Remote photoplethysmography with consumer smartphone reveals temporal differences between glabrous and nonglabrous skin: Pilot in vivo study

Photoplethysmography (PPG) is a noninvasive optical technology, with applications including vital sign extraction and patient monitoring. The PPG acquisition skin type may be of importance. Skin is either nonglabrous (~90%) or glabrous (~10%). Clinical PPG collection is typically from glabrous (fingerpad), while proliferating wearables collecting PPG, which may perform critical functions like arrythmia detection, often acquire from atypical sites. Glabrous skin has significant differences from nonglabrous, including microcirculation, yet comparisons between their PPG signals have not been well reported. Using a smartphone-based remote/contactless PPG, a pilot dataset was collected from the hands (palmar/dorsal) of five healthy volunteers. The data shows statistically significant lead time (52 ± 36 ms) of glabrous over nonglabrous. Further, a trend of glabrous amplitude increase over nonglabrous (31%) was found. Although our study has a small number of participants, these results further the characterization of PPG skin differences, and can be used to inform development of PPG-based devices.     

Grating orientation as a measure of tactile spatial acuity

Recent studies have used grating orientation as a measure of tactile spatial acuity on the fingerpad. In this task subjects identify the orientation of a grooved surface presented in either the proximal-distal or lateral-medial orientation. Other recent results have suggested that there might be a substantial anisotropy on the fingerpad related to spatial sensitivity. This anisotropy was revealed using a task in which subjects discriminated between a smooth and a grooved surface presented at different orientations on the fingerpad. The anisotropy was substantial enough that it might permit subjects to discriminate grating orientation on the basis of intensive rather than spatial cues. The present study examined the possibility that anisotropy on the fingerpad might provide cues in a spatial acuity task. The ability of subjects to discriminate between a smooth and a grooved surface was measured under conditions that are typically used in grating orientation tasks. No evidence of anisotropy was found. Also, using a grating orientation task, separate estimates were made of sensitivity in the proximal-distal and lateral-medial orientations. Again no evidence of anisotropy was found. Consistent with changes in the density of innervation, grating orientation sensitivity was found to vary as a function of location on the fingerpad. The results support the view that grating orientation is a valid measure of spatial acuity reflecting underlying neural, spatial mechanisms.     

Body Site Variation of Heat Pain Sensitivity

Thirty-two healthy human subjects provided thresholds for the perception of slight and moderate heat pain. Four body sites were tested bilaterally: thenar eminence of the hand, plantar surface of the foot, dorsolateral forearm, and lateral calf. Thresholds for the glabrous skin of the hand and foot were significantly greater than thresholds for the hairy skin of the arm and leg, the average difference being 1.3°c. Laterality was not a statistically significant factor. Thresholds increased progressively over 2–4 weeks of repeated testing, resulting in values averaging 0.6°c higher in the later sessions. The difference between moderate and slight pain thresholds averaged 1.1°c, and was consistent across body sites and with repeated testing.

The threshold values were normally distributed across subjects. Considerable intersubject variability was observed for both slight and moderate pain thresholds, more so on glabrous than on hairy skin sites. In comparison, the distribution of right-left difference values was narrower, demonstrating less intrasubject versus intersubject variability.

The highly significant difference in thresholds between glabrous and hairy skin sites demonstrates the importance of skin type for heat pain sensitivity. In contrast, there was no significant difference in heat pain sensitivity between comparable sites on the upper versus lower extremities, or between left and right sides.     

Organization of Meissner corpuscles in the glabrous skin of monkey and cat

Reconstructing neural-population responses in the form of spatial event plots assumes that the receptors are organized in a dense linear array. We have found that this assumption is not valid by determining the spatial organization of Meissner corpuscles (MCs) in the glabrous skin of both cat and monkey. The tissue was excised from animals that had been cardiac perfused with 4% paraformaldehyde. One-micrometer plastic sections revealed that the morphology of these receptors is different in the two animal species. However, in both species, they reside in approximately the same place in the dermal pegs of the skin, between the epidermal ridges, and electrophysiologically they both respond to ramp-and-hold stimuli with a rapidly adapting firing pattern. Thus, in this study we will refer to the receptors of the cat as "Meissner-like". In monkey, MCs are located in the dermal papillae between the epidermal limiting and intermediate ridges, forming orderly rows, the contours of which follow the overlying fingerprint. Although the average density of MCs is 45/mm2, they are distributed along the dermal pegs in such a manner as to give rise to three significantly ( p < 0.017) different average distances between corpuscles. We note that "fingerprints" vary in topography across the hand and this is also reflected in the underlying MC arrays. In the cat, these "Meissner-like" receptors display no specific pattern and have a density much lower than in the monkey. Cat glabrous skin does not have "fingerprints". The results emphasize that the spatial organization of tactile receptors must be taken into account when interpreting reconstructed population responses.     

Restoration of sensation to the hand by a free neurovascular flap from the first web space of the foot.

A neurovascular free flap from the first web space of the foot was used successfully in two patients for replacement of glabrous skin of the hand and fingers. The potential advantages of this flap are that (1) it may be used to replace large defects of glabrous skin, (2) it provides a rich vascular supply to the periphery of the hand, and (3) the sensation achieved approaches the normal for the intact glabrous skin of the hand.     

Spatial features of vibrotactile masking effects on airpuff-elicited sensations in the human hand

It was recently shown that the cutaneous sensitivity to airpuffs is decreased by a low-frequency vibrotactile masker in the hairy skin, and by a low-frequency but especially by a high-frequency masker in the glabrous skin. In the current study, the spatial features of this masking effect were determined in four healthy human subjects, using a reaction time paradigm. The masking effect decreased monotonically with increasing interstimulus distance, and identically in longitudinal and transverse (i.e., lateral) directions in the palm or dorsal surface of the hand. The masking effect was stronger in the glabrous than in the hairy skin, especially in the fingers. In the glabrous skin, the spread of masking effect produced by a high-frequency masker was more extensive than that produced by a low-frequency masker. The mechanical spread of high-frequency vibration was less extensive than that of low-frequency vibration in the skin. In the glabrous skin, a masker applied to the tip of the finger produced a stronger masking effect on sensations in the base of the finger than when the masker was located at the base and the test stimulus was located at the tip. It is concluded that mechanical spread of vibration in the skin is of minor importance in explaining the masking effects. Different peripheral neural mechanisms underlie the airpuff-elicited sensations in the hairy and glabrous skin. The afferent inhibitory mechanisms are stronger for signals coming from the glabrous skin of the fingers than for signals coming from the hairy skin. Furthermore, the peripheral innervation density and size of the cortical representational areas may be of importance in determining the magnitude of the masking effect.     

Spatial Features of Vibrotactile Masking Effects on Airpuff-Elicited Sensations in the Human Hand

It was recently shown that the cutaneous sensitivity to airpuffs is decreased by a low-frequency vibrotactile masker in the hairy skin, and by a low-frequency but especially by a high-frequency masker in the glabrous skin. In the current study, the spatial features of this masking effect were determined in four healthy human subjects, using a reaction time paradigm. The masking effect decreased monotonically with increasing interstimulus distance, and identically in longitudinal and transverse (i.e., lateral) directions in the palm or dorsal surface of the hand. The masking effect was stronger in the glabrous than in the hairy skin, especially in the fingers. In the glabrous skin, the spread of masking effect produced by a high-frequency masker was more extensive than that produced by a low-frequency masker. The mechanical spread of high-frequency vibration was less extensive than that of low-frequency vibration in the skin. In the glabrous skin, a masker applied to the tip of the finger produced a stronger masking effect on sensations in the base of the finger than when the masker was located at the base and the test stimulus was located at the tip. It is concluded that mechanical spread of vibration in the skin is of minor importance in explaining the masking effects. Different peripheral neural mechanisms underlie the airpuff-elicited sensations in the hairy and glabrous skin. The afferent inhibitory mechanisms are stronger for signals coming from the glabrous skin of the fingers than for signals coming from the hairy skin. Furthermore, the peripheral innervation density and size of the cortical representational areas may be of importance in determining the magnitude of the masking effect.     

The effects of cross-modal manipulations of attention on the detection of vibrotactile stimuli in humans

Although it is well known that attention to a visual or auditory stimulus can enhance its perception, less is known concerning the effects of attention on the perception of natural tactile stimuli. The present study was conducted to examine the magnitude of the effect of cross-modal manipulations of attention in human subjects on the detection of weak, low-frequency vibrotactile stimuli delivered to the glabrous skin of the finger pad of the right index finger via an Optacon. Three suprathreshold vibrotactile arrays (40 Hz), varying in the number of activated pegs and hence the area of skin stimulated, were used. Subjects were trained to detect the occurrence of vibrotactile or visual stimuli and to respond by pressing a foot pedal as quickly as possible thereafter. Two instructional lights were used to cue the subjects as to which stimulus modality they should attend, in three experimental conditions. In the first cue condition, the forthcoming stimulus modality was indicated by the illumination of its associated light. In the second cue condition, both instructional lights were illuminated, and the subjects were asked to divide their attention equally between the two modalities. In the third cue condition, the stimulus modality was falsely indicated by the illumination of the cue not associated with the stimulus to be presented. Reaction times (RTs) were calculated for each trial. For each modality, tactile and visual, the RTs varied significantly with the cue condition, with the mean RT changing in a graded manner across the experimental conditions (being shortest for the correctly cued condition, intermediate for the neutrally cued condition, and longest for the incorrectly cued condition.(ABSTRACT TRUNCATED AT 250 WORDS)     

Effects of muscle metaboreceptor stimulation on cutaneous blood flow from glabrous and nonglabrous skin in mildly heated humans.

Given differences in sympathetic innervation to glabrous and nonglabrous skin, we tested the hypothesis that muscle metaboreceptor regulation of cutaneous vascular conductance (CVC) differs between these skin regions. Subjects (n = 21) performed isometric handgrip exercise (IHG; 50% maximal voluntary contraction for 60 s), followed by 2 min of postexercise ischemia. Throughout IHG and postexercise ischemia, CVC was measured from glabrous (palm) and nonglabrous (forearm and chest) regions contralateral to the exercising arm. These procedures were conducted after the subjects had been exposed to an ambient temperature of 35 degrees C and a relative humidity of 50% for 60 min. These thermal conditions were intended to cause slight increases in cutaneous blood flow via sympathetic withdrawal. Esophageal, sublingual, and mean skin temperatures did not change markedly during IHG or postexercise ischemia. During IHG, forearm CVC did not change, chest CVC increased slightly, and palm CVC decreased substantially (from 100 to 34.8 +/- 3.5%; P = 0.001). During muscle metaboreceptor stimulation due to postexercise ischemia, CVC from nonglabrous regions returned to preexercise baselines, whereas CVC at the palm remained below preexercise baseline (68.2 +/- 4.2%; P = 0.001 relative to preexercise baseline). These results indicate that in mildly heated humans muscle metaboreflex stimulation is capable of modulating CVC in glabrous, but not in nonglabrous, skin.     

Responses of cutaneous mechanoreceptors within fingerpad to stimulus information for tactile softness sensation of materials

Softness sensation is one of primitive tactile textures. While the psychophysical characteristics of softness sensation have been thoroughly studied, it is lack of a deep understanding of the underlying neuromechanical principles. On the stimulus–response processes of human fingerpad touching fabrics and the physiological properties of slowly adapting type I (SAIs) cutaneous mechanoreceptors within fingerpad, a fabric-skin-receptor coupling model was built and validated. By the fabric-skin-receptor model a series of numerical experiments was conducted, and how the evoked neural responses of cutaneous mechanoreceptors change with the composite compliance of both fingerpad skin and the materials in contact was investigated. The results indicated that the evoked neural responses of populations of cutaneous mechanoreceptors by the physical stimulus from fabrics were nearly proportional to the perceived softness magnitude, and nonlinearly increased and then decreased with the effective elastic modulus of fabrics or the relative elastic modulus of fabrics to soft tissues within fingerpad, where the nonlinear inflection point depended on the touching force level. Therefore, it concluded that the tactile judgment of the physical information for softness sensation of objects was an encoding of neural responses of populations of SAIs cutaneous mechanoreceptors, and the physical information depended on the mechanical interaction of fingerpad and objects in contact.     

Muscle activation during maximal voluntary eccentric and concentric knee extension

The aim of this investigation was to study the relationships among movement velocity, torque output and electromyographic (EMG) activity of the knee extensor muscles under eccentric and concentric loading. Fourteen male subjects performed maximal voluntary eccentric and concentric constant-velocity knee extensions at 45, 90, 180 and 360 degrees.s-1. Myoelectric signals were recorded, using surface electrodes, from the vastus medialis, vastus lateralis and rectus femoris muscles. For comparison, torque and full-wave rectified EMG signals were amplitude-averaged through the central half (30 degrees-70 degrees) of the range of motion. For each test velocity, eccentric torque was greater than concentric torque (range of mean differences: 20%-146%, P less than 0.05). In contrast, EMG activity for all muscles was lower under eccentric loading than velocity-matched concentric loading (7%-31%, P less than 0.05). Neither torque output nor EMG activity for the three muscles changed across eccentric test velocities (P greater than 0.05). While concentric torque increased with decreasing velocity, EMG activity for all muscles decreased with decreasing velocity (P less than 0.05). These data suggest that under certain high-tension loading conditions (especially during eccentric muscle actions), the neural drive to the agonist muscles was reduced, despite maximal voluntary effort. This may protect the musculoskeletal system from an injury that could result if the muscle was to become fully activated under these conditions.     

Dynamic autoregulation of cutaneous circulation: differential control in glabrous versus nonglabrous skin

The purpose of this project was to test the hypothesis that, independent of neural control, glabrous and nonglabrous cutaneous vasculature is capable of autoregulating blood flow. In 10 subjects, spectral and transfer function analyses of arterial pressure and skin blood flow (laser-Doppler flowmetry) from glabrous (palm) and nonglabrous (forearm) regions were performed under three conditions: baseline, ganglionic blockade via intravenous trimethaphan administration, and trimethaphan plus oscillatory lower body negative pressure (LBNP; -5 to -10 mmHg) from 0.05 to 0.07 Hz. Oscillatory LBNP was applied to regenerate mean arterial pressure variability that was abolished by ganglionic blockade. Ganglionic blockade was verified by an absence of a heart rate response to a Valsalva maneuver. Spectral power and transfer function gain between blood pressure and skin blood flow were calculated in this oscillatory frequency range (0.05-0.07 Hz). Within this frequency range, ganglionic blockade significantly decreased spectral power of blood flow in both the forearm and palm, whereas regeneration of arterial blood pressure oscillations significantly increased spectral power of forearm blood flow but not palm blood flow. During oscillatory LBNP, transfer function gain between blood pressure and skin blood flow was significantly elevated at the forearm (0.28 +/- 0.03 to 0.53 +/- 0.02 flux units/mmHg; P < 0.05) but was reduced at the palm (4.7 +/- 0.5 to 1.2 +/- 0.1 flux units/mmHg; P < 0.05). These data show that independent of neural control of blood flow, glabrous skin has the ability to buffer blood pressure oscillations and demonstrates a degree of dynamic autoregulation. Conversely, these data suggest that nonglabrous skin has diminished dynamic autoregulatory capabilities.     

Thermogenic and psychogenic recruitment of human eccrine sweat glands: Variations between glabrous and non-glabrous skin surfaces

Human eccrine sweat-gland recruitment and secretion rates were investigated from the glabrous (volar) and non-glabrous hand surfaces during psychogenic (mental arithmetic) and thermogenic stimuli (mild hyperthermia). It was hypothesised that these treatments would activate glands from both skin surfaces, with the non-thermal stimulus increasing secretion rates primarily by recruiting more sweat glands. Ten healthy men participated in two seated, resting trials in temperate conditions (25–26 °C). Trials commenced under normothermic conditions during which the first psychogenic stress was applied. That was followed by passive heating (0.5 °C mean body temperature elevation) and thermal clamping, with a second cognitive challenge then applied. Sudomotor activity was evaluated from both hands, with colourimetry used to identify activated sweat glands, skin conductance to determine the onset of precursor sweating and ventilated sweat capsules to measure rates of discharged sweating. From glandular activation and sweat rate data, sweat-gland outputs were derived. These psychogenic and thermogenic stimuli activated sweat glands from both the glabrous and non-glabrous skin surfaces, with the former dominating at the glabrous skin and the latter at the non-glabrous surface. Indeed, those stimuli individually accounted for ~90% of the site-specific maximal number of activated sweat glands observed when both stimuli were simultaneously applied. During the normothermic psychological stimulation, sweating from the glabrous surface was elevated via a 185% increase in the number of activated glands within the first 60 s. The hypothetical mechanism for this response may involve the serial activation of additional eccrine sweat glands during the progressive evolution of psychogenic sweating.     

Is baldness a thermoregulatory adaptive process?

In 100 adult men the area of the face and neck where beard was growing was measured and compared to that of glabrous skin on the forehead and calvaria. In the population as a whole, forehead area was found to be proportional to bearded area. Forehead and calvaria sweat rate was measured on 10 baldheaded male subjects and compared with that of 10 hairy control subjects during mild hyperthermia. Bald skin was found to sweat more than twice as much as hairy skin. In the light of these results the hypothesis that baldness is a thermoregulatory adaptative process is proposed.     

Effects of head cooling on human sleep stages and body temperature

The purpose of this study was to confirm the effect of head cooling on human sleep stages and body temperature. Nine healthy male volunteers with a mean age of 25 +/- 3.77 years served as subjects. The experiments were carried out under three different sets of conditions: 26 degrees C, relative humidity (RH) 50% (26/50); 32 degrees C, RH 80% (32/80); and 32 degrees C RH 80% with the use of a cooling pillow (32/80 HC). The subjects slept from 2300 hours to 0700 hours with a cotton blanket, wearing short-sleeved pyjamas and shorts on a bed, which was covered with a sheet. Electroencephalograms, electro-ouclogram, and mental electromyelograms were recorded through the night. Rectal temperature (Tre) and skin temperature (Tsk) were measured continuously. Whole-body sweat and the tympanic temperature (Tty) were measured before and after sleep. Wakefulness significantly increased at 32/80 than at 26/50; however, no significant difference was observed between 32/80 HC and 26/50. Tre and mean Tsk were higher both at 32/80 and 32/80 HC than at 26/50. The whole-body sweat loss was significantly greater and Tty in the morning was higher at 32/80 than 32/80 HC and 26/50. These results suggest that head cooling during sleep may help to decrease the whole-body sweat rate during sleep under humid heat conditions.     

Functional Properties of Slowly Adapting Mechanoreceptors in Cat Footpad Skin

The functional properties of slowly adapting (SA) afferent fibers innervating cat footpad skin were examined. Measurements were taken of receptive field area; spontaneous activity (< 1 impulse/sec); the slope of the stimulus-response curve for steady indentations up to 2 mm in amplitude; variability of the interimpulse intervals, as measured by the coefficient of variation of time interval histograms; decay of the response to steady indentation; and sensitivity to sinusoidal vibration (most sensitive at 5-10 Hz). Where comparable tests were performed on glabrous and hairy skin SA fibers, the functional properties of those in glabrous skin more closely resembled SAI fibers than SAII fibers. Additional results from glabrous skin SA fibers suggest that it is distortion of the nerve endings rather than steady indentation or compression that leads to a brisk response. On the measures described above, there appeared to be only one functional class of SA fiber innervating the cat footpad skin.     

Ambient temperature affects glabrous skin vasculature and sweating responses to mental task in humans

We compared responses in heart rate (HR), mean blood pressure (MAP), sweating rate (SR), sweating expulsion (SwE), and skin vascular conductance (VC) to mental task among different ambient temperature (Ta) conditions, i.e., 12, 16, 20, and 24 degrees C. Seven subjects (27+/-5 yrs, 64+/-14 kg) underwent a 2-min color word conflict test (CWT) after 2 mins of baseline data acquisition following a 20-min resting period. All subjects wore long sleeve shirts and long pants. The skin blood flow was measured with a laser Doppler probe on the left index finger pulp to calculate skin VC, and the SR and sweating expulsion (SwE) were measured with a ventilated capsule on the left thenar. CWT significantly increased the HR and MAP, while there was no significant effect of Ta on the magnitudes of these responses. CWT significantly decreased the skin VC when the Ta was 24 degrees C, whereas it significantly increased the skin VC when the Ta was 12 or 16 degrees C. CWT significantly increased SR and SwE in all Ta conditions, and the SwE was greater in warmer conditions. These findings suggest that different ambient temperatures induce different responses in finger skin vasculature to mental task, implying the independent response of cutaneous vasomotor tone and sweat glands in glabrous skin to mental task.     

Localization of Merkel cells in the monkey skin: an anatomical model

The Merkel cell-neurite complex is considered to be one class of mechanoreceptors in the skin. Merkel cells are innervated by slowly adapting type I (SAI) tactile nerve fibers. In this paper, the detailed distribution of Merkel cells is studied by immunohistochemical labeling of the monkey (Macaca fascicularis) digital glabrous skin. Specific morphometric variables (density of intermediate epidermal ridges and Merkel cells, distance between skin surface and ridge tips and bases, maximum and average cell counts per ridge, distance between cells and ridges) were measured by a combination of light/fluorescence microscopy and computer-image analysis. The morphometric results were similar for each digit of the monkey's hand. Next, the anatomical data were used to form a three-dimensional reconstruction of the Merkel-cell distribution in the fingertip skin. A patch of the distal-pad surface was then computationally flattened to obtain the two-dimensional distribution of Merkel cells. Based on previous anatomical and physiological data, SAI fibers were simulated to innervate clusters of Merkel cells in the distal-pad surface. On average, 28 cells were innervated by a single fiber. The resulting anatomical model may be used to estimate the population response of SAI fibers by incorporating spike generation.     

On the ftight capabilities and distribution of the giant Miocene bird Argentavis magnificens (Teratornithidae)

Argentavis magnificens , the largest known flying bird, had a wingspan of over 6 m with a mass of 80 kg. Its enormous size suggests that it was not a powerful flapper. The wing shape is inferred as more like that of large extant birds that soar relatively slowly on thermals over land than of large pelagic birds that soar over water. Its high wing loading would have allowed it to fly in moderate to strong winds that must have been prohibitive for the largest known contemporary thermal soarers. The high wing loading would have been ill-suited to flight under poor thermal conditions, but it would have been useful in slope-soaring on uprising air current against hillsides. We propose that Argentavis had a large home range that included a nesting area in the mountains of western and northwestern Argentina, and a feeding area in the Pampas.     

Effects of airflow on body temperatures and sleep stages in a warm humid climate

Airflow is an effective way to increase heat loss—an ongoing process during sleep and wakefulness in daily life. However, it is unclear whether airflow stimulates cutaneous sensation and disturbs sleep or reduces the heat load and facilitates sleep. In this study, 17 male subjects wearing short pyjamas slept on a bed with a cotton blanket under two of the following conditions: (1) air temperature (Ta) 26°C, relative humidity (RH) 50%, and air velocity (V) 0.2 m s⁻¹; (2) Ta 32°C, RH 80%, V 1.7 m s⁻¹; (3) Ta 32°C; RH 80%, V 0.2 m s⁻¹ (hereafter referred to as 26/50, 32/80 with airflow, and 32/80 with still air, respectively). Electroencephalograms, electrooculograms, and mental electromyograms were obtained for all subjects. Rectal (Tre) and skin (Ts) temperatures were recorded continuously during the sleep session, and body-mass was measured before and after the sleep session. No significant differences were observed in the duration of sleep stages between subjects under the 26/50 and 32/80 with airflow conditions; however, the total duration of wakefulness decreased significantly in subjects under the 32/80 with airflow condition compared to that in subjects under the 32/80 with still air condition (P < 0.05). Tre, Tsk, Ts, and body-mass loss under the 32/80 with airflow condition were significantly higher compared to those under the 26/50 condition, and significantly lower than those under the 32/80 with still air condition (P < 0.05). An alleviated heat load due to increased airflow was considered to exist between the 32/80 with still air and the 26/50 conditions. Airflow reduces the duration of wakefulness by decreasing Tre, Tsk, Ts, and body-mass loss in a warm humid condition.