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1.
High temperature stress adversely affects wheat growth in many important production regions, but the mode of injury is unclear. Wheat ( Triticum aestivum L. cv. Newton) was grown under controlled conditions to determine the relative magnitude and sequences of responses of source and sink processes to high temperature stress during grain development. Regimes of 25°C day/15°C night, 30°C day/20°C night, and 35°C day/25°C night from 5 days after anthesis to maturity differentially affected source and sink processes. High temperatures accelerated the normal decline in viable leaf blade area and photosynthetic activities per unit leaf area. Electron transport, as measured by Hill reaction activity, declined earlier and faster than other photosynthetic processes at the optimum temperature of 25/15 °C and at elevated temperatures. Changes in RUBP carboxylase activities were similar in direction but smaller in magnitude than changes in photosynthesic rate. Increased protease activity during senscence was markedly accentuated by high temperature stress. Specific protease activity increased 4-fold at 25/15 °C and 28-fold at 35/25 °C from 0 to 21 days after initiation of temperature treatments. Grain-filling rate decreased from the lowest to the highest temperature, but the change was smaller than the decrease in grain-filling duration at the same temperatures. We concluded that a major effect of high temperature is acceleration of senescence, including cessation of vegetative and reproductive growth, deterioration of photosynthetic activities, and degradation of proteinaceous constituents. 相似文献
2.
Mediation of high-temperature injury by roots and shoots during reproductive growth of wheat 总被引:3,自引:0,他引:3
Abstract Previous studies suggest that high temperature stress on wheat (Triticum aestivum L.) involves root processes and acceleration of monocarpic senescence. Physiological changes in wheat roots and shoots were investigated to elucidate their relationship to injury from elevated temperatures after anthesis. Plants were grown under uniform conditions until 10 d after anthesis, when shoot/root regimes of 25°C/25°C, 25°C/35°C, 35°C/25°C and 35°C/35°C were imposed. Growth and senescence of shoots and grain were influenced more by root temperatures than by shoot temperatures. High root temperatures increased activities of protease and RNasc enzymes, and loss of chlorophyll, protein and RNA from shoots, whereas low root temperatures had opposite effects. High root temperatures appeared to induce shoot senescence directly. High shoot temperatures probably disrupted root processes, including export of cytokinins, and induced high leaf protease activity, senescence and cessation of grain development. The authors concluded that responses of wheat to high temperatures, whether of roots or shoots, are manifested as acceleration of senescence and may be mediated by roots during grain development. 相似文献
3.
Sucrose and fructan metabolism were studied in wheat ( Triticuin aotiirum L. cv. Tribal 800) roots during a period at chilling temperature. Enzyme activities related to fructan and sucrose metabolism were measured. Sucrose-sucrose fructosyl transfer-ase (EC 2.4.1.99) activity increased more than 25-fold when plants were cooled to 4°C. Sucrose synthase (EC 2.4.1.13) and sucrose-phosphate synthase (EC 2.4.1.14) activities also increased, but low temperatures had no significant effect on invertaso (EC 3.2.1.26) or on fructan hydrolase (EC 3.2.1.26) activities. The accumulation pattern of fructan in roots was different to that in leaves. In roots chilling stimulated the synthesis of fructans of high degree of polymerization. 相似文献
4.
The chlorophyll and protein contents of the flag, second and third leaves gradually decreased during the reproductive development of rice (Oryza sativa L. cv. Rasi) and wheat (Triticum aestivum L. cv. Sonalika) plants, whereas proline accumulation increased up to the grain maturation stage and slightly decreased thereafter. In rice plant, the rate of decrease in chlorophyll and protein and increase in proline level were higher in the flag leaf than in the second leaf. It was opposite in wheat plant. The export of [32P]-phosphate from leaves to grains gradually increased reaching a maximal stage at the grain development stage, and then declined. The export of this radioisotope was greater in rice than in wheat. Removal of panicle at the anthesis and grainfilling stages delayed leaf senescence of rice plant, while in wheat the ponicle removal at any stage did not have a marked effect on delaying leaf senescence. The contents of chlorophyll and protein of glumes were higher in wheat than in rice. The variation of such source-sink relationship might be one of the possible reasons for the above effect on leaf senescence. 相似文献
5.
The temperature dependence of C3 photosynthesis may be altered by the growth environment. The effects of long-term growth in elevated CO2 on photosynthesis temperature response have been investigated in wheat ( Triticum aestivum L.) grown in controlled chambers with 370 or 700 μmol mol−1 CO2 from sowing through to anthesis. Gas exchange was measured in flag leaves at ear emergence, and the parameters of a biochemical photosynthesis model were determined along with their temperature responses. Elevated CO2 slightly decreased the CO2 compensation point and increased the rate of respiration in the light and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) Vcmax , although the latter effect was reversed at 15°C. With elevated CO2 , Jmax decreased in the 15–25°C temperature range and increased at 30 and 35°C. The temperature response (activation energy) of Vcmax and Jmax increased with growth in elevated CO2 . CO2 enrichment decreased the ribulose 1,5-bisphosphate (RuBP)-limited photosynthesis rates at lower temperatures and increased Rubisco- and RuBP-limited rates at higher temperatures. The results show that the photosynthesis temperature response is enhanced by growth in elevated CO2 . We conclude that if temperature acclimation and factors such as nutrients or water availability do not modify or negate this enhancement, the effects of future increases in air CO2 on photosynthetic electron transport and Rubisco kinetics may improve the photosynthetic response of wheat to global warming. 相似文献
6.
Leaf senescence and grain filling affected by post-anthesis high temperatures in two different wheat cultivars 总被引:5,自引:0,他引:5
High temperature is a major factor affecting grain yield and plant senescence in wheat growing regions of central and east
China. In this study, two different wheat cultivars, Yangmai 9 with low-grain protein concentration and Xuzhou 26 with high-grain
protein concentration, were exposed to different temperature regimes in growth chambers during grain filling. Four day/night
temperature regimes of 34°C/22°C, 32°C/24°C, 26°C/14°C, and 24°C/16°C were established to obtain two daily temperatures of
28 and 20°C, and two diurnal day/night temperature differences of 12 and 8°C. Concentration of a lipid peroxidation product
malondialdehyde (MDA), activities of the antioxidants superoxide dismutase (SOD) and catalase (CAT), chlorophyll concentration
(SPAD) in flag leaves and kernel weight were determined. Results show that activities of SOD and CAT in leaves increased markedly
on 14 days after anthesis (DAA) for the high-temperature treatment (34°C/22°C) and then declined. As a result, MDA concentration
in leaves increased significantly under high temperature (34°C/22°C and 32°C/24°C). Compared with optimum temperature treatment,
high temperature reduced the concentration of soluble protein and SPAD values in flag leaves. Grain-filling rate increased
slightly initially, but decreased significantly during late grain filling under high temperature. As a result, final grain
weight was reduced markedly under high temperature. Decreases in the activities of SOD and CAT and increases in MDA concentration
in leaves were more pronounced with a 12°C of day/night temperature difference when under high temperatures. Kernel weight
was higher under 12°C of day/night temperature difference under optimum temperatures (24°C/16°C and 26°C/14°C). The responses
to high-temperature regimes appeared to differ between the two wheat cultivars with different grain protein concentrations.
It is concluded that a larger diurnal temperature difference hastened the senescence of flag leaves under high-temperature
conditions, but retarded senescence under optimum temperature treatments of 26°C/14°C and 24°C/16°C. 相似文献
7.
Some plants have the ability to maintain similar respiratory rates (measured at the growth temperature), even when grown at different temperatures, a phenomenon referred to as respiratory homeostasis. The underlying mechanisms and ecological importance of this respiratory homeostasis are not understood. In order to understand this, root respiration and plant growth were investigated in two wheat cultivars (Triticum aestivum L. cv. Stiletto and cv. Patterson) with a high degree of homeostasis, and in one wheat cultivar (T. aestivum L. cv. Brookton) and one rice cultivar (Oryza sativa L. cv. Amaroo) with a low degree of homeostasis. The degree of homeostasis (H) is defined as a quantitative value, which occurs between 0 (no acclimation) and 1 (full acclimation). These plants were grown hydroponically at constant 15 or 25 °C. A good correlation was observed between the rate of root respiration and the relative growth rates (RGR) of whole plant, shoot or root. The plants with high H showed a tendency to maintain their RGR, irrespective of growth temperature, whereas the plants with low H grown at 15 °C showed lower RGR than those grown at 25 °C. Among several parameters of growth analysis, variation in net assimilation rate per shoot mass (NARm) appeared to be responsible for the variation in RGR and rates of root respiration in the four cultivars. The plants with high H maintained their NARm at low growth temperature, but the plants with low H grown at 15 °C showed lower NARm than those grown at 25 °C. It is concluded that respiratory homeostasis in roots would help to maintain growth rate at low temperature due to a smaller decrease in net carbon gain at low temperature. Alternatively, growth rate per se may control the demand of respiratory ATP, root respiration rates and sink demands of photosynthesis. The contribution of nitrogen uptake to total respiratory costs was also estimated, and the effects of a nitrogen leak out of the roots and the efficiency of respiration on those costs are discussed. 相似文献
8.
Håkan Strid 《Physiologia plantarum》1996,97(1):5-12
Manifestations of aluminium (Al) toxicity in two cultivars of wheat ( Triticum aestivum L. cvs Kadett [relatively Al-resistant] and WW 20299 [relatively Al-sensitive]) were investigated at two root zone temperatures (RZT) that may occur in the field. The plants were grown for 9 days at 10 or 25°C RZT. Mineral nutrients other than CaSO4 were supplied daily in exponentially increasing amounts to meet the demand of the plants. Al was added as Al2 (SO4 )3 at the beginning of the culture period at concentrations ranging from 0 to 100 μ M . pH was kept constant at 4.1. Experimental data were analysed for interactions between Al and RZT on a fresh weight basis by the nonlinear Weibull function. Cultivar Kadett, when grown at 25°C RZT, was more resistant to Al than when grown at 10°C RZT. Cultivar WW 20299 was equally sensitive to Al at 10 and 25°C RZT but generally more sensitive to Al than cv. Kadett. It is suggested that cv. Kadett, in contrast to cv. WW 20299, possesses a mechanism for Al resistance that is less effective at 10°C than at 25°C RZT and therefore may be metabolically dependent. In roots, the concentrations of K, P, Mg and Ca were not negatively affected by Al or by RZT. In shoots of both cultivars the concentrations of Ca and Mg became comparatively low when the plants were treated with Al or at low RZT, the effect being larger for Ca than for Mg. At 10°C RZT under Al stress, the Ca concentrations in shoots approached the critical concentration where growth may be inhibited. As no Al was detected in the shoots, it is suggested that Al in the roots inhibits shoot growth by reducing transport of Ca from roots to shoots. 相似文献
9.
Wheat catalase expressed in transgenic rice can improve tolerance against low temperature stress 总被引:2,自引:0,他引:2
Takeshi Matsumura Noriko Tabayashi Yasuyo Kamagata Chihiro Souma Haruo Saruyama 《Physiologia plantarum》2002,116(3):317-327
We examined whether the expression of wheat catalase (EC 1.11.1.6) cDNA in transgenic rice ( Oryza sativa L.) could enhance tolerance against low temperature injury. Transgenic rice plants expressing wheat CAT protein showed an increase of activities in leaves at 25°C, 2- to 5-fold that in non-transgenic rice. At 5°C, catalase activities were about 4–15 times higher than those in non-transgenic rice were. A comparison of damage observed in leaves as they withered due to chilling at 5°C showed that transgenic rice displayed an increased capability to resist low temperature stress. The exposure of these plants to low temperature at 5°C for 8 days resulted in decreased catalase activities in leaves at 25°C, but the transgenic plants indicated 4 times higher residual catalase activities than those of non-transgenic ones. The concentration of H2 O2 in leaves was kept lower in transgenic rice than that of the control plants during the 8 days chilling. These results suggest that the improved tolerance against low temperature stress in genetically engineered rice plants be attributed to the effective detoxification of H2 O2 by the enhanced catalase activities. 相似文献
10.
Root growth, development and frost resistance were examined in winter rye ( Secale cereale L. cv. Puma) plants grown under 6 combinations of temperature and photoperiod (20/16°C or 5/3°C, day/night; 8, 16- or 24-h days). Overall root system growth is influenced by the interaction of temperature and photoperiod. Maximum shoot growth occurs at a 24-h photoperiod in 20°C plants and at a 16-h photoperiod in 5°C plants, and is correlated in both treatments with a high root:shoot ratio. Frost resistance of rye roots is affected by short photoperiods in 2 ways. First, short photoperiod and low temperature delay production of new adventitious roots so that newly developing roots are not exposed to freezing temperatures. Second, short photoperiod alone can induce several degrees of frost tolerance in existing roots during the lag phase of growth. Low temperature alone does not decrease the rate of dry weight accumulation in rye root systems, but cold temperature does retard developmental processes within the roots. Rye roots grown at 5°C develop first order lateral roots, differentiate metaxylem vessels and suberize endodermal cell walls more slowly than roots grown at 20°C. 相似文献
11.
CO2 , light and temperature influence senescence and protein degradation in wheat leaf segments 总被引:1,自引:0,他引:1
Effects of environmental conditions influencing photosynthesis and photorespiration on senescence and net protein degradation were investigated in segments from the first leaf of young wheat ( Triticum aestivum L. cv. Arina) plants. The segments were floated on H2 O at 25, 30 or 35°C in continuous light (PAR: 50 or 150 µmol m−2 s−1 ) in ambient air and in CO2 ‐depleted air. Stromal enzymes, including phosphoglycolate phosphatase, glutamine synthetase, ferredoxin‐dependent glutamate synthase, phosphoribulokinase, and the peroxisomal enzyme, glycolate oxidase, were detected by SDS‐PAGE followed by immunoblotting with specific antibodies. In general, the net degradation of proteins and chlorophylls was delayed in CO2 ‐depleted air. However, little effect of CO2 on protein degradation was observed at 25°C under the lower level of irradiance. The senescence retardation by the removal of CO2 was most pronounced at 30°C and at the higher irradiance. The stromal enzymes declined in a coordinated manner. Immunoreactive fragments from the degraded polypeptides were in most cases not detectable. However, an insolubilized fragment of glycolate oxidase accumulated in vivo, especially at 25°C in the presence of CO2 . Detection of this fragment was minimal after incubation at 30°C and completely absent on blots from segments kept at 35°C. In CO2 ‐depleted air, the fragment was only weakly detectable after incubation at 25°C. The results from these investigations indicate that environmental conditions that influence photosynthesis may interfere with senescence and protein catabolism in wheat leaves. 相似文献
12.
Some assumptions concerning development in wheat (Triticum aestivum, L.) were examined. These are that (i) the rate of development towards anthesis increases linearly with temperature, (ii) the base temperature is 0°C, (iii) the optimum temperature is above the range at which wheat is normally grown, (iv) base and optimum temperatures do not change with development, and (v) the relationships for different cultivars are similar. We tested these assumptions in studies using a naturally lit phytotron with four cultivars and six temperature regimes between 10 and 25°C. Seedlings were vernalized for 50 d and then grown under a photoperiod of 18 h to avoid confounding the responses to vernalization and photoperiod with those to temperature. In cultivars Sunset and Rosella, the rate of development for the full period to anthesis increased linearly between base and optimum temperatures. However, in cultivars Condor and Cappelle Desprez, a linear fit was not statistically acceptable. For these cultivars, the rate of development towards anthesis increased rapidly with increase in temperature from 10 to 19°C, but temperatures higher than 19°C had little or no fürther accelerating effect. When a linear relationship was fitted by ignoring data for temperatures above 19 7deg;C, base temperatures calculated for the full period to anthesis were c. 5.5, 5.5,4.0 and 2.5°C for Sunset, Condor, Rosella and Cappelle Desprez, respectively (i.e. an average value of c. 4 7deg;C). The full period to anthesis was subdivided into three phases for fürther analysis. These were (i) from the beginning of the experiment to terminal spikelet initiation, (ii) from terminal spikelet initiation to heading, and (iii) from heading to anthesis. When these sub-phases were analysed a linear relationship was found to be appropriate for all combinations of cultivar and developmental phase. However, both base and optimum temperatures calculated from the relationships increased as development progressed from (i) to (iii). Averaging across cultivars, base temperatures for the three phases were -1.9, %1.2 and %8.1°C, respectively, while optimum temperatures were <22, 25 and >25°C, respectively. Cultivars differed substantially in all these parameters. The progressive increase in optimum temperature with phasic development was apparently the main reason why linear fits for the three sub-phases became a curvilinear fit for the full phase to anthesis. 相似文献
13.
High temperatures during reproductive development alter kernel development and reduce yield and quality in wheat ( Triticum aestivum L.). Understanding how temperature alters kernel formation will help the development of genetic approaches to enhance heat tolerance in this cereal. A relationship between kernel development and postanthesis cytokinin accumulation is well documented, but the effect of temperature on this relationship is not known. This study quantified the effects of a postanthesis temperature treatment (7 d at 35/25 °C day/night) on kernel development and cytokinin accumulation in a soft white winter wheat (c.v. Stephens). Kernels from control plants maintained at 25/15 °C accumulated zeatin, dihydrozeatin and their corresponding 9-ribosides from 1 to 4 d postanthesis. Postanthesis kernel cytokinin accumulation was reduced by 50–80% by the temperature treatment. Kernel cytokinin content in control plants declined to baseline by 5 d postanthesis and remained at that level. Kernels from treated plants had a secondary peak of cytokinin accumulation 6–8 d after anthesis. This treatment significantly reduced kernel weight. The magnitude of the effect on kernel number was smaller than on kernel weight, but was statistically significant. Reduced kernel weight was accompanied by reduced cytokinin accumulation. Exogenous cytokinins did not mitigate the temperature effects on kernel weight, but at the highest concentrations, did reduce the effect on kernel number. 相似文献
14.
Abha Upadhyaya Tim D. Davis M. H. Larsen R. H. Walser N. Sankhla 《Physiologia plantarum》1990,79(1):78-84
Soybean [Glycine max(L.) Merr. cv. A2] seeds were germinated in 0 or 1 mg 11 (3.4 uM) uniconazole, after which seedling roots were excised and exposed to 22 or 48°C for 90 min. Prior to the temperature treatments there were few ultrastructural differences between uniconazole-treated seedling roots and the controls. Following exposure to 48°C, electron micrographs revealed near complete loss of normal ultrastructure in control epidermal root cells, whereas cellular integrity was maintained in treated roots, indicating that uniconazole conferred tolerance to high temperature. Total electrolyte, sugar and K+ leakage were all greater from control roots than treated roots during exposure to 48°C. Proline content in the roots was unaffected by uniconazole at 22°C but was 25–30% greater in treated tissue than in controls following exposure to 48°C. Malondialdehyde content was unaffected by uniconazole at 22°C but was nearly 20% less in treated tissue than in controls following high temperature exposure. This indicates that uniconazole decreased high-temperature-induced lipid peroxidation. Uniconazole elevated several antiox-idant systems in the roots, including water-soluble sulfhydryl concentration and catalase, peroxidase and superoxide dismutase activities. These findings are consistent with the hypothesis that uniconazole-induced stress tolerance is due, at least in part, to enhanced antioxidant activity which reduces stress-related oxidative damage to cell membranes. 相似文献
15.
High temperature (45°C) inhibits seed germinition and seedling sunflower ( Helianthus annuus L. cv. Mirasol). Treatment of imbibed seeds at 45°C for more than 48 h induces a secondary dormancy, which is associated with progressive decrease of germination ability at optimal temperature (25°C) as well as with abnormal seedling growth. Ethylene (55μl l−1 ) and 2-chloroethylphosphonic acid (ethephon) (2.5 m M ) improve germination of thermodormant seeds at 25°C. but the abnormal growth of the seedlings remains. O2 -enriched atmosphere and dry storage improve germination and normal seedling growth. The induction of thermodormancy in sunflower seeds seems associated with loss of their ability to convert 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene. Possible effects of high temperature on membranes and ethylene forming enzyme (EFE) are discussed. 相似文献
16.
Temperature modulates the cell wall mechanical properties of rice coleoptiles by altering the molecular mass of hemicellulosic polysaccharides 总被引:1,自引:0,他引:1
The present study was conducted to investigate the mechanism inducing the difference in the cell wall extensibility of rice ( Oryza sativa L. cv. Koshihikari) coleoptiles grown under various temperature (10–50°C) conditions. The growth rate and the cell wall extensibility of rice coleoptiles exhibited the maximum value at 30–40°C, and became smaller as the growth temperature rose or dropped from this temperature range. The amounts of cell wall polysaccharides per unit length of coleoptile increased in coleoptiles grown at 40°C, but not at other temperature conditions. On the other hand, the molecular size of hemicellulosic polysaccharides was small at temperatures where the cell wall extensibility was high (30–40°C). The autolytic activities of cell walls obtained from coleoptiles grown at 30 and 40°C were substantially higher than those grown at 10, 20 and 50°C. Furthermore, the activities of (1→3),(1→4)- β -glucanases extracted from coleoptile cell walls showed a similar tendency. When oat (1→3),(1→4)- β -glucans with high molecular mass were incubated with the cell wall enzyme preparations from coleoptiles grown at various temperature conditions, the extensive molecular mass downshifts were brought about only by the cell wall enzymes obtained from coleoptiles grown at 30–40°C. There were close correlations between the cell wall extensibility and the molecular mass of hemicellulosic polysaccharides or the activity of β -glucanases. These results suggest that the environmental temperature regulates the cell wall extensibility of rice coleoptiles by modifying mainly the molecular mass of hemicellulosic polysaccharides. Modulation of the activity of β -glucanases under various temperature conditions may be involved in the alteration of the molecular size of hemicellulosic polysaccharides. 相似文献
17.
Interaction of drought and high temperature on photosynthesis and grain-filling of wheat 总被引:32,自引:1,他引:31
Drought and high temperature often occur simultaneously, but their effects on crops are usually investigated individually. Our objective was to compare effects of drought, high temperature, and their interactions on photosynthesis and grain-growth of wheat (Triticum aestivum L.). Plants (cv. Len) were grown uniformly in well-watered soil at 25/20 ± 2 °C day/night until anthesis, when they were subjected to regimes of no drought (soil at field capacity) and drought (plant water potential of –.0 to –2.4 MPa) at 15/10, 25/20, and 35/30 °C in controlled environments until physiological maturity. Drought decreased photosynthesis, stomatal conductance, viable leaf area, shoot and grain mass, and weight and soluble sugar content of kernels but increased plant water-use efficiency. High temperature hastened the decline in photosynthesis and leaf area, decreased shoot and grain mass as well as weight and sugar content of kernels, and reduced water-use efficiency. Interactions between the two stresses were pronounced, and consequences of drought on all physiological parameters were more severe at high temperature than low temperature. The synergistic interactions indicated that productivity of wheat is reduced considerably more by the combined stresses than by either stress alone, and that much of the effect is on photosynthetic processes. 相似文献
18.
Microsclerotium formation by six isolates of Verticillium dahliae was studied at different temperatures both in vitro and in Arabidopsis thaliana . In vitro mycelial growth was optimal at 25°C, but microsclerotium formation was greatest at 20°C (two isolates) or 15–20°C (one isolate). Seedlings of A. thaliana were root-dipped in a conidial suspension, planted, and either placed at 5, 10, 15, or 25°C, or left at 20°C until the onset of senescence, after which some of the plants were placed at 5, 10, 15, or 25°C. The amount of microsclerotia per unit of shoot weight was assessed in relation to isolate and temperature. The optimal temperature for production of microsclerotia was 15–25°C. Two isolates each produced about 10 times more microsclerotia than each of the other four isolates. For these isolates, high R 2 adj. -values of 0.77 and 0.66 were obtained, with temperature and its square as highly significant (P < 0.001) independent variables. R 2 adj. -values for the other isolates varied between 0.28 and 0.39. Moving plants to different temperatures at the onset of senescence led to microsclerotial densities that were intermediate between densities on plants that had grown at constantly 20°C and plants grown at other temperatures. This suggests that vascular colonization rate and rate of microsclerotium formation are similarly affected by temperature. The senescence rate of plants appeared unimportant except for plants grown at 25°C, which showed the highest amounts of microsclerotia per unit of plant weight in the most rapidly senescing plants. 相似文献
19.
Influence of temperature on root development and phosphate influx in winter wheat grown at different P levels 总被引:1,自引:0,他引:1
Root development was studied in winter wheat ( Triticum aestivum L. cv Starke II) grown at 5,10, 15 and 20°C in nutrient solutions with phosphate concentrations of 10, 100 or 1000 μM . The plants were grown for 38 days (5 and 10°C), 19 days (15°C) or 14 days (20°C). At the end of the cultivation period the phosphate influx in the roots was determined with 32 P-phosphate. Root development (lateral and seminal roof length and number) was monitored throughout the cultivation period on the same individuals by repeated (approximately every second day) photocopying of the roots for measurements with digitizer and appropriate software. The 5°C treatment yielded no laterals, and the seminals were only slightly affected by the different phosphate treatments. The 10 μM phosphate treatment gave high root:shoot dry weight ratio, high average lateral root length and high specific root length [m root (g root fresh weight)-1 ]. The 1000 μM phosphate treatment yielded the highest number of laterals per m seminal root, and usually also the highest absolute numbers. Phosphate influx decreased with increased P status of the roots. It is argued that phosphate influx is dependent on factors such as P status, root geometry and relative root extension rate. 相似文献
20.
Effects of putrescine (Put) on responses of wheat (Triticum aestivum) seedlings or detached tillers at mid-milky stage to high temperature (HT) stress were investigated. The heat tolerant cv. PBW 343 exhibited higher content of antioxidants and activities of antioxidative enzymes, while lower content of lipid peroxides as compared to the heat-sensitive cv. HD 2329. HT elevated peroxidase (POX) and superoxide dismutase (SOD) activities, while diamine oxidase (DAO) and polyamine oxidase (PAO) activities were reduced in roots, shoots and developing grains. Application of Put under HT further enhanced POX and SOD activities along with increased content of ascorbate and tocophereol in grains. Invariably POX and SOD revealed higher activities in roots while CAT, DAO and PAO activities were higher in shoots. The content of lipid peroxides was increased in roots and shoots of HT stressed seedlings but less in Put-treated cv. PBW 343. 相似文献