相反相成药物治疗马骡结症的理论探讨

“对立统一规律是宇宙的根本规律。”动物体是完整统一的有机体,其各系统、各器官的机能活动是矛盾的,对立的,同时又是统一的。心脏的跳动,肺脏的呼吸,肌肉的伸缩,胃肠的蠕动等,都是由相互联系、相互制约的对立统一的活动完成的。在这些活动当中,神经系统起着主要的调节作用。中枢神经系统是支配全身活动的中枢,而内脏器官的活动是由相对自主的植     

云南一些植物特征成分与古夷平面的相关性

生理生态学的原则是随着纬度的升高,物种分布的海拔高度将随之降低。但是,云南一些植物特征成分的分布却与之相反,它们的分布与云南残留的古夷平面的分布相似,即随着纬度的升高,其分布的海拔高度也随之升高。二者的区别是云南古夷平面分布的趋势线的仰角大于那些特征成分分布的趋势线的仰角。可能的原因是：相对于剧烈的地质运动,物种的适应能力总是相对滞后的,并且物种的分布也受到其生长特性的制约;根据分布区形成的原则,这些成分的发生应早于夷平面的隆起。     

学案导学在高中生物教学中的应用

制作学案的过程,就是老师理解通透所传授的知识的前提下,阅读大量的相关的资料,根据学生的认知水平与知识的积累能力,为指导学生进行主动的知识建构而编制的学习方案,以便积极引导和帮助学生的自主学习的能力进行探究思索的方案。高中生物的教学研究问题,通过学案的方式向学生传授知识,是教师在教学理论与学习标准的指导下对高中生物的教学所提高的一个新层次。     

植物保护与农业可持续发展关系探究

随着社会的发展经济的进步我国在各个领域的发展上都得到了相应的提升,从农业的发展情况来看,由于我国在科学技术上进步故此在农业的发展上也取得了重大的成效,但是在农业发展的过程中也出现了一些严重的问题,就是对于化学药品的使用所造成的农产品的污染以及环境的污染。本文主要针对当前的一些植物保护的现状进行阐述,并对植物保护和农业的可持续发展间的关系进行了详细的分析探究,希望能够在此领域的学术发展起到一定的参考作用。     

自然和热凝固的人肝组织对KTP/YAG激光的光学特性的比较研究

本文采用双积分球测量系统和Inverse Add ing-Doub ling方法,研究了自然和热凝固的人肝组织对532nm的KTP激光和1 064 nm的Nd:YAG激光的光学特性。结果表明:热凝固的人肝组织对532 nm的KTP激光的吸收系数较自然的肝组织的吸收系数增大了23.5%(P<0.05),热凝固的肝组织对1 064 nm的Nd:YAG激光的吸收系数较自然的肝组织的吸收系数减小了34.3%(P<0.05)。热凝固的人肝组织对532 nm的KTP激光的散射系数较自然的肝组织的散射系数增大了4.50倍(P<0.05),热凝固的肝组织对1 064 nm的Nd:YAG激光的散射系数较自然的肝组织的散射系数增大了6.41倍(P<0.05)。热凝固的人肝组织对532 nm的KTP激光的各向异性因子较自然的肝组织的各向异性因子减小了5.47%,热凝固的肝组织对1 064 nm的Nd:YAG激光的各向异性因子较自然的肝组织的各向异性因子减小了1.95%。     

浅谈果树病虫害综合防治技术研究

在我国的农业生产的过程中,对于果树的病虫害综合防治是农业生产效益的根本保障,我国是一个农业大国,尤其是在我国的北方,果树的栽培更是有着悠久的历史,在近些年中由于技术的进步果树的品种更新的速度也在不断的加快,但是随之而来的就是果树的病虫害的问题,它对于果树的影响非常的大,直接在经济上给果农带来严重的损失,为了能够让果农的经济效益得到保障,对于果树的病虫害防治就显得极为重要。本文主要是对东北地区的果树病虫害的现状进行简要的分析,并对果树病虫害的综合防治技术进行研究找出适当的对策,希望能有所裨益。     

浅析如何做好基层林业站的工作

在当前的形势下,我国的林业建设工作也是需要及时的调整发展方案,以此来适应新时代的发展的,要由传统的林业建设模式逐渐的向现代化的方向发展。从某种程度上来说,基层林业站的微观变化也会对林业建设的发展方向的宏观调整产生直接的影响,因此,要结合科学发展的建设理念,来指导林业站的工作,以此来促进林业的健康建设。文章主要阐述基层林业站的作用,主要是为更好的发挥基层林业站的效用服务的。     

植物生理学研究与四化建设

一、植物生理学面临的挑战作为植物学的一个重要分支的植物生理学经过百余年的发展对植物生命活动的基本规律及其与环境的关系的了解取得了长足的进展,也对人类的生产活动起了巨大的促进作用。例如化学肥料及生长调节剂与除草剂的大规模应用,细胞全能性的发现及利用等。但与现代自然科学体系日新月异的进步相比植物生理学则象百舸竞渡中的一叶扁舟,面临着严峻的挑战。近年分子生物学和遗传学的飞速进步,各种物理化学检测手段的应用及检测精度的提高,使植物生理学的机理的探索必须建立在分子生物学的基础上并相应地使用精确的检测手段,例如植物激素乙烯的发现及其生物合成与作用机理的研究,就是建     

探讨中日环境管理存在的差异以及解决对策

环境状况的好与坏通常决定着一个国家的综合国力的强与弱。近几年来,随着社会的发展,环境方面的问题越来越多,各个国家对于环境问题的解决方法以及管理方式各有千秋,同时各个国家对于环境问题的管理也都十分的重视。而其中,日本环境的管理是开始于20世纪中期的,从20世纪中期到现在,短短的这一段时间中,日本的环境管理出现出人意料的成功,不仅创造了防治产业污染的奇迹,同时也形成了一套相对完成的管理体制以及丰富多彩的管理模式。与日本不同的是,我国的环境管理方面尚且存在一定的问题。本文将结合中日环境管理方面存在的差异探讨中国的环境管理存在的问题的解决对策。     

正常微生物群是一个新的人体生理学系统

1　前言微生态学的发展 ,现在已把微生物的作用 ,从主要是致病作用的观点转移到主要是生理作用的观点。这种观点的转变是经过 10 0多年的学术研究和科学发展才取得的。在 2 0世纪经过无菌动物的饲养、厌氧培养技术的进步和现代分子生物学、基因工程学的研究终于完成了微生态学向新的更高阶段的转变。这就是微生物的研究以致病性为主的时代转向以生理性为主的时代。要确定一门新学科的诞生日期是很难的。一门新学科的定义也是逐渐形成的 ,要经过科研成果不断积累才能逐渐完善。正常微生物群的生理作用的提出是很早的 ,至少要推衍到 19世纪的…     

A new method for spectrophotometric assay of activity of cross-linked penicillin acylase aggregates

A new method for monitoring reactions catalyzed by an immobilized enzyme, cross-linked penicillin acylase aggregates (PA CLEA), is suggested. Appropriate chromogenic substrates for spectrophotometric assay of catalytic activity of immobilized enzyme were chosen and their kinetic parameters determined. Active sites in PA CLEA preparations were titrated by the suggested method; it is shown that almost all active sites are retained during immobilization. This method is characterized as highly expressive, simple, and precise and may be used for control of PA immobilization efficiency as well as for study of operational, thermal, and pH stability of immobilized enzyme preparations.     

Protected immobilization of Taq DNA polymerase by active site masking on self-assembled monolayers of ω-functionalized thiols

A new efficient immobilization method that enables oriented immobilization of biologically active proteins was developed based on concepts of active site masking and kinetic control. Taq DNA polymerase was immobilized covalently on mixed self-assembled monolayers (SAMs) of ω-carboxylated thiol and ω-hydroxylated thiol through amide bonds between the protein and the carboxyl group in SAMs. Activity of the immobilized enzyme as large as 70% of solution-phase enzyme was achieved by masking the active site of the Taq DNA polymerase prior to the immobilization. In addition, the number of immobilization bonds was controlled by optimizing the carboxyl group concentration in the mixed monolayer. The maximum activity of immobilized Taq DNA polymerase was achieved at 5% of 12-mercaptododecanoic acid. The activity observed with protected immobilized enzyme was approximately 20 times higher than that observed with randomly immobilized enzyme. The maximum activity was acquired at a 1:1 DNA/enzyme masking ratio, immobilization pH 8.3, and within 10 min of reaction time. This concept of the active site masking and kinetic control of the number of covalent bonds between proteins and the surface can be generally applicable to a broad range of proteins to be immobilized on the solid surface with higher activity.     

Direct immobilization of tyrosinase enzyme from natural mushrooms (Agaricus bisporus) on D-sorbitol cinnamic ester

Mushroom tyrosinase was immobilized from an extract onto the totally cinnamoylated derivative of D-sorbitol by direct adsorption as a result of the intense hydrophobic interactions that took place. The immobilization pH value and mass of lyophilized mushrooms were important parameters that affected the immobilization efficiency, while the immobilization time and immobilization support concentration were not important in this respect. The extracted/immobilized enzyme could best be measured above pH 3.5 and the optimum measuring temperature was 55 degrees C. The apparent Michaelis constant using 4-tert-butylcatechol as substrate was 0.38+/-0.02 mM, which was lower than for the soluble enzyme from Sigma (1.41+/-0.20 mM). Immobilization stabilized the extracted enzyme against thermal inactivation and made it less susceptible to activity loss during storage. The operational stability was higher than in the case of the tyrosinase supplied by Sigma and immobilized on the same support. The results show that the use of p-nitrophenol as enzyme-inhibiting substrate during enzyme extraction and immobilization made the use of ascorbic acid unnecessary and is a suitable method for extracting and immobilizing the tyrosinase enzyme, providing good enzymatic activity and stability.     

双醛淀粉柔性固定木瓜蛋白酶研究

提出“柔性固定化酶”的模型,即：用一亲水、柔性高分子链接枝于载体表面制得柔性固定化载体,再用其以共价键合的方式进行酶的柔性固定化。其特点是：柔性固定可改善因直接固定化及手臂固定化使酶失活的缺陷,并提高固定化酶的自由度;如选用粒径单分散微球可改善固定化反应及固定化酶催化反应的均一性。以双醛淀粉(DAS)为柔性链对羧基化聚苯乙烯载体进行柔性化修饰后,固定木瓜蛋白酶,其活力回收率可达50％．相当于用戊二醛进行手臂固定化的活力回收率的2倍。     

Immobilization and properties of carminomycin 4-O-methyltranferase, the enzyme which catalyzes the final step in the biosynthesis of daunorubicin in Streptomyces peucetius

The carminomycin 4-O-methyltransferase enzyme from Streptomyces peucetius was covalently immobilized on 3M Emphaze ABI-activated beads. Optimal conditions of time, temperature, pH, ionic strength, enzyme, substrate (carminomycin), and cosubstrate (S-adenosyl-L-methionine) concentrations were defined for the immobilization reaction. Protein immobilization yield ranged from 52% to 60%. Including carminomycin during immobilization had a positive effect on the activity of the immobilized enzyme but a strongly negative effect on the coupling efficiency. The immobilized enzyme retained at least 57% of its maximum activity after storage at 4 degrees C for more than 4 months. The properties of the free and immobilized enzyme were compared to determine whether immobilization could alter enzyme activity. Both soluble and bound enzyme exhibited the same pH profile with an optimum near 8.0. Immobilization caused an approximately 50% decrease in the apparent K(m) (K'(m)) for carminomycin while the K'(m) for S-adenosyl-L-methionine was approximately doubled. A 57% decrease in the V(max) value occurred upon immobilization. These changes are discussed in terms of active site modifications as a consequence of the enzyme immobilization. This system has a potential use in bioreactors for improving the conversion of carminomycin to daunorubicin. (c) 1995 John Wiley & Sons, Inc.     

Immobilization and stabilization of a cyclodextrin glycosyltransferase by covalent attachment on highly activated glyoxyl-agarose supports

Covalent immobilization of cyclodextrin glycosyltransferase on glyoxyl-agarose beads promotes a very high stabilization of the enzyme against any distorting agent (temperature, pH, organic solvents). For example, the optimized immobilized preparation preserves 90% of initial activity when incubated for 22 h in 30% ethanol at pH 7 and 40 degrees C. Other immobilized preparations (obtained via other immobilization protocols) exhibit less than 10% of activity after incubation under similar conditions. Optimized glyoxyl-agarose immobilized preparation expressed a high percentage of catalytic activity (70%). Immobilization using any technique prevents enzyme inactivation by air bubbles during strong stirring of the enzyme. Stabilization of the enzyme immobilized on glyoxyl-agarose is higher when using the highest activation degree (75 micromol of glyoxyl per milliliter of support) as well as when performing long enzyme-support incubation times (4 h) at room temperature. Multipoint covalent immobilization seems to be responsible for this very high stabilization associated to the immobilization process on highly activated glyoxyl-agarose. The stabilization of the enzyme against the inactivation by ethanol seems to be interesting to improve cyclodextrin production: ethanol strongly inhibits the enzymatic degradation of cyclodextrin while hardly affecting the cyclodextrin production rate of the immobilized-stabilized preparation.     

聚乙烯醇-明胶复合膜固定化重组大肠杆菌NAD激酶的研究

为提高烟酰胺腺嘌呤二核苷酸(NAD)激酶的稳定性,采用复合膜对NAD激酶进行固定化研究。选用聚乙烯醇(PVA)、聚乳酸(PLA)、海藻酸钠(SA)和明胶(GEL)膜材料固定化NAD激酶。通过单因素实验确定最佳固定化条件为:PVA∶GEL为4∶1,加酶量为0.6 mL,固定化时间为6h,固定化温度为35℃,此时酶活力回收率达到最高值84%。固定化酶酶学性质分析结果表明,与游离酶进行比较,固定化后NAD激酶的最适温度由50℃提高至55℃,最适pH由8.0降至7.0,NAD激酶的热稳定性和pH稳定性均得到显著提高,但固定化酶的亲和力降低。固定化NAD激酶重复利用6次后,酶活性依然可维持初始酶活性的75%以上,表明聚乙烯醇-明胶复合膜固定化酶具有良好的操作稳定性。     

Electrostatic immobilization of pectinase on negatively charged AOT-Fe3O4 nanoparticles

Enzyme immobilization on magnetic nanoparticles (MNPs) has been a field of intense studies in biotechnology during the past decade. The present study suggests MNPs negatively charged by docusate sodium salt (AOT) as a support for pectinase immobilization. AOT is a biocompatible anionic surfactant which can stabilize MNPs. Electrostatic adsorption can occur between enzyme with positive charge and oppositely charged surface of MNPs (ca. 100 nm). The effect of three factors, i.e. initial enzyme concentration, aqueous pH and AOT concentration in different levels was investigated on pectinase immobilization. Maximum specific activity (1.98 U/mg enzyme) of immobilized pectinase and maximum enzyme loading of 610.5 mg enzyme/g support was attained through the experiments. Initial enzyme concentration is significantly important on both loading and activity of immobilized enzyme, while pH and AOT concentration only affect the amount of immobilized enzyme. Immobilized enzyme on MNPs was recovered easily through magnetic separation. At near pH of immobilization, protein leakage in reusability of immobilized enzyme was low and activity loss was only 10–20% after six cycles. Since pH is associated with immobilization by electrostatic adsorption, the medium pH was changed to improve the release of protein from the support, as well. MNPs properties were investigated using Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FT-IR) spectroscopy, and Dynamic Light Scattering (DLS) analysis.     

Optimization of urease immobilization onto non-porous HEMA incorporated poly(EGDMA) microbeads and estimation of kinetic parameters.

Jack bean urease (urea aminohydrolase, EC 3.5.1.5) was immobilized onto modified non-porous poly(ethylene glycol dimethacrylate/2-hydroxy ethylene methacrylate), (poly(EGDMA/HEMA)), microbeads prepared by suspension copolymerization for the potential use in hemoperfusion columns, not previously reported. The conditions of immobilization; enzyme concentration, medium pH, substrate and ethylene diamine tetra acetic acid (EDTA) presence in the immobilization medium in different concentrations, enzyme loading ratio, processing time and immobilization temperature were investigated for highest apparent activity. Immobilized enzyme retained 73% of its original activity for 75 days of repeated use with a deactivation constant kd = 3.72 x 10(-3) day(-1). A canned non-linear regression program was used to estimate the intrinsic kinetic parameters of immobilized enzyme with a low value of observable Thiele modulus (phi < 0.3) and these parameters were compared with those of free urease. The best-fit kinetic parameters of a Michaelis-Menten model were estimated as Vm = 3.318 x 10(-4) micromol/s mg bound enzyme protein, Km = 15.94 mM for immobilized, and Vm = 1.074 micromol NH3/s mg enzyme protein, Km = 14.49 mM for free urease. The drastic decrease in Vm value was attributed to steric effects, conformational changes in enzyme structure or denaturation of the enzyme during immobilization. Nevertheless, the change in Km value was insignificant for the unchanged affinity of the substrate with immobilization. For higher immobilized urease activity, smaller particle size and concentrated urease with higher specific activity could be used in the immobilization process.     

Efficient immobilization technique for enhancement of cellobiose dehydrogenase activity on silica gel

In this study, cellobiose dehydrogenase (CDH) of Phanerochaete chrysosporium ATCC 32629 was immobilized on silica gel for the further application of CDH in the saccharification process of biomass. To prevent the loss of enzyme activity during enzyme immobilization, the pretreatment of CDH was performed by various pretreatment materials before immobilization. When pretreated enzymes were used in immobilization, the activities of immobilized CDH were higher than non-pretreated CDH even in same amounts of immobilized protein. The specific activity of pretreated immobilized CDH with lactose was about two times higher than that of non-pretreated immobilized CDH. Moreover, the pretreated immobilized CDH showed better reusability than non-pretreated immobilized CDH, with 67.3% of its original activity being retained after 9 reuses.