Kim Jae Yoon Kim Dae Yeon Kim Jin Seok Yoon Woo Joo Jung Inés Yacoubi Yong Weon Seo 《Genes & genomics.》2016,38(10):939-948
Durum wheat (Triticum turgidum ssp. durum) is one of the main species of cultivated wheat. In arid and semi-arid areas, salinity stress reduces durum wheat productivity. This study used 26 durum wheat accessions from semi-arid regions in Tunisia to analyze plant tolerance to salt stress. Salt stress was experimentally applied by regularly submerging pots in NaCl solution. The salt tolerance trait index (STTI) and salt tolerance index (STI) of various growth parameters were used as criteria to select for salt tolerance. Analysis of genetic relationships was carried out to determine the genetic distance between durum wheat accessions. Based on simple sequence repeats analysis, a molecular marker for salt stress resistance in durum wheat was developed. Salt-treated plants had reduced morphological traits compared to control plants. Most STTIs in all genotypes were below 100 %. Based on STI, 8 accessions were found to be salt-resistant, 16 were salt-moderate, two were salt-susceptible. Analysis of the genetic relationships among 28 Tunisian durum wheat accessions revealed that landraces of the same nominal type are closely related. Of the 94 SSR primers investigated, three were selected and used to design sequence characterized amplified region (SCAR) primers. One SCAR primer pair, KUCMB_Xgwm403_2, produced a 207 bp band that was present in salt-resistant durum wheat lines but absent in salt-susceptible lines. The results suggest that KUCMB_Xgwm403_2 could be a potential genetic tag for salt-tolerant durum wheats. 相似文献
3.
Murat Akkurt Leocir Welter Erika Maul Reinhard Töpfer Eva Zyprian 《Molecular breeding : new strategies in plant improvement》2007,19(2):103-111
Sequence-characterized amplified regions markers (SCARs) were developed from six randomly amplified polymorphic DNA (RAPD)
markers linked to the major QTL region for powdery mildew (Uncinula necator) resistance in a test population derived from the cross of grapevine cultivars “Regent” (resistant) × “Lemberger”(susceptible).
RAPD products were cloned and sequenced. Primer pairs with at least 21 nucleotides primer length were designed. All pairs
were tested in the F1 progeny of “Regent” × “Lemberger”. The SCAR primers resulted in the amplification of specific bands
of expected sizes and were tested in additional genetic resources of resistant and susceptible germplasm. All SCAR primer
pairs resulted in the amplification of specific fragments. Two of the SCAR markers named ScORA7-760 and ScORN3-R produced
amplification products predominantly in resistant individuals and were found to correlate to disease resistance. ScORA7-760,
in particular, is suitable for marker-assisted selection for powdery mildew resistance and to facilitate pyramiding powdery
mildew resistance genes from various sources. 相似文献
4.
F. Chen H.-X. Xu F.-Y. Zhang X.-C. Xia Z.-H. He D.-W. Wang Z.-D. Dong K.-H. Zhan X.-Y. Cheng D.-Q. Cui 《Molecular breeding : new strategies in plant improvement》2011,28(2):153-161
The puroindoline genes (Pina and Pinb) are the functional components of the common or bread wheat (Triticum aestivum L.) grain hardness locus that are responsible for kernel texture. In this study, four puroindoline b-2 variants were physically mapped using nulli-tetrosomic lines of bread wheat cultivar Chinese Spring and substitution lines
of durum wheat (Triticum turgidum L.) cultivar Langdon. Results indicated that Pinb-2v1 was on 7D of Chinese Spring, Pinb-2v2 on 7B of Chinese Spring, Pinb-2v3 on 7B of Chinese Spring and Langdon, and Pinb-2v4 on 7A of Chinese Spring and Langdon. A new puroindoline b-2 variant, designated Pinb-2v5, was identified at the puroindoline b-2 locus of durum wheat cultivar Langdon, with a difference of only five single nucelotide polymorphisms compared with Pinb-2v4. Sequencing results indicated that, in comparison with the Pinb-2v3 sequence (AM99733 and GQ496618 with one base-pair modification of G to T at 6th position, designated Pinb-2v3a) in bread wheat cultivar Witchta, the coding region of Pinb-2v3 in 12 durum wheat cultivars had a single nucleotide change from T to C at the 311th position, resulting in a corresponding
amino acid change from valine to alanine at the 104th position. This new allele was designated Pinb-2v3b. The study of puroindoline b-2 gene polymorphism in CIMMYT and Italian durum wheat germplasm and discovery of a novel puroindoline b-2 variant could provide useful information for further understanding the molecular and genetic basis of kernel hardness and
illustrating gene duplication events in wheat. 相似文献
5.
S. Fondevilla Z. Satovic D. Rubiales M. T. Moreno A. M. Torres 《Molecular breeding : new strategies in plant improvement》2008,21(4):439-454
Aschochyta blight, caused by Mycosphaerella pinodes, is one of the most economically serious pea pathogens, particularly in winter sowings. The wild Pisum sativum subsp. syriacum accession P665 shows good levels of resistance to this pathogen. Knowledge of the genetic factors controlling resistance
to M. pinodes in this wild accession would facilitate gene transfer to pea cultivars; however, previous studies mapping resistance to M. pinodes in pea have never included this wild species. The objective of this study was to identify quantitative trait loci (QTL) controlling
resistance to M. pinodes in P. sativum subsp. syriacum and to compare these with QTLs previously described for the same trait in P. sativum. A population formed by 111 F6:7 recombinant inbred lines derived from a cross between accession P665 and a susceptible pea cultivar (Messire) was analysed
using morphological, isozyme, RAPD, STS and EST markers. The map developed covered 1214 cM and contained 246 markers distributed
in nine linkage groups, of which seven could be assigned to pea chromosomes. Six QTLs associated with resistance to M. pinodes were detected in linkage groups II, III, IV and V, which collectively explained between 31 and 75% of the phenotypic variation
depending of the trait. While QTLs MpIII.1 and MpIII.2 were detected both for seedlings and field resistance, MpV.1 and MpII.1 were specific for growth chamber conditions and MpIII.3 and MpIV.1 for field resistance. Quantitative trait loci MpIII.1, MpII.1, MpIII.2 and MpIII.3 may coincide with other QTLs associated with resistance to M. pinodes previously described in P.
sativum. Four QTLs associated with earliness of flowering were also identified. While dfIII.2 and dfVI.1, may correspond with other genes and QTLs controlling earliness in P. sativum, dfIII.1 and dfII.1 may be specific to P. sativum subsp. syriacum. Flowering date and growth habit were strongly associated with resistance to M. pinodes in the field evaluations. The relation observed between earliness, growth habit and resistance to M. pinodes is discussed. 相似文献
6.
He XY Zhang YL He ZH Wu YP Xiao YG Ma CX Xia XC 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(2):213-221
Phytoene synthase (Psy), a critical enzyme in the carotenoid biosynthetic pathway, demonstrated high association with the
yellow pigment (YP) content in wheat grain. Characterization of Psy genes and the development of functional markers for them
are of importance for marker-assisted selection in wheat breeding. In this study, the full-length genomic DNA sequence of
a Psy gene (Psy-A1) located on chromosome 7A, was characterized by in silico cloning and experimental validation. The cloned Psy-A1 comprises six exons and five introns, 4,175 bp in total, and an ORF of 1,284 bp. A co-dominant marker, YP7A, was developed based on polymorphisms of two haplotypes of Psy-A1, yielding 194 and 231-bp fragments in cultivars with high and low YP content, respectively. The marker YP7A was mapped on chromosome 7AL using an RIL population from cross PH82-2/Neixing 188, and a set of Chinese Spring nullisomic–tetrasomic
lines and ditelosomic line 7AS. Psy-A1, co-segregating with the STS marker YP7A, was linked to SSR marker Xwmc809 on chromosome 7AL with a genetic distance of 5.8 cM, and explained 20–28% of the phenotypic variance for YP content across
three environments. A total of 217 Chinese wheat cultivars and advanced lines were used to validate the association between
the polymorphic band pattern and grain YP content. The results showed that the functional marker YP7A was closely related to grain YP content and, therefore, could be used in wheat breeding programs targeting of YP content
for various wheat-based products.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
7.
Bolibok H Gruszczyńska A Hromada-Judycka A Rakoczy-Trojanowska M 《Cellular & molecular biology letters》2007,12(4):523-535
This study was conducted in order to identify quantitative trait loci (QTLs) for the in vitro culture response of winter rye (Secale cereale L.) immature embryos and immature inflorescences. A genetic linkage map comprising 67 SSRs, 9 ISSRs, 13 SAMPLs, 7 RAPDs,
2 SCARs and one EST marker was created based on the analyses of 102 recombinant inbred lines from the cross between lines
L318 (which has a good response in tissue cultures) and L9 (which is unable to regenerate plants from somatic tissues and
anthers). The map spans 979.2 cM, and the average distance between markers is 9.9 cM. Two characteristics were evaluated:
callus induction (CI) and somatic embryogenesis ability (SE). They were expressed as the percentage of immature embryos/inflorescences
producing callus (designated ECI/ICI) and the percentage of explants producing somatic embryos (ESE/ISE). All the analysed
traits showed continuous variation in the mapping population but a non-normal frequency distribution. We identified nine putative
QTLs controlling the tissue culture response of rye, explaining up to 41.6% of the total phenotypic variation: two QTLs for
ECI — eci-1, eci-2; 4 for ESE — ece-1, ese-2, ese-3, ese-4; 2 for ICI — ici-1, ici2; and 1 for ISE — ise-1. They were detected on chromosomes 1R, 4R, 5R, 6R and 7R. 相似文献
8.
Ayala-Navarrete L Bariana HS Singh RP Gibson JM Mechanicos AA Larkin PJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,116(1):63-75
Rusts and barley yellow dwarf virus (BYDV) are among the main diseases affecting wheat production world wide for which wild
relatives have been the source of a number of translocations carrying resistance genes. Nevertheless, along with desirable
traits, alien translocations often carry deleterious genes. We have generated recombinants in a bread wheat background between
two alien translocations: TC5, ex-Thinopyrum (Th) intermedium, carrying BYDV resistance gene Bdv2; and T4m, ex-Th. ponticum, carrying rust resistance genes Lr19 and Sr25. Because both these translocations are on the wheat chromosome arm 7DL, homoeologous recombination was attempted in the double
hemizygote (TC5/T4m) in a background homozygous for the ph1b mutation. The identification of recombinants was facilitated by the use of newly developed molecular markers for each of the
alien genomes represented in the two translocations and by studying derived F2, F3 and doubled haploid populations. The occurrence of recombination was confirmed with molecular markers and bioassays on families
of testcrosses between putative recombinants and bread wheat, and in F2 populations derived from the testcrosses. As a consequence it has been possible to derive a genetic map of markers and resistance
genes on these previously fixed alien linkage blocks. We have obtained fertile progeny carrying new tri-genomic recombinant
chromosomes. Furthermore we have demonstrated that some of the recombinants carried resistance genes Lr19 and Bdv2 yet lacked the self-elimination trait associated with shortened T4 segments. We have also shown that the recombinant translocations
are fixed and stable once removed from the influence of the ph1b. The molecular markers developed in this study will facilitate selection of individuals carrying recombinant Th. intermedium–Th. ponticum translocations (Pontin series) in breeding programs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
9.
S. Achtar M. Y. Moualla A. Kalhout M. S. Röder N. MirAli 《Russian Journal of Genetics》2010,46(11):1320-1326
Genetic diversity among 49 wheat varieties (37 durum and 12 bread wheat) was assayed using 32 microsatellites representing
34 loci covering almost the whole wheat genome. The polymorphic information content (PIC) across the tested loci ranged from
0 to 0.88 with average values of 0.57 and 0.65 for durum and bread wheat respectively. B-genome had the highest mean number
of alleles (10.91) followed by A genome (8.3) whereas D genome had the lowest number (4.73). The correlation between PIC and
allele number was significant in all genome groups accounting for 0.87, 074 and 0.84 for A, B and D genomes respectively,
and over all genomes, the correlation was higher in tetraploid (0.8) than in hexaploid wheat varieties (0.5). The cluster
analysis discriminated all varieties and clearly divided the two ploidy levels into two separate clusters that reflect the
differences in genetic diversity within each cluster. This study demonstrates that microsatellites markers have unique advantages
compared to other molecular and biochemical fingerprinting techniques in revealing the genetic diversity in Syrian wheat varieties
that is crucial for wheat improvement. 相似文献
10.
Supriya Kumari Vandana Jaiswal Vinod Kumar Mishra Rajneesh Paliwal Harindra Singh Balyan Pushpendra Kumar Gupta 《Physiology and Molecular Biology of Plants》2018,24(5):909-920
Grain traits are important agronomic attributes with the market value as well as milling yield of bread wheat. In the present study, quantitative trait loci (QTL) regulating grain traits in wheat were identified. Data for grain area size (GAS), grain width (GWid), factor form density (FFD), grain length-width ratio (GLWR), thousand grain weight (TGW), grain perimeter length (GPL) and grain length (GL) were recorded on a recombinant inbred line derived from the cross of NW1014?×?HUW468 at Meerut and Varanasi locations. A linkage map of 55 simple sequence repeat markers for 8 wheat chromosomes was used for QTL analysis by Composite interval mapping. Eighteen QTLs distributed on 8 chromosomes were identified for seven grain traits. Of these, five QTLs for GLWR were found on chromosomes 1A, 6A, 2B, and 7B, three QTLs for GPL were located on chromosomes 4A, 5A and 7B and three QTLs for GAS were mapped on 5D and 7D. Two QTLs were identified on chromosomes 4A and 5A for GL and two QTLs for GWid were identified on chromosomes 7D and 6A. Similarly, two QTLs for FFD were found on chromosomes 1A and 5D. A solitary QTL for TGW was identified on chromosome 2B. For several traits, QTLs were also co-localized on chromosomes 2B, 4A, 5A, 6A, 5D, 7B and 7D. The QTLs detected in the present study may be validated for specific crosses and then used for marker-assisted selection to improve grain quality in bread wheat. 相似文献
11.
The genus of Secale has many agronomically important characters. In order to use the best of this species, markers tracking the rye chromatin
incorporated into wheat must be developed. In this study, one rye genome-specific random amplified polymorphic DNA (RAPD)
marker was isolated from Secale africanum (Ra genome). Two cloned markers, named OPP131165 and OPP13662, were 1165 bp and 662 bp, respectively. Sequence analysis revealed that OPP131165 was highly homologous to a part of a new class of transposon-like gene called the Revolver family, and OPP13662 was partially similar to LTR gypsy-like retrotransposon. Fluorescence in situ hybridization (FISH) showed only OPP131165 localized within the whole arms of rye except their terminal regions and no signal was detected on wheat chromosomes, while
OPP13662 had no hybridization signal detected on wheat and rye genomes. Based on these sequences, two pairs of sequence-characterized
amplified region (SCAR) primers were designed, and the resulted SCAR markers were able to target both cultivated and wild
Secale species. The FISH patterns and the two SCAR markers should be able to identify and track all wheat-rye translocation lines,
especially the S. africanum chromatin. 相似文献
12.
Genqiao Li Tilin Fang Hongtao Zhang Chaojie Xie Hongjie Li Tsomin Yang Eviatar Nevo Tzion Fahima Qixin Sun Zhiyong Liu 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,119(3):531-539
Powdery mildew caused by Blumeria graminis f. sp. tritici is an important wheat disease in China and other parts of the world. Wild emmer (Triticum turgidum var. dicoccoides) is the immediate progenitor of cultivated tetraploid and hexaploid wheats and thus an important resource for wheat improvement.
Wild emmer accession IW2 collected from Mount Hermon, Israel, is highly resistant to powdery mildew at the seedling and adult
plant stages. Genetic analysis using an F2 segregating population and F2:3 families, derived from a cross between susceptible durum cultivar Langdon and wild emmer accession IW2, indicated that a
single dominant gene was responsible for the resistance of IW2. Bulked segregant and molecular marker analyses detected that
six polymorphic SSR, one ISBP, and three EST-STS markers on chromosome 3BL bin 0.63–1.00 were linked to the resistance gene.
Allelic variations of resistance-linked EST-STS marker BE489472 revealed that the allele was present only in wild emmer but absent in common wheat. Segregation distortion was observed for
the powdery mildew resistance allele and its linked SSR markers with preferential transmission of Langdon alleles over IW2
alleles. The resistance gene was introgressed into common wheat by backcrossing and marker-assisted selection. Since no designated
powdery mildew resistance gene has been found on chromosome 3BL, the resistance gene derived from wild emmer accession IW2
appears to be new one and was consequently designated Pm41.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
13.
Azhaguvel P Rudd JC Ma Y Luo MC Weng Y 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(3):555-564
The greenbug, Schizaphis graminum (Rondani), is an important aphid pest of small grain crops especially wheat (Triticum aestivum L., 2n = 6x = 42, genomes AABBDD) in many parts of the world. The greenbug-resistance gene Gb3 originated from Aegilops tauschii Coss. (2n = 2x = 14, genome DtDt) has shown consistent and durable resistance against prevailing greenbug biotypes in wheat fields. We previously mapped Gb3 in a recombination-rich, telomeric bin of wheat chromosome arm 7DL. In this study, high-resolution genetic mapping was carried
out using an F2:3 segregating population derived from two Ae. tauschii accessions, the resistant PI 268210 (original donor of Gb3 in the hexaploid wheat germplasm line ‘Largo’) and susceptible AL8/78. Molecular markers were developed by exploring bin-mapped
wheat RFLPs, SSRs, ESTs and the Ae. tauschii physical map (BAC contigs). Wheat EST and Ae. tauschii BAC end sequences located in the deletion bin 7DL3-0.82–1.00 were used to design STS (sequence tagged site) or CAPS (Cleaved
Amplified Polymorphic Sequence) markers. Forty-five PCR-based markers were developed and mapped to the chromosomal region
spanning the Gb3 locus. The greenbug-resistance gene Gb3 now was delimited in an interval of 1.1 cM by two molecular markers (HI067J6-R and HI009B3-R). This localized high-resolution
genetic map with markers closely linked to Gb3 lays a solid foundation for map based cloning of Gb3 and marker-assisted selection of this gene in wheat breeding. 相似文献
14.
Federico Luis Iniguez-Luy Lewis Lukens Mark W. Farnham Richard M. Amasino Thomas C. Osborn 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2009,120(1):31-43
Publicly available genomic tools help researchers integrate information and make new discoveries. In this paper, we describe
the development of immortal mapping populations of rapid cycling, self-compatible lines, molecular markers, and linkage maps
for Brassica rapa and B. oleracea and make the data and germplasm available to the Brassica research community. The B. rapa population consists of 160 recombinant inbred (RI) lines derived from the cross of highly inbred lines of rapid cycling and
yellow sarson B. rapa. The B. oleracea population consists of 155 double haploid (DH) lines derived from an F1 cross between two DH lines, rapid cycling and broccoli.
A total of 120 RFLP probes, 146 SSR markers, and one phenotypic trait (flower color) were used to construct genetic linkage
maps for both species. The B. rapa map consists of 224 molecular markers distributed along 10 linkage groups (A1–A10) with a total distance of 1125.3 cM and
a marker density of 5.7 cM/marker. The B. oleracea genetic map consists of 279 molecular markers and one phenotypic marker distributed along nine linkage groups (C1–C9) with
a total distance of 891.4 cM and a marker density of 3.2 cM/marker. A syntenic analysis with Arabidopsis thaliana identified collinear genomic blocks that are in agreement with previous studies, reinforcing the idea of conserved chromosomal
regions across the Brassicaceae. 相似文献
15.
M. N. Melnikova S. D. Pavlov A. A. Kolesnikov N. B. Petrov 《Russian Journal of Genetics》2010,46(6):699-704
Using AP-PCR, the genome of Kamchatka mykiss (Parasalmo (O.) mykiss) was examined. Polymorphic fragments, implying geographic differences among the samples, were selected, cloned, and sequenced.
Based on these sequences, longer, specific SCAR primers were selected and constructed. Using the BLAST software program, the
sequences were analyzed for analogy to those from the GenBank database. It seemed likely that all sequences obtained belonged
to earlier unexamined repeated sequences, variable in the populations of the species of interest. A total of seven SCAR markers,
characterized by population-significant variability of the DNA products in Kamchatka geographic group of rainbow trout were
constructed. These markers can be used for further investigation of the species Parasalmo (O.) mykiss. The SCAR marker sequences were deposited in GenBank under the accession numbers EU805500 to EU805506. 相似文献
16.
Gurung S Mamidi S Bonman JM Jackson EW del Río LE Acevedo M Mergoum M Adhikari TB 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(6):1029-1041
Tan spot, caused by Pyrenophora tritici-repentis, is a major foliar disease of wheat worldwide. Host plant resistance is the best strategy to manage this disease. Traditionally,
bi-parental mapping populations have been used to identify and map quantitative trait loci (QTL) affecting tan spot resistance
in wheat. The association mapping (AM) could be an alternative approach to identify QTL based on linkage disequilibrium (LD)
within a diverse germplasm set. In this study, we assessed resistance to P. tritici-repentis races 1 and 5 in 567 spring wheat landraces from the USDA-ARS National Small Grains Collection (NSGC). Using 832 diversity
array technology (DArT) markers, QTL for resistance to P. tritici-repentis races 1 and 5 were identified. A linear model with principal components suggests that at least seven and three DArT markers
were significantly associated with resistance to P. tritici-repentis races 1 and 5, respectively. The DArT markers associated with resistance to race 1 were detected on chromosomes 1D, 2A, 2B,
2D, 4A, 5B, and 7D and explained 1.3–3.1% of the phenotypic variance, while markers associated with resistance to race 5 were
distributed on 2D, 6A and 7D, and explained 2.2–5.9% of the phenotypic variance. Some of the genomic regions identified in
this study correspond to previously identified loci responsible for resistance to P. tritici-repentis, offering validation for our AM approach. Other regions identified were novel and could possess genes useful for resistance
breeding. Some DArT markers associated with resistance to race 1 also were localized in the same regions of wheat chromosomes
where QTL for resistance to yellow rust, leaf rust and powdery mildew, have been mapped previously. This study demonstrates
that AM can be a useful approach to identify and map novel genomic regions involved in resistance to P. tritici-repentis. 相似文献
17.
M. Muñoz-Amatriaín A. M. Castillo X. W. Chen L. Cistué M. P. Vallés 《Molecular breeding : new strategies in plant improvement》2008,22(1):119-129
In cereals, albinism is a major obstacle to produce doubled haploids (DH) for breeding programs. In order to identify QTLs
for green plant percentage in barley anther culture, a specific population was developed. This population, consisting of 100
DH lines, was generated by crossing the model cultivar for anther culture “Igri” with an albino-producing DH line (DH46) selected
from Igri × Dobla, in search of a maximum segregation for the trait and minimum for the other anther culture variables. A
combination of bulked segregant analysis and AFLP methodology was used to identify markers linked to the trait. A linkage
map was constructed using these AFLPs, together with RAPD, STS and SSR markers. This study identified a new QTL for green
plant percentage on chromosome 3H and confirmed the previously reported one on chromosome 5H. Up to 65.2% of the phenotypic
variance for this trait was explained by the additive effects of these two QTLs. Thirty elite cultivars of barley from different
origin, row type, growth habit and end use, were selected to validate these QTLs. Since two of the markers linked to the QTLs
were AFLPs, we successfully converted them into simple PCR-based SCAR markers. Only the SSR HVM60, on chromosome 3H, was significantly
associated with the trait, explaining near 20% of the phenotypic variance. Among the allelic variants identified for this
marker, HVM60-120bp was associated with the highest values of green plant percentage. 相似文献
18.
Genetic transformation of wheat, using biolistics or Agrobacterium, underpins a range of specific research methods for identifying genes and studying their function in planta. Transgenic approaches to study and modify traits in durum wheat have lagged behind those for bread wheat. Here we report
the use of Agrobacterium strain AGL1, with additional vir genes housed in a helper plasmid, to transform and regenerate the durum wheat variety Ofanto. The use of the basic pSoup
helper plasmid with no additional vir genes failed to generate transformants, whereas the presence of either virG542 or the 15 kb Komari fragment containing virB, virC and virG542 produced transformation efficiencies of between 0.6 and 9.7%. Of the 42 transgenic plants made, all but one (which set very
few seeds) appeared morphologically normal and produced between 100 and 300 viable seeds. The transgene copy number and the
segregation ratios were found to be very similar to those previously reported for bread wheat. We believe that this is the
first report describing successful genetic transformation of tetraploid durum wheat (Triticum turgidum L. var. durum) mediated by Agrobacterium tumefaciens using immature embryos as the explant. 相似文献
19.
In a previous study, several quantitative trait loci (QTLs) affecting vegetative propagation traits were detected in a hybrid
cross between Eucalyptus tereticornis and Eucalyptus globulus. The objective of this work was to confirm stable QTL linked markers (detected in different years) for propagation traits
in an independent set of the same segregating population and in two related crosses involving the original E. globulus parent. Phenotypic averages of groups of individuals carrying alternative allelic forms of the stable QTL linked markers
were statistically tested for significant differences. Adventitious rooting and petrification marker–trait associations, detected
previously in the E. tereticornis parent, were verified in an independent sample of the original progeny. In the E. globulus parent, the QTL linked marker was only verified in one related genetic background. Verification was possible only for high-effect
QTL linked markers. This study highlights the importance of sample size in QTL detection for low-heritability traits. 相似文献
20.
Kaiye Liu Hao Xu Gang Liu Panfeng Guan Xueyao Zhou Huiru Peng Yingyin Yao Zhongfu Ni Qixin Sun Jinkun Du 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2018,131(4):839-849