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将含有鸡传染性支气管炎病毒 S1 基因c D N A 的重组转移质粒p S X I V V I+ X3 S1 . Holte 和p S X I V V I+ X3/4 S1 . Holte 分别与粉纹夜蛾核型多角体病毒 Tn N P V S V I- G D N A( O C C- ,gal+ ) 共转染草地夜蛾( Sf9) 细胞,经空斑纯化得到重组病毒 Tn N P V( X3) S1 . Holte O C C+ 和 Tn N P V( X3/4) S1 . Holte O C C+ 。将重组毒株分别感染 Tn5 B1 细胞,并进行 S D S P A G E 与 Westernblot 检测。结果表明, Tn N P V( X3/4) S1 . Holte O C C+ 在感染的细胞中高效表达了 S1 蛋白, S D S P A G E 凝胶薄层色谱分析结果显示,感染病毒后72 h S1 蛋白的表达量占细胞内总蛋白量的35 .8 % ,而 Tn N P V( X3) S1 . Holte O C C+ 感染的细胞内检测不出 S1 蛋白。经分析认为这一差异主要来自 S1 基因翻译起始位点及其附近的周围环境。 相似文献
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表达马铃薯X病毒和Y病毒双价外壳蛋白基因马铃薯植株的抗病性 总被引:2,自引:0,他引:2
表达马铃薯X病毒(PVX)和马铃薯X病毒(PVY)双价外壳蛋白(CP)基因的马铃薯虎头和克新4号,用机械摩擦同时接种PVX和PVY后,通过症状观察,植株中PVX和PVY的ELISA检测结果表明,转基因头虎头和克新4号的多数株系的平均病毒含量均明显低于未转基因的对照植株,不同时期病毒测定结果表明,许多株系病毒积累缓慢,延迟发病,说明转PVX,PVY双价CP基因的马铃薯,对PVX和PVY复合侵染发生不 相似文献
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植物组织粗汁液中的番木瓜环斑病毒的ELISA检测技术 总被引:14,自引:0,他引:14
本研究建立和改进了检测番木瓜和西葫芦组织粗汁液里的番木瓜环斑病毒(PRV)的DAC-ELISA法和Dot-ELISA法。用不同的ELISA方法来检测不同寄主植物粗汁液里的PRV,其所用的合适的制备粗汁液的缓冲液是不同的。用DAC-ELISA法检测西葫芦粗汁液时,以0.5mol/L磷酸盐缓冲液(pH7.5,内含0.1mol/L乙二胺四乙酸二钠)为宜;而检测番木瓜粗汁液时,则还要加入0.25mol/L脲。用Dot-ELISA法检测时,在上述磷酸盐缓冲液中加入2%聚乙烯吡咯烷铜能提高对西葫芦粗汁液的检测效果。应用合适的制备粗汁液的缓冲液,DAC-ELISA法和Dot-ELISA法的灵敏度分别提高到1/4096和1/1024(稀释度)。本研究还表明,影响DAC-ELISA法的定过测定的主要因素是粗汁液的稀释度和包被液(0.05mol/L碳酸盐缓冲液,pH9.6)的用过。在较高粗汁液稀释度和包被液的用量相同时,粗汁液里的病毒含量与DAC-ELISA法的OD492nm值呈真实的线性关系。 相似文献
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常春藤阶段转变研究概况 总被引:3,自引:0,他引:3
常春藤阶段转变研究概况包劲松1李载龙1夏英武2(浙江农业大学,1.园艺系,2.核农所,杭州,310029)ASURVEYFORPHAESCHANGEINIVYBaoJing-song1LiZai-long1XiaYing-wu2(1.Departm... 相似文献
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兰科植物传粉生物学研究概述 总被引:6,自引:0,他引:6
兰科植物传粉生物学研究概述赵运林(湘潭师范学院,湘潭411201)ASURVEYONTHESTUDYOFPOLLINATIONBIOLOGYOFORCHIDACEOUSPLANTS¥ZhaoYun-lin(XiaxgtanTeacher'sColle... 相似文献
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人I型胶原基因第一内含子调节转录的研究 总被引:3,自引:0,他引:3
人I型胶原α1(I)链(COLIA1)基因内含子序列在不同细胞内有不同的转录调节活性,报道了含人COLIA1基因内含子I不同区段(+544~+855和+820~+1093)的重组质粒pSCEP-CAT和pSCIP-CAT的构建并转染人胚肌腱成纤维细胞和Tca8113舌癌细胞,地高辛标记抗CAT-ELISA检测结果显示:pSCEP-CAT在两种细胞均获表达;pSCIP-CAT在人成纤维细胞未表达,但 相似文献
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DETECTIONOFINFECTIOUSVIRUSANDVIRALRNAINTHEMYOCARDIUMOFMICEINFECTEDEXPERIMENTALLYWITHCOXSACKIEVIRUS’B3XiaomeiOuyang,HongyiZhan... 相似文献
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香蕉试管苗黄瓜花叶病毒检疫检验技术研究 总被引:3,自引:0,他引:3
建立了香蕉试管苗黄瓜花叶病毒DAC-ELISA检测技术。应用0.1mol/L磷酸盐缓冲液(pH7.2,内含1%NaDIECA)作为样品制备缓冲液,检测灵敏度可达到1/5120(稀释度)。两年来检测了1287个样品,香蕉试管苗生产繁殖材料的带毒率为2.8%。 相似文献
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Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis
It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis. 相似文献
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RICHARD E. NORRIS 《Botanical journal of the Linnean Society. Linnean Society of London》1991,106(1):1-40
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera. 相似文献
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JOAN VALUÈS MONTSERRAT TORRELL NÚRIA GARCIA JACAS 《Botanical journal of the Linnean Society. Linnean Society of London》2001,137(4):399-407
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted. 相似文献
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肝癌中HBV和HCV基因和抗原的分布及意义 总被引:1,自引:0,他引:1
采用原位分子杂交方法检测HCV
RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV
RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV
RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV
X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV
C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细 相似文献
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H.-R. GREGORIUS 《Biological journal of the Linnean Society. Linnean Society of London》1984,23(2-3):157-165
For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment. 相似文献