共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
4.
5.
Stuart C Andrews Michelle D Wood Simon J Tunster Sheila C Barton M Azim Surani Rosalind M John 《BMC developmental biology》2007,7(1):53
Background
Cdkn1c encodes an embryonic cyclin-dependant kinase inhibitor that acts to negatively regulate cell proliferation and, in some tissues, to actively direct differentiation. This gene, which is an imprinted gene expressed only from the maternal allele, lies within a complex region on mouse distal chromosome 7, called the IC2 domain, which contains several other imprinted genes. Studies on mouse embryos suggest a key role for genomic imprinting in regulating embryonic growth and this has led to the proposal that imprinting evolved as a consequence of the mismatched contribution of parental resources in mammals. 相似文献6.
Zhang J Chen R Xiao J Qian C Wang T Li H Ouyang B Ye Z 《Journal of plant research》2007,120(6):671-678
The entire (e) locus of tomato (Solanum lycopersicum L.) controls leaf morphology. Dominant E and recessive e allele of the locus produce pinnate compound and complex reduced leaves. Previous research had indicated that SlIAA9, an Aux/IAA gene, was involved in tomato leaf morphology. Down-regulation of SlIAA9 gene by antisense transgenic method decreased the leaf complex of tomato and converted tomato compound leaves to simple leaves.
The leaf morphology of these transgenic lines was similar with leaf morphology of tomato entire mutant. In this paper, we report that a single-base deletion mutation in the coding region of SlIAA9 gene results in tomato entire mutant phenotypes. 相似文献
7.
8.
Wu G Wu Y Xiao L Li X Lu C 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(4):491-499
The fatty acid elongase 1 (FAE1) gene is a key gene in the erucic acid biosynthesis in rapeseed. The complete coding sequences of the FAE1 gene were isolated separately from eight high and zero erucic acid rapeseed cultivars (Brassica napus L.). A four base pair deletion between T1366 and G1369 in the FAE1 gene was found in a number of the cultivars, which leads to a frameshift mutation and a premature stop of the translation
after the 466th amino acid residue. This deletion was predominantly found in the C-genome and rarely in the A-genome of B. napus. Expression of the gene isoforms with the four base pair deletion in a yeast system generated truncated proteins with no
enzymatic activity and could not produce very long chain fatty acids as the control with an intact FAE1 gene did in yeast cells. In the developing rape seeds the FAE1 gene isoforms with the four base pair deletion were transcribed normally but failed to translate proteins to form a functional
complex. The four base pair deletion proved to be a mutation responsible for the low erucic acid trait in rapeseed and independent
from the point mutation reported by Han et al. (Plant Mol Biol 46:229–239, 2001).
Gang Wu, Yuhua Wu contribute equally to this article. 相似文献
9.
10.
XiuXia He ChongWei Jin GuiXin Li GuangYi You XuePing Zhou ShaoJian Zheng 《中国科学C辑(英文版)》2008,51(5):402-409
Virus-induced gene silencing (VIGS) is potentially an attractive reverse-genetics tool for studies of plant gene function,
but whether it is effective in silencing mineral nutritional-related genes in roots has not been demonstrated. Here we report
on an efficient VIGS system that functions in tomato roots using a modified viral satellite DNA (DNAmβ) associated with Tomato
yellow leaf curl China virus (TYLCCNV). A cDNA fragment of the ferric chelate reductase gene (FRO1) from tomato was inserted into the DNAmβ vector. Tomato roots agro-inoculated with DNAmβ carrying both a fragment of FRO1 and TYLCCNV used as a helper virus exhibited a significant reduction at the FRO1 mRNA level. As a consequence, ferric chelate reductase activity, as determined by visualization of the pink FeBPDS3 complex was significantly decreased. Our results clearly demonstrated that VIGS system can be employed to investigate gene
function associated with plant nutrient uptake in roots. 相似文献
11.
12.
Xiaoping Chen Zhangying Wang Jianhua Wang Maoyan Wang Li Zhao Guoying Wang 《Plant Cell, Tissue and Organ Culture》2007,88(1):11-20
ADP-glucose pyrophosphorylase (AGPase) represents a key regulatory step in starch synthesis. A 0.9 kb of 5′ flanking region
preceding Brittle2 gene, encoding the small subunit of maize endosperm AGPase, was cloned from maize genome and its expression pattern was studied
via the expression of β-glucuronidase (GUS) gene in transgenic tobacco. Analysis of GUS activities showed that the 0.9 kb
fragment flanking Brittle2 gene was sufficient for driving the seed-preferred expression of the reporter gene. The activity of the 0.9 kb 5′ flanking
fragment was compared with that of the tandem promoter region from a zein gene (zE19, encoding a maize 19 kDa zein protein). The results indicated that both promoters were seed-preferred in a dicotyledonous
system as tobacco and the activity of zE19 promoter was three to fourfold higher than that of the 0.9 kb fragment flanking Brittle2 gene in transgenic tobacco seeds. At the same time, zE19-driven GUS gene expressed earlier than Brittle2 promoter during seed development. Histochemical location of GUS activity indicated that both promoters showed high expression
in embryos, which is different from similar promoters tested in maize. 相似文献
13.
14.
Background
Transferrin binding protein B (tbpB), an outer membrane lipoprotein, is required for the acquisition of iron from human transferrin. Two tbpB families have been documented in Neisseria meningitidis: an isotype I tbpB gene of 1.8 kb and an isotype II tbpB gene of 2.1 kb, the former expressed by meningococci in the disease-associated ST-11 clonal complex and the latter found among meningococci belonging to the hyper-invasive clonal complexes including ST-8, ST-18, ST-32, ST-41/44 as well as N. gonorrhoeae isolates. The origin of the isotype I tbpB gene is unknown, however several features in common with non-pathogenic Neisseria and the ST-11 clonal complex N. meningitidis isolate FAM18 have been documented leading to the hypothesis that the isotype I tbpB gene may also be shared between non-pathogenic Neisseria and ST-11 meningococci. As a result, the diversity of the tbpB gene was investigated in a defined collection of Neisseria species. 相似文献15.
16.
The lactose operon in Escherichia coli was the first known gene regulatory network, and it is frequently used as a prototype for new modeling paradigms. Historically, many of these modeling frameworks use differential equations. More recently, Stigler and Veliz-Cuba proposed a Boolean model that captures the bistability of the system and all of the biological steady states. In this paper, we model the well-known arabinose operon in E. coli with a Boolean network. This has several complex features not found in the lac operon, such as a protein that is both an activator and repressor, a DNA looping mechanism for gene repression, and the lack of inducer exclusion by glucose. For 11 out of 12 choices of initial conditions, we use computational algebra and Sage to verify that the state space contains a single fixed point that correctly matches the biology. The final initial condition, medium levels of arabinose and no glucose, successfully predicts the system’s bistability. Finally, we compare the state space under synchronous and asynchronous update and see that the former has several artificial cycles that go away under a general asynchronous update. 相似文献
17.
Demirev AV Khanal A Hanh NP Nam KT Nam DH 《Journal of microbiology (Seoul, Korea)》2011,49(3):407-412
Acyl-CoA carboxylases (ACC) are involved in important primary or secondary metabolic pathways such as fatty acid and/or polyketides
synthesis. In the 62 kb fragment of pccB gene locus of Streptomyces toxytricini producing a pancreatic inhibitor lipstatin, 3 distinct subunit genes of presumable propionyl-CoA carboxylase (PCCase) complex,
assumed to be one of ACC responsible for the secondary metabolism, were identified along with gene for a biotin protein ligase
(Bpl). The subunits of PCCase complex were a subunit (AccA3), P subunit (PccB), and auxiliary ɛ subunit (PccE). In order to
disclose the involvement of the PCCase complex in secondary metabolism, some biochemical characteristics of each subunit as
well as their complex were examined. In the test of substrate specificity of the PCCase complex, it was confirmed that this
complex showed much higher conversion of propionyl-CoA rather than acetyl-CoA. It implies the enzyme complex could play a
main role in the production of methylmalonyl-CoA from propionyl-CoA, which is a precursor of secondary polyketide biosynthesis. 相似文献
18.
WonKyung Kang 《中国病毒学》2009,24(4):315-322
Viruses including baculoviruses are obligatory parasites, as their genomes do not encode all the proteins required for replication.
Therefore, viruses have evolved to exploit the behavior and the physiology of their hosts and often coevolved with their hosts
over millions of years. Recent comparative analyses of complete genome sequences of baculoviruses revealed the patterns of
gene acquisitions and losses that have occurred during baculovirus evolution. In addition, knowledge of virus genes has also
provided understanding of the mechanism of baculovirus infection including replication, species-specific virulence and host
range. The Bm8 gene of Bombyx mori nucleopolyhedrovirus (NPV) and its homologues are found only in group I NPV genomes. The Autographa californica NPV Ac16 gene is a homologue of Bm8 and, encodes a viral structural protein. It has been shown that Bm8/Ac16 interacts with
baculoviral and cellular proteins. Bm8/Ac16 interacts with baculoviral IE1 that is facilitated by coiled coil domains, and
the interaction with IE1 is important for Bm8 function. Ac16 also forms a complex with viral FP25 and cellular actin and associates
with membranes via palmitoylation. These data suggested that this gene family encodes a multifunctional protein that accomplishes
specific needs of group I NPVs.
相似文献
19.
T. A. Bukharina V. P. Golubyatnikov I. V. Golubyatnikov D. P. Furman 《Russian Journal of Developmental Biology》2012,43(1):49-53
Morphogenesis of drosophila macrochaete functioning as mechanoreceptors includes several steps, each of which has their own
genetic support described in terms of gene nets. Mechanoreceptor develops from one parental cell (Sensory Organ Precursor
cell—SOP cell), the determination of which has a critical role in macrochaete development. The highest content of AS-C proneural
proteins with respect to surrounding cells that initiate a neural way of cellular development and by means of it mechanoreceptor
morphogenesis is typical for SOP cell. The key object of gene net providing parental cell determination consists of gene complex
achaete-scute (AS-C). This complex activity is controlled by central regulatory contour (CRC). Besides AS-C, CRC includes the following genes: hairy, senseless (sens), charlatan (chn), scratch (scrt), daughterless (da), extramacrochaete (emc), and groucho (gro). The system of direct relation and feedback and induction and repression relations between CRC components are realized via
the coding by these genes proteins. A mathematical model of CRC functioning as a regulator of proneural AS-C protein content
in SOP cell determining successful passing of the main phase of morphogenesis of D. melanogaster mechanoreceptor is discussed. 相似文献
20.
N. Y. Martynova F. M. Eroshkin A. G. Zaraisky 《Russian Journal of Bioorganic Chemistry》2018,44(3):362-365