共查询到20条相似文献,搜索用时 31 毫秒
1.
Telomere length determined by the fluorescence in situ hybridisation distinguishes malignant and benign cells in cytological specimens
下载免费PDF全文
![点击此处可从《Cytopathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Y. Matsuda A. Suzuki S. Esaka Y. Hamashima M. Imaizumi M. Kinoshita H. Shirahata Y. Kiso H. Kojima M. Matsukawa Y. Fujii N. Ishikawa J. Aida K. Takubo T. Ishiwata M. Nishimura T. Arai 《Cytopathology》2018,29(3):262-266
Background
Telomeres are tandem repeats of TTAGGG at the end of eukaryotic chromosomes that play a key role in preventing chromosomal instability. The aim of the present study is to determine telomere length using fluorescence in situ hybridisation (FISH) on cytological specimens.Methods
Aspiration samples (n = 41) were smeared on glass slides and used for FISH.Results
Telomere signal intensity was significantly lower in positive cases (cases with malignancy, n = 25) as compared to negative cases (cases without malignancy, n = 16), and the same was observed for centromere intensity. The difference in DAPI intensity was not statistically significant. The ratio of telomere to centromere intensity did not show a significant difference between positive and negative cases. There was no statistical difference in the signal intensities of aspiration samples from ascites or pleural effusion (n = 23) and endoscopic ultrasound‐guided FNA samples from the pancreas (n = 18).Conclusions
The present study revealed that telomere length can be used as an indicator to distinguish malignant and benign cells in cytological specimens. This novel approach may help improve diagnosis for cancer patients. 相似文献2.
Ana M Enciu Mihnea I Nicolescu Catalin G Manole Dafin F Mure?anu Lauren?iu M Popescu Bogdan O Popescu 《BMC neurology》2011,11(1):75
Background
Neuroregeneration is a relatively recent concept that includes neurogenesis, neuroplasticity, and neurorestoration - implantation of viable cells as a therapeutical approach. 相似文献3.
Erin Henry Luca Santini Mark A. J. Huijbregts Ana Benítez-López 《Global Ecology and Biogeography》2023,32(2):267-280
Aim
Whether intraspecific spatial patterns in body size are generalizable across species remains contentious, as well as the mechanisms underlying these patterns. Here we test several hypotheses explaining within-species body size variation in terrestrial vertebrates including the heat balance, seasonality, resource availability and water conservation hypotheses for ectotherms, and the heat conservation, heat dissipation, starvation resistance and resource availability hypotheses for endotherms.Location
Global.Time period
1970–2016.Major taxa studied
Amphibians, reptiles, birds and mammals.Methods
We collected 235,905 body size records for 2,229 species (amphibians = 36; reptiles = 81; birds = 1,545; mammals = 567) and performed a phylogenetic meta-analysis of intraspecific correlations between body size and environmental variables. We further tested whether correlations differ between migratory and non-migratory bird and mammal species, and between thermoregulating and thermoconforming ectotherms.Results
For bird species, smaller intraspecific body size was associated with higher mean and maximum temperatures and lower resource seasonality. Size–environment relationships followed a similar pattern in resident and migratory birds, but the effect of resource availability on body size was slightly positive only for non-migratory birds. For mammals, we found that intraspecific body size was smaller with lower resource availability and seasonality, with this pattern being more evident in sedentary than migratory species. No clear size–environment relationships were found for reptiles and amphibians.Main conclusions
Within-species body size variation across endotherms is explained by disparate underlying mechanisms for birds and mammals. Heat conservation (Bergmann's rule) and heat dissipation are the dominant processes explaining biogeographic intraspecific body size variation in birds, whereas in mammals, body size clines are mostly explained by the starvation resistance and resource availability hypotheses. Our findings contribute to a better understanding of the mechanisms behind species adaptations to the environment across their geographic distributions. 相似文献4.
Nicole Philippi Dorothee Walter Rebekka Schlatter Karine Ferreira Michael Ederer Oliver Sawodny Jens Timmer Christoph Borner Thomas Dandekar 《BMC systems biology》2009,3(1):97-11
Background
The decision pro- or contra apoptosis is complex, involves a number of different inputs, and is central for the homeostasis of an individual cell as well as for the maintenance and regeneration of the complete organism. 相似文献5.
Background
Synesthesia is, as visual migraine aura, a common and fascinating perceptual phenomenon. Here we present a unique case with synesthesias exclusively during visual migraine auras. 相似文献6.
Brittany E. Harlow Michael D. Flythe Glen E. Aiken 《Journal of applied microbiology》2018,124(1):58-66
Aims
The objective was to determine the effect of the isoflavone biochanin A (BCA) on rumen cellulolytic bacteria and consequent fermentative activity.Methods and Results
When bovine microbial rumen cell suspensions (n = 3) were incubated (24 h, 39°C) with ground hay, cellulolytic bacteria proliferated, short‐chain fatty acids were produced and pH declined. BCA (30 μg ml?1) had no effect on the number of cellulolytic bacteria or pH, but increased acetate, propionate and total SCFA production. Addition of BCA improved total digestibility when cell suspensions (n = 3) were incubated (48 h, 39°C) with ground hay, Avicel, or filter paper. Fibrobacter succinogenes S85, Ruminococcus flavefaciens 8 and Ruminococcus albus 8 were directly inhibited by BCA. Synergistic antimicrobial activity was observed with BCA and heat killed cultures of cellulolytic bacteria, but the effects were species dependent.Conclusions
These results indicate that BCA improves fibre degradation by influencing cellulolytic bacteria competition and guild composition.Significance and Impact of the Study
BCA could serve as a feed additive to improve cellulosis when cattle are consuming high‐fibre diets. Future research is needed to evaluate the effect of BCA on fibre degradation and utilization in vivo. 相似文献7.
Background
Integrin-linked kinase (ILK) is a ubiquitously expressed protein kinase that has emerged as one of the points of convergence between integrin- and growth factor-signalling pathways. 相似文献8.
Background
The paca is a South American rodent with potential as a commercial food animal. We examined paca placenta as part of a wider effort to understand the reproductive biology of this species. 相似文献9.
Uberto Pozzoli Giorgia Menozzi Matteo Fumagalli Matteo Cereda Giacomo P Comi Rachele Cagliani Nereo Bresolin Manuela Sironi 《BMC evolutionary biology》2008,8(1):99
Background
Mammalian genomes consist of regions differing in GC content, referred to as isochores or GC-content domains. The scientific debate is still open as to whether such compositional heterogeneity is a selected or neutral trait. 相似文献10.
Phelipe O Favaron Anthony M Carter Carlos E Ambrósio Adriana C Morini Andrea M Mess Moacir F de Oliveira Maria A Miglino 《Reproductive biology and endocrinology : RB&E》2011,9(1):55
Background
Sigmodontinae, known as "New World rats and mice," is a large subfamily of Cricetidae for which we herein provide the first comprehensive investigation of the placenta. 相似文献11.
Jiin-Haur Chuang Hui-Ching Chuang Chao-Cheng Huang Chia-Ling Wu Yung-Ying Du Mei-Lang Kung Chih-Hao Chen San-Cher Chen Ming-Hong Tai 《Journal of biomedical science》2011,18(1):65
Background
Toll-like receptor-3 (TLR-3) is a critical component of innate immune system against dsRNA viruses and is expressed in the central nervous system. However, it remains unknown whether TLR3 may serve as a therapeutic target in human neuroblastoma (NB). 相似文献12.
Effect of menstrual cycle phase and hormonal treatments on evaluation of tubal patency in baboons
下载免费PDF全文
![点击此处可从《Journal of medical primatology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jeffrey T. Jensen Carol Hanna Emily Mishler Jeong Y. Lim Ov D. Slayden 《Journal of medical primatology》2018,47(1):40-45
Background
We evaluated whether menstrual cycle phase influences the assessment of tubal patency by hysterosalpingography (HSG) in baboons.Methods
Retrospective analysis of baseline tubal patency studies and serum estradiol (E2) and progesterone (P4) values obtained from female baboons used as models for development of non‐surgical permanent contraception in women. The main outcome measure was bilateral tubal patency (BTP) in relationship with estradiol level.Results
Female baboons (n = 110) underwent a single (n = 81), two (n = 26), or three (n = 3) HSG examinations. In 33/142 (23%) HSG examinations, one or both tubes showed functional occlusion (FO). The median E2 in studies with BTP (49 pg/mL) was significantly higher than in those studies with FO (32 pg/mL, P = .005). Among 18 animals with repeat examinations where serum E2 changed from <60 to ≥ 60 pg/mL, 13 results changed from FO to BTP (P = .0001). No sets showed a change from BTP to FO with an increase in estradiol.Conclusion
In baboons, functional occlusion of the fallopian tube is associated with low estradiol levels, supporting a role for estrogen‐mediated relaxation of the utero‐tubal junction. 相似文献13.
Background
The power of microarray analysis can be realized only if data is systematically archived and linked to biological annotations as well as analysis algorithms. 相似文献14.
Towards the systematic discovery of signal transduction networks using phosphorylation dynamics data
Haruna Imamura Nozomu Yachie Rintaro Saito Yasushi Ishihama Masaru Tomita 《BMC bioinformatics》2010,11(1):232
Background
Phosphorylation is a ubiquitous and fundamental regulatory mechanism that controls signal transduction in living cells. The number of identified phosphoproteins and their phosphosites is rapidly increasing as a result of recent mass spectrometry-based approaches. 相似文献15.
Brian T Smith Jason C Mussell Paul A Fleming Jeremy L Barth Demetri D Spyropoulos Marion A Cooley Christopher J Drake Scott W Argraves 《BMC developmental biology》2006,6(1):30-12
Background
Cubilin is a peripheral membrane protein that interacts with the integral membrane proteins megalin and amnionless to mediate ligand endocytosis by absorptive epithelia such as the extraembryonic visceral endoderm (VE). 相似文献16.
Kim JS Kroin JS Li X An HS Buvanendran A Yan D Tuman KJ van Wijnen AJ Chen D Im HJ 《Arthritis research & therapy》2011,13(5):R165
Introduction
Degeneration of the interverterbral disk is as a cause of low-back pain is increasing. To gain insight into relationships between biological processes, structural alterations and behavioral pain, we created an animal model in rats. 相似文献17.
BACTIBASE second release: a database and tool platform for bacteriocin characterization 总被引:2,自引:0,他引:2
Riadh Hammami Abdelmajid Zouhir Christophe Le Lay Jeannette Ben Hamida Ismail Fliss 《BMC microbiology》2010,10(1):22
Background
BACTIBASE is an integrated open-access database designed for the characterization of bacterial antimicrobial peptides, commonly known as bacteriocins. 相似文献18.
Background
Recent research show that polycystic ovary syndrome (PCOS) may have an association with low-grade chronic inflammation, IL-18 is considered as a strong risk marker of inflammation. 相似文献19.
20.
Pamela W Duncan Katherine J Sullivan Andrea L Behrman Stanley P Azen Samuel S Wu Stephen E Nadeau Bruce H Dobkin Dorian K Rose Julie K Tilson The LEAPS Investigative Team 《BMC neurology》2007,7(1):39