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1.
Boron adsorption by maize cell walls 总被引:1,自引:0,他引:1
Boron adsorption by cell walls isolated from corn (Zea mays) roots was investigated as a function of solution pH and ionic strength. Adsorption increased with increasing solution pH from pH 4.5 to 10, exhibited an adsorption maximum at pH 10–10.5, and decreased with increases in pH above 10.5. Boron adsorption increased with increasing solution ionic strength indicating the formation of strong inner-sphere surface complexes. A surface complexation model, the constant capacitance model was well able to describe the B adsorption data, optimizing two B surface complexes and the dissociation constant for the surface functional group, XOH. The large absolute value of the dissociation constant is consistent with phenolic functional groups. 相似文献
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3.
Summary Maize (Zea mays L. cv. Ganga-2) plants were grown in pot culture on a loamy alluvial soil of Lucknow district (India) alkalinized to graded
levels of ESP (Exchangeable Sodium Percentage) ranging from 15.5 to 55.3. Before sowing maize seeds the soil was fertilised
with NPK, Fe, Mn and Cu. At and above ESP 34 Zn-deficiency symptoms first appeared at 30 days. The symptoms gradually became
pronounced with increase in age and at 60 days they were found even at ESP 15.5. The severity of symptoms was related to increase
in sodicity. Alkalinization of soils depressed available soil Zn and tissue Zn and increased tissue ratios of Na/Zn and P/Zn.
It also decreased the total plant content of Zn, Fe, Mn, Cu and even Na. Increase in soil sodicity increased both tissue concentration
and total content of P in plants upto ESP 34 beyond which it decreased it. Among different extractants, 0.1N HCl, DTPA pH 7.3 and EDTA-(NH4)2 CO3 pH 8.6, for measuring available soil Zn the latter showed best correlations with soil ESP (−), tissue P (−), P/Zn ratio (−),
dry matter yield (+) and tissue Zn (+). Tissue Zn was related to yield (+), tissue Na (−) and soil ESP (−). Mild, moderate,
severe and very severe Zn deficiency in maize was induced by soil ESP levels, 18, 25, 33 and 45, respectively. 相似文献
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5.
Boron deficiency increases putrescine levels in tobacco plants 总被引:5,自引:0,他引:5
Camacho-Cristóbal JJ Maldonado JM Gonzláez-Fontes A 《Journal of plant physiology》2005,162(8):921-928
Polyamine concentrations were determined in leaves and roots of tobacco plants (Nicotiana tabacum L.) subjected to a short-term boron deficiency. A decrease in the growth of shoots and, especially, roots was found under this mineral deficiency. Boron deficiency did not lead to a significant decrease in leaf or root ion concentrations when compared to control treatment; however, as expected, leaf boron concentration was lower in boron-deficient plants in comparison to the control. In leaves, the levels of free putrescine and spermidine were similar in both treatments. In roots, a short-term boron deficiency caused an increase in free putrescine. Moreover, boron-deficient plants had higher conjugated polyamine concentration than boron-sufficient plants, which was especially evident for conjugated putrescine in leaves. A possible link between boron and polyamine levels is proposed and discussed. 相似文献
6.
Manganese deficiency in maize affects pollen viability 总被引:2,自引:0,他引:2
Maize (Zea mays L. cv. G2) was grown with 0.55 mg L–1 (sufficient), or 0.0055 mg L–1 (deficient) manganese in sand. Manganese-deficient plants developed visible deficiency symptoms and showed poor tasseling and delayed anther development. Compared to Mn-sufficient plants, Mn-deficient plants produced fewer and smaller pollen grains with reduced cytoplasmic contents. Manganese deficiency reduced in vitro germination of pollen grains significantly. Ovule fertility was not significantly affected by Mn. But in Mn-deficient plants seed-setting and development was reduced significantly. 相似文献
7.
Fort DJ 《Biological trace element research》2002,85(2):157-169
Processes of oocyte maturation that may be affected by boron (B) deficiency were studied to potentially determine a possible
biochemical role of B in the Xenopus laevis oocyte. More specifically, the Xenopus oocyte membrane progesterone receptor (OMPR) in B-deficient oocytes was characterized by evaluating progesterone affinity
for the OMPR and OMPR responsiveness to progesterone stimulation. The responsiveness of B-deficient oocytes to microinjection
of a purified oocyte cytoplasmic fraction (OCF) from B-adequate oocytes was also studied to evaluate which aspects of the
maturation process were affected by B deficiency. Results suggested that B deficiency resulted in incomplete oocyte maturation
and that maturation could not be induced by the administration of exogenous progesterone. Progesterone successfully induced
germinal vesicle breakdown (GVBD) in oocytes from females fed a B-supplemented diet (+B) and females administered a traditional
diet of beef liver and lung (B adequate). Addition of exogenous B to the -B oocytes increased the rate of progesterone-induced
GVBD slightly. The B-deficient X. laevis oocytes were capable of undergoing GVBD when endogenously stimulated by microinjected purified B-adequate OCF. These results
indicated that the inability of the B-deficient oocytes to undergo GVBD was not associated with the cytoplasmic induction
process specifically, but possibly in the progesterone receptor or signal transduction pathways. Radio-binding studies found
that progesterone binding to the B-deficient OPMR was greatly reduced compared to B-adequate or B-supplemented OMPR. Moreover,
washout studies determined that progesterone binding to the OMPR in B-deficient oocytes was more transient than the B adequate
or +B oocytes. 相似文献
8.
Sharma S 《Journal of plant physiology》2007,164(10):1261-1267
This paper explores the effects of high light stress on Fe-deficient plants. Maize (Zea mays) plants were grown under conditions of Fe deficiency and complete nutrition. Attached, intact leaves of Fe-deficient and control plants were used for gas exchange experiments under suboptimal, optimal and photoinhibitory illumination. Isolated chloroplasts were used to study photosynthetic electron transport system, compromised by the induction of Fe deficiency. The reaction centers of PS II (measured as reduction of Q, the primary electron acceptor of P 680) and PS I (measured as oxidation of P 700) were estimated from the amplitude of light induced absorbance change at 320 and 700 nm, respectively. Plants were subjected to photoinhibitory treatment for different time periods and isolated chloroplasts from these plants were used for electron transport studies. Carbon dioxide fixation in control as well as in Fe-deficient plants decreased in response to high light intensities. Total chlorophyll, P 700 and Q content in Fe-deficient chloroplasts decreased, while Chl a/b ratio and Q/P 700 ratio increased. However, electron transport through PS II suffered more after photoinhibitory treatment as compared to electron transport through PS I or whole chain. Electron transfer through PS I+PS II, excluding the water oxidation complex showed a decrease in Fe-deficient plants. However, electron transport through this part of the chain did not suffer much as a result of photoinhibition, suggesting a defect in the oxidising side of PS II. 相似文献
9.
Maize (Zea mays L.) is a betaine-accumulating species, but certain maize genotypes lack betaine almost completely; a single recessive gene has been implicated as the cause of this deficiency (D Rhodes, PJ Rich [1988] Plant Physiol 88: 102-108). This study was undertaken to determine whether betaine deficiency in diverse maize germplasm is conditioned by the same genetic locus, and to define the biochemical lesion(s) involved. Complementation tests indicated that all 13 deficient genotypes tested shared a common locus. One maize population (P77) was found to be segregating for betaine deficiency, and true breeding individuals were used to produce related lines with and without betaine. Leaf tissue of both betaine-positive and betaine-deficient lines readily converted supplied betaine aldehyde to betaine, but only the betaine-containing line was able to oxidize supplied choline to betaine. This locates the lesion in betaine-deficient plants at the choline → betaine aldehyde step of betaine synthesis. Consistent with this location, betaine-deficient plants were shown to have no detectable endogenous pool of betaine aldehyde. 相似文献
10.
Two pot experiments at the Plant Environment Laboratory (PEL), Reading, UK investigated sterility, boron (B) accumulation
and B partitioning of wheat cultivars grown with limited B in the growing medium. The first experiment evaluated nine cultivars
of spring wheat with diverse field responses to low available soil B, supplied with or without 20 μM B. A second experiment
examined the response of a susceptible (SW-41) and a tolerant (Fang-60) cultivar to B-deficiency. These cultivars were supplied
with either 20 μM B from sowing to flag leaf emergence and no added B thereafter, or 20 μM B from sowing to maturity. When
B was not supplied in the nutrient solution, the number of grains ranged from 4 per ear (cv. BL-1135) to 32 per ear (cv. BL-1249)
and sterility of competent florets ranged from 39% to 93%. Boron concentration in the flag leaf at anthesis did not differ
greatly when the growing medium contained limited B, but differences between cultivars were evident when B was unlimited.
Tolerance of B-deficiency was not related to the B concentration in the flag leaf. Some cultivars produced viable pollen and
set grains while others failed to do so at similar B concentrations in the flag leaf. The two contrasting cultivars did not
differ much in their pattern of B partitioning when B supply was restricted from flag leaf emergence onwards. Similarly, little
evidence was found that the tolerant cultivars translocated B from their leaves, roots or stems when the supply in the growing
medium was restricted. The proportion of total B partitioned in different organs was the same irrespective of B supply and
cultivar. On average, leaves contained 68% of the total B content in the whole plant compared to 16% in the roots, 10% in
the ears and only 6% in the stems. Tolerant or susceptible cultivars of wheat could not be distinguished based on the B concentration
and B content of the flag leaf.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
11.
Boron deficiency in lentil: Yield loss and geographic distribution in a germplasm collection 总被引:2,自引:0,他引:2
Srivastava S.P. Bhandari T.M.S. Yadav C.R. Joshi M. Erskine W. 《Plant and Soil》2000,219(1-2):147-151
In a programme to improve the production of lentil (Lens culinaris Medikus), germplasm was introduced into Nepal. However, the evaluation of exotic germplasm in the Chitwan region of Nepal revealed a striking micronutrient deficiency problem. Landraces from Nepal had no deficiency symptoms, whereas 82% out of 494 exotic accessions showed severe chlorosis and stunting. Boron (B) deficiency was revealed as the primary cause of the problem and the first limiting nutrient, because an exotic genotype, which exhibited the symptoms, yielded only 103 kg seed/ha, but produced 1367 kg seed ha with the application of 0.5 kg boric acid/ha, a 13-fold yield increase. There was also a significant but lower response in yield (20%) to applied Zn. Exotic germplasm from various countries showed differences in B-deficiency symptoms. Accessions from Syria, representative of the Mediterranean region where lentil originated, all exhibited B-deficiency symptoms. Accessions without B-deficiency symptoms were mainly from Bangladesh (37% accessions free of B deficiency). In a subsequent trial to estimate yield losses, landraces from Nepal, which exhibited no deficiency symptoms, were B efficient and gave a mean seed yield of 1173 kg/ha. In contrast, 10 exotic lines exhibited severe symptoms and gave no seed yield. In summary, the study revealed that, in soil low in B, striking genetic differences in B efficiency associated with geographic origin are apparent which may lead to complete yield loss in B-inefficient lentil germplasm. 相似文献
12.
Growth analysis of maize field crops under phosphorus deficiency 总被引:6,自引:0,他引:6
Biomass accumulation by crops depends both on light interception by leaves and on the efficiency with which the intercepted
light is used to produce dry matter. Our aim was to identify which of these processes were affected for maize (Zea Mays L., cv Volga) field crops grown under phosphorus (P) deficiency, and assess their relative importance. In this paper, the
effects of P deficiency on leaf appearance, leaf elongation rate, final individual leaf area and leaf senescence were studied.
The experimental work was carried out in 1995–1977 on a long-term P fertilisation trial located on a sandy soil in the south-west
of France. Three P fertilisation regimes have been applied since 1972: no-P (P0 treatment) and different rates of P fertiliser
(P1.5:1.5 times the grain P export and P3:3 times the grain P export). These fertilisation regimes have led to contrasted
levels of soil P supply, with the P0 treatment being limiting for growth. Very few differences were observed about leaf growth
between the P1.5 and P3 treatments. Conversely, the leaf area index (LAI) was significantly reduced in the P0 treatment, especially
during the first phases of the crop cycle (up to −60% between the 7- and 14-visible leaves). This effect gradually decreased
over time. The lower LAI in P0 treatment was due to two main processes affecting the leaf growth. The final number of leaves
per plant and leaf senescence were only slightly modified by P deficiency. Conversely, leaf appearance was delayed during
the period between leaf 4 and leaf 9. The value of the phyllochron increased from 47 °C days in the P1.5 treatment to 65 °C
days in the P0 treatment. Leaf elongation rates during the quasi-linear phase of leaf expansion were significantly reduced
for lower leaves of P0 plants. The final size of leaves L2–L12 was reduced. On the opposite, leaf elongation duration was
not greatly affected by P treatments. Before the emergence of leaf 9, the reduction of individual leaf size was the main factor
responsible for the reduced LAI in the P0 treatment. After this stage, the delayed leaf appearance accounted for a great part
of the reduced LAI in the P0 treatment.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
13.
Evidence is presented to show that the Sh locus specifies sucrose synthetase in the developing endosperm of maize. The sh/sh/sh endosperm possesses less than 10% sucrose synthetase activity as compared to the normal Sh/sh/sh endosperm. The residual enzyme activity in five independently derived mutant genotypes is attributable to a protein molecule of different electrophoretic and immunochemical specificities that is presumably independent of the sh locus. Sucrose synthetase activity in the embryo in both the genotypes is electrophoretically indistinguishable from the one present in the mutant endosperm. Mutant endosperm has a reduced starch content as compared to the normal. This observation constitutes genetic evidence supporting a critical role for sucrose synthetase in starch biosynthesis. 相似文献
14.
Zinc deficiency decreased pollen viability in maize (Zea mays L. cv. G2) grown in sand culture. On restoring normal zinc supply to zinc-deficient plants before the pollen mother cell stage of anther development, the vegetative yield of plants and pollen fertility could be recovered to a large extent, but the recovery treatment was not effective when given after the release of microspores from the tetrads. If zinc deficiency was induced prior to microsporogenesis it did not significantly affect vegetative yield and ovule fertility, but decreased the fertility of pollen grains, even of those which visibly appeared normal. If the deficiency was induced after the release of microspores from the tetrads, not only vegetative yield and ovule fertility but pollen fertility also remained unaffected. 相似文献
15.
Marie Kummerová 《Biologia Plantarum》1986,28(4):270-274
The effect of phosphorus deficiency on acid phosphatase activity in the apical, middle and basal parts of the root of maize plants was followed. The supernatant obtained by centrifuging the homogenate of plant tissue at 1500 ×g was further centrifuged at 18 000 ×g, the sediment being marked as fraction II and the supernatant as fraction III. The results obtained document the fact that acid phosphatase activity of the two fractions of all analyzed root segments was higher in plants cultured in nutrient medium without phosphate than in those containing phosphorus in nutrient medium. In most cases this difference was significant to highly significant. The results of experiments proved unambiguously a higher enzymatic activity in all root segments in fraction III than in fraction II. In fraction III the highest acid phosphatase activity was found in the apical part, in fraction II in the basal part of the root. 相似文献
16.
Boron deficiency causes accumulation of chlorogenic acid and caffeoyl polyamine conjugates in tobacco leaves 总被引:5,自引:0,他引:5
Camacho-Cristóbal JJ Lunar L Lafont F Baumert A González-Fontes A 《Journal of plant physiology》2004,161(7):879-881
The effects of boron (B) deficiency on carbohydrate concentrations and the pattern of phenolic compounds were studied in leaves of tobacco plants (Nicotiana tabacum L.). Plants grown under B deficiency showed a notable increase in leaf carbohydrates and total phenolic compounds when compared to controls. The qualitative composition of phenolics was analyzed by HPLC-mass spectrometry. The level of caffeate conjugates (i.e., chlorogenic acid) increased in B-deficient plants. In addition, the accumulation of two caffeic acid amides (N-caffeoylputrescine and putative dicaffeoylspermidine) was observed. 相似文献
17.
Boron (B) is an essential microelement for the growth and development of plants. B-deficient radish plants grew slowly compared to B-sufficient controls. Soluble B and cell wall-bound B decreased in young leaves on removal of B from culture medium. In old leaves, B deficiency reduced soluble B content but there was no significant effect on cell wall-bound B content compared to controls. The mesophyll cells in the middle of leaves were enlarged abnormally and had greater cell wall thickness under B-deficient conditions. B deficiency reduced the stomata frequency, inhibited the stomata aperture, and guard cells had thickened cell walls. B-starved leaves showed decreased photosynthesis and stomatal conductance. These indicate that B deficiency could interfere with cell wall development, especially irregular guard cell walls as a result of B deficiency severely affected the rhythmic stomatal closing and opening, preventing the normal functioning of stomata. Correspondingly, photosynthesis was indirectly affected, and plant growth decreased. 相似文献
18.
Cloning and characterization of maize miRNAs involved in responses to nitrogen deficiency 总被引:1,自引:0,他引:1
Although recent studies indicated that miRNAs regulate plant adaptive responses to nutrient deprivation, the functional significance of miRNAs in adaptive responses to nitrogen (N) limitation remains to be explored. To elucidate the molecular biology underlying N sensing/signaling in maize, we constructed four small RNA libraries and one degradome from maize seedlings exposed to N deficiency. We discovered a total of 99 absolutely new loci belonging to 47 miRNA families by small RNA deep sequencing and degradome sequencing, as well as 9 new loci were the paralogs of previously reported miR169, miR171, and miR398, significantly expanding the reported 150 high confidence genes within 26 miRNA families in maize. Bioinformatic and subsequent small RNA northern blot analysis identified eight miRNA families (five conserved and three newly identified) differentially expressed under the N-deficient condition. Predicted and degradome-validated targets of the newly identified miRNAs suggest their involvement in a broad range of cellular responses and metabolic processes. Because maize is not only an important crop but is also a genetic model for basic biological research, our research contributes to the understanding of the regulatory roles of miRNAs in plant adaption to N-deficiency stress. 相似文献
19.
Organ-specific invertase deficiency in the primary root of an inbred maize line 总被引:3,自引:0,他引:3
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An organ-specific invertase deficiency affecting only the primary root system is described in the Oh 43 inbred maize (Zea mays). Invertases (acid and neutral/soluble and insoluble) were assayed in various tissues of hybrid (NK 508) and inbred (Oh 43, W 22) maize lines to determine the basis for an early report that Oh 43 root tips were unable to grow on sucrose agar (27). Substantial acid invertase activity (7.3 to 16.1 micromoles of glucose per milligram of protein per hour) was evident in extracts of all tissues tested except the primary root system of Oh 43. This deficiency was also evident in lateral roots arising from the primary root. In contrast, morphologically identical lateral roots from the adventitious root system had normal invertase levels. These results suggest that ontogenetic origin of root tissues is an important determinant of invertase expression in maize. Adventitious roots (including the seminals) arise above the scutellar node and are, therefore, of shoot origin. The Oh 43 deficiency also demonstrated that invertase activity was not essential for maize root growth. Sucrose synthase was active in extracts from all root apices and theoretically provided the only available avenue for sucrose degradation in primary root tips of Oh 43. The deficiency described here will provide a useful avenue of investigation into the expression and significance of root invertase. 相似文献