湖南省蚋属绳蚋亚属二新种记述(双翅目,蚋科)

记述采自湖南省张家界国家森林公园的蚋属Simulium绳蚋亚属Gomphostilbia 2新种,即金鞭绳蚋S.(G.)jinbianense sp.nov,湖南绳蚋S.(G.)hunaiense sp.nov,二者分别以其产地命名.前者主要鉴别特征是两性胫节均具亚基黑环,而后者的雄性后足基跗节呈纺锤形.除对不同虫期作了形态描述外还提供了有关特征图.     

Two new species of Simulium (Gomphostilbia) (Diptera, Simuliidae) from Peninsular Malaysia, with keys to Peninsular Malaysian members of the Simulium ceylonicum species-group

Two new species of black flies, Simulium (Gomphostilbia) roslihashimisp. n. and Simulium (Gomphostilbia) lurauensesp. n., are described on the basis of reared adult, pupal and larval specimens collected from Peninsular Malaysia. These two new species are placed in the ceylonicum species-group within the subgenus Gomphostilbia. Simulium (Gomphostilbia) roslihashimisp. n. is most distinctive with the male having almost entirely yellow antennae, and Simulium (Gomphostilbia) lurauensesp. n. is characterized in the female by having the elongate sensory vesicle and the yellowish-white hairs on the base of the costal vein and on the stem vein, in the male by the greater number of large upper-eye facets and the spindle-shaped hind basitarsi which are much narrower than the hind tibiae and femora and in the pupa by the small terminal hooks. Keys to species of the ceylonicum species-group reported from Peninsular Malaysia are provided for females, males, pupae and mature larvae.     

Contribution to the knowledge on black flies (Diptera: Simuliidae) as vectors of Leucocytozoon (Haemosporida) parasites in Lithuania

Black flies (Diptera: Simuliidae) are among the most bothersome blood-sucking dipterans causing severe irritation and distress to poultry, wild birds, animals, and humans globally. These insects are vectors of viruses, bacteria, parasitic protozoans, and nematodes of humans and animals. Parasitic protozoa belonging to Haemosporida (Apicomplexa) are distributed worldwide and black flies are the principal vectors of avian haemosporidian parasites of the genus Leucocytozoon, a common parasite of birds. Based on the detection of parasite DNA in insects, 13 black fly species were reported to be potential vectors of Leucocytozoon in Europe. Information about which species of Simulium can play a role in the transmission of Leucocytozoon parasites is insufficient and needs to be developed. The aim of our study was to determine which black fly species are involved in the transmission of Leucocytozoon parasites in the Eastern Europe. The black fly females were collected in Lithuania using entomological net. They were morphologically identified, dissected to prepare salivary glands preparations, and then screened for the presence of Leucocytozoon parasites using microscopy and PCR-based methods. In all, we collected 437 black fly females belonging to eight species. The DNA of Leucocytozoon (genetic lineage lCOCO18) was detected in one of analysed females identified as Simulium maculatum. All salivary gland preparations were negative for the presence of Leucocytozoon sporozoites. Our results included S. maculatum as a potential vector of Leucocytozoon parasites. Increasing the knowledge on vector ecology, behaviour and improving collection methods may be the key to understand the evolution and diversity of these parasites.     

陕西省蚋属一新种记述(双翅目:蚋科)

Simulium (Simulium) liubaense, sp. nov. is described based on the female specimens collected from Liuba, Shaanxi Province, China. This species is assigned to the variegotum-group of the subgenus Simulium, and is closely related to S. (S.) jingfui Cai and An, 2008, S. (S.) taiwanicum Takaoka, 1979, S. (S.) hackeri Edwards 1928, S. (S.) chamlongi Takaoka and Suzuki, 1984. However it is clearly differentiated from them by the structure of cibarium, genital fork, genital plate, paraproct and cercus of the female. All the specimens are kept in the Medical Entomology Collection Gallery, Academy of Military Medical Sciences, Beijing.     

中国西双版纳蚋类纪要及一新种(双翅目,蚋科)

记载西双版纳自然保护区蚋类16种,隶属于蚋属Simulium的3亚属,其中包括1个中国新纪录种和3个待定种,并记述1新种,版纳绳蚋S．(G．)bannaense sp．nov．。该新种蛹具10条呼吸丝,与其已知4个近缘种即重庆绳蚋S．(G)chongqingense以及产白爪哇的S．(G．)batoense、产自印尼的S．(G．)atratoides和产自菲律宾的S．(G．)bi-colense等在形态学上有明显的种间差异。     

海南岛蚋类研究Ⅰ绳蚋三新种记述(双翅目:蚋科)

记述采自海南岛尖峰岭和五指山的蚋属绳蚋亚属Simulium(Gomphostilbia)3新种,即曲端绳蚋S.(G.)curvastylum sp.nov.,黑股绳蚋S.(G.)nigrofemoralum sp.nov.和膨股绳蚋S.(G.)tumum sp.nov..分别对其成虫和幼期作形态描述并与其近缘种比较作分类讨论.     

广东蚋属二新种记述 (双翅目:蚋科)

广东蚋属二新种记述（双翅目：蚋科）安继尧郝宝善＊严格（军事医学科学院微生物流行病研究所北京１０００７１）＊广州军区防疫队１９９５－０５－０５收稿,１９９６－０８－２２收修改稿１９９０年４月,在广东省上川岛采蚋时,发现黄足真蚋Ｓｉｍｕｌｉｕｍ（Ｅｕｓｉ...     

The phylogeny of Simulium (Chirostilbia) (Diptera: Simuliidae) and perspectives on the systematics of the genus in the Neotropical Region

Recently, knowledge of Neotropical Simuliidae has been accumulating quickly. However, information about supra-specific relationships is scarce and diagnoses of Simulium subgenera are unsatisfactory. To investigate the relationships among Simulium (Chirostilbia) species and test the subgenus monophyly, we performed a cladistic analysis. The ingroup included all species of this subgenus and the outgroup included representatives of the 17 species groups of Neotropical Simulium and three Holarctic species. The study was based on a data matrix with 31 terminal taxa and 45 morphological characteristics of adult, pupa and larva. The phylogenetic analysis under equal weights resulted in eight most-parsimonious trees (length = 178, consistency index = 34, retention index = 67). The monophyly of the S. (Chirostilbia) was not supported in our analysis. The Simulium subpallidum species group was closer to Simulium (Psilopelmia) and Simulium (Ectemnaspis) than to the Simulium pertinax species group. Additionally, we describe the three-dimensional shape of the terminalia of male and female of Simulium (Chirostilbia) for the first time and provide comments about the taxonomic problems involving some species of the subgenus: Simulium acarayense, Simulium papaveroi, S. pertinax, Simulium serranum, Simulium striginotum and S. subpallidum.     

梵净山绳蚋二新种 (双翅目 :蚋科)(英文)

本文首次报道贵州省梵净山自然保护区绳蚋二新种 ,即梵净山绳蚋Simulium (Gomphostilbia) fan jingshanesesp .nov .和孟氏绳蚋S .(G .)mengisp .nov .对其两性成虫、蛹和幼虫进行全面描述并分别与其近缘种进行分类讨论。     

Cryptic biodiversity and phylogenetic relationships revealed by DNA barcoding of Oriental black flies in the subgenus Gomphostilbia (Diptera: Simuliidae)

Understanding the medical, economic, and ecological importance of black flies relies on correct identification of species. However, traditional taxonomy of black flies is impeded by a high degree of morphological uniformity, especially the presence of cryptic biodiversity, historically recognized by details of chromosomal banding patterns. We assess the utility of DNA barcoding, based on cytochrome c oxidase subunit 1 (COI) sequences, for identifying 13 species of Oriental black flies in the subgenus Gomphostilbia. Samples of larvae fixed in Carnoy's solution were used to gather molecular and chromosomal data from the same individual. We found that larvae refrigerated in Carnoy's fixative for as long as 11 years can be used for DNA study. Levels of intraspecific genetic divergence, based on the Kimura-2 parameter, range from 0% to 9.28%, with a mean of 2.75%, whereas interspecific genetic divergence ranges from 0.34% to 16.05%. Values of intraspecific and interspecific genetic divergence overlap in seven species owing to incomplete lineage sorting and imperfect taxonomy, implying that DNA barcoding to identify these species will be ambiguous. Despite a low level of success, we found that DNA barcoding is useful in revealing cryptic biodiversity, potentially facilitating traditional taxonomy. Phylogenetic analyses indicate that species groups currently recognized on morphological criteria are not monophyletic, suggesting a need to reevaluate the classification of the subgenus Gomphostilbia.     

广西绳蚋二新种(双翅目,蚋科)

记述广西壮族自治区蚋属绳蚋亚属2新种,元宝山绳蚋S.(G.)yuanbaoshanense sp.nov.和龙胜绳蚋S.(G.)longshengense sp.nov.,二者均以采集地命名.模式标本存放于贵阳医学院生物学教研室.元宝山绳蚋,新种S.( G.)yuanbaoshanense sp.nov.(图1～14)新种雄虫后足基跗节不膨胀,两侧平行,隶属于巴托绳蚋组batoense group.与报道自爪哇的巴托绳蚋S.(G.)batoense Edwards和产自我国的金鞭绳蚋S.(G.)jinbianense Zhang and Chen近缘.但新种依其雄虫尾器的形状、蛹呼吸丝的分支情况及幼虫后颊裂端圆等特征与前者有明显的差异.而股、胫节无鳞状毛、胫节无亚基黑环及雌虫生殖叉突后臂无侧突,可与后者相区别.正模♀,副模2(♂)(♂),7蛹,3幼虫,均采自广西元宝山溪流水草中.龙胜绳蚋,新种S.(G.)longshengense sp.nov.(图15～27)根据新种雄虫后基跗节不膨胀,也隶属于绳蚋亚属的batoense组(Takaoka and Davies,1995,1996).与报道自中国的海南绳蚋S.(G.)hainananse Long and An近似.二者蛹的特征几乎无区别,但新种雄虫生殖肢端节和腹板的形状,各足股节具暗色鳞状毛;幼虫后颊裂端圆,腹节无暗色分支毛,可资鉴别.正模♀,副模3蛹,1幼虫采自广西龙胜县三门山溪背阳水草中;3蛹,1幼虫采自广西十万大山小溪水草中.     

广东蚋属二新种记述(双翅目：蚋科)

Five speices of Simuliidae, Simulium (Eusimulium) aureohirtum Brunetti,1911, S. (Simulium ) quinquestriatum (Shiraki, 1935) S.(Gomphostilbia ) pingxiangense An & Hao, 1990, S.(S.) shangchuanense sp. Nov. , S.(S.) spoonatum sp. Nov. were collected from Shangchuan Island, Guangdong Province, China. Both adult and pupal stages of the new species are described in this paper. All the specimens are kept in the Medical Entomological Specimen Building, Institute of Microbiology and Epidemiology Academy of Military Medical Sciences, Beijing.     

滋生于海南岛五指山瀑布岩壁上蚋属二新种(双翅目,蚋科)

首次记述我国大陆孳生于海南岛五指山瀑布岩壁上蚋属2新种,即瀑布蚋Simulium ( Simulium ) waterfallum sp. nov.和副瀑布蚋S. ( S. ) parawaterfallum sp. nov.二者均隶属于蚋亚属Simulium的多条蚋组multistriatum-group以其孳生环境命名.文中对其两性成虫、蛹和幼虫进行描述并分别与其近缘种进行分类讨论.模式标本存放于贵阳医学院生物学教研室.     

Comparison of mitochondrial DNA sequences of seven morphospecies of black flies (Diptera: Simuliidae)

Universal primers constructed from the 16S ribosomal RNA gene in the Drosophila yakuba mitochondrial genome were successfully used to amplify, via the polymerase chain reaction, the homologous region of mitochondrial DNA from seven black fly morphospecies. Amplification was achieved from single larval salivary glands and from single adults preserved in Carnoy's fixative (ethanol - acetic acid, 3:1), allowing DNA sequences and polytene chromosome banding pattern data to be gathered from the same individuals. Nucleotide sequences of the amplified DNA segment (347 base pairs) were obtained from all the species examined. As in Drosophila, the nucleotide base composition of the sequenced segment from black flies had a high adenine (A) and thymine (T) content (A + T on average comprised 77% of all nucleotides.). Nucleotide differences among the seven species were observed at 59 positions (55 nucleotide substitutions and 4 deletions). There were more transversion differences than transition differences both among and within genera; the proportion of transversions was higher between genera than within genera. Most transversion differences were A----T type, comprising 79% of all transversion differences and 50% of all sequence differences. Phylogenetic inference based strictly on transversion differences confirmed traditional generic and tribal groupings, i.e., Prosimulium fuscum (Syme & Davies) is close to Prosimulium magnum (Dyar & Shannon); Simulium decorum (Walker), Simulium venustum s.l. (Say), and Simulium vittatum s.l. (Zetterstedt) are close to each other; Stegopterna mutata (Malloch) and Cnephia dacotensis (Dyar & Shannon), which belong to the tribe Cnephiini, are grouped together.     

西藏蚋属一新种（双翅目：蚋科）

记述西藏自治区蚋属Simulium蚋亚属Simulium 1新种,以陈汉彬教授的名命名为汉彬蚋Simulium(Simulium) hanbini sp. nov.。该新种与Simulium (Simulium) jingfui Cai & An, 2008及Simulium (Simulium)liubaense Liu & An, 2009相似,但该蚋爪无基齿,拉氏器较长,食窦的形状也不同。模式标本保存在北京军事医学科学院医学昆虫标本馆。     

Geographical versus ecological isolation of closely related black flies (Diptera: Simuliidae) inferred from phylogeny, geography, and ecology

To investigate patterns of geographical and ecological separation among morphologically similar, closely related species of black flies, we integrated ecological, geographical, and phylogenetic information, based on multiple gene sequences, for 12 species in the subgenus Gomphostilbia in Thailand. Molecular characters supported the monophyly of the Simulium ceylonicum species group, but not of the Simulium batoense species group, suggesting that revisionary work is needed for the latter. Both ecological and geographical isolation of similar taxa were revealed. Stream velocity and altitude were among the principal ecological factors differing between closely related species. Most closely related species in the subgenus Gomphostilbia overlap geographically, suggesting the possibility of sympatric speciation driven by ecological divergence. Geographical isolation via dispersal also might have contributed to species divergence, while Pleistocene climate changes possibly influenced population genetic structure, demographic history, and speciation of some members of the subgenus.     

Salivary gland hypertrophy virus of house flies in Denmark: prevalence, host range, and comparison with a Florida isolate

House flies (Musca domestica) infected with Musca domestica salivary gland hypertrophy virus (MdSGHV) were found in fly populations collected from 12 out of 18 Danish livestock farms that were surveyed in 2007 and 2008. Infection rates ranged from 0.5% to 5% and averaged 1.2%. None of the stable flies (Stomoxys calcitrans), rat-tail maggot flies (Eristalis tenax) or yellow dung flies (Scathophaga stercoraria) collected from MdSGHV-positive farms displayed characteristic salivary gland hypertrophy (SGH). In laboratory transmission tests, SGH symptoms were not observed in stable flies, flesh flies (Sarcophaga bullata), black dump flies (Hydrotaea aenescens), or face flies (Musca autumnalis) that were injected with MdSGHV from Danish house flies. However, in two species (stable fly and black dump fly), virus injection resulted in suppression of ovarian development similar to that observed in infected house flies, and injection of house flies with homogenates prepared from the salivary glands or ovaries of these species resulted in MdSGHV infection of the challenged house flies. Mortality of virus-injected stable flies was the highest among the five species tested. Virulence of Danish and Florida isolates of MdSGHV was similar with three virus delivery protocols, as a liquid food bait (in sucrose, milk, or blood), sprayed onto the flies in a Potter spray tower, or by immersiion in a crude homogenate of infected house flies. The most effective delivery system was immersion in a homogenate of ten infected flies/ml of water, resulting in 56.2% and 49.6% infection of the house flies challenged with the Danish and Florida strains, respectively.     

青海特蚋亚属一新种(双翅目:蚋科)

Simulium (Tetisimulium) xiaodaoense sp. nov. is described based on the female specimens collected from Qinghai, China. This species is assigned to the subgenus Tetisimulium, and is closely related to S. (T.) tachengense An and Mahe, 1994 and S. (T.) wutaishanense An and Yan, 2003. However, it is clearly differentiated from them by the structure of gonapophyses, genital fork, genital plate, paraproct and cercus of the female. All the specimens are kept in the Medical Entomology Collection Gallery, Academy of Military Sciences, Beijing.     

Characterization and description of a virus causing salivary gland hyperplasia in the housefly,Musca domestica

Abstract. A double-stranded DNA virus was isolated from hyperplasic salivary glands of male and female houseflies, Musca domestica L. (Diptera: Muscidae), collected from a dairy in Alachua County, Florida, U.S.A. Sodium dodecyl sulphate (SDS)–polyacrylamide gel electrophoresis (PAGE) of this housefly salivary gland hyperplasia (SGH) virus revealed the presence of two major and eight minor structural polypeptides. Restriction endonuclease analysis indicated that the c. 137 kilobase pair DNA was double-stranded. Weekly sweep-net sampling of the fly population throughout the season (May-October, 1991) showed that 1.5-18.5% of the dissected flies possessed hyperplasic salivary glands. The virus replicated within the nuclei of the salivary gland cells and was transmitted per os to newly-emerged healthy adult flies.     

兴义维蚋多线染色体研究（英文）

首次在国内对兴义维蚋Simulium (Wilhelmia) xingyiense的多线染色体进行研究, 并提供其多线染色体标准图。选取兴义维蚋的成熟幼虫, 用改良苯酚品红染色法进行唾腺多线染色体制备, 并进行测量、 描述及分析。结果表明： 兴义维蚋多线染色体数目为3对（2n=6）。Ⅰ号染色体具中央着丝粒, Ⅱ和Ⅲ号染色体均为亚中央着丝粒染色体。核仁组织者区位于Ⅰ号染色体短臂近着丝粒端。巴尔比尼氏环和双泡位于Ⅱ号染色体短臂近中央位置。3对染色体的着丝粒区可形成明显的染色中心。兴义维蚋多线染色体具有多态性的倒位, 倒位频率为0.64。兴义维蚋多线染色体的着丝粒、 核仁组织区、 巴氏环、 双泡等主要特征性结构的位置及形态恒定一致,可作为该种的重要鉴别特征。其多态性的倒位可为该蚋种在细胞水平上进行蚋类分类鉴别和系统发育等研究提供基础资料。