缺血性脑损伤与程序性死亡

脑缺血后细胞会产生程序性死亡（ｐｒｏｇｒａｍｍｅｄ ｃｅｌｌ ｄｅａｔｈ,ＰＣＤ）,并参与脑损伤;神经元的迟发性死亡属于ＰＣＤ。脑缺血后细胞内钙增加,一氧化氮的生产、脂质过氧化反应、Ｐ５３基因的表达等,在程序性死亡的形成中具有重要作用。     

Morphological criteria to distinguish cell death induced by apoptotic and necrotic treatments

We present a comparative study of apoptotic and necrotic morphology (light and scanning electron microscopy), induced by well known experimental conditions (photodynamic treatments, etoposide, hydrogen peroxide, freezing-thawing and serum deprivation) on cell cultures. Our results indicate that morphological criteria (apoptotic cell rounding and shrinkage, and appearance of membrane bubbles in early necrosis) allow to distinguish these cell death mechanisms, and also show that, independently of the damaging agents, the necrotic process occurs in a characteristic sequence (coalescence of membrane bubbles in a single big one that detaches from cells remaining on the substrate).     

Diagnostic criteria for diabetes revisited: making use of combined criteria

Background  

Existing cut-offs for fasting plasma glucose (FPG) and post-load glucose (2hPG) criteria are not equivalent in the diagnosis of diabetes and glucose intolerance. Adjusting cut-offs of single measurements have not helped so we undertook this project to see if they could be complementary.