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1.
A novel bacterial strain, designated T-Y1 T, capable of degrading a variety of polysaccharides was isolated from seawater of an oyster farm in the South Sea, Korea. It was found to be aerobic, Gram-negative, non-flagellated, non-gliding and rod-shaped. Strain T-Y1 T grew optimally at 25 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain T-Y1 T belonged to the genus Winogradskyella. Strain T-Y1 T exhibited 16S rRNA gene sequence similarity values of 95.0–96.8 % to the type strains of recognized Winogradskyella species and less than 94.5 % to other validly named species. The chemotaxonomic data concurred with the phylogenetic inference. Strain T-Y1 T contained MK-6 as the predominant menaquinone and anteiso-C 15:0, iso-C 15:0, iso-C 15:1 G and iso-C 16:0 3-OH as the major fatty acids. The major polar lipids of strain T-Y1 T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 36.2 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, enabled strain T-Y1 T to be differentiated from the recognized Winogradskyella species. On the basis of the data presented here, strain T-Y1 T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella multivorans sp. nov. is proposed. The type strain is T-Y1 T (=KCTC 23891 T = CCUG 62216 T). 相似文献
2.
A red-pigmented, Gram-negative, strictly aerobic, rod-shaped bacterium which was motile by gliding, designated strain 1351 T, was isolated from the soil of Lengduo, Tibet in China and subjected to a polyphasic taxonomic analysis. The isolate grows
optimally at 30°C and pH 7. It grows with NaCl tolerated up to 1.5% (optimum, 0.5%). Phylogenetic analysis based on the 16S
rRNA gene sequence shows that strain 1351 T is closely related to members of the family ‘ Cytophagaceae’ closest sequence similarity was observed with similarity with Adhaeribacter terreus (91.8%). The major whole-cell fatty acids are summed feature 4 (containing anteiso-C 17:1 B and/or iso-C 17:1 I, 29.2%), summed feature 3 (containing C 16:1ω6 c and/or C 16:1ω7 c, 13.0%) and iso-C 15:0 (12.0%). The predominant menaquinone of strain 1351 T is menaquinone-7 (MK-7) and the G+C content of the DNA is 46.8 mol%. According to the phylogenetic evidence and phenotypic
data, strain 1351 T is considered to represent a new genus and species of the family ‘ Cytophagaceae’ for which the name Rufibacter tibetensis gen nov., sp. nov. is proposed. The type species is R. tibetensis and the type strain is 1351 T (=CCTCC AB 208084 T = NRRL B-51285 T). 相似文献
3.
The taxonomic position of strain DFH11 T, which was isolated from coastal seawater off Qingdao, People’s Republic of China in 2007, was determined. Strain DFH11 T comprised Gram-negative, motile, strictly aerobic spirilli that did not produce catalase. Comparative 16S rRNA gene sequence
analysis revealed that strain DFH11 T shared ~97.2, 93.3, 91.8, 91.7 and 91.5% sequence similarities with Oleispira antarctica, Spongiispira norvegica, Bermanella marisrubri, Oceaniserpentilla haliotis and Reinekea aestuarii, respectively. DNA–DNA hybridization experiments indicated that the strain was distinct from its closest phylogenetic neighbour,
O. antarctica. The strain grew optimally in 2–3% (w/v) NaCl, at pH 5.0–10.0 (optimally at pH 7.0) and between 0 and 30°C (optimum growth
temperature 28°C). The strain exhibited a restricted substrate profile, with a preference for aliphatic hydrocarbons, that
is consistent with its closest phylogenetic neighbour O. antarctica. Growth of the isolate at different temperatures affected the cellular fatty acid profile. 28°C cultured cells contained
C 16:1ω7c and/or iso-C 15:0 2-OH (50.4%) and C 16:0 (19.2%) as the major fatty acids. However, the major fatty acids of the cells cultured at 4°C were C 16:1ω7c and/or C 16:1ω6c (40.2%), C 16:0 (17.2%) and C 17:1ω8c (10.1%). The G+C content of the genomic DNA was 42.7 mol%. Phylogeny based on 16S rRNA gene sequences together with data
from DNA–DNA hybridization, phenotypic and chemotaxonomic characterization revealed that DFH11 T should be classified as a novel species of the genus Oleispira, for which the name Oleispira
lenta sp. nov. is proposed, with the type strain DFH11 T (=NCIMB 14529 T = LMG 24829 T). 相似文献
4.
A novel bacterial strain designated CJ43T was isolated from fresh water located in Gangwon-do, South Korea, displaying multi-drug resistance. The isolate was Gram-stain-negative, aerobic, orange-pigmented, and rod-shaped. Strain CJ43T grew optimally at 30 °C and pH 7 on R2A agar in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain CJ43T belonged to the genus Pedobacter in the family Sphingobacteriaceae and was most closely related to Pedobacter puniceum HX-22-1 T and P. glucosidilyticus 1-2 T (98.3 and 98.1% sequence similarity). The genome size of strain CJ43T was 3.9 Mb in a single contig with DNA G?+?C content of 34.9%. The genome included 3144 predicted protein-coding genes, as well as 55 tRNA, 9 rRNA and 3 ncRNA genes. The genome also contained 128 putative antibiotic resistance genes, reflecting its phenotypes. The average nucleotide identity values between strain CJ43T and two closely related strains P. puniceum HX-22-1 T and P. glucosidilyticus 1-2 T were 91.0 and 88.7%, respectively. In silico digital DNA-DNA hybridization results between strain CJ43T and the related strains were 42.8 and 38.6%, respectively. The major fatty acids of strain CJ43T were iso-C15:0, iso-C17:0 3-OH, and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c). Strain CJ43T contained phosphatidylethanolamine as the major polar lipid and menaquinone-7 as the sole respiratory quinone. Based on the polyphasic taxonomy data, strain CJ43T represents a novel species of the genus Pedobacter, for which the name Pedobacter aquae sp. nov. is proposed with the type strain CJ43T (=?KACC 21350 T?=?JCM 33709 T).
相似文献
5.
A Gram-positive, non-pigmented, rod-shaped, diazotrophic bacterial strain, designated SC-N012 T, was isolated from rhizosphere soil of sugarcane and was subjected to a polyphasic taxonomic study. The strain exhibited
phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus Bacillus. Sequence analysis of the 16S rRNA gene of SC-N012 T revealed the closest match (98.9% pair wise similarity) with Bacillus clausii DSM 8716 T. However, DNA–DNA hybridization experiments indicated low levels of genomic relatedness (32%) with this strain. The major
components of the fatty acid profile are iso-C 15:0, anteiso-C 15:0, iso-C 17:0 and anteiso-C 17:0. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid. The G+C content of the genomic DNA is 43.0 mol%. The lipids present in strain SC-N012 T are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol and two unknown phospholipids. Their predominant
respiratory quinone was MK-7. Studies of DNA-DNA relatedness, morphological, physiological and chemotaxonomic analyses and
phylogenetic data based on 16S rRNA gene sequencing allowed strain SC-N012 T to be described as members of novel species of the genus Bacillus, for which the name Bacillus rhizosphaerae sp. nov. is proposed. The type strain is SC-N012 T (=DSM 21911 T = NCCB 100267 T). 相似文献
6.
A Gram-stain-negative, non-motile, rod-shaped bacterial strain, JW-64-1 T, capable of degrading methamidophos was isolated from a methamidophos-manufacturing factory in China, and was subjected to
a polyphasic taxonomic investigation. Strain JW-64-1 T produced circular, smooth, transparent, yellow-colored colonies (1.0–2.0 mm) on LB agar after 2 days incubation. It grew
optimally at 25–30°C and pH 7.0 without the presence of NaCl. The G+C content of the total DNA was 63.6 mol%. A phylogenetic
analysis based on 16S rRNA gene sequences showed that strain JW-64-1 T fell within the cluster comprising Luteibacter species. The 16S rRNA gene sequence of strain JW-64-1 T was most closely related to Luteibacter rhizovicinus DSM 16549 T (98.6%), followed by Luteibacter
yeojuensis DSM 17673 T (98.4%) and L. anthropi CCUG 25036 T (98.2%). The major cellular fatty acids of strain JW-64-1 T were iso-C 15:0 (24.1%), iso-C 17:0 (20.2%) and summed feature 9 comprising iso-C 17:1 ω9c and/or C 16:0 10-methyl (20.3%). The major isoprenoid quinine was Q-8 (98%), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol,
phosphoaminolipid, aminolipids-1, aminolipids-2, and phospholipids. The values for DNA–DNA relatedness between strain JW-64-1 T and the closest phylogenetic relatives of L. rhizovicinus and Luteibacter
yeojuensis were 34.8 ± 2.6 and 25.6 ± 3.1%, respectively. On the basis of the phenotypic, chemotaxonomic, DNA–DNA relatedness and phylogenetic
analysis based on the 16S rRNA gene sequences, strain JW-64-1 T represents a novel species of the genus Luteibacter, for which the name Luteibacter jiangsuensis sp. nov. is proposed. The type strain is JW-64-1 T (=CGMCC 1.10133 T = DSM 22396 T). 相似文献
7.
A novel marine bacterium, designated strain CNURIC014 T was isolated from coastal seawater of Jeju Island in Korea. Strain CNURIC014 T formed yellow colonies on marine agar 2216 and the cells were Gram-negative, non-motile, strictly aerobic, rod-shaped. The
temperature, pH and NaCl ranges for growth were 15–37°C, pH 6.0–9.0 and 1.0–7.0% NaCl. Phylogenetic analysis based on 16S
rRNA gene sequences indicated that strain CNURIC014 T was most closely related to Gaetbulibacter marinus and Gaetbulibacter saemankumensis, with a sequence similarity of 95.1% and 94.6%, respectively. The DNA G+C content of the strain was 33.1 mol% and the major
respiratory quinone was menaquinone-6. The major cellular fatty acids were iso-C 15:1 (22.8%), iso-C 15:0 (18.8%), summed feature 3 (iso-C 15:0 2-OH/C 16:1 ω7 c, 12.9%) and iso-C 17:0 3-OH (11.5%). On the basis of phenotypic, phylogenetic, and genotypic data, strain CNURIC014 T represents a novel species within the genus Geatbulibacter, for which the name Gaetbulibacter jejuensis sp. nov. is proposed. The type strain is CNURIC014 T(=KCTC 22615 T =JCM 15976 T). 相似文献
8.
A Gram-staining positive, endospore-forming, motile and rod-shaped bacterial strain, BR-29 T, was isolated from soil from west coast of the Korean peninsula, and its taxonomic position was investigated by a polyphasic
study. Strain BR-29 T grew optimally at around pH 7.5, at 30°C and in the presence of 0.5% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA
gene sequences showed that strain BR-29 T fell into a clade comprising the type strains of Cohnella species, with which it exhibited 16S rRNA gene sequence similarity values of 92.8–96.4%. Strain BR-29 T contained a cell wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The major fatty acids were anteiso-C 15:0, C 16:0 and iso-C 16:0. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, lysylphosphatidylglycerol and two unidentified
phospholipids; a minor amount of phosphatidylglycerol was present. The DNA G+C content was 54.9 mol%. Strain BR-29 T could be differentiated from phylogenetically related Cohnella species by differences in phenotypic characteristics. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain
BR-29 T represents a novel species of the genus Cohnella, for which the name Cohnella
boryungensis sp. nov., is proposed. The type strain is BR-29 T (= KCTC 13735 T = CCUG 59598 T). 相似文献
9.
A Gram-reaction-negative bacterium, strain IMMIB L-12475 T, was isolated from blood cultures of a human with septicaemia. The yellowish orange pigmented strain contained flexirubin pigment. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain IMMIB L-12475 T belonged to the family Flavobacteriaceae, forming a distinct phyletic line that is distantly related (79.1–89.4% sequence similarity) to described genera of this family. Membership to the family was confirmed by a fatty acid profile consisting of branched-chain and 3-hydroxy fatty acids with major amounts of iso-C 17:0 3-OH and iso-C 15:0, by the presence of menaquinone MK-6 as the only respiratory quinone and a polyamine pattern that contained sym-homospermidine as major component. The phospholipids consisted of phosphatidylethanolamine and an unknown phospholipid. The genomic DNA mol% G+C content was 45.6%. The distant phylogenetic position as compared to other representative of the family and the significant phenotypic properties such as pigment composition, morphology, and physiology support the proposal of a novel genus and species Cruoricaptor ignavus gen. nov., sp. nov. The type strain is IMMIB L-12475 T (=DSM 25479 T = CCUG 62025 T). 相似文献
10.
A thermophilic bacterium, designated strain SYSU G04325T, was isolated from a hot spring sediment in Yunnan, China. Polyphasic taxonomic analyses and whole-genome sequencing were used to determine the taxonomic position of the strain. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain SYSU G04325T shows high sequence similarity to Thermoflexibacter ruber NBRC 16677T (86.2%). The strain can be differentiated from other species of the family Thermoflexibacteraceae by its distinct phenotypic and genotypic characteristics. Cells of the strain SYSU G04325T were observed to be aerobic, Gram-stain negative and filamentous. Growth was found to occur optimally at 45 ºC and pH 7.0. In addition, the respiratory quinone was identified as menaquinone-7, while the major fatty acids (>?10%) were identified as iso-C15:0, iso-C17:0 and Summed Feature 9 (iso-C17:1ω9c). The polar lipids detected included phosphatidylethanolamine, three unidentified phospholipids, one unidentified glycolipid, five unidentified aminolipids and four unidentified polar lipids. The G?+?C content of the genomic DNA was determined to be 47.6% based on the draft genome sequence. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU G04325T is concluded to represent a novel species of a novel genus in the family Thermoflexibacteraceae, for which the name Rhodoflexus caldus gen. nov., sp. nov. is proposed. The type strain of Rhodoflexus caldus is SYSU G04325T (=?MCCC 1K06127T?=?KCTC 82848T). 相似文献
11.
A novel bacterium, designated JP1 T, was isolated from soil of Hallasan Mountain in Jeju Island. The isolate was a Gramnegative, aerobic, motile and rod-shaped (0.2–0.4 × 1.2–2.0 μm) bacterium. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain JP1 T was closely related to Dyella koreensis with 97.6% similarity. Growth of strain JP1 T occurred at 10–37°C, pH 5–7 and 0–1% (w/v) NaCl. The genomic DNA G+C content of strain JP1 T was 62.1 mol%. The major fatty acids were iso-C 16:0, iso-C 17:1 ω9c, and iso-C 15:0. The predominant quinone was ubiquinone-8. The major polar lipids of strain JP1 T were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, unidentified aminolipids and unidentified aminophospholipids. The DNA-DNA relatedness values between strain JP1 T and previously reported Dyella species were <10%. Based on phenotypic, genotypic, and phylogenetic distinctness, strain JP1 T represents a novel species in the genus Dyella, for which the name Dyella jejuensis sp. nov. is proposed. The type strain is JP1 T (=KACC 17701 T =JCM 19615 T). 相似文献
12.
An aerobic, Gram-stain negative, rod-shaped, non-motile bacterium, designated as strain HQA918 T, was isolated from an ascidian, Botryllus schlosseri, which was collected from the coast of Weihai in the north of the Yellow Sea, in China. The strain grew optimally at 28–30 °C, at pH values 7.0–8.0, and in the presence of 1.0–3.0% (w/v) sodium chloride (NaCl). A phylogenetic analysis based on 16S rRNA gene sequences showed that strain HQA918 T can be affiliated with the family Flavobacteriaceae in the phylum Bacteroidetes, with 92.7% similarity to its close relatives. The major fatty acids identified were iso-C 15:0, iso-C 15:0 3-OH, and summed feature 3 (iso-C 15:0 2-OH and/or C 16:1ω7c). The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid, and five unidentified polar lipids. The G+C content of the genomic DNA was 44.1 mol%. On the basis of the phylogenetic, genotypic, phenotypic, and chemotaxonomic data, this organism should be classified as a representative of a novel genus, for which the name Ascidiaceibacter gen. nov. is proposed. The type species is Ascidiaceibacter salegens sp. nov. (type strain HQA918 T?=?KCTC 52719 T?=?MCCC 1K03259 T). 相似文献
13.
A novel bacterium B9 T was isolated from tidal flat sediment. Its morphology, physiology, biochemical features, and 16S rRNA gene sequence were
characterized. Colonies of this strain are yellow and the cells are Gram-negative, rod-shaped, and do not require NaCl for
growth. The 16S rRNA gene sequence similarity indicated that strain B9 T is associated with the genus Lysobacter (≤ 97.2%), Xanthomonas (≤ 96.8%), Pseudomonas (≤ 96.7%), and Luteimonas (≤ 96.0%). However, within the phylogenetic tree, this novel strain shares a branching point with the species Luteimonas composti CC-YY255 T (96.0%). The DNA-DNA hybridization experiments showed a DNA-DNA homology of 23.0% between strain B9 T and Luteimonas mephitis B1953/27.1 T. The G+C content of genomic DNA of the type strain is 64.7 mol% (SD, 1.1). The predominant fatty acids are iso-C 11:0, iso-C 15:0, iso-C 16:0, iso-C 17:0, iso-C 17:0
ω9 c, and iso-C 11:0 3-OH. Combined analysis of the 16S rRNA gene sequences, fatty acid profile, and results from physiological and biochemical
tests indicated that there is genotypic and phenotypic differentiation of the isolate from other Luteimonas species. For these reasons, strain B9 T was proposed as a novel species, named Luteimonas aestuarii. The type strain of the new species is B9 T (= KCTC 22048 T, DSM 19680 T). 相似文献
14.
A Gram-stain-positive, aerobic, motile and rod-shaped bacterium, designated RG28T, was isolated from the roots of rice plant collected from paddy fields in Ilsan, South Korea. Cells of the strain were oxidase-negative but catalase-positive. Strain RG28T was found to grow at 10–50 °C (optimum, 25–30 °C), pH 5.0–10.0 (optimum, pH 7.0) and in 1.0–5.0% (w/v) NaCl (optimum, 0%). The cell-wall peptidoglycan contained meso-diaminopimelic acid and the predominant menaquinones were MK-7 and MK-6. The predominant cellular fatty acids were C16:0, iso-C15:0 and anteiso-C15:0. The major polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, four unidentified aminophosphoglycolipids, four unidentified aminophospholipids, two unidentified glycolipids, one unidentified aminoglycolipid and four unidentified lipids. The genomic DNA G?+?C content was 33.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Gottfriedia acidiceleris CBD 119T (98.6%), Gottfriedia solisilvae LMG 18422T (98.5%) and Gottfriedia luciferensis LMG 18422T (98.4%). The average nucleotide identity (ANI) and in silico DNA–DNA hybridization (isDDH) values between strain RG28T and type strains of Gottfriedia species were lower than the cut-offs (≥?95–96% for ANI and?≥?70% for is DDH) required to define a bacterial species. Meanwhile, the strain has the ability to produce indole-acetic acid (40.5 µg/mL). Phylogenetic, physiological and chemotaxonomic data suggested that strain RG28T represented a novel species of the genus Gottfriedia, for which the name Gottfriedia endophyticus sp. nov. is proposed, with the type strain RG28T (=?KCTC 43327T?=?TBRC 15151T). 相似文献
15.
Strain M1-2 T was isolated from the black sand from the seashore of Jeju Island, Republic of Korea and was classified using a polyphasic
taxonomic approach. Strain M1-2 T appeared as Gram-negative, motile rods that could grow in the presence of 1–10% (w/v) NaCl and at temperatures ranging from
4 to 37°C. This isolate has catalase and oxidase activity and hydrolyses aesculin, DNA and l-tyrosine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain M1-2 T belongs to the genus Joostella and is clearly distinct from the other described species of this genus, Joostella marina (type strain En5 T). The 16S rRNA gene sequence similarity level between M1-2 T and J. marina En5 T is 97.2%, and the DNA–DNA relatedness value between the two strains is 23.9%. Strain M1-2 T contains MK-6 as the major menaquinone and iso-C 15:0, summed feature 3 (C 16:1 ω7c and/or iso-C 15:0 2OH) and iso-C 17:0 3OH as major cellular fatty acids. The DNA G + C content is 32.3 mol%. These data suggest that strain M1-2 T should be classified as a novel species, for which the name Joostella atrarenae sp. nov. is proposed. The type strain for the novel species is M1-2 T (= KCTC 23194 T = NCAIM B.002413 T). 相似文献
16.
A Gram-stain-positive, orange-pigmented, rod-shaped and flagellated bacterial strain T12T was isolated from wetland soil in Kunyu Mountain Wetland in Yantai, China. The strain was able to grow at 15–40 °C (optimum 37 °C), at 0.0–9.0% NaCl (optimum 2%, w/v) and at pH 5.5–9.0 (optimum 8.5). A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain T12T is a member of the family Planococcaceae, sharing 97.6% and 97.1% sequence similarity with the type strains of Jeotgalibacillus salarius and Jeotgalibacillus marinus, respectively. Genome-based analyses revealed a genome size of 3,506,682 bp and a DNA G?+?C content of 43.7%. Besides, the genome sequence led to 55.0–74.6% average amino acid identity values and 67.8–74.7% average nucleotide identity values between strain T12T and the current closest relatives. Digital DNA-DNA hybridization of strain T12T with the type strains of Jeotgalibacillus proteolyticus and J. marinus demonstrated 19.0% and 20.3% relatedness, respectively. The chemotaxonomic analysis showed that the sole quinone was MK-7. The predominant cellular fatty acids were iso-C15:0, anteiso-C15:0, C16:1ω7c alcohol and iso-C14:0. The polar lipids consisted of an unidentified aminolipid, phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Based on the polyphasic characterization, strain T12T is considered to represent a novel species, for which the name Jeotgalibacillus aurantiacus sp. nov. is proposed. The type strain is T12T (=?KCTC 43296 T?=?MCCC 1K07171T). 相似文献
17.
A Gram-negative, non-motile, non-spore-forming bacterial strain designated IBFC2009 T was isolated from soil of a bamboo plantation. The strain could grow at 11°C∼39°C, pH 6.0–9.0, and in the presence of 0∼5%
NaCl. Based on 16S rRNA gene sequence analysis, Strain IBFC2009 T belonged to the genus Sphingobacterium and showed the highest sequence similarity of 94.6% ( S. composti T5-12 T) with the type strains within the genus. The major fatty acids were summed feature 3 (iso-C 15:0 2-OH and/or C 16:1
ω7 c, 34.4%), iso-C 15:0 (22.4%), C 16:0 3-OH (15.2%), and iso-C 17:0 3-OH (12.8%). The G+C content of the genomic DNA was 41.0 mol%. According to the phenotypic and genotypic characteristics,
Strain IBFC2009 T should represent a novel species of the genus Sphingobacterium, for which the name Sphingobacterium bambusae sp. nov. is proposed. The type strain is IBFC2009 T (=CCTCC AB 209162 T =KCTC 22814 T). 相似文献
18.
A Gram-negative, aerobic, rod shaped, non-spore-forming bacterial strain, designated Dae08 T, was isolated from sediment of the stream near Daechung dam in South Korea, and was characterized in order to determine its
taxonomic position, using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain Dae08 T belongs to the family Xanthomonadaceae of the Gammaproteobacteria, and is related to Lysobacter brunescens ATCC 29482 T (97.3%). The phylogenetic distances from any other species with validly published names within the genus Lysobacter were greater than 3.7%. The G+C contents of the genomic DNA of strain Dae08 T was 69.3 mol%. The detection of a quinone system with Q-8 as the predominant compound and a fatty acid profile with iso-C 15:0, iso-C 17:1, ω9 c, iso-C 17:0, iso-C 16:0, and iso-C 11:0 3-OH as the major acids supported the affiliation of strain Dae08 T to the genus Lysobacter. DNA-DNA relatedness between strain Dae08 T and its phylogenetically closest neighbour was 28%. On the basis of its phenotypic properties and phylogenetic distinctiveness,
strain Dae08 T (= KCTC 12600 T) should be classified in the genus Lysobacter as the novel species, for which the name Lysobacter daecheongensis sp. nov. is proposed. 相似文献
19.
An aerobic, Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, and light pink-colored bacterial strain, designated TS19T, was isolated from a sand sample obtained from a coastal sand dune after exposure to 3 kGy of gamma radiation. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the isolate was a member of the genus Hymenobacter and was most closely related to H. wooponensis WM78T (98.3% similarity). Strain TS19T and H. wooponensis showed resistance to gamma radiation with D10 values (i.e., the dose required to reduce the bacterial population by tenfold) of 7.3 kGy and 3.5 kGy, respectively. The genome of strain TS19T consists of one contig with 4,879,662 bp and has a G?+?C content of 56.2%. The genome contains 3,955 protein coding sequences, 44 tRNAs, and 12 rRNAs. The predominant fatty acids of strain TS19T were iso-C15:0, summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B), summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), and C16:1 ω5c. The major polar lipids were phosphatidylethanolamine, and one unidentified aminophospholipid. The main respiratory quinone was menaquinone-7. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain TS19T represents a novel species, for which the name Hymenobacter taeanensis sp. nov. is proposed. The type strain is TS19T (=?KCTC 72897T?=?JCM 34023T). 相似文献
20.
Strain XBU10T was isolated from a soil sample of a sunflower plot in Inner Mongolia, China. The isolate was a Gram-stain-negative, aerobic, non-motile, rod-shaped bacterium, and its colonies were bright yellow in colour. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XBU10T belonged to the genus Luteimonas of the family Lysobacteraceae and was most closely related to Luteimonas panaciterrae Gsoil 068T (97.8%), Luteimonas marina FR1330T (97.6%), Luteimonas aquatica RIB1-20T (97.4%) and Luteimonas huabeiensis HB2T (97.2%). Growth occurred at 4–40 °C (optimum, 28–30 °C), with 0–5.0% (w/v) NaCl (optimum, 0.5%) and at pH 6.0–10.0 (optimum, pH 7.0???8.0). The chemotaxonomic characteristics of strain XBU10T, which had Q-8 as its predominant quinone and iso-C17:1 ω9c, iso-C15:0, iso-C17:0 and iso-C16:0 as its major fatty acids, were consistent with classification in the genus Luteimonas. The polar lipid profile of strain XBU10T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminophospholipids and three unidentified polar lipids. The genome of strain XBU10T was 4.17 Mbp with a G?+?C content of 69.9%. Its genome sequence showed genes encoding alkaline phosphatase and catalase. Protein-coding genes related to carbohydrate-active enzymes were also observed. Average nucleotide identity (ANI) values between XBU10T and other species of the genus Luteimonas were found to be low (ANIm?<?88.0%, ANIb?<?85.0% and OrthoANIu?<?85.0%). Furthermore, digital DNA-DNA hybridization (dDDH) and average amino acid identity (AAI) values between strain XBU10T and the closely related species ranged from 20.3 to 28.9% and from 64.2 to 82.3%, respectively. Based on the results of our phylogenetic, phenotypic, genotypic and chemotaxonomic analyses, it is concluded that strain XBU10T represents a novel species within the genus Luteimonas, for which the name Luteimonas viscosa sp. nov. is proposed. The type strain is XBU10T (=?CGMCC 1.12158T?=?KCTC 23878T). 相似文献
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