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1.
Diurnal Changes in Metabolite Levels and Crassulacean Acid Metabolism in Kalanchoë daigremontiana Leaves
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Diurnal changes in levels of selected metabolites associated with glycolysis, the C3 cycle, C4-organic acids, and storage carbohydrates were analyzed in active Kalanchoë daigremontiana Crassulacean acid metabolism leaves. Three metabolic transition periods occurred each day. During the first two hours of light, nearly all of the metabolite pools underwent transient changes. Beginning at daylight, stomata opened transiently and closed again within 30 minutes; malate synthesis continued for about 1 hour into the light; C3 photosynthesis began within 30 minutes; and net quantities of starch and glucan began to accumulate after 2 hours, continuing linearly throughout the rest of the day. 相似文献
2.
Changes in Metabolite Levels in Kalanchoë daigremontiana and the Regulation of Malic Acid Accumulation in Crassulacean Acid Metabolism
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Changes in glucose-6-P, fructose-6-P, fructose-1,6-diP, 6-phospho-gluconate, phosphoenolpyruvate, 3-phosphoglycerate, and pyruvate levels in the leaves of the Crassulacean plant Kalanchoë daigremontiana Hammet et Perrier were measured enzymically during transitions from CO2-free air to air, air to CO2-free air, and throughout the course of acid accumulation in darkness. The data are discussed in terms of the involvement of phosphoenolpyruvate carboxylase in malic acid synthesis and in terms of the regulation of the commencement of malic acid synthesis and accumulation through the effects of CO2 on storage carbohydrate mobilization and its termination through the effects of malic acid on phosphoenolpyruvate carboxylase activity. 相似文献
3.
Nitrate Assimilation and Crassulacean Acid Metabolism in Leaves of Kalanchoë fedtschenkoi Variety Marginata
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Chang NK 《Plant physiology》1981,68(2):464-468
The enzymes necessary to assimilate ammonia either via glutamine synthetase and glutamate synthase or via the glutamate dehydrogenase pathways are present in both green and white leaf tissues of Kalanchoë fedtschenkoi. Nitrate reductase activity develops to a maximum in a Crassulacean acid metabolism (CAM) plant canopy before either ribulose 1,5-bisphosphate carboxylase, or phosphoenolpyruvate carboxylase, or CAM. Nitrate reductase also is activated each morning and is inactivated late in the day as in other plants. However, there does not appear to be any direct relationship between nitrate reductase activity and the level of acid, its daily pattern or the amplitude of CAM. Though nitrate reductase is activated maximally each day by light, in Kalanchoë leaves for six days the activity followed a precise daily pattern independent of continuous light or dark. 相似文献
4.
Malate Decarboxylation by Kalanchoë daigremontiana Mitochondria and Its Role in Crassulacean Acid Metabolism
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Day DA 《Plant physiology》1980,65(4):675-679
Mitochondria isolated from Kalanchoë daigremontiana, a Crassulacean acid metabolism plant, decarboxylate added malate to pyruvate at rates of up to 100 micromoles per hour per milligram original chlorophyll in the presence of ADP. Omitting ADP reduces this rate by approximately 50%. Antimycin A inhibits malate decarboxylation and this inhibition could be relieved by addition of aspartate and α-ketoglutarate to the mitochondria. Increasing the pH of the external medium inhibited malate decarboxylation; a dramatic decrease in pyruvate production was observed between pH 7.2 and pH 7.4. It is suggested that cytoplasmic pH changes may regulate the contribution of mitochondria to malate decarboxylation in the light in vivo. 相似文献
5.
Water-relation Parameters of Individual Mesophyll Cells of the Crassulacean Acid Metabolism Plant Kalanchoë daigremontiana
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Steudle E 《Plant physiology》1980,66(6):1155-1163
Water-relation parameters of leaf mesophyll cells of the CAM plant Kalanchoë daigremontiana have been determined directly in cells of tissue slices using the pressure-probe technique. Turgor pressures measured in cells of the second to fourth layer from the cut surface showed an average of 1.82 ± 0.62 bar (mean ± sd; n = 157 cells). This was lower than expected from measurements of the osmotic pressure of the cell sap. The half-time (T1/2) for water-flux equilibration of individual cells was 2.5 to 8.8 seconds. This is the fastest T1/2 found so far for higher-plant cells. The calculated values of the hydraulic conductivity were in the range of 0.20 to 1.6 × 10−5 centimeters second−1 bar−1, with an average of (0.69 ± 0.46) × 10−5 centimeters second−1 bar−1 (mean ± sd; n = 8 cells). The T1/2 values of water exchange of individual cells are consistent with the overall rates of water-flux equilibration measured for tissue slices.The volumetric elastic moduli (∈) of individual cells were in the range 13 to 128 bar for turgor pressures between 0.0 and 3.4 bar; the average ∈ value was 42.4 ± 27.7 bar (mean ± sd; n = 21 cells). This ∈ value is similar to that observed for other higher-plant cells.The water-storage capacity of individual cells, calculated as Cc = V/(∈ + πi) (where V = cell volume and πi = internal osmotic pressure) was 9.1 × 10−9 cubic centimeters bar−1 per cell, and the capacity for the tissue was 2.2 × 10−2 cubic centimeters bar−1 gram−1 fresh weight. The significance of the water-relation parameters determined at the cellular level is discussed in terms of the water relations of whole leaves and the high water-use efficiency characteristic of CAM plants. 相似文献
6.
Modulation of Rubisco Activity during the Diurnal Phases of the Crassulacean Acid Metabolism Plant Kalanchoë daigremontiana
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Maxwell K Borland AM Haslam RP Helliker BR Roberts A Griffiths H 《Plant physiology》1999,121(3):849-856
The regulation of Rubisco activity was investigated under high, constant photosynthetic photon flux density during the diurnal phases of Crassulacean acid metabolism in Kalanchoë daigremontiana Hamet et Perr. During phase I, a significant period of nocturnal, C4-mediated CO2 fixation was observed, with the generated malic acid being decarboxylated the following day (phase III). Two periods of daytime atmospheric CO2 fixation occurred at the beginning (phase II, C4–C3 carboxylation) and end (phase IV, C3–C4 carboxylation) of the day. During the 1st h of the photoperiod, when phosphoenolpyruvate carboxylase was still active, the highest rates of atmospheric CO2 uptake were observed, coincident with the lowest rates of electron transport and minimal Rubisco activity. Over the next 1 to 2 h of phase II, carbamylation increased rapidly during an initial period of decarboxylation. Maximal carbamylation (70%–80%) was reached 2 h into phase III and was maintained under conditions of elevated CO2 resulting from malic acid decarboxylation. Initial and total Rubisco activity increased throughout phase III, with maximal activity achieved 9 h into the photoperiod at the beginning of phase IV, as atmospheric CO2 uptake recommenced. We suggest that the increased enzyme activity supports assimilation under CO2-limited conditions at the start of phase IV. The data indicate that Rubisco activity is modulated in-line with intracellular CO2 supply during the daytime phases of Crassulacean acid metabolism. 相似文献
7.
C Nuclear Magnetic Resonance Studies of Crassulacean Acid Metabolism in Intact Leaves of Kalanchoë tubiflora
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13C nuclear magnetic resonance spectroscopy of intact leaves of Kalanchoë tubiflora was used to observe Crassulacean acid metabolism in vivo. 13C signals from C-4 of malate were observed after overnight exposure of leaves to 13CO2. Illumination of the labeled leaves resulted in a gradual decrease in the malate signals. After a period of darkness in normal air, 13C signals were detected in all four carbons of malate in the previously labeled leaves. The 13C nuclear magnetic resonance spectrum of malate in solution was pH dependent, which allowed an estimation of the vacuolar pH from the whole leaf spectrum. The pH was 4.0 following a 14-hour dark period, but rose to greater than 6.0 after 6 hours of illumination. 相似文献
8.
Light Scattering as an Indicator of the Energy State in Leaves of the Crassulacean Acid Metabolism Plant Kalanchoë pinnata 总被引:2,自引:2,他引:0
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Both transmittance changes in a weak beam of green light (light scattering) and the slow decay of chlorophyll a fluorescence were used as indicators of the energy state of leaves of a Crassulacean acid metabolism plant, Kalanchoë pinnata, at frequent intervals during 12-hour light/12-hour dark cycles. To induce light scattering and fluorescence changes, leaves were exposed to red light for 6 minutes. When measurements were made during the light period, the leaves were kept in darkness for 6 minutes before illumination. In the middle of the light period, when malic acid decarboxylation was very active and stomatal conductance was low, light scattering changes were small and indicated that the energy state of leaves was low. This result was supported by determination of adenylate levels. Light scattering and ATP/ADP ratios increased during the late light period when the tissue was deacidified. Illumination produced maximum light scattering changes between the 2nd and 5th hour of the dark period, when rates of dark CO2 fixation were highest. Light scattering and fluorescence measurements taken from leaves, which were illuminated with red or far-red light in the presence or absence of O2 showed that, in addition to linear electron transport, K. pinnata has the potential for both cyclic and pseudocyclic electron transport. The results are relevant with regard to the high ATP demand during Crassulacean acid metabolism. 相似文献
9.
Reduction State of Q and Nonradiative Energy Dissipation during Photosynthesis in Leaves of a Crassulacean Acid Metabolism Plant, Kalanchoë daigremontiana Hamet et Perr 总被引:2,自引:2,他引:0
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Fluorescence was measured in leaves of the CAM plant Kalanchoë daigremontiana using a pulse modulation technique at room temperature. During a 12-h light period at 500 micromole photons per square meter per second (400-700 nanometers) in air containing 350 microbar CO2, the component of fluorescence quenching related to the reduction state of Q, the primary electron transport acceptor of PSII, remained fairly constant and showed that only 20% of Q were in the reduced form. The reduction state was slightly increased at the onset and at the end of the light period. By contrast, the nonphotochemical component of fluorescence quenching which is a measure of the fraction of nonradiative deexcitation underwent marked diurnal changes. Nonradiative energy conversion was low during the phase of most active malic acid decarboxylation in the middle of the light period when uptake of atmospheric CO2 was negligible, and when internal CO2 partial pressures were higher than in air; this allowed for high rates of CO2 reduction in the chloroplasts. Nonradiative energy conversion was high during the early and the late light period when atmospheric CO2 was taken up and internal CO2 partial pressures were below air level. Manipulation of the internal CO2 partial pressure during the late light period by increasing or decreasing the external CO2 partial pressure to 1710 and 105 microbar, respectively, led to changes in the magnitude of energy dependent fluorescence quenching which were consistent with the relationship between nonradiative energy dissipation and internal CO2 partial pressure observed during the diurnal cycle. Again, the reduction state of Q was hardly affected by these treatments. Thus, changes in electron transport rate during the diurnal CAM cycle at a given photon flux density lead primarily to alterations in the rate of nonradiative energy dissipation, with the reduction state of Q being maintained at a relatively low and constant level. Conditions are described under which nonphotochemical dissipation of excitation energy reaches a maximum value and the reduction state of Q is increased. 相似文献
10.
Nicotinamide Adenine Dinucleotide-specific "Malic" Enzyme in Kalanchoë daigremontiana and Other Plants Exhibiting Crassulacean Acid Metabolism
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Dittrich P 《Plant physiology》1976,57(2):310-314
NAD-specific “malic” enzyme (EC 1.1.1.39) has been isolated and purified 1200-fold from leaves of Kalanchoë daigremontiana. Kinetic studies of this enzyme, which is activated 14-fold by CoA, acetyl-CoA, and SO42−, suggest allosteric properties. Cofactor requirements show an absolute specificity for NAD and for Mn2+, which cannot be replaced by NADP or Mg2+. For maintaining enzyme activity in crude leaf extracts a thiol reagent, Mn2+, and PVP-40 were required. The latter could be omitted from purified preparations. By sucrose density gradient centrifugation NAD-malic enzyme could be localized in mitochondria. A survey of plants with crassulacean acid metabolism revealed the presence of NAD-malic enzyme in all 31 plants tested. Substantial levels of this enzyme (121-186 μmole/hr·mg of Chl) were detected in all members tested of the family Crassulaceae. It is proposed that NAD-malic enzyme in general supplements activity of NADP-malic enzyme present in these plants and may be specifically employed to increase internal concentrations of CO2 for recycling during cessation of gas exchange in periods of severe drought. 相似文献
11.
Measurements of the Engagement of Cyanide-Resistant Respiration in the Crassulacean Acid Metabolism Plant Kalanchoë daigremontiana with the Use of On-Line Oxygen Isotope Discrimination
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Robinson SA Yakir D Ribas-Carbo M Giles L Osmond CB Siedow JN Berry JA 《Plant physiology》1992,100(3):1087-1091
Discrimination against 18O during dark respiration in tissues of Kalanchoë daigremontiana, Medicago sativa, and Glycine max was measured using an on-line system that enabled direct measurements of the oxygen fractionation of samples in a gas-phase leaf disk electrode unit. Discrimination factors for cytochrome pathway respiration were 18.6 to 19.8%o for all tissues. However, discrimination in cyanide-resistant respiration was significantly higher in green tissues (30.4-31.2%o) compared with nongreen tissues (25.3-25.9%o). Using these discrimination factors, the partitioning of electron transport to these pathways was calculated from measurements of discrimination in the absence of inhibitors. Changes in flux through the alternative pathway were measured during the light and dark phases of Crassulacean acid metabolism in leaf disks of K. daigremontiana. The flux of electrons through the alternative pathway was higher during deacidification than during the other phases of Crassulacean acid metabolism. The increase in alternative pathway electron flux accounted for all of the increased respiration in the light phase. Despite this increase, simultaneous measurements of malate concentration and respiratory flux confirm that only a small proportion of the total malate decarboxylation occurs in the mitochondria. 相似文献
12.
Malate Metabolism in Leaf Mitochondria from the Crassulacean Acid Metabolism Plant Kalanchoë blossfeldiana Poelln
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The mechanisms and the controlling factors of malate oxidation by mitochondria from leaves of Kalanchoë blossfeldiana Poelln. plants performing Crassulacean acid metabolism were investigated using Percollpurified mitochondria. The effects of pH and of various cofactors (ATP, NAD+, coenzyme A) on malate dehydrogenase (EC 1.1.1.37) and malic enzyme (EC 1.1.1.39) solubilized from these mitochondria were examined. The crucial role of cofactor concentrations in the mitochondrial matrix on the pathways of malate oxidation is shown. The distribution of the electrons originating from malate between the different electron transport pathways and its consequence on the phosphorylation yield was studied. It was found that, depending on the electron transport pathway used, malate oxidation could yield from 3 to 0 ATP. Assayed under conditions of high reducing power and high energy charge, the ability of malic enzyme to feed electrons to the cyanide-resistant nonphosphorylating alternative pathway was found to be higher than that of other dehydrogenases linked to the functioning of the Krebs cycle (pyruvate dehydrogenase, isocitrate dehydrogenase, α-ketoglutarate dehydrogenase, succinate dehydrogenase). The physiological significance of such a functional relationship between malic enzyme activity and the nonphosphorylating alternative pathway is discussed in relation to Crassulacean acid metabolism. 相似文献
13.
Light-Stimulated Burst of Carbon Dioxide Uptake following Nocturnal Acidification in the Crassulacean Acid Metabolism Plant Kalanchoë diagremontiana 总被引:1,自引:1,他引:0
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CO2 exchange characteristics were studied during the light-stimulated burst of CO2 uptake (MB) immediately following a period of nocturnal CO2 fixation in the Crassulacean acid metabolism plant Kalanchoë daigremontiana. During the early parts of the MB, stimulation of net CO2 uptake by low ambient O2 concentration (1.5%) was small, and leaves showed the capacity for net CO2 uptake at low ambient CO2 partial pressure (30 microbars) and when the MB was interrupted by darkness. During the later phase of the MB, stimulation of net CO2 uptake by 1.5% O2 was increased, and net CO2 loss was recorded both at 30 microbars CO2 and during dark interruptions. These results suggest that CO2 fixation during the MB occurs simultaneously via phosphoenolpyruvate carboxylase (predominant during the early phase of the MB) and via ribulose bisphosphate carboxylase (predominant during the later phase of the burst). The magnitude and duration of the MB was increased by a reduction in the length of the dark period and by low (15°C) compared to high (30°C) leaf temperatures. 相似文献
14.
Occurrence of Pyruvate Orthophosphate Dikinase in the Succulent Plant, Kalanchoë daigremontiana Hamet. et. Perr
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Sugiyama T 《Plant physiology》1975,56(5):605-607
Pyruvate orthophosphate dikinase was detected from Kalanchoë daigremontiana Hamet. et. Perr., a succulent plant with crassulacean acid metabolism. Enzyme activity was similar to that of maize extracts. Two enzymes demonstrating pyruvate orthophosphate dikinase activity from K. daigremontiana and Zea mays were found to be partially identical from enzyme-inhibition and immunoprecipitin tests with maize enzyme antiserum. A time course study demonstrated that pyruvate orthophosphate dikinase activity in leaf extracts was dependent upon exposure of leaves to light. 相似文献
15.
Tonoplast vesicles were prepared from leaf mesophyll homogenates of the crassulacean-acid-metabolism plant Kalanchoë daigremontiana Hamet et Perrier de la Bâthie to study the effects of anions on ATP- and inorganic-pyrophosphate (PPi)-dependent H+ transport. In the presence of gramicidin, substrate hydrolysis by the tonoplast ATPase was characteristically stimulated by chloride and inhibited by nitrate, but was unaffected by malate and a wide range of other organic-acid anions; the PPiase was anion-insensitive. Malate was more effective than chloride both in stimulating ATP- and PPi-dependent vesicle acidification (measured as quinacrine-fluorescence quenching) and in dissipating a pre-existing inside-positive membrane potential (measured as oxonol-V-fluorescence quenching), indicating that malate was more readily transported across the tonoplast. Certain other four-carbon dicarboxylates also supported high rates of vesicle acidification, their order of effectiveness being fumarate malate -succinate > oxalacetate - tartrate; the five-carbon dicarboxylates 2-oxoglutarate and glutarate were also transported, although at lower rates. Experiments with non-naturally occurring anions indicated that the malate transporter was not stereospecific, but that it required the trans-carboxyl configuration for transport. Shorter-chain or longer-chain dicarboxylates were not transported, and neither were monocarboxylates, the amino-acid anions aspartate and glutamate, nor the tricarboxylate isocitrate. The non-permeant anions maleate and tartronate appeared to be competitive inhibitors of malate transport but did not affect chloride transport, indicating that malate and chloride influx at the tonoplast might be mediated by separate transporters.Abbreviations BTP
1,3-bis-[tris(hydroxymethyl)methylamino]-propane
- CAM
crassulacean acid metabolism
- oxonol V
bis(3-phenyl-5-oxoisoxazol-4-yl)pentamethine oxonol
- pH
transmembrane pH difference
- PPi
inorganic pyrophosphate
- PPiase
inorganic pyrophosphatase
- quinacrine
6-chloro-9-{[4-(diethylamino)-1-methylbutyl]amino}-2-methoxyacridine dihydrochloride
-
transmembrane electrical potential difference 相似文献
16.
Malate Metabolism in the Dark After CO(2) Fixation in the Crassulacean Plant Kalanchoë tubiflora
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The metabolism of [13C]malate was studied in the Crassulacean plant Kalanchoë tubiflora following exposure to 13CO2 for 2 hour intervals during a 16 hour dark cycle. Nuclear magnetic resonance spectroscopy of [13C]malate extracted from labeled tissue revealed that the transient flux of malate to the mitochondria, estimated by the randomization of [4-13C]malate to [1- 13C]malate by fumarase, varied substantially during the dark period. At both 15 and 25°C, the extent of malate label randomization in the mitochondria was greatest during the early and late parts of the dark period and was least during the middle of the night, when the rate of 13CO2 uptake was highest. Randomization of labeled malate continued for many hours after malate synthesis had initially occurred. Internally respired 12CO2 also served as a source of carbon for malate formation. At 15°C, 15% of the total malate was formed from respired 12CO2, while at 25°C, 49% of the accumulated malate was derived from respired 12CO2. Some of the malate synthesized from external 13CO2 was also respired during the night. The proportion of the total [13C]malate respired during the dark period was similar at 15 and 25°C, and respiration of newly formed [13C]malate increased as the night period progressed. These data are discussed with regard to the relative fluxes of malate to the mitochondria and the vacuole during dark CO2 fixation. 相似文献
17.
The degradation of starch by a protein fraction of Kalanchoë daigremontiana Hamet et Perrier, obtained by ammoniumsulfate precipitation (30–70%), was found to be catalyzed by -and -amylase (EC 3.2.1.1 and EC 3.2.1.2, respectively) and by starch phosphorylase (EC 2.4.1.1). The activity of these enzymes was determined by chromatographic analysis of the reaction products; separation and identification of -amylase was accomplished by heat-inactivation of -amylase and -glucosidase. When the interaction of amylolytic and phosphorolytic enzymes was comparatively studied, it was found that without inorganic phosphorus in the reaction mixture, 14C-starch was converted predominantly to maltose and glucose; supplementation with 1–10 mM orthophosphate (Pi) resulted in an increase in glucose-1-phosphate formation and a concomitant reduction of maltose production. Since the total volume of starch degradation remained approximately constant, Pi apparently inhibits -amylase (Ki about 3 mM Pi). Thus, free Pi in the cell participates in the regulation of starch catabolism, serving as a substrate for starch phosphorylase while simultaneously reducing the production of maltose. With respect to glucan synthesis, adenosinediphosphoglucose--1,4-glucosyltransferase (EC 2.4.1.22), maltose phosphorylase and maltoseglucosyltransferase were also found to be active. The last-named enzyme catalyzes an exchange between dextrins and is considered to provide primer carbohydrates for the synthesis of polyglucans.Abbreviations ADPG
adenosinediphosphoglucose
- G1P
glucose-1-phosphate
- PEG
polyethylenglycol
- PEP
phosphoenolpyruvate
- Pi
orthophosphate 相似文献
18.
With a prerequisite of 5 hours in the light the detached leaves of wheat seedling and detached leaves and cotyledons of sunflower were able to utilize exogenous γ-aminobutyric acid, the amount of alanine and glutamine formed were increased. It was found that deamination of γ-aminobutyric acid was associated with γ-aminobutyric-pyruvate transaminase in the leaves of wheat seedling. The specific activity of this transaminase was enhanced about 5.6 fold by adsorption with calcium phosphate gel-Some properties of this transaminas were as follows: the optimum pH was 8.9. The produced amount of alanine was linear with time up to a period of one hour. The primary rate of reaction was proportional to enzyme concentration in the first hour. The relation of substrate concentration to the primary rate of alanine formation was determinated and Michaelis constant was evaluated about 2.6 × 10-3 M. The action of this transaminase in metabolism of γ-aminobutyrie acid was discussed. 相似文献
19.
20.
Effect of Varying CO(2) Partial Pressure on Photosynthesis and on Carbon Isotope Composition of Carbon-4 of Malate from the Crassulacean Acid Metabolism Plant Kalanchoë daigremontiana Hamet et Perr
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Intact leaves of Kalanchoë daigremontiana were exposed to CO2 partial pressures of 100, 300, and 1000 microbars. Malic acid was extracted, purified, and degraded in order to obtain isotopic composition of carbon-1 and carbon-4. From these data, it is possible to calculate the carbon isotope composition of newly fixed carbon in malate. In all three treatments, the isotopic composition of newly introduced carbon is the same as that of the CO2 source and is independent of CO2 partial pressures over the range tested. Comparison with numerical models described previously (O'Leary 1981 Phytochemistry 20: 553-567) indicates that we would expect carbon 4 of malate to be 4‰ more negative than source CO2 if diffusion is totally limiting or 7‰ more positive than source CO2 if carboxylation is totally limiting. Our results demonstrate that stomatal aperture adjusts to changing CO2 partial pressures and maintains the ratio of diffusion resistance to carboxylation resistance approximately constant. In this study, carboxylation and diffusion resistances balance so that essentially no fractionation occurs during malate synthesis. Gas exchange studies of the same leaves from which malate was extracted show that the extent of malate synthesis over the whole night is nearly independent of CO2 partial pressure, although there are small variations in CO2 uptake rate. Both the gas exchange and the isotope studies indicate that the ratio of external to internal CO2 partial pressure is the same in all three treatments. Inasmuch as a constant ratio will result in constant isotope fractionation, this observation may explain why plants in general have fairly invariable 13C contents, despite growing under a variety of environmental conditions. 相似文献