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1.
Bruno P. Berto Hermes Ribeiro Luz Walter Flausino Ildemar Ferreira Carlos W. G. Lopes 《Systematic parasitology》2009,74(1):75-80
In the current study, two new coccidian species (Protozoa: Apicomplexa: Eimeriidae) obtained from short-crested flycatcher
Myiarchus ferox (Gmelin) are reported from Brazil. Isospora feroxis n. sp. has o?cysts which are spheroidal to sub-spheroidal, 18.7 × 18.0 μm, with a smooth and bi-layered wall, c. 1.2 μm. The micropyle and o?cyst residuum are absent, but two polar granules are present. Its sporocysts are broadly ellipsoidal
and 11.7 × 8.5 μm. Stieda and substieda bodies are present. A sporocyst residuum is present and the sporozoites have a refractile
body and nucleus. O?cysts of Eimeria sicki n. sp. are spheroidal to sub-spheroidal, 30.3 × 28.5 μm, with a smooth and bi-layered wall, c. 1.3 μm. The micropyle, o?cyst residuum and polar granule are absent. Its sporocysts are ellipsoidal, 18.4 × 10.0 μm. Stieda
and substieda bodies are present. A sporocyst residuum is present and sporozoites have a refractile body and nucleus. 相似文献
2.
Two new nematode species, Ascarophis longiovata n. sp. and Neoascarophis longispicula n. sp. (Cystidicolidae), are described from the digestive tract of the marine deep-water fish, the Mediterranean grenadier
Coryphaenoides mediterraneus (Giglioli), from the Mid-Atlantic Ridge. The former species is characterised mainly by the structure of the mouth (large
pseudolabia, each with well-developed dorsal and ventral extension and small apical protrusion; submedian labia almost absent),
the large, elongate-oval, non-filamented eggs (60–66 × 18–27 μm), a cervical inflation of the cuticle, bifurcate deirids,
and the length of the spicules (315–360 and 120–147 μm), whereas the latter (only males available) can be distinguished by
the length of the spicules (960–1,149 and 258–351 μm) and their length ratio (1:1.91–2.71), the shape of the deirids (bifurcate,
with long, narrow posterior arms), and the location of the excretory pore and deirids well posterior to the level of the nerve-ring. 相似文献
3.
Hiroto Suhara Nitaro Maekawa Shuji Ushijima Kazuhiko Kinjo Yoshikazu Hoshi 《Mycoscience》2010,51(1):75-80
A new homobasidiomycete, Asterostroma macrosporum, was found in mangrove forests of Iriomote Island, Japan. This species is morphologically characterized by having resupinate
basidiomata, a monomitic (asterodimitic) hyphal system, simple septate generative hyphae, dextrinoid asterosetae, four sterigmate
basidia and globose, tuberculate and amyloid basidiospores measuring 8.5–11 × 7.5–9 μm. It is similar to A. muscicola, but basidiospores in the latter are smaller (7–8 × 5.5–7 μm). Furthermore, phylogenetic analysis using internal transcribed
spacer (ITS) region revealed that A. macrosporum is distinctly separated from A. muscicola. In Japan, A. muscicola is widely distributed in warm-temperate to subtropical regions, growing on a variety of broadleaved trees including mangroves,
while A. macrosporum has been found only on mangroves. 相似文献
4.
A new species, Overstreetia olsoni, is described from Atherinomorus capricornensis off Heron Island on the Great Barrier Reef off Queensland, Australia. It differs from its only known congener in that the enlarged spines of the circum-oral rows are smaller ( ≤ 4.5 × 3.5 vs ≤ 21.5 × 10.5μm), the sucker-ratio is smaller (1: 0.97–1.01 vs 1:1.8) and the eggs are larger (40–45 × 22–28 vs 34–37 × 17–22μm). 相似文献
5.
Plant regeneration through somatic embryogenesis was achieved using immature zygotic embryos (ZE) of Sorbus pohuashanensis as explants. Over 50% of immature ZEs from immature seed collected at 30 days after pollination produced direct somatic embryos
(SEs) on Murashige and Skoog (MS) medium supplemented with 0–0.44 μM 6-benzyladenine (BA) in combination with 5.73 μM naphthaleneacetic
acid (NAA) or with 0.91–2.26 μM 2,4-dichlorophenoxyacetic acid (2,4-D) alone. Fourteen to 23 SEs per explant were regenerated
on MS medium supplemented with BA 0.44 μM in combination with NAA 5.73 μM. SE formation decreased when sucrose concentrations
were higher than 40 g L−1. Repetitive embryogenesis occurred following culture on solid MS medium containing 12 μM abscisic acid, 75 g L−1 polyethylene glycol, and 20 g L−1 sucrose at 25 ± 1°C under a 16-h photoperiod with a light intensity of 40 μmol m−2 s−1. Over 40% of the mature SEs germinated on solid MS medium under light condition described previously. Up to 40% of the regenerated
plantlets were successfully acclimatized under greenhouse conditions. Plantlets derived from SEs grew vigorously with similar
morphology as those germinated from ZEs. Histological studies of explants at various developmental stages of somatic embryogenesis
revealed that SEs passed through globular, heart, torpedo, and mature stages. Similar to ZE suspensors, similar structures
of SE degenerated in later stages of embryo development. ZE and SE are a effective means of regenerating tissue culture plantlets
for S. pohuashanesis. 相似文献
6.
Total synaptonemal complex (SC) lengths were estimated from Oreochromis aureus Steindachner (which has a WZ/ZZ sex determination system), O. mossambicus Peters and O. niloticus L. (both of which have XX/XY sex determination systems). The total SC length in oocytes was greater than that in spermatocytes
in all three species (194 ± 30 μm and 134 ± 13 μm, 187 ± 22 μm and 127 ± 17 μm, 193 ± 37 μm and 144 ± 19 μm, respectively).
These sex-specific differences did not appear to be influenced by the type of sex determination system (the female/male total
SC length ratio was 1.45 in O. aureus, 1.47 in O. mossambicus and 1.34 in O. niloticus) and do not correlate with the lack of any overall sex-specific length differences in the current Oreochromis linkage map. Although based on data from relatively few species, there appears to be no consistent relationship between sex-specific
SC lengths and linkage map lengths in fish. Neomale (hormonally masculinized genetic female) O. aureus and O. mossambicus had total SC lengths of 138 ± 13 μm and 146 ± 13 μm respectively, more similar to normal males than to normal females. These
findings agree with data from other vertebrate species that suggest that phenotypic sex, rather than genotype, determines
traits such as total SC length, chiasmata position and recombination pattern, at least for the autosomes. 相似文献
7.
The reproductive biology of the Tristan klipfish, Bovichtus diacanthus, was investigated by macroscopic and histological analyses of the gonads. Fish samples were collected in tide pools at Tristan
da Cunha in July 2004. Most specimens of both sexes were developing, or sexually mature, with a gonadosomatic index (GSI)
of 7.0–9.2% in females and 0.2–0.6% in males. Histologically, testes showed a random distribution of spermatogonia along the
lobules, a condition defined as the unrestricted spermatogonial type. Ripe males exhibited lobules with all spermatogenic
stages of development from spermatogonia to spermatozoa. In mature females, the ovarian follicles consisted of three main
cohorts of oocytes of different sizes; the smaller one represented by previtellogenic oocytes of 15–150 μm and the other two
by yolked oocytes measuring, respectively, 300–1000 and 800–1500 μm. The overlap between the stock of advanced yolked oocytes
and the early yolked oocytes was low, decreasing progressively with final maturation. As a result, B. diacanthus was considered a batch spawner, with a spawning season extending from July to August onward. Batch fecundity, based on the
most advanced yolked oocytes, was 2,047–8,317 mature oocytes/female, whereas the relative fecundity was 77–141 mature oocytes/g.
In the light of the phyletically basal position of bovichtids in the suborder, the reproductive traits of B. diacanthus were compared with those previously described in other Antarctic and non-Antarctic notothenioids. 相似文献
8.
Oviposition by western jewel butterflies (Hypochrysops halyaetus Hewitson) was studied in the urban Koondoola regional bushland reserve, Western Australia in 1999. Observations were made
in a small (70 × 120 m) localised breeding area the ‘microdistribution study area’, and in 14 paired 20 × 20 m quadrats randomly
placed along established tracks in the interior of the northern half of the reserve. The principal host plant was Jacksonia
sternbergiana with the secondary host being Daviesia
divaricata. More Jacksonia were used for oviposition and attended by ants (40 and 71%, respectively) in the microdistribution study than plants in the
‘quadrat’ study (8 and 25%, respectively). Jacksonia stems with basal diameters below 5 mm were less likely to have eggs. PCA showed Jacksonia to be associated with disturbed conditions with a high proportion of bare ground whereas Daviesia was associated with more mature vegetation. Jacksonia density was principally correlated with the proportion of bare ground and time since the last fire. Host ant (Crematogaster
perthensis) presence and proportion of bare ground were the most important factors influencing oviposition. The presence of ants was
closely associated with bare ground conditions and the presence of coccids. Conservation management for floristic diversity
and maturity of vegetation in reserves is likely to be a widespread phenomenon, and may negatively affect the persistence
of species requiring ephemeral patches of early successional vegetation. For H. halyaetus, a species dependent on disturbed/ephemeral habitat conditions, this conflict may threaten its survival, especially in small
reserves, and as a result its conservation status may be underestimated. Management options are discussed. 相似文献
9.
Codium fragile (Suringar) Hariot is an edible green alga farmed in Korea using seed stock produced from regeneration of isolated utricles
and medullary filaments. Experiments were conducted to reveal the optimal conditions for nursery culture and out-growing of
C. fragile. Sampling and measurement of underwater irradiance were carried out at farms cultivating C. fragile at Wando, on the southwestern coast of Korea, from October 2004 to August 2005. Growth of erect thalli and underwater irradiance
were measured over a range of depths for three culture stages. During the nursery cultivation stage (Stage I), growth rate
was greatest at 0.5 m depth (0.055 ± 0.032 mm day−1), where the average midday irradiance over 60 days was 924 ± 32 μmol photons m−2 s−1. During the pre-main cultivation stage (Stage II), the greatest growth rate occurred at a depth of 2 m (0.113 ± 0.003 mm day−1) with an average irradiance of 248 ± 116 μmol photons m−2 s−1. For the main cultivation stage (Stage III) of the alga, thalli achieved the greatest increase in biomass at 1 m depth (7.2 ± 1.0 kg
fresh wt m−1). These results suggest that optimal growth at each cultivation stages of C. fragile could be controlled by depth of cultivation rope. 相似文献
10.
The regeneration of meristematic tissues from sporophytes of Laminaria digitata was studied by protoplast and tissue culture. Sequential treatment of explants in sterile seawater with 1% Betadine for 5 min,
1% commercial bleach for 1–2 min and 2% antibiotic treatment supplemented with 1 μM GeO2 overnight enabled viable explants as high as 55%. Different morphogenetic responses were observed from tissue culture on
media supplemented with plant growth regulators alone or in combination, mainly filamentous calluses up to 50% according to
the media. Dark green compact calluses were observed on two combinations: 4 μM Pi + 2 μM N-(2-chloro-4-pyridyl)-N’-phenylurea
(CPPU) and 0.04 μM Pi + 0.44 μM 6-benzylaminopurine. Thalloid-like structures comparable to adventitious buds were regenerated
on medium supplemented with 4 μM Pi + 0.45 μM zeatin but at low frequency suggesting a strong genotypic effect. Friable calluses
were developed from protoplasts in enriched medium with polyamines and containing 0.40 μM CPPU + 0.45 μM 2,4-dichlorophenoxyacetic
acid. In order to produce protoplasts, a one-step enzymatic protocol was developed and yields reached 22 × 106 protoplasts per gram of fresh weight. 相似文献
11.
The distribution of summer phytoplankton across the Straits of Magellan (SOM) was studied with the aims of tracing differences
among the distinct subregions of the area and contributing to the knowledge of its biodiversity. Samples collected at 25 stations
were observed and counted in light microscopy. Selected samples were observed with transmission electron microscopy. The main
unifying feature of the phytoplankton in the SOM was the high abundance and numerical dominance of small-sized (<10 μm) eukaryotic
species, among which coccoid cells of <3 μm size were predominant (56.2 ± 30.6 of the total phytoplankton abundance). They
mostly belonged to the prasinophyte Pycnococcus provasolii, which was abundant (0.8–6,834 cells × 103 ml−1) at all stations with the exception of those in proximity to the Atlantic entrances, where it was not recorded. Small-sized
(<3 and 3–5 μm) diatoms (Minidiscus trioculatus, Lennoxia faveolata and other undetermined centric species) attained high densities (<3,757 cells 103 ml−1) especially at stations of the Patagonian sectors, whereas microplanktonic diatoms were only found at the two entrances of
the Straits. Dinoflagellates were constituted mainly by >10 μm forms in the Andean subregion and <10 μm naked species in the
Patagonian subregion, contributing up to 75.9 and 41.8% of the total carbon in these two areas, respectively. In the Patagonian
subregion, flagellates mainly constituted by <5 μm forms and by cryptomonads <10 μm comprised up to 53.9% of the total biomass.
Several species identified in this study have never been reported in other investigations in the SOM, while others, including
Pycnococcus provasolii and Lennoxia faveolata, have rarely been recorded elsewhere. Overall, the summer phytoplankton of the Straits does not resemble that of any other
region of the world’s seas. Although some of the predominant species might have been overlooked elsewhere, their abundance
and relative importance apparently constitute a distinctive feature of the SOM. 相似文献
12.
Vishal Gupta Manoj Kumar Puja Kumari C. R. K. Reddy Bhavanath Jha 《Journal of applied phycology》2011,23(2):209-218
This study reports on the optimization of protoplast yield from two important tropical agarophytes Gracilaria dura and Gracilaria verrucosa using different cell-wall-degrading enzymes obtained from commercial sources. The conditions for achieving the highest protoplast
yield was investigated by optimizing key parameters such as enzyme combinations and their concentrations, duration of enzyme
treatment, enzyme pH, mannitol concentration, and temperature. The significance of each key parameter was also further validated
using the statistical central composite design. The enzyme composition with 4% cellulase Onozuka R-10, 2% macerozyme R-10,
0.5% pectolyase, and 100 U agarase, 0.4 M mannitol in seawater (30‰) adjusted to pH 7.5 produced the highest protoplast yields
of 3.7 ± 0.7 × 106 cells g−1 fresh wt for G. dura and 1.2 ± 0.78 × 106 cells g−1 fresh wt for G. verrucosa when incubated at 25°C for 4–6 h duration. The young growing tips maximally released the protoplasts having a size of 7–15 μm
in G. dura and 15–25 μm in G. verrucosa, mostly from epidermal and upper cortical regions. A few large-size protoplasts of 25–35 μm, presumably from cortical region,
were also observed in G. verrucosa. 相似文献
13.
Proacrosomal vesicles form during the pachytene stage, being synthetized by the Golgi complex in Glycymeris sp., and by both the Golgi and the rough endoplasmic reticulum in Eurhomalea rufa. During early spermiogenesis, a single acrosomal vesicle forms and its apex becomes linked to the plasma membrane while it
migrates. In Glycymeris sp., the acrosomal vesicle then turns cap-shaped (1.8 μm) and acquires a complex substructure. In E. rufa, proacrosomal vesicles differentiate their contents while still at the premeiotic stage; as the acrosomal vesicle matures
and its contents further differentiate, it elongates and becomes longer than the nucleus (3.2 μm), while the subacrosomal
space develops a perforatorium. Before condensation, chromatin turns fibrillar in Glycymeris sp., whereas it acquires a cordonal pattern in E. rufa. Accordingly, the sperm nucleus of Glycymeris sp. is conical and elongated (8.3 μm), and that of E. rufa is short and ovoid (1.1 μm). In the midpiece (Glycymeris sp.: 1.1 μm; E. rufa: 0.8 μm), both species have four mitochondria encircling two linked orthogonal (Glycymeris sp.) or orthogonal and tilted (30–40°; E. rufa) centrioles. In comparison with other Arcoida species, sperm of Glycymeris sp. appear distinct due to the presence of an elongated nucleus, a highly differentiated acrosome, and four instead of five
mitochondria. The same occurs with E. rufa regarding other Veneracea species, with the acrosome of the mature sperm strongly resembling that of the recent Mytilinae.
Electronic Publication 相似文献
14.
Neslihan Balkis 《Aquatic Ecology》2009,43(2):249-270
Seasonal changes in the microphytoplankton assemblages were examined in the coastal zone of Bozcaada Island with regard to
some major physical and chemical variables. Samples were collected from May 2000 to December 2001 at four stations. A total
of 108 dinoflagellates, 102 diatoms, 1 chrysophycean, 3 dictyochophycean, and 1 prasinophycean species were identified and
quantified during the study period. Diatoms and dinoflagellates were the most important in terms of species number and abundance.
The maximum values of total microphytoplankton were observed at 0.5 m depth (46.2 × 103 cells l−1 at st. 3) in May as this was the month when the diatom Pseudo-nitzschia pungens bloomed. Chlorophyll (chl) a concentration ranged between 0.08 (August) and 0.78 μg l−1 (February). May was another important month in which chlorophyll a increased (0.41–0.47 μg l−1). Species diversity values (H′log2) ranged from 1.66 bits (June, 20 m) to 4.11 bits (November, 0.5 m). The increase was attributed to a more balanced distribution
of abundance among species. The amounts of nitrate + nitrite (0.6−3.7 μg-at N l−1), phosphate (0.2−0.6 μg-at P l−1) and silicate (0.7−2.5 μg-at Si l−1) were recorded on each sampling occasion. Nutrient concentrations and chl a values of the research area were found to be poorer than those of the many other coastal areas in the northeastern Mediterranean.
The mean atomic ratio of nitrogen to phosphorus varied from 1.3 (June) to 12.9 (February). This ratio was lower than the Redfield
ratio of 16 for ocean phytoplankton, and phytoplankton was potentially limited by nitrogen for most of the months. The result
of this study confirms and emphasizes the oligotrophic nature of the eastern Mediterranean. 相似文献
15.
Protoplasts were isolated enzymatically from the carrageenophyte red alga Grateloupia turuturu (Halymeniales, Rhodophyta) that occurs along the coast of the French Channel in Normandy. Effects of the main factors on
the protoplast yield were identified to improve the isolation protocol. The optimal enzyme composition for cell wall digestion
and protoplast viability consisted of 2% cellulase Onozuka R-10, 0.5% macerozyme R-10, 2% crude extract from viscera of Haliotis tuberculata, 0.8 M mannitol, 20 mM sodium citrate, 0.3% bovine serum albumin at 25°C, and 4-h incubation period. The protoplasts were
approximately 5–15 μm in diameter, liberated mainly from the surface cell layers. Maximum yield was 1.5 × 107 protoplasts g-1 fresh tissue. The protoplasts underwent initial division after 14 days with a high density level of 1 × 106 cells mL-1 in culture medium and developed into microthalli of a line of two to six cells. 相似文献
16.
17.
Batai (Falcataria moluccana) is a valuable tree species for forest plantations in Malaysia and Indonesia. Since 1993, a gall rust disease has caused
severe damage to all growth stages, from seedlings in the nursery to mature trees in the field. To identify the fungus causing
gall rust disease on F. moluccana in Malaysia and Indonesia, study of the mode of infection and changes in the anatomy of infected cells were carried out in
the anatomy laboratory. The disease in Malaysia and Indonesia is caused by Uromycladium tepperianum. The fungus produces three longitudinally ridged teliospores on each head, with spores measuring 13–20 μm wide and 17–28 μm
long. The fungus is microcyclic, completing its entire life cycle on F. moluccana. This study confirmed that the teliospores themselves cannot infect the host. Under favorable conditions, about 10 h after
inoculation, teliospores germinate to produce basidiospores that form penetration pegs about 6 h later, and it is this peg
which penetrates the host cells directly through the epidermis. Pycnia, recognized as small brown pustules, break through
the epidermis about 7 days after inoculation. 相似文献
18.
Production rates, abundance, chlorophyll a (Chl a) concentrations and pigment composition were measured for three size classes (<2 μm, 2–11 μm and >11 μm) of phytoplankton
from May to December 2000 in deep, mesotrophic, alpine lake Mondsee in Austria. The study focuses on differences among phytoplankton
size fractions characterised by their surface area to volume ratio ([mm2 l−1: mm3l−1]), pigment distribution patterns and photosynthetic rates. Particular attention was paid to autotrophic picophytoplankton
(APP, fraction <2 μm) since this size fraction differed significantly from the two larger size fractions. Among the three
fractions, APP showed the highest surface area to volume ratios and a high persistence in the pattern of lipophilic pigments
between temporarily and spatially successive samples (about 80% similarity of pigment composition between samples over seasons
and depths). The epilimnetic abundance of APP varied seasonally with an annual maximum of 180 × 103 cells ml−1 in June (at 4–9 m). The minimum (October at 12 m) was more than an order of magnitude lower (4.9 × 103 ml−1). APP peaked during autumn and contributed between 24% and 42% to the total area-integrated Chl a (10–23 mg m−2) and between 16% and 58% to total area-integrated production (5–64 mg m−2 h−1) throughout seasons. 相似文献
19.
Kadie B. Heinle Douglas L. Larson Andrew M. Lockwood Edward A. Baker Kim T. Scribner 《Zeitschrift fur angewandte Ichthyologie》2020,36(2):151-158
Previous molecular diet analysis identified lake sturgeon (Acipenser fulvescens, Rafinesque, 1817) DNA in the gastrointestinal tracts of stream-resident rainbow darters (Etheostoma caeruleum, Storer, 1845) during the egg incubation, free embryo, and larval drift stages. The objectives of this experimental study were to: (a) quantify levels of predation by rainbow darters on lake sturgeon at the egg and free-embryo stages; and (b) evaluate whether predation varied as a function of substrate size and rainbow darter body size. We conducted experimental trials in 23-L polycarbonate tanks 0.41 m (L) × 0.33 m (W) × 0.30 m (D) with a standardized benthic area of 0.14 m2. The tanks were randomly assigned one of two different substrate size classes: large rock (51.35 mm ± 0.91 mm) or small rock (27.68 mm ± 0.57 mm). We stocked individual rainbow darter, which were deprived of feed for 48 hr, with lake sturgeon (133 individuals/m2) in each of 12 replicates per ontogenetic stage and substrate type. The number of surviving lake sturgeon was quantified following a 24-hr predation exposure period. We used a generalized linear model with a binomial distribution to assess the influence of ontogenetic stage, substrate size, and rainbow darter body size on proportional lake sturgeon survival. Predation on lake sturgeon occurred at both egg (6.25 ± 1.16 individuals, mean ± 2SE) and free embryo (3.08 ± 1.08 individuals, mean ± 2SE) stages. Egg proportional survival was generally lower than at the free embryo stage in both substrate sizes; however, free embryo proportional survival was greater in small substrate trials. Rainbow darter total length did not affect the probability of lake sturgeon survival at either developmental stage. Results demonstrate that rainbow darters prey on early ontogenetic stages of lake sturgeon, corroborating previous results based on genetic diet analysis. Results fill a major knowledge gap concerning the vulnerability of pre-drift sturgeon to predation by an abundant river resident species that was previously discounted as a predator for early ontogenetic stages of lake sturgeon due to its small body size. 相似文献
20.
The composition and ecological role of ciliates and dinoflagellates were investigated at one station in Kongsfjorden, Svalbard,
during six consecutive field campaigns between March and December 2006. Total ciliate and dinoflagellate abundance mirrored
the seasonal progression of phytoplankton, peaking with 5.8 × 104 cells l−1 in April at an average chlorophyll a concentration of 10 μg l−1. Dinoflagellates were more abundant than ciliates, dominated by small athecates. Among ciliates, aloricate oligotrichs dominated
the assemblage. A large fraction (>60%) of ciliates and dinoflagellates contained chloroplasts in spring and summer. The biomass
of the purely heterotrophic fraction of the ciliate and dinoflagellate community (protozooplankton) was with 14 μg C l−1 highest in conjunction with the phytoplankton spring bloom in April. Growth experiments revealed similar specific growth
rates for heterotrophic ciliates and dinoflagellates (<0–0.8 d−1). Food availability may have controlled the protozooplankton assemblage in winter, while copepods may have exerted a strong
control during the post-bloom period. Calculations of the potential grazing rates of the protozooplankton indicated its ability
to control or heavily impact the phytoplankton stocks at most times. The results show that ciliates and dinoflagellates were
an important component of the pelagic food web in Kongsfjorden and need to be taken into account when discussing the fate
of phytoplankton and biogeochemical cycling in Arctic marine ecosystems. 相似文献