共查询到20条相似文献,搜索用时 31 毫秒
1.
Robert B. Page Claire Conarroe Diana Quintanilla Andriea Palomo Joshua Solis Ashley Aguilar Kelly Bezold Andrew M. Sackman David M. Marsh 《Ecology and evolution》2020,10(18):9948-9967
Climate change poses several challenges to biological communities including changes in the frequency of encounters between closely related congeners as a result of range shifts. When climate change leads to increased hybridization, hybrid dysfunction or genetic swamping may increase extinction risk—particularly in range‐restricted species with low vagility. The Peaks of Otter Salamander, Plethodon hubrichti, is a fully terrestrial woodland salamander that is restricted to ~18 km of ridgeline in the mountains of southwestern Virginia, and its range is surrounded by the abundant and widespread Eastern Red‐backed Salamander, Plethodon cinereus. In order to determine whether these two species are hybridizing and how their range limits may be shifting, we assessed variation at eight microsatellite loci and a 1,008 bp region of Cytochrome B in both species at allopatric reference sites and within a contact zone. Our results show that hybridization between P. hubrichti and P. cinereus either does not occur or is very rare. However, we find that diversity and differentiation are substantially higher in the mountaintop endemic P. hubrichti than in the widespread P. cinereus, despite similar movement ability for the two species as assessed by a homing experiment. Furthermore, estimation of divergence times between reference and contact zone populations via approximate Bayesian computation is consistent with the idea that P. cinereus has expanded into the range of P. hubrichti. Given the apparent recent colonization of the contact zone by P. cinereus, future monitoring of P. cinereus range limits should be a priority for the management of P. hubrichti populations. 相似文献
2.
Ten tetranucleotide microsatellite loci were isolated and characterized from Indiana (USA) populations of the smallmouth salamander, Ambystoma texanum. As opposed to individually labelling primers that were not yet known to be polymorphic, we used fluorescent dUTP to assess genetic variability and found it to be very effective. Allelic diversity ranged from two to 18 alleles per locus and observed heterozygosity ranged from 0.17 to 0.91 among roughly 25 individuals. One of the 10 markers also amplified and was polymorphic in the tiger salamander (A. tigrinum). 相似文献
3.
Wesley K. Savage 《Conservation Genetics》2009,10(3):619-622
The imperiled Santa Cruz long-toed salamander (Ambystoma macrodactylum croceum) is a geographically isolated lineage of the long-toed salamander species complex. Recovery of this taxon requires understanding
its population genetic structure, which is necessary to identify critical habitat. To assess the population structure of A. m. croceum, I developed primers for 17 microsatellite loci and evaluated their performance. Polymorphism ranged from 3 to 10 alleles
per locus, with an average of 6.3 per locus. Mean observed and expected heterozygosity were similar (H
O = 0.678; H
E = 0.708), with one locus demonstrating heterozygote deficiency. The performance of these and previously identified microsatellite
loci were also characterized for two other long-toed salamander lineages, as well as two other species of Ambystoma. The utility of these markers has similar and overlapping applications for conservation and population genetics studies of
other Ambystoma taxa, and will provide a basis for revising the recovery plan for A. m. croceum, particularly for designating corridors among interacting breeding populations and upland areas, and identifying areas where
gene flow should be restored. 相似文献
4.
Ten tetranucleotide and one dinucleotide polymorphic microsatellite loci were cloned and characterized for the Fire salamander (Salamandra salamandra) from 34 populations in Germany. A high genetic diversity (5–22 alleles per locus) and heterozygosity (40.6–95.2%) were observed for these markers. Chord distances for population comparisons of the western evolutionary recolonization lineage in the area near Cologne ranged from 0.139 to 0.366, whereas population comparisons between the western and eastern lineage ranged from 0.541 to 0.670. When compared with classical isolation methods, a sufficient number of polymorphic microsatellites can be obtained for the Fire salamander only from specially enriched sublibraries. 相似文献
5.
Seven polymorphic microsatellite markers were isolated and characterized for golden‐striped salamander, Chioglossa lusitanica (Bocage 1864), a salamandrid endemic to the northwestern part of the Iberian Peninsula, from genomic libraries enriched for (GATA)8 (GACA)8 (ATG)9 and (CA)16. These loci were screened in 37–51 individuals from two populations. The number of alleles per locus ranged from five to 19. Heterozygosity ranged from 0.241 to 1.0. The high level of polymorphism revealed by these loci will be extremely useful for the study of population structure and evolutionary history of this species. 相似文献
6.
Eleven tetranucleotide microsatellite loci were developed for the Del Norte salamander (Plethodon elongatus). The loci were variably polymorphic, ranging from two to 20 alleles per locus, with expected heterozygosities ranging from 0.07 to 0.86. The loci also amplified in a congener, the Siskiyou Mountain salamander (P. stormi). The microsatellite loci will be used to assess the utility of highly polymorphic markers to assay within‐ and between‐species differentiation between these two closely related species. 相似文献
7.
Dioon edule (Zamiaceae) is an endemic Mexican cycad. Nineteen microsatellite loci were isolated from three enriched genomic libraries of D. edule var. angustifolium, D. tomasellii, and D. caputoi. Seven of these loci showed polymorphisms in D. edule. Levels of polymorphism were assessed using 16 individuals from each of seven populations throughout the range of this species. The number of alleles per locus ranged from two to five and the observed and expected heterozygosities ranged from 0.0 to 0.9821 and from 0.0088 to 0.6318, respectively. All loci show significant linkage disequilibrium. Three loci depart significantly from Hardy–Weinberg equilibrium. 相似文献
8.
Frank E. Anderson 《Conservation Genetics》2007,8(4):965-975
Euchemotrema hubrichti (Pilsbry HA (1940) Volume 1, Part 2. Monographs of the Academy of Natural Sciences, Philadelphia, 3, I-VI, 2, 575–994, I-IX)
is a polygyrid land snail known only from the Larue Pine Hills region of southwestern Illinois, USA. Despite the restricted
range of the species, observations in the field suggested that patterns of genetic variation within E. hubrichti might show geographic structure. To assess the dispersal potential of E. hubrichti, a mark-recapture experiment was performed at two sites <100 meters apart. To evaluate the geographic distribution of genetic
variation, a region of the mitochondrial cytochrome c oxidase subunit I gene was sequenced from 53 snails collected from sites
spanning the species range. Mark-recapture experiments suggested that population sizes at two sites were low and migration
between sites was not detected. AMOVA and nested clade analysis (NCA) revealed significant genetic structure among sites.
Two sites in particular appeared to be isolated from the others based on nested clade analysis. AMOVA also detected significant
genetic differentiation among sites, but partitioning the data into two groups (populations 1 and 2) based on the NCA results
did not explain a significant amount of genetic variation. Coalescent methods suggested that population sizes and migration
rates for populations 1 and 2 are unequal. These patterns are likely due to the low dispersal ability of E. hubrichti and patchiness of preferred E. hubrichti habitat rather than an ancient vicariant event. These preliminary findings have implications both for the conservation of
this species and for the population genetics of any spatially restricted endemic species with low dispersal ability. 相似文献
9.
S. G. Mech A. Storfer J. A. Ernst M. W. Reudink S. C. Maloney 《Molecular ecology resources》2003,3(1):79-81
Microsatellites have been developed for few amphibian species. However, developing genetic markers for population genetic studies in amphibians is critical because amphibians are declining globally. The tiger salamander, Ambystoma tigrinum, is widespread throughout the United States and includes the endangered subspecies, A. t. stebbinsi. We present primers and amplification conditions for 10 polymorphic microsatellite loci that have produced successful results in three subspecies of A. tigrinum. Number of alleles per locus ranged from one to 11 and heterozygosity ranged from 0 to 0.815 depending on the subspecies and locus analysed. These markers should prove useful for future studies of genetic diversity and population subdivision. 相似文献
10.
Estimating levels of gene flow and assessing levels of population connectivity are of critical importance to the field of conservation genetics, especially for imperiled species. Many factors can influence dispersal and therefore gene-flow patterns across a natural landscape. These patterns can be substantially altered by the impacts of habitat modification by humans or natural phenomena. Landscape-genetic studies that address both historical and contemporary influences on gene flow can be critical to demonstrating whether isolated populations with low levels of genetic variation are typical of the species or a result of strong negative effects of such modification. We used 10 microsatellite markers to investigate the spatial genetic patterns of the Red Hills salamander (Phaeognathus hubrichti), a federally listed species. Bayesian clustering revealed five well-supported demes within the range of P. hubrichti. Gene-flow analysis suggested that overall migration levels for P. hubrichti are low, but coalescent methods indicate that migration levels were significantly higher before habitat modification by humans. By accounting for history and species characteristics, our results suggest that loss and fragmentation of habitat have strongly negatively affected P. hubrichti by reducing migration, increasing bottlenecks, and promoting high levels of inbreeding. 相似文献
11.
Stacey L. Lance Kenneth L. Jones Cris Hagen Travis C. Glenn J. Matthew Jones J. Phil Gibson 《Conservation Genetics》2009,10(6):1907-1910
We isolated and characterized 19 microsatellite loci from the endangered seaside alder, Alnus maritima. Loci were screened in 24 individuals of A. maritima and four individuals of its congener the hazel alder, A. serrulata. The number of alleles per locus ranged from 2 to 6, observed heterozygosity ranged from 0 to 0.952, and the probability
of identity values ranged from 0.126 to 0.850. These new loci provide tools for characterizing the population genetics of
this rare tree. 相似文献
12.
Xiuli Wang Teng Feng Lei Yang Chengcheng Liu Xiangying Meng Xuemei Qiu 《Conservation Genetics》2009,10(3):729-731
Sea urchin is an important model organism for evolutionary biology, embryology, and gene regulation study. We developed and
evaluated simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) of Strongylocentrotus
purpuratus and Hemicentrotus pulcherrimus. Characteristics of nine EST-SSR loci were investigated using 41 Hemicentrotus pulcherrimus individuals. The number of alleles per locus ranged from two to five. The observed heterozygosity ranged from 0.122 to 0.7805, while the
expected heterozygosity ranged from 0.4472 to 0.7696. These loci and markers will be useful for population genetics and systemic
evolution among species of sea urchin. 相似文献
13.
Freshwater molluscs are globally critically threatened, yet little is known about their genetic diversity. We describe the
isolation and characterization of the first microsatellite markers for the European swan mussel, Anodonta cygnea. Nine loci were polymorphic in a panel of 110 individuals from seven German and Estonian populations. Number of alleles ranged
from 2 to 14 and heterozygosity levels ranged from 0.021 to 0.498 for H
o and from 0.069 to 0.634 for H
e. Deficiency of heterozygous genotypes was observed in six loci. The length of the microsatellite repeat motives was positively
correlated with the allele numbers in the respective loci. 相似文献
14.
Hye Suck An Jung Youn Park Mi-Jung Kim Eun Young Lee Kyung Kil Kim 《Conservation Genetics》2009,10(6):1969-1972
Here we report development and characterization of 14 polymorphic microsatellite loci from Sebastes schlegel. Polymorphism at these loci revealed from 3 to 23 alleles. The observed heterozygosity ranged from 0.34 to 1.00, and the
expected heterozygosity ranged from 0.31 to 0.95. No linkage disequibrium was found. Two loci were significantly deviated
from HWE (P < 0.01). The 14 loci were also surveyed in four other Sebastes species and 12 loci successfully amplified, where allelic diversity ranged from highly polymorphic to monomorphic. These
results demonstrate these microsatellite markers can be used for the study of intra- and inter-specific genetic diversity. 相似文献
15.
Patinopecten yessoensis is a kind of cold water shellfish, and is an important economic species in China. In our study, we developed and evaluated
simple sequence repeat (SSR) markers of P. yessoensis. Characteristics of 11 EST-SSR loci were investigated using 40 P. yessoensis individuals. The number of alleles per locus ranged from two to five. The observed heterozygosity ranged from 0.1026 to 0.9487, while
the expected heterozygosity ranged from 0.1865 to 0.7433. All loci except P4 departed from Hardy–Weinberg equilibrium (P < 0.05) significantly. There was no LD observed between all pairs of EST-SSRs loci. These loci and markers will be useful
for population genetics and systemic evolution of scallop. 相似文献
16.
Obscure puffer (Takifugu obscurus) is an anadromous fish species in China. Here, we reported 10 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of T. obscurus. The number of alleles, observed and expected heterozygosity per locus in 30 individuals ranged from four to 10, from 0.57
to 0.86 and from 0.68 to 0.90, respectively. Three loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni
correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species amplification of these
microsatellite loci in additional three fish species was performed. These polymorphic microsatellite loci would be useful
for investigating genetic population structure and construction of genetic linkage map in T. obscurus. 相似文献
17.
Xiuli Wang Xiaoli Guo Yingming Zhang Xiangying Meng Xuemei Qiu Shaozhen Liu Tianxiao Zhang 《Conservation Genetics》2009,10(5):1455-1457
In our study, we evaluated simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) of Fenneropenaeus chinensis. Characteristics of 11 EST-SSR loci were investigated using 34 F. chinensis individuals. The number of alleles per locus ranged from 4 to 9. The observed heterozygosity ranged from 0.118 to 0.826,
while the expected heterozygosity ranged from 0.623 to 0.847. Two loci (T2 and T12) conformed to the Hardy–Weinberg equilibrium.
There was no LD observed between all pairs of EST-SSRs loci. The PIC values of 11 microsatellite loci were higher than 0.5.
These loci and markers will be useful for population genetics and systemic evolution of F. chinensis. 相似文献
18.
The red‐legged partridge, Alectoris rufa, is an endemic species of the southwestern Mediterranean, and the most popular game bird in the Iberian Peninsula. A total of 27 microsatellite loci was isolated from an enriched genomic library of A. rufa. Six perfect GT microsatellites were characterized and optimized in 45 individuals of A. rufa. All loci revealed high levels of polymorphism with a number of alleles that ranged from three to 13. Observed heterozygosity ranged from 0.2 to 0.6. Cross‐species amplification showed that all loci were also polymorphic in rock partridge, Alectoris graeca. The new markers will be useful in determining hybridization between both species of Alectoris. 相似文献
19.
M. C. CAILLAUD G. MONDOR‐GENSON S. LEVINE‐WILKINSON L. MIEUZET A. FRANTZ J. C. SIMON A. COEUR D'ACIER 《Molecular ecology resources》2004,4(3):446-448
Microsatellite loci were isolated from enriched partial genomic libraries of Acyrthosiphon loti and Acyrthosiphon pisum. Twenty of those loci were characterized in A. pisum. Fifteen of those loci were polymorphic. Genetic diversity varied across loci, allele repeat number ranging from two to 15, and observed heterozygosity from 0.1 and 0.96. An additional eight microsatellite loci originally isolated from other aphids but cross‐priming with A. pisum showed polymorphism as well. Allele size ranged from three to 9 and observed heterozygosity from 0.43 to 0.84. Overall, we present 23 microsatellite loci that can be used to reveal polymorphism in pea aphids. 相似文献
20.
Stacey L. Lance Cris Hagen Travis C. Glenn Robb T. Brumfield Katherine Faust Stryjewski Gary R. Graves 《Conservation Genetics》2009,10(4):1195-1198
We isolated and characterized 15 microsatellite loci from the endemic Jamaican streamertail hummingbird Trochilus polytmus. Loci were screened in 12 individuals of both T. polytmus and its sister species T. scitulus, also a Jamaican endemic. The number of alleles per locus ranged from 2 to 10, observed heterozygosity ranged from 0 to 1,
and the probability of identity values ranged from 0.038 to 0.663. These new loci provide tools for characterizing the narrow
hybrid zone between the two species. 相似文献