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1.
Abstract B biotype is a reasonably important biotype among all known biotypes in the Bemisia tabaci species complex. Local populations of B. tabaci on different host plants were collected from across the Chinese mainland, Taiwan, Pakistan and Israel. From each population of B. tabaci , an internally transcribed spacer region of the ribosomal rDNA gene was amplified, cloned and the sequence determined. Sequence homology analyses were performed and the results were similar to those based on morphology and biological characters. Based on analysis of the internally transcribed spacer 1 sequences, a B biotype-specific primer was designed. The PCR diagnosis results showed that B biotype is identifiable by a specific PCR product by using the forward diagnostic primer paired with a universal reverse primer. This diagnostic primer-based protocol can be used for preliminary analysis of mixed Bemisia populations containing B biotype, as well as other biotypes.  相似文献   

2.
The two most widespread biotypes of Bemisia tabaci (Gennadius) in southern Europe and the Middle East are referred to as the B and Q-type, which are morphologically indistinguishable. In this study various DNA markers have been developed, applied and compared for studying genetic diversity and distribution of the two biotypes. For developing sequence characterized amplified regions (SCAR) and cleaved amplified polymorphic sequences (CAPS) techniques, single random amplified polymorphic DNA (RAPD) fragments of B and Q biotypes, respectively, were used. The CAPS were investigated on the basis of nuclear sodium channel and the mitochondrial cytochrome oxidase I genes (mtCOI) sequences. In general, complete agreement was found between the different markers used. Analysis of field samples collected in Israel for several years, using these markers, indicated that the percentage of the Q biotype tends to increase in field populations as time progresses. This may be attributed to the resistance of the Q biotype to neonicotinoids and pyriproxyfen and the susceptibility of the B biotype to these insecticides.  相似文献   

3.
The use of RAPD PCR to identify the B biotype of the whitefly Bemisia tabaci and distinguish it from other biotypes and species of whitefly is described. the technique enables the use of alcohol preserved material instead of live or frozen material as required by allozyme electrophoresis and demonstrates that eggs, juvenile stages and males or females can all be used.  相似文献   

4.
A field survey in western Sydney found populations of Bemisia tabaci biotype B (silverleaf whitefly) that have the potential to damage greenhouse vegetable crops prevalent in the region. Adult whiteflies were collected from five farms at 2-week intervals and the proportion of silverleaf whitefly in the overall whitefly population was determined using polyacrylamide gel electrophoresis. High levels of silverleaf whitefly were detected at most sampling dates on two of the farms surveyed and a third farm exhibited lower but relatively continuous silverleaf whitefly abundance. The presence of permanent silverleaf whitefly populations must be considered by the greenhouse industry when formulating whitefly management plans for western Sydney.  相似文献   

5.
烟粉虱B型和Q型群体遗传结构的RAPD分析   总被引:3,自引:2,他引:1  
近20年来,烟粉虱B型传入世界各地并暴发成灾,成为一种重要的农业入侵害虫; 烟粉虱Q型则是近几年引起人们高度重视的一种新的入侵生物型,目前已传入许多国家并造成一定危害。本文利用RAPD分子标记对烟粉虱B型和Q型不同地理种群的遗传结构进行了分析。结果表明:(1)引物H16对烟粉虱B型不同种群扩增的特异带,能有效区分烟粉虱B型和Q型、浙江非B/Q型种群;(2)烟粉虱Q型种群各项遗传多样性指数均比烟粉虱B型的要高;(3)我国烟粉虱Q型来自伊比利亚半岛的可能性比来自中东地区的可能性要大。另外,聚类分析结果提示,RAPD分子标记能有效地区分烟粉虱不同生物型,但可能不适用于生物型之间亲缘关系分析。  相似文献   

6.
The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is a worldwide pest and a vector of numerous plant viruses. B. tabaci is composed of dozens of morphologically indistinguishable biotypes and its taxonomic status is still controversial. This phloem-feeder harbours the primary symbiont Portiera aleyrodidarum and potentially six secondary symbionts: Cardinium, Arsenophonus, Hamiltonella, Rickettsia, Wolbachia and Fritschea. In the southwest Indian Ocean, La Réunion hosts two biotypes of this species: B (invasive) and Ms (indigenous). A multiplex PCR was developed to study the symbiont community of B. tabaci on La Réunion. Symbiont community prevalence and composition, host mitochondrial and nuclear genetic diversity, as well as host plant and localization, were described on field populations of La Réunion for B and Ms B. tabaci biotypes and their hybrids. A clear association between symbiotypes and biotypes was shown. Cardinium, Arsenophonus and Rickettsia were found in the Ms biotype (73.6%, 64.2% and 3.3%, respectively). Hamiltonella (exclusively) and Rickettsia were found in the B biotype (78% and 91.2%, respectively). Hybrids harboured all symbiotypes found in Ms and B populations, but with a higher prevalence of Ms symbiotypes than expected under random hybridization. An unexpected majority was Cardinium mono-infected (65.6%), and a striking minority (9%) harboured Cardinium/Arsenophonus. In the hybrids only, genetic diversity was linked to symbiotype. Among the hybrids, significant links were found between symbiotypes and: (i) mitochondrial COI sequences, i.e. maternal origin; and (ii) alleles of nuclear microsatellite loci, specific to either Ms or B parental biotype. Taken together, our results suggest that Cardinium and/or Arsenophonus may manipulate the reproduction of indigenous (Ms) with invasive (B) biotypes of Bemisia tabaci.  相似文献   

7.
The role of vector–begomovirus–plant interactions in the widespread invasion by some members of the whitefly species complex Bemisia tabaci is poorly understood. The invasive B biotype of B. tabaci entered China in the late 1990s and had become the predominant or only biotype of the whitefly in many regions of the country by 2005–2006. Meanwhile epidemics of begomoviruses have been observed in many crops including tomato for which Tomato yellow leaf curl China virus (TYLCCNV) and Tomato yellow leaf curl virus (TYLCV) have been identified as two major disease-causing agents. Here, we conducted laboratory experiments to compare the performance of the invasive B and indigenous ZHJ1 whitefly biotypes on uninfected, TYLCCNV-infected and TYLCV-infected plants of tomato cv. Hezuo903, a cultivar that has been widely cultivated in many regions of China. The infection of tomato plants by either of the viruses had no or only marginal effects on the development, survival and fecundity of the B biotype. In contrast, survival and fecundity of the ZHJ1 biotype were significantly reduced on virus-infected plants compared to those on uninfected plants. Populations of the B biotype on uninfected and TYLCCNV-infected plants increased at similar rates, whereas population increase of the ZHJ1 biotype on TYLCCNV-infected plants was affected adversely. These asymmetric responses to virus infection of tomato plants between the B and ZHJ1 biotypes are likely to offer advantages to the B biotype in its invasion and displacement of the indigenous biotype.  相似文献   

8.
The potential of populations of Bemisia tabaci (Genn.) to become resistant to insecticides has stimulated research into alternative tactics of integrated pest management such as the induction of host-plant resistance. Recent data have shown that silicon can increase the degree of resistance of host plants to insect pests. Therefore the aim of our work was to study the effects of silicon application on the vegetative development of soybean plants and on the induction of resistance to the silverleaf whitefly, B. tabaci biotype B. We performed choice and no-choice tests of oviposition preference on two soybean cultivars, IAC-19 (moderately resistant to B. tabaci biotype B) and MONSOY-8001 (susceptible), with and without application of silicon. Silicon did not affect silverleaf whitefly oviposition preferences, but caused significant mortality in nymphs. Thus, silicon increased the degree of resistance to silverleaf whitefly. Silicon decreased the production of phenolic compounds, but did not affect lignin production. However, when applied to cultivar IAC-19, it increased the production of non-protein organic nitrogen. Silicon had no effect on the vegetative development of soybean plants, but it increased the degree of resistance to the silverleaf whitefly. We conclude that silicon applications combined with cultivar IAC-19 can significantly decrease silverleaf whitefly populations, having a positive impact both on the soybean plant and on the environment.  相似文献   

9.
沈媛  金桂华  任顺祥  杜予州  邱宝利 《昆虫学报》2009,52(10):1132-1138
烟粉虱Bemisia tabaci是一个复合种,它具有的生物型分化、较强的传播病毒的能力和抗药性、较快的繁殖速率等特征使其成为我国农业生产中重要害虫之一。本研究利用细胞色素线粒体氧化酶Ⅰ基因,对采集自江苏、广东和云南三省的烟粉虱样本进行了生物型鉴定,并对烟粉虱生物型与寄主植物之间的关联性开展了调查。结果表明,在广东和云南省,都存在未鉴定的土著种群与入侵的B型、Q型共存的现象;同时,在本研究中广东省尚未采集到Q型烟粉虱,而在江苏采集到的粉虱样本全部为入侵型。研究结果还表明,相对于入侵种而言,土著种群显示出更强的寄主植物趋同性;丰富的寄主植物以及本身具有的多食性特性有助于B型、Q型等生物型在世界各地的广泛入侵。  相似文献   

10.
Abstract:  Bemisia tabaci (Gennadius) is an important pest of agriculture and horticulture crops that includes many morphologically indistinguishable biotypes. Molecular markers can provide a scientific method to rapidly identify biotypes. In this study, we attempted to develop specific primer sets for rapidly and stably identifying various biotypes of B. tabaci . We developed sequence characterized amplified region (SCAR) markers for each of six B. tabaci (Gennadius) biotypes (A, B, Q, Nauru, An and S). Two primer sets (BaAF/BaAR and BaQF/BaQR) were designed from random amplified polymorphic DNA-specific fragments for the A and Q biotypes. Four forward primers, BaBF, BaNaF, BaANF and BaSF, with a common reverse primer, L2-N-3014, for the other four biotypes of B, Nauru, An and S, respectively, were used based on their individual mitochondrial cytochrome oxidase I sequences. Six of these SCARs were useful in distinguishing each biotype from the others. Application of these SCARs will be helpful in rapidly identifying biotypes for quarantine pest control to prevent the invasion of the various biotypes.  相似文献   

11.
烟粉虱生物型研究进展   总被引:11,自引:0,他引:11  
褚栋  毕玉平  张友军  娄蕴萍 《生态学报》2005,25(12):3398-3405
烟粉虱B em isia tabaci(G ennad ius)是一种取食植物汁液的重要农业害虫,同时也是许多植物病毒最重要的传播介体之一。烟粉虱被认为是由许多具有明显遗传分化的不同种群即生物型组成的复合种,其中B型烟粉虱是一种入侵性最强的生物型,几乎在世界各地均有分布。由于不同生物型的烟粉虱在寄主范围、传毒能力、地理分布、抗药性等许多生物学方面存在差异,因此对烟粉虱生物型的鉴定及其遗传分化研究对于该害虫的可持续控制具有重要的指导意义。烟粉虱生物型的鉴定方法包括生物学鉴定、酶谱鉴定以及分子标记鉴定的方法,其中使用的分子标记包括RAPD、AFLP、rDNA-ITS1、m tDNA CO I以及SSR等标记。目前,不同生物型的烟粉虱尤其B型烟粉虱的分类地位仍然存在争议。介绍了不同生物型烟粉虱的生物学差异、鉴定方法及其遗传分化研究的最新进展,同时探讨了不同生物型烟粉虱的分类地位和研究方向。  相似文献   

12.
The whitefly, Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae), is generally considered to have originated from the Indian subcontinent, although little information has so far been collected on the molecular diversity of populations present in this region. The genetic diversity of B. tabaci populations from Karnataka State, south India was analysed using the random amplified polymorphic DNA‐polymerase chain reaction (RAPD‐PCR) technique and partial mitochondrial cytochrome oxidase I (mtCOI) gene sequences (689 bases) of 22 selected samples. A total of 108 whitefly samples analysed by RAPD‐PCR produced 89 polymorphic bands, and cluster analyses grouped them according to their geographic origin into ‘north’ and ‘south’ Karnataka. Phylogenetic analysis of mtCOI gene sequences with reference B. tabaci sequences from other Asian countries divided them into three genotypic clusters. Each cluster was supported with high bootstrap values (82–100%) and the individuals belonging to each cluster shared high nucleotide identities (up to 100%). This indicated at least three distinct genotypes, apparently indigenous to India, which are also present in China, Malaysia, Nepal, Pakistan, and Thailand. These coexist with the B biotype, which was first reported in India in 1999, and has since spread rapidly to other states in south India. The B biotype was more common than the indigenous B. tabaci, in locations where it had been present for more than 2 years. This is reminiscent of the situation in the Americas during the early 1990s, where the B biotype replaced existing biotypes and caused unprecedented losses to agriculture.  相似文献   

13.
烟粉虱生物型的监测及其遗传结构研究   总被引:2,自引:1,他引:1  
褚栋  张友军  万方浩 《昆虫知识》2008,45(3):353-356
烟粉虱Bemisia tabaci(Gennadius)是一种重要的农业害虫并包括许多生物型,其中B型和Q型是入侵性较强的2种生物型。文章着重介绍近年来在烟粉虱生物型的监测及其遗传结构方面的研究进展。B型烟粉虱和Q型烟粉虱这2个生物型均已入侵我国,其中多数地区烟粉虱是B型烟粉虱,局部地区有Q型烟粉虱并呈现不断蔓延趋势。微卫星(SSR)分子标记分析结果表明我国B型烟粉虱的入侵来源具有多元化,而云南地区Q型烟粉虱来源比较单一。化学农药的使用能够影响室内种群的遗传结构,降低种群的遗传多样性。基于RAPD、ISSR分子标记的分析结果表明,Q型烟粉虱种群各项遗传多样性指数均比B型烟粉虱的高。今后加强烟粉虱入侵生物型的遗传结构及其种群动态关系等方面的研究,对于揭示烟粉虱的灾变机制及其控制具有重要的意义。  相似文献   

14.
本文连续5年系统监测了江苏省13个地级市烟粉虱Bemisia tabaci(Gennadius)生物型的发生分布状况,探讨了它们的迁移扩散和演替规律。利用RAPD分子标记和mtDNA COI基因序列进行烟粉虱生物型鉴定,结果表明:江苏地区发生的烟粉虱生物型为B型和Q型。自2005年到2009年,B型烟粉虱在苏南和苏北地区的发生分布频率逐年下降,在苏北地区的发生分布频率由52.58%下降为22.22%,在苏南由56.52%下降为9.37%;而Q型烟粉虱在苏南和苏北的发生分布频率逐年升高,在苏北由47.42%上升至77.78%,在苏南由43.48%上升为90.63%。B型烟粉虱的发生分布范围由全省逐渐向苏北地区缩小,而Q型烟粉虱的发生分布范围逐渐扩大遍及全省;此外,Q型烟粉虱在江苏是由苏中和苏南地区向苏北地区扩散,并逐步取代B型烟粉虱成为江苏地区农作物的主要害虫。  相似文献   

15.
Jiu M  Zhou XP  Tong L  Xu J  Yang X  Wan FH  Liu SS 《PloS one》2007,2(1):e182
The relationships between plant viruses, their herbivore vectors and host plants can be beneficial, neutral, or antagonistic, depending on the species involved. This variation in relationships may affect the process of biological invasion and the displacement of indigenous species by invaders when the invasive and indigenous organisms occur with niche overlap but differ in the interactions. The notorious invasive B biotype of the whitefly complex Bemisia tabaci entered China in the late 1990s and is now the predominant or only biotype in many regions of the country. Tobacco curly shoot virus (TbCSV) and Tomato yellow leaf curl China virus (TYLCCNV) are two whitefly-transmitted begomoviruses that have become widespread recently in south China. We compared the performance of the invasive B and indigenous ZHJ1 whitefly biotypes on healthy, TbCSV-infected and TYLCCNV-infected tobacco plants. Compared to its performance on healthy plants, the invasive B biotype increased its fecundity and longevity by 12 and 6 fold when feeding on TbCSV-infected plants, and by 18 and 7 fold when feeding on TYLCCNV-infected plants. Population density of the B biotype on TbCSV- and TYLCCNV-infected plants reached 2 and 13 times that on healthy plants respectively in 56 days. In contrast, the indigenous ZHJ1 performed similarly on healthy and virus-infected plants. Virus-infection status of the whiteflies per se of both biotypes showed limited effects on performance of vectors on cotton, a nonhost plant of the viruses. The indirect mutualism between the B biotype whitefly and these viruses via their host plants, and the apparent lack of such mutualism for the indigenous whitefly, may contribute to the ability of the B whitefly biotype to invade, the displacement of indigenous whiteflies, and the disease pandemics of the viruses associated with this vector.  相似文献   

16.
本文报道海氏桨角蚜小蜂Erelmocerus hayati在室内对不同寄主密度下的B型和Q型烟粉虱Bemisiatabaei寄生和取食情况,并比较了该蜂对两种生物型烟粉虱功能反应的差异。结果表明,海氏桨角蚜小蜂对两种生物型烟粉虱的寄生率和致死率可分别达到60%和70%以上,平均每日最大致死量可达40头烟粉虱若虫。该蜂对两种生物型烟粉虱的功能反应曲线符合Holling Ⅱ型,随寄主密度的增加对烟粉虱若虫的寄生和致死数量均增加直至达到每日最大致死数量,而寄生率和致死率则随寄主密度的增加而显著下降。该蜂对两种生物型烟粉虱的功能反应没有显著的差异。  相似文献   

17.
烟粉虱的分类地位及在中国的分布   总被引:9,自引:0,他引:9       下载免费PDF全文
烟粉虱广泛分布于全球热带和亚热带地区。近20多年,烟粉虱的一些遗传群入侵世界各地,严重危害作物生产。烟粉虱遗传结构的多样性和复杂性早已被关注,但其分类地位,尤其是烟粉虱到底是一个包含多个生物型的种还是一个包含许多隐种的物种复合体,一直颇受争议。近几年,有关烟粉虱种系发生和系统学的研究取得长足进展,有证据推论其是一个包含至少31个隐种的物种复合体,但生殖隔离证据仍显不足,种系发生分析结果也因仅依据COI一个基因而受到质疑。因此,在大多数从事烟粉虱研究的同行接受其为一个物种复合体的概念的同时,仍有同行沿用生物型的概念。在我国境内已先后报道了包括13个本地种和2个全球入侵种在内的15个烟粉虱隐种。本地种主要分布在我国南部及包括海南岛和台湾岛的东南沿海地区,隐种的多样性由南向北逐渐降低。入侵种“中东一小亚细亚1”隐种(MEAMl)(即“B型”)和“地中海”隐种(MED)(即“Q型”)分别于20世纪90年代中后期和2003年前后入侵我国,并在许多地区迅速取代了本地种而占据优势地位。全国范围内的调查数据显示,这2个入侵种可在大部分区域共同存在,但自2005年以来,MED在许多地区陆续取代MEAMl,这很可能与MED对大量使用的新烟碱类杀虫剂有较强抗性有关。本文还讨论了烟粉虱隐种复合体分类所面临的命名等难题以及大范围抽样调查的数据偏差问题。  相似文献   

18.
In an attempt to identify a specific marker for biotype 2 of the Asian rice gall midge (Orseolia oryzae, Wood-Mason), we used AFLP (amplified fragment length polymorphism) fingerprinting. We identified an AFLP marker that is specifically amplified in biotypes 1, 2 and 5 of the rice gall midge, but not in biotype 4. Biotypes 1, 2 and 5 are avirulent to hosts bearing the Gm2 resistance gene (found in rice variety Phalguna), whereas biotype 4 is virulent to Gm2. Based on the sequence of this AFLP marker, SCAR (sequence characterized amplified region) primers were designed and used in combination with previously developed SCAR primers to distinguish effectively all five biotypes in a multiplex PCR-based assay. The inheritance pattern of this marker in the progenies of inter-biotype crosses between biotypes 1, 2 and 4 shows that the marker can be amplified by PCR from all F1 females, irrespective of the biotype status of their parents. However, the marker is present only in those male progenies whose mother was of a Gm2 avirulent biotype. The specific amplification of this marker in the avirulent biotypes and its pattern of inheritance show that avirulence with respect to carriers of the Gm2 gene in rice gall midge is sex-linked. Received: 16 August 1999 / Accepted: 27 December 1999  相似文献   

19.
Wolbachia是专性的细胞内细菌,广泛存在于节肢动物生殖组织。已有的研究结果表明,节肢动物中存在A组和B组Wolbachia,而烟粉虱Bemisia tabaci中主要检测到了B组Wolbachia。本研究从福建省采集到17个不同烟粉虱地理种群,首先通过rDNA-ITS1克隆测序鉴定了不同烟粉虱地理种群的生物型,然后采用Wolbachia 16S rDNA的特异引物,并通过PCR-RFLP技术分析了不同烟粉虱地理种群中Wolbachia的感染特点。结果表明:从福建省闽侯、平潭、南平、来舟、漳平和沙县采集到的烟粉虱自然种群属于非B型,而非B型烟粉虱种群中存在广泛的超感染现象,即单个非B型烟粉虱个体中同时感染了不同型Wolbachia。相反,B型烟粉虱自然种群的个体中只感染A组Wolbachia。该研究依据密集采样的数据进一步证实了Wolbachia在烟粉虱自然种群中的分布确实与宿主的生物型密切相关,提示Wolbachia可能在烟粉虱的种群分化中发挥作用。  相似文献   

20.
为了揭示辽宁省冬季温室内越冬粉虱伪蛹的种类及烟粉虱Bemisia tabaci (Gennadius)携带番茄黄化曲叶病毒(tomato yellow leaf curl virus, TYLCV)情况, 于2012年1月份在辽宁省不同县市区的温室作物上采集了17份粉虱伪蛹样品(每样品含30头粉虱伪蛹) , 镜检鉴别粉虱种类并利用mtCOI基因对烟粉虱生物型进行了鉴定; 检测了烟粉虱携带TYLCV情况并对其PCR扩增产物进行了测序分析。结 果表明: 辽宁省冬季温室内存在越冬温室白粉虱Trialeurodes vaporariorum (Westwood)与烟粉虱。17份粉虱样品中, 11份样品为烟 粉虱样品, 6份样品为温室白粉虱和烟粉虱混合样品。混合样品中, 温室白粉虱仅在锦州凌海(LH)样品中占优势。17份烟粉虱样品(包 括混合样品)中, 仅有4份样品为B型与Q型混合样品, 其他13份样品烟粉虱生物型均为Q型。17份烟粉虱样品中有3份Q型烟粉虱样品检测 到TYLCV, 系统树分析进一步证实该病毒是TYLCV。调查结果为辽宁省粉虱与TYLCV的早期测报和防控提供了科学依据。  相似文献   

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