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《BMJ (Clinical research ed.)》1935,1(3862):69-70
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The potential for interspecific genetic exchange was examined by monitoring flowering patterns, pollinator movement, and gene flow among experimental populations of the Texas gourd (Cucurbita texana) and cultivars of Cucurbita pepo. While flowering patterns and pollinator movement tended to maximize self-pollination and local gene exchange, movement of effective pollen exceeded 1,300 m. This movement, mediated by the solitary bee Xenoglossa strenua and monitored by tracking allozyme variants, produced interspecific hybrids in 5% of the progeny from experimental plants. Interspecific gene exchange occurred in either direction with either species serving as staminate or pistillate parent. No obvious constraints to gene flow among plants representing C. texana and distinctive cultivars (vars. ovifera, medullosa, melopepo) of C. pepo were detected. Genetic exchange among different species and cultivars is enhanced by the foraging behavior of Xenoglossa. Multiple visits to either staminate (pollen carryover) or pistillate (multiple pollinations) flowers often result in the deposition of mixed pollen on receptive stigmas. The wild type (C. texana) can donate and receive effective pollen when growing under both weedy and natural conditions. The observed lack of interspecific reproductive isolation supports treatment of cultivars and wild types as a single species and, in conjunction with available data concerning temporal/geographical relationships among bees, squash, gourds, and humans in eastern North America, suggests the possibility of long-term genetic interaction between wild types and domesticates. 相似文献
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Cloning by synteny: identifying C. briggsae homologues of C. elegans genes. 总被引:4,自引:0,他引:4
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Phylogenetic comparisons of gene and protein sequences between related species are often used to identify evolutionarily conserved elements that are important for gene expression, function, or regulation. However, homologoues may sometimes be difficult to identify by conventional low stringency hybridisation techniques, if they have undergone substantial sequence divergence. A new approach, cloning by synteny, is described that was used to identify the C. briggsae homologue of the C. elegans sex-determining gene tra-2. We show that four genes tra-2, ppp-1, art-1, and sod-1 are organised in a syntenic cluster and suggest that extensive conservation of gene linkage may exist between C. briggsae and C. elegans. We have also constructed a C. briggsae cDNA library to facilitate characterisation of these genes. Given the rapid progress in the physical mapping and sequencing of the C. elegans genome, cloning by synteny may provide the fastest method for identifying C. briggsae gene homologues, especially for genes encoding novel proteins. 相似文献
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