Peptidasen

Summary Using fresh frozen (with and without semipermeable membranes), freeze-dried or sections from aldehyde fixed material and hexazotized p-rosaniline for simultaneous coupling more than 20 different unsubstituted or substituted l-amino acid naphthylamides are split especially in the microvilli and/or stereocilia of the small intestine, kidney and epididymis from rats. Further sites of positive reactions can be revealed by l-alanyl, l-leucyl, l-lysyl, ,l-glutamyl, ,l-glutamyl, l-asparaginyl, N-benzoyl-l-arginyl, N-carbobenzoxy-l-arginyl and N-benzoyl-l-phenylalanyl 2-naphthylamide. Among the substituted and unsubstituted peptide 2-naphthylamides l-prolyl-l-arginyl 2-naphthylamide is not hydrolysed in visible amounts; l-arginyl-l-arginyl, l-alanyl-l-arginyl-l-arginyl, l-alanyl-l-leucyl-l-tyrosyl, l-histidyl-l-seryl, l-seryl-l-tyrosyl and l-glycyl-l-phenylalanyl 2-naphthylamide are metabolized in the renal and intestinal brush border; the reaction pattern obtained with N-carbobenzoxy-l-glycyl-l-glycyl-l-arginyl 2-naphthylamide differs from that of N-carbobenzoxy-l-arginyl 2-naphthylamide. In addition l-glycyl-l-prolyl, l-leucyl-l-alanyl, l-lysyl-l-alanyl and l-alanyl-l-phenylalanyl-l-prolyl 2-naphthylamide are also split in the lysosomes of many organs and the secretion granules of gland cells.

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PeptidasenI. Histochemische Untersuchungen mit 2-Naphthylamiden und Hexazonium-p-rosanilin

Zusammenfassung Mit hexazotiertem p-Rosanilin als Simultankuppler werden in frischen (mit und ohne semipermeable Membranen), gefriergetrockneten und in Schnitten von aldehyd-fixiertem Material mehr als 20 unsubstituierte oder substituierte Aminosäure-2-naphthylamide nahezu ausschließlich in den Mikrovilli und/oder Stereocilien von Dünndarm, Nebenhoden und Niere von Ratten umgesetzt. Weitere positive Reaktionsorte liefern l-Alanyl-, l-Leucyl-, l-Lysyl-, ,l-Glutamyl-, ,l-Glutamyl-, l-Asparaginyl-, N-Benzoyl-l-arginyl-, N-Carbobenzoxy-l-arginyl- und N-Benzoyl-l-phenylalanyl-2-naphthylamid. Unter den Peptid-2-naphthylamiden wird l-Prolyl-l-arginyl-2-naphthylamid nicht in sichtbarer Menge hydrolysiert; l-Alanyl-l-arginyl-l-arginyl-, l-Arginyl-l-arginyl-, l-Asparaginyl-l-arginyl-, l-Alanyl-l-leucyl-l-tyrosyl-, l-Histidyl-l-seryl-, l-Seryl-l-tyrosyl- und l-Glycyl-l-phenylalanyl-2-naphthylamid setzen der renale und intestinale Bürstensaum um. N-Carbobenzoxy-l-glycyl-l-glycyl-l-arginyl-2-naphthylamid liefert verglichen mit Benzoyl- und N-Carbobenzoxy-l-arginyl-2-naphthylamid zusätzliche Resultate. Mit l-Glycyl-l-prolyl-, l-Leucyl-l-alanyl-, l-Lysyl-l-alanyl-und l-Alanyl-l-phenylalanyl-l-prolyl-2-naphthylamid reagieren die Lysosomen zahlreicher Organe und Sekretgranula positiv.

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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 105)     

Notes on fungi from north-east India — XXII some species of hypoxylon from Assam

Summary Hypoxylon stygium (Lév)Sacc.,H. archeri Berk.,H. bovei Speg. var.microspora Miller,H. truncatum (Schw.ex Fr.)Miller,H. nummularium Bull. ex Fr. var.merillii Miller (=Hypoxylon asarcodes (Theissen)Miller?) andH. deustum (Hoff. ex Fr.)Grev. (=Ustulina zonata (Lév.)Sacc.), occurring in the tea gardens of Assam are illustrated and described. In addition, the following species ofHypoxylon hitherto reported from this country are enlisted:H. multiforme Fr. (=H. atropurpureum Fr., =H. hookeri Berk.)H. fragiforme (Pers. ex Fr.)Kickx. (=H. coccineum Bull.),H. rubiginosum Pers. ex Fr. (=H. fusco-purpureum (Schw.)Berk. et Curt., =H. nectrioides Speg., =H. perforatum Schw. ex Fr.),H. hypomiltum Mont.,H. indicum (=H. haematostroma Mont.? =H. distillatum Berk. et Fr.),H. investiens (Schw.)Curt.,H. crocopeplum Berk. et Curt. (=H. ochraceo-flavum Berk. et Cooke),H. pistillare Pers. ex Fr.,H. haematostroma Mont. (=H. vividum Berk. et Br.).H. jecorium Berk. et Rav. (=Nummularia cinnabarina P. Henn.),H. suborbiculare (=Nummularia suborbicularis (Welw. et Currey() =H. sclerophaeum Berk. et Curt? Sacc.),H. truncatum (Schw. ex Fr.)Miller (=H. annulatum (Schw.)Mont.) andH. stygium (Lév.)Sacc.     

Deuteromycetes from jabalpur-II

Summary In this paper eighteen fungi belonging to the form class Deuteromycetes are described from Jabalpur (M.P.) India. Of these one, viz.Sarcinella palawanensis (Syd.)V. P. Sahni (=Stigmella palawanensis Syd.) is described as a new combination. Among the new fungus records for India may be citedSeptoria cassiicola Kell. &Swingle onCassia fistula L.,Coniothyrium fuckelii Sacc. onAnogeissus latifolia Wall.,Phomopsis bakeri Syd. onFicus bengalensis L.,Cercospora woodfordiae Petch. onWoodfordia fruticosa (L.)Kurz.,Cercospora guanicencis Young onCaesalpinia sepiaria Roxb., andCercospora chevalieri P. Saccardo onAmorphophallus companulatus Blume.Phyllosticta buteae Syd. onButea monosperma (Lam.)Kuntze,Colletotrichum dracaenae-fragrentis (Mori)Petrak &Sydow onDracaena brachystachys Hook.,Pithomyces chartarum (Berk. &Curt.)M. B. Ellis onCassia fistula L. andCassia tora L.,Cladosporium herbarum (Pers.)Link. onButea monosperma (Lam.)Kuntze andCercospora bougainvilleae P. N. Rao onBougainvillea glabra Choisy are new state records.Discosia artocreas Tode exFr. onHolarrhena antidysentrica Wall.,Botryodiplodia theobromae Pat. onButea monosperma (Lam.)Kuntze,Colletotrichum capsici (Syd.)Butler &Bisby onButea monosperma (Lam.)Kuntze,Alternaria tenuis Nees exFr. onYucca alofolia L.,Alternaria tenuissima (Nees exFr.)Wiltshire onBougainvillea glabra Choisy, andCurvularia lunata (Wakker)Boed. onCassia tora L. are new host records.     

Production ofd-mannitol fromd-aldopentoses by the yeastRhodotorula minuta

Rhodotorula minuta produced 16% ofd-mannitol and 3% ofd-arabinitol when cultivated ond-ribose, 4% of mannitol and 11% of arabinitol when grown ond-xylose, 5% ofd-mannitol and 5% ofd-arabinitol when grown ond-arabinose, and 5% ofd-mannitol and 6% ofd-arabinitol when cultivated ond-lyxose.     

Myxomycetes from Orissa (India)-III

Summary Six myxomycetous fungi,Cribraria intricata Schrad.,Elaeomyxa miyazakiensis (Emoto)Hagelst.,Stemonitis flavogenita Jahn,Didymium vaccinum (Dur. &Mont.)Buchet,Didymium minus (Lister)Morgan andPhysarella oblonga (Berk. &Curt.)Morgan have been figured and described form Cuttack, Orissa, of whichElaeomyxa miyazakiensis (Emoto) Hagelst.,Stemonitis flavogenita Jahn,Didymium vaccinum (Dur. &Mont.)Buchet,Didymium minus (Lister)Morgan are new records for India.     

Deuteromycetes from Jabalpur-I

Summary In the present paper twelve fungi belonging to the form class Deuteromycetes are described from Jabalpur (M.P.) India. Among these the new host records areMonochaetia carissaeMunjal &Kapoor onCarissa spinarum L.,Pestalotia neglectaThuem. onAtylosia scarabaeoides,Benth.,Pestalotia japonicaSyd. onCelastrus paniculatusWilld.,Pestalotia theaeSawada var.minorStey. onButea monosperma (Lam.)Kuntze,Pestalotia versicolorSpeg. onBuchanania lanzanSpreng.,Colletotrichum dematium (Pers. exFr.)Grove onAmorphophallus sp.,Colletotrichum lindemuthianum (Sacc. &Mag.)Bri. &Cav. onCassia tora L.,Coniella diplodiella (Speg.)Petrak &Syd. on.Anogeissus latifoliaWall.,Hendersonula toruloideaNattrass onPhilodendron bipinnatifidumSchott. andMyrothecium roridumTode exFr. onCasearia tomentosaroxb. The above fungi include two species (Pestalotia neglectaThuem. andPestalotia theaeSawada var.minorStey.) which have been described for the first time from this country.Sphaeropsis tumefasciensHedges onCitrus medica L. var.acida L. is a new record from this state; andExcipularia narsapurensisSubramanian, also a new record from the state, is reported for the first time on a named host.     

Metabolism of amino acid amides in Pseudomonas putida ATCC 12633

The metabolism of the natural amino acid l-valine, the unnatural amino acids d-valine, and d-, l-phenyglycine (d-, l-PG), and the unnatural amino acid amides d-, l-phenylglycine amide (d-, l-PG-NH₂) and l-valine amide (l-Val-NH₂) was studied in Pseudomonas putida ATCC 12633. The organism possessed constitutive l-amidase activities towards l-PG-NH₂ and l-Val-NH₂, both following the same pattern of expression, suggesting the involvement of similarly regulated enzymes, or a common enzyme. Quite surprisingly, growth in mineral media with l-PG-NH₂ resulted in variable, long lag phases of growth and strongly reduced l-amidase activities. Conversion of d-PG-NH₂ into d-PG and l-PG also occurred and could be attributed to the presence of an inducible d-amidase and the racemization of the amino acid amide in combination with l-amidase activity, respectively. The further degradation of l-PG and d-PG involved constitutive l-PG aminotransferase and inducible d-PG dehydrogenase activities, respectively, both with a high degree of enantioselectivity. Amino acid racemase activity for d- and l-PG was not detected. Correspondence to: L. Dijkhuizen     

Substrate specificity of a recombinant ribose-5-phosphate isomerase from <Emphasis Type="Italic">Streptococcus pneumoniae</Emphasis> and its application in the production of <Emphasis Type="SmallCaps">l</Emphasis>-lyxose and <Emphasis Type="SmallCaps">l</Emphasis>-tagatose

A putative ribose-5-phosphate isomerase (RpiB) from Streptococcus pneumoniae was purified with a specific activity of 26.7 U mg⁻¹ by Hi-Trap Q HP anion exchange and Sephacryl S-300 HR 16/60 gel filtration chromatographies. The native enzyme existed as a 96-kDa tetramer with activity maxima at pH 7.5 and 35°C. The RpiB exhibited isomerization activity with l-lyxose, l-talose, d-gulose, d-ribose, l-mannose, d-allose, l-xylulose, l-tagatose, d-sorbose, d-ribulose, l-fructose, and d-psicose and exhibited particularly high activity with l-form monosaccharides such as l-lyxose, l-xylulose, l-talose, and l-tagatose. With l-xylulose (500 g l⁻¹) and l-talose (500 g l⁻¹) substrates, the optimum concentrations of RpiB were 300 and 600 U ml⁻¹, respectively. The enzyme converted 500 g l⁻¹ l-xylulose to 350 g l⁻¹ l-lyxose after 3 h, and yielded 450 g l⁻¹ l-tagatose from 500 g l⁻¹ l-talose after 5 h. These results suggest that RpiB from S. pneumoniae can be employed as a potential producer of l-form monosaccharides.     

Induction of aldose reductase activity inCandida guilliermondii by pentose sugars

Summary Cell extracts ofCandida guilliermondii grown ind-xylose,l-arabinose,d-galactose,d-glucose,d-mannose and glycerol as sole carbon sources possessed NADPH-dependent aldose reductase activity, but no NADH-dependent activity was detected.d-xylose andl-arabinose were the best inducers of aldose reductase activity. The highest enzyme activity ind-xylose orl-arabinose-grown cells was observed first withl-arabinose followed byd-xylose as substrates of the enzymatic reaction. However, only low activity was found ind-glucose,d-mannose andd-galactose-grown cells, indicating that these carbon sources cause catabolite repression. Enzyme activities induced ind-xylose-grown cells were twice as high as those obtained from the cells under resting conditions. Furthermore, the level of induction of aldose reductase activity depended on the initial concentration ofd-xylose. The present study shows that aldose reductase activity may be efficiently induced by pentose sugars of hemicellulosic hydrolysates and weakly by hemicellulosic hexoses.     

Some results and prospects of the utilization of beneficial entomophagous insects in the control of insect pests in Georgian SSR (USSR)

Résumé Nous avons brièvement indiqué dans cette note les travaux de lutte biologique entrepris en Géorgie par introduction d'entomophages parasites et prédateurs contre des Insectes nuisibles également introduits auparavant. Nous avons particulièrement souligné l'importance deAphelinus mali Muls. contreEriosoma lanigerum Hausm.,Rodalia cardinalis Muls. contreIcerya purchasi Mask.,Cryptolaemus montrouzieri Muls. contrePseudococcus gahani Green,Lindorus lophanthae Blaisdell contre une série de Diaspidides (Aspidiotus hederae Vall.,A. destructor Sign.,Chrysomphalus dictyospermi (Morg.),Aonidiella citrina Coq.),Prospaltella berlesi How. contrePseudaulacaspis pentagona Targ.,Pseudaphycus malinus contrePseudococcus comstocki (Kuw.),Leptomastix dactylopii How. et Leptomastidea abnormis (Gyr.) contrePseudococcus vitis Niet.,Rhizophagus grandis Gyll. contreDendroctonus micans Kugel.      

Regulation of reduced nitrogen assimilation in Rhodobacter capsulatus E1F1

The phototrophic bacterium Rhodobacter capsulatus E1F1 assimilates ammonia and other forms of reduced nitrogen either through the GS/GOGAT pathway or by the concerted action of l-alanine dehydrogenase and aminotransferases. These routes are light-independent and very responsive to the carbon and nitrogen sources used for cell growth. GS was most active in cells grown on nitrate or l-glutamate as nitrogen sources, whereas it was heavily adenylylated and siginificantly repressed by ammonium, glycine, l-alanine, l-aspartate, l-asparagine and l-glutamine, under which conditions specific aminotransferases were induced. GOGAT activity was kept at constitutive levels in cells grown on l-amino acids as nitrogen sources except on l-glutamine where it was significantly induced during the early phase of growth. In vitro, GOGAT activity was strongly inhibited by l-tyrosine and NADPH. In cells using l-asparagine or l-aspartate as nitrogen source, a concerted induction of l-aspartate aminotransferase and l-asparaginase was observed. Enzyme level enhancements in response to nitrogen source variation involved de novo protein synthesis and strongly correlated with the cell growth phase.Abbreviations ADH l-alanine dehydrogenase - AOAT l-alanine:2-oxoglutarate aminotransferase - Asnase l-asparaginase - GOAT Glycine: oxaloacetate aminotransferase - GOGAT Glutamate synthase - GOT l-aspartate: 2-oxoglutarate aminotransferase - GS Glutamine synthetase - HPLC High-Pressure Liquid Chromatography - MOPS 2-(N-morpholino)propanesulfonic acid - MSX l-methionine-d,l-sulfoximine     

Racemic Resolution of some <Emphasis Type="SmallCaps">dl</Emphasis>-Amino Acids using <Emphasis Type="Italic">Aspergillus fumigatus</Emphasis><Emphasis Type="SmallCaps">l</Emphasis>-Amino Acid Oxidase

The ability of Aspergillus fumigatus l-amino acid oxidase (l-aao) to cause the resolution of racemic mixtures of dl-amino acids was investigated with dl-alanine, dl-phenylalanine, dl-tyrosine, and dl-aspartic acid. A chiral column, Crownpak CR+ was used for the analysis of the amino acids. The enzyme was able to cause the resolution of the three dl-amino acids resulting in the production of optically pure d-alanine (100% resolution), d-phenylalanine (80.2%), and d-tyrosine (84.1%), respectively. The optically pure d-amino acids have many uses and thus can be exploited industrially. This is the first report of the use of A. fumigatus l-amino acid oxidase for racemic resolution of dl-amino acids.     

A phylogenetic analysis of the lichen familySphaerophoraceae (Caliciales); a new generic classification and notes on character evolution

A phylogenetic analysis of the familySphaerophoraceae (Caliciales, lichenized ascomycetes) has resulted in a new generic classification. Notes on character evolution are given. The generaSphaerophorus s. str.,Bunodophoron andLeifidium, gen. nov., are accepted.Pleurocybe andPseudosphaerophorus are considered synonyms ofBunodophoron andThysanophoron is considered synonym toSphaerophorus. The following new combinations are proposed:Bunodophoron coomerense (Ohlsson)Wedin,B. diplotypum (Vain.)Wedin,B. dodgei (Ohlsson)Wedin,B. flaccidum (Kantvilas & Wedin)Wedin,B. formosanum (Zahlbr.)Wedin,B. imshaugii (Ohlsson)Wedin,B. insigne (Laurer)Wedin,B. kinabaluense (M. Satô)Wedin,B. macrocarpum (Ohlsson)Wedin,B. madagascareum (Nyl.)Wedin,B. microsporum (Ohlsson)Wedin,B. murrayi (Ohlsson)Wedin,B. notatum (Tibell)Wedin,B. ohlssonii (Wedin)Wedin,B. patagonicum (C. W. Dodge)Wedin,B. ramuliferum (I. M. Lamb)Wedin,B. scrobiculatum (C. Bab.)Wedin,B. tibellii (Wedin)Wedin,B. whakapapaense (Wedin)Wedin, andLeifidium tenerum (Laurer)Wedin.     

Additions to the fungi of Jabalpur (madhya pradesh) V

Summary The present paper includes ten parasitic fungi occurring at Jabalpur. These include three new species viz.Hendersonia syzygii Hasija onSyzygium cumini, Phoma ixorae Hasija onIxora sp. andPhyllosticta agarwalii Hasija onDalbergia paniculata. Colletotrichum truncatum (Schw.)Andrus &Moore onAlysicarpus bupleurifolius andPiricularia zingiberi onHedychium sp. are new fungus records for the country.Grewia hirsuta forPhyllosticta sedgwickii DaCosta &Mundkur,Lagerstroemia speciosa forPestalotiopsis versicolor (Speg.)Stey.,Zingiber officinale forCurvularia lunata (Wakker)Boedijn,Musa paradisiaca forDeightoniella torulosa (Syd.)Ellis andFlacourtia ramontchi forAlternaria tenuis Nees exPers. are new hosts record from Jabalpur, India.     

Miscellanea hepaticologica 101–110

Summary 101.Blepharidophyllum clandestinum (Mont.)Lacoutore 1910 instead of (Mont.)Grolle 1965. 102.Clasmatocolea rotata (H. f & T. 1844) c. n.=C. alpina (Rodway 1916)Grolle 1966, syn. nov. 103.Isotachis armata (Nees 1844)Gottsche 1864=Lophocolea laxissima Herz. 1932, syn. nov. 104.Leptodon smithii (Hedw.)Web. &Mohr 1803=Jungermannia nervosa Spreng. 1827, syn. nov. 105.Leptoscyphus abditus (Sull.)Dugas 1928 instead of (Sull.)Grolle 1962. 106.L. amphibolius (Nees 1833)Grolle 1962=Chiloscyphus dorsilobus Nees 1845, syn. nov. 107.L. cuneifolius (Hook.)Mitt. new to Venezuela. 108.L. horizontalis (Hook.)Kühnemann 1937 instead of (Hook.)Grolle1962. 109.L. porphyrius (Nees 1845) c. n.=Jungermannia paupercula Tayl. 1846, syn. nov.;=J. liebmaniana L. & G. 1847, syn. nov.=L. liebmanianus (L. & G.)Mitt. 110.Lethocolea amplexifolia (Hampe exLehm.) C. n.=Plagiochila amplexifolia Hampe Ex Lehm. 1838. Notes on marsupia and gemmae ofLethocolea and their taxonomic value.     

Alterations in <Emphasis Type="SmallCaps">D</Emphasis>-amino acid levels in the brains of mice and rats after the administration of <Emphasis Type="SmallCaps">D</Emphasis>-amino acids

Summary. To mutant ddY/DAO⁻ mice lacking D-amino-acid oxidase activity and normal ddY/DAO⁺ mice, five D-amino acids (D-Asp, D-Ser, D-Ala, D-Leu and D-Pro) were orally administered for two weeks, and the D-amino acid levels were examined in seven brain regions. The levels of D-Asp markedly increased in the pituitary and pineal glands in both strains. In the ddY/DAO⁺ mice, the levels of the other D-amino acids did not significantly change in most of the brain regions. While in the ddY/DAO⁻ mice the levels of D-Ser significantly increased in most of the brain regions except for the cerebrum and hippocampus. The levels of D-Ala and D-Leu increased in all regions but the levels of D-Pro did not significantly change. The same five D-amino acids were intravenously injected into Wistar rats and the D-amino acid levels in their brains were examined for 60 min after the administration. The levels of D-Asp markedly increased in the pineal gland 3 min after the administration, while the levels of D-Ser, D-Ala, and D-Pro increased both in the pineal and pituitary glands, the levels of D-Leu increased in all brain regions. These results are useful for the elucidation of the origins and regulation of D-amino acids in the mammalian body.     

Growth inhibition by L-phenylalanine in Agmenellum quadruplicatum

Summary L-Phenylalanine is a potent inhibitor of growth in a marine species of blue-green bacteria, Agmenellum quadruplicatum. The growth inhibition is reversed by many amino acids when added to the culture medium simultaneously with L-phenylalanine. The most effective L-phenylalanine antagonists are L-tyrosine, L-alanine, L-leucine, L-methionine, L-tryptophan, and L-isoleucine. However, L-tyrosine is the only effective L-phenylalanine antagonist when growth is inhibited by L-phenylalanine for two or more hours prior to addition of an equimolar concentration of the compound tested as an antagonist. Various explanations that could account for inhibition of growth by L-phenylalanine are discussed. Inhibition of growth by L-phenylalanine is not a feature peculiar to the general physiology of blue-green bacteria. For example, the growth of Anacystis nidulans, a fresh water species, was not inhibited by L-phenylalanine, although a different pattern of metabolite sensitivity was found.     

Characterization of prolidase I and II purified from normal human erythrocytes: comparison with prolidase in erythrocytes from a patient with prolidase deficiency

The effect of various sulfur-containing amino acids on the activities of prolidase isoenzymes I and II isolated from erythrocytes of healthy individuals, and erythrocyte lysates from a patient with prolidase deficiency was investigated. The activity of prolidase I against glycylproline was strongly enhanced by d-methionine. l-Methionine and d,l-methionine slightly enhanced the activity at low concentration, but N-acetyl-l-methionine had no effect. d-Ethionine, l-ethionine, and d,l-ethionine also enhanced the activity of prolidase I. d,l-Homocysteine enhanced the activity at low concentration, but inhibited the activity at 50 mM. The activity of prolidase II against methionylproline was enhanced by d-methionine, d,l-methionine, and l-methionine, but N-acetyl-l-methionine had no effect. d-Ethionine and d,l-ethionine strongly enhanced the activity of prolidase II compared with l-ethionine; d,l-homocysteine weakly enhanced the activity. d,l-Homocysteine-thiolactone inhibited the activities of prolidase I and II in a concentration-dependent manner. The effect of various sulfur-containing amino acids on prolidase activity against methionylproline in erythrocyte lysates from a patient with prolidase deficiency was almost the same as that on prolidase II. The kinetics of the activities of prolidase I, II, and patient prolidase were also studied. Their K _m values were changed by adding sulfur-containing amino acids, but V _max values were unchanged.     

Novos Deuteromycetes da micogeografia intercontinental

Summary This paper deals with some interesting new fungi which are described as a contribution for the development of the Mycogeography.ArbusculaBatista &Peres n. gen. is studied as a member of the family Stilbaceae havingA. eugeniaeBatista &Peres n. sp. on leaves ofEugenia jambolanaLam. from Lucknow, India, as the type species. Another new genus isEllisiaBatista &Peres, typified byE. ingaeBatista &Peres n. sp. on leaves ofInga fagifolia (L.)Willd. from Manaus. Amazonas, Brazil. Two new binomia are:Eriomycopsis paraensisBatista &Peres n. sp. on leaves ofBertholletia excelsa H.B.K. from Belem, State of Pará, Brazil,Sporidesmium americanenseBatista &Peres n. sp. on leaves ofQuercus wislizenii A.D.C., from U.S.A. andSporidesmium cookei (Hughes)W. B. Ellis, on leaves of Lauraceae, from Manaus.

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Zusammenfassung Diese Arbeit bringt einige interessante neue Pilze, welche als Beitrag zur Entwicklung der Mycogeographie beschrieben werden.ArbusculaBatista &Peres n. gen. wurde studiert als ein Mitglied der Familie Stilbaceae, dessen TypusA. eugeniaeBatista &Peres n. sp. sich auf Blättern vonEugenia jambolanaLam. aus Lucknow, Indien, befand. Eine andere neue Gattung istEllisiaBatista &Peres, typiziert durchE. ingaeBatista &Peres n. sp. auf Blättern vonInga fagifolia (L.)Willd. aus Manaus, Amazonas, Brasilien. Zwei neue Binome sind:Eriomycopsis paraensisBatista &Peres n. sp. auf Blättern vonBertholletia excelsa H.B.K. aus Belém, Staat Pará, Brasilien,Sporidesmium americanenseBatista &Peres n. sp. auf Blättern vonQuercus wislizenii A.D.C. aus U.S.A. undSporidesmium cookei (Hughes)W. B. Ellis, auf Blättern von Lauraceae, aus Manaus, Amazonas.

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Dedicado à Mrmória do Prof.Raffaele Ciferri     

A Metal Ion as a Cofactor Attenuates Substrate Inhibition in the Enzymatic Production of a High Concentration of <Emphasis Type="SmallCaps">D</Emphasis>-glutamate Using <Emphasis Type="Italic">N</Emphasis>-acyl-<Emphasis Type="SmallCaps">D</Emphasis>-glutamate Amidohydrolase

N-Acyl-D-glutamate amidohydrolase (D-AGase) was inhibited by 94 % when 1 mol/l N-acetyl-DL- glutamate was used as a substrate. The addition of 1 mM Co²⁺ stabilized D-AGase. Moreover, the substrate inhibition was weakened to 88% with the addition of 0.4 mM Co²⁺ to the reaction mixture. Although D-AGase is a zinc-metalloenzyme, the addition of Zn²⁺ from 0.01 to 10 mM did not increase the D-glutamic acid production in the saturated substrate. Under optimal conditions, 0.38 M D-glutamic acid was obtained from N-acyl-DL-glutamate with 100% of the theoretical yield after 48 h.