共查询到20条相似文献,搜索用时 15 毫秒
1.
Tzyy-Bin Tsay Ming-Chieh Yang Pei-Hsuan Chen Ching-Mei Hsu Lee-Wei Chen 《Journal of biomedical science》2011,18(1):68
Background
The influence of the gut flora on lung inflammatory reaction against bacterial challenge remains undefined. This study was designed to investigate whether gut flora enhances lung defense against E.coli pneumonia through TLR4 signaling. 相似文献2.
Ling-fang He Ting-ting Wang Qian-ying Gao Guang-feng Zhao Ya-hong Huang Li-ke Yu Ya-yi Hou 《Journal of biomedical science》2011,18(1):39
Background
Stanniocalcin-1(STC-1) is up-regulated in several cancers including gastric cancer. Evidences suggest that STC-1 is associated with carcinogenesis and angiogenic process. However, it is unclear on the exact role for STC-1 in inducing angiogenesis and tumorigeneisis. 相似文献3.
Akimoto Y Miura Y Toda T Wolfert MA Wells L Boons GJ Hart GW Endo T Kawakami H 《Clinical proteomics》2011,8(1):15
Purpose
The objective of the present study is to identify proteins that change in the extent of the modification with O-linked N-acetylglucosamine (O-GlcNAcylation) in the kidney from diabetic model Goto-Kakizaki (GK) rats, and to discuss the relation between O-GlcNAcylation and the pathological condition in diabetes. 相似文献4.
Sang Won Park Xine Shen Lu-Tai Tien Richard Roman Tangeng Ma 《Journal of biomedical science》2011,18(1):83
Background
Repeated exposure to methamphetamine (METH) can cause not only neurotoxicity but also addiction. Behavioral sensitization is widely used as an animal model for the study of drug addiction. We previously reported that the μ-opioid receptor knockout mice were resistant to METH-induced behavioral sensitization but the mechanism is unknown. 相似文献5.
Jenher Lu Tzuchun Tsai Sielin Choo Shuyu Yeh Renbing Tang Anhang Yang Hsinyu Lee Jennkan Lu 《Journal of biomedical science》2011,18(1):73
Background
The tbx5 mutation in human causes Holt-Oram syndrome, an autosomal dominant condition characterized by a familial history of congenital heart defects and preaxial radial upper-limb defects. We report aberrant apoptosis and dormant cell growth over head, heart, trunk, fin, and tail of zebrafish embryos with tbx5 deficiency correspond to the dysmorphogenesis of tbx5 morphants. 相似文献6.
7.
Introduction
Programmed cell death of intervertebral disc (IVD) cells plays an important role in IVD degeneration, but the role of autophagy, a closely related cell death event, in IVD cells has not been documented. The current study was designed to investigate the effect of interleukin (IL)-1β on the occurrence of autophagy of rat annulus fibrosus (AF) cells and the interrelationship between autophagy and apoptosis. 相似文献8.
Yi-Ting Wu Che Yi Lin Ming-Yuan Tsai Yi-Hua Chen Yu-Fen Lu Chang-Jen Huang Chao-Min Cheng Sheng-Ping L Hwang 《Journal of biomedical science》2011,18(1):70
Background
β-Lapachone has antitumor and wound healing-promoting activities. To address the potential influences of various chemicals on heart development of zebrafish embryos, we previously treated zebrafish embryos with chemicals from a Sigma LOPAC1280™ library and found several chemicals including β-lapachone that affected heart morphogenesis. In this study, we further evaluated the effects of β-lapachone on zebrafish embryonic heart development. 相似文献9.
Pei Chun Lai Yen Ta Huang Chia Chen Wu Ching-Jung Lai Pen Jung Wang Ted H Chiu 《Journal of biomedical science》2011,18(1):69
Background
Perinatal brain injury is the leading cause of subsequent neurological disability in both term and preterm baby. Glutamate excitotoxicity is one of the major factors involved in perinatal hypoxic-ischemic encephalopathy (HIE). Glutamate transporter GLT1, expressed mainly in mature astrocytes, is the major glutamate transporter in the brain. HIE induced excessive glutamate release which is not reuptaked by immature astrocytes may induce neuronal damage. Compounds, such as ceftriaxone, that enhance the expression of GLT1 may exert neuroprotective effect in HIE. 相似文献10.
Mehmet Kü?ükaycan Michiel Van Krugten Herman-Jan Pennings Tom WJ Huizinga Wim A Buurman Mieke A Dentener Emiel FM Wouters 《Respiratory research》2002,3(1):29
Background
Chronic obstructive pulmonary disease (COPD) is characterized by a chronic inflammatory process, in which the pro-inflammatory cytokine Tumor Necrosis Factor (TNF)-α is considered to play a role. In the present study the putative involvement of TNF-α gene polymorphisms in pathogenesis of COPD was studied by analysis of four TNF-α gene polymorphisms in a Caucasian COPD population. 相似文献11.
12.
Fu-Ming Tsai Chang-Chieh Wu Rong-Yaun Shyu Chun-Hua Wang Shun-Yuan Jiang 《Journal of biomedical science》2011,18(1):88
Background
The tazarotene-induced gene 1 (TIG1) is a putative tumor suppressor gene. We have recently demonstrated both TIG1A and TIG1B isoforms inhibited cell growth and induced the expression of G protein-coupled receptor kinase 5 (GRK5) in colon cancer cells. Because elevated prostaglandin E2 (PGE2) signaling plays a significant role in colorectal carcinogenesis, the objective of this study was to explore the effect of TIG1 on PGE2-induced cellular proliferation and signaling in colon cancer cells. 相似文献13.
de Groot L Hinkema H Westra J Smit AJ Kallenberg CG Bijl M Posthumus MD 《Arthritis research & therapy》2011,13(6):R205
Introduction
Advanced glycation end products (AGEs) are produced and can accumulate during chronic inflammation, as might be present in patients with rheumatoid arthritis (RA). AGEs are involved in the development of cardiovascular disease. The aim of this study is to evaluate whether AGEs are increased in patients with long-standing RA and whether AGE accumulation is related to disease activity, disease severity and measures of (premature) atherosclerosis, such as endothelial activation, endothelial dysfunction and intima media thickness (IMT). 相似文献14.
Wei Zhang Hideo Tsurushima Ayako Oyane Yushin Yazaki Yu Sogo Atsuo Ito Akira Matsumura 《Journal of biomedical science》2011,18(1):62
Background
Safe and efficient gene transfer systems are needed for tissue engineering. We have developed an apatite composite layer including the bone morphogenetic protein-2 (BMP-2) gene and fibronectin (FB), and we evaluated its ability to induce bone formation. 相似文献15.
Lorenzo A Calò Luciana Bordin Paul A Davis Elisa Pagnin Lucia Dal Maso Gian Paolo Rossi Achille C Pessina Giulio Clari 《Journal of biomedical science》2011,18(1):38
Background
Angiotensin II (Ang II) signaling occurs via two major receptors which activate non-receptor tyrosin kinases that then interact with protein tyrosin-phosphatases (PTPs) to regulate cell function. SHP-2 is one such important PTP that also functions as an adaptor to promote downstream signaling pathway. Its role in Ang II signaling remains to be clarified. 相似文献16.
You-Lan Yang Hsin-Te Hsu Kuo-Hsien Wang Cheng-Ying Han Chien-Ming Chen Chi-Ming Chen Wun-Chang Ko 《Journal of biomedical science》2011,18(1):84
Background
Hesperetin was reported to selectively inhibit phosphodiesterase 4 (PDE4). While hesperetin-7,3'-O-dimethylether (HDME) is a synthetic liposoluble hesperetin. Therefore, we were interested in investigating its selectivity on PDE4 and binding ability on high-affinity rolipram-binding sites (HARBs) in vitro, and its effects on ovalbumin-induced airway hyperresponsiveness in vivo, and clarifying its potential for treating asthma and chronic obstructive pulmonary disease (COPD). 相似文献17.
18.
Masaki Uchida Xiong Wei Li Peter Mertens H. Oya Alpar 《Biochimica et Biophysica Acta (BBA)/General Subjects》2009
Background
Recently, particle bombardment has become increasingly popular as a transfection method, because of a reduced dependency on target cell characteristics. In this study, we evaluated in vitro gene transfer by particle bombardment.Methods
gWIZ luciferase and gWIZ green fluorescent protein (GFP) plasmids were used as reporter genes. Mammalian cell lines HEK 293, MCF7 and NIH/3T3 were used in the transfection experiments. Transfection was performed by bombardment of the cells with gene-coated gold particles using the Helios Gene Gun. The technology was assessed by analyzing gene expression and cell damage. Cell damage was evaluated by MTT assay.Results
This technology resulted in efficient in vitro transfection, even in the cells which are difficult to transfect. The gene expression was dependent on the gene gun's helium pressure, the sizes of the gold particles, the amount of the particles and DNA loading, while cell viability was mostly dependent on helium pressure and amount of the gold particles.Conclusions
This technology was useful to transfection of cells. Optimal transfection conditions were determined to be between 75 and 100 psi of helium pressure, 1.0 to 1.6 μm gold particle size and 0.5 mg of gold particle amount with a loading ratio of 4 μg DNA/mg gold particles.General significance
These findings will be useful in the design of gene gun device, and bring further improvements to the in vitro and in vivo transfection studies including gene therapy and vaccination. 相似文献19.
Mark T Albrecht Wei Wang Olga Shamova Robert I Lehrer Neal L Schiller 《Respiratory research》2002,3(1):18
Background
Pseudomonas aeruginosa and Burkholderia cepacia infections of cystic fibrosis patients' lungs are often resistant to conventional antibiotic therapy. Protegrins are antimicrobial peptides with potent activity against many bacteria, including P. aeruginosa. The present study evaluates the correlation between protegrin-1 (PG-1) sensitivity/resistance and protegrin binding in P. aeruginosa and B. cepacia. 相似文献20.