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1.
The objective of this study was to determine the effects of plant growth regulator (PGR) (no PGR, trinexapac-ethyl, and paclobutrazol) and N fertilizer (zero N, an average of 37 kg N ha?1 month?1, 6 and 12 kg N ha?1 week?1) on soil organic C (SOC) and soil N in creeping bentgrass (Agrostis stolonifera L.) fairway turf. After 4 years of field experiments soil samples were obtained from soil depths of 0–2.5, 2.5–5, 5–7.5, 7.5–10, 10–15, 15–20, and 20–30 cm. Soil bulk density, SOC, total N, NO 3 ? –N, and NH 4 + –N concentrations were determined. Paclobutrazol and trinexapac-ethyl application increased SOC. The 37 kg N ha?1 month?1 application increased SOC at the 0–2.5 cm depth with both PGRs. When paclobutrazol was used, N fertilizer always increased SOC; however, the greatest increase was observed with the 12 kg N ha?1 week?1 application when compared to other rates, inversely related to the NH 4 + –N concentration. Nitrogen application increased soil total N and NO 3 ? –N in the upper three depths. The application of PGRs and N fertilizer to creeping bentgrass fairway turf is an effective strategy for promoting C sequestration.  相似文献   

2.
Nitrogen cycling in forest soils across climate gradients in Eastern China   总被引:9,自引:0,他引:9  
A 15N tracing study was carried out to investigate the potential gross nitrogen (N) dynamics in thirteen forest soils in Eastern China ranging from temperate to tropical zones (five coniferous forests, six deciduous broad-leaf forests, one temperate mixed forest, one evergreen broad-leaf forests ecosystems), and to identify the major controlling factors on N cycling in these forest ecosystems. The soil pH ranged from 4.3 to 7.9 and soil organic carbon (SOC) ranged from 6.6 g?kg?1 to 83.0 g?kg?1. The potential gross N transformation rates were quantified by 15N tracing studies where either the ammonium or nitrate pools were 15N labeled in parallel treatments. Gross mineralization rates ranged from 0.915 μg N g?1 soil day?1 to 2.718 μg N g?1 soil day?1 in the studied forest soils. The average contribution of labile organic-N (M Nlab ) to total gross mineralization (M Nrec +M Nlab ) was 86% (58% to 99%), indicating that turnover of labile organic N plays a dominant role in the studied forest ecosystems. The gross mineralization rates in coniferous forest soils were significantly lower (ranging between 0.915 and 1.228 μg N g?1 soil day?1) compared to broad-leaf forest soils (ranging from 1.621 to 2.718 μg N g?1 soil day?1) (p?<?0.01). Thus, the dominant vegetation may play an important role in regulating soil N mineralization. Nitrate production (nitrification) occurred via two pathways, oxidation of NH 4 + and organic N the forest soils. Correlations with soil pH indicated that this is a key factor controlling the oxidation of NH 4 + and organic N in theses forest ecosystems. NH 4 + oxidation decreased with a decline in pH while organic N oxidation increased. The climatic conditions (e.g. moisture status) at the various sites governed the NO 3 ? -N consumption processes (dissimilatory NO 3 ? reduction to NH 4 + (DNRA) or immobilization of NO 3 ? ). Total NO 3 ? consumption and the proportion of total NO 3 ? consumption to total NO 3 ? production decreased with an increase in the drought index of ecosystems, showing that strong interactions appear to exist between climatic condition (e.g. the drought index), N mineralization and the rate of DNRA. Interactions between vegetation, climatic conditions govern internal N cycling in these forests soils.  相似文献   

3.

Aims

To assess the effects of atmospheric N deposition on the C budget of an alpine meadow ecosystem on the Qinghai–Tibetan Plateau, it is necessary to explore the responses of soil-atmosphere carbon dioxide (CO2) exchange to N addition.

Methods

Based on a multi-form, low-level N addition experiment, soil CO2 effluxes were monitored weekly using the static chamber and gas chromatograph technique. Soil variables and aboveground biomass were measured monthly to examine the key driving factors of soil CO2 efflux.

Results

The results showed that low-level N input tended to decrease soil moisture, whereas medium-level N input maintained soil moisture. Three-year N additions slightly increased soil inorganic N pools, especially the soil NH 4 + -N pool. N applications significantly increased aboveground biomass and soil CO2 efflux; moreover, this effect was more significant from NH 4 + -N than from NO 3 ? -N fertilizer. In addition, the soil CO2 efflux was mainly driven by soil temperature, followed by aboveground biomass and NH 4 + -N pool.

Conclusions

These results suggest that chronic atmospheric N deposition will stimulate soil CO2 efflux in the alpine meadow on the Qinghai–Tibetan Plateau by increasing available N content and promoting plant growth.  相似文献   

4.
Soil microbial properties play a key role in belowground ecosystem functioning, but are not well understood in forest ecosystems under nitrogen (N) enrichment. In this study, soil samples from 0–10 cm and 10–20 cm layers were collected from a Dahurian larch (Larix gmelinii Rupr.) plantation in Northeast China after six consecutive years of N addition to examine changes in soil pH, nutrient concentrations, and microbial biomass and activities. Nitrogen addition significantly decreased soil pH and total phosphorus, but had little effect on soil total organic carbon (TOC) and total N (TN) concentrations. The NO 3 ? -N concentrations in the two soil layers under N addition were significantly higher than that in the control, while NH 4 + -N concentrations were not different. After six years of N addition, potential net N mineralization and nitrification rates were dramatically increased. Nitrogen addition decreased microbial biomass C (MBC) and N (MBN), and MBC/TOC and MBN/TN in the 0–10 cm soil layer, but MBC/MBN was increased by 67% in the 0–10 cm soil layer. Soil basal respiration, microbial metabolic quotient (qCO2), and β-glucosidase, urease, acid phosphomonoesterase and nitrate reductase activities in the two soil layers showed little change after six years of N addition. However, soil protease and dehydrogenase activities in the 0–10 cm layer were 41% and 54% lower in the N addition treatment than in the control, respectively. Collectively, our results suggest that in the mid-term N addition leads to a decline in soil quality in larch plantations, and that different soil enzymes show differentiated responses to N addition.  相似文献   

5.
Potassium nutrition of crops under varied regimes of nitrogen supply   总被引:9,自引:0,他引:9  
Biological soil crusts (BSCs) greatly influence the N cycle of semi-arid ecosystems, as some organisms forming them are able to fix atmospheric N. However, BSCs are not always taken into account when studying biotic controls on N cycling and transformations. Our main objective was to understand how BSCs modulate the availability of N in a semi-arid Mediterranean ecosystem dominated by the tussock grass Stipa tenacissima. We selected the six most frequent soil cover types in the study area: S. tenacissima tussocks (ST), Retama sphaerocarpa shrubs (RS), and open areas with very low (BS), low (LC) medium (MC) and high (HC) cover of well developed and lichen-dominated BSCs. The temporal dynamics of available N dynamics followed changes in soil moisture. Available NH 4 + -N did not differ between microsites, while available NO 3 - -N was substantially higher in the RS than in any other microsite. No significant differences in the amount of available NO 3 - -N were found between ST and BS microsites, but these microsites had more NO 3 - -N than those dominated by BSCs (LC, MC and HC). Our results suggest that BSCs may be inhibiting nitrification, and highlight the importance of this biotic community as a modulator of the availability of N in semi-arid ecosystems.  相似文献   

6.
To test the hypothesis that rhizosphere acidification would enhance the hydrolyzation of organic phosphates by increasing phosphatase activity. A Petri dish experiment with sterile agar and a pot experiment with a low P soil were used. In the Petri dish experiment, roots of each plant were cultured in two compartments, each of which contained agar with one of three nitrogen combinations: NH 4 + /N0 (N0 = nitrogen free), NH 4 + /NO 3 - , and NO 3 - /N0. Phytin was supplied as the sole phosphorus (P) source to all compartments. In the pot experiment, the soil in each pot was treated with N0, KNO3, or (NH4)2SO4) together with 0 or 75 mg kg?1 phytin-P. Dry weight, P concentration, and P content of roots were highest in the NH 4 + compartments in the Petri dish experiment. In the pot experiment, dry weight, P concentration, and P content of both shoots and roots were higher with NH 4 + than with NO 3 - . NH 4 + treatments reduced rhizosphere pH, promoted the hydrolization of phytin, enhanced acid phosphatase activity in the rhizosphere, and increased phytin-P utilization relative to N0 and NO 3 - treatments. Phosphatase activity was negatively correlated with rhizosphere pH but was positively correlated with plant P content in both experiments. Rhizosphere acidification optimized the activity of acid phosphatase excreted by maize roots and promoted phytin mineralization. NH 4 + -induced acidification in the maize rhizosphere improved the growth of maize roots by improving P uptake from phytin; the improved growth, in turn, increased NH 4 + uptake and acidification.  相似文献   

7.
Microbial N turnover processes were investigated in three different forest soil layers [organic (O) layer, 0–10 cm depth (M1), 10–40 cm depth (M2)] after the clear cutting of a nitrogen (N) saturated spruce stand at the Höglwald Forest (Bavaria, Germany). The aim of the study was to provide detailed insight into soil-layer specific microbial production and the consumption of inorganic N within the main rooting zone. Furthermore, we intended to clarify the relevance of each soil layer investigated in respect of the observed high spatial variation of seepage water nitrate (NO 3 ? ) concentration at a depth of 40 cm. The buried bag and the 15N pool dilution techniques were applied to determine the net and gross N turnover rates. In addition, soil pH, C:N ratio, pool sizes of soil ammonium (NH 4 + ) and NO 3 ? , as well as quantities of microbial biomass carbon (Cmic) and nitrogen (Nmic) were determined. The 40 cm thick upper mineral soil was found to be the main place of NO 3 ? production with a NO 3 ? supply or net nitrification three times higher than in the considerably thinner O layer. Nevertheless, O layer nitrification processes determined via in situ field experiments showed significant correlation with seepage water NO 3 ? . An improved correlation noted several months after the cut may result from a transport-induced time shift of NO 3 ? with downstream hydrological pathways. In contrast, the soil laboratory incubation experiments found no indication that mineral soil is relevant for the spatial heterogeneity of seepage water NO 3 ? . The results from our study imply that in situ experiments may be better suited to studies investigating N turnover in relation to NO 3 ? loss via seepage water in similar ecosystems in order to gain representative data.  相似文献   

8.
Utilization of nitrogen in the form of either nitrate (NO 3 ? ) or ammonium (NH 4 + ) ions may affect the carbohydrate metabolism and energy budget of plants. Recent studies showed that greater expenses of NO 3 ? to NH 4 + reduction mostly occur in the roots and during darkness. Fertilization of corn with 15N-labeled nitrate and ammonium, combined with pulse labeling of plants in a 14CO2 atmosphere at the V6 and V8 growth stages, allowed us to evaluate the effect of N form on the CO2 efflux from soil. NH 4 + oxidation was inhibited by adding dicyandiamide. In respect to ammonium, nitrate addition increased root-derived CO2 efflux from corn by 2.6 times at stage V6 and by 1.8 times at stage V8. The time of peak 14CO2 efflux from soil also differed between two growing stages: at V6, efflux peaked only on the second day after pulse labeling, while at V8 this occurred within the first 6 h. The strong effect of NO 3 ? and NH 4 + on root respiration requires considering the N form in the soil and the nitrate reduction site location in a plant when modeling soil respiration changes and when separately estimating individual CO2 sources that contribute to the total soil CO2 efflux.  相似文献   

9.
In semi-arid grassland ecosystems, soil biogeochemical processes are controlled by seasonal and inter-annual rainfall variation and temperature, which may override the long-term impact of grazers on N availability and N dynamics. In a three-year (2004?C2006) case study of an Inner Mongolian grassland, we analysed time-integrated (ion-exchange resins) and instantaneous (soil mineral N extractions) inorganic N availability at three sites of varying grazing intensities and combined these data with information on soil water content (SWC), aboveground net primary productivity (ANPP) and plant N uptake. Additionally, the effects of rainfall and grazing on N-form availability (NO 3 ? -N, NH 4 + -N) were considered. Grazing had less impact on N availability compared to seasonal and annual rainfall distribution. One of the three study years (2004) showed a grazing effect with higher resin-N availability at the ungrazed site compared to the heavily grazed site. Inorganic N availability was low in the driest year (2005) and highest in a year of average rainfall amount and favourable distribution (2004). In general, we found a positive relationship between inorganic N availability and both plant productivity and plant N uptake. Rainfall also controlled the plant available NO 3 ? -N and NH 4 + -N pools; NH 4 + -N dominated the available inorganic N-form in times of low SWC, while the available NO 3 ? -N increased with SWC. We observed N availability and plant productivity in a temporal synchronized pattern. Increased rainfall variability and land-use practices affecting SWC will likely alter N availability dynamics (and the relation of N-forms) and, therefore, important processes of semi-arid natural grassland carbon and N cycling.  相似文献   

10.

Background and aims

Climate warming, nitrogen (N) deposition and land use change are some of the drivers affecting ecosystem processes such as soil carbon (C) and N dynamics, yet the interactive effects of those drivers on ecosystem processes are poorly understood. This study aimed to understand mechanisms of interactive effects of temperature, form of N deposition and land use type on soil C and N mineralization.

Methods

We studied, in a laboratory incubation experiment, the effects of temperature (15 vs. 25 °C) and species of N deposition (NH4 +-N vs. NO3 ?-N) on soil CO2 efflux, dissolved organic C (DOC) and N (DON), NH4 +-N, and NO3 ?-N concentrations using intact soil columns collected from adjacent forest and grassland ecosystems in north-central Alberta.

Results

Temperature and land use type interacted to affect soil CO2 efflux, concentrations of DON, NH4 +-N and NO3 ?-N in most measurement times, with the higher incubation temperature resulted in the higher CO2 efflux and NH4 +-N concentrations in forest soils and higher DON and NO3 ?-N concentrations in grassland soils. Temperature and land use type affected the cumulative soil CO2 efflux, and DOC, DON, NH4 +-N and NO3 ?-N concentrations. The form of N added or its interaction with the other two factors did not affect any of the C and N cycling parameters.

Conclusions

Temperature and land use type were dominant factors affecting soil C loss, with the soil C in grassland soils more stable and resistant to temperature changes. The lack of short-term effects of the deposition of different N species on soil C and N mineralization suggest that maybe there was a threshold for the N effect to kick in and long-term experiments should be conducted to further elucidate the species of N deposition effects on soil C and N cycling in the studied systems.  相似文献   

11.
The present study reported the growth and metabolism characteristics of anaerobic ammonium-oxidizing (anammox) bacteria aggregates in an expanded granular sludge bed (EGSB). The results showed that the anammox bacteria aggregates presented starvation, growth, and inhibition phase along with the increase of substrate supply. The substrate conversion rates for survival were 0.05 kgNH 4 + –N/(kgVSS·day), 0.07 kgNO 2 ? –N/(kgVSS·day), and 0.12 kgN/(kgVSS·day); the substrate conversion rates for maximum growth were 0.21 kgNH 4 + –N/(kgVSS·day), 0.24 kgNH 4 + –N/(kgVSS·day), and 0.45 kgNH 4 + –N/(kgVSS·day), respectively. In the growth phase, the yield of anammox bacteria aggregates was 0.14 gVSS/(gNH 4 + –N), 0.12 gVSS/(gNO 2 ? –N), and 0.70 gVSS/(gNO 3 ? –N); the yield of extracellular polymeric substances (EPS) was 0.11 gEPS/(gNH 4 + –N), 0.09 gEPS/(gNO 2 ? –N), and 0.55 gEPS/(gNO 3 ? –N), respectively. The EPS contents in anammox bacteria aggregates were high compared to that in anaerobic granular sludge. Speculated from the cell yield, the energy for anammox bacteria growth was not only from nitrite oxidation, but also from anammox reaction.  相似文献   

12.
The effects of wetting and drying and inorganic nitrogen (N) addition on carbon (C) and N turnover in fresh Douglas fir litter (Speuld forest, the Netherlands) were investigated. Litter was incubated for 9 days in the laboratory, receiving different moisture and N addition treatments. Following the additions, a series of reactions were observed of which most notable were a rapid retention of added ammonium and nitrate (NO 3 - ) and a sudden increase in CO2 respiration. For the rewetted-and-moist incubations, respiration levels remained elevated, N was net immobilized and nitrous oxide (N2O) production increased throughout the experiment. About 80% of the NO 3 - produced was lost again as N2O. In the rewetted-and-dried incubations, respiration decreased during the drying phase; no clear patterns in N mineralization were detected; and N2O production remained at constant levels, but still resulted in gaseous loss for half of the NO 3 - net produced. The experiments thus revealed two important NO 3 - sinks in LF1 litter, namely rapid retention of added NO 3 - and gaseous loss as N2O. The maximum NO 3 - loss via these sinks was estimated at 2 kg-N ha-1 yr-1, which is small compared to annual NO 3 - leaching at 90 cm soil depth (31 kg-N ha-1 yr-1).  相似文献   

13.
The influence of NO 3 ? -N on growth and osmotic adjustment was studied in Tamarix laxa Willd., a halophyte with salt glands on its twigs. Seedlings of T. laxa Willd. were exposed to 1 mM (control) or 300 mM NaCl, with 0.05, 1 or 10 mM NO 3 ? -N for 24 days. The relative growth rate of seedlings at 300 mM NaCl was lower than that of control plants at all NO 3 ? -N levels, but the concentrations of organic N and total N in the twigs did not differ between the two NaCl treatments. Increasing NO 3 ? supply under 300 mM NaCl improved the growth of T. laxa, indicating that NO 3 ? played positive roles in improving salt resistance of the plant. The twigs of T. laxa Willd. accumulated mainly inorganic ions, especially Na+ and Cl?, to lower osmotic potential (Ψs): the contributions of Na+ and Cl? to Ψs were estimated at 31% and 27% respectively, at the highest levels of supply of both NaCl and NO 3 ? -N. The estimated contribution of NO 3 ? -N to Ψs was as high as 20% in the twigs in these conditions, indicating that NO 3 ? was also involved in osmotic adjustment in the twigs. Furthermore, increases in tissue NO 3 ? were accompanied by decreases in tissue Cl? and proline under 300 mM NaCl. The estimated contribution of proline to Ψs declined as with NO 3 ? -N supply increased from 1 to 10 mM, while the contributions of nitrate to Ψs were enhanced under 300 mM NaCl. This suggested that higher accumulation of nitrate in the vacuole alleviated the effects of salinity stress on the plant by balancing the osmotic potential. In conclusion, NO 3 ? -N played both nutritional and osmotic roles in T. laxa Willd. in saline conditions.  相似文献   

14.
The rapid expansion of intensively farmed vegetable fields has substantially contributed to the total N2O emissions from croplands in China. However, to date, the mechanisms underlying this phenomenon have not been completely understood. To quantify the contributions of autotrophic nitrification, heterotrophic nitrification, and denitrification to N2O production from the intensive vegetable fields and to identify the affecting factors, a 15N tracing experiment was conducted using five soil samples collected from adjacent fields used for rice-wheat rotation system (WF), or for consecutive vegetable cultivation (VF) for 0.5 (VF1), 6 (VF2), 8 (VF3), and 10 (VF4) years. Soil was incubated under 50% water holding capacity (WHC) at 25°C for 96 h after being labeled with 15NH4NO3 or NH 4 15 NO3. The average N2O emission rate was 24.2 ng N?kg?1 h?1 in WF soil, but it ranged from 69.6 to 507 ng N?kg?1 h?1 in VF soils. Autotrophic nitrification, heterotrophic nitrification and denitrification accounted for 0.3–31.4%, 25.4–54.4% and 22.5–57.7% of the N2O emissions, respectively. When vegetable soils were moderately acidified (pH, 6.2 to ?≥?5.7), the increased N2O emissions resulted from the increase of both the gross autotrophic and heterotrophic nitrification rates and the N2O product ratio of autotrophic nitrification. However, once severe acidification occurred (as in VF4, pH?≤?4.3) and salt stress increased, both autotrophic and heterotrophic nitrification rates were inhibited to levels similar to those of WF soil. The enhanced N2O product ratios of heterotrophic nitrification (4.84‰), autotrophic nitrification (0.93‰) and denitrification processes were the most important factors explaining high N2O emission in VF4 soil. Data from this study showed that various soil conditions (e.g., soil salinity and concentration of NO 3 - or NH 4 + ) could also significantly affect the sources and rates of N2O emission.  相似文献   

15.
A bacterial culture of Nitrosomonas sp. was isolated from a nitrifying biofilm to construct a biosensor for ammonium nitrogen (NH 4 + ?N) measurements in high ammonia wastewaters. The pure culture of microorganisms was immobilized into agarose gel matrix to attain a stable biosensor with a long service life. Biosensors were calibrated using (NH4)2SO4 solution and a steady-state method. Subsequently, several experiments with synthetic and industrial wastewaters were conducted. A linear range up to 20 mg/L of NH 4 + ?N, and sensitivities between 0.030 and 0.036 were gained with biosensors. During 14 days of stable service life of the Nitrosomonas sp. biosensors, variation of the signal was less than 7%. Response times of biosensors were 15 ~ 25 min, while recovery times were up to 25 min. Measurements with high ammonia content synthetic and industrial wastewaters were conducted, and 8.3 and 5.6% over estimation of NH 4 + ?N was gained, respectively, compared with results of Nessler method. In spite of the small overestimation, the biosensor based on a pure culture of Nitrosomonas sp. and calibrated with (NH4)2SO4 is suitable for the analysis of NH 4 + ?N in high ammonia content wastewaters.  相似文献   

16.
A discrepancy between plant demand and soil supply of nitrogen (N) has been observed in early successional stages of riparian vegetation in interior Alaska. We hypothesized that a hydrologically mediated N supply serves as a mechanism to balance this apparent deficiency of plant N supply. To test this hypothesis, we conducted a tracer experiment and measured the activity of nitrate reductase (NRA) over the summer on the early successional floodplain of the Tanana River in interior Alaska. Isotopic data showed that river-/groundwater was an important source of plant water and that hyporheic N could be absorbed by early successional species. Plant NRA generally increased as the growing season progressed, and NO 3 ? -N availability increased. Both Salix interior Rowlee and Populus balsamifera L. used NO 3 ? -N, and the timing of plant NRA relative to river discharge chemistry and soil NO 3 ? -N concentrations, strongly suggest that plant uptake of NO 3 ? -N is coupled to fluvial dynamics. Moreover, this physiological function helps explain the apparent discrepancy between N mineralization and productivity in these riparian ecosystems, and demonstrates that plant N availability in these riparian stands is under significant hydrological control.  相似文献   

17.
Labeled nitrogen (15?N) was applied to a soil-based substrate in order to study the uptake of N by Glomus intraradices extraradical mycelium (ERM) from different mineral N (NO 3 ? vs. NH 4 + ) sources and the subsequent transfer to cowpea plants. Fungal compartments (FCs) were placed within the plant growth substrate to simulate soil patches containing root-inaccessible, but mycorrhiza-accessible, N. The fungus was able to take up both N-forms, NO 3 ? and NH 4 + . However, the amount of N transferred from the FC to the plant was higher when NO 3 ? was applied to the FC. In contrast, analysis of ERM harvested from the FC showed a higher 15?N enrichment when the FC was supplied with 15NH 4 + compared with 15NO 3 ? . The 15?N shoot/root ratio of plants supplied with 15NO 3 ? was much higher than that of plants supplied with 15NH 4 + , indicative of a faster transfer of 15NO 3 ? from the root to the shoot and a higher accumulation of 15NH 4 + in the root and/or intraradical mycelium. It is concluded that hyphae of the arbuscular mycorrhizal fungus may absorb NH 4 + preferentially over NO 3 ? but that export of N from the hyphae to the root and shoot may be greater following NO 3 ? uptake. The need for NH 4 + to be assimilated into organically bound N prior to transport into the plant is discussed.  相似文献   

18.
It has been pointed out that tea (Camellia sinensis (L.) O. Kuntze) prefers ammonium (NH 4 + ) over nitrate (NO 3 ? ) as an inorganic nitrogen (N) source. 15N studies were conducted using hydroponically grown tea plants to clarify the characteristics of uptake and assimilation of NH 4 + and NO 3 ? by tea roots. The total 15N was detected, and kinetic parameters were calculated after feeding 15NH 4 + or 15NO 3 ? to tea plants. The process of N assimilation was studied by monitoring the dynamic 15N abundance in the free amino acids of tea plant roots by GC-MS. Tea plants supplied with 15NH 4 + absorbed significantly more 15N than those supplied with 15NO 3 ? . The kinetics of 15NH 4 + and 15NO 3 ? influx into tea plants followed a classic biphasic pattern, demonstrating the action of a high affinity transport system (HATS) and a low affinity transport system (LATS). The V max value for NH 4 + uptake was 54.5 nmol/(g dry wt min), which was higher than that observed for NO 3 ? (39.3 nmol/(g dry wt min)). KM estimates were approximately 0.06 mM for NH 4 + and 0.16 mM for NO 3 ? , indicating a higher rate of NH 4 + absorption by tea plant roots. Tea plants fed with 15NH 4 + accumulated larger amounts of assimilated N, especially glutamine (Gln), compared with those fed with 15NO 3 ? . Gln, Glu, theanine (Thea), Ser, and Asp were the main free amino acids that were labeled with 15N under both conditions. The rate of N assimilation into Thea in the roots of NO 3 ? -supplied tea plants was quicker than in NH 4 + -supplied tea plants. NO 3 ? uptake by roots, rather than reduction or transport within the plant, seems to be the main factor limiting the growth of tea plants supplied with NO 3 ? as the sole N source. The NH 4 + absorbed by tea plants directly, as well as that produced by NO 3 ? reduction, was assimilated through the glutamine synthetase-glutamine oxoglutarate aminotransferase pathway in tea plant roots. The 15N labeling experiments showed that there was no direct relationship between the Thea synthesis and the preference of tea plants for NH 4 + .  相似文献   

19.

Background and aims

Changes in soil moisture availability seasonally and as a result of climatic variability would influence soil nitrogen (N) cycling in different land use systems. This study aimed to understand mechanisms of soil moisture availability on gross N transformation rates.

Methods

A laboratory incubation experiment was conducted to evaluate the effects of soil moisture content (65 vs. 100% water holding capacity, WHC) on gross N transformation rates using the 15N tracing technique (calculated by the numerical model FLUAZ) in adjacent grassland and forest soils in central Alberta, Canada.

Results

Gross N mineralization and gross NH 4 + immobilization rates were not influenced by soil moisture content for both soils. Gross nitrification rates were greater at 100 than at 65% WHC only in the forest soil. Denitrification rates during the 9 days of incubation were 2.47 and 4.91 mg N kg-1 soil d-1 in the grassland and forest soils, respectively, at 100% WHC, but were not different from zero at 65% WHC. In the forest soil, both the ratio of gross nitrification to gross NH 4 + immobilization rates (N/IA) and cumulative N2O emission were lower in the 65 than in the 100% WHC treatment, while in the grassland soil, the N/IA ratio was similar between the two soil moisture content treatments but cumulative N2O emission was lower at 65% WHC.

Conclusions

The effect of soil moisture content on gross nitrification rates differ between forest and grassland soils and decreasing soil moisture content from 100 to 65% WHC reduced N2O emissions in both soils.  相似文献   

20.
Nitrogen (N) is a crucial nutrient for soil biota, and its cycling is determined by the organic carbon decomposing process. Some endophytic fungi are latent saprotrophs that trigger their saprotrophic metabolism to promote litter organic matter cycling as soon as the host tissue senesces or dies. However, the effects of endophytic fungi on litter and soil N dynamics in vitro have rarely been investigated. In this study, we investigated N dynamics (total and mineral N) in both litter and soil in incubations of a pure culture of an endophytic fungus Phomopsis liquidambari with litter and following soil burial of the litter. Soil enzymes and microbial communities participating in the N transformations were also investigated. A pure culture of P. liquidambari released litter NH 4 + –N in the initial stages (10 days) of the incubation. However, following soil burial, the presence of both P. liquidambari and soil ammonia-oxidizing bacteria (AOB) resulted in an increase in soil NO 3 ? –N. These results indicate that the endophytic fungus P. liquidambari in vitro stimulates organic mineralization and promote NH 4 + –N release. Such effects triggered soil AOB-driven nitrification process.  相似文献   

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