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1.
C. G. N. de Vooys 《Helgoland Marine Research》2006,60(3):239-242
Blue mussels (Mytilus edulis) living in estuaries have to cope with varying concentrations of suspended sand. Sand flowing through the inhalant siphons comes into the infrabranchial chamber. The inhalant siphon can be partially closed by the branchial membrane. As a result the inward flow decreases, and suspended sand sinks and can be eliminated. Experiments with mussels from three ecologically different locations showed about the same response of the branchial membrane on contact with suspended sand. The presence and function of the branchial membrane appears to be an adaptation of mussels to their estuarine environment. 相似文献
2.
R. K. Pipe 《Cell and tissue research》1990,261(2):261-268
Summary Pre- and post-embedding techniques were used to investigate the ultrastructural binding of a range of lectins to the haemocytes of the mussel Mytilus edulis. Direct and indirect labelling procedures were employed using colloidal gold and ferritin-labelled lectins, or biotinylated lectins followed by gold-labelled streptavidin. Cell surface receptors were present for lectins from Helix pomatia (HPA), Helix aspersa (HAA), Triticum vulgaris (WGA) and Tetragonolobus purpureas (TPA). Double labelling of haemocytes with HPA and WGA demonstrated binding sites for both lectins on the plasma membrane of the majority of haemocytes. Endocytosis of colloidal gold-labelled HPA was observed for unfixed haemocytes. Three classes of haemocyte were identified by use of morphological criteria: hyalinocytes; granulocytes containing small granules; and granulocytes containing large granules. Lectin binding showed the small granules of the granulocytes to be HPA-positive and the large granules of the granulocytes to be WGA-positive. The WGA-positive granules demonstrated a differential pattern of binding according to granule size. Binding sites for the lectin from Arachis hypogaea (PNA) were not demonstrated on the cell surface, but did show an affinity for the heterochromatin region of the nucleus in post-embedding protocols. 相似文献
3.
Blue mussel hemocytes (cells with immunoresponse activities) are suggested as indicators of anthropogenic contamination. We compared hemocyte numbers, granulocytoma (aggregated hemocytes), and parasites among populations of mussels from different areas of Skagerrak (a north and a south), seasons (summer and autumn), and impact levels (close or far from industrial activities). Seasonal hemocyte numbers were larger in the north compared to the south. Northern unimpacted populations had higher hemocyte numbers than populations close to industries, while no differences were found in the south. More uneven tissue distributions were found in populations far from industries in the north area and in populations close to industries in the south area. Parasites were more common in northern mussels than in southern, but no relationship to impact level was found. Mussels with granulocytoma, however, were found in all populations from the impacted sites while in none of the other populations suggesting granulocytoma as a possible indicator of industrial impact. 相似文献
4.
One of the most effective techniques for evaluating stress is the analysis of developmental stability, measured by stochastic
variation based particularly on fluctuating asymmetry, i.e. a variance in random deviations from perfect bilateral symmetry.
However, the application of morphological methods is only possible when an organism lives under testing conditions during
a significant part of its ontogenesis. Contrary to morphological characters, behavior can change very fast. Consequently,
methods based on behavioural characters may have advantages over more traditional approaches. In this study we describe the
technique of assessing stochastic variation, using not morphological, but behavioural characters. To measure stochastic variation
of behavioural response, we assessed the stability of the isolation reaction of blue musselMytilus edulis at regular changes of salinity. With increasing temperature from +12°C to +20°C stochastic variation of the isolation reaction
increased, which is a common response to change of environmental conditions. In this way, we have developed a method of assessing
stochastic variation of behavioural response in molluscs. This method may find a great range of applications, because its
usage does not require keeping animals in tested conditions for a long time. 相似文献
5.
The role of the blue mussel Mytilus edulis in the cycling of nutrients in the Oosterschelde estuary (The Netherlands) 总被引:1,自引:0,他引:1
The fluxes of particulate and dissolved material between bivalve beds and the water column in the Oosterschelde estuary have been measured in situ with a Benthic Ecosystem Tunnel. On mussel beds uptake of POC, PON and POP was observed. POC and PON fluxes showed a significant positive correlation, and the average C:N ratio of the fluxes was 9.4. There was a high release of phosphate, nitrate, ammonium and silicate from the mussel bed into the water column. The effluxes of dissolved inorganic nitrogen and phosphate showed a significant correlation, with an average N:P ratio of 16.5. A comparison of the in situ measurements with individual nutrient excretion rates showed that excretion by the mussels contributed 31–85% to the total phosphate flux from the mussel bed. Ammonium excretion by the mussels accounted for 17–94% of the ammonium flux from the mussel bed. The mussels did not excrete silicate or nitrate. Mineralization of biodeposition on the mussel bed was probably the main source of the regenerated nutrients.From the in situ observations net budgets of N, P and Si for the mussel bed were calculated. A comparison between the uptake of particulate organic N and the release of dissolved inorganic N (ammonium + nitrate) showed that little N is retained by the mussel bed, and suggested that denitrification is a minor process in the mussel bed sediment. On average, only 2/3 of the particulate organic P, taken up by the mussel bed, was recycled as phosphate. A net Si uptake was observed during phytoplankton blooms, and a net release dominated during autumn. It is concluded that mussel beds increase the mineralization rate of phytoplankton and affect nutrient ratios in the water column. A comparison of N regeneration by mussels in the central part of the Oosterschelde estuary with model estimates of total N remineralization showed that mussels play a major role in the recycling of nitrogen. 相似文献
6.
Vertebrate-type sex steroids have been detected in a number of mollusk species and may play a role in the reproductive physiology of the animal. Mollusks are also exposed to exogenous estrogenic steroids that are present in sewage effluents, and these may add to the estrogenic burden of exposed animals. We investigated the uptake of estrogens in the blue mussel, Mytlius edulis and report for the first time the identity of estrogen fatty acid ester metabolites formed in vivo in an invertebrate. We exposed mussels to waterborne radiolabeled [(14)C]-17beta-estradiol (E2) or estrone (E1) and determined the nature of their metabolites using radio-HPLC and mass spectrometry (MS). After 13 days of exposure to 10ng/L E2, concentrations of radiolabeled residues were 2428-fold higher in M. edulis soft tissues compared with the ambient water concentration of E2. All the E2 residues in the mussel were present as a lipophilic ester which, in depuration studies, had a half-life of 8.3 days. Exposure of mussels to [(14)C]-E1 (70ng/L) resulted in formation of a similar lipophilic metabolite that after hydrolysis released [(14)C]-E2. Tandem MSMS analyses of the purified steroid ester fraction isolated from mussels exposed to either E2 or E1 revealed that they had the same composition and comprised C16:0, C16:1 and C16:2 esters of E2. This work reveals that in vivo E1 is rapidly metabolized to E2 in mussels prior to conjugation to C16 fatty acid esters, proving that C17-ketoreductase and C16 fatty acid acyl-CoA:E2 acyltransferase are important enzymes for the metabolism of estrogens in M. edulis. 相似文献
7.
Sergey Dobretsov Martin Wahl 《Journal of experimental marine biology and ecology》2008,355(2):137-144
The mussel Mytilus edulis settlement and distribution was studied on plastic panels with manipulated flow regime (faired, bluff, split and angled) with or without water soluble metabolites of the green alga Cladophora rupestris. The panels were exposed vertically on a device (hydrovane) that ensures their constant orientation in the current during the peak of larval settlement at 1 m depth. In order to investigate larval distribution on the panels, half of them were coated with a silicone vacuum grease that prevents larvae from de-attachment. This grease was not toxic and did not attract or repel larvae. Low densities of larvae on the un-greased plates compared to the greased ones suggested that some of larvae left the substratum. The blue mussel larvae initially settled in regions of reduced shear velocity and then redistribute to the regions of high shear velocity. The presence of the alga increased the density of blue mussel larvae and changed their distribution on the panels. Overall, our results demonstrated that larval recruitment of M. edulis is an active process affected both by boundary-layer hydrodynamics and algal waterborne compounds. 相似文献
8.
The results demonstrate the first attempt to determine metallothionein turnover in the whole soft tissues of mussels Mytilus edulis exposed to cadmium. Half-lives for metallothionein and cadmium are 25 and 300 days, respectively. As metallothionein degrades the released cadmium induces further synthesis of the protein, to which the metal becomes resequestered. The slow metallothionein turnover rates (compared with mammals) and the lack of significant cadmium excretion testify to the relatively stable nature of the cadmium-metallothionein complex in these invertebrates and supports the view of a detoxifying role for metallothionein in the mussels. 相似文献
9.
Dolmer P. Kristensen T. Christiansen M. L. Petersen M. F. Kristensen P. S. Hoffmann E. 《Hydrobiologia》2001,465(1-3):115-127
The short-term effect of mussel dredging in a brackish Danish sound was studied. A commercial dredging track was identified and an analysis of the species composition inside the track and at an adjacent control area showed that dredging changed the community structure by reducing the density of polychaetes. In order to investigate the extent and the duration of the dredging impact experimental dredging was conducted. The experimental dredging removed 50% of the mussels in two dredged areas. Immediately after dredging, a significantly lower number of species was measured inside the mussel beds in dredged areas compared to control and boundary areas. This effect lasted for at least 40 days. The analysis of the species composition showed that the dredged area had a significantly lower density, particularly of polychaetes compared to the boundary area. An increased number of species was recorded outside the mussel beds just after dredging, but this effect lasted for less than 7 days. After dredging, brown shrimps, C. crangon invaded the dredged areas. This species is an important predator of smaller invertebrates, and it is suspected that it was feeding on small vulnerable polychaetes exposed at the sediment surface after dredging. The dredging process was observed to form 2–5-cm deep furrows in the seabed, but the sediment texture and the organic content of the sediment was not affected. The biomass accumulation of individual blue mussels was significantly lower in the dredged area compared to the boundary area. This indicates that the disturbance of the mussel bed structure reduced growth and that the lowering of intraspecific food competition caused by a reduced density of mussels did not increase the accumulation of biomass in the mussels which remained in the dredged area. 相似文献
10.
Ji Young Cho Eun-Hee Kwon Jae-Suk Choi Sung-Youl Hong Hyun-Woung Shin Yong-Ki Hong 《Journal of applied phycology》2001,13(2):117-125
Twenty-seven species of common seaweeds from the coast of Korea havebeen screened for antifouling activity. The seaweed extracts were tested inlaboratory assays against the marine fouling green alga Enteromorphaprolifera and the blue mussel Mytilus edulis. Tissue growth, sporesettlement, zygote formation and germlings of the E. prolifera wereinhibited by methanol extracts of the seaweed Ishige sinicola (= I. foliacea) and Sargassum horneri. Spore settlement was stronglyinhibited by using extract concentrations as low as 30 g mL-1with I. sinicola and 120 g mL-1 with S. horneri. The repulsive activity of the foot of the mussel was completely inhibited bymethanol extracts of I. sinicola and Scytosiphon lomentaria atconcentrations of 40 g per 10 L drop supplied to eachmussel. These extracts also showed strong antifouling activities onlarval settlement with, respectively, no or only 6% of the spat settlingwhen a test concentration of 0.8 mg mL-1 was used. This work isthe first stage towards the development of novel antifouling agents frommarine macroalgae. 相似文献
11.
On the dynamics of the stocks of blue mussels (Mytilus edulis L.) in the Danish Wadden Sea 总被引:4,自引:0,他引:4
As biological basis for the monitoring programme for the commercially exploited stock(s) of mussels (Mytilus edulis L.) in the Danish Wadden Sea, samples of mussels have been collected regularly since 1986, both from sub-tidal and inter-tidal mussel beds. These samples are the basis for the estimation of total biomass. They also provide data on size frequency distributions, which have been analysed for cohort identification resulting in length at age data, which again have been used for estimating parameters (L
and K) for the von Bertalanffy growth equation (VBGE) as well as mortality parameters. By applying these in the Beverton & Holt model, estimates of average biomass and annual production (P) of the mussels have been obtained together with possible fisheries yields from the beds. The growth and mortality parameters and the figures for annual production and P/B are compared with figures from other investigations. These analyses have been the basis for annual assessments of the mussel stocks, which again are used in the current management of mussel fishery in the Danish Wadden Sea. 相似文献
12.
Lipopolysaccharide and opioids activate distinct populations of Mytilus edulis immunocytes 总被引:3,自引:0,他引:3
T. K. Hughes Jr. E. M. Smith J. A. Barnett R. Charles G. B. Stefano 《Cell and tissue research》1991,264(2):317-320
Summary Studies in Mytilus edulis have indicated that immunoregulatory activities comoparable to those in vertebrates also exist in invertebrates. Mytilus immunocytes resemble cells of the vertebrate monocyte/macrophage lineage and are activated by similar substances. We searched for differential effects of opioids on these cells in comparison with those of lipopolysaccharide (LPS), in order to determine if different subpopulations of immunoactive hemocytes are involved. We showed that Mytilus immunocytes respond to LPS in a fashion similar to that in vertebrate granulocytes by flattening, and increasing in cellular perimeter and mobility, that LPS administered in vivo results in a lowering of the number of free hemocytes that can be obtained from the animal, and that distinct immunoactive cell populations seem to exist since apparently different subsets of cells react when exposed to LPS or opioids and the opioid antagonist naloxone. 相似文献
13.
The growth of blue mussels (Mytilus edulis) was studied in the landlocked bay Hopavågen in central Norway for 3 years, of which in 2 years (1998 and 1999) nutrients were added to increase the primary production. Nutrients (N:Si:P) were added daily from May to October in 1998 (molar ratio 15:5:4.1) and 1999 (molar ratio 16:8:1). The doses of nutrients correspond to 0.4 and 0.8 g P l–1 day–1 in 1998 and 1999 respectively. The growth of blue mussels (Mytilus edulis) in 1997 was followed at four depths (1, 2, 4 and 7 m). In 1998 and 1999 growth was followed at 2 and 10 m depths at four locations in Hopavågen and at a control station outside on the coast. The nutrient supply in 1998 only slightly increased the algal biomass (chlorophyll a), whereas mean daily primary production during the summer remained at the same level as the previous year. The increased nutrient supply in 1999 caused a nearly 50 and 100% increase in mean summer biomass and daily primary production, respectively. The growth of blue mussels in Hopavågen in 1997 and 1998 was within the same size range during the summer. In 1999 the shell length of blue mussels kept at 2 m depth was significantly higher than in the previous year at end of the growth season. The recorded growth was also significant higher than for mussels at 2 m depth at the control station. No difference in shell length was observed on mussels grown at 10 m depth in Hopavågen and in the control stations in 1998 and 1999. A higher tissue content was found in blue mussels grown at 2 m depth in Hopavågen, both in 1998 and 1999 when compared to the control groups. At 10 m depth no differences were recorded. 相似文献
14.
Dr. M. N. Moore 《Cell and tissue research》1976,175(3):279-287
Latent beta-glucuronidase and glucosaminidase activities have been demonstrated in small cytoplasmic particles, which may possibly be primary lysosomes, as well as some larger granules of the digestive cells of the common mussel. Latency was indicated by increased staining of these structures following incubation in buffer at pH 4.5 at 37 degrees C. The exposure of mussels to temperatures of 25-28 degrees C over a period of four days induced a significant decrease in the latency of lysosomal glucosaminidase. Thermal death produced labilization of lysosomes although selective release of hydrolase activity was indicated by the differential latency of glucosaminidase and glucuronidase. The injection of hydrocortisone induced a significant increase in latency in stressed animals, indicating that the stress response involved changes in structure and function of membranes. 相似文献
15.
Paul A.X. Bologna Michele L. Fetzer Erin M. Moody 《Journal of experimental marine biology and ecology》2005,316(2):117-131
Coastal marine seagrass ecosystems are important nursery grounds for commercially and recreationally important species, and they serve as key settlement and recruitment sites for other species. We investigated several years (2001-2003) where episodic settlement events of blue mussels (Mytilus edulis) occurred in Barnegat Bay, NJ, USA. Population assessment indicated that blue mussels settled in eelgrass beds (Zostera marina) in late spring with peak densities exceeding 170,000 m−2. Based on calculated filtration rates of M. edulis, we determined that for at least 53 days in 2001, the density and size distribution of M. edulis were sufficient to filter the water column volume in excess of twice a day, with maximum calculated filtration rates exceeding 8 m3 water m−2 day−1. While the settlement event in 2001 was very localized, in 2003, the settlement event was considerably more widespread throughout the bay, with maximum settling densities exceeding 175,000 individuals m−2. Associated with these high densities, maximum calculated filtration rates exceeded 15 m3 water m−2 day−1. This filtration potential may have impeded the localized development of a brown-tide (Aureococcus anophagefferens) bloom in 2001, which occurred in other regions of the bay, but the widespread settlement event seen in 2003 may have impeded the development of any brown-tide blooms in Barnegat Bay during that summer. The decline in mussel densities throughout the summer may be a result of elevated water temperatures in this back bay, but at one site, the high settlement of M. edulis was followed by a substantial migration (>40 individuals m−2) of small sea stars (Asterias forbesii). In 2001, A. forbesii was a significant factor in reducing M. edulis density by the end of the summer at the Barnegat Inlet site and a community level assessment showed significant positive correlations between mussel aggregations and sea star densities (r=0.68-0.73, P<0.001). At this same site in 2003, the sea stars were again present in high densities (26 m−2) and were a potential mechanism for mussel decline. In other regions of the bay, sea star densities are very low, but numerous other predatory species exist, including blue crabs (Callinectes sapidus), green crabs (Carcinus maenus), spider crabs (Libinia spp.), and several Xanthid crabs. Given the high mussel densities seen in this study and the considerable predation by sea stars and other benthic predators, the benthic-pelagic coupling which these mussels provide in this system contributes to the high secondary production in these grass beds. 相似文献
16.
Summary The main iron-binding protein in the hepatopancreas of the musselMytilus edulis, which had been previously iron-loaded by exposure to carbonyl iron (spheres of elemental iron less than 5 m diameter), has been isolated to electrophoretic purity and identified as ferritin. This ferritin hasM
r, of 480000, pI of 4.7–5.0 and is composed of two subunits,M
r 18500 andM
r 24600. Under the electron microscope, it appears as electron-dense iron cores of average diameter 5 nm surrounded by a polypeptide shell to a final average overall diameter of 11 nm. The purified protein contains, on average, 200 iron atoms/molecule protein. On immunodiffusion,M. edulis hepatopancreas ferritin gives a partial cross-reaction with antiserum to horse spleen ferritin and lamprey (Geotria australis) liver ferritin but does not react with antiserum to chiton (Acanthopleura hirtosa) haemolymph ferritin. 相似文献
17.
A process for efficient production of an alkaline β-mannanases from Bacillus sp. N16-5 was established by heterologous expression using Pichia pastoris. A high producing strain was generated by removing the native β-mannanases signal peptide and increasing the copy number of the mature β-mannanases gene. High cell density fermentation of this strain in 1-L bioreactor led to a production level of 4164 U/mL after 96 h of induction. Sorbitol co-feeding and temperature-lowering strategies both increased the β-mannanase production levels. Combined usage of these two strategies achieved the most effective result—the enzyme level reached 6336 U/mL within 84 h, which to our best knowledge is the highest production level reported for the expression of extreme β-mannanase thus far. The strategy described in this work can also be adapted to express other important industrial enzymes with extreme properties. 相似文献
18.
Field surveys (dating back to 1950) and aerial photograph series (dating back to 1966) were evaluated to determine sites of
intertidal blue mussel (Mytilus edulis) beds at the Wadden Sea coast of Lower Saxony. Maps were prepared indicating sites of blue mussel beds during the last decades.
A table gives additional information on the presence (or absence) of blue mussel beds at each site at the time of large-scale
surveys. Altogether 187 sites of M. edulis beds were recorded in the investigation area. In spring 1996, there were still only 19 sites where mussel beds still occurred,
although at 51 sites residual mussel-bed structures were present, e.g. shell bases of former beds or protruding patches (which
had been occupied by M. edulis before the beds vanished) and open spaces. At that time, the majority of the sites contained neither mussel beds nor mussel-bed
structures. The analysis of recent data confirmed that mussel larvae have preferred to settle in sites of present mussel beds
and sites with bases of former mussel beds. There was no preferential selection of one of these categories (settled beds vs.
shell bases). On the other hand, the presence of mussel beds or mussel bed structures is not obligatory for settlement, because
sites without those structures were also re-settled by the spatfall in 1996, even though on a smaller scale. 相似文献
19.
The present study reports the recombinant expression, purification, and partial characterization of a typical aspartic proteinase from Arabidopsis thaliana (AtAP A1). The cDNA encoding the precursor of AtAP A1 was expressed as a functional protein using the yeast Pichia pastoris. The mature form of the rAtAP A1 was found to be a heterodimeric glycosylated protein with a molecular mass of 47 kDa consisting of heavy and light chain components, approx. 32 and 16 kDa, respectively, linked by disulfide bonds. Glycosylation occurred via the plant specific insert in the light chain. The catalytic properties of the rAtAP A1 were similar to other plant aspartic proteinases with activity in acid pH range, maximal activity at pH 4.0, Km of 44 μM, and kcat of 55 s−1 using a synthetic substrate. The enzyme was inhibited by pepstatin A. 相似文献
20.
Optimal foraging versus shared doom effects: interactive influence of mussel size and epibiosis on predator preference 总被引:1,自引:0,他引:1
Peter Enderlein Stefanie Moorthi Holger Röhrscheidt Martin Wahl 《Journal of experimental marine biology and ecology》2003,292(2):231-242
In the western Baltic Sea, the highly competitive blue mussel Mytilus edulis tends to monopolize shallow water hard substrata. In many habitats, mussel dominance is mainly controlled by the generalist predator Carcinus maenas. These predator-prey interactions seem to be affected by mussel size (relative to crab size) and mussel epibionts.There is a clear relationship between prey size and predator size as suggested by the optimal foraging theory: Each crab size class preferentially preys on a certain mussel size class. Preferred prey size increases with crab size.Epibionts on Mytilus, however, influence this simple pattern of feeding preferences by crabs. When offered similarly sized mussels, crabs prefer Balanus-fouled mussels over clean mussels. There is, however, a hierarchy of factors: the influence of attractive epibiotic barnacles is weaker than the factor ‘mussel size’. Testing small mussels against large mussels, presence or absence of epibiotic barnacles does not significantly alter preferences caused by mussel size. Balanus enhanced crab predation on mussels in two ways: Additional food gain and, probably more important, improvement in handling of the prey. The latter effect is illustrated by the fact that artificial barnacle mimics increased crab predation on mussels to the same extent as do live barnacles.We conclude that crab predation preferences follows the optimal foraging model when prey belong to different size classes, whereas within size classes crab preferences is controlled by epibionts. 相似文献