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Inhibition of the RelA(p65) NF-kappaB subunit by Egr-1 总被引:9,自引:0,他引:9
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The Lrp family of transcriptional regulators 总被引:1,自引:0,他引:1
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Mutational analysis of the DNA-binding domain of the CYS3 regulatory protein of Neurospora crassa. 总被引:3,自引:2,他引:1 下载免费PDF全文
cys-3, the major sulfur regulatory gene of Neurospora crassa, activates the expression of a set of unlinked structural genes which encode sulfur catabolic-related enzymes during conditions of sulfur limitation. The cys-3 gene encodes a regulatory protein of 236 amino acid residues with a leucine zipper and an upstream basic region (the b-zip region) which together may constitute a DNA-binding domain. The b-zip region was expressed in Escherichia coli to examine its DNA-binding activity. The b-zip domain protein binds to the promoter region of the cys-3 gene itself and of cys-14, the sulfate permease II structural gene. A series of CYS3 mutant proteins obtained by site-directed mutagenesis were expressed and tested for function, dimer formation, and DNA-binding activity. The results demonstrate that the b-zip region of cys-3 is critical for both its function in vivo and specific DNA-binding in vitro. 相似文献
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